##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364595_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3350626 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.57912222969678 31.0 30.0 33.0 26.0 34.0 2 29.709900776750374 31.0 30.0 34.0 26.0 34.0 3 29.67940826579869 31.0 30.0 34.0 25.0 34.0 4 33.35992020595555 35.0 33.0 37.0 28.0 37.0 5 32.73845335170204 35.0 33.0 37.0 26.0 37.0 6 32.72184063515295 35.0 33.0 37.0 25.0 37.0 7 32.52358932330854 35.0 33.0 37.0 25.0 37.0 8 32.449510628760116 35.0 33.0 37.0 23.0 37.0 9 33.74427375660548 38.0 33.0 39.0 23.0 39.0 10-11 33.51772653826479 37.0 33.0 39.0 20.5 39.0 12-13 33.385838944722565 37.0 33.0 39.0 18.5 39.0 14-15 34.366382878900836 38.0 33.0 41.0 19.0 41.0 16-17 34.279637596079056 38.0 33.0 41.0 18.5 41.0 18-19 34.31418845314279 38.5 33.0 41.0 18.0 41.0 20-21 34.3387198392181 39.0 33.0 41.0 18.0 41.0 22-23 34.331021427040795 39.0 33.0 41.0 18.5 41.0 24-25 34.323520291432104 39.0 33.0 41.0 19.0 41.0 26-27 34.22574706935361 39.0 33.0 41.0 18.5 41.0 28-29 33.9986259284086 38.5 33.0 41.0 17.5 41.0 30-31 33.93573365096552 38.0 33.0 41.0 17.5 41.0 32-33 33.76544577043215 38.0 33.0 41.0 16.5 41.0 34-35 33.63288322838777 38.0 33.0 40.5 16.0 41.0 36-37 33.4674857772846 38.0 32.5 40.0 15.0 41.0 38-39 33.33571890745192 38.0 32.0 40.0 13.5 41.0 40-41 33.16198480522745 38.0 32.0 40.0 9.0 41.0 42-43 32.989069952898355 38.0 32.0 40.0 8.5 41.0 44-45 32.78612578664405 38.0 31.5 40.0 7.5 41.0 46-47 32.5251297817184 38.0 31.0 40.0 2.0 41.0 48-49 32.27384837937747 38.0 31.0 40.0 2.0 41.0 50-51 32.077360618582915 38.0 30.5 40.0 2.0 41.0 52-53 31.904551268927058 37.5 30.0 40.0 2.0 41.0 54-55 31.684902164550742 37.0 30.0 40.0 2.0 41.0 56-57 31.46048350367961 37.0 30.0 40.0 2.0 41.0 58-59 31.273643641516543 36.5 29.5 40.0 2.0 41.0 60-61 31.05296846022206 36.0 29.0 40.0 2.0 41.0 62-63 30.79061211248286 36.0 29.0 39.5 2.0 41.0 64-65 30.520419169432817 35.0 29.0 39.0 2.0 41.0 66-67 30.247503750045514 35.0 28.5 39.0 2.0 41.0 68-69 29.93531984769413 35.0 28.0 38.5 2.0 40.0 70-71 29.67806075640791 35.0 28.0 38.0 2.0 40.0 72-73 29.518546385063566 35.0 28.0 37.0 2.0 40.0 74-75 29.23126588882197 35.0 28.0 37.0 2.0 39.0 76-77 28.9334290069975 34.0 28.0 36.5 2.0 39.0 78-79 28.631959221948378 34.0 28.0 36.0 2.0 39.0 80-81 28.35757750939675 34.0 27.5 35.5 2.0 37.0 82-83 28.10875863196907 34.0 27.0 35.0 2.0 37.0 84-85 28.093298237403996 34.0 28.0 35.0 2.0 36.5 86-87 27.94965179641058 34.0 27.5 35.0 2.0 36.0 88-89 27.81282482736062 34.0 27.0 35.0 2.0 36.0 90-91 27.66873697631428 34.0 27.0 35.0 2.0 36.0 92-93 27.541652813533947 34.0 27.0 35.0 2.0 35.0 94-95 27.458650861062978 34.0 27.0 35.0 2.0 35.0 96-97 27.382609398960074 34.0 27.0 35.0 2.0 35.0 98-99 27.29950701749464 34.0 27.0 35.0 2.0 35.0 100-101 27.204572817139244 34.0 26.0 35.0 2.0 35.0 102-103 26.040338283055167 32.0 24.5 34.0 2.0 35.0 104-105 26.962431796327017 34.0 25.5 35.0 2.0 35.0 106-107 27.137852299838897 34.0 26.0 35.0 2.0 35.0 108-109 27.15015850769379 34.0 26.5 35.0 2.0 35.0 110-111 27.09747238277265 34.0 26.0 35.0 2.0 35.0 112-113 27.050772900347575 34.0 26.0 35.0 2.0 35.0 114-115 26.974711889658828 34.0 26.0 35.0 2.0 35.0 116-117 26.860263723853393 34.0 25.0 35.0 2.0 35.0 118-119 26.761056590619184 34.0 25.0 35.0 2.0 35.0 120-121 26.62975321626466 34.0 25.0 35.0 2.0 35.0 122-123 26.45100721477121 34.0 25.0 35.0 2.0 35.0 124-125 25.64952504397686 33.0 22.