##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364594_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2699068 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.73170479587769 31.0 30.0 33.0 26.0 34.0 2 29.855594227340696 31.0 30.0 34.0 26.0 34.0 3 29.821385752415278 31.0 30.0 34.0 26.0 34.0 4 33.51487217069003 35.0 33.0 37.0 28.0 37.0 5 32.928858776436904 35.0 33.0 37.0 26.0 37.0 6 32.92122762375753 35.0 33.0 37.0 26.0 37.0 7 32.73311528275686 35.0 33.0 37.0 25.0 37.0 8 32.6632485732112 35.0 33.0 37.0 25.0 37.0 9 33.96473782802064 38.0 34.0 39.0 25.0 39.0 10-11 33.75113631816612 38.0 33.0 39.0 23.0 39.0 12-13 33.619838773976795 37.0 33.0 39.0 22.0 39.0 14-15 34.60066252499011 38.5 33.0 41.0 22.0 41.0 16-17 34.52646635801692 38.0 33.0 41.0 20.5 41.0 18-19 34.57939462807161 39.0 33.5 41.0 22.0 41.0 20-21 34.604899543101546 39.0 34.0 41.0 22.0 41.0 22-23 34.5872145496149 39.0 34.0 41.0 22.0 41.0 24-25 34.57215805604008 39.0 34.0 41.0 22.0 41.0 26-27 34.48119962149897 39.0 33.5 41.0 22.0 41.0 28-29 34.237200952328735 39.0 33.0 41.0 19.0 41.0 30-31 34.16499547251125 38.0 33.0 41.0 19.5 41.0 32-33 33.9757227309575 38.0 33.0 41.0 18.5 41.0 34-35 33.83058726197339 38.0 33.0 41.0 18.0 41.0 36-37 33.646269934658925 38.0 33.0 40.0 16.0 41.0 38-39 33.49979604070738 38.0 33.0 40.0 15.5 41.0 40-41 33.3160944815025 38.0 32.0 40.0 14.0 41.0 42-43 33.118222104815445 38.0 32.0 40.0 9.0 41.0 44-45 32.8984964439577 38.0 32.0 40.0 8.5 41.0 46-47 32.60392531792456 38.0 31.0 40.0 4.0 41.0 48-49 32.318313025088656 38.0 31.0 40.0 2.0 41.0 50-51 32.11367553540704 38.0 31.0 40.0 2.0 41.0 52-53 31.94012192356769 37.5 30.0 40.0 2.0 41.0 54-55 31.71645101197895 37.0 30.0 40.0 2.0 41.0 56-57 31.49547955071899 37.0 30.0 40.0 2.0 41.0 58-59 31.30347419924211 36.5 30.0 40.0 2.0 41.0 60-61 31.07649140369935 36.0 29.0 40.0 2.0 41.0 62-63 30.80277562477122 36.0 29.0 39.5 2.0 41.0 64-65 30.52478170242469 35.0 29.0 39.0 2.0 41.0 66-67 30.251099453589163 35.0 29.0 39.0 2.0 41.0 68-69 29.930875768969138 35.0 28.5 38.5 2.0 40.0 70-71 29.672423777392787 35.0 28.0 38.0 2.0 40.0 72-73 29.511314461139918 35.0 29.0 37.0 2.0 40.0 74-75 29.22106001034431 35.0 28.5 37.0 2.0 39.0 76-77 28.9294460161804 34.0 28.0 36.0 2.0 39.0 78-79 28.635560126680765 34.0 28.0 36.0 2.0 38.5 80-81 28.367655612974552 34.0 27.5 35.5 2.0 37.0 82-83 28.12053494020899 34.0 27.0 35.0 2.0 37.0 84-85 28.111622048796104 34.0 28.0 35.0 2.0 36.5 86-87 27.975361309904013 34.0 28.0 35.0 2.0 36.0 88-89 27.83661545392706 34.0 27.5 35.0 2.0 36.0 90-91 27.6932956116704 34.0 27.0 35.0 2.0 36.0 92-93 27.562438589913256 34.0 27.0 35.0 2.0 35.0 94-95 27.483234212698605 34.0 27.0 35.0 2.0 35.0 96-97 27.405044630220505 34.0 27.0 35.0 2.0 35.0 98-99 27.31740141411776 34.0 27.0 35.0 2.0 35.0 100-101 27.225722545708372 34.0 26.0 35.0 2.0 35.0 102-103 26.05326023649645 32.0 24.5 34.0 2.0 35.0 104-105 26.97583665917272 33.5 25.5 35.0 2.0 35.0 106-107 27.144578054350614 34.0 26.0 35.0 2.0 35.0 108-109 27.158543060048878 34.0 27.0 35.0 2.0 35.0 110-111 27.103058722492356 34.0 26.0 35.0 2.0 35.0 112-113 27.05694669419222 34.0 26.0 35.0 2.0 35.0 114-115 26.97627810785056 34.0 26.0 35.0 2.0 35.0 116-117 26.859423141617775 34.0 25.0 35.0 2.0 35.0 118-119 26.758593521912008 34.0 25.0 35.0 2.0 35.0 120-121 26.6270983169005 34.0 25.0 35.0 2.0 35.0 122-123 26.44644447638963 34.0 25.0 35.0 2.0 35.0 124-125 25.64365921866363 33.0 22.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7525560672790874 1101 2 0.7288805914490766 1101 3 0.6158199107195692 1101 4 0.5448593166573161 1101 5 0.885843883338211 1101 6 0.859464751348213 1101 7 0.9051741079339664 1101 8 0.9519719182565396 1101 9 0.9800748833657451 1101 10-11 1.0735133395667518 1101 12-13 1.0406631936946908 1101 14-15 1.1807378070234549 1101 16-17 1.2208433637355967 1101 18-19 1.2229415174766771 1101 20-21 1.2934401610873252 1101 22-23 1.230069899889692 1101 24-25 1.2360119237589586 1101 26-27 1.317042854253927 1101 28-29 1.3470026876155714 1101 30-31 1.3410244247568102 1101 32-33 1.3109406206851801 1101 