##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364596_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2020130 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.032555330597535 31.0 26.0 31.0 7.0 34.0 2 25.5261691079287 30.0 25.0 31.0 7.0 34.0 3 26.27166865498755 31.0 26.0 31.0 7.0 34.0 4 29.541803250285874 35.0 28.0 37.0 7.0 37.0 5 29.930630206966878 35.0 30.0 37.0 7.0 37.0 6 29.829548593407356 35.0 30.0 37.0 2.0 37.0 7 29.718385450441307 35.0 30.0 37.0 2.0 37.0 8 29.521880770049453 35.0 30.0 37.0 2.0 37.0 9 30.426926979946835 37.0 29.0 39.0 2.0 39.0 10-11 30.395937142659136 37.0 29.0 39.0 2.0 39.0 12-13 30.385623697484817 37.0 29.0 39.0 2.0 39.0 14-15 30.96180988352235 37.0 28.0 40.0 2.0 41.0 16-17 31.342733883462945 37.5 30.0 40.0 2.0 41.0 18-19 31.148702806255045 37.0 28.5 40.0 2.0 41.0 20-21 31.058419260146625 37.0 28.0 40.0 2.0 41.0 22-23 31.10352526817581 37.0 28.5 40.0 2.0 41.0 24-25 31.191745580729954 37.0 29.0 40.0 2.0 41.0 26-27 31.008960561944033 37.0 28.5 40.0 2.0 41.0 28-29 30.89084514362937 37.0 28.0 40.0 2.0 41.0 30-31 30.74738506927772 37.0 28.0 40.0 2.0 41.0 32-33 30.65314608465792 37.0 28.0 40.0 2.0 41.0 34-35 30.691095622558944 37.0 28.5 40.0 2.0 41.0 36-37 30.609830555459304 37.0 28.0 40.0 2.0 41.0 38-39 30.302694133545863 37.0 27.0 40.0 2.0 41.0 40-41 30.165994267695645 37.0 27.0 40.0 2.0 41.0 42-43 29.643803121581286 36.0 25.5 40.0 2.0 41.0 44-45 29.45725794874587 36.0 25.5 40.0 2.0 41.0 46-47 29.390141228534794 35.5 25.5 40.0 2.0 41.0 48-49 29.297759302619138 36.0 26.0 40.0 2.0 41.0 50-51 29.058650680896775 35.0 25.0 40.0 2.0 41.0 52-53 28.957866572943324 35.0 25.0 40.0 2.0 41.0 54-55 28.791089929856 35.0 24.5 39.0 2.0 41.0 56-57 28.576010702281536 35.0 24.0 39.0 2.0 40.5 58-59 28.378706815897985 35.0 24.0 39.0 2.0 40.0 60-61 28.086683530267855 34.0 23.0 39.0 2.0 40.0 62-63 27.901627618024584 34.0 23.0 39.0 2.0 40.0 64-65 27.677476449535426 34.0 22.5 38.0 2.0 40.0 66-67 27.416734319078476 34.0 22.0 38.0 2.0 40.0 68-69 27.181722710914645 34.0 22.0 37.5 2.0 40.0 70-71 26.749234702717153 33.0 20.0 37.0 2.0 39.5 72-73 26.50513778816215 33.0 20.0 36.5 2.0 39.0 74-75 26.483193903362654 33.0 20.5 36.0 2.0 39.0 76-77 26.237439669724225 33.0 20.0 36.0 2.0 39.0 78-79 25.950121031814785 33.0 19.5 35.5 2.0 37.5 80-81 25.553271819140353 32.0 18.0 35.0 2.0 37.0 82-83 25.665176993559818 33.0 19.0 35.0 2.0 37.0 84-85 25.50038834134437 33.0 18.0 35.0 2.0 36.0 86-87 25.372490136773376 33.0 18.0 35.0 2.0 36.0 88-89 25.201926856192422 33.0 17.5 35.0 2.0 36.0 90-91 25.064369619776947 33.0 16.0 35.0 2.0 35.5 92-93 24.93794632028632 33.0 12.5 35.0 2.0 35.0 94-95 24.831008152940655 32.5 10.5 35.0 2.0 35.0 96-97 24.714006524332593 32.0 7.5 35.0 2.0 35.0 98-99 24.617046180196326 32.0 7.0 35.0 2.0 35.0 100-101 24.507679951290264 32.0 6.0 35.0 2.0 35.0 102-103 23.395990357056228 30.0 2.0 34.0 2.0 35.0 104-105 24.215253226277518 32.0 2.0 34.5 2.0 35.0 106-107 24.36442209164757 32.0 2.0 35.0 2.0 35.0 108-109 24.319303955686017 32.0 2.0 35.0 2.0 35.0 110-111 24.250806136238758 32.0 2.0 35.0 2.0 35.0 112-113 24.157007222307477 32.0 2.0 35.0 2.0 35.0 114-115 24.04724943444234 32.0 2.0 35.0 2.0 35.0 116-117 23.932908030671292 32.0 2.0 35.0 2.0 35.0 118-119 23.80530955928579 32.0 2.0 35.0 2.0 35.0 120-121 23.612498453069854 32.0 2.0 35.0 2.0 35.0 122-123 23.391768599050557 32.0 2.0 35.0 2.0 35.0 124-125 22.46318999272324 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.5424306978015032 1101 2 1.8227331189265676 1101 3 1.3647823913273314 1101 4 1.4298596173308518 1101 5 1.3880131669355933 1101 6 1.1061864710213136 1101 7 1.1609725389863357 1101 8 1.4240699974878517 1101 9 1.643855441045151 