##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364596_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2020130 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.494384024790484 31.0 30.0 33.0 26.0 34.0 2 29.620195729977773 31.0 30.0 34.0 26.0 34.0 3 29.579539930598525 31.0 30.0 34.0 25.0 34.0 4 33.25101701375654 35.0 33.0 37.0 28.0 37.0 5 32.65388118586428 35.0 33.0 37.0 25.0 37.0 6 32.619961091612915 35.0 33.0 37.0 25.0 37.0 7 32.426858667511496 35.0 33.0 37.0 23.0 37.0 8 32.34874636780801 35.0 33.0 37.0 23.0 37.0 9 33.6207486646899 38.0 33.0 39.0 22.0 39.0 10-11 33.406203313648135 37.0 33.0 39.0 19.5 39.0 12-13 33.267816427655646 37.0 33.0 39.0 18.0 39.0 14-15 34.22004920475415 38.0 33.0 41.0 17.5 41.0 16-17 34.14659749620074 38.0 33.0 41.0 17.5 41.0 18-19 34.20763193457847 38.5 33.0 41.0 17.5 41.0 20-21 34.24301802359254 39.0 33.0 41.0 18.0 41.0 22-23 34.231844237747076 39.0 33.0 41.0 18.0 41.0 24-25 34.223611599253516 39.0 33.0 41.0 18.0 41.0 26-27 34.12076401023697 39.0 33.0 41.0 18.0 41.0 28-29 33.88492225747848 38.0 33.0 41.0 17.0 41.0 30-31 33.79213713968903 38.0 33.0 41.0 16.5 41.0 32-33 33.59000584120824 38.0 33.0 40.0 16.0 41.0 34-35 33.439415532663745 38.0 32.0 40.0 15.5 41.0 36-37 33.263635013588235 38.0 32.0 40.0 11.0 41.0 38-39 33.12767717919144 38.0 32.0 40.0 9.0 41.0 40-41 32.95157366110102 38.0 32.0 40.0 8.5 41.0 42-43 32.76890398142694 38.0 31.5 40.0 7.5 41.0 44-45 32.53990461009935 38.0 31.0 40.0 2.0 41.0 46-47 32.25003514625296 38.0 31.0 40.0 2.0 41.0 48-49 31.964502284506445 38.0 30.0 40.0 2.0 41.0 50-51 31.761897749154755 38.0 30.0 40.0 2.0 41.0 52-53 31.605086553835648 37.0 30.0 40.0 2.0 41.0 54-55 31.394394420161078 37.0 30.0 40.0 2.0 41.0 56-57 31.17826946780653 37.0 29.0 40.0 2.0 41.0 58-59 31.002194660739654 36.5 29.0 40.0 2.0 41.0 60-61 30.78602193918213 36.0 29.0 40.0 2.0 41.0 62-63 30.525477320766484 36.0 28.5 39.5 2.0 41.0 64-65 30.269913322409945 35.0 28.0 39.0 2.0 41.0 66-67 30.000947711285907 35.0 28.0 39.0 2.0 41.0 68-69 29.696582398162494 35.0 28.0 39.0 2.0 40.0 70-71 29.440104844737714 35.0 28.0 38.0 2.0 40.0 72-73 29.283460470365767 35.0 28.0 37.0 2.0 40.0 74-75 28.996400974194728 35.0 28.0 37.0 2.0 39.0 76-77 28.702824818204768 34.0 28.0 36.5 2.0 39.0 78-79 28.391976011444807 34.0 27.0 36.0 2.0 39.0 80-81 28.112634830431702 34.0 26.5 36.0 2.0 37.5 82-83 27.85567463480073 34.0 26.0 35.0 2.0 37.0 84-85 27.82428111062159 34.0 27.0 35.0 2.0 37.0 86-87 27.664439912282873 34.0 27.0 35.0 2.0 36.0 88-89 27.50929791647072 34.0 26.5 35.0 2.0 36.0 90-91 27.351446689074464 34.0 26.0 35.0 2.0 36.0 92-93 27.214984184186164 34.0 26.0 35.0 2.0 35.5 94-95 27.121787459222922 34.0 26.0 35.0 2.0 35.0 96-97 27.03116086588487 34.0 26.0 35.0 2.0 35.0 98-99 26.93562444001129 34.0 25.5 35.0 2.0 35.0 100-101 26.834147059842685 34.0 25.0 35.0 2.0 35.0 102-103 25.666897922410932 31.5 23.5 34.0 2.0 35.0 104-105 26.572506967373386 33.5 25.0 35.0 2.0 35.0 106-107 26.739450183899056 34.0 25.0 35.0 2.0 35.0 108-109 26.74579210248845 34.0 25.0 35.0 2.0 35.0 110-111 26.68490616940494 34.0 25.0 35.0 2.0 35.0 112-113 26.634583170390023 34.0 25.0 35.0 2.0 35.0 114-115 26.55278150416062 34.0 25.0 35.0 2.0 35.0 116-117 26.43384485156896 34.0 25.0 35.0 2.0 35.0 118-119 26.328320207115382 34.0 25.0 35.0 2.0 35.0 120-121 26.187545355991944 34.0 24.0 35.0 2.0 35.0 122-123 25.999069614331752 34.0 24.0 35.0 2.0 35.0 124-125 25.204117309282076 33.0 20.