##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364593_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1668749 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.51519731247779 31.0 30.0 33.0 26.0 34.0 2 29.607202161619274 31.0 30.0 34.0 26.0 34.0 3 29.56972977961335 31.0 30.0 34.0 25.0 34.0 4 33.22298739954301 35.0 33.0 37.0 27.0 37.0 5 32.63804922130291 35.0 33.0 37.0 25.0 37.0 6 32.61333430012542 35.0 33.0 37.0 25.0 37.0 7 32.418054482729275 35.0 33.0 37.0 23.0 37.0 8 32.340026570802436 35.0 33.0 37.0 23.0 37.0 9 33.586605894595294 38.0 33.0 39.0 21.0 39.0 10-11 33.372631534161215 37.0 33.0 39.0 18.5 39.0 12-13 33.240773177991414 37.0 33.0 39.0 17.0 39.0 14-15 34.158637398434394 38.0 33.0 41.0 17.0 41.0 16-17 34.09771631323824 38.0 33.0 41.0 17.0 41.0 18-19 34.196328207537505 38.5 33.0 41.0 17.5 41.0 20-21 34.236358194072324 39.0 33.0 41.0 18.0 41.0 22-23 34.23194680566101 39.0 33.0 41.0 18.0 41.0 24-25 34.218100804854416 39.0 33.0 41.0 18.0 41.0 26-27 34.08684559511346 39.0 33.0 41.0 18.0 41.0 28-29 33.8318461913685 38.0 33.0 41.0 17.0 41.0 30-31 33.73071714200279 38.0 33.0 40.5 16.5 41.0 32-33 33.48461871737452 38.0 32.5 40.0 16.0 41.0 34-35 33.310306403179865 38.0 32.0 40.0 14.5 41.0 36-37 33.09008364948833 38.0 32.0 40.0 9.0 41.0 38-39 32.917329388661805 38.0 32.0 40.0 9.0 41.0 40-41 32.70896791548639 38.0 31.0 40.0 7.5 41.0 42-43 32.503599103280365 38.0 31.0 40.0 2.0 41.0 44-45 32.23227339761702 38.0 31.0 40.0 2.0 41.0 46-47 31.863121116477075 38.0 30.0 40.0 2.0 41.0 48-49 31.500189663035005 37.5 30.0 40.0 2.0 41.0 50-51 31.272624283220544 37.0 30.0 40.0 2.0 41.0 52-53 31.089991364788833 37.0 29.0 40.0 2.0 41.0 54-55 30.87484546807219 37.0 29.0 40.0 2.0 41.0 56-57 30.67230751898578 36.5 28.0 40.0 2.0 41.0 58-59 30.479723433542134 36.0 28.0 40.0 2.0 41.0 60-61 30.263040457252707 36.0 28.0 40.0 2.0 41.0 62-63 29.987941266182034 35.5 28.0 39.0 2.0 41.0 64-65 29.720517585328892 35.0 28.0 39.0 2.0 41.0 66-67 29.452496450934206 35.0 28.0 39.0 2.0 41.0 68-69 29.16787665490736 35.0 27.0 38.5 2.0 40.0 70-71 28.913519348925455 35.0 27.0 38.0 2.0 40.0 72-73 28.74190756069367 35.0 27.0 37.0 2.0 40.0 74-75 28.453720721330768 34.0 26.0 37.0 2.0 39.0 76-77 28.140217312489774 34.0 26.0 36.0 2.0 39.0 78-79 27.833114506735285 34.0 26.0 36.0 2.0 39.0 80-81 27.579532032678372 34.0 26.0 35.5 2.0 37.0 82-83 27.331164543019952 34.0 26.0 35.0 2.0 37.0 84-85 27.294023247354758 34.0 26.0 35.0 2.0 36.5 86-87 27.125269288550882 34.0 26.0 35.0 2.0 36.0 88-89 26.95760207197128 34.0 26.0 35.0 2.0 36.0 90-91 26.78723013466974 34.0 25.0 35.0 2.0 36.0 92-93 26.66115245612132 34.0 25.0 35.0 2.0 35.0 94-95 26.5899497168238 34.0 25.0 35.0 2.0 35.0 96-97 26.495030409006986 34.0 25.0 35.0 2.0 35.0 98-99 26.388933866027784 34.0 25.0 35.0 2.0 35.0 100-101 26.283884814312998 34.0 24.0 35.0 2.0 35.0 102-103 25.130109740889733 31.5 21.5 34.0 2.0 35.0 104-105 26.01079910759497 33.5 23.5 35.0 2.0 35.0 106-107 26.17753299028194 34.0 24.0 35.0 2.0 35.0 108-109 26.181920109015795 34.0 24.0 35.0 2.0 35.0 110-111 26.116843216085822 34.0 24.0 35.0 2.0 35.0 112-113 26.049393887277233 34.0 23.5 35.0 2.0 35.0 114-115 25.94346693241464 34.0 23.0 35.0 2.0 35.0 116-117 25.823617572205286 34.0 21.5 35.0 2.0 35.0 118-119 25.745123442770605 34.0 20.0 35.0 2.0 35.0 120-121 25.602215491964337 34.0 19.0 35.0 2.0 35.0 122-123 25.40528503687493 34.0 16.5 35.0 2.0 35.0 124-125 24.6034403616122 33.