##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364593_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1668749 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.993441194571503 31.0 26.0 31.0 7.0 34.0 2 25.79686519662334 31.0 25.0 31.0 7.0 34.0 3 26.223628598429123 31.0 26.0 31.0 7.0 34.0 4 29.335945070229254 35.0 28.0 37.0 7.0 37.0 5 29.820271952222893 35.0 30.0 37.0 7.0 37.0 6 29.727572570830006 35.0 30.0 37.0 2.0 37.0 7 29.656024962411962 35.0 30.0 37.0 2.0 37.0 8 29.380845771293345 35.0 29.0 37.0 2.0 37.0 9 30.1458086267018 37.0 28.0 39.0 2.0 39.0 10-11 30.12799168718603 36.0 27.5 39.0 2.0 39.0 12-13 30.120504941126555 37.0 27.5 39.0 2.0 39.0 14-15 30.686134943002216 37.0 27.0 40.0 2.0 41.0 16-17 31.092217583351363 37.0 29.0 40.0 2.0 41.0 18-19 30.923300328569486 37.0 27.5 40.0 2.0 41.0 20-21 30.844923352763058 37.0 27.0 40.0 2.0 41.0 22-23 30.915406690880417 37.0 27.5 40.0 2.0 41.0 24-25 31.02531237471903 37.0 28.0 40.0 2.0 41.0 26-27 30.832807390446376 37.0 27.5 40.0 2.0 41.0 28-29 30.73379969066648 37.0 28.0 40.0 2.0 41.0 30-31 30.601589274360613 37.0 27.5 40.0 2.0 41.0 32-33 30.485521189825434 37.0 28.0 40.0 2.0 41.0 34-35 30.507301577409184 37.0 28.0 40.0 2.0 41.0 36-37 30.409004290040023 37.0 28.0 40.0 2.0 41.0 38-39 30.088305371269136 36.0 27.0 40.0 2.0 41.0 40-41 29.93896925181678 36.0 27.0 40.0 2.0 41.0 42-43 29.42529883163975 35.5 25.0 40.0 2.0 41.0 44-45 29.27049843925 35.0 25.0 40.0 2.0 41.0 46-47 29.180894490423665 35.0 25.0 40.0 2.0 41.0 48-49 29.067461913085793 35.0 25.0 40.0 2.0 41.0 50-51 28.81903539717477 35.0 24.0 40.0 2.0 41.0 52-53 28.698300043925123 35.0 24.0 40.0 2.0 41.0 54-55 28.534352979387556 35.0 24.0 39.0 2.0 41.0 56-57 28.323949857048603 35.0 23.5 39.0 2.0 41.0 58-59 28.111433475016312 34.0 23.0 39.0 2.0 40.0 60-61 27.799299954636677 34.0 22.0 39.0 2.0 40.0 62-63 27.599423580178925 34.0 22.0 38.5 2.0 40.0 64-65 27.34901144510049 34.0 21.5 38.0 2.0 40.0 66-67 27.073994950708585 33.5 21.0 38.0 2.0 40.0 68-69 26.84171496132732 33.0 20.0 37.5 2.0 40.0 70-71 26.395600836315108 33.0 18.0 37.0 2.0 39.5 72-73 26.124581348063728 33.0 18.0 36.0 2.0 39.0 74-75 26.085770238663812 33.0 18.0 36.0 2.0 39.0 76-77 25.812032846162005 33.0 17.0 36.0 2.0 39.0 78-79 25.535065189552174 32.0 14.0 35.0 2.0 37.5 80-81 25.148238291079124 32.0 10.5 35.0 2.0 37.0 82-83 25.263009595810992 32.5 12.5 35.0 2.0 37.0 84-85 25.097295039577553 32.0 9.0 35.0 2.0 36.0 86-87 24.952153679193216 32.0 7.5 35.0 2.0 36.0 88-89 24.76101738487933 32.0 7.0 35.0 2.0 36.0 90-91 24.610255946220793 32.0 6.0 35.0 2.0 35.0 92-93 24.494828161694777 32.0 2.0 35.0 2.0 35.0 94-95 24.41061957190686 32.0 2.0 35.0 2.0 35.0 96-97 24.28870609061039 32.0 2.0 35.0 2.0 35.0 98-99 24.181059883781202 32.0 2.0 35.0 2.0 35.0 100-101 24.057784004664573 32.0 2.0 35.0 2.0 35.0 102-103 22.96072477047177 30.0 2.0 34.0 2.0 35.0 104-105 23.745441045957183 31.5 2.0 34.5 2.0 35.0 106-107 23.894475742007934 32.0 2.0 35.0 2.0 35.0 108-109 23.847213691214197 32.0 2.0 35.0 2.0 35.0 110-111 23.766114017146975 32.0 2.0 35.0 2.0 35.0 112-113 23.65581132932514 32.0 2.0 35.0 2.0 35.0 114-115 23.522743384415513 32.0 2.0 35.0 2.0 35.0 116-117 23.403241589957506 32.0 2.0 35.0 2.0 35.0 118-119 23.300459505893336 32.0 2.0 35.0 2.0 35.0 120-121 23.106050400629453 32.0 2.0 35.0 2.0 35.0 122-123 22.87328981170925 32.0 2.0 35.0 2.0 35.0 124-125 21.948962666045045 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.3574833806855295 1101 2 1.6899558394409482 1101 3 1.2582989775867475 1101 4 1.3255636520122387 1101 5 1.211977375096751 1101 6 1.0817052173299224 1101 7 1.2773015354422554 1101 8 1.3310371189442485 1101 9 1.7029143296366698 1101 10-11 1.800606668816286 1101 12-13 1.7083564056628298 1101 14-15 2.0472992464056396 1101 16-17 1.561693843288385 1101 18-19 1.5252596944475236 1101 20-21 1.6747962642134588 1101 22-23 1.4209017846221634 1101 24-25 1.409861288406745 1101 26-27 1.7164287993520304 1101 28-29 1.7491171076487326 1101 30-31 