34-35 1.332912040070518 1101 36-37 1.2536783605748383 1101 38-39 1.29064750732379 1101 40-41 1.3566143020145205 1101 42-43 1.325314628613917 1101 44-45 1.1648957379974618 1101 46-47 1.2591769630828296 1101 48-49 1.3662911638485582 1101 50-51 1.289298411479109 1101 52-53 1.2535015790452881 1101 54-55 1.2274790095803425 1101 56-57 1.1465387953890556 1101 58-59 1.069954130694061 1101 60-61 1.0722641247335005 1101 62-63 1.12302484811741 1101 64-65 1.040112467666642 1101 66-67 1.0639362223966806 1101 68-69 0.995560128084918 1101 70-71 1.072930884221531 1101 72-73 1.0733900869238369 1101 74-75 0.9451403184554685 1101 76-77 0.9490797599284484 1101 78-79 0.9831293044814728 1101 80-81 1.0458965356440224 1101 82-83 0.9770799700334223 1101 84-85 1.0185341589289933 1101 86-87 1.069919280124605 1101 88-89 0.9904630054162453 1101 90-91 1.0800461465533857 1101 92-93 1.0495228830637018 1101 94-95 0.9904222726712817 1101 96-97 1.0107906089788017 1101 98-99 1.057881069294222 1101 100-101 1.0768601922474694 1101 102-103 1.0599200536318456 1101 104-105 0.984334840829181 1101 106-107 0.9308502482925363 1101 108-109 0.9094738183560516 1101 110-111 0.954712394026263 1101 112-113 0.9080216235061087 1101 114-115 0.8788462459002133 1101 116-117 0.8591371288789489 1101 118-119 0.9623849064821464 1101 120-121 1.0028929699561715 1101 122-123 0.9799597712088364 1101 124-125 0.8167880955956939 1102 1 0.33063619030126645 1102 2 -0.7507636071404669 1102 3 -0.13384311670809623 1102 4 0.24148100245111692 1102 5 0.6401097733636334 1102 6 0.4918482841548055 1102 7 0.5703484682408799 1102 8 0.48506644579438074 1102 9 0.7489630729166947 1102 10-11 0.8399068569548191 1102 12-13 0.8047470987838068 1102 14-15 0.9267758051045334 1102 16-17 1.009241012998885 1102 18-19 0.9726394316977931 1102 20-21 0.8714778432125314 1102 22-23 0.8637029942605068 1102 24-25 0.9217295902368221 1102 26-27 0.8957015714543104 1102 28-29 1.0154114580237987 1102 30-31 0.939541406914401 1102 32-33 0.966601574965928 1102 34-35 0.8602330383782117 1102 36-37 0.9136487898641974 1102 38-39 0.7628391282870908 1102 40-41 0.7235014377523612 1102 42-43 0.586694227331165 1102 44-45 0.4558189654239371 1102 46-47 0.5047252922624494 1102 48-49 0.6245804759831728 1102 50-51 0.5071535538645549 1102 52-53 0.5696782372415434 1102 54-55 0.6748786269853682 1102 56-57 0.4106084699263093 1102 58-59 0.5709695463938651 1102 60-61 0.4660035282226147 1102 62-63 0.7071941427866903 1102 64-65 0.6255308537277209 1102 66-67 0.6088627955700829 1102 68-69 0.38321703637527804 1102 70-71 0.30261553331148505 1102 72-73 0.33573548247832363 1102 74-75 0.5136325287955614 1102 76-77 0.588496163115714 1102 78-79 0.6168194150942341 1102 80-81 0.5415733629456163 1102 82-83 0.5145613002703051 1102 84-85 0.5649668824278002 1102 86-87 0.5790599648022194 1102 88-89 0.702957649368777 1102 90-91 0.572159098267619 1102 92-93 0.769046291224452 1102 94-95 0.5959394012092147 1102 96-97 0.5118070904027618 1102 98-99 0.5382329400632564 1102 100-101 0.36621674248586444 1102 102-103 0.4239007715475154 1102 104-105 0.3976574270658553 1102 106-107 0.7353968155793815 1102 108-109 0.8313834455751454 1102 110-111 0.6368676894685059 1102 112-113 0.6437574229669139 1102 114-115 0.8041329021875683 1102 116-117 0.8349106800504771 1102 118-119 0.7125901053979895 1102 120-121 0.674772615847381 1102 122-123 0.6363469224623621 1102 124-125 0.47226298697802704 1103 1 0.19378271269806646 1103 2 -1.1198995701930876 1103 3 -0.027366945430422618 1103 4 0.19424781102144806 1103 5 0.5108478069171127 1103 6 0.519871707737515 1103 7 0.47015225816539896 1103 8 0.5485542101776204 1103 9 0.527780680883069 1103 10-11 0.7488441661433214 1103 12-13 0.719316633798897 1103 14-15 0.7109919899300294 1103 16-17 0.8351951807627955 1103 18-19 0.7685578041520742 1103 20-21 0.9184336527444188 1103 22-23 0.8040291918686577 1103 24-25 0.6882990684429018 1103 26-27 0.8035248170896665 1103 28-29 0.7623187609163864 1103 30-31 0.8279551459616883 1103 32-33 0.7392840284070985 1103 34-35 0.7609131135988534 1103 36-37 0.8769546402729276 