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8553211628933859 1101 2 0.861342298113442 1101 3 0.7344699245685575 1101 4 0.7793197531797489 1101 5 1.009393786040242 1101 6 0.9493662980815287 1101 7 1.023111120102925 1101 8 0.9588280320219056 1101 9 1.0461348280627973 1101 10-11 1.1589131558975438 1101 12-13 1.1757622809401909 1101 14-15 1.2981523443689298 1101 16-17 1.470918088305389 1101 18-19 1.4192742290558797 1101 20-21 1.3422001843822358 1101 22-23 1.230094666460083 1101 24-25 1.2477536304211512 1101 26-27 1.34285762137052 1101 28-29 1.4665758819735117 1101 30-31 1.409450995545619 1101 32-33 1.5323866296441153 1101 34-35 1.4729714716661135 1101 36-37 1.3930373062735484 1101 38-39 1.591310049033666 1101 40-41 1.656294774469643 1101 42-43 1.565177461511496 1101 44-45 1.625108604267119 1101 46-47 1.6515894129159783 1101 48-49 1.6957218818952846 1101 50-51 1.7004900878273688 1101 52-53 1.5766960923683584 1101 54-55 1.5985490466250951 1101 56-57 1.6024612804988898 1101 58-59 1.5085063972243447 1101 60-61 1.5659124012301042 1101 62-63 1.4664825775238093 1101 64-65 1.459048348494342 1101 66-67 1.422119437723353 1101 68-69 1.483406229501803 1101 70-71 1.436970763165263 1101 72-73 1.4750184492887932 1101 74-75 1.5050808648750014 1101 76-77 1.5415639497600289 1101 78-79 1.5919272498299115 1101 80-81 1.5296731165124626 1101 82-83 1.5064828648065323 1101 84-85 1.5944704308050675 1101 86-87 1.6558028627813002 1101 88-89 1.5733144483545267 1101 90-91 1.648885531478836 1101 92-93 1.572019726448051 1101 94-95 1.5027183891932907 1101 96-97 1.5538473440001255 1101 98-99 1.6508352157303072 1101 100-101 1.5821640910208323 1101 102-103 1.5788719633254864 1101 104-105 1.647403028798312 1101 106-107 1.627419684505341 1101 108-109 1.7256437998201193 1101 110-111 1.6718754957978774 1101 112-113 1.5765452863579092 1101 114-115 1.589064473464525 1101 116-117 1.6063429237725124 1101 118-119 1.5257058832633383 1101 120-121 1.536795708815891 1101 122-123 1.534229469079758 1101 124-125 1.5290706047496343 1102 1 0.4548987363753305 1102 2 -0.5209512367370728 1102 3 0.10554868914347182 1102 4 0.5162961675348896 1102 5 0.7079280074176282 1102 6 0.601757370814255 1102 7 0.37054625391238716 1102 8 0.3573755260943656 1102 9 0.7601424164962296 1102 10-11 0.8218650558477023 1102 12-13 0.9982575607558601 1102 14-15 0.9289860470270526 1102 16-17 1.075407790960881 1102 18-19 1.0346256942924228 1102 20-21 0.9387335798300285 1102 22-23 0.9506269836656642 1102 24-25 0.8481733136965346 1102 26-27 0.8851133323253961 1102 28-29 1.001373422451131 1102 30-31 0.9616411052194778 1102 32-33 0.9878688933482564 1102 34-35 0.937182010777363 1102 36-37 0.8425411078822407 1102 38-39 0.7813800526442307 1102 40-41 0.7201276495555149 1102 42-43 0.6769291188735878 1102 44-45 0.505553982034634 1102 46-47 0.5325833570845866 1102 48-49 0.6391988450632233 1102 50-51 0.5476122948531597 1102 52-53 0.7012672348352993 1102 54-55 0.8326430028508298 1102 56-57 0.6741514925104788 1102 58-59 0.7446754440510048 1102 60-61 0.6857481647698123 1102 62-63 0.7751323666226533 1102 64-65 0.6779864638964881 