FastQCFastQC Report
Thu 26 May 2016
SRR1364593_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364593_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1668749
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107780.6458730462160577No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88400.5297381451614352No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61640.36937849850396914No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA55030.3297679878759478No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA54710.327850383730567No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT31670.1897828852631522No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24100.1444195621989886No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23690.1419626318877195No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG21940.13147573421766845No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA20460.12260681504528244No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT19100.11445699742741418No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA18700.11205999224568823No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCACGC2052.4283509E-929.0233233
GTACATG144050.028.5818961
TACATGG142200.027.9757392
GAGTACT106850.027.75117112-13
AGTACTT109200.026.33607312-13
ACATGGG146200.025.9641633
TACCTGG17750.025.16332
GTACTTT118500.025.04801414-15
GTATCAA230700.024.8200131
ATGGGAG33900.024.0693655
CATGGGG36450.023.9964394
GTACACG3002.2009772E-1023.833411
TCACGCA2752.1173037E-923.800554
TATCACG2302.3447319E-723.3034042
ATGGGAT32550.023.237885
CATGGGA109900.023.1725124
ACTTTTT136750.022.4481216-17
TACTTTT133600.021.94958514-15
TATCAAC261850.021.6515522
ATCAACG266700.021.2603743
Produced by FastQC (version 0.11.5)