1101 10-11 1.6942312252627119 1101 12-13 1.6597507658633752 1101 14-15 1.9588638780637133 1101 16-17 1.5925937433854926 1101 18-19 1.566742874105099 1101 20-21 1.7113418145996881 1101 22-23 1.535571311542732 1101 24-25 1.541155122264037 1101 26-27 1.7611054797537733 1101 28-29 1.701115143318237 1101 30-31 1.873121701205264 1101 32-33 1.8597477156109363 1101 34-35 1.5531665103012564 1101 36-37 1.4307524348739662 1101 38-39 1.4700936749644242 1101 40-41 1.6233930964317693 1101 42-43 1.6092410996582238 1101 44-45 1.438755492988271 1101 46-47 1.009616072492257 1101 48-49 0.9088951260196332 1101 50-51 1.033407714087776 1101 52-53 0.8888886572830756 1101 54-55 0.8493056997701771 1101 56-57 0.995442782199838 1101 58-59 0.8414215618149612 1101 60-61 0.8692672183104619 1101 62-63 0.9194989635192243 1101 64-65 0.8528777023887812 1101 66-67 0.8601452425122282 1101 68-69 0.7112488808458899 1101 70-71 0.6266930403873232 1101 72-73 0.5028846485048355 1101 74-75 0.5604618727085615 1101 76-77 0.7463007065780403 1101 78-79 0.7466900173991391 1101 80-81 0.8282426168418837 1101 82-83 0.8490776835749649 1101 84-85 0.781468803368071 1101 86-87 0.6828951059160424 1101 88-89 0.6648029384063179 1101 90-91 0.6383331149203322 1101 92-93 0.652464378227112 1101 94-95 0.5802372700239573 1101 96-97 0.5915236682215621 1101 98-99 0.7181138682649149 1101 100-101 0.7297239430729441 1101 102-103 0.5624965180521393 1101 104-105 0.7245448327101869 1101 106-107 0.6895040725453185 1101 108-109 0.6773084422089717 1101 110-111 0.7462727788633607 1101 112-113 0.7047500024964144 1101 114-115 0.6329946604986496 1101 116-117 0.5493094309272237 1101 118-119 0.4514183382217922 1101 120-121 0.4542421903691398 1101 122-123 0.4292421301887508 1101 124-125 0.40087546081988634 1102 1 0.6813258389300785 1102 2 1.1819934172285365 1102 3 0.7974732485867193 1102 4 0.735310107685077 1102 5 0.6672181338155063 1102 6 0.3582369598649322 1102 7 0.39901645236805905 1102 8 0.3851799276869592 1102 9 1.1299773751847866 1102 10-11 1.0150437922285178 1102 12-13 1.1881514417526695 1102 14-15 1.2942584003824145 1102 16-17 0.9670894969578114 1102 18-19 0.8633081825551194 1102 20-21 1.1075800763643606 1102 22-23 1.1444177571354786 1102 24-25 0.827704342736233 1102 26-27 1.226763581631353 1102 28-29 0.733246643924101 1102 30-31 0.8719236270149793 1102 32-33 1.1821650340870775 1102 34-35 0.9952426147447113 1102 36-37 0.7401589924243694 1102 38-39 1.0273163245928743 1102 40-41 1.0692172357357492 1102 42-43 1.2441194255590062 1102 44-45 0.7762047662238523 1102 46-47 0.5930562993424573 1102 48-49 0.4823586486070006 1102 50-51 0.5256679508864046 1102 52-53 0.5604519965473429 1102 54-55 0.5520273302775607 1102 56-57 0.6572573032453093 1102 58-59 0.5898364482118232 1102 60-61 0.5164799842449526 1102 62-63 0.4010667374977004 1102 64-65 0.25480917688269855 1102 66-67 0.3096350345770027 1102 68-69 0.23925113381018903 1102 70-71 0.48068611748552925 1102 72-73 0.18825133247156245 1102 74-75 0.3031915310197668 1102 76-77 0.12290386655468666 1102 78-79 0.19712383171733094 1102 80-81 0.3141307843553882 1102 82-83 0.20498561847638186 1102 84-85 0.13825035538856412 1102 86-87 0.2509386200655861 1102 88-89 0.13596844819883458 1102 90-91 0.29747785169145047 1102 92-93 0.41602705066573975 1102 94-95 0.2328423678951843 1102 96-97 0.039394681699420175 1102 98-99 0.21476441818026615 1102 100-101 0.19897648332256068 1102 102-103 0.24036945216048622 1102 104-105 0.3199991966955622 1102 106-107 0.34051332070480456 1102 108-109 0.4191665335122927 1102 110-111 0.28543351729183186 1102 112-113 0.082969027744074 1102 114-115 0.11387597092972257 1102 116-117 0.2448564275682763 1102 