##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364594_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2699068 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.028081915683487 31.0 27.0 31.0 10.0 34.0 2 26.770638235124125 31.0 26.0 31.0 10.0 34.0 3 27.321527653249195 31.0 27.0 31.0 12.0 34.0 4 30.696272194698317 35.0 31.0 37.0 10.0 37.0 5 31.0552401792026 35.0 32.0 37.0 16.0 37.0 6 31.359548925777343 35.0 32.0 37.0 16.0 37.0 7 31.295214866761416 35.0 32.0 37.0 13.0 37.0 8 30.98700366200481 35.0 32.0 37.0 10.0 37.0 9 32.043042635457866 37.0 32.0 39.0 10.0 39.0 10-11 32.00080842720524 37.0 31.5 39.0 10.0 39.0 12-13 31.99335641043501 37.0 32.0 39.0 9.0 39.0 14-15 32.70460859081727 38.0 31.5 40.0 9.0 41.0 16-17 33.11979820441723 38.0 32.0 40.0 10.0 41.0 18-19 32.96266581649665 38.0 31.5 40.0 10.0 41.0 20-21 32.890265084095695 38.0 31.5 40.0 10.0 41.0 22-23 32.97427111877137 38.0 32.0 40.0 10.0 41.0 24-25 33.0810388993534 38.0 32.0 41.0 10.0 41.0 26-27 32.90315064311088 38.0 31.0 40.0 9.0 41.0 28-29 32.815829945744234 38.0 31.0 40.0 8.5 41.0 30-31 32.69028494280248 38.0 31.0 40.0 8.0 41.0 32-33 32.59465767442688 38.0 30.5 40.0 8.0 41.0 34-35 32.654234906271355 38.0 31.0 40.0 4.0 41.0 36-37 32.58769619735405 38.0 30.5 40.0 2.0 41.0 38-39 32.290713127642576 38.0 30.0 40.0 2.0 41.0 40-41 32.160966118675034 38.0 30.0 40.0 2.0 41.0 42-43 31.657973789471033 37.5 30.0 40.0 2.0 41.0 44-45 31.550856073281594 37.0 30.0 40.0 2.0 41.0 46-47 31.516251165217028 37.0 30.0 40.0 2.0 41.0 48-49 31.442983652134735 37.0 30.0 40.0 2.0 41.0 50-51 31.214976058402385 37.0 29.0 40.0 2.0 41.0 52-53 31.12056717355769 37.0 29.5 40.0 2.0 41.0 54-55 30.93909267939896 36.5 29.0 40.0 2.0 41.0 56-57 30.719522998309046 36.0 29.0 40.0 2.0 41.0 58-59 30.513238643857804 36.0 28.5 40.0 2.0 41.0 60-61 30.206485720256026 35.0 28.0 39.0 2.0 41.0 62-63 30.010690542068595 35.0 28.0 39.0 2.0 41.0 64-65 29.766025161277895 35.0 28.0 39.0 2.0 40.0 66-67 29.48535364799998 35.0 28.0 38.5 2.0 40.0 68-69 29.224878550670084 34.0 28.0 38.0 2.0 40.0 70-71 28.767560506070986 34.0 26.5 37.5 2.0 40.0 72-73 28.522537038711143 34.0 26.0 37.0 2.0 39.0 74-75 28.46712420731897 34.0 27.0 36.5 2.0 39.0 76-77 28.20587328663079 34.0 26.5 36.0 2.0 39.0 78-79 27.9155239882804 34.0 26.0 36.0 2.0 38.5 80-81 27.502412869923987 34.0 26.0 35.0 2.0 37.0 82-83 27.626979016460496 34.0 26.5 35.0 2.0 37.0 84-85 27.461563398921406 34.0 26.0 35.0 2.0 36.5 86-87 27.34249044484985 34.0 26.0 35.0 2.0 36.0 88-89 27.1859332554793 34.0 26.0 35.0 2.0 36.0 90-91 27.055666622700873 34.0 26.0 35.0 2.0 36.0 92-93 26.937651996911526 34.0 26.0 35.0 2.0 35.0 94-95 26.834960808693964 34.0 26.0 35.0 2.0 35.0 96-97 26.73091971006288 34.0 25.5 35.0 2.0 35.0 98-99 26.64307568390274 33.5 25.0 35.0 2.0 35.0 100-101 26.543599123845713 33.0 25.0 35.0 2.0 35.0 102-103 25.39031454561352 31.5 23.5 34.0 2.0 35.0 104-105 26.24480783737201 33.0 25.0 35.0 2.0 35.0 106-107 26.39963424411686 33.0 25.0 35.0 2.0 35.0 108-109 26.365978367347545 33.0 25.0 35.0 2.0 35.0 110-111 26.302471260449906 33.0 25.0 35.0 2.0 35.0 112-113 26.21439993360671 33.0 24.5 35.0 2.0 35.0 114-115 26.1100616953704 33.0 24.0 35.0 2.0 35.0 116-117 26.004672538817104 33.0 24.0 35.0 2.0 35.0 118-119 25.88684019817211 33.0 23.5 35.0 2.0 35.0 120-121 25.709848547720917 33.0 23.0 35.0 2.0 35.0 122-123 25.51327532318563 33.0 19.5 35.0 2.0 35.0 124-125 24.575598132392365 32.0 10.