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.9533919610060231 1101 2 0.9212023957569642 1101 3 0.7581245130816718 1101 4 0.8755364247583586 1101 5 1.137758440851293 1101 6 1.0562240063093142 1101 7 0.9911097091657552 1101 8 1.0692758484345077 1101 9 1.0881567790345414 1101 10-11 1.225518628880522 1101 12-13 1.2038202262256519 1101 14-15 1.2572328683694565 1101 16-17 1.384622114380086 1101 18-19 1.3284428841373312 1101 20-21 1.4564949847091242 1101 22-23 1.3763956401293953 1101 24-25 1.3268914583873155 1101 26-27 1.339585360947538 1101 28-29 1.4659782387571312 1101 30-31 1.5027383475548675 1101 32-33 1.5241357834699514 1101 34-35 1.4559793434668364 1101 36-37 1.5454715154475522 1101 38-39 1.5613881251107955 1101 40-41 1.5851662457842721 1101 42-43 1.5309622660235505 1101 44-45 1.4636884510659556 1101 46-47 1.5205283677990664 1101 48-49 1.5383050711926778 1101 50-51 1.5736531530375828 1101 52-53 1.5254385082726003 1101 54-55 1.4540037376649906 1101 56-57 1.4006135952485472 1101 58-59 1.527712180356982 1101 60-61 1.3925816975162775 1101 62-63 1.361786131178846 1101 64-65 1.4151914284907718 1101 66-67 1.3659073642678692 1101 68-69 1.3940575464834986 1101 70-71 1.466855577342816 1101 72-73 1.406336337275576 1101 74-75 1.376124319908815 1101 76-77 1.4842984751349846 1101 78-79 1.4597977406320588 1101 80-81 1.4453365194764878 1101 82-83 1.4424154855675368 1101 84-85 1.4163120834797418 1101 86-87 1.317345098251387 1101 88-89 1.2920282468414612 1101 90-91 1.2730765293848378 1101 92-93 1.3046451086882769 1101 94-95 1.3591250950635114 1101 96-97 1.331032059406315 1101 98-99 1.3530697003406083 1101 100-101 1.3340685100564542 1101 102-103 1.33731971841069 1101 104-105 1.15239960406468 1101 106-107 1.2424723582907191 1101 108-109 1.2998475573230088 1101 110-111 1.4331443047089216 1101 112-113 1.343437599837845 1101 114-115 1.3072652295148721 1101 116-117 1.3251300950368972 1101 118-119 1.3693384677033293 1101 120-121 1.4506269632719828 1101 122-123 1.4726502831355468 1101 124-125 1.282742128044756 1102 1 0.43406556280321595 1102 2 -0.44806481897276385 1102 3 0.11293650170031455 1102 4 0.3599191093034335 1102 5 0.7510919324388254 1102 6 0.6636489695421872 1102 7 0.64390794909135 1102 8 0.5336796599149167 1102 9 0.8942044991490476 1102 10-11 1.0152132054732164 1102 12-13 1.0541365907526838 1102 14-15 1.0674480868843403 1102 16-17 1.2153171883944864 1102 18-19 1.0210757556201742 1102 20-21 0.9771020010528275 1102 22-23 1.0026052866578539 1102 24-25 0.971618680303223 1102 26-27 1.0860453044723641 1102 28-29 1.136861318757866 1102 30-31 1.0351464513207134 1102 32-33 1.0569885455631791 1102 34-35 0.8040351906536998 1102 36-37 0.8162963830805054 1102 38-39 0.8236131539472211 1102 40-41 0.7511242573490549 1102 42-43 0.6964463782013155 1102 44-45 0.5483901976527861 1102 46-47 0.6356336949590471 1102 48-49 0.5325199271615766 1102 50-51 0.5163309651157562 1102 52-53 0.6059977893957154 1102 54-55 0.6050602462824699 1102 56-57 0.4750080301677606 1102 58-59 0.5759388881265721 1102 60-61 0.5209340878919768 1102 62-63 0.5588964455040504 1102 64-65 0.3864232971455408 1102 66-67 0.45732236180234054 1102 68-69 0.2580306188124979 1102 70-71 0.11224336666475665 1102 72-73 0.279743967357831 1102 74-75 0.37261717311330145 1102 76-77 0.48905446334792657 1102 78-79 0.4066460723271028 1102 80-81 0.29021820108559027 1102 82-83 0.3664988430885856 1102 84-85 0.35178413237165884 1102 86-87 0.26678482444285123 1102 88-89 0.3803531333570156 1102 90-91 0.35238135825990113 1102 92-93 0.43906491103597745 1102 94-95 0.2560433463402454 1102 96-97 0.2947573919829054 1102 98-99 0.2621233150642901 1102 100-101 0.08050207188727043 1102 102-103 0.041920599526900304 1102 104-105 0.21817957965756563 1102 106-107 0.42351077197941933 1102 108-109 0.36450040336289646 1102 110-111 0.3003982562982408 1102 112-113 0.3452461755608631 