118-119 0.3546956567626758 1102 120-121 0.3402421968431746 1102 122-123 0.29048083974086225 1102 124-125 0.19698769469657051 1103 1 1.1689679829291144 1103 2 1.4165418959268656 1103 3 1.126432058606067 1103 4 0.8602529053633745 1103 5 1.027310734786763 1103 6 1.0006517811911664 1103 7 1.0493512636973499 1103 8 1.3071800914299523 1103 9 1.7543503041591073 1103 10-11 1.8452362764251617 1103 12-13 1.7826199449262639 1103 14-15 2.0555048508221496 1103 16-17 1.6999187819611095 1103 18-19 1.5862248761532065 1103 20-21 1.8884673910223775 1103 22-23 1.6910106101847546 1103 24-25 1.6904809283501123 1103 26-27 1.8713088931954829 1103 28-29 1.8309033955216698 1103 30-31 1.979824980480089 1103 32-33 1.9831622504562034 1103 34-35 1.7052100385072642 1103 36-37 1.598870873702083 1103 38-39 1.6757638902085645 1103 40-41 1.7356736219279085 1103 42-43 1.9712666132731123 1103 44-45 1.719939765426684 1103 46-47 1.574848617210698 1103 48-49 1.379067845361444 1103 50-51 1.3727678685840061 1103 52-53 1.2721288109705142 1103 54-55 1.4699566493221603 1103 56-57 1.442054520476244 1103 58-59 1.3764661865246666 1103 60-61 1.5177401589328738 1103 62-63 1.2561025885949952 1103 64-65 1.0869818740314159 1103 66-67 0.9962576629967543 1103 68-69 1.1021681936754852 1103 70-71 1.2456280964488649 1103 72-73 1.0593412144473362 1103 74-75 1.1481581576124888 1103 76-77 1.052627528709806 1103 78-79 0.9865346154860895 1103 80-81 1.1010244505178974 1103 82-83 1.156325402421814 1103 84-85 1.1186083061881043 1103 86-87 1.06013408179928 1103 88-89 1.045766204058033 1103 90-91 1.1058107541221744 1103 92-93 1.170069886694371 1103 94-95 1.0991088337612354 1103 96-97 1.0457147303052352 1103 98-99 1.0004072817866643 1103 100-101 0.9482677562519655 1103 102-103 0.8171677164847679 1103 104-105 0.9560689770645041 1103 106-107 1.0576820966397555 1103 108-109 1.0660095255276687 1103 110-111 1.041417569586173 1103 112-113 1.1093901445574623 1103 114-115 1.1554820957794476 1103 116-117 1.1562546634529838 1103 118-119 1.1182338627427413 1103 120-121 1.0597432331639567 1103 122-123 0.9166284411214782 1103 124-125 0.7888080858699631 1104 1 1.030942815445595 1104 2 1.4036852322317337 1104 3 0.7883174900586631 1104 4 0.9887372831332435 1104 5 0.424026042774063 1104 6 0.6283189873250201 1104 7 0.35295683028456537 1104 8 0.6630524404843534 1104 9 1.10610044795326 1104 10-11 1.08088555643414 1104 12-13 1.1058012836976339 1104 14-15 1.0974976847083404 1104 16-17 0.8504612143077139 1104 18-19 0.8845527829312267 1104 20-21 1.2085461557827948 1104 22-23 1.0557290095868836 1104 24-25 0.979038963159141 1104 26-27 1.2212310020403194 1104 28-29 1.1922016622123088 1104 30-31 1.2382123880660387 1104 32-33 1.063412618071851 1104 34-35 1.1202420430678721 1104 36-37 0.980286133354145 1104 38-39 1.0676987600668895 1104 40-41 1.044032366333962 1104 42-43 1.201890909446977 1104 44-45 1.0009433465705833 1104 46-47 0.7533747941227773 1104 48-49 0.6416152400409949 1104 50-51 0.648882767443471 1104 52-53 0.5338867725752436 1104 54-55 0.6088303935943102 1104 56-57 0.5584833050681404 1104 58-59 0.5307579477256255 1104 60-61 0.5042435953261162 1104 62-63 0.5689321832804133 1104 64-65 0.574322249770951 1104 66-67 0.6334602386656343 1104 68-69 0.3625655310286824 1104 70-71 0.49888052245704984 1104 72-73 0.4235246103359067 1104 74-75 0.29423771053065195 1104 76-77 0.49042173281715407 1104 78-79 0.4323956395555122 1104 80-81 0.45549552398108517 1104 82-83 0.47009013733681826 1104 84-85 0.4524179775722388 1104 86-87 0.45625859031661165 1104 88-89 0.3557278865835052 1104 90-91 0.3862761434121573 1104 92-93 0.35556035546967735 1104 94-95 0.5007815327678777 1104 96-97 0.3788519807060098 