##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364595_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3350626 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.177100637313742 31.0 27.0 31.0 10.0 34.0 2 26.819727119648686 31.0 26.0 33.0 10.0 34.0 3 27.275671173088252 31.0 27.0 33.0 10.0 34.0 4 30.582654405475274 35.0 30.0 37.0 10.0 37.0 5 30.906549999910464 35.0 32.0 37.0 10.0 37.0 6 31.004140420327424 35.0 32.0 37.0 10.0 37.0 7 30.76447356404445 35.0 31.0 37.0 10.0 37.0 8 30.585589080965764 35.0 31.0 37.0 10.0 37.0 9 31.515734074766925 37.0 30.0 39.0 8.0 39.0 10-11 31.446213931366856 37.0 30.5 39.0 8.0 39.0 12-13 31.483943149727843 37.0 30.0 39.0 8.0 39.0 14-15 32.20383250771647 38.0 30.5 40.0 8.0 41.0 16-17 32.64655828492944 38.0 31.5 40.0 7.5 41.0 18-19 32.484752699943236 38.0 31.0 40.0 7.0 41.0 20-21 32.37433109514461 38.0 31.0 40.0 7.0 41.0 22-23 32.46956509022493 38.0 31.0 40.0 2.0 41.0 24-25 32.572243365866555 38.0 31.0 40.0 2.0 41.0 26-27 32.374106957923686 38.0 30.5 40.0 2.0 41.0 28-29 32.27318790578238 38.0 30.0 40.0 2.0 41.0 30-31 32.154362050554134 38.0 30.0 40.0 2.0 41.0 32-33 32.043340557853966 38.0 30.0 40.0 2.0 41.0 34-35 32.0977172623862 38.0 30.0 40.0 2.0 41.0 36-37 32.05852652608796 38.0 30.0 40.0 2.0 41.0 38-39 31.77930541934552 38.0 30.0 40.0 2.0 41.0 40-41 31.633995856296703 38.0 30.0 40.0 2.0 41.0 42-43 31.17129082744538 37.0 29.5 40.0 2.0 41.0 44-45 31.098203887870504 37.0 29.0 40.0 2.0 41.0 46-47 31.07058352677977 37.0 29.5 40.0 2.0 41.0 48-49 31.010064537193948 37.0 29.0 40.0 2.0 41.0 50-51 30.786364697223743 37.0 29.0 40.0 2.0 41.0 52-53 30.687252322401847 37.0 28.5 40.0 2.0 41.0 54-55 30.515653492810003 36.5 28.0 40.0 2.0 41.0 56-57 30.296123769110608 36.0 28.0 40.0 2.0 41.0 58-59 30.086382514789772 36.0 28.0 40.0 2.0 41.0 60-61 29.79012160712655 35.0 28.0 39.5 2.0 41.0 62-63 29.615836563078062 35.0 27.5 39.0 2.0 41.0 64-65 29.385487517854873 35.0 27.5 39.0 2.0 40.0 66-67 29.108645966455224 35.0 27.0 39.0 2.0 40.0 68-69 28.856855405527206 34.0 26.0 38.0 2.0 40.0 70-71 28.407675013564628 34.0 26.0 37.5 2.0 40.0 72-73 28.158848227167102 34.0 26.0 37.0 2.0 39.5 74-75 28.109698605574003 34.0 26.0 37.0 2.0 39.0 76-77 27.84427924811662 34.0 26.0 36.0 2.0 39.0 78-79 27.545966037391224 34.0 26.0 36.0 2.0 38.5 80-81 27.133723071449932 33.5 25.0 35.0 2.0 37.0 82-83 27.240372396083597 34.0 26.0 35.0 2.0 37.0 84-85 27.069574760059762 34.0 26.0 35.0 2.0 36.5 86-87 26.94161434311081 34.0 26.0 35.0 2.0 36.0 88-89 26.78005527922245 34.0 25.5 35.0 2.0 36.0 90-91 26.651656735189185 34.0 25.0 35.0 2.0 36.0 92-93 26.529510007980598 33.5 25.0 35.0 2.0 35.0 94-95 26.430013376604848 33.0 25.0 35.0 2.0 35.0 96-97 26.32069962448808 33.0 25.0 35.0 2.0 35.0 98-99 26.234970569678623 33.0 24.5 35.0 2.0 35.0 100-101 26.13371889312624 33.0 24.0 35.0 2.0 35.0 102-103 25.037178574988673 31.5 21.5 34.0 2.0 35.0 104-105 25.85755736390752 33.0 23.5 35.0 2.0 35.0 106-107 26.01284730077305 33.0 24.0 35.0 2.0 35.0 108-109 25.97335408368466 33.0 24.0 35.0 2.0 35.0 110-111 25.910448077463734 33.0 23.0 35.0 2.0 35.0 112-113 25.82128458980501 33.0 23.0 35.0 2.0 35.0 114-115 25.724661451322827 33.0 23.0 35.0 2.0 35.0 116-117 25.617739938745775 33.0 20.5 35.0 2.0 35.0 118-119 25.498136766084905 33.0 19.5 35.0 2.0 35.0 120-121 25.32339091262349 33.0 18.5 35.0 2.0 35.0 122-123 25.127386195892946 33.0 8.5 35.0 2.0 35.0 124-125 24.21042232108269 32.