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.590828956014235 1101 2 1.794028709231931 1101 3 1.2646740735621549 1101 4 1.5023016004362297 1101 5 1.3882148707225426 1101 6 1.287173808530106 1101 7 1.3177821410449653 1101 8 1.548039447300411 1101 9 1.8540154430298372 1101 10-11 1.8759142985255863 1101 12-13 1.904511557149192 1101 14-15 2.2968556365446275 1101 16-17 1.8972079346710586 1101 18-19 1.888436419140028 1101 20-21 2.112628555386344 1101 22-23 1.7891036493358925 1101 24-25 1.8499157559862098 1101 26-27 2.0266235434170525 1101 28-29 2.0341583992637027 1101 30-31 2.156088119250583 1101 32-33 2.2568431759100562 1101 34-35 2.0446456400150836 1101 36-37 1.927816440259619 1101 38-39 1.8960130646498214 1101 40-41 1.9114938301576174 1101 42-43 2.0757446843329106 1101 44-45 1.9260517284618608 1101 46-47 1.7137439607489569 1101 48-49 1.4565967357721092 1101 50-51 1.5395994781964362 1101 52-53 1.4744116490569823 1101 54-55 1.4860544881044326 1101 56-57 1.4905343225267451 1101 58-59 1.5259530173423563 1101 60-61 1.556179606751087 1101 62-63 1.3807113940573217 1101 64-65 1.350989076761934 1101 66-67 1.4184698422332893 1101 68-69 1.2505850518133457 1101 70-71 1.1070056312081924 1101 72-73 1.1094704246049005 1101 74-75 1.1845192909389795 1101 76-77 1.2700170875683838 1101 78-79 1.2725587866959422 1101 80-81 1.3480404670233668 1101 82-83 1.3245210885762333 1101 84-85 1.3559507744165629 1101 86-87 1.3401363500735322 1101 88-89 1.251670169893135 1101 90-91 1.3249581084550748 1101 92-93 1.3866093959144195 1101 94-95 1.3345005452153345 1101 96-97 1.3486291021750958 1101 98-99 1.3849586577757265 1101 100-101 1.3411599935736085 1101 102-103 1.2198744366543046 1101 104-105 1.4382745555424847 1101 106-107 1.4147489855749669 1101 108-109 1.3457247280728488 1101 110-111 1.399863014773544 1101 112-113 1.2929193480399377 1101 114-115 1.330272475087014 1101 116-117 1.3522100667557133 1101 118-119 1.2915491933511234 1101 120-121 1.3374624593590134 1101 122-123 1.2783771671748063 1101 124-125 1.1951093306655665 1102 1 0.8570314893659159 1102 2 1.224546409812973 1102 3 0.8938428181297056 1102 4 0.8361524786712913 1102 5 0.6172864463236074 1102 6 0.5858281437235462 1102 7 0.8668758837083175 1102 8 0.8123952687715565 1102 9 1.3745704721664183 1102 10-11 1.371940777382406 1102 12-13 1.475113869958662 1102 14-15 1.6840995140377544 1102 16-17 1.3536853590289297 1102 18-19 1.1843908989271554 1102 20-21 1.5366968382132384 1102 22-23 1.6104965257222332 1102 24-25 1.2657571812480697 1102 26-27 1.585159319933446 1102 28-29 1.3384494748583364 1102 30-31 1.4144175527649168 1102 32-33 1.6077899307643477 1102 34-35 1.42515219750981 1102 36-37 1.3079756343715516 1102 38-39 1.5963381732047992 1102 40-41 1.6203489947978937 1102 42-43 1.8122849801465932 1102 44-45 1.449639557766698 1102 46-47 1.322841640322686 1102 48-49 1.222826539124064 1102 50-51 1.308296965814442 1102 52-53 1.0958683121681005 1102 54-55 1.127994776834182 1102 56-57 1.2643771263341357 1102 58-59 1.3106430122927044 1102 60-61 1.2268740420097544 1102 62-63 1.1075294959461957 1102 64-65 0.9730473411549312 1102 66-67 1.0500006703416993 1102 68-69 0.9174419711370874 1102 70-71 1.1110775124213248 1102 72-73 0.7242926100979474 1102 74-75 0.9872437206903655 1102 76-77 0.816043237304509 1102 78-79 0.8166962538269757 1102 80-81 1.0368899206359998 1102 82-83 1.0562268208243246 1102 84-85 0.9897760752720579 1102 86-87 1.0346449869841372 1102 88-89 0.9200441161975661 1102 90-91 1.0695494173492683 1102 92-93 1.0940189997816177 1102 94-95 1.0583084152120357 1102 96-97 0.8868271005570776 1102 98-99 1.0095393971939473 1102 100-101 1.058418800608262 1102 102-103 0.9154840919897147 1102 104-105 1.0642992258027348 1102 106-107 0.9515351188738634 1102 108-109 0.9732896204864296 1102 110-111 0.967055167031674 1102 112-113 0.901842342887786 1102 114-115 0.9839009680370943 1102 116-117 1.0548126591610512 1102 118-119 1.0243990118175539 1102 120-121 1.0737649106185891 