1102 66-67 0.722901212604647 1102 68-69 0.5564812041309182 1102 70-71 0.4256822374430058 1102 72-73 0.4819866142040041 1102 74-75 0.5318099044476732 1102 76-77 0.6557515080539353 1102 78-79 0.7364223009872788 1102 80-81 0.6713934680549052 1102 82-83 0.5811721638275422 1102 84-85 0.7766187873398813 1102 86-87 0.6321216150954001 1102 88-89 0.72898016435893 1102 90-91 0.7098428476084742 1102 92-93 0.8441095648529888 1102 94-95 0.663132214742113 1102 96-97 0.636481615872885 1102 98-99 0.6861155340647294 1102 100-101 0.5454838154612851 1102 102-103 0.4534254471943946 1102 104-105 0.4375027481333724 1102 106-107 0.7397316532895388 1102 108-109 0.7705573351108725 1102 110-111 0.6737799961285447 1102 112-113 0.6888576658071806 1102 114-115 0.7242103236282809 1102 116-117 0.7853313768563233 1102 118-119 0.6706473273522775 1102 120-121 0.7635753916050483 1102 122-123 0.786065552609692 1102 124-125 0.6660935228173557 1103 1 0.2657924895473194 1103 2 -0.9442084335610268 1103 3 0.06571875059341536 1103 4 0.25410896926560156 1103 5 0.5280834702985189 1103 6 0.5651388534665784 1103 7 0.43269642778187745 1103 8 0.5487979428175151 1103 9 0.7195960789089426 1103 10-11 0.861566959931416 1103 12-13 0.7819888678887708 1103 14-15 0.8866833603491671 1103 16-17 1.0554948016002896 1103 18-19 1.0253563949115474 1103 20-21 1.035028037983544 1103 22-23 0.9916456958369295 1103 24-25 0.8144256722695928 1103 26-27 0.9544091823587522 1103 28-29 1.1015227739961446 1103 30-31 1.0028870125713567 1103 32-33 0.922705454712073 1103 34-35 0.9128701465361431 1103 36-37 1.0751983774350506 1103 38-39 0.9901966775897009 1103 40-41 1.045875545731441 1103 42-43 0.934325749191224 1103 44-45 1.0035450288615877 1103 46-47 1.0092805892302117 1103 48-49 0.9655288360810097 1103 50-51 1.0180011896451262 1103 52-53 1.06022588564241 1103 54-55 1.1135989649341482 1103 56-57 1.0819844959680687 1103 58-59 1.0543330706483864 1103 60-61 1.0493092916579094 1103 62-63 1.0567874034919598 1103 64-65 1.01290235383172 1103 66-67 0.9111452066992207 1103 68-69 0.8097403082724384 1103 70-71 0.9475436275937241 1103 72-73 0.911015096592994 1103 74-75 0.7627360688188496 1103 76-77 0.7512997034609299 1103 78-79 0.8693149823655908 1103 80-81 0.8726661240507134 1103 82-83 0.9095878297024633 1103 84-85 0.8856082918134973 1103 86-87 0.8865691628723837 1103 88-89 0.796098603499992 1103 90-91 0.9094787743437216 1103 92-93 0.8896929697382312 1103 94-95 0.8667977623171481 1103 96-97 0.8930286979080009 1103 98-99 0.9606184600482628 1103 100-101 1.0363400756537189 1103 102-103 0.9629160020126157 1103 104-105 0.9779687341934569 1103 106-107 0.9570178571798067 1103 108-109 0.9551049939610579 1103 110-111 0.9336435998235935 1103 112-113 0.8415615359459103 1103 114-115 0.8364214788621496 1103 116-117 0.818283092683167 1103 118-119 0.9283678386717753 1103 120-121 0.8986715527489615 1103 122-123 0.847381357540602 1103 124-125 0.7657838642007988 1104 1 0.35931220314088463 1104 2 -0.5820849384713398 1104 3 0.08075933276967007 1104 4 0.26806948227112315 1104 5 0.6363354045516019 1104 6 0.5524455487378717 1104 7 0.789547230325681 1104 8 0.6136377845402734 1104 9 0.6775161467163215 1104 10-11 0.7456987534507391 1104 12-13 0.7929450265338573 1104 14-15 0.8109261061364492 1104 