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.541578237878209 1101 2 1.7993278724717285 1101 3 1.350364344165179 1101 4 1.5346255250811005 1101 5 1.2597435100817655 1101 6 1.1932011322075837 1101 7 1.1935117472081345 1101 8 1.4477629559023093 1101 9 1.9363112121103647 1101 10-11 1.940006484839202 1101 12-13 1.8228070237685792 1101 14-15 2.166286793127192 1101 16-17 1.7889796709687928 1101 18-19 1.766192517425246 1101 20-21 2.004446499289827 1101 22-23 1.76530599588515 1101 24-25 1.7966447167561768 1101 26-27 2.0591526320464695 1101 28-29 2.0391497922729016 1101 30-31 2.1752926720365195 1101 32-33 2.1887814772859286 1101 34-35 2.013826118422898 1101 36-37 1.8011405970855279 1101 38-39 1.7671697842064056 1101 40-41 1.8344758336288756 1101 42-43 2.0601642561864963 1101 44-45 1.856210707730007 1101 46-47 1.6397924495384117 1101 48-49 1.4609010635862028 1101 50-51 1.5800489465692813 1101 52-53 1.4195789478258014 1101 54-55 1.3828221194334134 1101 56-57 1.3572520450529595 1101 58-59 1.250124718246024 1101 60-61 1.3648398536175996 1101 62-63 1.2869350870996925 1101 64-65 1.2126551561692196 1101 66-67 1.1458461283652959 1101 68-69 1.018511514012257 1101 70-71 1.0095798934842612 1101 72-73 0.9704376314065826 1101 74-75 0.9320077760939824 1101 76-77 1.1072063332782847 1101 78-79 0.9440852521427097 1101 80-81 1.1269277711662404 1101 82-83 1.1535400429974558 1101 84-85 1.0895526903274764 1101 86-87 1.0543521982789592 1101 88-89 1.0571019584702341 1101 90-91 1.0810659808267644 1101 92-93 1.0958045197177668 1101 94-95 1.0041734963087983 1101 96-97 0.95129234111619 1101 98-99 1.0319211658375025 1101 100-101 1.064987434920912 1101 102-103 0.8481539052833469 1101 104-105 1.068822242520433 1101 106-107 1.148658437249697 1101 108-109 1.1129149473381155 1101 110-111 1.1398186901928717 1101 112-113 1.0810385182264852 1101 114-115 1.1256615189156811 1101 116-117 1.0744424295605235 1101 118-119 1.0547383140213604 1101 120-121 1.059711363555902 1101 122-123 1.0493661803338625 1101 124-125 0.9734608173510608 1102 1 0.861056826026072 1102 2 1.415515529051504 1102 3 1.0183697404417877 1102 4 0.9676798568563214 1102 5 0.7269856713696186 1102 6 0.6241313309775123 1102 7 0.648983063175752 1102 8 0.5606433519378378 1102 9 1.2803292299140914 1102 10-11 1.119028194341574 1102 12-13 1.3342809822659767 1102 14-15 1.5433352680072474 1102 16-17 1.2156303440247598 1102 18-19 0.9934985754753995 1102 20-21 1.4259831973779882 1102 22-23 1.3397786631719768 1102 24-25 1.0445377002465506 1102 26-27 1.3449306482944436 1102 28-29 1.0284087492627663 1102 30-31 1.0353726325898336 1102 32-33 1.2307933132221436 1102 34-35 1.1385461471978928 1102 36-37 1.0736697626298408 1102 38-39 1.2419760302969358 1102 40-41 1.2976196790513264 1102 42-43 1.5591515301925014 1102 44-45 1.1431221895717343 1102 46-47 0.964310037581491 1102 48-49 0.9340434336149421 1102 50-51 0.9823868027289997 1102 52-53 0.9445930187102718 1102 54-55 0.8771508136874786 1102 56-57 0.9331064876594972 1102 58-59 1.0556841399688324 1102 60-61 1.0236094138345564 1102 62-63 0.7725845528400228 1102 64-65 0.6101138869320479 1102 66-67 0.7233211334600647 1102 68-69 0.5501448873537278 1102 70-71 0.9697005880829046 1102 72-73 0.6001594136178987 1102 74-75 0.7067170849604238 1102 76-77 0.4901040290283163 1102 78-79 0.6117040004431118 1102 80-81 0.7677709361718321 1102 82-83 0.7476593133107983 1102 84-85 0.6297726782800375 1102 86-87 0.7395358060440955 1102 88-89 0.610076226832458 1102 90-91 0.7297063123049838 1102 92-93 0.7593117330751298 1102 94-95 0.7585075858751615 1102 96-97 0.5873573921570951 1102 98-99 0.6585890196764517 1102 100-101 0.7013421806421114 1102 102-103 0.5244686771566194 1102 104-105 0.6291431036874933 1102 106-107 0.615191228276732 1102 108-109 0.7395030849155262 1102 110-111 0.6538831459333103 1102 112-113 0.5596012726033273 1102 114-115 0.6537205581290131 1102 116-117 0.7739286373615286 1102 118-119 0.7779858741573165 