1.8653675347370324 1101 32-33 1.8598853443138594 1101 34-35 1.5532370169196028 1101 36-37 1.5758997323325232 1101 38-39 1.5291198543608218 1101 40-41 1.5971290815504915 1101 42-43 1.645018705451804 1101 44-45 1.571291736072169 1101 46-47 1.2832680476762377 1101 48-49 1.0540701939743151 1101 50-51 1.149733169600438 1101 52-53 1.0242921662959006 1101 54-55 0.9814977183304983 1101 56-57 1.1104552783676525 1101 58-59 0.8567698577837177 1101 60-61 0.9336078458331016 1101 62-63 0.8788614199638154 1101 64-65 0.8582069919051918 1101 66-67 0.8360173975518137 1101 68-69 0.7963216529107946 1101 70-71 0.6954133259597697 1101 72-73 0.6039948615115662 1101 74-75 0.6075509250469793 1101 76-77 0.6893265402303541 1101 78-79 0.6591754847364975 1101 80-81 0.8599188572307099 1101 82-83 0.9109354015431776 1101 84-85 0.9306883366006815 1101 86-87 0.8044473561080174 1101 88-89 0.8189908819362977 1101 90-91 0.8770611099505423 1101 92-93 0.9055410655643286 1101 94-95 0.975081560935319 1101 96-97 0.8910051595195831 1101 98-99 0.980836217087429 1101 100-101 1.003928508065954 1101 102-103 0.9030838985434251 1101 104-105 1.0352807026458386 1101 106-107 1.0877966436278186 1101 108-109 1.0710002068674243 1101 110-111 1.1188459962264545 1101 112-113 1.0925022800938748 1101 114-115 1.1187065604200015 1101 116-117 1.140462177050722 1101 118-119 0.9891129034567179 1101 120-121 0.9984303209430152 1101 122-123 0.9281813316950789 1101 124-125 0.8436070483596083 1102 1 0.4670338732700685 1102 2 0.8458374010063743 1102 3 0.591168314145829 1102 4 0.39713485328641696 1102 5 0.5317632510437065 1102 6 0.11227956035615705 1102 7 0.2757346017994884 1102 8 0.18604425958849546 1102 9 0.9240058905351809 1102 10-11 0.7761121400248854 1102 12-13 0.8618680191067547 1102 14-15 0.9923911088965802 1102 16-17 0.8018410026523917 1102 18-19 0.6154633448986111 1102 20-21 0.965820062090021 1102 22-23 0.8611914940454177 1102 24-25 0.5785400770302047 1102 26-27 0.9193384106559002 1102 28-29 0.5334777826244128 1102 30-31 0.8093174198000881 1102 32-33 1.005953981888137 1102 34-35 0.9177399659876073 1102 36-37 0.6534157211663754 1102 38-39 0.9244653107460543 1102 40-41 1.0295155416931507 1102 42-43 1.286756387060219 1102 44-45 0.8822158704641225 1102 46-47 0.8575865982716522 1102 48-49 0.8615403065918201 1102 50-51 0.9732249692760426 1102 52-53 0.8488352685518343 1102 54-55 0.6866260708769332 1102 56-57 0.8482709278607423 1102 58-59 0.8621164706017908 1102 60-61 0.7609688496693714 1102 62-63 0.5990043445496092 1102 64-65 0.5804700981166455 1102 66-67 0.5999740142367074 1102 68-69 0.6710601824604474 1102 70-71 0.748455319255747 1102 72-73 0.43049174390661804 1102 74-75 0.499198753137982 1102 76-77 0.4085409510918865 1102 78-79 0.4241463620123582 1102 80-81 0.6075967926564196 1102 82-83 0.5465194313370922 1102 84-85 0.49542960282236237 1102 86-87 0.4993680533975926 1102 88-89 0.4782609231309287 1102 90-91 0.6426313939632173 1102 92-93 0.7119842691066864 1102 94-95 0.6958439327133625 1102 96-97 0.44811561166839553 1102 98-99 0.597431176157265 1102 100-101 0.7214397266143493 1102 102-103 0.5961546318236906 1102 104-105 0.6925205799982486 1102 106-107 0.6691220437880787 1102 108-109 0.6816766609420917 1102 110-111 0.7474762343826242 1102 112-113 0.6542121810316033 1102 114-115 0.8290144672338542 1102 116-117 0.8890930197058751 1102 118-119 0.8022215296243616 1102 120-121 0.7765632601254886 1102 122-123 0.6973579120903253 1102 124-125 0.6533406647123563 1103 1 0.9961577545359219 1103 2 1.2528738188923931 1103 3 0.8067730757080334 1103 4 0.7379434635382829 1103 5 0.8162086473136796 1103 6 0.9384483533409238 1103 7 0.746368840987671 1103 8 1.0047801238244425 1103 9 1.4186528618930794 1103 10-11 1.4955840044509046 1103 12-13 1.5005034422837618 1103 14-15 1.5826257089571634 1103 16-17 1.2383527366152514 1103 18-19 1.3216897467935205 1103 20-21 1.5859746340094212 1103 22-23 1.3752576311069724 1103 24-25 1.2743473442401125 1103 26-27 1.4923749790154162 1103 28-29 1.4489445111524084 