1102 114-115 0.4468134389585465 1102 116-117 0.47461799884136724 1102 118-119 0.34782907873357516 1102 120-121 0.36658227732204196 1102 122-123 0.25035246456175386 1102 124-125 0.15209960161720204 1103 1 0.36806076254812226 1103 2 -1.092949789520567 1103 3 0.05267929593139087 1103 4 0.32997654862157333 1103 5 0.5710106211159953 1103 6 0.6777500420587899 1103 7 0.47975322919958785 1103 8 0.6308781465849407 1103 9 0.7582303791599045 1103 10-11 0.857163944686647 1103 12-13 0.8306752362534198 1103 14-15 0.8881223632911315 1103 16-17 0.9844831979346722 1103 18-19 1.0871901128642918 1103 20-21 1.114760950253725 1103 22-23 1.0116098585345625 1103 24-25 0.8808297622565888 1103 26-27 0.9771315453210718 1103 28-29 1.134539723211681 1103 30-31 0.9547875309902878 1103 32-33 0.9858858442347085 1103 34-35 0.9664013415299451 1103 36-37 1.1466744785771397 1103 38-39 1.1057311264642564 1103 40-41 1.1832523560774675 1103 42-43 1.2056340836879968 1103 44-45 1.284285354738337 1103 46-47 1.1573127285306697 1103 48-49 1.1270570515760845 1103 50-51 1.3061490595782246 1103 52-53 1.3124250250262008 1103 54-55 1.242926459639591 1103 56-57 1.21071746822766 1103 58-59 1.1778850597723185 1103 60-61 1.135564631992704 1103 62-63 1.2417626804063033 1103 64-65 1.2622583864070194 1103 66-67 1.2392635565229213 1103 68-69 1.1469039592988146 1103 70-71 1.1862836388228466 1103 72-73 1.1029364386273954 1103 74-75 1.0790664925026228 1103 76-77 0.9921741982566346 1103 78-79 1.0495970442279408 1103 80-81 1.0949658860613063 1103 82-83 1.049332652569305 1103 84-85 1.1419937018568582 1103 86-87 1.0740789284024146 1103 88-89 1.0621752912311173 1103 90-91 1.1529566132465199 1103 92-93 1.1704249258590238 1103 94-95 1.0803551948693588 1103 96-97 1.1143805891941057 1103 98-99 1.0371767469881412 1103 100-101 1.0827511680690414 1103 102-103 1.161138101929179 1103 104-105 1.1465818789539028 1103 106-107 1.1185291068855463 1103 108-109 1.1258709537548022 1103 110-111 1.0909284173265483 1103 112-113 1.1193111168934742 1103 114-115 1.0706902913011511 1103 116-117 1.1804854919792405 1103 118-119 1.2423023218359042 1103 120-121 1.186194701967949 1103 122-123 1.2427003882575214 1103 124-125 1.1443498181457663 1104 1 0.14844405207365696 1104 2 -0.923171940323968 1104 3 -0.11341888964787117 1104 4 0.19537820936429995 1104 5 0.38376353621558934 1104 6 0.5062393298728196 1104 7 0.5025259502695434 1104 8 0.6196867530633909 1104 9 0.5449122350800764 1104 10-11 0.7054703620505975 1104 12-13 0.5770628466178138 1104 14-15 0.7007222380531175 1104 16-17 0.7288237343665642 1104 18-19 0.7326158965902465 1104 20-21 0.7926416476585416 1104 22-23 0.7204783547984661 1104 24-25 0.7170467900346935 1104 26-27 0.612421276186879 1104 28-29 0.7821944756880583 1104 30-31 0.6694803280192687 1104 32-33 0.7288528436365738 1104 34-35 0.8032123571183831 1104 36-37 0.8285866729587781 1104 38-39 0.8410271383605874 1104 40-41 0.8355804184465043 1104 42-43 0.8254060025715262 1104 44-45 0.7705593900421874 1104 46-47 0.8040352102837929 1104 48-49 0.8197923861669061 1104 50-51 0.7966301099960873 1104 52-53 0.564090773450836 1104 54-55 0.7533118407164068 1104 56-57 0.7956585221909656 1104 58-59 0.8250751116402419 1104 60-61 0.8549178261358499 1104 62-63 0.8225376894293035 1104 64-65 0.8362385638572896 1104 66-67 0.7444026829431678 1104 68-69 0.765490293816935 1104 70-71 0.810182226643132 1104 72-73 0.748302122137428 1104 74-75 0.6680310725918233 1104 76-77 0.6339160141057505 1104 78-79 0.6440750618964479 1104 80-81 0.6317478558689977 1104 82-83 0.654509146338647 1104 84-85 0.6574094869474116 1104 86-87 0.7026299424859523 1104 88-89 0.7357032663671639 1104 90-91 