FastQCFastQC Report
Thu 26 May 2016
SRR1364594_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364594_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2699068
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38110.1411968872218114No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA91150.042.4750021
ATCAACG108700.034.7940333
GTACATG149150.034.7833671
TCAACGC109500.034.3228954
CAACGCA113200.033.5699775
GGTATCA55950.033.1083151
TATCAAC119800.032.6639862
AACGCAG114150.032.3788726
CGCATAG3500.032.301218
TACATGG154400.032.249252
ACATGGG156700.030.2175183
ACGCAGA134950.026.9016447
CGCAGAG130800.026.0208518
CGCAGAT8050.025.1313768
CATGGGA98750.024.5485574
ACGCATA4700.024.0590047
CATGGGG57400.023.0361444
GCAGAGT145650.022.1014129
GTATAGG7850.022.0041751
AGTACAT111700.021.2222062
Produced by FastQC (version 0.11.5)