1102 122-123 0.9702930539560342 1102 124-125 0.865665817336648 1103 1 0.9502445702795335 1103 2 1.2320956895991806 1103 3 0.8523692348529792 1103 4 0.7420236965157798 1103 5 0.7877579092101463 1103 6 0.9197245619965528 1103 7 0.9389461887420651 1103 8 1.0507897909811632 1103 9 1.5011792449421861 1103 10-11 1.604281510840579 1103 12-13 1.503211745883391 1103 14-15 1.7639960115180457 1103 16-17 1.3930314822735852 1103 18-19 1.407199855656522 1103 20-21 1.6762000058475692 1103 22-23 1.5351226639124178 1103 24-25 1.4309136853890294 1103 26-27 1.6994955938840306 1103 28-29 1.7035972949782234 1103 30-31 1.6562080089631905 1103 32-33 1.7923858788426728 1103 34-35 1.5637203626018064 1103 36-37 1.3974021567608474 1103 38-39 1.4334991076980543 1103 40-41 1.5993585333942093 1103 42-43 1.7862710815835925 1103 44-45 1.6004139340734902 1103 46-47 1.4872986365560905 1103 48-49 1.3288618774589693 1103 50-51 1.3137002976130532 1103 52-53 1.085159500220385 1103 54-55 1.1565477818026544 1103 56-57 1.2369163184536554 1103 58-59 1.1466365465760404 1103 60-61 1.2536136944950016 1103 62-63 1.0360255605142683 1103 64-65 0.9900791903027226 1103 66-67 1.0060284289276638 1103 68-69 0.9488712139774158 1103 70-71 0.9383301540561142 1103 72-73 0.8739525787441274 1103 74-75 0.8737597696434669 1103 76-77 0.8515784955249366 1103 78-79 0.7828515955729216 1103 80-81 0.8665803446696145 1103 82-83 0.8551631408602809 1103 84-85 0.9466524341446103 1103 86-87 0.8356664418342064 1103 88-89 0.8758040340472277 1103 90-91 0.896273697615662 1103 92-93 0.8578562334479116 1103 94-95 0.8622250025619316 1103 96-97 0.8559081690845751 1103 98-99 0.8481010993221361 1103 100-101 0.8550364307101326 1103 102-103 0.7173931251171872 1103 104-105 0.8332382279999564 1103 106-107 0.8105729355958431 1103 108-109 0.8655421911099488 1103 110-111 0.7648380075687093 1103 112-113 0.8581572747929869 1103 114-115 0.8584620042234938 1103 116-117 0.8511061713151484 1103 118-119 0.9093604770997068 1103 120-121 0.9371296284295063 1103 122-123 0.773785388086381 1103 124-125 0.6788518151159657 1104 1 1.1865337963130287 1104 2 1.5159834449236271 1104 3 1.0238680653659387 1104 4 1.213367553773221 1104 5 0.7702926959152947 1104 6 1.0006068532982155 1104 7 0.7313683399387614 1104 8 1.1050349634279542 1104 9 1.4206379854629887 1104 10-11 1.4924314250020245 1104 12-13 1.4281547525235183 1104 14-15 1.5502384512393306 1104 16-17 1.3006435584513554 1104 18-19 1.3639602868245717 1104 20-21 1.4545916036480975 1104 22-23 1.4338446577141042 1104 24-25 1.3778867764755134 1104 26-27 1.525575232514484 1104 28-29 1.580566339261381 1104 30-31 1.7033093347212969 1104 32-33 1.6378118392008858 1104 34-35 1.5887734408021075 1104 36-37 1.495524407727352 1104 38-39 1.5657708646062076 1104 40-41 1.5284262429277433 1104 42-43 1.7408904389646338 1104 44-45 1.5462269146313794 1104 46-47 1.314664347669222 1104 48-49 1.264132584508225 1104 50-51 1.2064589848740184 1104 52-53 1.1912345549161927 1104 54-55 1.201744775183645 1104 56-57 1.162332435336907 1104 58-59 1.1050774847016775 1104 60-61 1.1135950445500704 1104 62-63 0.9831986942110973 1104 64-65 0.8934349198391516 1104 66-67 0.8288814606676596 1104 68-69 0.6475853402022871 1104 70-71 0.7942707241070721 1104 72-73 0.7412422059805728 1104 74-75 0.7381736329968831 1104 76-77 0.6776657719766241 1104 78-79 0.7063304691420775 1104 80-81 0.8371414183957597 1104 82-83 0.8889953667405841 1104 84-85 0.8876547050613581 1104 86-87 0.7988482314800365 1104 88-89 0.8861728420795316 1104 90-91 0.8137530315653585 1104 92-93 0.7760700401572613 1104 94-95 0.7248819496974654 1104 96-97 0.7033816554438275 1104 98-99 0.7790173478801847 1104 100-101 0.850468672089626 1104 