0.7289988498290292 1104 92-93 0.6952174605513548 1104 94-95 0.7247323334486602 1104 96-97 0.7188581987550435 1104 98-99 0.7759794550369143 1104 100-101 0.7759403629110722 1104 102-103 0.7621401016562608 1104 104-105 0.7301690041804356 1104 106-107 0.7406859043634455 1104 108-109 0.6098264279439114 1104 110-111 0.6477314110506178 1104 112-113 0.7019607106351415 1104 114-115 0.7230121364064708 1104 116-117 0.7586175262551755 1104 118-119 0.5690648045072706 1104 120-121 0.6583788235227885 1104 122-123 0.7316232967381211 1104 124-125 0.6427521407879553 1105 1 0.42347751017454627 1105 2 0.17766588661382343 1105 3 0.38221623932266624 1105 4 0.3516696123215368 1105 5 0.4029144957255184 1105 6 0.3228938681090625 1105 7 0.4577516880579182 1105 8 0.5029613117710809 1105 9 0.49774795863747556 1105 10-11 0.5233571005561899 1105 12-13 0.5506472615427569 1105 14-15 0.5555583552599188 1105 16-17 0.7763456211474633 1105 18-19 0.7981253683068701 1105 20-21 0.8203970780688365 1105 22-23 0.7233885485973559 1105 24-25 0.680480306721357 1105 26-27 0.6391154164763151 1105 28-29 0.899746664659915 1105 30-31 0.7988577891149191 1105 32-33 0.711822943068718 1105 34-35 0.7044662123015613 1105 36-37 0.6565436808204339 1105 38-39 0.7069657005162782 1105 40-41 0.6126880374929229 1105 42-43 0.5838402977439472 1105 44-45 0.6784732328619256 1105 46-47 0.6093845510469507 1105 48-49 0.5696430889653818 1105 50-51 0.5903485377821909 1105 52-53 0.44032255405130627 1105 54-55 0.4664362188195952 1105 56-57 0.43670293532811044 1105 58-59 0.38997160450943724 1105 60-61 0.4627591396826354 1105 62-63 0.48410658583865995 1105 64-65 0.4843945283381963 1105 66-67 0.4007583476965628 1105 68-69 0.3654099901781116 1105 70-71 0.45947570360393897 1105 72-73 0.37523275632844033 1105 74-75 0.4485836484983814 1105 76-77 0.43023271680098674 1105 78-79 0.4335395686113621 1105 80-81 0.28772214653417905 1105 82-83 0.4333222996843986 1105 84-85 0.2284180372810276 1105 86-87 0.3376409336137307 1105 88-89 0.37467913162995004 1105 90-91 0.3892582219792118 1105 92-93 0.1710294253948561 1105 94-95 0.28356896516949703 1105 96-97 0.24992507886529935 1105 98-99 0.3500527946904626 1105 100-101 0.43488024366635614 1105 102-103 -0.046514131480712706 1105 104-105 -0.16145339791186686 1105 106-107 0.2297742513758827 1105 108-109 0.3077238029570779 1105 110-111 0.2433094230875632 1105 112-113 0.004815749452415474 1105 114-115 0.10276011265824891 1105 116-117 -0.07571996977370787 1105 118-119 -0.12458447234031311 1105 120-121 0.013180790175059798 1105 122-123 0.09893412364947096 1105 124-125 -0.10032988013589517 1106 1 0.5538745729236716 1106 2 0.5003902184706881 1106 3 0.6582746133380404 1106 4 0.505653586840026 1106 5 0.7165628490643101 1106 6 0.7449958665436114 1106 7 0.7496195027197388 1106 8 0.6973754677638979 1106 9 0.851487162249768 1106 10-11 0.9460648051842142 1106 12-13 0.7744921781351408 1106 14-15 0.8658450675025335 1106 16-17 1.0376278180228908 1106 18-19 0.9970390560226292 1106 20-21 1.1380970459454787 1106 22-23 1.0621021666371036 1106 24-25 1.01110313782943 1106 26-27 1.089764783284295 1106 28-29 1.237496582089328 1106 30-31 1.1503817255378266 1106 32-33 1.0602272946520443 1106 34-35 0.9981771609358674 1106 36-37 1.0798587882979334 1106 38-39 1.1477761591563294 1106 40-41 1.1400918597195755 1106 42-43 1.1352274836929155 1106 44-45 1.0555618164030065 1106 46-47 0.8792280741192045 1106 48-49 1.0114613612718806 1106 50-51 1.1902346356091158 1106 52-53 1.2170709015083645 1106 54-55 1.146280058600972 1106 56-57 0.9420688927171632 1106 58-59 1.005356089382051 1106 60-61 1.3354482942050154 1106 62-63 1.1413441810210614 1106 64-65 