1104 98-99 0.4568486539463308 1104 100-101 0.4082427660401713 1104 102-103 0.36329184240125656 1104 104-105 0.3274899055857361 1104 106-107 0.43654381449203683 1104 108-109 0.4159439094829196 1104 110-111 0.38712214179379245 1104 112-113 0.2948525559375419 1104 114-115 0.2993771572952859 1104 116-117 0.2624241658295752 1104 118-119 0.2948562816217972 1104 120-121 0.3566528067298087 1104 122-123 0.45836158468479127 1104 124-125 0.28076677263082317 1105 1 0.8664284205934187 1105 2 1.1334746088178171 1105 3 0.6952395504146018 1105 4 0.6968237987107244 1105 5 0.6227581391753603 1105 6 0.5465767236059413 1105 7 0.4621376744149295 1105 8 0.6380916900156883 1105 9 0.7068821441793034 1105 10-11 0.6219828898161524 1105 12-13 0.62577411223646 1105 14-15 0.8946407154142513 1105 16-17 0.5733599952116037 1105 18-19 0.6934209947347547 1105 20-21 0.5579479282962474 1105 22-23 0.6651721992213986 1105 24-25 0.4493456068111641 1105 26-27 0.8593148086552276 1105 28-29 0.9093106388875363 1105 30-31 0.8891768291027731 1105 32-33 0.9541759579813949 1105 34-35 0.7098951174990837 1105 36-37 0.3154500623109193 1105 38-39 0.4766821814677371 1105 40-41 0.7358957422930636 1105 42-43 0.823031162364618 1105 44-45 0.6508395729500442 1105 46-47 0.26972955239140717 1105 48-49 0.26360511259175823 1105 50-51 0.4195410201689107 1105 52-53 0.26043593825254163 1105 54-55 0.3073043411801848 1105 56-57 0.3722530919309115 1105 58-59 0.5741996038900794 1105 60-61 0.490430771420435 1105 62-63 0.3504433353512084 1105 64-65 0.1497072120280336 1105 66-67 0.0038508580591205543 1105 68-69 -0.08121064319433557 1105 70-71 0.15395341684192232 1105 72-73 0.1525352388697847 1105 74-75 0.18661362808418502 1105 76-77 0.22626462305989392 1105 78-79 0.26769727236119323 1105 80-81 0.3585987549697691 1105 82-83 0.3719546631886317 1105 84-85 0.2608683040956059 1105 86-87 0.39965087243817266 1105 88-89 0.5390079324617076 1105 90-91 0.2775166468301471 1105 92-93 0.3962731147883183 1105 94-95 0.285518679464392 1105 96-97 0.16803683057576535 1105 98-99 0.2507118150349683 1105 100-101 0.260262380523951 1105 102-103 0.09567634637992839 1105 104-105 0.2891044200015713 1105 106-107 0.3372791163241864 1105 108-109 0.3281686434233464 1105 110-111 0.2968613868346708 1105 112-113 0.18064365904137247 1105 114-115 0.3313497352917949 1105 116-117 0.34952449942436203 1105 118-119 0.2390725311166193 1105 120-121 0.09966054626316279 1105 122-123 0.022198098898346785 1105 124-125 0.09612448159129983 1106 1 0.3622953665673698 1106 2 -2.248746924687744 1106 3 -0.15879374023840853 1106 4 0.512822586167232 1106 5 0.6557411318955779 1106 6 0.5477575961848125 1106 7 0.857139552047574 1106 8 0.7493120155021771 1106 9 1.2491910894021991 1106 10-11 1.3901699110763843 1106 12-13 1.393613319015845 1106 14-15 1.4296885384914866 1106 16-17 1.096556110548022 1106 18-19 1.0888312009123915 1106 20-21 1.3032261346367626 1106 22-23 0.9431359179294034 1106 24-25 0.9972557790556849 1106 26-27 0.9745002192233585 1106 28-29 0.9996745376672997 1106 30-31 1.196313416697727 1106 32-33 1.377174921890127 1106 34-35 1.0767884951113658 1106 36-37 0.861637563159249 1106 38-39 1.0951342203958134 1106 40-41 1.2106585497836875 1106 42-43 1.3593565674129628 1106 44-45 1.1366583565155572 1106 46-47 0.9574492693439396 1106 48-49 0.7493339733890529 1106 50-51 0.6762569501852411 1106 52-53 0.6472265663772134 1106 54-55 0.5928634232666639 1106 56-57 0.4715057715162949 1106 58-59 0.5342348717128864 1106 60-61 0.6044601936977543 1106 62-63 0.516312950730601 1106 64-65 0.5434801451616877 1106 66-67 0.4999270582735811 1106 68-69 0.47531797599914327 1106 70-71 0.4718298369429945 1106 72-73 0.2896697226112934 1106 