102-103 0.7292357842612809 1104 104-105 0.8742829788638531 1104 106-107 0.8126756339884054 1104 108-109 0.7558325270492681 1104 110-111 0.7967049847904306 1104 112-113 0.7858176070978189 1104 114-115 0.8527282048961347 1104 116-117 0.7515134534747041 1104 118-119 0.8574542166384553 1104 120-121 0.8486561811287068 1104 122-123 0.8628660732225413 1104 124-125 0.6952607115259326 1105 1 1.2040490029313204 1105 2 1.5043548722639741 1105 3 0.9850178425679204 1105 4 1.2134033796170165 1105 5 1.053333140872276 1105 6 0.8961533378146669 1105 7 0.8862340220725073 1105 8 1.1290351924228688 1105 9 1.304748763813329 1105 10-11 1.3295694084814684 1105 12-13 1.4163970676379591 1105 14-15 1.6707063912242717 1105 16-17 1.2281759856813181 1105 18-19 1.4610908364414215 1105 20-21 1.364915310722445 1105 22-23 1.4004165734962584 1105 24-25 1.1550295431892366 1105 26-27 1.5221559219973742 1105 28-29 1.544158779378975 1105 30-31 1.579870875532741 1105 32-33 1.5812035565562823 1105 34-35 1.3454223422074492 1105 36-37 1.0402103699998335 1105 38-39 1.2820540555560171 1105 40-41 1.2892374780091842 1105 42-43 1.411745461457457 1105 44-45 1.3032133172712612 1105 46-47 0.7762191929209479 1105 48-49 0.8127728848062574 1105 50-51 1.0042602826410132 1105 52-53 0.9408409850257868 1105 54-55 1.0423947503960314 1105 56-57 0.9933204467508254 1105 58-59 1.0646101063110507 1105 60-61 0.9835305894998854 1105 62-63 0.9586445565197934 1105 64-65 0.6687496910282036 1105 66-67 0.6036850176413999 1105 68-69 0.5234529973567064 1105 70-71 0.8865537405269279 1105 72-73 0.7790538424082882 1105 74-75 0.8804383915500615 1105 76-77 0.8139165090691485 1105 78-79 0.7800715235741222 1105 80-81 0.9326670972955 1105 82-83 0.865910572777917 1105 84-85 0.8387930651962954 1105 86-87 0.9582469418948989 1105 88-89 0.9390529978541693 1105 90-91 0.7413218292887755 1105 92-93 0.9370527430525684 1105 94-95 0.846446723307178 1105 96-97 0.8954755331944284 1105 98-99 1.0035569736840912 1105 100-101 0.9886047139535741 1105 102-103 0.7965761764961776 1105 104-105 0.851173978249971 1105 106-107 0.8587343251928701 1105 108-109 0.8317802900642199 1105 110-111 0.8811877104399883 1105 112-113 0.8261859500266695 1105 114-115 0.8040470558741788 1105 116-117 0.8064151974670999 1105 118-119 0.8870370743110918 1105 120-121 0.8096976638248243 1105 122-123 0.7482349014947509 1105 124-125 0.7230995707536074 1106 1 0.23090879623785554 1106 2 -1.763002428815028 1106 3 -0.2046816345401652 1106 4 0.32108701694426145 1106 5 0.44208669586550187 1106 6 0.4809172993274373 1106 7 0.5941745333197268 1106 8 0.5203397583031482 1106 9 0.9328267254679261 1106 10-11 1.043591838995809 1106 12-13 1.055430834941717 1106 14-15 1.1457060296599906 1106 16-17 0.840456621138685 1106 18-19 0.8942427928073826 1106 20-21 1.0873807335147063 1106 22-23 0.8075525809122936 1106 24-25 0.9129062208474537 1106 26-27 0.9568273230565936 1106 28-29 1.0900148679346913 1106 30-31 1.1648911394606642 1106 32-33 1.2267930089566903 1106 34-35 1.0773559086214348 1106 36-37 0.8337632358282292 1106 38-39 1.163483659692119 1106 40-41 1.1594439522023592 1106 42-43 1.3145124311849585 1106 44-45 1.1625202615446462 1106 46-47 0.9563852115711313 1106 48-49 0.8624269727685459 1106 50-51 0.9367274246130677 1106 52-53 0.8937173221137868 1106 54-55 0.8337566533717755 1106 56-57 0.8644231338031894 1106 58-59 0.8665845621451602 1106 60-61 0.9186931348003888 1106 62-63 0.871058527549863 1106 64-65 0.8788319457336726 1106 66-67 0.796779874844507 1106 68-69 0.809339475972628 1106 70-71 0.7521572682745372 1106 72-73 0.5831893445674154 1106 74-75 0.6044521258605045 1106 76-77 0.5330435272975897 1106 78-79 0.6217014156553518 1106 80-81 0.7808445101188575 1106 