1.121940733936711 1106 66-67 1.060198202372323 1106 68-69 1.135462201938882 1106 70-71 1.1434921860514642 1106 72-73 1.2263941739184645 1106 74-75 1.2021573715950709 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-3.249309680742286 2216 74-75 -2.903459944329711 2216 76-77 -3.266336095742023 2216 78-79 -2.9378456136513726 2216 80-81 -2.626754733074229 2216 82-83 -2.6029788693046783 2216 84-85 -2.575956312731517 2216 86-87 -2.5838598445558887 2216 88-89 -2.4910416293223108 2216 90-91 -2.50056764391098 2216 92-93 -2.5711475293276926 2216 94-95 -2.41837996850818 2216 96-97 -2.415946374290826 2216 98-99 -2.373478344599885 2216 100-101 -2.6382663894692264 2216 102-103 -2.387960054541562 2216 104-105 -2.3885252291398267 2216 106-107 -2.4695877423196926 2216 108-109 -2.5657268165530738 2216 110-111 -2.4320044467269604 2216 112-113 -2.490971787247421 2216 114-115 -2.4902600003437563 2216 116-117 -2.5910939631756023 2216 118-119 -2.7433931023180342 2216 120-121 -2.7249158166181324 2216 122-123 -2.6806717207311266 2216 124-125 -2.702524346700418 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 85736.0 3 26995.0 4 12252.0 5 9588.0 6 10444.0 7 12266.0 8 13560.0 9 14381.0 10 15535.0 11 16863.0 12 18797.0 13 21466.0 14 24303.0 15 20162.0 16 12977.0 17 11251.0 18 10193.0 19 9444.0 20 9145.0 21 9625.0 22 11278.0 23 14665.0 24 20300.0 25 26676.0 26 31903.0 27 35014.0 28 40305.0 29 46938.0 30 54075.0 31 64041.0 32 77493.0 33 96079.0 34 123389.0 35 169077.0 36 261259.0 37 411979.0 38 169936.0 39 740.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.47095832452082 19.746655247958177 13.681894528183292 24.10049189933771 2 15.378590737980344 21.14997738566385 39.238325379923324 24.233106496432484 3 19.16104409122185 25.888135912045264 29.34756674075431 25.603253255978576 4 12.393951320456745 17.6099349239707 36.69971212087458 33.29640163469797 5 12.825921612435529 39.23092006750172 33.62890095785896 14.314257362203788 6 30.849275439800174 37.80301387737968 17.162181294001055 14.18552938881909 7 27.587333488438865 33.90851083841139 20.381015083187716 18.123140589962034 8 25.977387593867725 36.34206709469193 19.05248672115161 18.62805859028874 9 26.4724795098096 16.580409115727683 19.696125909788215 37.2509854646745 10-11 24.58851479479419 27.84676486853351 26.622531218787714 20.94218911788459 12-13 25.749550045954596 25.147919839736904 27.638354525915148 21.464175588393353 14-15 22.641065283675147 26.043815523901603 26.369513591545218 24.945605600878032 16-17 21.495716844173955 29.537604404399648 26.872295787371627 22.094382964054773 18-19 21.310665121549288 27.471837166673186 30.21777838720041 20.999719324577118 20-21 22.27103615746207 26.476633917763863 30.258476223009385 20.993853701764678 22-23 21.785689885130537 26.445670933303234 30.164843271160645 21.603795910405584 24-25 21.567374376896538 26.512130407449025 30.40447891967349 21.516016295980954 26-27 21.56608733101335 26.804562082638245 30.21441689396227 21.414933692386136 28-29 21.269929004565707 26.57931967310749 30.597078313394732 21.553673008932066 30-31 21.415354457386407 26.513788716567742 30.563800349482463 21.50705647656339 32-33 21.283094412327593 26.580254785268114 30.347975846190252 21.78867495621404 34-35 21.680634414616883 26.51824387539416 30.264240420170978 21.536881289817984 36-37 21.764852058586502 26.268857753286433 30.233886632701758 21.73240355542531 38-39 21.658705132837987 26.525273125986942 30.313098661967295 21.502923079207772 40-41 21.71545890611 26.37010984441595 30.401706820848162 21.51272442862588 42-43 21.581179974268103 26.457196094573384 30.028895167027137 21.93272876413138 44-45 