##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364597_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2497327 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.615610210436998 31.0 30.0 33.0 26.0 34.0 2 29.758923440943057 31.0 30.0 34.0 26.0 34.0 3 29.73717859134987 31.0 30.0 34.0 26.0 34.0 4 33.42840925517564 35.0 33.0 37.0 28.0 37.0 5 32.807499378335315 35.0 33.0 37.0 26.0 37.0 6 32.80059559681211 35.0 33.0 37.0 26.0 37.0 7 32.608988730750916 35.0 33.0 37.0 25.0 37.0 8 32.54404449237124 35.0 33.0 37.0 25.0 37.0 9 33.85683012276726 38.0 33.0 39.0 24.0 39.0 10-11 33.64135213370135 37.0 33.0 39.0 22.5 39.0 12-13 33.50286306118502 37.0 33.0 39.0 20.5 39.0 14-15 34.49386263793248 38.0 33.0 41.0 21.5 41.0 16-17 34.400139829505704 38.0 33.0 41.0 20.0 41.0 18-19 34.430018976289446 38.5 33.0 41.0 19.0 41.0 20-21 34.452443352432425 39.0 33.0 41.0 19.0 41.0 22-23 34.433825846595184 39.0 33.0 41.0 19.0 41.0 24-25 34.42783203801504 39.0 33.0 41.0 20.0 41.0 26-27 34.32910287679587 39.0 33.0 41.0 19.0 41.0 28-29 34.10055271095855 39.0 33.0 41.0 18.0 41.0 30-31 34.05199759582946 38.0 33.0 41.0 18.0 41.0 32-33 33.893005000947014 38.0 33.0 41.0 17.5 41.0 34-35 33.7682249861552 38.0 33.0 41.0 16.0 41.0 36-37 33.61216933144919 38.0 33.0 40.0 16.0 41.0 38-39 33.4873260490116 38.0 32.5 40.0 15.0 41.0 40-41 33.32361120510049 38.0 32.0 40.0 12.5 41.0 42-43 33.163415724092204 38.0 32.0 40.0 9.0 41.0 44-45 32.97093912811578 38.0 32.0 40.0 8.5 41.0 46-47 32.74778132779568 38.0 31.5 40.0 6.0 41.0 48-49 32.52344666917868 38.0 31.0 40.0 2.0 41.0 50-51 32.34030525437798 38.0 31.0 40.0 2.0 41.0 52-53 32.181368719434815 38.0 31.0 40.0 2.0 41.0 54-55 31.977085099388265 37.0 30.5 40.0 2.0 41.0 56-57 31.754059640567696 37.0 30.0 40.0 2.0 41.0 58-59 31.571554506077895 37.0 30.0 40.0 2.0 41.0 60-61 31.362928042663214 36.5 30.0 40.0 2.0 41.0 62-63 31.102514808833604 36.0 29.0 40.0 2.0 41.0 64-65 30.83448262882674 36.0 29.0 39.0 2.0 41.0 66-67 30.56254587404853 35.0 29.0 39.0 2.0 41.0 68-69 30.254359160814744 35.0 29.0 39.0 2.0 40.5 70-71 29.996742316885214 35.0 29.0 38.0 2.0 40.0 72-73 29.842763082287583 35.0 29.0 37.5 2.0 40.0 74-75 29.558609665454306 35.0 29.0 37.0 2.0 39.0 76-77 29.257356165211846 34.5 29.0 36.5 2.0 39.0 78-79 28.95048766140758 34.0 28.0 36.0 2.0 39.0 80-81 28.67144751167949 34.0 28.0 36.0 2.0 37.5 82-83 28.406125429309014 34.0 28.0 35.0 2.0 37.0 84-85 28.38292622471947 34.0 28.5 35.0 2.0 37.0 86-87 28.229798700770864 34.0 28.5 35.0 2.0 36.0 88-89 28.08998341026225 34.0 28.0 35.0 2.0 36.0 90-91 27.930068429164464 34.0 28.0 35.0 2.0 36.0 92-93 27.79337707877262 34.0 27.5 35.0 2.0 35.5 94-95 27.705141737545784 34.0 27.0 35.0 2.0 35.0 96-97 27.623371709031296 34.0 27.0 35.0 2.0 35.0 98-99 27.530409513852213 34.0 27.0 35.0 2.0 35.0 100-101 27.43520191789061 34.0 27.0 35.0 2.0 35.0 102-103 26.26374179272478 32.0 24.5 34.0 2.0 35.0 104-105 27.187066211193006 34.0 26.5 35.0 2.0 35.0 106-107 27.360873646102412 34.0 27.0 35.0 2.0 35.0 108-109 27.374129419174984 34.0 27.0 35.0 2.0 35.0 110-111 27.319087168000024 34.0 27.0 35.0 2.0 35.0 112-113 27.275713993401745 34.0 27.0 35.0 2.0 35.0 114-115 27.198989159209027 34.0 27.0 35.0 2.0 35.0 116-117 27.084282314650824 34.0 27.0 35.0 2.0 35.0 118-119 26.982158523893744 34.0 26.5 35.0 2.0 35.0 120-121 26.851874223920216 34.0 25.5 35.0 2.0 35.0 122-123 26.66939411618903 34.0 25.0 35.0 2.0 35.0 124-125 25.87390017406611 33.0 22.