16-17 0.7878358796630138 1104 18-19 0.6785540298848147 1104 20-21 0.8159720679566718 1104 22-23 0.8275774331502177 1104 24-25 0.8202507788777922 1104 26-27 0.722187397437537 1104 28-29 1.0449898828513042 1104 30-31 0.9432090911916475 1104 32-33 0.8545774952936043 1104 34-35 0.7317120963069712 1104 36-37 0.8104393077800793 1104 38-39 0.8769665858786766 1104 40-41 0.8824960321259141 1104 42-43 0.7789877508851291 1104 44-45 0.8079817690912918 1104 46-47 0.7617703110488954 1104 48-49 0.7703639703870842 1104 50-51 0.7018285444949584 1104 52-53 0.5332662787502898 1104 54-55 0.679608908387273 1104 56-57 0.833422926880619 1104 58-59 0.769420373292192 1104 60-61 0.7368075633543079 1104 62-63 0.7770345364372524 1104 64-65 0.7559778853614461 1104 66-67 0.874100023859171 1104 68-69 0.9166475419384383 1104 70-71 0.8502611960972501 1104 72-73 0.7474754536492618 1104 74-75 0.8292159500787655 1104 76-77 0.7865310517692485 1104 78-79 0.7976379716009134 1104 80-81 0.7386004079992539 1104 82-83 0.7487401602917316 1104 84-85 0.7097111977429762 1104 86-87 0.7690422672529209 1104 88-89 0.6708834096052279 1104 90-91 0.6573088288841582 1104 92-93 0.6685541929077239 1104 94-95 0.6579030920517503 1104 96-97 0.7015101508791695 1104 98-99 0.7503517578830454 1104 100-101 0.6700607740748694 1104 102-103 0.7834113354321239 1104 104-105 0.7700891810379105 1104 106-107 0.7196402612677986 1104 108-109 0.6769844493669623 1104 110-111 0.5614809920176427 1104 112-113 0.662343848149245 1104 114-115 0.7907341349319879 1104 116-117 0.8072034608134437 1104 118-119 0.7245072746692358 1104 120-121 0.7154667087400206 1104 122-123 0.6986986946604468 1104 124-125 0.7465386007173009 1105 1 0.5985706215130655 1105 2 0.35908222107735455 1105 3 0.49619736710706874 1105 4 0.5005586561088933 1105 5 0.693202775015898 1105 6 0.5898424481635303 1105 7 0.6736637225963023 1105 8 0.659440862249113 1105 9 0.6341970515066748 1105 10-11 0.8561502290366931 1105 12-13 0.783771098672652 1105 14-15 0.7149386908607482 1105 16-17 0.8552206038308228 1105 18-19 0.9002748265859779 1105 20-21 0.8925438459015922 1105 22-23 0.7639106829126305 1105 24-25 0.7845960535325389 1105 26-27 0.7572622524388848 1105 28-29 0.9174176305093766 1105 30-31 0.8679561959563387 1105 32-33 0.7695601613177985 1105 34-35 0.8370248923196755 1105 36-37 0.8194131625089653 1105 38-39 0.877991883013685 1105 40-41 0.8418463909371923 1105 42-43 0.9105683783380769 1105 44-45 0.8149024665014863 1105 46-47 0.9767054752377895 1105 48-49 1.0010341563525778 1105 50-51 1.0163478579210121 1105 52-53 0.9930765106269774 1105 54-55 0.8821242377483465 1105 56-57 0.9589182554525841 1105 58-59 0.7479400837577863 1105 60-61 0.8886140621894434 1105 62-63 0.8601923397709577 1105 64-65 0.8461641894856555 1105 66-67 0.8043201695459636 1105 68-69 0.7740554360661989 1105 70-71 0.8937917816560059 1105 72-73 0.7560323857949562 1105 74-75 0.7789972831933873 1105 76-77 0.8866462957575045 1105 78-79 0.8761717244664808 1105 80-81 0.6697385911177456 1105 82-83 0.7431878201972317 1105 84-85 0.5490186091787734 1105 86-87 0.6060709730873199 1105 88-89 0.6528661871608747 1105 90-91 0.6405844703220431 1105 92-93 0.40845360645112194 1105 94-95 0.561768650984316 1105 96-97 0.6159054904178767 1105 98-99 0.7228344013834835 1105 