1102 120-121 0.7488379931587943 1102 122-123 0.6772603949261828 1102 124-125 0.7190777087125824 1103 1 1.0639360946030259 1103 2 1.3813671170949782 1103 3 0.9898755025720476 1103 4 0.8251221396152957 1103 5 0.8643820242759901 1103 6 0.9329854727344795 1103 7 0.9338308478213548 1103 8 1.0867030762267298 1103 9 1.6423976588111806 1103 10-11 1.5622388060831796 1103 12-13 1.5675325595056826 1103 14-15 1.878212815966478 1103 16-17 1.388670363012011 1103 18-19 1.457480484790743 1103 20-21 1.7235914629589786 1103 22-23 1.4351678223677382 1103 24-25 1.4517610193668773 1103 26-27 1.7123376118669356 1103 28-29 1.5745849456351309 1103 30-31 1.7018764348062945 1103 32-33 1.7194047108358177 1103 34-35 1.4971262676672055 1103 36-37 1.369611510781688 1103 38-39 1.5230494022831884 1103 40-41 1.6531876796679406 1103 42-43 1.8954146039907727 1103 44-45 1.5999444046548135 1103 46-47 1.4705807840084049 1103 48-49 1.2291727606308136 1103 50-51 1.21241906573729 1103 52-53 1.0028161871808088 1103 54-55 1.020027042381514 1103 56-57 1.0649451344871075 1103 58-59 1.133222399433393 1103 60-61 1.1725691970227103 1103 62-63 1.0616494519368516 1103 64-65 0.8858152170906308 1103 66-67 0.8706121993574101 1103 68-69 0.9419148096880186 1103 70-71 0.9993641339063082 1103 72-73 0.9746119434140077 1103 74-75 0.9584608419770646 1103 76-77 0.9642180530731324 1103 78-79 0.891725512645948 1103 80-81 0.9872033694168216 1103 82-83 0.9884379616815693 1103 84-85 0.9555378085008499 1103 86-87 0.9872037897217751 1103 88-89 0.9608261230112092 1103 90-91 0.9535809180714381 1103 92-93 0.8818204813953905 1103 94-95 0.910468713667651 1103 96-97 0.9693948209294447 1103 98-99 0.9791221862982908 1103 100-101 0.903386540608242 1103 102-103 0.8816263629966556 1103 104-105 0.9557188625644031 1103 106-107 1.0242002647381305 1103 108-109 1.0848485116760607 1103 110-111 0.9060418416728062 1103 112-113 1.003315754512684 1103 114-115 1.076094304197678 1103 116-117 1.0981315806297864 1103 118-119 1.141553040293072 1103 120-121 1.1386794515725676 1103 122-123 1.0024175281935577 1103 124-125 0.8994530645911318 1104 1 1.3011171885936292 1104 2 1.6777062508501075 1104 3 1.1812466971063564 1104 4 1.314633474206694 1104 5 1.0163015386986132 1104 6 0.9974539144015395 1104 7 0.8385832893385015 1104 8 1.0874688382552478 1104 9 1.548393883016562 1104 10-11 1.5748435277644788 1104 12-13 1.5248141779816144 1104 14-15 1.584609527626398 1104 16-17 1.372445489728733 1104 18-19 1.3974524538322441 1104 20-21 1.6596531765553237 1104 22-23 1.5217646604320478 1104 24-25 1.50540862772597 1104 26-27 1.7166446829208795 1104 28-29 1.7890504282029482 1104 30-31 1.793039094930002 1104 32-33 1.788145547238237 1104 34-35 1.6913897114276608 1104 36-37 1.4613154778486361 1104 38-39 1.6443557937453583 1104 40-41 1.716133226315673 1104 42-43 1.9208558301133678 1104 44-45 1.709449976410447 1104 46-47 1.5064458676624213 1104 48-49 1.347824751980479 1104 50-51 1.4085465618316029 1104 52-53 1.3564827634060883 1104 54-55 1.3724882561583769 1104 56-57 1.3550660355140067 1104 58-59 1.3047749567197897 1104 60-61 1.2418271350166457 1104 62-63 1.2014819869407134 1104 64-65 1.090735442337742 1104 66-67 1.1116648883017035 1104 68-69 0.9777722453318134 1104 70-71 1.019814392689348 1104 72-73 0.9521546425353584 1104 74-75 0.8714950574930285 1104 76-77 0.8728064922607999 1104 78-79 0.9396138689948543 1104 80-81 1.0832075803872279 1104 82-83 1.0877610286890302 1104 84-85 1.095911990804428 1104 86-87 0.9977146784061475 1104 88-89 1.021728349567212 1104 90-91 1.0614912590461607 1104 92-93 1.0541709744077536 1104 94-95 1.0297100622865365 1104 96-97 1.0095196861082378 1104 98-99 1.0465276841204911 1104 100-101 