82-83 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70-71 -4.820035406759892 2216 72-73 -3.0318957699948115 2216 74-75 -2.6006627989261304 2216 76-77 -2.405819786611911 2216 78-79 -2.5822589027824634 2216 80-81 -2.5872041651629694 2216 82-83 -2.529175252249768 2216 84-85 -2.473938800805918 2216 86-87 -2.612610616244634 2216 88-89 -2.5930898592886855 2216 90-91 -2.490121969160839 2216 92-93 -2.4526205681323674 2216 94-95 -2.6441332877670156 2216 96-97 -2.63769860855529 2216 98-99 -2.7607495047212254 2216 100-101 -2.7623711996676867 2216 102-103 -2.512106686183781 2216 104-105 -2.6021621236326453 2216 106-107 -2.548155901828167 2216 108-109 -2.6169323345205022 2216 110-111 -2.434710683801427 2216 112-113 -2.737089243771777 2216 114-115 -2.7844773073594844 2216 116-117 -2.5212806773944756 2216 118-119 -2.5831075823887986 2216 120-121 -2.814627502288136 2216 122-123 -2.6363615824476625 2216 124-125 -2.334513939766879 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 187347.0 3 25701.0 4 16010.0 5 12799.0 6 13099.0 7 15047.0 8 16024.0 9 17089.0 10 18599.0 11 20668.0 12 22573.0 13 23428.0 14 23194.0 15 19342.0 16 15017.0 17 13165.0 18 12084.0 19 11333.0 20 10962.0 21 12039.0 22 15103.0 23 21330.0 24 32346.0 25 42864.0 26 47920.0 27 50232.0 28 58471.0 29 66857.0 30 77937.0 31 91092.0 32 110763.0 33 141867.0 34 187847.0 35 262846.0 36 395570.0 37 465104.0 38 125231.0 39 168.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.97925720017492 19.5708294075851 13.763097892383641 22.686815499856333 2 13.53759241570989 20.77944119040793 41.99446359894293 23.68850279493925 3 19.40474306126832 26.575143552918952 27.880031597619332 26.1400817881934 4 11.788989547556113 17.408046542817498 37.306921423701986 33.4960424859244 5 13.559125511176386 39.72410968719201 32.87512653984686 13.841638261784745 6 31.354801291552924 37.31226737640688 17.702748263027665 13.630183069012533 7 28.752081473079617 33.419147881584834 20.22332326553192 17.605447379803632 8 25.548713852147937 36.67351414435978 19.541666914136044 18.236105089356244 9 26.075294262634944 17.79911705433349 19.930161692719615 36.195426990311944 10-11 24.12213400608444 28.46191659864955 27.02047933516361 20.395470060102397 12-13 25.069230722131397 25.66658632655897 27.485983619895176 21.778199331414445 14-15 22.234769137582152 26.792949050079944 25.359105707834072 25.613176104503825 16-17 22.223494109348568 30.189092304250075 25.50883421464598 22.078579371755374 18-19 22.226601709224703 28.639432566941665 27.669840124600114 21.464125599233515 20-21 23.17287403663465 27.51757220334341 27.580236297633853 21.72931746238809 22-23 22.963974559971284 27.463734855612227 27.272027048822668 22.30026353559382 24-25 22.689830762318405 27.57977922324486 27.42628108587823 22.304108928558502 26-27 22.41698264870095 27.734319223275588 27.51818695478361 22.330511173239852 28-29 22.282487989058648 27.723050598195254 27.707371850357575 22.28708956238852 30-31 22.3610564103915 27.506440565274502 27.708372020629845 22.424131003704147 32-33 22.247251273799947 27.551789444186177 27.54583220823231 22.65512707378157 34-35 22.79022397591334 27.387949433590542 27.493152349442123 22.328674241053996 36-37 22.80512663351709 27.216596704285823 27.287665967413883 22.69061069478321 38-39 22.562173886095664 27.46644081711122 27.531983704093538 22.439401592699575 40-41 22.6368097797409 27.25703354120455 27.68637461995092 22.41978205910363 42-43 22.323446676574495 27.34744796625147 27.569686945029375 22.759418412144655 44-45 22.587757464881243 27.506368782667263 27.271730397786836 22.63414335466466 46-47 22.71578924715863 27.732845647185933 26.847356165237446 22.704008940417992 48-49 22.68262121750694 