1103 30-31 1.4378128762264595 1103 32-33 1.6060896390250363 1103 34-35 1.3338064435944474 1103 36-37 1.1170544277014756 1103 38-39 1.3882419513196531 1103 40-41 1.3165561107997519 1103 42-43 1.5725901983228958 1103 44-45 1.2170391993998457 1103 46-47 1.1274979377035628 1103 48-49 0.9150219201164838 1103 50-51 0.9519639881756454 1103 52-53 0.8505810580988076 1103 54-55 0.8159273524755442 1103 56-57 0.6917085344873861 1103 58-59 0.7731925738926044 1103 60-61 0.8365161145165843 1103 62-63 0.5574022175935376 1103 64-65 0.5900657533924232 1103 66-67 0.5681463948264174 1103 68-69 0.6091155897057448 1103 70-71 0.7106953812402672 1103 72-73 0.4277399928372958 1103 74-75 0.43656432519795985 1103 76-77 0.48514169540771945 1103 78-79 0.4069834737923905 1103 80-81 0.5687508715771301 1103 82-83 0.6466049306418107 1103 84-85 0.7120383459924824 1103 86-87 0.7357977199074064 1103 88-89 0.7469079222076296 1103 90-91 0.6820917484866946 1103 92-93 0.7695025981645074 1103 94-95 0.7265842491168861 1103 96-97 0.6380263637256895 1103 98-99 0.6834988813110492 1103 100-101 0.6007535476710046 1103 102-103 0.5784474321288577 1103 104-105 0.4918569126819534 1103 106-107 0.6102306440743703 1103 108-109 0.7169948694891168 1103 110-111 0.6804765901072543 1103 112-113 0.7772141998843516 1103 114-115 0.6481223903894104 1103 116-117 0.6303668768497701 1103 118-119 0.7206668744165619 1103 120-121 0.6177150555448954 1103 122-123 0.5953669101414292 1103 124-125 0.5488338514968234 1104 1 0.8679941550856363 1104 2 1.2641121331484726 1104 3 0.6617749953308234 1104 4 0.7019577709981881 1104 5 0.4121955745860184 1104 6 0.49656179822459734 1104 7 0.3170331462289191 1104 8 0.6041776773825625 1104 9 1.0865118168851602 1104 10-11 1.1456735868477388 1104 12-13 1.1253359978000894 1104 14-15 1.0953477919504628 1104 16-17 0.7472410402924972 1104 18-19 0.805539995637087 1104 20-21 0.8612126897434358 1104 22-23 0.8179425821003257 1104 24-25 0.8410777089427235 1104 26-27 0.9475525907474278 1104 28-29 0.99216431985894 1104 30-31 1.2029703399578118 1104 32-33 1.1212282923823196 1104 34-35 1.05834282414407 1104 36-37 0.868300253245387 1104 38-39 0.9741315662568262 1104 40-41 0.9609479183512946 1104 42-43 1.2075972369397903 1104 44-45 1.0546090469811489 1104 46-47 0.8242796917050264 1104 48-49 0.6943169072964892 1104 50-51 0.5272591824305444 1104 52-53 0.5497891895823592 1104 54-55 0.7224336840152965 1104 56-57 0.7034725745827437 1104 58-59 0.6327986943060075 1104 60-61 0.5594290150891261 1104 62-63 0.46362044494246746 1104 64-65 0.5464198977519175 1104 66-67 0.4011339066673081 1104 68-69 0.362376858405991 1104 70-71 0.4238503600729686 1104 72-73 0.3193988298867225 1104 74-75 0.3420129349344663 1104 76-77 0.3302326379392895 1104 78-79 0.2331434629370328 1104 80-81 0.3682837312105818 1104 82-83 0.3710265196779048 1104 84-85 0.511144676374677 1104 86-87 0.37547471523793163 1104 88-89 0.3446246000319029 1104 90-91 0.396880496731864 1104 92-93 0.4225441231102778 1104 94-95 0.38338479419972415 1104 96-97 0.4529747268372937 1104 98-99 0.5260692337177773 1104 100-101 0.4892117272255092 1104 102-103 0.3947716856785526 1104 104-105 0.44120170072922704 1104 106-107 0.5206919773408245 1104 108-109 0.5861802934749498 1104 110-111 0.4624050296564981 1104 112-113 0.47670513006860205 1104 114-115 0.4417521687871293 1104 116-117 0.42036496687332914 1104 118-119 0.5015314828102966 1104 120-121 0.5803753376169034 1104 122-123 0.5457194197668755 1104 124-125 0.39618428973030717 1105 1 0.9576112294013086 1105 2 1.289875587678278 1105 3 0.7569209539622861 1105 4 0.9195857673185834 1105 5 0.6842580645085796 1105 6 0.6338862177406774 1105 7 0.656706529947126 1105 8 0.554606762796702 1105 9 0.8422714532491327 1105 10-11 0.9241717366372733 1105 12-13 0.9852319131703986 1105 14-15 1.2913744141039665 1105 16-17 0.9808287523226404 1105 18-19 1.0030956704909073 1105 20-21 0.8746373949850721 1105 22-23 0.9998447867260545 1105 24-25 0.558766441749718 1105 26-27 0.9989901037762579 1105 