1.0568395811848212 1104 102-103 0.9925298989240474 1104 104-105 1.0246052313916572 1104 106-107 1.035681489713923 1104 108-109 1.018916537163232 1104 110-111 1.0275372911467677 1104 112-113 1.1294288202932599 1104 114-115 1.1703753503942202 1104 116-117 1.0670894883840525 1104 118-119 1.0188678847685786 1104 120-121 1.0289085018706885 1104 122-123 1.0743065618918912 1104 124-125 0.866743806222285 1105 1 1.3079036260329815 1105 2 1.684214577956471 1105 3 1.1816962698603284 1105 4 1.290238757586323 1105 5 1.0719726835908254 1105 6 0.8982628649113025 1105 7 0.9292668113090627 1105 8 1.0816059296667646 1105 9 1.3871560287258156 1105 10-11 1.2898558456372626 1105 12-13 1.4361084333595038 1105 14-15 1.7579751347420327 1105 16-17 1.3158843138688923 1105 18-19 1.4476605613794504 1105 20-21 1.3085396601750219 1105 22-23 1.3939264938475446 1105 24-25 1.123038587048633 1105 26-27 1.4141683012656259 1105 28-29 1.5684150232151026 1105 30-31 1.448952149070685 1105 32-33 1.59955441211806 1105 34-35 1.3374735517994978 1105 36-37 1.0569795118413552 1105 38-39 1.297742795881117 1105 40-41 1.3278581320386031 1105 42-43 1.4986941213411598 1105 44-45 1.2460293533061915 1105 46-47 0.9017565690987439 1105 48-49 1.0313742219660575 1105 50-51 1.1328977779449865 1105 52-53 0.9480461890772425 1105 54-55 0.9355682369508429 1105 56-57 0.9806388493175007 1105 58-59 0.9090437399956528 1105 60-61 0.9021845402924242 1105 62-63 0.8390018597547027 1105 64-65 0.5694688047960597 1105 66-67 0.5756151285204787 1105 68-69 0.4178120470492708 1105 70-71 0.7356153130553764 1105 72-73 0.5822786890921208 1105 74-75 0.6099045951349886 1105 76-77 0.642026603891324 1105 78-79 0.6659272041438271 1105 80-81 0.8107095061260026 1105 82-83 0.8152345591291024 1105 84-85 0.768287969176324 1105 86-87 0.7511804628891774 1105 88-89 0.7989570650121998 1105 90-91 0.6084965015427919 1105 92-93 0.7369929560615063 1105 94-95 0.6819639011439591 1105 96-97 0.6720597511346433 1105 98-99 0.7238546885870818 1105 100-101 0.7346843616537164 1105 102-103 0.6701783272656847 1105 104-105 0.7803824866717015 1105 106-107 0.7506772923512521 1105 108-109 0.7013473336525315 1105 110-111 0.7751887779230522 1105 112-113 0.7150671325857658 1105 114-115 0.7234922816889728 1105 116-117 0.7826391356747138 1105 118-119 0.8057302408756968 1105 120-121 0.7373220094013249 1105 122-123 0.8341164949023572 1105 124-125 0.758758238045214 1106 1 0.6219816721714935 1106 2 -1.4655197605613992 1106 3 0.09705503170756558 1106 4 0.5996281060060298 1106 5 0.652584384664209 1106 6 0.7580410193860558 1106 7 0.7294250250505563 1106 8 0.7510304900225648 1106 9 1.2627830476900463 1106 10-11 1.5186888533644805 1106 12-13 1.4465500163109972 1106 14-15 1.5513623714176106 1106 16-17 1.1850879718762997 1106 18-19 1.1656652988307599 1106 20-21 1.4526194939450363 1106 22-23 1.1248533238270753 1106 24-25 1.2578972722722455 1106 26-27 1.207275432639534 1106 28-29 1.3496619333996094 1106 30-31 1.4268352212635094 1106 32-33 1.5267436645797687 1106 34-35 1.4144111537396782 1106 36-37 1.1909056897986332 1106 38-39 1.505125326696497 1106 40-41 1.4529258896515707 1106 42-43 1.6948969687632385 1106 44-45 1.5623631261305846 1106 46-47 1.3977636731493774 1106 48-49 1.195740901486868 1106 50-51 1.2333602763369562 1106 52-53 1.2210157730663838 1106 54-55 1.2479197834191638 1106 56-57 1.1563679242705973 1106 58-59 1.2294783197450556 1106 60-61 1.2035907167491189 1106 62-63 1.201845718724094 1106 64-65 1.1004622646770983 1106 66-67 0.9733177258715919 1106 68-69 0.9118946754173898 1106 70-71 1.0636570850067564 1106 72-73 0.8456651177643302 1106 74-75 0.9136229977609389 1106 76-77 0.8677224474278518 1106 78-79 0.8806239500014037 