27.73736101701078 26.804932926770448 22.77508483871183 50-51 22.58092884495503 27.746905334401994 26.87297770653368 22.7991881141093 52-53 23.031538254698784 27.6307348459923 26.759333126607853 22.578393772701066 54-55 22.715005506122605 27.542673609854802 26.884814883879727 22.857506000142873 56-57 22.610585939790305 27.884314946440934 26.819219513860492 22.68587959990827 58-59 22.85909428260165 27.460618583320766 26.724734558835937 22.95555257524164 60-61 22.906132971395348 27.52438573258863 26.94921675130727 22.620264544708753 62-63 22.730346164098727 27.59828054152591 26.96785077151359 22.703522522861775 64-65 22.645414294562432 27.445450800126657 26.877332956900617 23.03180194841029 66-67 22.521333336053576 27.879348900407464 26.66518166233561 22.934136101203354 68-69 22.6506782987972 27.50963877790664 27.013617538560215 22.826065384735944 70-71 22.50556252545704 27.644137851214406 27.06436652498518 22.78593309834337 72-73 22.77816668629238 27.63268451030443 26.655194123041177 22.933954680362014 74-75 22.610507646820587 27.800947526299158 26.793746504448347 22.794798322431912 76-77 22.875947314134088 27.603916365741387 26.865800093370456 22.65433622675407 78-79 22.817141342492043 27.691645924350507 26.7303673258763 22.760845407281153 80-81 22.47558151355659 27.7911426360167 27.019779660400683 22.71349619002602 82-83 22.78840204918527 27.897229073349365 26.659202828714196 22.65516604875117 84-85 22.64093687351703 27.758713817014662 26.696688385979545 22.90366092348876 86-87 22.66080300804722 27.60101517512031 26.972978077701825 22.765203739130644 88-89 22.84382094097933 27.625284691553535 26.791766449401898 22.739127918065233 90-91 22.62821019389254 27.833064166962547 26.96954283269872 22.569182806446193 92-93 22.64860762696568 27.836219080714912 26.851632509313745 22.663540783005658 94-95 22.612756986551524 27.546486866301773 26.92975439616011 22.911001750986586 96-97 22.865996476203428 27.60900365829971 26.626351594158965 22.8986482713379 98-99 22.494598290338253 27.60427150151296 26.611585027742723 23.289545180406066 100-101 22.36167893108346 27.633424452441428 26.963571028204143 23.041325588270972 102-103 22.6610133765587 27.654122107206923 26.728680668894732 22.95618384733964 104-105 22.561559177677704 27.485254731469183 26.992336169865496 22.960849920987616 106-107 22.378444351083374 27.749920936776316 26.988984939499243 22.882649772641063 108-109 22.44380552503488 27.786953046348906 26.5074529295737 23.261788499042517 110-111 22.326378804283923 27.67601368130831 26.883748568681586 23.113858945726186 112-113 22.541365667400097 27.730560570278286 26.93216725962479 22.795906502696823 114-115 22.425275722579155 27.9073308066413 26.677784457765686 22.98960901301386 116-117 22.168297543068398 27.84608408850035 26.890350027214758 23.095268341216496 118-119 22.284611729000652 27.93129988824773 26.671134038013573 23.11295434473805 120-121 22.215415729512124 27.980550076267118 26.82273970931538 22.981294484905376 122-123 22.319278951809576 28.01700620346244 26.69740270578526 22.966312138942722 124-125 22.275451655855115 27.779755877738587 26.786298443358962 23.15849402304734 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5042.0 1 3775.5 2 2020.5 3 1397.0 4 1081.0 5 856.0 6 845.5 7 991.5 8 1236.5 9 1336.5 10 1267.0 11 1294.5 12 1382.0 13 1479.5 14 1594.5 15 1753.5 16 1957.5 17 2127.0 18 2391.0 19 2721.0 20 3114.0 21 3912.0 22 5006.5 23 6308.0 24 7940.0 25 10293.5 26 13800.0 27 17811.5 28 20991.0 29 24666.0 30 30224.0 31 36119.5 32 42846.5 33 49439.5 34 56447.5 35 62040.5 36 67011.0 37 73785.5 38 78256.5 39 80085.5 40 80769.0 41 83189.0 42 86258.5 43 89988.0 44 94108.0 45 95840.0 46 94986.5 47 95303.5 48 100781.5 49 102919.5 50 99875.0 51 95905.5 52 91689.0 53 89919.5 54 88200.5 55 82715.0 56 76979.0 57 72578.0 58 67955.0 59 61141.5 60 51311.0 61 41007.0 62 31885.0 63 24269.5 64 18130.0 65 13885.5 66 10417.0 67 7758.0 68 5814.5 69 4234.5 70 3183.5 71 2545.0 72 2113.5 73 1851.0 74 1475.0 75 1103.5 76 793.0 77 545.0 78 410.5 79 298.0 80 210.0 81 153.0 82 110.0 83 82.5 84 62.0 85 47.5 86 38.0 87 28.5 88 17.5 89 11.0 90 10.0 91 6.5 92 4.5 93 5.0 94 5.5 95 3.0 96 5.0 97 4.5 98 1.