28-29 1.1492658442360195 1105 30-31 1.0463895804690182 1105 32-33 1.1351969852865267 1105 34-35 0.8097764226985724 1105 36-37 0.5600850298547257 1105 38-39 0.8443835186008961 1105 40-41 0.9609362681300837 1105 42-43 1.086910109999291 1105 44-45 0.9030253780437789 1105 46-47 0.3274535226363966 1105 48-49 0.44090991660455003 1105 50-51 0.6571429163295477 1105 52-53 0.31739826594349907 1105 54-55 0.4561542085672876 1105 56-57 0.40213230206698114 1105 58-59 0.6056571739428165 1105 60-61 0.3672764798301209 1105 62-63 0.3436960968189453 1105 64-65 0.1591833209037965 1105 66-67 0.11483857449153945 1105 68-69 -0.054278468712031014 1105 70-71 0.3899923318433949 1105 72-73 0.2835125758118693 1105 74-75 0.41154782392960243 1105 76-77 0.3567246196357061 1105 78-79 0.42417035382230495 1105 80-81 0.6614810427744651 1105 82-83 0.5718562173655393 1105 84-85 0.2888254466438127 1105 86-87 0.31218108275177414 1105 88-89 0.42682630854461223 1105 90-91 0.14439076679020602 1105 92-93 0.16136426016037575 1105 94-95 0.08516134419952337 1105 96-97 0.1692902092356725 1105 98-99 0.32379374357743984 1105 100-101 0.3119793972713616 1105 102-103 0.1353743065744517 1105 104-105 0.2999281668940377 1105 106-107 0.24154040547744415 1105 108-109 0.20850691721287973 1105 110-111 0.3757008213169897 1105 112-113 0.31224113189287195 1105 114-115 0.37959023878973497 1105 116-117 0.36654653696898265 1105 118-119 0.4798954686523267 1105 120-121 0.3300672280998498 1105 122-123 0.22663469531946134 1105 124-125 0.203083737137014 1106 1 0.42186483945686604 1106 2 -1.3864515148730412 1106 3 0.18683056924624708 1106 4 0.49846544118712544 1106 5 0.5058365628554746 1106 6 0.6874027650271302 1106 7 0.5023054231070603 1106 8 0.6439451566853371 1106 9 1.0678963205896146 1106 10-11 1.3715035236050461 1106 12-13 1.3588587202581657 1106 14-15 1.4017062387619141 1106 16-17 1.1164213144477309 1106 18-19 1.025893041822048 1106 20-21 1.1931075978452164 1106 22-23 0.9935208474032748 1106 24-25 0.9459958945355922 1106 26-27 0.9564041667304579 1106 28-29 1.0216107273972206 1106 30-31 1.219243540478665 1106 32-33 1.2392730791677309 1106 34-35 1.1242017977425753 1106 36-37 0.8942589915550911 1106 38-39 1.2237382413407865 1106 40-41 1.1457145926443673 1106 42-43 1.4515420104496037 1106 44-45 1.2595913508906733 1106 46-47 1.048525736565253 1106 48-49 0.897011995042547 1106 50-51 0.9399572924937267 1106 52-53 0.9388722694082503 1106 54-55 0.7517049666358453 1106 56-57 0.6572918513168311 1106 58-59 0.7104178710494189 1106 60-61 0.8028837212888327 1106 62-63 0.7138322896010187 1106 64-65 0.6617354191863605 1106 66-67 0.7719086649525515 1106 68-69 0.6415125604450651 1106 70-71 0.7668678188974312 1106 72-73 0.565089203382982 1106 74-75 0.6826351368195986 1106 76-77 0.5893935071238019 1106 78-79 0.5903410970558873 1106 80-81 0.7294866416737307 1106 82-83 0.6885089295097551 1106 84-85 0.6122814348124521 1106 86-87 0.6497966004859173 1106 88-89 0.6903515520391998 1106 90-91 0.7280155886583266 1106 92-93 0.6885864129533168 1106 94-95 0.7144796356410588 1106 96-97 0.6999877171517568 1106 98-99 0.6947074680066727 1106 100-101 0.6681554980039408 1106 102-103 0.7005037784495691 1106 104-105 0.801925136115198 1106 106-107 0.8553825287865919 1106 108-109 0.89601228653774 1106 110-111 0.8274628067563725 1106 112-113 0.7007689885224764 1106 114-115 0.7104227446938935 1106 116-117 0.7188555030662798 1106 118-119 0.7155411937694467 1106 120-121 0.778274968442279 1106 122-123 0.7687007520155618 1106 124-125 0.41466420544438165 1107 1 0.8891768997708773 1107 2 -1.0870106780307154 1107 3 0.3898466338469042 1107 4 0.9977557539410462 1107 5 0.8313807828336408 1107 6 0.8223061486484333 1107 7 0.8771603149730574 1107 8 1.0302016186623035 1107 9 1.4064879843283293 1107 10-11 1.651458757783633 1107 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70-71 -4.1775157802877985 2216 72-73 -2.2899570127447717 2216 74-75 -1.9309504664962915 2216 76-77 -1.6898697611540925 2216 78-79 -1.805186740892669 