1103 38-39 0.7896050580695189 1103 40-41 0.8994268162345946 1103 42-43 0.7833257718875473 1103 44-45 0.8198931702588439 1103 46-47 0.8244592796752457 1103 48-49 0.8986144271270504 1103 50-51 0.8535166101369498 1103 52-53 0.887094379347662 1103 54-55 0.9242396037850753 1103 56-57 0.8890006484512654 1103 58-59 0.7327781664609994 1103 60-61 0.7561791537896454 1103 62-63 0.7410412723579789 1103 64-65 0.7692062617875557 1103 66-67 0.6588195350054704 1103 68-69 0.6158015543298383 1103 70-71 0.6188140980830497 1103 72-73 0.5223006011556706 1103 74-75 0.46585480294672976 1103 76-77 0.43669140393431505 1103 78-79 0.6168117821650512 1103 80-81 0.6418176542184462 1103 82-83 0.6180059160802678 1103 84-85 0.6755431566937133 1103 86-87 0.5982177098572521 1103 88-89 0.6166308157249034 1103 90-91 0.6763594991966073 1103 92-93 0.7142759576172679 1103 94-95 0.5946813756064913 1103 96-97 0.6144072664981906 1103 98-99 0.6173279174144177 1103 100-101 0.6958491687706889 1103 102-103 0.6200045491029371 1103 104-105 0.6557122476391513 1103 106-107 0.725165624790499 1103 108-109 0.6562218292485831 1103 110-111 0.6825947850970344 1103 112-113 0.6579498537414104 1103 114-115 0.6727319324677055 1103 116-117 0.6939442110704164 1103 118-119 0.8664276012116083 1103 120-121 0.7583406773943153 1103 122-123 0.8159764300902559 1103 124-125 0.6598238900886919 1104 1 0.2579065065044617 1104 2 -0.790988535348415 1104 3 1.0946441357617687E-4 1104 4 0.3034816326102643 1104 5 0.617523579356984 1104 6 0.595731106998656 1104 7 0.6377844882558179 1104 8 0.6680409063660164 1104 9 0.6896350544578453 1104 10-11 0.8226929865874055 1104 12-13 0.7536074394331749 1104 14-15 0.828984405387267 1104 16-17 0.951406431472158 1104 18-19 0.816553438050569 1104 20-21 1.0083356830946357 1104 22-23 0.9640675647792563 1104 24-25 0.9065523248093541 1104 26-27 0.862902108630081 1104 28-29 1.0719652546068943 1104 30-31 0.9669591144618295 1104 32-33 0.9464419212352979 1104 34-35 0.98460515068588 1104 36-37 0.9755342911673637 1104 38-39 1.0331632854963928 1104 40-41 1.04157505218663 1104 42-43 0.9955948574211462 1104 44-45 0.921870006051094 1104 46-47 0.9748411800508663 1104 48-49 0.9974915804725768 1104 50-51 1.0387926108793692 1104 52-53 0.7995506039204763 1104 54-55 1.0340361403001666 1104 56-57 1.1084708332846702 1104 58-59 1.1302684839701946 1104 60-61 1.0789253181286043 1104 62-63 1.1542089320345497 1104 64-65 1.0697236341361211 1104 66-67 1.0887742563867562 1104 68-69 1.0532426445288436 1104 70-71 1.0575343680967961 1104 72-73 1.0793355033285295 1104 74-75 1.0704834219622086 1104 76-77 1.0974078973869972 1104 78-79 1.0655782398845268 1104 80-81 1.0234543697590972 1104 82-83 1.0703431173180427 1104 84-85 0.9798829480843203 1104 86-87 1.0648563587602098 1104 88-89 1.062818825724115 1104 90-91 0.9888762923126855 1104 92-93 0.9469866781190248 1104 94-95 0.8925827631378027 1104 96-97 0.8924356952139405 1104 98-99 0.8598465939054343 1104 100-101 0.952069040903627 1104 102-103 0.9319335715133583 1104 104-105 0.9475681754794998 1104 106-107 0.9786153895673415 1104 108-109 0.8942350295149275 1104 110-111 0.7734342803823573 1104 112-113 0.7936844000849455 1104 114-115 0.8182783779033329 1104 116-117 0.8426136870891803 1104 118-119 0.7504949136405408 1104 120-121 0.7721287454906474 1104 122-123 0.7809359071019379 1104 124-125 0.7311524468652415 1105 1 0.7392987932002946 1105 2 0.5010277358783988 1105 3 0.6051579188673095 1105 4 0.5869608231204779 1105 5 0.7823179220689411 1105 6 0.6895683719762786 1105 7 0.7482125683585323 1105 8 0.7753946812480308 1105 9 0.762278972681969 1105 10-11 0.870304110850789 1105 12-13 0.9025719636175609 1105 14-15 1.0087877493424742 1105 16-17 1.0701112194993527 1105 18-19 1.022696795648649 1105 20-21 1.0709327820243146 1105 22-23 0.932159900324109 1105 24-25 1.0022208532095647 1105 26-27 0.9910591400812976 1105 28-29 1.1169509557380195 1105 30-31 1.0676238351557998 1105 32-33 1.105846895121168 1105 34-35 1.031248402258825 1105 36-37 1.158664839331827 1105 38-39 1.1041196370311823 1105 40-41 0.9322267702779072 