1106 80-81 1.0044532970681956 1106 82-83 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-2.6236708939164366 2216 96-97 -2.627364442318555 2216 98-99 -2.7962017722598596 2216 100-101 -2.7641772727894853 2216 102-103 -2.4661029785794426 2216 104-105 -2.582581393683249 2216 106-107 -2.5775668434832575 2216 108-109 -2.6624425633716378 2216 110-111 -2.572227734071639 2216 112-113 -2.8464609147949176 2216 114-115 -2.7612108629142647 2216 116-117 -2.6678776168603164 2216 118-119 -2.753560867301534 2216 120-121 -2.918088746640745 2216 122-123 -2.7216847923272027 2216 124-125 -2.5635965894459396 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 269379.0 3 34917.0 4 20682.0 5 16993.0 6 17209.0 7 18954.0 8 20844.0 9 22183.0 10 23213.0 11 25607.0 12 27802.0 13 28770.0 14 28827.0 15 23724.0 16 18890.0 17 16643.0 18 14973.0 19 14009.0 20 13927.0 21 15010.0 22 18842.0 23 26851.0 24 39725.0 25 51902.0 26 57519.0 27 62193.0 28 71914.0 29 82848.0 30 96618.0 31 111995.0 32 135461.0 33 171740.0 34 225993.0 35 311508.0 36 467013.0 37 579081.0 38 166600.0 39 267.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.10485637912788 18.914529605273977 13.982913875321945 22.99770014027619 2 13.319917338164448 20.23222614976295 42.55852022390971 23.889336288162895 3 18.960572135767016 26.291907164117905 28.27708299350676 26.470437706608323 4 11.933617831192617 17.069692000143647 37.46187409473419 33.53481607392954 5 13.258692549961337 39.58497042238469 33.271546786096714 13.88479024155726 6 31.405382609111793 37.42495573315467 17.624545367534456 13.545116290199081 7 28.560315752734528 33.666235099647714 20.241240879296118 17.532208268321636 8 25.773443104220046 36.88491967972959 19.418688340964728 17.92294887508563 9 26.03151137631731 17.79475982532751 19.995389191691217 36.17833960666396 10-11 24.156970180662533 28.395740056223655 27.10008261855853 20.34720714455528 12-13 25.02280424252325 25.467335640934852 27.530919683181327 21.97894043336057 14-15 22.030971650205387 26.73361400049062 25.511795509913465 25.72361883939053 16-17 22.062122001041377 30.212656826413852 25.784856906639153 21.940364265905625 18-19 22.06464073284013 28.552183317662216 27.903787825561803 21.47938812393585 20-21 23.036805501266002 27.52939446043775 27.76423922985385 21.66956080844239 22-23 22.590525366479124 27.492363024840206 27.51444566444674 22.402665944233934 24-25 22.45539328893093 27.501611570297353 27.773425773882636 22.269569366889076 26-27 22.41008670637817 27.67891118040814 27.667139527147494 22.24386258606619 28-29 22.107159495980618 27.681292922142685 27.961273796393876 22.250273785482815 30-31 22.22212259566242 27.41535129719758 27.983446847835573 22.379079259304426 32-33 22.130754811444763 27.390212322626585 27.832237812344573 22.64679505358408 34-35 22.650095295991413 27.318119448601287 27.60360394286604 22.428181312541263 36-37 22.614297470729348 27.25099230084233 27.512881119213432 22.621829109214886 38-39 22.37612869326824 27.33863451377901 27.877776374294534 22.40746041865822 40-41 22.51429659867363 27.22574145781126 27.958150126508585 22.301811817006527 42-43 22.22951313134124 27.228224256196366 27.922690830195673 22.619571782266725 44-45 22.428124852871832 27.49266694516706 27.499093877300893 22.580114324660215 46-47 22.536094666951406 27.61228011473061 27.08863078702372 22.762994431294263 48-49 22.568490207785203 27.43864594020431 27.18843988860137 22.80442396340912 50-51 22.620052524380554 27.600766882260135 27.168498882377314 22.610681710981993 52-53 22.806295301066356 27.625234540086176 26.942063665749433 22.626406493098035 54-55 22.5427120641824 27.545987576221233 27.097773772796497 22.813526586799874 56-57 