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6677268598461481 1101 2 0.6661787125374552 1101 3 0.5350529240045674 1101 4 0.609829975783839 1101 5 0.8652109030462469 1101 6 0.8807102756460168 1101 7 0.8960832592797487 1101 8 0.9367319265897649 1101 9 1.1436292292748078 1101 10-11 1.1097297879893162 1101 12-13 1.0690688397900345 1101 14-15 1.124417626529386 1101 16-17 1.314268622644562 1101 18-19 1.2575709338016523 1101 20-21 1.245562614934279 1101 22-23 1.2305598315988036 1101 24-25 1.1659532637997998 1101 26-27 1.2715469495458223 1101 28-29 1.4084641020492796 1101 30-31 1.360903276241885 1101 32-33 1.3963686952250711 1101 34-35 1.22360729327346 1101 36-37 1.2804424762110145 1101 38-39 1.371224821733243 1101 40-41 1.396421301390582 1101 42-43 1.4491787341389042 1101 44-45 1.343744989774379 1101 46-47 1.4656180849588907 1101 48-49 1.4262393138779785 1101 50-51 1.4018282548827443 1101 52-53 1.4603812880911917 1101 54-55 1.3518395949649928 1101 56-57 1.397439454217622 1101 58-59 1.4242035802541473 1101 60-61 1.4074406452569903 1101 62-63 1.4311312909590796 1101 64-65 1.4890656060481255 1101 66-67 1.4208430158764926 1101 68-69 1.4247733469473047 1101 70-71 1.4563935388857772 1101 72-73 1.4764957327132393 1101 74-75 1.3603358333368298 1101 76-77 1.3613691666565124 1101 78-79 1.3747881910225104 1101 80-81 1.341339713768523 1101 82-83 1.1985954349633836 1101 84-85 1.2317847240750872 1101 86-87 1.2586086842847273 1101 88-89 1.2239944233865536 1101 90-91 1.2950991309934672 1101 92-93 1.2652588937239138 1101 94-95 1.1809236116186739 1101 96-97 1.2703844808858804 1101 98-99 1.3350678340877629 1101 100-101 1.268727582445461 1101 102-103 1.3202816903859684 1101 104-105 1.2757200533051467 1101 106-107 1.2443501466651519 1101 108-109 1.2803305517700636 1101 110-111 1.2063516020068938 1101 112-113 1.1239495762230547 1101 114-115 1.1483690000905042 1101 116-117 1.2134471854846076 1101 118-119 1.2363297788991119 1101 120-121 1.2766254921701226 1101 122-123 1.3572284766782872 1101 124-125 1.2380607683482268 1102 1 0.5812928096130285 1102 2 -0.3965568223442659 1102 3 0.17913366654372354 1102 4 0.4691206675455746 1102 5 0.9145401971040741 1102 6 0.790228355701025 1102 7 0.7607456861395008 1102 8 0.7464369305645846 1102 9 1.0269439418345243 1102 10-11 1.1290859031229914 1102 12-13 1.1094372446343002 1102 14-15 1.174715379861432 1102 16-17 1.3388167107924431 1102 18-19 1.263367186729056 1102 20-21 1.2240151570279707 1102 22-23 1.1995847118170957 1102 24-25 1.230333872412622 1102 26-27 1.262238174148166 1102 28-29 1.4062933495551633 1102 30-31 1.2324360523843723 1102 32-33 1.1986617360690204 1102 34-35 1.051892069358658 1102 36-37 0.9781095081313111 1102 38-39 0.9188229209819596 1102 40-41 0.9249823452997887 1102 42-43 0.7382157536692233 1102 44-45 0.5355086993189531 1102 46-47 0.6728488536387474 1102 48-49 0.7921323232098487 1102 50-51 0.819583878714468 1102 52-53 0.8531356586016159 1102 54-55 0.9381449111958631 1102 56-57 0.7810309717390602 1102 58-59 0.838542829264032 1102 60-61 0.677206238364441 1102 62-63 0.7491216439659709 1102 64-65 0.8070760121958465 1102 66-67 0.7731040075043083 1102 68-69 0.5704723063325332 1102 70-71 0.48913387219762683 1102 72-73 0.5972781274879644 1102 74-75 0.6726917909101147 1102 76-77 0.7077970219014844 1102 78-79 0.6777533701758394 1102 80-81 0.5968253220590647 1102 82-83 0.5362514734654162 1102 84-85 0.5692781888997303 1102 86-87 0.6557511117174251 1102 88-89 0.6593131967072807 1102 90-91 0.553989476122343 1102 92-93 0.7359924089678813 1102 94-95 0.5205604349146924 1102 96-97 0.5373412019108841 1102 98-99 0.5422980656299217 1102 100-101 0.29332992150110115 1102 102-103 0.38801278492936575 1102 104-105 0.45051092769370626 1102 106-107 0.6011639174434258 1102 108-109 0.6848519978879892 1102 110-111 0.4709809053751073 1102 112-113 0.6245440086117426 1102 114-115 0.7330867163242374 1102 116-117 0.7166030485216552 1102 118-119 0.5395873400074791 1102 120-121 0.6962240354526834 1102 122-123 0.6589021977534237 1102 124-125 0.49142199703281264 1103 1 