21.67350616049462 26.790553083217418 29.63002381034884 21.905916945939122 46-47 21.7440924535475 26.920769376989973 29.08328086141076 22.25185730805177 48-49 21.996006679789627 27.005131121933886 28.835935030704807 22.162927167571684 50-51 22.052980099667494 27.205330186320893 28.546267369662253 22.19542234434936 52-53 22.141036537765657 27.29494007560903 28.39336002799821 22.170663358627095 54-55 21.81183884205472 27.210427051724395 28.59281333379535 22.384920772425538 56-57 22.021032663794195 27.533369701241924 28.337539404067925 22.108058230895956 58-59 22.092172803773224 27.02534664238109 28.537551676761318 22.344928877084367 60-61 22.090632781058645 27.077935578403373 28.750204194779545 22.08122744575844 62-63 22.02808730131229 27.00751931806369 28.786984005979814 22.177409374644206 64-65 21.957710328372396 26.794563675830336 28.753288797914973 22.4944371978823 66-67 21.95014677273245 27.311014637671832 28.52286734022068 22.21597124937504 68-69 21.989546160399463 27.150083732690995 28.628684745799625 22.231685361109914 70-71 21.749367614955474 27.133773569027735 28.66466514531243 22.45219367070436 72-73 22.0434553495207 27.074509912634365 28.49926005167634 22.382774686168595 74-75 21.966397274911518 27.24336023290576 28.364597908610428 22.425644583572293 76-77 22.11997546671894 27.115215831985633 28.535408662782725 22.229400038512697 78-79 22.127249020098986 27.12600650858224 28.44819221999681 22.29855225132196 80-81 21.838135361020225 27.52965630687369 28.41933932527648 22.212869006829607 82-83 22.051347292198606 27.402894670926464 28.160273171079425 22.385484865795497 84-85 21.879845762286905 27.12456163060914 28.314733443936856 22.680859163167096 86-87 21.937192964392285 27.078164508713325 28.448228920600915 22.536413606293475 88-89 22.125704781375454 26.91809438006465 28.42455683545118 22.53164400310871 90-91 21.830624761772757 27.246192076747537 28.55717206318405 22.366011098295655 92-93 21.819382558761525 27.218212390261122 28.47957056692305 22.482834484054308 94-95 21.729398366280254 27.123802253623346 28.346480665883988 22.800318714212413 96-97 21.89530875809875 27.053162457688472 28.334729794120012 22.71679899009276 98-99 21.817378530598 26.949828165085275 28.22607212734966 23.00672117696707 100-101 21.71382845726282 27.105140510182434 28.43728876569492 22.743742266859822 102-103 22.01878346787517 26.931146672672888 28.31947488462981 22.730594974822132 104-105 21.871975732098687 26.740876889803904 28.497705825949687 22.889441552147723 106-107 21.771033670208684 27.11876279371397 28.34250396651543 22.767699569561913 108-109 21.879066382672214 27.137155212209947 27.92178683831747 23.061991566800373 110-111 21.587343232158723 27.06089534949916 28.262268545446894 23.08949287289522 112-113 21.83093571478075 26.966104967179195 28.165896062493196 23.037063255546865 114-115 21.474972513995464 27.098552114999165 28.156361085658816 23.27011428534656 116-117 21.423870408996482 26.99449551094178 28.313280894777108 23.26835318528463 118-119 21.562558475464808 27.18944516003837 27.941940855749138 23.306055508747683 120-121 21.39893185798538 27.096176101095992 28.072133622957008 23.43275841796162 122-123 21.447963980099058 27.082323175695528 27.878444962404192 23.591267881801222 124-125 21.30766492896133 27.05337911991355 27.953707173467414 23.685248777657705 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 668.0 1 505.0 2 618.5 3 1015.5 4 798.5 5 603.0 6 1423.5 7 3324.5 8 5196.0 9 5784.5 10 4527.0 11 2765.5 12 2201.0 13 2208.0 14 2318.0 15 2506.0 16 2710.5 17 2967.0 18 3207.5 19 3468.5 20 3840.5 21 4284.5 22 4953.5 23 6015.5 24 7274.0 25 8724.5 26 10937.0 27 13676.5 28 