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6330082889204149 1101 2 0.7295504023650068 1101 3 0.5589641682196032 1101 4 0.6107384225951051 1101 5 0.868172359464964 1101 6 0.9627725186791167 1101 7 0.9545838744069144 1101 8 0.9313489260806662 1101 9 1.1014914506764342 1101 10-11 1.151606005819474 1101 12-13 1.0871187618731994 1101 14-15 1.102564921208021 1101 16-17 1.1807697181374692 1101 18-19 1.1778965128486902 1101 20-21 1.2210216536368819 1101 22-23 1.1302853086604614 1101 24-25 1.142903038764679 1101 26-27 1.2476179391271316 1101 28-29 1.2995303886750236 1101 30-31 1.2471208446543685 1101 32-33 1.329313795433606 1101 34-35 1.3490635240231228 1101 36-37 1.3150352947453925 1101 38-39 1.312787231163476 1101 40-41 1.3492518022457887 1101 42-43 1.3671835090544775 1101 44-45 1.2591656320278162 1101 46-47 1.3199604286529336 1101 48-49 1.3649634119509102 1101 50-51 1.3107281991915443 1101 52-53 1.3569466035711564 1101 54-55 1.1485191454877892 1101 56-57 1.2429148407725386 1101 58-59 1.2768415605866252 1101 60-61 1.2824788536016385 1101 62-63 1.286507619039515 1101 64-65 1.3211930748796519 1101 66-67 1.2278565425082562 1101 68-69 1.2630737477233112 1101 70-71 1.222041924462978 1101 72-73 1.1856515965284267 1101 74-75 1.2099436533955803 1101 76-77 1.1741447356005779 1101 78-79 1.1923753184754275 1101 80-81 1.1778224662913033 1101 82-83 1.0838780416411389 1101 84-85 1.1899550492947881 1101 86-87 1.107539333489683 1101 88-89 1.0267538533561904 1101 90-91 1.0766763772240608 1101 92-93 1.1461440829005234 1101 94-95 1.0861050562381287 1101 96-97 1.1539480979661647 1101 98-99 1.1799690972581374 1101 100-101 1.2428159615970706 1101 102-103 1.1845147238574576 1101 104-105 1.1477798622368418 1101 106-107 1.1285232001766268 1101 108-109 1.0992227699802015 1101 110-111 1.1569473040316147 1101 112-113 1.1200981085696213 1101 114-115 1.0284447330110353 1101 116-117 1.088470442566539 1101 118-119 1.0829639194519665 1101 120-121 1.0716741973783002 1101 122-123 1.0814100623252791 1101 124-125 1.0319684741638362 1102 1 0.5286323804603974 1102 2 -0.3937658326873894 1102 3 0.12410794356889454 1102 4 0.4228926774184245 1102 5 0.772155488546808 1102 6 0.6297288264533165 1102 7 0.5930122875968706 1102 8 0.5219085278079376 1102 9 0.8230782285772875 1102 10-11 0.9947299077118998 1102 12-13 1.0083026431808335 1102 14-15 1.1192140079746693 1102 16-17 1.2169079040267903 1102 18-19 1.1577100571558177 1102 20-21 1.1219566000244825 1102 22-23 1.1875128252996205 1102 24-25 1.1977199019040725 1102 26-27 1.2092765870236164 1102 28-29 1.2896413132100832 1102 30-31 1.2031063787243994 1102 32-33 1.0989917787021852 1102 34-35 1.0667824560566572 1102 36-37 1.038461814674136 1102 38-39 0.8533374951593089 1102 40-41 0.9048107418137903 1102 42-43 0.7886546340887932 1102 44-45 0.6432640941468506 1102 46-47 0.6930544305185578 1102 48-49 0.791628951446782 1102 50-51 0.6824534037376822 1102 52-53 0.7932344663846145 1102 54-55 0.7658777150180427 1102 56-57 0.5767900089642062 1102 58-59 0.6696615603037586 1102 60-61 0.6052550993771604 1102 62-63 0.7487015793112413 1102 64-65 0.7142739203477504 1102 66-67 0.7491861068910275 1102 68-69 0.4641895182356244 1102 70-71 0.43447925237389384 1102 72-73 0.44562697770394877 1102 74-75 0.5576019399973973 1102 76-77 0.6568196276139773 1102 78-79 0.6417522378533569 1102 80-81 0.5290981193378457 1102 82-83 0.4533981111742911 1102 84-85 0.4930246637702318 1102 86-87 0.5555667015608776 1102 88-89 0.6335625944371301 1102 90-91 0.5704812102795671 1102 92-93 0.613065651420829 1102 94-95 0.40221370371675036 1102 96-97 0.4210030787217569 1102 98-99 0.5275451764652423 1102 100-101 0.30435506724490224 1102 102-103 0.31656651917695555 1102 104-105 0.3591995220669837 1102 106-107 0.6110402668496349 1102 108-109 0.6116751813006793 1102 110-111 0.4813343888400752 1102 112-113 0.5398669056962362 1102 114-115 0.6317924463436455 1102 116-117 