2216 80-81 -1.8134302104751328 2216 82-83 -1.9034112176548312 2216 84-85 -1.8312015551392058 2216 86-87 -1.9098816651189026 2216 88-89 -2.0007320260596266 2216 90-91 -1.8347933323784424 2216 92-93 -1.9275394266864083 2216 94-95 -1.9392752694311035 2216 96-97 -1.8979767000862644 2216 98-99 -2.0216327854986744 2216 100-101 -2.0413612827209633 2216 102-103 -1.8865463340467166 2216 104-105 -1.9223220191736914 2216 106-107 -1.8727526882648462 2216 108-109 -1.9873148344733238 2216 110-111 -1.8873601491588587 2216 112-113 -2.2140552453561924 2216 114-115 -2.177553110316893 2216 116-117 -2.009744182696707 2216 118-119 -2.000969951846681 2216 120-121 -2.060659465643564 2216 122-123 -1.940569523761848 2216 124-125 -1.7471030333949145 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 172519.0 3 23014.0 4 14656.0 5 11688.0 6 10933.0 7 11866.0 8 12747.0 9 13356.0 10 14795.0 11 16125.0 12 17696.0 13 18940.0 14 19171.0 15 15562.0 16 11810.0 17 10462.0 18 9469.0 19 8619.0 20 8356.0 21 8743.0 22 10755.0 23 15850.0 24 23901.0 25 30341.0 26 31889.0 27 32766.0 28 37634.0 29 42342.0 30 48942.0 31 56884.0 32 68316.0 33 85273.0 34 110140.0 35 148282.0 36 206412.0 37 229564.0 38 58840.0 39 91.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.254001262244884 19.28310255229279 13.596875493533547 21.866020691928778 2 12.798707700034061 21.824022049357545 42.39004607032558 22.987224180282816 3 19.146576683738818 29.53887155494034 27.247143905566013 24.067407855754837 4 11.357972161573693 18.253875245724206 37.06245165896516 33.325700933736954 5 12.520327158285816 41.606153605684696 32.13551639248173 13.738002843547761 6 28.890573390651774 39.28826063113156 17.958904430486573 13.862261547730098 7 27.622656286129033 35.914414016728735 19.341649054466593 17.121280642675636 8 25.289925258640412 37.5688371704808 19.026951204646732 18.11428636623206 9 26.43531770550925 19.807093108229257 19.231002490147795 34.5265866961137 10-11 23.230614998735238 30.57695083268795 26.07106496019223 20.121369208384582 12-13 25.130201767718606 27.651727979753595 26.436991708866685 20.781078543661113 14-15 22.23361286265139 28.269404823194076 25.529907435619954 23.967074878534582 16-17 21.10710692926203 31.66465190428058 25.787768091445802 21.440473075011585 18-19 21.158761178303507 30.154717626822524 28.215554014583198 20.470967180290764 20-21 22.80521281561383 28.67216684852187 27.665474476984937 20.85714585887936 22-23 22.076231366249832 28.661762718988786 27.202948373440623 22.059057541320765 24-25 21.879878473473113 28.437597569469464 27.920389317193845 21.762134639863582 26-27 22.25669022291532 28.181174864897983 28.000087705176046 21.56204720701065 28-29 21.561414946188535 28.159742732690766 28.644009902654314 21.634832418466384 30-31 21.510887440431176 28.61503235022267 28.36139219993518 21.512688009410972 32-33 21.460335796957132 28.479036782036392 28.045773627807375 22.0148537931991 34-35 21.964203082865748 27.978870192062427 28.0257439635765 22.031182761495327 36-37 22.281922898602044 27.83398577286702 28.146796644425205 21.73729468410573 38-39 22.400739176663343 27.60589492188274 28.363022675408867 21.630343226045053 40-41 22.002111898177322 27.853757206169483 28.705902416132812 21.438228479520383 42-43 21.640778720477783 28.108393775596134 28.417192372392243 21.83363513153384 44-45 21.807808810093512 28.53714773492642 27.625037368041287 22.030006086938776 46-47 21.725110467426365 28.435353897900605 27.00299861593108 22.83653701874195 48-49 22.14213923415841 28.235475596613423 27.2580136424518 22.364371526776367 50-51 22.480439382343672 28.481429793364242 27.11070094089047 21.92742988340161 52-53 22.242341245993288 28.650767301276442 27.118686297123695 21.988205155606575 54-55 21.70312312876554 28.662381955589517 27.36400224390557 22.270492671739365 56-57 22.12221805357545 29.12160276131162 26.78184137465296 21.974337810459968 58-59 22.10703924776419 28.411749243527222 27.068132361431353 22.413079147277237 60-61 22.11789484172179 