FastQCFastQC Report
Thu 26 May 2016
SRR1364594_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364594_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2699068
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99910.3701648124463704No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83140.3080322540965993No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54860.20325534591940625No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79600.048.5117761
GTATCAA130950.045.689191
ATCAACG166200.035.765753
TCAACGC168450.035.2553144
TATCAAC170200.034.8830642
CAACGCA168850.034.852315
AACGCAG173700.034.006256
GTACATG142600.031.6345421
TACATGG144150.030.9005342
ACGCAGA202650.028.8249957
ACATGGG150300.028.741573
CGCAGAG202600.028.5969858
GCAGAGT217200.025.9633759
GAGTACT119650.025.0609812-13
AGTACTT126600.024.24969312-13
CATGGGA97600.024.2050384
GTACTTT126250.023.60934614-15
AGAGTAC209600.022.45558410-11
CATGGGG53200.022.3708974
AGTACAT107550.021.2617932
Produced by FastQC (version 0.11.5)