FastQCFastQC Report
Thu 26 May 2016
SRR1364596_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364596_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2020130
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102100.5054130179740908No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89400.4425457767569414No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59540.29473350724953346No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25020.12385341537425809No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23420.11593313301619204No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82300.041.1778451
GTATCAA122650.038.4599531
GTACATG117100.031.5345021
ATCAACG153800.030.3685053
TCAACGC154150.030.2632034
TACATGG119400.029.9175422
TATCAAC157100.029.82482
CAACGCA155400.029.5917915
AACGCAG158100.029.2291666
ACATGGG122950.028.2130553
GAGTACT97350.026.1496112-13
CGCAGAG179400.025.4606978
ACGCAGA180300.025.3996647
AGTACTT99000.024.9619212-13
GTACTTT102450.024.81881314-15
CATGGGA88700.024.68694
GCAGAGT189650.023.7083999
CATGGGG37100.021.9729824
AGAGTAC176950.021.3140410-11
ACTTTTT125850.020.89330316-17
Produced by FastQC (version 0.11.5)