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19688277583225022 2 0.23504409670301007 3 0.2862469563567868 4 0.2724273712259194 5 0.3044013711399639 6 0.21770477809377162 7 0.20981316513700282 8 0.19013970748421308 9 0.19010265765812495 10-11 0.13589876209121074 12-13 0.1583694816136533 14-15 0.17546797635331898 16-17 0.14949604826554944 18-19 0.17370810961413347 20-21 0.1682988350052685 22-23 0.1898618337885522 24-25 0.1814885730926379 26-27 0.23239503413770976 28-29 0.16070362065720464 30-31 0.14251215604793951 32-33 0.18026592883173007 34-35 0.14080786404788617 36-37 0.14115983739572327 38-39 0.17224464148365287 40-41 0.19164022544078177 42-43 0.2564218463558532 44-45 0.1586844051354023 46-47 0.1441979231349488 48-49 0.14649501235241202 50-51 0.15220068556998195 52-53 0.17315236222281175 54-55 0.160814770135469 56-57 0.15879555461366665 58-59 0.13347199848243912 60-61 0.1462356635697952 62-63 0.13651008422166466 64-65 0.1329533009172055 66-67 0.1196153635254836 68-69 0.12287574822123785 70-71 0.12411691739518975 72-73 0.10283179230756691 74-75 0.10314671582931591 76-77 0.12428364161258627 78-79 0.1293594677866582 80-81 0.13982604365655107 82-83 0.142660355352292 84-85 0.12834059756923502 86-87 0.1542569509178724 88-89 0.14990359635251874 90-91 0.1374178049608235 92-93 0.1379550274391012 94-95 0.15936982691803245 96-97 0.14684698570024912 98-99 0.1428826543088207 100-101 0.1233388710473393 102-103 0.16131494278765857 104-105 0.12181982817772653 106-107 0.12746992665616427 108-109 0.1377697783086606 110-111 0.1158548061775398 112-113 0.10524003100329446 114-115 0.10333196495975648 116-117 0.10820401709034377 118-119 0.09182799395939635 120-121 0.08508492561135918 122-123 0.1108901294817322 124-125 0.10662939948159883 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2699068.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.37719317859712 #Duplication Level Percentage of deduplicated Percentage of total 1 77.02067996372504 41.111477131706565 2 11.6919932468615 12.481715643611583 3 4.453841500960232 7.132006745508217 4 2.1478967836375293 4.585948061916313 5 1.2374798065084383 3.302659934330695 6 0.7593623219896567 2.4319577612033982 7 0.5044564746375464 1.8848529487845673 8 0.3462383429878412 1.4784984735599491 9 0.27865913568206163 1.3386638264654078 >10 1.387251065653095 13.545527354294881 >50 0.11152891600816207 4.08120360715839 >100 0.058283110634509736 5.500001656253182 >500 0.0017469980358437857 0.5509123526785742 >1k 5.823326786094384E-4 0.5745745025281939 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3811 0.1411968872218114 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 3.704982608811634E-5 0.0 18-19 0.0 3.704982608811634E-5 0.0 5.557473913217451E-5 0.0 20-21 0.0 3.704982608811634E-5 0.0 9.262456522029085E-5 0.0 22-23 0.0 3.704982608811634E-5 0.0 2.2229895652869805E-4 0.0 24-25 0.0 3.704982608811634E-5 0.0 3.890231739252216E-4 0.0 26-27 0.0 3.704982608811634E-5 0.0 6.668968695860942E-4 0.0 28-29 0.0 3.704982608811634E-5 0.0 0.0012411691739518975 0.0 30-31 0.0 3.704982608811634E-5 0.0 0.002926936260961191 0.0 32-33 0.0 3.704982608811634E-5 0.0 0.0063910950002000685 0.0 34-35 0.0 3.704982608811634E-5 