0.5910643952523671 1102 118-119 0.5750973628666003 1102 120-121 0.5747562916273488 1102 122-123 0.5084604818963925 1102 124-125 0.4144939062227557 1103 1 0.27472727972262234 1103 2 -1.0307657623732922 1103 3 -0.09100402246459183 1103 4 0.15281521214140525 1103 5 0.3657754089949208 1103 6 0.458027749197349 1103 7 0.46001011763702593 1103 8 0.5660870053553779 1103 9 0.6220126237322461 1103 10-11 0.8126799682447654 1103 12-13 0.7711681878800221 1103 14-15 0.8569623468957914 1103 16-17 0.8954362112356833 1103 18-19 0.9692183116478859 1103 20-21 0.9111557925285965 1103 22-23 0.8907798602339909 1103 24-25 0.7947056107064299 1103 26-27 0.7628881325168706 1103 28-29 0.9348787471317408 1103 30-31 0.8373044994070185 1103 32-33 0.7978977915677916 1103 34-35 0.8462562638110711 1103 36-37 0.8964403951913908 1103 38-39 0.8888659129316139 1103 40-41 0.8382584082046094 1103 42-43 0.8580663601173697 1103 44-45 0.9438551684606935 1103 46-47 0.9493640207992087 1103 48-49 0.9739626934383665 1103 50-51 1.0045875196894585 1103 52-53 0.9050144078666555 1103 54-55 0.8871722211731381 1103 56-57 0.834799236813577 1103 58-59 0.894355940447511 1103 60-61 0.829633941954981 1103 62-63 0.8552214071125874 1103 64-65 0.7926440737978808 1103 66-67 0.7768779655859284 1103 68-69 0.7667242110726029 1103 70-71 0.8420411668695067 1103 72-73 0.7986302858885175 1103 74-75 0.6913671971583781 1103 76-77 0.5969476424531024 1103 78-79 0.632323162249083 1103 80-81 0.7768629172980681 1103 82-83 0.7037361362150705 1103 84-85 0.7109388869269679 1103 86-87 0.7141013622961694 1103 88-89 0.7470422345951668 1103 90-91 0.7432772483124062 1103 92-93 0.7467998457202683 1103 94-95 0.6506809704676577 1103 96-97 0.6564852953251865 1103 98-99 0.6794224447161312 1103 100-101 0.7429148251427939 1103 102-103 0.7736448551918116 1103 104-105 0.7675804165454032 1103 106-107 0.7888656164096908 1103 108-109 0.7253003586034197 1103 110-111 0.8273861748873941 1103 112-113 0.7665169017568552 1103 114-115 0.7604274224005962 1103 116-117 0.7859228663371027 1103 118-119 0.8231104192900389 1103 120-121 0.7696270452730722 1103 122-123 0.8013907212682305 1103 124-125 0.7318311423279624 1104 1 0.36055200911119556 1104 2 -0.736864541991114 1104 3 0.056778158680653945 1104 4 0.2929721268876335 1104 5 0.6587129000055043 1104 6 0.5826055870415985 1104 7 0.6302595500825916 1104 8 0.6606811995305861 1104 9 0.8037966970993295 1104 10-11 0.8812363714792539 1104 12-13 0.8120790162452138 1104 14-15 0.9202318528455393 1104 16-17 1.0649112125730866 1104 18-19 0.9363424587946341 1104 20-21 0.8716575836845806 1104 22-23 0.8966803802025893 1104 24-25 0.863193181848942 1104 26-27 0.6715050615436624 1104 28-29 0.996469975320494 1104 30-31 0.9307456459262369 1104 32-33 0.9715037795353538 1104 34-35 0.8950690884110415 1104 36-37 0.8671020674003955 1104 38-39 0.8868134632779459 1104 40-41 0.9300347911170164 1104 42-43 0.8551604565902551 1104 44-45 0.9312642011852077 1104 46-47 1.0596265653778971 1104 48-49 0.9682026806313573 1104 50-51 0.8105294710516375 1104 52-53 0.6983615479272736 1104 54-55 0.863940449144387 1104 56-57 0.8888806595324716 1104 58-59 0.8164878244975924 1104 60-61 0.8747880745873289 1104 62-63 0.7905815459075569 1104 64-65 0.813045221143561 1104 66-67 0.8248358747817051 1104 68-69 0.7940753375484313 1104 70-71 0.911926662141834 1104 72-73 0.8428455551929765 1104 74-75 0.8783657519647363 1104 76-77 0.816135991562426 1104 78-79 0.8189651435946637 1104 80-81 0.8110696840973439 1104 82-83 0.8731268492722997 1104 84-85 0.8846092623313524 1104 86-87 0.837865247639126 1104 88-89 0.7952355703673213 1104 90-91 0.8393949781779568 1104 92-93 0.8672291385444026 1104 94-95 