0.07612085665508417 1103 2 -1.1238153754447637 1103 3 -0.21389466560167847 1103 4 -0.007025685507407786 1103 5 0.23088166776218344 1103 6 0.28031210481007207 1103 7 0.2700308488859946 1103 8 0.3667639084690748 1103 9 0.411065764120103 1103 10-11 0.5596795714527616 1103 12-13 0.5070702928642845 1103 14-15 0.5464760415387886 1103 16-17 0.6475465159718183 1103 18-19 0.5716819586463728 1103 20-21 0.6837873713306593 1103 22-23 0.597101194354515 1103 24-25 0.482218969822938 1103 26-27 0.5590960976454369 1103 28-29 0.6631098439719025 1103 30-31 0.586327267637401 1103 32-33 0.6433672777653854 1103 34-35 0.609206009724538 1103 36-37 0.7131735078937282 1103 38-39 0.6972122355459973 1103 40-41 0.6502949195454519 1103 42-43 0.6288652547383649 1103 44-45 0.6463886589293182 1103 46-47 0.5716668626670227 1103 48-49 0.5925703726277689 1103 50-51 0.5345562593274451 1103 52-53 0.5724209698394418 1103 54-55 0.5044841544840537 1103 56-57 0.5412991470776127 1103 58-59 0.48981985468573797 1103 60-61 0.47985680857113877 1103 62-63 0.5436690423979051 1103 64-65 0.5259589119345023 1103 66-67 0.5135010733156626 1103 68-69 0.5338149946195401 1103 70-71 0.5241696586076969 1103 72-73 0.579811092562803 1103 74-75 0.5286871282824102 1103 76-77 0.504496802134323 1103 78-79 0.5154352720527697 1103 80-81 0.5832146069248303 1103 82-83 0.6100932411061848 1103 84-85 0.6116331128721697 1103 86-87 0.529586197248431 1103 88-89 0.5395861237683413 1103 90-91 0.5232376645281427 1103 92-93 0.566077494498888 1103 94-95 0.49491301272414745 1103 96-97 0.532942888772368 1103 98-99 0.5360933554849936 1103 100-101 0.5183217544780554 1103 102-103 0.5602991059082179 1103 104-105 0.5763847991596123 1103 106-107 0.5641286953004006 1103 108-109 0.5441831929461998 1103 110-111 0.6042273712814996 1103 112-113 0.5922590240548402 1103 114-115 0.563181453791568 1103 116-117 0.6460459090585999 1103 118-119 0.7076342335688963 1103 120-121 0.6767545949680063 1103 122-123 0.6924397066629027 1103 124-125 0.6291254301523175 1104 1 0.3148609620040155 1104 2 -0.7420502715509301 1104 3 0.20275053840274637 1104 4 0.2995571676443305 1104 5 0.6913172506735421 1104 6 0.7645672412980531 1104 7 0.7775154976258847 1104 8 0.7645794459814468 1104 9 0.9192815984720042 1104 10-11 0.988207087313306 1104 12-13 0.9554442233689073 1104 14-15 1.1002300066746997 1104 16-17 1.1865667272517157 1104 18-19 1.0930844163030855 1104 20-21 1.2055028564595744 1104 22-23 1.1294983554627152 1104 24-25 1.0860606044774954 1104 26-27 0.9809167134419496 1104 28-29 1.250320825579486 1104 30-31 1.128238936050188 1104 32-33 1.2150436571961478 1104 34-35 1.151740095342376 1104 36-37 1.1269068834248372 1104 38-39 1.1213724634139837 1104 40-41 1.148928402753647 1104 42-43 1.1928243519969683 1104 44-45 1.0433449624040847 1104 46-47 1.0110854064071972 1104 48-49 1.0872247146334715 1104 50-51 1.0010748700020287 1104 52-53 0.7015325247547537 1104 54-55 0.9732805356609333 1104 56-57 1.1035077641546245 1104 58-59 1.0649144359091913 1104 60-61 1.1000517186462275 1104 62-63 1.0258630357626686 1104 64-65 1.0414358559424173 1104 66-67 0.9985335947055702 1104 68-69 0.9034720649297654 1104 70-71 1.0335432035119538 1104 72-73 0.9930826570817501 1104 74-75 0.9834309222990569 1104 76-77 0.9575846971928144 1104 78-79 1.0499114012070656 1104 80-81 0.9988794401708567 1104 82-83 0.9054325694339873 1104 84-85 0.969143771448504 1104 86-87 1.0158713741880732 1104 88-89 0.8948262873211021 1104 90-91 0.8830390075815124 1104 92-93 0.9295129391761456 1104 94-95 0.9242593837153592 1104 96-97 0.915467916419189 1104 98-99 1.0445069009712853 1104 100-101 1.0881792642271968 1104 102-103 1.0277855371403959 1104 104-105 0.9856674005797856 1104 106-107 1.0007392680260843 1104 108-109 0.9006205365234052 