74-75 0.28587026653357483 1106 76-77 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-1.9982448081781428 2216 118-119 -2.014170043999542 2216 120-121 -2.1880265230508122 2216 122-123 -2.0148699443499716 2216 124-125 -1.9336235633029126 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 204534.0 3 27023.0 4 17450.0 5 14184.0 6 12738.0 7 13804.0 8 14956.0 9 15714.0 10 16852.0 11 17949.0 12 19508.0 13 20387.0 14 19976.0 15 16525.0 16 13079.0 17 11376.0 18 10315.0 19 9663.0 20 9560.0 21 10203.0 22 12623.0 23 17977.0 24 26521.0 25 32952.0 26 37774.0 27 40269.0 28 46335.0 29 52434.0 30 60382.0 31 70372.0 32 85012.0 33 106449.0 34 138558.0 35 188451.0 36 263319.0 37 279668.0 38 65169.0 39 69.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.761344869650884 19.611376048664063 13.718517754178409 22.908761327506635 2 13.306220083821133 21.51146026932936 41.06029513574408 24.12202451110543 3 18.889841120725013 27.255606058048187 28.26614140543461 25.588411415792194 4 11.260095944048029 17.93442780838396 37.13887560883464 33.66660063873337 5 12.82091521704906 39.973539626247224 33.29572199126232 13.9098231654414 6 30.112646228718187 38.04983387385528 17.826704827383622 14.010815070042911 7 27.59123855812391 34.47301009718121 20.413363744118744 17.52238760057613 8 25.27547153712944 37.318090566677924 19.61459531615298 17.791842580039656 9 25.88986313003242 18.750967296451062 19.794605759729514 35.564563813787 10-11 23.553182670913138 29.482912652549665 26.93889783485569 20.025006841681506 12-13 24.675918464502402 26.781995938597948 27.22917167729954 21.312913919600106 14-15 21.848093267414733 27.7372702636215 25.550856452457666 24.8637800165061 16-17 21.52537120274897 31.21184774551197 25.737434641292573 21.525346410446485 18-19 21.38401057203089 29.536144457309277 28.250226222911834 20.829618747748 20-21 22.588335533683207 28.347267780430542 28.07266213239196 20.99173455349429 22-23 22.163451486322824 28.30951845337999 27.713987305620257 21.813042754676932 24-25 21.95080093377587 28.267651332475747 28.004447165849378 21.777100567899012 26-27 21.92185746818872 28.420187316974406 27.991049517961258 21.66690569687562 28-29 21.62398859008615 28.346455130989856 28.312386064512037 21.71717021441196 30-31 21.702161732956156 28.199730926829897 28.28468704936008 21.813420290853866 32-33 21.557814081399684 28.234397517798477 28.109082152368902 22.098706248432936 34-35 22.12130579651907 28.114730060405247 27.963859480639307 21.80010466243637 36-37 22.121368523134954 27.946010506963987 27.888497869057606 22.044123100843453 38-39 21.93091545270477 28.09049755802405 28.174165766708537 21.80442122256264 40-41 22.026475095794943 27.982491147230178 28.285632011000576 21.705401745974306 42-43 21.738745200624447 27.960125782729445 28.223554727152244 22.077574289493864 44-45 21.857670131793157 28.309197453694868 27.76850184645306 22.064630568058917 46-47 21.91568196963206 28.376529106573372 27.35439771843813 22.353391205356434 48-49 22.11210329979108 28.27334460691503 27.35833503362613 22.256217059667758 50-51 22.196911339542655 28.408198643377457 27.26040741320162 22.134482603878265 52-53 22.317371470401813 28.435143276863727 27.09487052064518 22.152614732089276 54-55 21.993379782301457 28.398725546105975 27.28377674741514 22.32411792417743 56-57 22.153566089771672 28.724223267064918 27.060136474208825 22.062074168954585 58-59 22.251411882494292 28.21407616419109 27.202587507216506 22.331924446098103 60-61 22.279383108672953 28.267715247057 27.411962979380434 22.040938664889616 62-63 22.20381787930409 28.373241726969674 27.31133703116851 22.111603362557734 64-65 22.085057429666413 28.158867137023858 