28.245100219408613 27.467971774286724 22.169033164582867 62-63 22.590090657794136 28.128342053429645 27.150647015542535 22.130920273233684 64-65 22.319073405228586 28.15208842989763 27.409781622753073 22.119056542120713 66-67 21.92343833255728 28.58521666290641 27.351891810156538 22.139453194379772 68-69 21.944486365517015 28.855982763788475 27.07286535814914 22.12666551254537 70-71 21.84886707311219 28.911898132590853 26.91700470452691 22.322230089770052 72-73 22.17350183561339 28.544440621603872 26.640016101924463 22.642041440858275 74-75 22.07896028288292 28.729216636529763 26.803054645982392 22.388768434604923 76-77 22.484368249243918 28.374927991935095 27.07002184244635 22.070681916374635 78-79 22.32312654714575 28.376595904283246 27.25809016577901 22.04218738279199 80-81 21.785479687761164 29.17163923588928 27.105651129244592 21.937229947104967 82-83 21.95390198830985 29.16757942826414 26.59014073433446 22.28837784909155 84-85 22.212904031099487 28.428810953471988 26.745205515622356 22.61307949980617 86-87 22.35615997618173 28.324263422387734 27.08484898688314 22.234727614547392 88-89 22.78705148978454 27.869104358596292 27.205640716445895 22.138203435173267 90-91 22.170622376955073 28.482434413749267 27.47453699674897 21.872406212546686 92-93 21.800354393382655 28.802369424343315 27.279258084505265 22.11801809776876 94-95 21.7401587517881 29.054710718897205 26.874259771614966 22.330870757699724 96-97 22.066821903592047 28.660359753921767 26.500107589453055 22.772710753033138 98-99 22.19125299339203 28.464262350180352 26.522353661391097 22.82213099503652 100-101 22.050706757460617 28.834900305073436 26.87439543373954 22.239997503726407 102-103 22.015582566351068 28.671069661119102 27.133493333077208 22.179854439452622 104-105 21.693349962452473 28.551181494514584 27.374412297947654 22.381056245085293 106-107 21.697602003800935 29.197753525044213 26.87023720725646 22.234407263898397 108-109 21.693957768384173 29.15199186683259 26.571577664637076 22.582472700146166 110-111 21.566593906113688 28.820169033284653 26.97455500661805 22.63868205398361 112-113 22.20014098660627 28.749658782415672 26.665246801553856 22.384953429424204 114-115 21.811802917943268 28.915823114012646 26.818105190660997 22.454268777383092 116-117 21.301065672738726 29.025625642140945 27.107804684160396 22.56550400095993 118-119 21.426519027472096 29.435969327615403 26.79904525214497 22.33846639276753 120-121 21.509836294193597 29.430306192989008 26.52693865493419 22.532918857883207 122-123 21.403084994237158 29.288425781763266 26.369566926039457 22.938922297960115 124-125 21.515837925286235 29.221709355404705 26.551702104873815 22.710750614435245 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 8288.0 1 6123.5 2 3112.5 3 1827.5 4 1205.0 5 950.5 6 949.0 7 1140.0 8 1488.5 9 1643.5 10 1542.0 11 1479.5 12 1501.5 13 1634.5 14 1748.0 15 1944.5 16 2152.0 17 2345.5 18 2577.5 19 2816.0 20 3315.5 21 3973.5 22 4640.5 23 5485.0 24 6579.0 25 8060.0 26 10219.5 27 12770.0 28 15006.5 29 17516.0 30 20813.0 31 23744.0 32 26815.5 33 30571.5 34 34754.5 35 37897.0 36 41082.0 37 44926.0 38 47566.5 39 49190.0 40 50162.0 41 52390.5 42 54577.0 43 56205.0 44 58275.0 45 59567.5 46 61066.5 47 64719.5 48 75427.0 49 75315.5 50 65815.5 51 60114.5 52 53781.0 53 50556.0 54 47525.0 55 43531.0 56 38831.5 57 34526.0 58 31396.0 59 28419.0 60 24484.5 61 20165.0 62 16297.5 63 12782.0 64 9948.0 65 8069.0 66 6295.0 67 4804.5 68 3693.5 69 2730.5 70 2063.0 71 1637.0 72 1350.0 73 1175.0 74 985.5 75 798.0 76 575.0 77 376.5 78 265.5 79 182.5 80 147.0 81 122.5 82 88.0 83 60.5 84 42.5 85 36.5 86 33.0 87 26.5 88 19.0 89 11.0 90 7.0 91 5.5 92 4.0 93 3.5 94 3.5 95 3.0 96 1.5 97 1.0 98 0.5 99 0.