1104 110-111 0.8764832425928297 1104 112-113 0.9261416560320228 1104 114-115 1.0278142603802394 1104 116-117 1.0336208464894838 1104 118-119 0.9015477499532736 1104 120-121 0.9114373477181381 1104 122-123 0.8841880405782732 1104 124-125 0.8180437847300475 1105 1 0.5573223668463498 1105 2 0.3796950905058587 1105 3 0.5778050283239224 1105 4 0.462523835760841 1105 5 0.6424899317709745 1105 6 0.5606871860935669 1105 7 0.625275456146575 1105 8 0.636961875821143 1105 9 0.7317130329946906 1105 10-11 0.8687772966360754 1105 12-13 0.7885132710915173 1105 14-15 0.9112454626881927 1105 16-17 0.9271147885013562 1105 18-19 1.004476555256005 1105 20-21 1.042860711465643 1105 22-23 0.8136334863248251 1105 24-25 0.9044352203554809 1105 26-27 0.9378480316320221 1105 28-29 1.081634562562833 1105 30-31 1.0366223407364927 1105 32-33 0.9837129708613261 1105 34-35 1.0101893297909115 1105 36-37 1.01527741903125 1105 38-39 0.9383051762146479 1105 40-41 0.7882335000553624 1105 42-43 0.912747158229628 1105 44-45 0.985345455350604 1105 46-47 1.0027967592968423 1105 48-49 0.9563718895459417 1105 50-51 0.9036406428061525 1105 52-53 0.9073704199145638 1105 54-55 0.8646017773062127 1105 56-57 0.8860534186653872 1105 58-59 0.8386908549772407 1105 60-61 0.9229307541280178 1105 62-63 0.884638726457375 1105 64-65 0.8531644274400314 1105 66-67 0.7665302849218278 1105 68-69 0.7116151409298297 1105 70-71 0.7593261013056392 1105 72-73 0.7487511277669654 1105 74-75 0.7797663068146008 1105 76-77 0.8795148106326494 1105 78-79 0.90395152594877 1105 80-81 0.643149972532381 1105 82-83 0.6670124284954717 1105 84-85 0.6048740399524668 1105 86-87 0.6886863749509686 1105 88-89 0.7334642153440036 1105 90-91 0.6985829091634308 1105 92-93 0.514234531407201 1105 94-95 0.6424868405601032 1105 96-97 0.6842919144915882 1105 98-99 0.7600821467714027 1105 100-101 0.7500659721384366 1105 102-103 0.3202069332284445 1105 104-105 0.27123107525441625 1105 106-107 0.7059455495731122 1105 108-109 0.6853530025746473 1105 110-111 0.6106101576796839 1105 112-113 0.420697876444013 1105 114-115 0.65796039071558 1105 116-117 0.36913322434886453 1105 118-119 0.26848032467367844 1105 120-121 0.2413611688406867 1105 122-123 0.26083787526168933 1105 124-125 0.0981806636882041 1106 1 0.43544074376574926 1106 2 0.38255136753932106 1106 3 0.5119205517896255 1106 4 0.34260255042676846 1106 5 0.43753346764430745 1106 6 0.5869103502650361 1106 7 0.6528444114738363 1106 8 0.6344632216122932 1106 9 0.597538595874191 1106 10-11 0.7441655056233216 1106 12-13 0.7914363671264155 1106 14-15 0.8565446988811303 1106 16-17 1.018745285070061 1106 18-19 0.8880453863563957 1106 20-21 0.9269513895561587 1106 22-23 0.7956154131966002 1106 24-25 0.8693190117597354 1106 26-27 0.8123807691782758 1106 28-29 1.0084428164976273 1106 30-31 0.8123664785455063 1106 32-33 0.7669996563753401 1106 34-35 0.7574850424579083 1106 36-37 0.8735246719232848 1106 38-39 0.880230523391127 1106 40-41 0.9694094580139705 1106 42-43 0.8650388617565383 1106 44-45 0.8801219975519743 1106 46-47 0.75721340118087 1106 48-49 0.869526951038246 1106 50-51 1.0176197425753912 1106 52-53 1.0257205176020605 1106 54-55 0.9944353321147403 1106 56-57 0.8284304999361218 1106 58-59 0.8179628478398406 1106 60-61 0.9409017716214194 1106 62-63 0.9399253329179231 1106 64-65 0.792113505714255 1106 66-67 0.6682861188794291 1106 68-69 0.8384310178774612 1106 70-71 0.8100407822872562 1106 72-73 0.9126971944805611 1106 74-75 0.9013552438466306 1106 76-77 0.8853845305198682 1106 78-79 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-2.3801158692512416 2216 118-119 -2.4527066476957273 2216 120-121 -2.449106705842791 2216 122-123 -2.411967126606946 2216 124-125 -2.593392867175865 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 97224.0 3 31214.0 4 14714.0 5 10988.0 6 11487.0 7 13386.0 8 15136.0 9 16429.0 10 17656.0 11 19016.0 12 21395.0 13 22818.0 14 24087.0 15 20381.0 16 14498.0 17 12582.0 18 11613.0 19 10955.0 20 10648.0 21 11228.0 22 13169.0 23 17510.0 24 23955.0 25 32102.0 26 38407.0 27 43379.0 28 50587.0 29 58639.0 30 67583.0 31 80515.0 32 97930.0 33 121667.0 34 155772.0 35 210800.0 36 332642.0 37 526653.0 38 217615.0 39 947.