22.5987863751177 27.86660588578176 27.039876246132692 22.49473149296785 58-59 22.678320230022944 27.33400755087787 27.167096868863194 22.82057535023599 60-61 22.632790246556016 27.41571992623345 27.428392947344193 22.523096879866337 62-63 22.63886367253255 27.545824657038875 27.235816640105437 22.579495030323134 64-65 22.492772004462985 27.449617707061208 27.144599209160386 22.913011079315417 66-67 22.394733484393157 27.829181877482572 26.956768064801732 22.81931657332253 68-69 22.431975114131202 27.52802013460422 27.196133845214852 22.84387090604972 70-71 22.442930489819954 27.52090403381401 27.277973035989017 22.758192440377016 72-73 22.730633788615233 27.43713545555228 26.969410159129076 22.86282059670341 74-75 22.50910861774578 27.734176250819736 26.914333431428055 22.842381700006424 76-77 22.684422968741945 27.544515889006675 26.987756309545958 22.783304832705422 78-79 22.73488511368496 27.60685860258483 26.930080230803643 22.728176052926568 80-81 22.38938744732584 27.916962860339495 27.046575617590896 22.647074074743767 82-83 22.571256589474892 27.952105673090315 26.772246706831655 22.704391030603137 84-85 22.607667638348918 27.57596282534658 26.932886386392447 22.88348314991206 86-87 22.634340342567967 27.400983569560903 27.23096248880015 22.73371359907098 88-89 22.70105455931801 27.41779502157376 27.089155941559934 22.791994477548304 90-91 22.552288087622706 27.761311925766485 27.127058663196635 22.559341323414174 92-93 22.649145169509126 27.572762579629018 27.16670875691314 22.61138349394872 94-95 22.524885511699928 27.464383078449895 27.144996592254255 22.865734817595925 96-97 22.719213507057443 27.570771691801504 26.940467849960903 22.769546951180146 98-99 22.552794109361997 27.46008004240475 26.884247552528855 23.102878295704397 100-101 22.331558274314524 27.608833987780823 27.15728240112728 22.90232533677737 102-103 22.603672963645522 27.383174077910354 27.15386420467199 22.859288753772134 104-105 22.485705180468596 27.342739056400724 27.232970261127814 22.938585502002866 106-107 22.434197727614347 27.53233298001315 27.124306446036293 22.909162846336205 108-109 22.483103034505227 27.65743727986874 26.73608755489739 23.123372130728644 110-111 22.281932676566473 27.681868555595955 27.10282272702962 22.93337604080795 112-113 22.42521631496283 27.551584407575902 27.153297778038038 22.86990149942323 114-115 22.23066387166077 27.709770714610187 26.938624544595335 23.1209408691337 116-117 22.208332598849783 27.661267938702544 27.078481384800764 23.051918077646913 118-119 22.28206059621166 27.74557166823598 26.85021161833659 23.122156117215766 120-121 22.175827891620376 27.831012521540583 26.966551574474924 23.02660801236412 122-123 22.339621998846106 27.75249251476207 26.902920624657707 23.004964861734116 124-125 22.33031217596978 27.733334249574952 26.876060742636742 23.060292831818522 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4418.0 1 3308.5 2 1737.5 3 1120.0 4 892.5 5 823.0 6 859.0 7 1021.5 8 1288.0 9 1420.5 10 1428.5 11 1464.0 12 1532.0 13 1691.0 14 1883.5 15 2003.5 16 2199.0 17 2458.5 18 2727.5 19 3094.5 20 3632.0 21 4487.0 22 5664.5 23 7292.0 24 9207.0 25 12068.0 26 16423.0 27 21533.5 28 26184.5 29 32412.5 30 39688.5 31 44753.5 32 50740.5 33 58703.0 34 67843.5 35 76202.0 36 84780.5 37 93630.0 38 99118.0 39 100917.0 40 102184.5 41 107781.0 42 114119.0 43 119617.0 44 124920.5 45 128178.0 46 128408.5 47 128307.0 48 132390.5 49 131502.5 50 125695.0 51 120175.0 52 114508.0 53 110862.5 54 107287.5 55 99733.5 56 89002.0 57 79935.0 58 72827.5 59 64938.0 60 55028.0 61 45016.0 62 35843.5 63 27636.5 64 21497.5 65 16930.5 66 12803.0 67 10094.5 68 7844.0 69 5744.0 70 4413.0 71 3436.0 72 2742.0 73 2229.5 74 1733.0 75 1388.5 76 1019.0 77 657.0 78 452.5 79 340.5 80 251.0 81 164.0 82 118.0 83 106.5 84 89.0 85 62.0 86 44.0 87 34.5 88 24.0 89 15.5 90 10.0 91 9.0 92 7.0 93 7.5 94 8.0 95 5.0 96 3.0 97 2.5 98 2.