1105 42-43 1.1480316744304773 1105 44-45 1.0973593314020391 1105 46-47 1.045628508643567 1105 48-49 1.050115750910642 1105 50-51 0.9753457979754856 1105 52-53 0.9043056435031893 1105 54-55 0.8549066187397827 1105 56-57 1.004291216059606 1105 58-59 0.8809292952900059 1105 60-61 0.8875072295632478 1105 62-63 0.856314226852195 1105 64-65 0.8845834497879501 1105 66-67 0.8396708102310626 1105 68-69 0.7972386688284487 1105 70-71 0.7928910083201153 1105 72-73 0.8153889622653381 1105 74-75 0.8447370945953274 1105 76-77 0.7499296192254619 1105 78-79 0.8232886029455635 1105 80-81 0.6074470620131613 1105 82-83 0.6774583053940368 1105 84-85 0.6285036773048489 1105 86-87 0.7109814730156074 1105 88-89 0.8005938314368848 1105 90-91 0.6947037833707448 1105 92-93 0.5857717608186306 1105 94-95 0.606491917028201 1105 96-97 0.6085385768705294 1105 98-99 0.6878010580476364 1105 100-101 0.6650163737663846 1105 102-103 0.25868009350171306 1105 104-105 0.20172212760261843 1105 106-107 0.5497630341633517 1105 108-109 0.645496075009877 1105 110-111 0.5817141835848005 1105 112-113 0.40560288370285136 1105 114-115 0.5526331653495689 1105 116-117 0.3143958727367675 1105 118-119 0.21257885577115232 1105 120-121 0.12104256884406084 1105 122-123 0.22650280387702892 1105 124-125 0.15303534428520393 1106 1 0.48617476125947334 1106 2 0.4985808788283137 1106 3 0.5552710301675212 1106 4 0.3332890796249046 1106 5 0.5912278221185829 1106 6 0.6321112604969201 1106 7 0.6460289067714982 1106 8 0.6130223484260213 1106 9 0.659012017883569 1106 10-11 0.7484192203184605 1106 12-13 0.6068183377110827 1106 14-15 0.7398991069393404 1106 16-17 0.8890906261980476 1106 18-19 0.7959582080166641 1106 20-21 0.8066197937959174 1106 22-23 0.6764412948331824 1106 24-25 0.747675815364282 1106 26-27 0.8234449417555965 1106 28-29 1.0841536939225662 1106 30-31 0.8212001140202929 1106 32-33 0.7828908062855291 1106 34-35 0.6436943075784427 1106 36-37 0.8087676052285246 1106 38-39 0.8772828438776727 1106 40-41 0.8248415984252446 1106 42-43 0.8094400885675981 1106 44-45 0.7512612630416697 1106 46-47 0.5628955352882841 1106 48-49 0.5788745730788705 1106 50-51 0.7179853407902925 1106 52-53 0.6316820421737468 1106 54-55 0.6049144331934002 1106 56-57 0.5192651087865201 1106 58-59 0.5833361819930225 1106 60-61 0.6012040772826452 1106 62-63 0.587168757182539 1106 64-65 0.5120239503048687 1106 66-67 0.4484590847250587 1106 68-69 0.5072851170076547 1106 70-71 0.5148620211292858 1106 72-73 0.6141313250948528 1106 74-75 0.5950553877880331 1106 76-77 0.5789987685090523 1106 78-79 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34-35 0.5169280811071104 2204 36-37 0.5002851327980551 2204 38-39 0.6249972671529989 2204 40-41 0.6329811999567596 2204 42-43 0.7463587785279344 2204 44-45 0.7330390102503301 2204 46-47 0.7492123956867474 2204 48-49 0.6603759945429815 2204 50-51 0.7657631567794709 2204 52-53 0.7188619834810375 2204 54-55 0.7557247250520831 2204 56-57 0.748709820710765 2204 58-59 0.7191387360430461 2204 60-61 0.6684153137271522 2204 62-63 0.7485103785686924 2204 64-65 0.6523208528887245 2204 66-67 0.6477868124459008 2204 68-69 0.7482768748085142 2204 70-71 0.6760949119746016 2204 72-73 0.6187887407813939 2204 74-75 0.545508135569861 2204 76-77 0.622856667951929 2204 78-79 0.6012052696122296 2204 80-81 0.6513957054240116 2204 82-83 0.7071198502445348 2204 84-85 0.6633624812915571 2204 86-87 0.684658798596999 2204 88-89 0.5579339477159557 2204 90-91 0.6992185951093965 2204 92-93 0.6606776301431054 2204 94-95 0.64662342311901 2204 96-97 0.6820172590410714 2204 98-99 0.5890005320232738 2204 100-101 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100-101 0.4765523165217296 2206 102-103 0.44966351327552445 2206 104-105 0.4108936323042016 2206 106-107 0.455826369823928 2206 108-109 0.40945711307139376 2206 110-111 0.23557659430452915 2206 112-113 0.11709808237743147 2206 114-115 0.10844433099391182 2206 116-117 0.2490006707375514 2206 118-119 0.038046226567566066 2206 120-121 0.1522923956860467 2206 122-123 0.35584413503272927 