FastQCFastQC Report
Thu 26 May 2016
SRR1364595_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364595_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3350626
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA111400.038.0739021
GTACATG160300.036.493081
TACATGG166700.034.127182
CGCATAG5100.032.665868
TCAACGC127550.032.5053524
ATCAACG128650.032.413123
ACATGGG169650.032.1643073
CAACGCA130600.031.793275
TATCAAC137350.031.0539572
AACGCAG130850.030.293516
GGTATCA77450.027.6894071
CATGGGG70850.025.9803334
CATGGGA102450.025.9327744
ACGCAGA150400.025.9161877
ACGCATA6900.025.0107427
CGCAGAG147350.024.8734958
GTTATCA23200.024.1363981
AACGCAT7300.023.6441786
AGTACAT124700.021.9747522
GAGTACA146450.021.1110861
Produced by FastQC (version 0.11.5)