FastQCFastQC Report
Thu 26 May 2016
SRR1364593_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364593_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1668749
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA78870.4726294967068145No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA45740.27409754253036256No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA43040.2579177575537124No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27750.16629223448223787No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT24310.1456779899193947No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG145800.033.0556261
TACATGG144400.032.3024832
GTTATCA12000.032.2690731
CGCATAG2601.8189894E-1229.7465928
ACATGGG150200.029.508313
ACGCATA2651.8189894E-1229.1914717
CATGGGG40450.027.981854
TACCTGG18300.027.0189442
GTGTACG2006.046321E-826.8081531
AACGCAT2905.456968E-1226.6797736
ATGGGAT35300.025.990435
ATGGGAG34050.025.7198055
CATGGGA108000.025.3732094
GTATAGA6450.024.9378171
CCTATAC4250.023.8287563
GAGTACT37800.023.45031212-13
AGTACTT41000.022.92595312-13
TTATCAA16850.022.2731672
CGCAGAT7700.021.6338848
ACCTGGG22900.021.5915573
Produced by FastQC (version 0.11.5)