2206 124-125 0.1718335514763858 2207 1 0.21183097409822338 2207 2 0.3458851393697664 2207 3 0.4399275385191146 2207 4 0.36063908090557106 2207 5 0.25045829945588594 2207 6 0.033179620098245266 2207 7 0.2256741311361523 2207 8 0.14018205478171097 2207 9 0.16902340398548432 2207 10-11 0.03407376433898435 2207 12-13 0.08291017529433731 2207 14-15 0.16537483578920842 2207 16-17 0.37837250291107694 2207 18-19 0.35657196528473634 2207 20-21 0.387590086913292 2207 22-23 0.38192232463507025 2207 24-25 0.4131533032997652 2207 26-27 0.3537040915113323 2207 28-29 0.48397754840974017 2207 30-31 0.36013672363834814 2207 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-0.04227811096048484 2209 86-87 -0.12615244923388858 2209 88-89 -0.13235040312802582 2209 90-91 -0.03639439026459357 2209 92-93 -0.06297588663511533 2209 94-95 -0.014912195387740468 2209 96-97 -0.11062116973318581 2209 98-99 0.06331278271626317 2209 100-101 0.1138085384574623 2209 102-103 0.2193913099653173 2209 104-105 0.12013778400429231 2209 106-107 0.12290509585600873 2209 108-109 0.15041647364952482 2209 110-111 0.13738073606355172 2209 112-113 0.17237704293125233 2209 114-115 0.22417121927561467 2209 116-117 0.17623651776550986 2209 118-119 0.16836732334177995 2209 120-121 0.13670901355509812 2209 122-123 0.08702759366120105 2209 124-125 0.11578428770302551 2210 1 -0.13491119820666952 2210 2 -0.0592872525974677 2210 3 0.007424081785444514 2210 4 -0.1741387805439416 2210 5 -0.1638871920618712 2210 6 -0.19145187354423854 2210 7 -0.3516828405571033 2210 8 -0.49761456081706257 2210 9 -0.9206366335093819 2210 10-11 -0.6145594815298026 2210 12-13 -0.46410319140299805 2210 14-15 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-2.264960096592386 2216 112-113 -2.3012077475263872 2216 114-115 -2.3563957659904133 2216 116-117 -2.350097482744964 2216 118-119 -2.4659683479940746 2216 120-121 -2.455493976006437 2216 122-123 -2.4254247985592947 2216 124-125 -2.5110496501777 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 97367.0 3 32209.0 4 15071.0 5 11829.0 6 12708.0 7 15325.0 8 17325.0 9 18610.0 10 20260.0 11 22507.0 12 25927.0 13 29050.0 14 31578.0 15 26779.0 16 16381.0 17 14204.0 18 12873.0 19 12067.0 20 11794.0 21 12536.0 22 14498.0 23 19370.0 24 26605.0 25 35587.0 26 42807.0 27 46829.0 28 54512.0 29 63156.0 30 73788.0 31 87120.0 32 105370.0 33 130511.0 34 168579.0 35 231695.0 36 365577.0 37 562028.0 38 213909.0 39 727.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.561389863772504 19.783108116374105 13.451552619803794 24.203949400049602 2 15.611661184064166 20.69501997763454 38.943150504112694 24.7501683341886 3 19.22067172816691 25.628068651845748 29.02646394977822 26.12479567020912 4 12.814723060750893 17.022269929944002 36.49196080836181 33.671046200943294 5 13.200917477773697 39.06099729937772 33.38127768675228 14.356807536096316 6 31.744385082213807 37.251895104221475 16.86057368120753 14.14314613235719 7 28.359254645866383 33.27647415809765 20.376307952454663 17.987963243581298 8 26.543023941235987 35.867171878282385 18.86952046762826 18.720283712853366 9 26.721095673998008 16.234817348764434 19.70485181643474 37.339235160802815 10-11 25.024680122260673 27.39163637644276 26.476794382978408 21.106889118318158 12-13 26.051439139084355 24.59490344045374 27.663655082386583 21.690002338075313 14-15 22.9066531794906 25.617408831877164 25.986916698161444 25.48902129047079 16-17 22.078706082355986 28.99648799713524 26.475196896804054 22.449609023704724 18-19 21.973101065331488 26.994134267187242 29.54081152517204 21.491953142309228 20-21 22.74398817096666 26.070542524066866 29.675963262707068 21.50950604225941 22-23 22.51354481456346 26.003746581952637 29.537042749608013 21.94566585387589 24-25 22.260443234479457 26.218013032646827 29.63673016018863 21.88481357268509 26-27 21.954004122904646 26.510021236960313 29.641472537927903 21.894502102207134 28-29 21.803175106847963 26.38906985972747 29.84406934724277 21.9636856861818 30-31 21.92501669558097 26.22242681963308 29.848067897523865 22.004488587262085 