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.661321244198 19.620298048202955 13.598113710257401 25.120266997341645 2 15.419401452149554 20.387477644518697 39.44218190298994 24.750939000341816 3 19.428532987470206 24.345350048271612 28.952796329835863 27.273320634422326 4 12.853594795321685 16.95841399233843 36.72031741938171 33.46767379295818 5 13.31103092176492 38.32041371614333 34.20207795349739 14.166477408594357 6 32.20713758290901 37.283227272800076 16.877199361234222 13.63243578305669 7 28.366901237563695 32.450509064495385 20.524833361763744 18.657756336177176 8 25.645430352304828 37.1010833187177 18.91903580069242 18.334450528285053 9 25.73253733112349 16.521076853402036 19.747024101504294 37.999361713970174 10-11 24.89479961746044 27.251456458880803 27.01527393766945 20.83846998598931 12-13 25.480394082633367 24.190212569388255 27.986891143913283 22.34250220406509 14-15 22.326318607421296 25.709773716172208 25.908540190693273 26.05536748571322 16-17 22.157439658467286 28.930923437987023 26.421009742624523 22.49062716092117 18-19 21.949356540597595 27.456837255435474 28.81897665158451 21.77482955238242 20-21 22.63480610059478 26.701755819224786 28.926625145971197 21.73681293420924 22-23 22.312697386706137 26.529414073356772 28.82791043018788 22.32997810974921 24-25 22.165459309093283 26.64490873642098 28.94072342148225 22.248908533003487 26-27 22.070037283863908 26.922205221823177 28.811325068763523 22.196432425549396 28-29 21.870171764735577 26.85921549546395 29.052232128280597 22.218380611519876 30-31 22.176811446798915 26.69696439433042 28.909690240805467 22.216533918065196 32-33 22.04843470097069 26.686958414264396 28.88915095614616 22.375455928618752 34-35 22.343769958839992 26.757809449863796 28.741710637013096 22.15670995428312 36-37 22.298174587173357 26.648428488797315 28.75384085895091 22.29955606507842 38-39 22.130541975480185 26.85357183901027 28.814948943410297 22.200937242099254 40-41 22.302445775022655 26.704492443320397 28.698924890492915 22.294136891164033 42-43 22.251763748589443 26.62033014073949 28.602520064166892 22.525386046504178 44-45 22.379708384204392 26.90032182409432 28.344065474805664 22.375904316895625 46-47 22.334960539809163 27.136874746478934 27.919972034098862 22.608192679613044 48-49 22.589470733685044 27.04704033315648 27.87782927732674 22.485659655831743 50-51 22.48702206689101 27.212275098545796 27.69775631117568 22.60294652338751 52-53 22.601908562543635 27.307884898418287 27.52455678066667 22.565649758371407 54-55 22.518436712533042 27.126343486455717 27.678013331854416 22.677206469156822 56-57 22.38436016955176 27.427906364918297 27.705767510828235 22.481965954701703 58-59 22.55423498804922 27.132590165404853 27.729848754288085 22.583326092257842 60-61 22.625871581895364 27.14898769764632 27.82721285598562 22.397927864472695 62-63 22.488945180186658 27.184565737686732 27.79609958968129 22.53038949244532 64-65 22.424517386383407 26.96613781169244 27.84568297092055 22.763661831003596 66-67 22.26346409581124 27.29674568048157 27.691768038386645 22.748022185320547 68-69 22.335319606279885 27.07406418193678 27.81374096176401 22.77687525001932 70-71 22.256837010131232 26.98429160458362 27.855022590153393 22.903848795131754 72-73 22.464099346747563 27.078011279100988 