27.248311267626928 22.507764165682797 66-67 22.112407370827242 28.619344195742457 27.020418259177536 22.247830174252766 68-69 22.13596910948866 28.467765501580438 27.121869947655764 22.27439544127514 70-71 21.996673235757832 28.45821009574547 27.28434960916345 22.260767059333244 72-73 22.30313735285605 28.352939310678423 27.028718098160727 22.315205238304806 74-75 22.16749073649367 28.522975948956486 26.96226129419231 22.347272020357536 76-77 22.331623940097707 28.366889160430976 27.10732494417001 22.194161955301308 78-79 22.353547148700176 28.421732059638806 27.041535532463325 22.183185259197696 80-81 22.03684533052516 28.73346779231442 27.14420428245905 22.085482594701364 82-83 22.241173122423035 28.691690448944623 26.855488698542523 22.21164773008982 84-85 22.13463375071913 28.370014155770985 27.047957848471533 22.447394245038353 86-87 22.241310529514163 28.31990471328572 27.170498804825126 22.26828595237499 88-89 22.420826028866806 28.153701099122326 27.118047368892107 22.307425503118765 90-91 22.137169743032935 28.485218072474332 27.273982518505296 22.103629665987437 92-93 22.192097650217278 28.396277630832007 27.162412400625684 22.249212318325032 94-95 22.065683930949483 28.365478905762053 27.094813673536127 22.474023489752334 96-97 22.211245038264646 28.32639870052179 27.022532371054382 22.439823890159186 98-99 22.110400283207323 28.25372341770552 26.882408967835243 22.753467331251915 100-101 21.960861100405122 28.380002503342954 27.228018604051694 22.43111779220023 102-103 22.196448978772075 28.25662149888533 27.165260323671713 22.381669198670878 104-105 22.077721224330364 28.115749066603748 27.33872023095485 22.46780947811104 106-107 21.949859274323977 28.47618445659812 27.22282742731652 22.351128841761383 108-109 22.0361263985178 28.61995888451248 26.739072826668696 22.60484189030102 110-111 21.758657351530726 28.565901943241776 27.126078993608445 22.549361711619053 112-113 22.038930465381377 28.487807900084256 27.02780393517371 22.445457699360656 114-115 21.70115416486597 28.694363385497407 27.02960149395164 22.574880955684986 116-117 21.681048155270513 28.563916258599154 27.17471897860867 22.58031660752166 118-119 21.893118294063196 28.81776303922521 26.76923747812177 22.519881188589824 120-121 21.75551346483312 28.680943101959617 26.95072536299119 22.61281807021608 122-123 21.7905298239075 28.6922029207326 26.84226021721115 22.67500703814875 124-125 21.776605127297753 28.649620610893408 26.89904574699843 22.674728514810415 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 9542.0 1 7214.5 2 3794.0 3 2183.0 4 1388.5 5 1074.5 6 1022.0 7 1213.5 8 1615.5 9 1731.5 10 1581.5 11 1515.0 12 1535.0 13 1612.0 14 1782.0 15 1934.0 16 2092.0 17 2246.5 18 2450.0 19 2739.5 20 3206.5 21 3859.5 22 4613.0 23 5675.5 24 7019.5 25 8773.0 26 11314.5 27 14200.5 28 16997.5 29 20302.5 30 24202.0 31 28344.0 32 32519.5 33 37364.0 34 42943.0 35 47189.5 36 51413.0 37 56841.5 38 60619.5 39 62306.5 40 63522.5 41 65843.0 42 68737.0 43 71236.0 44 73490.5 45 74240.0 46 73265.0 47 73664.5 48 79369.5 49 81210.0 50 77307.0 51 72820.0 52 67743.0 53 65240.5 54 62053.0 55 56241.0 56 49854.5 57 44535.0 58 39955.0 59 34999.5 60 29696.0 61 24265.0 62 19222.0 63 14695.5 64 11239.5 65 9005.5 66 7008.0 67 5479.5 68 4264.0 69 3125.0 70 2361.5 71 1885.0 72 1642.0 73 1435.0 74 1088.5 75 859.0 76 667.5 77 439.5 78 313.0 79 241.5 80 178.0 81 128.5 82 90.5 83 64.5 84 48.5 85 37.5 86 29.5 87 27.0 88 23.0 89 14.5 90 7.5 91 6.0 92 5.0 93 3.0 94 1.5 95 1.0 96 1.0 97 0.5 98 0.0 99 0.