16492.0 29 19629.5 30 23592.0 31 27781.5 32 31742.0 33 36309.5 34 41753.0 35 45693.0 36 49871.0 37 55706.0 38 59867.5 39 62002.5 40 62815.0 41 65237.0 42 68371.5 43 70684.5 44 72924.0 45 74045.0 46 73419.0 47 73717.5 48 80967.5 49 83571.0 50 79221.5 51 74859.0 52 68768.0 53 65902.0 54 63348.5 55 57470.5 56 50716.0 57 45121.0 58 40144.0 59 35349.5 60 30346.5 61 24353.0 62 19047.0 63 14528.0 64 10834.5 65 8545.0 66 6457.0 67 4867.5 68 3807.0 69 2710.0 70 1992.0 71 1568.5 72 1330.5 73 1247.5 74 978.0 75 756.0 76 555.5 77 325.5 78 210.0 79 123.5 80 78.0 81 61.0 82 41.5 83 30.5 84 22.5 85 12.5 86 8.0 87 5.0 88 2.0 89 1.0 90 0.5 91 0.5 92 0.5 93 1.5 94 1.0 95 1.0 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.060441654744991656 2 0.07420314534213145 3 0.0 4 0.009157826476513886 5 0.030988104725933482 6 3.9601411790330326E-4 7 0.0 8 0.0 9 8.415300005445195E-4 10-11 0.05908035621469905 12-13 0.00997460559468945 14-15 0.007969784122803978 16-17 0.052372867092711856 18-19 1.4850529421373871E-4 20-21 0.010642879418651276 22-23 0.013464480008712311 24-25 0.0 26-27 0.0 28-29 0.05229861444560499 30-31 0.0 32-33 0.003762134120081381 34-35 0.0 36-37 2.9701058842747743E-4 38-39 0.0 40-41 0.0 42-43 0.0034156217669159905 44-45 0.0 46-47 9.900352947582581E-5 48-49 3.217614707964339E-4 50-51 6.435229415928678E-4 52-53 4.950176473791291E-5 54-55 0.0 56-57 0.001683060001089039 58-59 2.4750882368956454E-5 60-61 0.0 62-63 0.0 64-65 7.425264710686936E-4 66-67 0.0 68-69 5.44519412117042E-4 70-71 0.0 72-73 0.0027968497076920795 74-75 0.004232400885091554 76-77 8.415300005445195E-4 78-79 0.0013860494126615615 80-81 0.0014108002950305178 82-83 1.9800705895165163E-4 84-85 7.425264710686936E-5 86-87 2.9701058842747743E-4 88-89 0.0 90-91 0.0 92-93 0.008143040299386674 94-95 0.006855994416200938 96-97 0.007054001475152589 98-99 0.0011137897066030404 100-101 4.702667650101726E-4 102-103 1.2375441184478227E-4 104-105 6.435229415928678E-4 106-107 0.004702667650101726 108-109 0.006806492651463025 110-111 0.0012375441184478227 112-113 0.0034651235316539033 114-115 5.44519412117042E-4 116-117 0.0025493408840025146 118-119 0.002574091766371471 120-121 0.013241722067391704 122-123 0.001212793236078866 124-125 0.0016335582363511258 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2020130.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.99137090027044 #Duplication Level Percentage of deduplicated Percentage of total 1 77.35921095778822 42.540890623319974 2 11.910933622559707 13.099971372133654 3 4.370970221724671 7.21096933970696 4 2.129152889871511 4.683401450812277 5 1.1266893869738444 3.09790969842385 6 0.7165522820768908 2.364251538787531 7 0.4757709370396289 1.8314307243620813 8 0.33617361611998403 1.4789318408751342 9 0.23727466664274136 1.1743253278730115 >10 1.1985457819164629 11.686816813897511 >50 0.08786884569791956 3.3568226203994826 >100 0.04723197374972507 4.654254930682368 >500 0.0027186133791030523 1.0179972172354523 >1k 6.343431217907112E-4 0.5510081863605043 >5k 1.812408919402032E-4 0.742219040214647 >10k+ 9.06204459701016E-5 0.5087992749155059 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10210 0.5054130179740908 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8940 0.4425457767569414 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5954 0.29473350724953346 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2502 0.12385341537425809 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2342 0.11593313301619204 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 4.950176473791291E-5 0.0 3 0.0 0.0 0.0 9.900352947582581E-5 0.0 4 0.0 0.0 0.0 9.900352947582581E-5 0.0 