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2074607984783811 2 0.246891533717773 3 0.30118370108386583 4 0.2870413705116827 5 0.31964064098315564 6 0.233588154959194 7 0.222322230605082 8 0.20410499122396475 9 0.2029064886331018 10-11 0.14552817709553684 12-13 0.17216489717746647 14-15 0.1888540457552334 16-17 0.16257687645056265 18-19 0.1878053559882283 20-21 0.18256190715320278 22-23 0.20518364355574142 24-25 0.1958952484765534 26-27 0.2445544536655902 28-29 0.1757604049500554 30-31 0.1563446629780752 32-33 0.19466678332091886 34-35 0.15412743318497868 36-37 0.15379784497249138 38-39 0.18648700313827904 40-41 0.20509375586142672 42-43 0.2731087778929006 44-45 0.17300384899107055 46-47 0.15862181790071483 48-49 0.16176788720173016 50-51 0.16623230935269476 52-53 0.18612745236102013 54-55 0.17399261362853252 56-57 0.17222482230700964 58-59 0.14759559406477546 60-61 0.1605693846108672 62-63 0.14879409665563845 64-65 0.14319109704335403 66-67 0.13165550960629788 68-69 0.1352809799436584 70-71 0.1359701189334046 72-73 0.11191017942183037 74-75 0.11451692255695733 76-77 0.1359701189334046 78-79 0.14226225753543523 80-81 0.15128098953167912 82-83 0.15340833163046091 84-85 0.14112368007411538 86-87 0.16785028785035977 88-89 0.16296638979259312 90-91 0.15029222489421717 92-93 0.1511012141430497 94-95 0.1724645228251822 96-97 0.16140833642447125 98-99 0.15454690909178073 100-101 0.135730418415232 102-103 0.17456190235919244 104-105 0.13150569678244003 106-107 0.1387866000219326 108-109 0.14894390947949632 110-111 0.1255431463928967 112-113 0.11529594924101828 114-115 0.1132584948365512 116-117 0.11754314159888636 118-119 0.10259182177787071 120-121 0.09477159237248982 122-123 0.12200756374985094 124-125 0.11832216828294728 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1668749.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.91594613084517 #Duplication Level Percentage of deduplicated Percentage of total 1 82.09195092105863 52.469847128463684 2 9.94751524621302 12.71609697225425 3 3.456684494029547 6.628117797351611 4 1.565621034489976 4.002725988071176 5 0.8413187074112267 2.688684059088413 6 0.5225200356666073 2.0038417471172503 7 0.3391489217394285 1.5173916948562076 8 0.2286741995100538 1.1692742253918995 9 0.16531569483992206 0.9509678141374527 >10 0.7711608794801779 8.60766319504123 >50 0.04605069400299839 2.000539338771461 >100 0.021303201769086264 2.4553278099663367 >500 0.001270271687922644 0.5143113301207387 >1k 0.0013679848946859243 1.7826336728155732 >5k 9.771320676328032E-5 0.4925772265527949 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7887 0.4726294967068145 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4574 0.27409754253036256 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4304 0.2579177575537124 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2775 0.16629223448223787 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2431 0.1456779899193947 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 5.992512954314879E-5 0.0 0.0 0.0 0.0 7 5.992512954314879E-5 0.0 0.0 0.0 0.0 8 5.992512954314879E-5 0.0 0.0 0.0 0.0 9 5.992512954314879E-5 0.0 0.0 0.0 0.0 10-11 1.7977538862944638E-4 0.0 0.0 0.0 0.0 12-13 1.7977538862944638E-4 0.0 0.0 0.0 0.0 14-15 1.7977538862944638E-4 0.0 0.0 0.0 0.0 16-17 2.0973795340102077E-4 0.0 0.0 2.9962564771574395E-5 0.0 18-19 2.6966308294416955E-4 0.0 0.0 5.992512954314879E-5 0.0 20-21 2.99625647715744E-4 0.0 0.0 8.988769431472319E-5 0.0 22-23 3.5955077725889276E-4 0.0 0.0 3.2958821248731834E-4 0.0 24-25 4.1947590680204154E-4 0.0 0.0 6.591764249746367E-4 0.0 26-27 4.1947590680204154E-4 0.0 0.0 0.0010486897670051037 0.0 28-29 6.292138602030623E-4 0.0 0.0 0.0017977538862944636 0.0 30-31 8.089892488325086E-4 0.0 0.0 0.004254684197563564 0.0 32-33 8.389518136040831E-4 0.0 0.0 0.009558058162132232 0.0 34-35 8.988769431472318E-4 0.0 0.0 0.016479410624365916 0.0 36-37 9.588020726903807E-4 0.0 0.0 0.027565559589848444 0.0 38-39 9.588020726903807E-4 0.0 0.0 0.04296631788243768 0.0 40-41 9.588020726903807E-4 0.0 5.992512954314879E-5 0.06663674405198146 2.9962564771574395E-5 42-43 9.88764637461955E-4 0.0 5.992512954314879E-5 0.08575286037624592 5.992512954314879E-5 44-45 0.0010187272022335295 0.0 5.992512954314879E-5 