32-33 21.785688548330295 26.275762426664006 29.677826440473744 22.260722584531955 34-35 22.308260167631968 26.22092630139854 29.59247962490749 21.878333906062 36-37 22.369524261179336 25.99377932728556 29.372041799660252 22.264654611874853 38-39 22.170189858128804 26.30130104169291 29.46911674696599 22.059392353212292 40-41 22.236920714283727 26.150994741702462 29.51719436434991 22.094890179663903 42-43 22.094216647752052 26.23436857972909 29.193477810617452 22.477936961901406 44-45 22.30492568266633 26.433592342540525 28.891052187468453 22.370429787324696 46-47 22.491887472946665 26.721331245450976 28.273868020665667 22.5129132609367 48-49 22.57073192780905 26.907309348722375 27.953912912031726 22.568045811436853 50-51 22.530686208818683 26.939526415639254 27.85303149677044 22.67675587877162 52-53 22.920247767236475 26.90855409029533 27.670336270090516 22.50086187237768 54-55 22.584999711011356 26.868200430667176 27.82012161234915 22.726678245972312 56-57 22.422026921208012 27.174429131710365 27.78570553916795 22.617838407913673 58-59 22.690433397911207 26.712174984911453 27.757276866413466 22.840114750763878 60-61 22.74090537919015 26.7775580311426 27.908892995656277 22.57264359401097 62-63 22.59068685931588 26.754198115794043 28.06172352826976 22.593391496620317 64-65 22.445644330980716 26.606865411793734 28.053707015223683 22.893783242001867 66-67 22.354642417308494 27.004747564714933 27.80317131691384 22.837438701062737 68-69 22.456041030717834 26.627748049221744 28.08524181277049 22.830969107289935 70-71 22.251384552000914 26.71392495483626 28.070578436704817 22.964112056458006 72-73 22.646442041926328 26.766585649606156 27.701110032530323 22.885862275937193 74-75 22.444945986646577 26.95276802573982 27.80032000640235 22.801965981211247 76-77 22.617128075244143 26.794499109688065 27.840828675528538 22.74754413953926 78-79 22.57490239143757 26.848504990281754 27.738560217202675 22.83803240107801 80-81 22.257352250566456 26.99505246440329 27.90178881560922 22.84580646942104 82-83 22.55795389771304 27.064053883874905 27.59833179174925 22.779660426662804 84-85 22.414613949645528 26.938921441313934 27.580199068936835 23.066265540103704 86-87 22.458263640743954 26.774403119906204 27.853410202198507 22.913923037151328 88-89 22.590279311228915 26.86694073658018 27.64154145060443 22.901238501586473 90-91 22.400750925875155 27.004747564714933 27.865415024741875 22.72908648466804 92-93 22.351881922374 26.990786069522706 27.814102213770227 22.843229794333066 94-95 22.340092262711412 26.6996637733526 27.77666725270279 23.1835767112332 96-97 22.554846215694486 26.78140434471694 27.505066860325094 23.15868257926348 98-99 22.225027745971207 26.700470037407737 27.49691973869973 23.57758247792132 100-101 22.10826850494378 26.786268052191716 27.757461980127076 23.34800146273743 102-103 22.426864992025948 26.75775124473591 27.563640984762692 23.25174277847545 104-105 22.31674489456231 26.461727302316717 27.83422441566198 23.387303387458996 106-107 22.165633131517023 26.666651846302447 27.79744563612523 23.3702693860553 108-109 22.161202935422086 26.665986158765705 27.397915015184527 23.774895890627683 110-111 22.09274861122355 26.50098729237359 27.722213957992974 23.684050138409887 112-113 22.250660896596315 26.513670879978957 27.770865714333354 23.46480250909138 114-115 22.118033512093398 26.692467050047693 27.532316397347145 23.657183040511764 116-117 21.875134887096838 26.596836292034524 27.685418003187458 23.84261081768118 118-119 21.861790319801855 26.64992599151913 27.53152538981814 23.95675829886088 120-121 21.788030146955293 26.668865190938128 27.725620465543688 23.81748419656289 122-123 21.81929062500463 26.699652322370966 27.52471979050422 23.95633726212018 124-125 21.68229243758877 26.44847830512889 27.635078520355677 24.234150736926658 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 266.0 1 212.0 2 309.0 3 572.0 4 483.0 5 419.5 6 1052.5 7 2318.0 8 3572.0 9 4083.0 10 3369.0 11 2394.0 12 2275.0 13 2505.5 14 2765.0 15 3069.5 16 3355.5 17 3632.5 18 3964.0 19 4315.5 20 