0.8002346269853469 1104 96-97 0.7856054906529693 1104 98-99 0.8147067761134643 1104 100-101 0.9261426706590719 1104 102-103 0.8401188574027678 1104 104-105 0.7753633280556009 1104 106-107 0.8051925165518234 1104 108-109 0.7063372373887873 1104 110-111 0.5716929320125352 1104 112-113 0.6700782357556285 1104 114-115 0.7660242242669995 1104 116-117 0.8347939719783071 1104 118-119 0.6626539035537178 1104 120-121 0.6946869159792541 1104 122-123 0.7306548953936414 1104 124-125 0.721555119632832 1105 1 0.5843840080342773 1105 2 0.36028469466224067 1105 3 0.5510373226108811 1105 4 0.5042502097991033 1105 5 0.7197557810353601 1105 6 0.5298743098654626 1105 7 0.7035225368224189 1105 8 0.7050456402966674 1105 9 0.6578993931959403 1105 10-11 0.7656598713505645 1105 12-13 0.8706503377569632 1105 14-15 0.9711339118903126 1105 16-17 1.0542740376505648 1105 18-19 0.9977025959396499 1105 20-21 1.090147994564937 1105 22-23 0.9534296505719482 1105 24-25 1.0460037508517743 1105 26-27 0.9714985489319403 1105 28-29 1.186329901195137 1105 30-31 1.0557701850732641 1105 32-33 1.0591873713061517 1105 34-35 1.082392796062706 1105 36-37 1.1443550103841957 1105 38-39 1.1060595630337033 1105 40-41 0.9892046341101022 1105 42-43 1.101674923936514 1105 44-45 1.0835127427029576 1105 46-47 1.1559877838034183 1105 48-49 1.1526683177237018 1105 50-51 1.1353006415580467 1105 52-53 1.0323005827145941 1105 54-55 1.0561456942585252 1105 56-57 1.0062924931037678 1105 58-59 0.8518528641746741 1105 60-61 1.0536707441224564 1105 62-63 1.1568302628607263 1105 64-65 1.0904685748706129 1105 66-67 1.0040666885964171 1105 68-69 0.9919390762612004 1105 70-71 0.9189299399486615 1105 72-73 0.911851817894906 1105 74-75 0.9686150089349468 1105 76-77 0.9752061043459008 1105 78-79 0.9678959145320896 1105 80-81 0.7916819272464792 1105 82-83 0.8732646969569551 1105 84-85 0.654786984961401 1105 86-87 0.8012024765945078 1105 88-89 0.872080532503805 1105 90-91 0.8445897597998027 1105 92-93 0.7646758364009756 1105 94-95 0.842857684186022 1105 96-97 0.9355755139425668 1105 98-99 1.0031214630811967 1105 100-101 0.9861608670208604 1105 102-103 0.5660113267046825 1105 104-105 0.59148931811281 1105 106-107 0.8896837600493406 1105 108-109 0.8740209023875138 1105 110-111 0.8029381683019317 1105 112-113 0.6585432396600801 1105 114-115 0.7878823331541263 1105 116-117 0.5561459590104185 1105 118-119 0.3982884142259877 1105 120-121 0.4538451593564865 1105 122-123 0.5540054050911998 1105 124-125 0.4197326088683262 1106 1 0.4028911124646086 1106 2 0.4590041763979471 1106 3 0.6086471271545619 1106 4 0.35059735872430053 1106 5 0.5858654468363582 1106 6 0.6682464048313719 1106 7 0.6283566569211843 1106 8 0.6517565274281694 1106 9 0.6641004393172878 1106 10-11 0.8321904051446154 1106 12-13 0.7616639354511818 1106 14-15 0.8742123135806779 1106 16-17 0.9883345461671809 1106 18-19 0.8985462683999899 1106 20-21 0.968189796892382 1106 22-23 0.8561078475883761 1106 24-25 0.8933059233024778 1106 26-27 0.9377049852862598 1106 28-29 1.0501216453144409 1106 30-31 0.9616411344817735 1106 32-33 0.8894951978059566 1106 34-35 0.9298959061922005 1106 36-37 1.0379820888855207 1106 38-39 1.1205190262491271 1106 40-41 1.1387301751420154 1106 42-43 1.0408531161687193 1106 44-45 1.0144555656814163 1106 46-47 0.8840648831199474 1106 48-49 0.8685283530115839 1106 50-51 0.9554420922673401 1106 52-53 0.9385325896415502 1106 54-55 0.8775897846024137 1106 56-57 0.7259166166213582 1106 58-59 0.8425331452930109 1106 60-61 0.9106703998097423 1106 62-63 1.031402169160888 1106 64-65 0.9132384286296009 1106 66-67 0.8131477958830153 1106 68-69 0.8627367183494279 1106 70-71 0.8862721374231661 1106 