27.696886224116017 22.761003150035428 74-75 22.406995581310277 27.265151616779125 27.503154991313387 22.824697810597208 76-77 22.494877985639338 27.12666456707291 27.585238267518815 22.79321917976894 78-79 22.506466472150272 27.179645093339637 27.611590817219128 22.702297617290963 80-81 22.27951762939969 27.362164168660325 27.63698190619717 22.72133629574281 82-83 22.56450463606894 27.356440633070523 27.44527584665244 22.633778884208095 84-85 22.50581272775652 27.16098027426585 27.506930925793892 22.826276072183738 86-87 22.31906621329984 27.09219159533507 27.776606520081266 22.812135671283826 88-89 22.496782558624705 27.042124256104326 27.6356591009744 22.825434084296564 90-91 22.403213515891192 27.31582608124607 27.716514497300516 22.564445905562227 92-93 22.441465712377823 27.13389977964858 27.7078146164774 22.716819891496197 94-95 22.317080276303507 27.1881519224774 27.622541653460107 22.872226147758983 96-97 22.380436750051658 27.131371006882986 27.56614282075884 22.92204942230651 98-99 22.32808540099892 27.054588133348762 27.486334246452472 23.130992219199847 100-101 22.219688393833547 27.147152606455162 27.65473693989434 22.978422059816946 102-103 22.307066406708305 26.917463219615563 27.674313655061837 23.101156718614295 104-105 22.286219972690617 26.82292146349472 27.77907027962087 23.111788284193793 106-107 22.216582592806606 27.041028604333878 27.68831123065791 23.054077572201606 108-109 22.26516815957415 26.95795064585426 27.445937874732724 23.330943319838866 110-111 22.149897459276527 27.083104336062082 27.57453498287451 23.192463221786884 112-113 22.245067452494535 26.959233777784185 27.595856864320957 23.199841905400323 114-115 22.061169283576703 27.012526050472996 27.600177070137917 23.32612759581238 116-117 22.014016542507427 26.93300086274436 27.641816732595498 23.411165862152714 118-119 22.015386848599217 26.96616795390953 27.509042401429568 23.50940279606169 120-121 21.986516162763202 27.014470825768388 27.575486400723754 23.42352661074466 122-123 22.004231367337905 26.935874583024916 27.493096047874076 23.566798001763104 124-125 21.943825603612577 26.866948854081635 27.499778258434738 23.689447283871047 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 98.0 1 96.0 2 163.0 3 298.5 4 260.5 5 246.0 6 597.5 7 1287.0 8 2041.5 9 2371.5 10 2017.5 11 1523.0 12 1448.5 13 1584.0 14 1826.5 15 2068.0 16 2284.0 17 2505.0 18 2740.5 19 3067.0 20 3492.5 21 4097.0 22 4986.0 23 6221.5 24 7726.5 25 9637.0 26 12787.5 27 16580.0 28 20010.5 29 23998.5 30 28717.0 31 33362.5 32 37289.0 33 41725.0 34 47544.5 35 52969.5 36 59109.5 37 65759.0 38 70547.5 39 73954.0 40 76671.5 41 80213.5 42 84624.5 43 88847.5 44 93549.0 45 96548.0 46 95917.5 47 96093.5 48 99013.0 49 98829.0 50 95483.5 51 92030.0 52 88361.5 53 85496.0 54 81921.0 55 75799.5 56 68147.0 57 61121.0 58 55390.0 59 49115.5 60 41599.0 61 33280.5 62 25919.0 63 20253.0 64 15600.5 65 11725.0 66 8617.5 67 6793.5 68 5288.0 69 3947.0 70 3034.5 71 2284.5 72 1723.0 73 1500.5 74 1183.0 75 830.5 76 600.5 77 364.0 78 228.5 79 142.0 80 90.5 81 62.0 82 37.0 83 22.0 84 14.0 85 7.0 86 6.5 87 5.5 88 2.0 89 2.0 90 2.0 91 0.5 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05694088119016853 2 0.07423937674161214 3 0.0 4 0.007568091803756576 5 0.02947151093949651 6 4.404709515413881E-4 7 4.0042813776489825E-5 8 4.0042813776489825E-5 9 5.605993928708575E-4 10-11 0.05509891175645 12-13 0.009470125458139843 14-15 0.007027513817773964 16-17 0.05145501570278942 18-19 2.2023547577069405E-4 20-21 0.010371088768110864 22-23 0.013013914477359192 24-25 0.0 26-27 0.0 28-29 0.05075426646170085 30-31 0.0 32-33 0.003543789019219349 34-35 0.0 36-37 6.006422066473474E-5 38-39 0.0 40-41 0.0 42-43 0.0035638104261075942 44-45 0.0 46-47 0.0 48-49 