5 0.0 0.0 0.0 9.900352947582581E-5 0.0 6 0.0 0.0 0.0 9.900352947582581E-5 0.0 7 0.0 0.0 0.0 9.900352947582581E-5 0.0 8 0.0 0.0 0.0 9.900352947582581E-5 0.0 9 0.0 0.0 0.0 9.900352947582581E-5 0.0 10-11 0.0 0.0 0.0 9.900352947582581E-5 0.0 12-13 0.0 0.0 0.0 1.4850529421373871E-4 0.0 14-15 0.0 0.0 0.0 1.7325617658269519E-4 0.0 16-17 0.0 0.0 0.0 5.197685297480856E-4 0.0 18-19 0.0 0.0 0.0 7.920282358066065E-4 0.0 20-21 0.0 0.0 0.0 8.662808829134759E-4 0.0 22-23 0.0 0.0 0.0 0.0012870458831857356 0.0 24-25 0.0 0.0 0.0 0.0018810670600406905 0.0 26-27 0.0 0.0 0.0 0.0024750882368956453 0.0 28-29 0.0 0.0 0.0 0.0036878814729745116 0.0 30-31 0.0 0.0 0.0 0.0064352294159286776 0.0 32-33 0.0 0.0 0.0 0.010989391771816666 0.0 34-35 0.0 0.0 0.0 0.016508838540093954 0.0 36-37 0.0 0.0 0.0 0.02680520560557984 0.0 38-39 0.0 0.0 0.0 0.044477335617014745 0.0 40-41 0.0 0.0 0.0 0.06640661739591017 0.0 42-43 0.0 0.0 0.0 0.08680134446793028 0.0 44-45 2.4750882368956454E-5 0.0 0.0 0.10944840183552543 0.0 46-47 4.950176473791291E-5 0.0 0.0 0.13650111626479483 0.0 48-49 4.950176473791291E-5 0.0 0.0 0.16674669451965962 0.0 50-51 4.950176473791291E-5 0.0 0.0 0.20283348101359816 0.0 52-53 4.950176473791291E-5 0.0 0.0 0.23691544603565118 0.0 54-55 4.950176473791291E-5 0.0 0.0 0.27374475900065837 0.0 56-57 4.950176473791291E-5 0.0 0.0 0.3160440169692049 0.0 58-59 4.950176473791291E-5 0.0 0.0 0.3686396420032374 0.0 60-61 4.950176473791291E-5 0.0 0.0 0.4254181661576235 0.0 62-63 4.950176473791291E-5 0.0 0.0 0.48410250825441925 0.0 64-65 4.950176473791291E-5 0.0 0.0 0.5414750535856603 0.0 66-67 4.950176473791291E-5 0.0 0.0 0.6095152292179216 0.0 68-69 4.950176473791291E-5 0.0 0.0 0.6933217169192082 0.0 70-71 4.950176473791291E-5 0.0 0.0 0.7863850346264845 0.0 72-73 4.950176473791291E-5 0.0 0.0 0.8846955393959794 0.0 74-75 4.950176473791291E-5 0.0 0.0 0.9831793003420572 0.0 76-77 4.950176473791291E-5 0.0 0.0 1.0886923118809184 0.0 78-79 4.950176473791291E-5 0.0 0.0 1.2149960646097033 0.0 80-81 4.950176473791291E-5 0.0 0.0 1.3638973729413453 0.0 82-83 4.950176473791291E-5 0.0 0.0 1.5189121492181195 0.0 84-85 4.950176473791291E-5 0.0 0.0 1.6742734378480593 0.0 86-87 4.950176473791291E-5 0.0 0.0 1.8412428903090396 0.0 88-89 4.950176473791291E-5 0.0 0.0 2.0331859830802967 0.0 90-91 4.950176473791291E-5 0.0 0.0 2.254830134694302 0.0 92-93 4.950176473791291E-5 0.0 0.0 2.4994431051466988 0.0 94-95 4.950176473791291E-5 0.0 0.0 2.7367298144178838 0.0 96-97 4.950176473791291E-5 0.0 0.0 2.9801299916342017 0.0 98-99 4.950176473791291E-5 0.0 0.0 3.2492710865142342 0.0 100-101 4.950176473791291E-5 0.0 0.0 3.5687802270150932 0.0 102-103 4.950176473791291E-5 0.0 0.0 3.9127927410612187 0.0 104-105 4.950176473791291E-5 0.0 0.0 4.248018691866365 0.0 106-107 9.900352947582581E-5 0.0 0.0 4.578220213550614 0.0 108-109 9.900352947582581E-5 0.0 0.0 4.938667313489725 0.0 110-111 9.900352947582581E-5 0.0 0.0 5.362130159940202 0.0 112-113 9.900352947582581E-5 0.0 0.0 5.8211352734725 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8230 0.0 41.177845 1 GTATCAA 12265 0.0 38.459953 1 GTACATG 11710 0.0 31.534502 1 ATCAACG 15380 0.0 30.368505 3 TCAACGC 15415 0.0 30.263203 4 TACATGG 11940 0.0 29.917542 2 TATCAAC 15710 0.0 29.8248 2 CAACGCA 15540 0.0 29.591791 5 AACGCAG 15810 0.0 29.229166 6 ACATGGG 12295 0.0 28.213055 3 GAGTACT 9735 0.0 26.14961 12-13 CGCAGAG 17940 0.0 25.460697 8 ACGCAGA 18030 0.0 25.399664 7 AGTACTT 9900 0.0 24.96192 12-13 GTACTTT 10245 0.0 24.818813 14-15 CATGGGA 8870 0.0 24.6869 4 GCAGAGT 18965 0.0 23.708399 9 CATGGGG 3710 0.0 21.972982 4 AGAGTAC 17695 0.0 21.31404 10-11 ACTTTTT 12585 0.0 20.893303 16-17 >>END_MODULE