72-73 0.9493632222093105 1106 74-75 1.0166115099158688 1106 76-77 1.0222987275030064 1106 78-79 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scores pass #Quality Count 2 134955.0 3 43965.0 4 20384.0 5 15673.0 6 16160.0 7 18981.0 8 21247.0 9 23030.0 10 24695.0 11 27218.0 12 30539.0 13 33265.0 14 35782.0 15 29593.0 16 19839.0 17 17035.0 18 15651.0 19 14447.0 20 14137.0 21 14827.0 22 17764.0 23 23647.0 24 32997.0 25 43494.0 26 51473.0 27 57708.0 28 67365.0 29 77970.0 30 90971.0 31 107042.0 32 130879.0 33 161429.0 34 207699.0 35 283579.0 36 451113.0 37 700375.0 38 272790.0 39 908.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.85772751637569 19.74623101697218 13.614894071307951 24.781147395344185 2 15.336800502005724 20.53597055584709 39.12777401618616 24.999454925961025 3 19.083747335572518 25.123245626339674 29.272201672165142 26.520805365922666 4 12.708297889486833 17.180132343591904 36.54259081832753 33.56897894859374 5 13.151403210605334 38.823677624726436 33.758195856299245 14.26672330836899 6 31.68533933580631 37.385808413311096 17.009483919239997 13.919368331642602 7 28.24904958058584 33.12670527835694 20.449730886109045 18.17451425494818 8 26.263152019950898 36.37938104700435 18.892947168678333 18.46451976436642 9 26.30990209851153 16.414190477981194 19.80136172097464 37.474545702532644 10-11 24.85114534876342 27.391973338529453 26.79283682018364 20.964044492523485 12-13 25.587733850377546 24.470693299696805 27.807532759498628 22.13404009042702 14-15 22.556471767026594 25.73608572850154 25.916243805573735 25.79119869889813 16-17 22.023937056122232 29.197513933620588 26.543657334088422 22.234891676168758 18-19 21.968148297263657 27.318349744965225 29.115792398514724 21.597709559256394 20-21 22.707724929379374 26.46879559298641 29.161229336154175 21.662250141480037 22-23 22.36389935834122 26.419345816681457 28.97293702538607 22.243817799591252 24-25 22.14151922655647 26.612713564569724 29.207706261456813 22.03806094741699 26-27 22.076814899663525 26.899212266603318 29.053973794747606 21.969999038985552 28-29 21.792990020231862 26.707760444357454 29.372122491228904 22.127127044181783 30-31 21.984847010678006 26.52383465059962 29.408579172966487 22.08273916575589 32-33 21.850191299063756 26.59946402237508 29.20188639769492 22.348458280866243 34-35 22.301862398250357 26.5600368408769 29.02330788336269 22.11479287751005 36-37 22.275995055240553 26.46638620099337 28.899116521191015 22.35850222257506 38-39 22.183391998987652 26.642946721000794 29.058167041024575 22.115494238986983 40-41 22.201018884384425 26.512392984533783 29.150452654287985 22.13613547679381 42-43 22.13091476600597 26.547612634264468 28.83883667731326 22.482635922416303 44-45 22.24036642704975 26.763745043463523 28.504568996957584 22.491319532529143 46-47 22.387385511980085 26.90367927044026 28.013293645197678 22.695641572381973 48-49 22.515529595506024 26.945845968438363 27.85767761981855 22.680946816237064 50-51 22.578786085806826 27.106368024306036 27.699633573911598 22.615212315975537 52-53 22.729439896449062 27.165228898578963 27.465665698139887 22.639665506832092 54-55 22.41296104071299 27.07586582328198 27.67432115670325 22.836851979301777 56-57 22.485487897490735 27.434965828902612 27.57137563793336 22.50817063567329 58-59 22.545536646814153 26.909259181606537 27.773500798582234 22.77170337299707 60-61 22.500377541390773 26.953694623034618 27.98505413615247 22.56087369942214 62-63 22.504108187544656 27.005490914235132 27.93081054107501 22.5595903571452 64-65 22.311784793556747 26.905610507472378 27.852946601301376 22.9296580976695 66-67 22.24894691320368 27.293049119776423 27.683334397811038 