8.008562755297965E-5 50-51 4.404709515413881E-4 52-53 1.0010703444122457E-4 54-55 0.0 56-57 0.0014615627028418785 58-59 0.0 60-61 0.0 62-63 0.0 64-65 5.405779859826126E-4 66-67 0.0 68-69 8.008562755297965E-4 70-71 0.0 72-73 0.0021422905370422057 74-75 0.0037440030881017985 76-77 7.407920548650617E-4 78-79 9.009633099710211E-4 80-81 0.0014215198890653888 82-83 2.602782895471839E-4 84-85 2.2023547577069405E-4 86-87 4.60492358429633E-4 88-89 4.0042813776489825E-5 90-91 0.0 92-93 0.007187685072879923 94-95 0.005826229404479269 96-97 0.006206636135355923 98-99 0.0010411131581887355 100-101 3.203425102119186E-4 102-103 1.601712551059593E-4 104-105 6.80727834200327E-4 106-107 0.0047250520256258 108-109 0.005225587197831922 110-111 0.0010611345650769805 112-113 0.003523767612331105 114-115 4.60492358429633E-4 116-117 0.0025427186748071037 118-119 0.0025827614885835935 120-121 0.013514449649565314 122-123 0.0010210917513004906 124-125 0.002022162095712736 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2497327.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.723974803975885 #Duplication Level Percentage of deduplicated Percentage of total 1 78.39900405575764 46.03901138827116 2 12.095704300953965 14.206156692111271 3 4.001030628833076 7.048692655125881 4 1.8294742847208574 4.297360072018778 5 0.9704892510044145 2.849549316175634 6 0.5862343752032526 2.065560760719621 7 0.39075497328158754 1.6062679643861353 8 0.28017693776562425 1.3162482747202913 9 0.21101850074221085 1.1152660608682523 >10 1.1221981604555218 11.957364941192694 >50 0.08095848798942892 3.2921814221304224 >100 0.03185772870946106 3.2151363792691368 >500 6.864466143259833E-4 0.25927953953495886 >1k 2.7457864572892626E-4 0.28979487091239403 >5k 1.3728932286446313E-4 0.44212966256324077 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5957 0.23853504166654987 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5011 0.2006545398339905 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3265 0.13073978698023928 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.0042813776489825E-5 2 4.0042813776489825E-5 0.0 0.0 0.0 4.0042813776489825E-5 3 4.0042813776489825E-5 0.0 0.0 0.0 4.0042813776489825E-5 4 4.0042813776489825E-5 0.0 0.0 0.0 4.0042813776489825E-5 5 4.0042813776489825E-5 0.0 0.0 0.0 4.0042813776489825E-5 6 4.0042813776489825E-5 0.0 0.0 0.0 4.0042813776489825E-5 7 8.008562755297965E-5 0.0 0.0 0.0 4.0042813776489825E-5 8 8.008562755297965E-5 0.0 0.0 0.0 4.0042813776489825E-5 9 8.008562755297965E-5 0.0 0.0 0.0 4.0042813776489825E-5 10-11 1.2012844132946947E-4 0.0 0.0 0.0 4.0042813776489825E-5 12-13 1.2012844132946947E-4 0.0 0.0 0.0 4.0042813776489825E-5 14-15 2.2023547577069402E-4 0.0 0.0 0.0 4.0042813776489825E-5 16-17 2.8029969643542877E-4 0.0 0.0 0.0 8.008562755297965E-5 18-19 3.003211033236737E-4 0.0 0.0 2.0021406888244913E-5 8.008562755297965E-5 20-21 3.203425102119186E-4 0.0 0.0 6.0064220664734735E-5 8.008562755297965E-5 22-23 4.004281377648982E-4 0.0 0.0 1.6017125510595927E-4 8.008562755297965E-5 24-25 5.205565790943677E-4 0.0 0.0 3.003211033236737E-4 1.2012844132946947E-4 26-27 5.205565790943677E-4 0.0 0.0 3.403639171001635E-4 1.2012844132946947E-4 28-29 6.80727834200327E-4 0.0 0.0 7.007492410885719E-4 1.4014984821771439E-4 30-31 7.207706479768168E-4 0.0 0.0 0.002022162095712736 2.002140688824491E-4 32-33 7.207706479768168E-4 0.0 0.0 0.004664987804961064 2.4025688265893894E-4 34-35 8.008562755297964E-4 0.0 0.0 0.007688220245086046 2.4025688265893894E-4 36-37 8.809419030827761E-4 0.0 0.0 0.013674620904671274 2.4025688265893894E-4 38-39 8.809419030827761E-4 0.0 0.0 0.024346030776105813 2.4025688265893894E-4 40-41 8.809419030827761E-4 0.0 0.0 0.03639891772282925 2.4025688265893894E-4 