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21538217837465903 2 0.25369654428180366 3 0.30433684960868856 4 0.28864479018677014 5 0.32438506432754327 6 0.23731146015355448 7 0.22854964779494388 8 0.2090459524882062 9 0.20800641542871004 10-11 0.15256443892224758 12-13 0.17746382658541776 14-15 0.1933538930662878 16-17 0.16706845599045606 18-19 0.19139857335914023 20-21 0.18731467776826244 22-23 0.20783315925212734 24-25 0.19872483454035136 26-27 0.2496373995732948 28-29 0.18008742011652715 30-31 0.15872740863211773 32-33 0.19882383806982717 34-35 0.15689584333681497 36-37 0.15751461539603887 38-39 0.1897155133580512 40-41 0.20966472454743013 42-43 0.27448728547172707 44-45 0.17627578423170784 46-47 0.16167276363402355 48-49 0.1643706098122398 50-51 0.16981580393341023 52-53 0.18929474835777896 54-55 0.1772163177617282 56-57 0.17615202981986308 58-59 0.15115363862721706 60-61 0.16375183775301588 62-63 0.15315846009910253 64-65 0.14902506274348679 66-67 0.13585759332320196 68-69 0.13917421156064214 70-71 0.14130278744437239 72-73 0.1177151965467569 74-75 0.11882898625335994 76-77 0.14083252067936222 78-79 0.14620346215342578 80-81 0.15632657304232897 82-83 0.15949468598555538 84-85 0.1451144233291917 86-87 0.17224139040556796 88-89 0.16753872275546625 90-91 0.15543554127704654 92-93 0.15518803245335697 94-95 0.17565701217248395 96-97 0.1643706098122398 98-99 0.16003920539767244 100-101 0.13996623979644873 102-103 0.17877562335097244 104-105 0.1369218812650671 106-107 0.14392638097548177 108-109 0.15486627098256053 110-111 0.12994213243702138 112-113 0.12028928831312836 114-115 0.1185072247825635 116-117 0.12325939419740314 118-119 0.10603278006860944 120-121 0.0991272838876706 122-123 0.12642750714062956 124-125 0.12269012390291713 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2020130.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.63879996957369 #Duplication Level Percentage of deduplicated Percentage of total 1 81.20200629551627 50.86396229472907 2 10.41555108078258 13.04835241444034 3 3.5544603121652396 6.679413854805206 4 1.6438420757195502 4.118731798502628 5 0.9268849917296725 2.902948179587746 6 0.5267471360571438 1.9796885094017551 7 0.3600661850786463 1.5787879620064176 8 0.2401202785704878 1.2032676878412074 9 0.1723055827053374 0.9713713435848419 >10 0.8713740958471239 9.57108994994782 >50 0.05611233599023904 2.404872710186066 >100 0.028555585904915744 3.104147907888165 >500 0.0012337774580327319 0.5156792900409459 >1k 6.580146442841194E-4 0.6125398220897966 >5k 8.225183053551492E-5 0.4451462749479219 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8640 0.4276952473355676 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2684 0.13286273655655823 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 4.950176473791291E-5 0.0 3 0.0 0.0 0.0 4.950176473791291E-5 0.0 4 0.0 0.0 0.0 4.950176473791291E-5 0.0 5 0.0 0.0 0.0 4.950176473791291E-5 0.0 6 0.0 0.0 0.0 4.950176473791291E-5 0.0 7 0.0 0.0 0.0 9.900352947582581E-5 0.0 8 0.0 0.0 0.0 9.900352947582581E-5 0.0 9 0.0 0.0 0.0 9.900352947582581E-5 0.0 10-11 0.0 0.0 0.0 2.2275794132060807E-4 0.0 12-13 0.0 0.0 0.0 4.4551588264121614E-4 0.0 14-15 0.0 0.0 0.0 4.4551588264121614E-4 0.0 16-17 0.0 0.0 0.0 6.930247063307807E-4 0.0 18-19 0.0 0.0 0.0 7.920282358066065E-4 0.0 20-21 0.0 0.0 0.0 0.0010395370594961711 0.0 22-23 0.0 0.0 0.0 0.0014355511773994742 0.0 24-25 0.0 0.0 0.0 0.002004821471885473 0.0 26-27 4.950176473791291E-5 0.0 0.0 0.002574091766371471 0.0 28-29 4.950176473791291E-5 0.0 0.0 0.003564127061129729 0.0 30-31 4.950176473791291E-5 0.0 0.0 0.006336225886452852 4.950176473791291E-5 32-33 4.950176473791291E-5 0.0 0.0 0.01066763030102023 4.950176473791291E-5 34-35 4.950176473791291E-5 0.0 0.0 0.01576631206902526 4.950176473791291E-5 36-37 9.900352947582581E-5 0.0 0.0 