100-101 0.8483304397259452 1105 102-103 0.3758459769144302 1105 104-105 0.41118205877614145 1105 106-107 0.7034730939522902 1105 108-109 0.6568002081085922 1105 110-111 0.5729842651084596 1105 112-113 0.41442077191920745 1105 114-115 0.6161060177599644 1105 116-117 0.343733739117031 1105 118-119 0.16394779376581425 1105 120-121 0.1723688123971634 1105 122-123 0.2001954374139352 1105 124-125 0.11803091327999482 1106 1 0.49130696069173396 1106 2 0.34640336629121293 1106 3 0.6147844095280526 1106 4 0.44501202717513166 1106 5 0.6482156530030139 1106 6 0.6753352228642981 1106 7 0.6451101298293977 1106 8 0.6770612507143312 1106 9 0.7419183352128726 1106 10-11 0.9827748169815393 1106 12-13 0.7874278854763901 1106 14-15 0.8559063763372521 1106 16-17 1.0619204988934214 1106 18-19 1.0561727829389085 1106 20-21 1.1065396139198995 1106 22-23 0.9758814048138689 1106 24-25 1.0380475661829323 1106 26-27 1.0565721864261306 1106 28-29 1.2753384661754552 1106 30-31 1.1374162696185195 1106 32-33 1.0130365937392298 1106 34-35 0.9200427039057004 1106 36-37 1.0987295236639056 1106 38-39 1.1643868263647335 1106 40-41 1.267231353409521 1106 42-43 1.2424579641138322 1106 44-45 1.184977698849245 1106 46-47 0.9846188873115658 1106 48-49 0.9372001127805909 1106 50-51 0.9348788533644665 1106 52-53 0.8852497096734027 1106 54-55 0.8249399933426318 1106 56-57 0.784503052179673 1106 58-59 0.8420626128574291 1106 60-61 0.9062331536241395 1106 62-63 1.053844184125463 1106 64-65 0.7791021421397701 1106 66-67 0.7478180106005183 1106 68-69 0.9171312539087921 1106 70-71 0.8596353222380344 1106 72-73 0.8416750056684243 1106 74-75 0.7549276775166014 1106 76-77 0.751252560781122 1106 78-79 0.6955323769247101 1106 80-81 0.6487782727745746 1106 82-83 0.6679581427961878 1106 84-85 0.5182332846670761 1106 86-87 0.62900659261242 1106 88-89 0.5702423676684631 1106 90-91 0.8435266390882923 1106 92-93 0.8626723965780023 1106 94-95 0.8455614459340168 1106 96-97 0.7032479832281346 1106 98-99 0.6370558221934353 1106 100-101 0.794246937511609 1106 102-103 0.8472563724766928 1106 104-105 0.8145045321492468 1106 106-107 0.6776194609469215 1106 108-109 0.6163163760517634 1106 110-111 0.577030389955123 1106 112-113 0.6288342600599997 1106 114-115 0.46589012321556567 1106 116-117 0.6593584859099728 1106 118-119 0.775808283332271 1106 120-121 0.6928021548707441 1106 122-123 0.7825687011287705 1106 124-125 0.8051009381265217 1107 1 0.5231486764660893 1107 2 0.547323744782986 1107 3 0.598910808497898 1107 4 0.38569655526497115 1107 5 0.5647509410559337 1107 6 0.5226499107505802 1107 7 0.36845164929437146 1107 8 0.3130315925232594 1107 9 0.4407377178365479 1107 10-11 0.5891309628609562 1107 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-2.15026847887842 2216 122-123 -2.143851063001776 2216 124-125 -2.2803239598671468 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 70015.0 3 22389.0 4 10184.0 5 8174.0 6 9179.0 7 11010.0 8 12247.0 9 12813.0 10 13874.0 11 15834.0 12 18357.0 13 21354.0 14 25177.0 15 20435.0 16 12330.0 17 10200.0 18 9198.0 19 8382.0 20 8197.0 21 8449.0 22 9730.0 23 12649.0 24 17070.0 25 23208.0 26 27176.0 27 28824.0 28 32763.0 29 38541.0 30 44006.0 31 52270.0 32 63197.0 33 78098.0 34 100803.0 35 138838.0 36 212837.0 37 325939.0 38 134297.0 39 705.