0.0 0.010966748522082438 0.0 36-37 0.0 3.704982608811634E-5 0.0 0.018561962870146286 0.0 38-39 0.0 3.704982608811634E-5 0.0 0.03254827221841021 0.0 40-41 0.0 3.704982608811634E-5 0.0 0.05103613543638026 0.0 42-43 0.0 3.704982608811634E-5 0.0 0.06793085613256131 0.0 44-45 0.0 3.704982608811634E-5 0.0 0.08545542387224034 0.0 46-47 0.0 3.704982608811634E-5 0.0 0.10920436239472292 0.0 48-49 0.0 3.704982608811634E-5 0.0 0.13797355235214526 0.0 50-51 0.0 3.704982608811634E-5 0.0 0.1690583564400749 0.0 52-53 0.0 3.704982608811634E-5 0.0 0.20071743283237028 0.0 54-55 0.0 3.704982608811634E-5 0.0 0.23389555209427848 0.0 56-57 0.0 3.704982608811634E-5 0.0 0.27392788918248817 0.0 58-59 0.0 3.704982608811634E-5 0.0 0.31859145453171245 0.0 60-61 0.0 3.704982608811634E-5 0.0 0.37066498509855994 1.852491304405817E-5 62-63 0.0 3.704982608811634E-5 0.0 0.4240537844915356 3.704982608811634E-5 64-65 0.0 3.704982608811634E-5 0.0 0.47809095584105327 3.704982608811634E-5 66-67 0.0 3.704982608811634E-5 0.0 0.5419092812778337 3.704982608811634E-5 68-69 0.0 3.704982608811634E-5 0.0 0.6183060226715296 3.704982608811634E-5 70-71 0.0 3.704982608811634E-5 0.0 0.7073552796743172 3.704982608811634E-5 72-73 0.0 3.704982608811634E-5 0.0 0.7981458785032463 3.704982608811634E-5 74-75 0.0 3.704982608811634E-5 0.0 0.8868061123321087 3.704982608811634E-5 76-77 0.0 3.704982608811634E-5 0.0 0.9848955269003967 3.704982608811634E-5 78-79 0.0 3.704982608811634E-5 0.0 1.100935582208377 3.704982608811634E-5 80-81 0.0 3.704982608811634E-5 0.0 1.2351485772125788 3.704982608811634E-5 82-83 1.852491304405817E-5 3.704982608811634E-5 0.0 1.3822919615215326 3.704982608811634E-5 84-85 3.704982608811634E-5 3.704982608811634E-5 0.0 1.5284535254391516 3.704982608811634E-5 86-87 3.704982608811634E-5 3.704982608811634E-5 0.0 1.685915286313646 3.704982608811634E-5 88-89 3.704982608811634E-5 3.704982608811634E-5 0.0 1.869052576667205 3.704982608811634E-5 90-91 3.704982608811634E-5 3.704982608811634E-5 0.0 2.0833672956739138 3.704982608811634E-5 92-93 3.704982608811634E-5 3.704982608811634E-5 0.0 2.3143914862463637 3.704982608811634E-5 94-95 3.704982608811634E-5 3.704982608811634E-5 0.0 2.543581710427451 3.704982608811634E-5 96-97 3.704982608811634E-5 3.704982608811634E-5 0.0 2.773438831478125 3.704982608811634E-5 98-99 3.704982608811634E-5 3.704982608811634E-5 0.0 3.0292493557035245 3.704982608811634E-5 100-101 3.704982608811634E-5 3.704982608811634E-5 0.0 3.331853810278215 3.704982608811634E-5 102-103 3.704982608811634E-5 3.704982608811634E-5 0.0 3.6643945243321028 3.704982608811634E-5 104-105 3.704982608811634E-5 3.704982608811634E-5 0.0 3.99400830212503 3.704982608811634E-5 106-107 3.704982608811634E-5 3.704982608811634E-5 0.0 4.329716776309452 3.704982608811634E-5 108-109 3.704982608811634E-5 7.409965217623268E-5 0.0 4.683672289842272 3.704982608811634E-5 110-111 3.704982608811634E-5 7.409965217623268E-5 0.0 5.089608709376718 3.704982608811634E-5 112-113 3.704982608811634E-5 7.409965217623268E-5 0.0 5.538930475260349 3.704982608811634E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 9115 0.0 42.475002 1 ATCAACG 10870 0.0 34.794033 3 GTACATG 14915 0.0 34.783367 1 TCAACGC 10950 0.0 34.322895 4 CAACGCA 11320 0.0 33.569977 5 GGTATCA 5595 0.0 33.108315 1 TATCAAC 11980 0.0 32.663986 2 AACGCAG 11415 0.0 32.378872 6 CGCATAG 350 0.0 32.30121 8 TACATGG 15440 0.0 32.24925 2 ACATGGG 15670 0.0 30.217518 3 ACGCAGA 13495 0.0 26.901644 7 CGCAGAG 13080 0.0 26.020851 8 CGCAGAT 805 0.0 25.131376 8 CATGGGA 9875 0.0 24.548557 4 ACGCATA 470 0.0 24.059004 7 CATGGGG 5740 0.0 23.036144 4 GCAGAGT 14565 0.0 22.101412 9 GTATAGG 785 0.0 22.004175 1 AGTACAT 11170 0.0 21.222206 2 >>END_MODULE