22.774669569208857 68-69 22.27026822142903 26.949045225665724 27.90358825867003 22.877098294235214 70-71 22.27265890015776 26.953784158542316 27.849333229074208 22.924223712225718 72-73 22.4835777721701 26.954061933724322 27.66662821544602 22.89573207865956 74-75 22.355587285138856 27.207288624864802 27.537310448238728 22.899813641757618 76-77 22.446841388111626 27.06301644521691 27.604604475693158 22.885537690978314 78-79 22.481711827557767 27.111890143674504 27.580314478520158 22.826083550247574 80-81 22.210162921435504 27.44583611395308 27.552608927669663 22.79139203694175 82-83 22.368526885409366 27.428995572011555 27.336758870041457 22.86571867253762 84-85 22.34565526516027 27.06825250039209 27.50885693099898 23.077235303448656 86-87 22.327548064045104 26.88457242455286 27.812508627166864 22.975370884235172 88-89 22.388562614866593 26.922163201742006 27.670098065257058 23.019176118134343 90-91 22.29117782766564 27.289020021930234 27.698958343903495 22.720843806500636 92-93 22.349037528386447 26.99802009335871 27.78222250676447 22.870719871490376 94-95 22.20978768428577 26.923399498871042 27.72154958982938 23.145263227013807 96-97 22.38350471401334 26.974698218381132 27.557473116931646 23.084323950673884 98-99 22.220530978047005 26.911062393330372 27.459249347241926 23.4091572813807 100-101 22.00907207535102 26.99383381212653 27.735817775352352 23.261276337170095 102-103 22.316009134424764 26.71768506230859 27.701756226419487 23.26454957684716 104-105 22.17978880854197 26.613027661538545 27.833566972451873 23.373616557467614 106-107 22.050522121615725 26.836670509362374 27.735935920251002 23.3768714487709 108-109 22.1192036772094 26.860694847641774 27.378979491119992 23.641121984028835 110-111 21.86864525845159 26.920265140690535 27.684621120674414 23.526468480183464 112-113 21.992842636647186 26.726862019518972 27.7322386227515 23.548056721082347 114-115 21.766378535267805 26.82978112210827 27.52368410253349 23.88015624009043 116-117 21.69370644940528 26.736420242962588 27.704110092739803 23.86576321489233 118-119 21.671636971186363 26.823154655064247 27.499577683292912 24.005630690456478 120-121 21.590289572964533 26.920282106458966 27.56661315397403 23.92281516660247 122-123 21.618964975649867 26.79457917580372 27.522553813163174 24.06390203538324 124-125 21.507782138910038 26.741792484055637 27.525557690855578 24.224867686178747 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 282.0 1 240.0 2 271.5 3 430.5 4 397.5 5 355.0 6 762.5 7 1614.0 8 2536.5 9 2999.5 10 2639.5 11 2136.5 12 2185.0 13 2415.0 14 2682.0 15 3002.5 16 3387.0 17 3709.5 18 4031.5 19 4386.5 20 4790.5 21 5600.5 22 6647.0 23 8084.0 24 9911.5 25 12479.0 26 16412.5 27 21305.5 28 25634.0 29 31466.0 30 39122.5 31 44163.0 32 49341.0 33 56977.5 34 65782.5 35 73602.5 36 82458.0 37 91742.0 38 97218.0 39 99430.5 40 100794.0 41 106170.5 42 113301.0 43 118769.0 44 123768.0 45 127431.0 46 127772.0 47 127873.0 48 133362.5 49 133302.5 50 127224.5 51 121858.0 52 115668.0 53 111832.0 54 109036.5 55 102227.5 56 90724.0 57 80796.0 58 74306.0 59 66724.5 60 56440.5 61 45784.5 62 36143.5 63 27675.0 64 21458.5 65 16792.5 66 12238.0 67 9663.5 68 7521.0 69 5232.0 70 3806.0 71 2913.0 72 2405.5 73 2054.5 74 1626.5 75 1262.5 76 848.5 77 480.5 78 305.5 79 183.5 80 113.0 81 83.0 82 55.5 83 39.5 84 24.5 85 14.0 86 11.5 87 7.0 88 5.0 89 3.0 90 1.0 91 1.0 92 0.5 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.057034118400561565 2 0.07332958080072202 3 0.0 4 0.007640363323152151 5 0.027308329846422726 6 2.0891618461744166E-4 7 0.0 8 0.0 9 3.879872000038202E-4 10-11 0.058899441477503006 12-13 0.008953550769318927 14-15 0.007013614769299826 