4772.0 21 5470.0 22 6313.0 23 7588.0 24 9156.0 25 11188.0 26 14227.5 27 17960.5 28 20869.0 29 24219.0 30 29554.0 31 35047.0 32 41363.0 33 47812.5 34 54669.5 35 59793.5 36 64924.5 37 72116.0 38 76591.0 39 78434.5 40 78800.5 41 81129.0 42 84739.0 43 88561.5 44 92344.0 45 94654.0 46 93938.5 47 94146.0 48 100906.5 49 103670.5 50 101004.5 51 97056.0 52 92211.0 53 90799.5 54 89521.0 55 83518.0 56 77288.0 57 72924.5 58 68757.5 59 62590.5 60 52879.0 61 42206.0 62 32568.0 63 24399.5 64 17990.5 65 13824.0 66 10228.0 67 7336.5 68 5344.5 69 3794.0 70 2783.0 71 2220.0 72 1855.5 73 1766.0 74 1394.0 75 969.0 76 719.5 77 479.5 78 321.5 79 181.0 80 117.5 81 93.5 82 62.5 83 41.0 84 28.0 85 15.5 86 7.0 87 4.0 88 4.5 89 1.5 90 2.0 91 3.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05909447261054557 2 0.07602624313281474 3 0.0 4 0.008410310522002409 5 0.029676910696581193 6 2.9639860870493077E-4 7 7.409965217623269E-5 8 3.7049826088116346E-5 9 6.298470434979778E-4 10-11 0.05753837991484468 12-13 0.009892303565527064 14-15 0.007706363826328199 16-17 0.05416684574082609 18-19 7.409965217623269E-5 20-21 0.010540675522069099 22-23 0.012652515609091732 24-25 0.0 26-27 0.0 28-29 0.05285157691469796 30-31 1.8524913044058173E-5 32-33 0.0038346570001200416 34-35 1.8524913044058173E-5 36-37 1.1114947826434903E-4 38-39 0.0 40-41 1.8524913044058173E-5 42-43 0.003593833130547285 44-45 1.8524913044058173E-5 46-47 5.557473913217451E-5 48-49 1.4819930435246539E-4 50-51 5.001726521895706E-4 52-53 9.262456522029086E-5 54-55 0.0 56-57 0.001611667434833061 58-59 3.7049826088116346E-5 60-61 0.0 62-63 0.0 64-65 6.113221304539197E-4 66-67 0.0 68-69 6.298470434979778E-4 70-71 0.0 72-73 0.0017783916522295844 74-75 0.0022044646522429226 76-77 5.186975652336288E-4 78-79 8.150961739385596E-4 80-81 0.0017598667391855265 82-83 1.6672421739652354E-4 84-85 1.1114947826434903E-4 86-87 4.631228261014542E-4 88-89 0.0 90-91 0.0 92-93 0.006131746217583254 94-95 0.005353699869732812 96-97 0.005853872521922382 98-99 0.0010188702174231993 100-101 7.039466956742105E-4 102-103 5.557473913217451E-5 104-105 3.5197334783710526E-4 106-107 0.002926936260961191 108-109 0.0038346570001200416 110-111 7.224716087182686E-4 112-113 0.0030751355653136562 114-115 4.631228261014542E-4 116-117 0.0019080660435379917 118-119 0.001963640782670166 120-121 0.010670349913377506 122-123 5.927972174098615E-4 124-125 0.002019215521802341 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2699068.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.2933668955784 #Duplication Level Percentage of deduplicated Percentage of total 1 74.86077122354423 36.152786907845794 2 12.51087501281774 12.083845543574625 3 4.827344726994008 6.993861900964722 4 2.427853838612345 4.689969447877775 5 1.3624162848786507 3.289783475507777 6 0.8387534565656966 2.4303737047717062 7 0.5721063311844483 1.93402586686217 8 0.40553279465789704 1.5667635232482464 9 0.310373629814954 1.3490088821429402 >10 1.6481858027215714 14.756866234460755 >50 0.1466278158426574 4.9013293055475105 >100 0.08428341216342304 7.305885758470719 >500 0.0042565374782723596 1.3122364168863672 >1k 3.8695795256866983E-4 0.34407429375723375 >5k 2.3217477154120194E-4 0.8891887380815064 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9991 0.3701648124463704 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8314 0.3080322540965993 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5486 0.20325534591940625 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 3.704982608811634E-5 8 0.0 0.0 0.0 0.0 3.704982608811634E-5 9 0.0 0.0 0.0 3.704982608811634E-5 3.704982608811634E-5 10-11 0.0 0.0 0.0 3.704982608811634E-5 3.704982608811634E-5 12-13 0.0 0.0 0.0 3.704982608811634E-5 3.704982608811634E-5 14-15 0.0 0.0 0.0 3.704982608811634E-5 3.704982608811634E-5 16-17 0.0 0.0 0.0 9.262456522029085E-5 3.704982608811634E-5 18-19 0.0 0.0 0.0 1.296743913084072E-4 3.704982608811634E-5 20-21 0.0 0.0 0.0 2.2229895652869805E-4 3.704982608811634E-5 22-23 0.0 0.0 0.0 3.890231739252216E-4 3.704982608811634E-5 24-25 