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19426220652498966 2 0.2342845784638453 3 0.2823651460950879 4 0.270576304248818 5 0.30122729304911977 6 0.21685499963290442 7 0.20882664910974844 8 0.18883038572493618 9 0.1883230178480081 10-11 0.13506431335517602 12-13 0.15834354535540524 14-15 0.17481807877095204 16-17 0.15065841427840648 18-19 0.1740271817863289 20-21 0.168640128740122 22-23 0.1901137280018719 24-25 0.18092141587870447 26-27 0.2342845784638453 28-29 0.16108930092466303 30-31 0.14319712197064072 32-33 0.17974253169407747 34-35 0.14131687630908374 36-37 0.14148102473985458 38-39 0.17231108455554275 40-41 0.19060617329418442 42-43 0.2556089518794399 44-45 0.1590299842477197 46-47 0.1455101225860481 48-49 0.1487334008630029 50-51 0.15316540849381577 52-53 0.1725647684940068 54-55 0.15959704246310988 56-57 0.15746311286308887 58-59 0.1350493907705605 60-61 0.14731575532452743 62-63 0.13679533317057768 64-65 0.13237824812438034 66-67 0.12084309021657445 68-69 0.12381268455506522 70-71 0.12542432369354262 72-73 0.10353289206255786 74-75 0.10508484086257314 76-77 0.125603394708929 78-79 0.13146797046283293 80-81 0.14031706313984313 82-83 0.14206300553986032 84-85 0.13034877661666805 86-87 0.15395630547843897 88-89 0.1495392204322416 90-91 0.138600965909057 92-93 0.1385114304013638 94-95 0.15835846794002076 96-97 0.14737544566298955 98-99 0.14249576049371074 100-101 0.12429020726276224 102-103 0.16029840394003986 104-105 0.12076847729349678 106-107 0.12758809846279473 108-109 0.1376160753244319 110-111 0.11421746264727846 112-113 0.10562205390873228 114-115 0.10342843397024915 116-117 0.1075321447395203 118-119 0.09222157292398495 120-121 0.08580486153930639 122-123 0.11057635200108874 124-125 0.10802459003183286 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3350626.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.04372156359648 #Duplication Level Percentage of deduplicated Percentage of total 1 77.26185383955126 41.75518116391996 2 11.5882232255474 12.52541418836571 3 4.39921766437196 7.132502836529203 4 2.1811966252896506 4.7151993237044065 5 1.1885707780605177 3.2117394094064915 6 0.7479772018725352 2.425408298035037 7 0.5034600613234681 1.9046198760789703 8 0.3612511641920711 1.5618685865697093 9 0.26293046584751295 1.2788766798164735 >10 1.3508611548014078 13.1797328217196 >50 0.09650104188703744 3.619502542252638 >100 0.055415200911175175 5.409138513866323 >500 0.0017328929628330676 0.5822031718906646 >1k 8.086833811423811E-4 0.6986125878448124 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 2.9845169231063092E-5 0.0 0.0 0.0 0.0 6 5.9690338462126184E-5 0.0 0.0 0.0 0.0 7 1.1938067692425237E-4 0.0 0.0 0.0 0.0 8 1.4922584615531545E-4 0.0 0.0 0.0 0.0 9 1.4922584615531545E-4 0.0 0.0 0.0 0.0 10-11 1.7907101538637855E-4 0.0 0.0 0.0 0.0 12-13 2.0891618461744163E-4 0.0 0.0 0.0 0.0 14-15 2.984516923106309E-4 0.0 0.0 1.4922584615531546E-5 0.0 16-17 3.1337427692616245E-4 0.0 0.0 2.9845169231063092E-5 0.0 18-19 3.5814203077275705E-4 0.0 0.0 2.9845169231063092E-5 0.0 20-21 3.8798720000382016E-4 0.0 0.0 2.9845169231063092E-5 0.0 22-23 3.8798720000382016E-4 0.0 0.0 1.1938067692425237E-4 0.0 24-25 4.1783236923488326E-4 0.0 0.0 2.6860652307956784E-4 0.0 26-27 4.1783236923488326E-4 0.0 0.0 5.521356307746671E-4 0.0 28-29 5.521356307746671E-4 0.0 0.0 8.356647384697665E-4 0.0 30-31 5.969033846212618E-4 0.0 0.0 0.002342845784638453 0.0 32-33 5.969033846212618E-4 0.0 