FastQCFastQC Report
Thu 26 May 2016
SRR1364595_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364595_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3350626
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89260.2663979805564692No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75700.22592793107914758No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46680.13931724997060252No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95200.043.6869471
GTATCAA150350.042.4442861
ATCAACG179750.035.185873
TCAACGC182350.034.6867684
TATCAAC183300.034.3723952
CAACGCA185550.033.640185
AACGCAG188600.033.2771876
GTACATG160150.031.7734321
TACATGG166500.029.8289782
ACATGGG170150.028.4640313
ACGCAGA219850.028.0595667
CGCAGAG219700.027.9703128
GAGTACT120650.025.0231112-13
GCAGAGT238100.024.734189
AGTACTT126850.023.37757912-13
GTACTTT129450.023.2990414-15
CATGGGA102700.023.1173924
CATGGGG71950.022.9079024
GTAAGGT10600.022.4538334
TAAGGTG12150.022.0594255
Produced by FastQC (version 0.11.5)