0.1082547465196983 5.992512954314879E-5 46-47 0.0011086148965482527 0.0 5.992512954314879E-5 0.13507124199025738 5.992512954314879E-5 48-49 0.001138577461319827 0.0 5.992512954314879E-5 0.1650937318913749 5.992512954314879E-5 50-51 0.001138577461319827 0.0 5.992512954314879E-5 0.2011386973115789 5.992512954314879E-5 52-53 0.001138577461319827 0.0 5.992512954314879E-5 0.2364945237420367 5.992512954314879E-5 54-55 0.001138577461319827 0.0 5.992512954314879E-5 0.2749664569087382 5.992512954314879E-5 56-57 0.0012584277204061246 0.0 5.992512954314879E-5 0.3208990687035618 5.992512954314879E-5 58-59 0.0012584277204061246 0.0 5.992512954314879E-5 0.375371011458284 8.988769431472319E-5 60-61 0.0012584277204061246 0.0 5.992512954314879E-5 0.4430864078420421 1.1985025908629758E-4 62-63 0.0012584277204061246 0.0 5.992512954314879E-5 0.5077156600543281 1.1985025908629758E-4 64-65 0.0012584277204061246 0.0 5.992512954314879E-5 0.5725846127847867 1.1985025908629758E-4 66-67 0.0012584277204061246 0.0 5.992512954314879E-5 0.6439254795059053 1.1985025908629758E-4 68-69 0.0012584277204061246 0.0 5.992512954314879E-5 0.7283899495969736 1.1985025908629758E-4 70-71 0.0012584277204061246 0.0 5.992512954314879E-5 0.8311915093282454 1.1985025908629758E-4 72-73 0.001288390285177699 0.0 5.992512954314879E-5 0.9387271542934258 1.1985025908629758E-4 74-75 0.001378277979492422 0.0 5.992512954314879E-5 1.0405099868224639 1.1985025908629758E-4 76-77 0.001378277979492422 0.0 5.992512954314879E-5 1.1503527492750558 1.1985025908629758E-4 78-79 0.001378277979492422 0.0 5.992512954314879E-5 1.2804202429484604 1.1985025908629758E-4 80-81 0.001737828756751315 0.0 5.992512954314879E-5 1.443746183518312 1.1985025908629758E-4 82-83 0.001737828756751315 0.0 5.992512954314879E-5 1.6164803694264385 1.1985025908629758E-4 84-85 0.0017677913215228894 0.0 5.992512954314879E-5 1.7942183036514179 1.1985025908629758E-4 86-87 0.0018576790158376126 0.0 5.992512954314879E-5 1.9785480021261437 1.1985025908629758E-4 88-89 0.0019176041453807613 0.0 5.992512954314879E-5 2.181064977417215 1.1985025908629758E-4 90-91 0.0020074918396954843 0.0 5.992512954314879E-5 2.4131550041378302 1.1985025908629758E-4 92-93 0.0020973795340102075 0.0 5.992512954314879E-5 2.6697544088415937 1.1985025908629758E-4 94-95 0.0020973795340102075 0.0 5.992512954314879E-5 2.9241365837522597 1.1985025908629758E-4 96-97 0.0022172297930965054 0.0 5.992512954314879E-5 3.1738745611233323 1.1985025908629758E-4 98-99 0.002277154922639654 0.0 5.992512954314879E-5 3.4590582526191778 1.1985025908629758E-4 100-101 0.002337080052182803 0.0 5.992512954314879E-5 3.801021004357156 1.1985025908629758E-4 102-103 0.002397005181725952 0.0 5.992512954314879E-5 4.175553064001836 1.1985025908629758E-4 104-105 0.002966293912385865 5.992512954314879E-5 5.992512954314879E-5 4.543912835303572 1.1985025908629758E-4 106-107 0.003116106736243737 5.992512954314879E-5 5.992512954314879E-5 4.908497323444089 1.1985025908629758E-4 108-109 0.003116106736243737 5.992512954314879E-5 5.992512954314879E-5 5.300497558350597 1.1985025908629758E-4 110-111 0.003176031865786886 5.992512954314879E-5 5.992512954314879E-5 5.764228173320253 1.1985025908629758E-4 112-113 0.003176031865786886 5.992512954314879E-5 5.992512954314879E-5 6.276348330396003 1.1985025908629758E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 14580 0.0 33.055626 1 TACATGG 14440 0.0 32.302483 2 GTTATCA 1200 0.0 32.269073 1 CGCATAG 260 1.8189894E-12 29.746592 8 ACATGGG 15020 0.0 29.50831 3 ACGCATA 265 1.8189894E-12 29.191471 7 CATGGGG 4045 0.0 27.98185 4 TACCTGG 1830 0.0 27.018944 2 GTGTACG 200 6.046321E-8 26.808153 1 AACGCAT 290 5.456968E-12 26.679773 6 ATGGGAT 3530 0.0 25.99043 5 ATGGGAG 3405 0.0 25.719805 5 CATGGGA 10800 0.0 25.373209 4 GTATAGA 645 0.0 24.937817 1 CCTATAC 425 0.0 23.828756 3 GAGTACT 3780 0.0 23.450312 12-13 AGTACTT 4100 0.0 22.925953 12-13 TTATCAA 1685 0.0 22.273167 2 CGCAGAT 770 0.0 21.633884 8 ACCTGGG 2290 0.0 21.591557 3 >>END_MODULE