FastQCFastQC Report
Thu 26 May 2016
SRR1364596_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364596_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2020130
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA86400.4276952473355676No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26840.13286273655655823No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA93600.032.521531
GTACATG114250.031.3881841
TACATGG115400.030.0946042
ACATGGG119950.028.953043
TCAACGC111050.027.5714554
ATCAACG111650.027.4773223
CAACGCA112300.027.1591455
AACGCAG111850.026.4452326
CGCATAG2057.778726E-826.120798
TATCAAC116750.026.0729332
GGTATCA67050.025.5870131
CATGGGA87200.025.138914
GAGTACT38100.023.50048812-13
ACGCAGA126800.022.9020147
CGCAGAG124350.022.248718
CGCAGAT5950.021.9990258
CATGGGG35550.021.6154964
TATAGCG1400.003135095821.2749182
GTACTTT43600.021.21902514-15
TACCTGG19800.020.7591612
Produced by FastQC (version 0.11.5)