0.025889422957928447 4.950176473791291E-5 38-39 9.900352947582581E-5 0.0 0.0 0.043041784439615274 4.950176473791291E-5 40-41 9.900352947582581E-5 0.0 0.0 0.0638325256295387 4.950176473791291E-5 42-43 9.900352947582581E-5 0.0 0.0 0.08350947711285907 4.950176473791291E-5 44-45 9.900352947582581E-5 0.0 0.0 0.10509224653858909 4.950176473791291E-5 46-47 9.900352947582581E-5 0.0 0.0 0.13216971185022747 4.950176473791291E-5 48-49 9.900352947582581E-5 0.0 0.0 0.16219253216377164 4.950176473791291E-5 50-51 9.900352947582581E-5 0.0 0.0 0.1973882868924277 4.950176473791291E-5 52-53 9.900352947582581E-5 0.0 0.0 0.23075247632578103 4.950176473791291E-5 54-55 9.900352947582581E-5 0.0 0.0 0.26644324870181624 4.950176473791291E-5 56-57 9.900352947582581E-5 0.0 0.0 0.3083464925524595 4.950176473791291E-5 58-59 9.900352947582581E-5 0.0 0.0 0.36007583670357846 4.950176473791291E-5 60-61 9.900352947582581E-5 0.0 0.0 0.4167306064461198 4.950176473791291E-5 62-63 9.900352947582581E-5 0.0 0.0 0.47504368530738117 4.950176473791291E-5 64-65 9.900352947582581E-5 0.0 0.0 0.5323914797562533 9.900352947582581E-5 66-67 9.900352947582581E-5 0.0 0.0 0.5999613886235045 9.900352947582581E-5 68-69 9.900352947582581E-5 0.0 0.0 0.6825798339710811 9.900352947582581E-5 70-71 9.900352947582581E-5 0.0 0.0 0.7738115863830546 9.900352947582581E-5 72-73 9.900352947582581E-5 0.0 0.0 0.8727408632117735 9.900352947582581E-5 74-75 9.900352947582581E-5 0.0 0.0 0.9723136629820853 9.900352947582581E-5 76-77 9.900352947582581E-5 0.0 0.0 1.0780246815798984 9.900352947582581E-5 78-79 9.900352947582581E-5 0.0 0.0 1.2039324201907797 9.900352947582581E-5 80-81 9.900352947582581E-5 0.0 0.0 1.3524872161692565 9.900352947582581E-5 82-83 9.900352947582581E-5 0.0 0.0 1.5074029889165548 9.900352947582581E-5 84-85 9.900352947582581E-5 0.0 0.0 1.6615762351927845 9.900352947582581E-5 86-87 9.900352947582581E-5 0.0 0.0 1.8278526629474339 9.900352947582581E-5 88-89 9.900352947582581E-5 0.0 0.0 2.0195482468950017 9.900352947582581E-5 90-91 9.900352947582581E-5 0.0 0.0 2.241167647626638 9.900352947582581E-5 92-93 9.900352947582581E-5 0.0 0.0 2.485458856608238 9.900352947582581E-5 94-95 9.900352947582581E-5 0.0 0.0 2.7231415799973266 9.900352947582581E-5 96-97 9.900352947582581E-5 0.0 0.0 2.965155707800983 9.900352947582581E-5 98-99 9.900352947582581E-5 0.0 0.0 3.234692816798919 9.900352947582581E-5 100-101 9.900352947582581E-5 0.0 0.0 3.554573220535312 9.900352947582581E-5 102-103 9.900352947582581E-5 0.0 0.0 3.895095860167415 9.900352947582581E-5 104-105 9.900352947582581E-5 0.0 0.0 4.227871473618034 1.4850529421373871E-4 106-107 9.900352947582581E-5 4.950176473791291E-5 0.0 4.555771163241969 1.4850529421373871E-4 108-109 9.900352947582581E-5 4.950176473791291E-5 0.0 4.912258122002049 1.4850529421373871E-4 110-111 9.900352947582581E-5 4.950176473791291E-5 0.0 5.333542890804058 1.4850529421373871E-4 112-113 9.900352947582581E-5 4.950176473791291E-5 2.4750882368956454E-5 5.790345175805517 1.4850529421373871E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 9360 0.0 32.52153 1 GTACATG 11425 0.0 31.388184 1 TACATGG 11540 0.0 30.094604 2 ACATGGG 11995 0.0 28.95304 3 TCAACGC 11105 0.0 27.571455 4 ATCAACG 11165 0.0 27.477322 3 CAACGCA 11230 0.0 27.159145 5 AACGCAG 11185 0.0 26.445232 6 CGCATAG 205 7.778726E-8 26.12079 8 TATCAAC 11675 0.0 26.072933 2 GGTATCA 6705 0.0 25.587013 1 CATGGGA 8720 0.0 25.13891 4 GAGTACT 3810 0.0 23.500488 12-13 ACGCAGA 12680 0.0 22.902014 7 CGCAGAG 12435 0.0 22.24871 8 CGCAGAT 595 0.0 21.999025 8 CATGGGG 3555 0.0 21.615496 4 TATAGCG 140 0.0031350958 21.274918 2 GTACTTT 4360 0.0 21.219025 14-15 TACCTGG 1980 0.0 20.759161 2 >>END_MODULE