0.0 0.0 0.0 5.742723043658033E-4 3.704982608811634E-5 26-27 0.0 0.0 0.0 8.521460000266758E-4 3.704982608811634E-5 28-29 0.0 0.0 0.0 0.0014264183043924791 3.704982608811634E-5 30-31 0.0 0.0 0.0 0.0030936604783577144 3.704982608811634E-5 32-33 0.0 0.0 0.0 0.006613393956728768 3.704982608811634E-5 34-35 0.0 0.0 0.0 0.011151997652523019 3.704982608811634E-5 36-37 0.0 0.0 0.0 0.018913936217983394 3.704982608811634E-5 38-39 0.0 0.0 0.0 0.03325221891408442 3.704982608811634E-5 40-41 0.0 0.0 0.0 0.05192533126249506 3.704982608811634E-5 42-43 0.0 0.0 0.0 0.06852365334997118 3.704982608811634E-5 44-45 0.0 0.0 0.0 0.08608527091573832 3.704982608811634E-5 46-47 0.0 0.0 0.0 0.10961191048169219 3.704982608811634E-5 48-49 0.0 0.0 0.0 0.13810322674345366 3.704982608811634E-5 50-51 0.0 0.0 0.0 0.16878048274441398 3.704982608811634E-5 52-53 0.0 0.0 0.0 0.19964298787581491 3.704982608811634E-5 54-55 0.0 0.0 0.0 0.23272848257250278 3.704982608811634E-5 56-57 0.0 0.0 0.0 0.27201982313895023 3.704982608811634E-5 58-59 0.0 0.0 0.0 0.31590534214032395 3.704982608811634E-5 60-61 0.0 0.0 0.0 0.36701557722888045 3.704982608811634E-5 62-63 0.0 0.0 0.0 0.41886680883919936 3.704982608811634E-5 64-65 0.0 0.0 0.0 0.4707736151886503 3.704982608811634E-5 66-67 0.0 0.0 0.0 0.533424871103655 3.704982608811634E-5 68-69 0.0 1.852491304405817E-5 0.0 0.6073763239755352 3.704982608811634E-5 70-71 0.0 3.704982608811634E-5 0.0 0.6949250630217542 3.704982608811634E-5 72-73 0.0 3.704982608811634E-5 0.0 0.7839557951114977 3.704982608811634E-5 74-75 1.852491304405817E-5 3.704982608811634E-5 0.0 0.8710228864185712 7.409965217623268E-5 76-77 3.704982608811634E-5 3.704982608811634E-5 0.0 0.967778507247687 7.409965217623268E-5 78-79 3.704982608811634E-5 3.704982608811634E-5 0.0 1.0819660712512615 7.409965217623268E-5 80-81 3.704982608811634E-5 3.704982608811634E-5 0.0 1.21397460160322 7.409965217623268E-5 82-83 3.704982608811634E-5 3.704982608811634E-5 0.0 1.3590061458251514 1.1114947826434903E-4 84-85 3.704982608811634E-5 3.704982608811634E-5 0.0 1.503759816351422 1.1114947826434903E-4 86-87 3.704982608811634E-5 3.704982608811634E-5 0.0 1.6581093918345147 1.1114947826434903E-4 88-89 3.704982608811634E-5 3.704982608811634E-5 0.0 1.8376343241444824 1.1114947826434903E-4 90-91 3.704982608811634E-5 3.704982608811634E-5 0.0 2.0479106121075867 1.1114947826434903E-4 92-93 3.704982608811634E-5 3.704982608811634E-5 0.0 2.2740257007233606 1.1114947826434903E-4 94-95 3.704982608811634E-5 3.704982608811634E-5 0.0 2.5002889886434874 1.1114947826434903E-4 96-97 3.704982608811634E-5 3.704982608811634E-5 0.0 2.725403731954882 1.1114947826434903E-4 98-99 3.704982608811634E-5 3.704982608811634E-5 0.0 2.9777686223540867 1.1114947826434903E-4 100-101 3.704982608811634E-5 3.704982608811634E-5 0.0 3.2762975960590843 1.1114947826434903E-4 102-103 7.409965217623268E-5 3.704982608811634E-5 0.0 3.603743959025856 1.1114947826434903E-4 104-105 7.409965217623268E-5 3.704982608811634E-5 0.0 3.9301158770360733 1.1114947826434903E-4 106-107 7.409965217623268E-5 3.704982608811634E-5 0.0 4.262156418437772 1.1114947826434903E-4 108-109 7.409965217623268E-5 3.704982608811634E-5 0.0 4.611591853187841 1.1114947826434903E-4 110-111 7.409965217623268E-5 3.704982608811634E-5 0.0 5.012674745504745 1.1114947826434903E-4 112-113 7.409965217623268E-5 3.704982608811634E-5 0.0 5.458847276170886 1.1114947826434903E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7960 0.0 48.511776 1 GTATCAA 13095 0.0 45.68919 1 ATCAACG 16620 0.0 35.76575 3 TCAACGC 16845 0.0 35.255314 4 TATCAAC 17020 0.0 34.883064 2 CAACGCA 16885 0.0 34.85231 5 AACGCAG 17370 0.0 34.00625 6 GTACATG 14260 0.0 31.634542 1 TACATGG 14415 0.0 30.900534 2 ACGCAGA 20265 0.0 28.824995 7 ACATGGG 15030 0.0 28.74157 3 CGCAGAG 20260 0.0 28.596985 8 GCAGAGT 21720 0.0 25.963375 9 GAGTACT 11965 0.0 25.06098 12-13 AGTACTT 12660 0.0 24.249693 12-13 CATGGGA 9760 0.0 24.205038 4 GTACTTT 12625 0.0 23.609346 14-15 AGAGTAC 20960 0.0 22.455584 10-11 CATGGGG 5320 0.0 22.370897 4 AGTACAT 10755 0.0 21.261793 2 >>END_MODULE