0.0 0.006088414523136871 0.0 34-35 5.969033846212618E-4 0.0 0.0 0.010087667200099324 0.0 36-37 6.56593723083388E-4 0.0 0.0 0.017444501415556375 0.0 38-39 7.759744000076403E-4 0.0 0.0 0.030561453292608606 0.0 40-41 7.908969846231719E-4 0.0 0.0 0.04655846400045842 0.0 42-43 8.953550769318927E-4 0.0 0.0 0.06130197760060359 0.0 44-45 9.998131692406137E-4 0.0 0.0 0.07944784049308995 0.0 46-47 0.0011341164307803976 0.0 0.0 0.10216001427792895 0.0 48-49 0.0011938067692425236 0.0 0.0 0.12878190523203725 0.0 50-51 0.0011938067692425236 0.0 0.0 0.15746311286308887 0.0 52-53 0.0011938067692425236 0.0 0.0 0.1864875399402977 0.0 54-55 0.0011938067692425236 0.0 0.0 0.21700422547905973 0.0 56-57 0.0012534971077046498 0.0 0.0 0.25386300947942264 0.0 58-59 0.0012534971077046498 0.0 0.0 0.2964072982183031 0.0 60-61 0.0012534971077046498 0.0 0.0 0.34483108529570294 0.0 62-63 0.0012534971077046498 0.0 0.0 0.39464267274234727 0.0 64-65 0.0012534971077046498 0.0 0.0 0.44560329920438746 0.0 66-67 0.0013728777846289022 0.0 0.0 0.5048758052972788 0.0 68-69 0.0014027229538599653 0.0 0.0 0.5760266887441332 0.0 70-71 0.001477335876937623 0.0 0.0 0.6552208452987591 0.0 72-73 0.0014922584615531546 0.0 0.0 0.7396229838842054 0.0 74-75 0.0014922584615531546 0.0 0.0 0.8267410328696787 0.0 76-77 0.0014922584615531546 0.0 0.0 0.9213352967475332 2.9845169231063092E-5 78-79 0.0015071810461686863 0.0 0.0 1.0325234747178587 2.9845169231063092E-5 80-81 0.0017011746461705962 0.0 0.0 1.1628424061652956 2.9845169231063092E-5 82-83 0.0017011746461705962 2.9845169231063092E-5 0.0 1.3060992184743985 2.9845169231063092E-5 84-85 0.0017459424000171907 2.9845169231063092E-5 0.0 1.448669591891187 2.9845169231063092E-5 86-87 0.0018205553230948486 2.9845169231063092E-5 0.0 1.601924535892696 2.9845169231063092E-5 88-89 0.0018504004923259117 2.9845169231063092E-5 0.0 1.7814730739867715 2.9845169231063092E-5 90-91 0.0020145489230967584 2.9845169231063092E-5 0.0 1.9879121095580348 2.9845169231063092E-5 92-93 0.002059316676943353 2.9845169231063092E-5 0.0 2.2106913752833055 2.9845169231063092E-5 94-95 0.002059316676943353 2.9845169231063092E-5 0.0 2.434455531593201 2.9845169231063092E-5 96-97 0.002059316676943353 2.9845169231063092E-5 0.0 2.662726308456987 2.9845169231063092E-5 98-99 0.0023130006154073895 2.9845169231063092E-5 0.0 2.914947833628701 2.9845169231063092E-5 100-101 0.0024771490461782367 2.9845169231063092E-5 0.0 3.215608068462431 2.9845169231063092E-5 102-103 0.0026114523077180206 2.9845169231063092E-5 0.0 3.540845800157941 2.9845169231063092E-5 104-105 0.003118820184646093 2.9845169231063092E-5 0.0 3.8636362279765035 2.9845169231063092E-5 106-107 0.0032680460308014084 2.9845169231063092E-5 0.0 4.188321824041239 2.9845169231063092E-5 108-109 0.003312813784648003 2.9845169231063092E-5 0.0 4.545478964229371 2.9845169231063092E-5 110-111 0.003357581538494598 2.9845169231063092E-5 0.0 4.950089923494893 2.9845169231063092E-5 112-113 0.0034023492923411923 2.9845169231063092E-5 0.0 5.395693222699281 2.9845169231063092E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 11140 0.0 38.073902 1 GTACATG 16030 0.0 36.49308 1 TACATGG 16670 0.0 34.12718 2 CGCATAG 510 0.0 32.66586 8 TCAACGC 12755 0.0 32.505352 4 ATCAACG 12865 0.0 32.41312 3 ACATGGG 16965 0.0 32.164307 3 CAACGCA 13060 0.0 31.79327 5 TATCAAC 13735 0.0 31.053957 2 AACGCAG 13085 0.0 30.29351 6 GGTATCA 7745 0.0 27.689407 1 CATGGGG 7085 0.0 25.980333 4 CATGGGA 10245 0.0 25.932774 4 ACGCAGA 15040 0.0 25.916187 7 ACGCATA 690 0.0 25.010742 7 CGCAGAG 14735 0.0 24.873495 8 GTTATCA 2320 0.0 24.136398 1 AACGCAT 730 0.0 23.644178 6 AGTACAT 12470 0.0 21.974752 2 GAGTACA 14645 0.0 21.111086 1 >>END_MODULE