42-43 0.0010010703444122456 0.0 0.0 0.048872254214205824 2.4025688265893894E-4 44-45 0.001121198785741715 0.0 0.0 0.06212642557422396 2.8029969643542877E-4 46-47 0.0012413272270711845 0.0 0.0 0.07958509238077352 2.8029969643542877E-4 48-49 0.0012813700408476744 0.0 0.0 0.0991059640968123 2.8029969643542877E-4 50-51 0.0012813700408476744 0.0 0.0 0.12171013247364082 2.8029969643542877E-4 52-53 0.0012813700408476744 0.0 0.0 0.1432731876922806 3.403639171001635E-4 54-55 0.0012813700408476744 0.0 0.0 0.16739898299261569 4.004281377648982E-4 56-57 0.0012813700408476744 0.0 0.0 0.19590946640147644 4.004281377648982E-4 58-59 0.0012813700408476744 0.0 0.0 0.22762337491245638 4.004281377648982E-4 60-61 0.0012813700408476744 0.0 0.0 0.2638821427870679 4.004281377648982E-4 62-63 0.0012813700408476744 0.0 0.0 0.3008817027165446 4.004281377648982E-4 64-65 0.0012813700408476744 0.0 0.0 0.3385019262595567 4.004281377648982E-4 66-67 0.0014014984821771439 0.0 0.0 0.38196840061393644 4.004281377648982E-4 68-69 0.0014014984821771439 0.0 0.0 0.43538551419177385 4.4047095154138804E-4 70-71 0.0014615627028418785 0.0 0.0 0.49548977767028507 6.006422066473473E-4 72-73 0.0014815841097301234 0.0 0.0 0.5609597781948459 7.207706479768168E-4 74-75 0.0014815841097301234 0.0 0.0 0.6274308490638191 7.207706479768168E-4 76-77 0.0014815841097301234 0.0 0.0 0.7010295407850073 7.407920548650617E-4 78-79 0.0014815841097301234 2.0021406888244913E-5 0.0 0.7887833671761848 7.608134617533066E-4 80-81 0.0018820122474950216 4.0042813776489825E-5 0.0 0.8918535698368696 7.608134617533066E-4 82-83 0.0018820122474950216 4.0042813776489825E-5 0.0 1.0023116716393168 7.608134617533066E-4 84-85 0.001982119281936246 4.0042813776489825E-5 0.0 1.1152324064890182 7.608134617533066E-4 86-87 0.002062204909489226 4.0042813776489825E-5 0.0 1.234900355460058 7.608134617533066E-4 88-89 0.0020822263163774706 4.0042813776489825E-5 0.0 1.3742693688091308 7.608134617533066E-4 90-91 0.0022223761645951854 4.0042813776489825E-5 0.0 1.5332793823155717 7.608134617533066E-4 92-93 0.00224239757148343 4.0042813776489825E-5 0.0 1.7074055580226377 7.608134617533066E-4 94-95 0.00224239757148343 4.0042813776489825E-5 0.0 1.8828331251774397 7.608134617533066E-4 96-97 0.00224239757148343 4.0042813776489825E-5 0.0 2.0649478422329155 7.608134617533066E-4 98-99 0.0024025688265893893 4.0042813776489825E-5 0.0 2.263560198564305 7.608134617533066E-4 100-101 0.0026027828954718387 4.0042813776489825E-5 0.0 2.499952949693813 7.608134617533066E-4 102-103 0.002782975557466043 4.0042813776489825E-5 0.0 2.762974171984686 8.008562755297964E-4 104-105 0.0034436819847781246 4.0042813776489825E-5 0.0 3.0278774065230545 8.208776824180414E-4 106-107 0.0036639174605488187 4.0042813776489825E-5 0.0 3.2906783933381574 8.809419030827761E-4 108-109 0.0037640244949900432 4.0042813776489825E-5 0.0 3.572359566848875 8.809419030827761E-4 110-111 0.0037640244949900432 4.0042813776489825E-5 0.0 3.900210104643885 8.809419030827761E-4 112-113 0.0037640244949900432 4.0042813776489825E-5 0.0 4.261336220687159 8.809419030827761E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5060 0.0 50.03263 1 GTATCAA 8085 0.0 45.827408 1 ATCAACG 9795 0.0 36.74958 3 TCAACGC 9935 0.0 36.23535 4 TATCAAC 10335 0.0 35.259247 2 CAACGCA 10085 0.0 35.13734 5 AACGCAG 10215 0.0 34.56594 6 GTACATG 9320 0.0 30.03979 1 TACATGG 9180 0.0 29.64187 2 ACGCAGA 12110 0.0 29.009481 7 ACATGGG 9190 0.0 28.939665 3 CGCAGAG 12210 0.0 28.723125 8 GCAGAGT 13695 0.0 24.9569 9 GAGTACT 8360 0.0 23.687172 12-13 TCGCGGA 315 0.0 23.605068 82-83 AGTACTT 8580 0.0 22.802156 12-13 GTACTTT 8845 0.0 22.15266 14-15 CGCGATC 365 0.0 22.02503 22-23 CAGAGTA 13575 0.0 21.76796 10-11 AGAGTAC 13300 0.0 21.344557 10-11 >>END_MODULE