16-17 0.05376607236976015 18-19 1.343032615397839E-4 20-21 0.009848905846250821 22-23 0.012027603200118425 24-25 0.0 26-27 0.0 28-29 0.05075171027742279 30-31 0.0 32-33 0.003372504123110129 34-35 0.0 36-37 2.387613538485047E-4 38-39 0.0 40-41 1.4922584615531544E-5 42-43 0.0030591298461839665 44-45 0.0 46-47 7.461292307765772E-5 48-49 2.238387692329732E-4 50-51 5.521356307746671E-4 52-53 1.343032615397839E-4 54-55 0.0 56-57 0.001805632738479317 58-59 1.4922584615531544E-5 60-61 0.0 62-63 0.0 64-65 5.969033846212618E-4 66-67 0.0 68-69 5.22290461543604E-4 70-71 0.0 72-73 0.001805632738479317 74-75 0.002686065230795678 76-77 4.924452923125409E-4 78-79 9.401228307784874E-4 80-81 0.001611639138477407 82-83 2.238387692329732E-4 84-85 4.476775384659464E-5 86-87 5.521356307746671E-4 88-89 0.0 90-91 0.0 92-93 0.0064316339692940955 94-95 0.005700427323133051 96-97 0.005670582153901987 98-99 0.0010595035077027397 100-101 4.3275495385041487E-4 102-103 1.343032615397839E-4 104-105 5.073678769280725E-4 106-107 0.0033874267077256604 108-109 0.0044916979692749956 110-111 8.20742153854235E-4 112-113 0.0027009878154112097 114-115 4.0290978461935176E-4 116-117 0.002059316676943353 118-119 0.0017907101538637857 120-121 0.009863828430866351 122-123 8.505873230852981E-4 124-125 0.002059316676943353 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3350626.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.47759426332836 #Duplication Level Percentage of deduplicated Percentage of total 1 75.04338423319071 36.37922733003672 2 12.15479037394963 11.784699922082789 3 4.813977465853257 7.001101391473197 4 2.465097670242857 4.78008018710037 5 1.424097959513539 3.4518421536265587 6 0.8510862970478674 2.475516971481904 7 0.5930319402980959 2.012413325087582 8 0.43469563512020865 1.6858398901917846 9 0.32568676761021914 1.4209659879428804 >10 1.672022688065189 14.765956419457238 >50 0.13759624945224 4.6381629117995695 >100 0.07913632931338432 7.026491816235761 >500 0.004403950740601308 1.3313239141718722 >1k 8.68384651888077E-4 0.7503524995161672 >5k 1.2405495026972528E-4 0.49602527979557165 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8926 0.2663979805564692 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7570 0.22592793107914758 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4668 0.13931724997060252 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 2.9845169231063092E-5 6 0.0 0.0 0.0 0.0 2.9845169231063092E-5 7 2.9845169231063092E-5 0.0 0.0 0.0 2.9845169231063092E-5 8 2.9845169231063092E-5 0.0 0.0 0.0 2.9845169231063092E-5 9 2.9845169231063092E-5 0.0 0.0 0.0 2.9845169231063092E-5 10-11 5.9690338462126184E-5 0.0 0.0 1.4922584615531546E-5 2.9845169231063092E-5 12-13 5.9690338462126184E-5 0.0 0.0 2.9845169231063092E-5 2.9845169231063092E-5 14-15 8.953550769318928E-5 0.0 0.0 4.476775384659464E-5 2.9845169231063092E-5 16-17 8.953550769318928E-5 0.0 0.0 7.461292307765774E-5 5.9690338462126184E-5 18-19 1.4922584615531545E-4 0.0 0.0 8.953550769318928E-5 5.9690338462126184E-5 20-21 2.0891618461744163E-4 0.0 0.0 8.953550769318928E-5 5.9690338462126184E-5 22-23 2.0891618461744163E-4 0.0 0.0 1.64148430770847E-4 5.9690338462126184E-5 24-25 2.6860652307956784E-4 0.0 0.0 3.28296861541694E-4 5.9690338462126184E-5 26-27 2.6860652307956784E-4 0.0 0.0 6.416711384678564E-4 5.9690338462126184E-5 28-29 4.4767753846594637E-4 0.0 0.0 9.401228307784874E-4 5.9690338462126184E-5 30-31 4.775227076970095E-4 0.0 0.0 0.0024622264615627052 5.9690338462126184E-5 32-33 4.775227076970095E-4 0.0 0.0 0.006058569353905807 5.9690338462126184E-5 34-35 5.22290461543604E-4 0.0 0.0 0.010192125292408045 5.9690338462126184E-5 36-37 6.56593723083388E-4 0.0 0.0 0.017623572430942754 5.9690338462126184E-5 38-39 6.56593723083388E-4 0.0 0.0 0.030934517907996895 5.9690338462126184E-5 40-41 6.56593723083388E-4 0.0 0.0 0.04708075446200202 5.9690338462126184E-5 42-43 7.013614769299827E-4 0.0 0.0 0.06168996480060741 5.9690338462126184E-5 44-45 7.461292307765773E-4 0.0 0.0 0.07983582769309377 5.9690338462126184E-5 46-47 7.610518153921089E-4 0.0 0.0 0.10211524652408235 5.9690338462126184E-5 48-49 8.356647384697665E-4 0.0 0.0 0.12836407286280235 5.9690338462126184E-5 50-51 8.356647384697665E-4 0.0 0.0 0.15646329969384826 5.9690338462126184E-5 52-53 8.505873230852981E-4 0.0 0.0 0.18481621046335817 5.9690338462126184E-5 54-55 8.655099077008296E-4 0.0 0.0 0.21482552812519212 5.9690338462126184E-5 56-57 9.252002461629558E-4 0.0 0.0 0.25078895704862314 5.9690338462126184E-5 58-59 9.252002461629558E-4 0.0 0.0 0.29209467126441446 5.9690338462126184E-5 60-61 9.252002461629558E-4 0.0 0.0 0.339638025849498 5.9690338462126184E-5 62-63 9.252002461629558E-4 0.0 0.0 0.3884348775422861 5.9690338462126184E-5 64-65 9.55045415394019E-4 0.0 0.0 0.4387090651120119 5.9690338462126184E-5 66-67 0.0010445809230872083 0.0 0.0 0.49733990006643536 5.9690338462126184E-5 68-69 0.0010445809230872083 0.0 0.0 0.5672074412363541 5.9690338462126184E-5 70-71 0.001119193846164866 0.0 0.0 0.6458643847448209 5.9690338462126184E-5 72-73 0.0012236519384735867 0.0 0.0 0.7284012002533258 5.9690338462126184E-5 74-75 0.001283342276935713 0.0 0.0 0.813892687515706 5.9690338462126184E-5 76-77 0.001283342276935713 0.0 0.0 0.9071289961935471 5.9690338462126184E-5 78-79 0.001283342276935713 0.0 0.0 1.0170785996407836 5.9690338462126184E-5 80-81 0.0016862520615550645 0.0 0.0 1.1448457691189646 5.9690338462126184E-5 82-83 0.0017011746461705962 0.0 0.0 1.2855508194588117 5.9690338462126184E-5 84-85 0.0018504004923259117 0.0 0.0 1.4264050956448138 5.9690338462126184E-5 86-87 0.0020145489230967584 0.0 0.0 1.5769888970001427 5.9690338462126184E-5 88-89 0.0020443940923278218 0.0 0.0 1.7526724856787954 5.9690338462126184E-5 90-91 0.002283155446176326 0.0 0.0 1.955545023526947 5.9690338462126184E-5 92-93 0.002342845784638453 0.0 0.0 2.1744294946675637 5.9690338462126184E-5 94-95 0.0023577683692539843 0.0 0.0 2.3951046759620445 5.9690338462126184E-5 96-97 0.0023577683692539843 0.0 0.0 2.620793845687343 5.9690338462126184E-5 98-99 0.0025517619692558943 0.0 0.0 2.869583176397485 5.9690338462126184E-5 100-101 0.002686065230795678 0.0 0.0 3.1668559845234894 5.9690338462126184E-5 102-103 0.0028054459077199306 0.0 0.0 3.4877810892651104 5.9690338462126184E-5 104-105 0.003193433107723751 0.0 0.0 3.8067811805913285 5.9690338462126184E-5 106-107 0.003357581538494598 0.0 0.0 4.1292134663791185 5.9690338462126184E-5 108-109 0.0034620396308033185 0.0 0.0 4.482893644351831 5.9690338462126184E-5 110-111 0.003566497723112039 0.0 0.0 4.885296061094255 5.9690338462126184E-5 112-113 0.003611265476958634 0.0 0.0 5.32900419205247 5.9690338462126184E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9520 0.0 43.686947 1 GTATCAA 15035 0.0 42.444286 1 ATCAACG 17975 0.0 35.18587 3 TCAACGC 18235 0.0 34.686768 4 TATCAAC 18330 0.0 34.372395 2 CAACGCA 18555 0.0 33.64018 5 AACGCAG 18860 0.0 33.277187 6 GTACATG 16015 0.0 31.773432 1 TACATGG 16650 0.0 29.828978 2 ACATGGG 17015 0.0 28.464031 3 ACGCAGA 21985 0.0 28.059566 7 CGCAGAG 21970 0.0 27.970312 8 GAGTACT 12065 0.0 25.02311 12-13 GCAGAGT 23810 0.0 24.73418 9 AGTACTT 12685 0.0 23.377579 12-13 GTACTTT 12945 0.0 23.29904 14-15 CATGGGA 10270 0.0 23.117392 4 CATGGGG 7195 0.0 22.907902 4 GTAAGGT 1060 0.0 22.453833 4 TAAGGTG 1215 0.0 22.059425 5 >>END_MODULE