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.199829839690764 19.38538432066884 13.518151332352824 22.896634507287576 2 15.15348692720893 21.393715392951364 39.3900703520481 24.06272732779161 3 19.59941249402996 27.202892705853305 29.03630204422594 24.16139275589079 4 12.099659057334582 17.354934559264635 36.82983850620487 33.715567877195916 5 12.508197291267292 40.007876592852085 32.95275123274073 14.531174883139894 6 30.105174973018613 37.63251740981086 17.481421644914764 14.780885972255764 7 27.346250002996257 34.81999678801113 20.026990294520203 17.806762914472408 8 26.388330419973283 35.59468799681677 18.85208620349735 19.164895379712586 9 27.340170847977657 16.50363898402083 19.652910737774423 36.50327943022709 10-11 24.419383783968527 28.078179587120687 26.162574039491528 21.33986258941926 12-13 26.5850436187583 25.32638781305707 27.349514310003176 20.739054258181458 14-15 23.417779551681733 25.711778961978528 26.98030007742851 23.89014140891123 16-17 21.06080455187258 29.380173093468677 27.534722118131643 22.024300236527097 18-19 20.946243750170414 27.21730185939078 31.305582546889266 20.53087184354954 20-21 22.188641025295272 26.04923011460258 31.08209463964389 20.68003422045826 22-23 21.516774404664158 26.10412968354635 30.719772929135047 21.65932298265444 24-25 21.357855495344115 26.106053097260286 31.379913935528954 21.15617747186665 26-27 21.65271709526118 26.008315210975407 31.312737865311078 21.02622982845233 28-29 20.982641648352406 25.845426341297877 31.96523233354608 21.206699676803638 30-31 20.998933931945427 26.36190343784476 31.700902891926823 20.938259738282987 32-33 20.930045808333713 26.29910133206128 31.310960920289666 21.459891939315344 34-35 21.311173819430003 25.908509907721296 31.313606779689458 21.466709493159247 36-37 21.751279628918848 25.747335348619664 31.335545174503842 21.165839847957642 38-39 21.902994398798143 25.516659485638645 31.485711751737377 21.094634363825836 40-41 21.49891925028869 25.729693321164536 31.646880387643677 21.124507040903097 42-43 21.370392998367286 26.115923416870597 31.059992035699725 21.4536915490624 44-45 21.40223005377082 26.568735022462935 30.303089320203334 21.725945603562906 46-47 21.469305571993367 26.5749311983988 29.45532502670416 22.500438202903673 48-49 21.965548960446323 26.617487992473386 29.277151392213906 22.13981165486638 50-51 22.34375777158681 26.936207519570164 28.863141569772143 21.85689313907088 52-53 22.003795059591063 27.121672319106203 28.95888745368161 21.915645167621125 54-55 21.486934224380057 27.20496012282255 29.111957520274167 22.196148132523223 56-57 21.90446027627771 27.560054281141916 28.601541481357412 21.933943961222962 58-59 21.890739709806567 26.874443070827308 28.944167157553352 22.290650061812773 60-61 21.89933896589601 26.72127443971502 29.230010025474172 22.149376568914796 62-63 22.369871083068812 26.559386702254205 29.081245891383308 21.98949632329368 64-65 22.11132005144007 26.539756390757567 29.328300787780947 22.02062277002142 66-67 21.751024270276716 26.966458107240815 29.269440760713568 22.01307686176891 68-69 21.743370815738956 27.09465862985723 29.099766080784978 22.062204473618845 70-71 21.539398675295086 27.182308423855233 28.74854157215974 22.529751328689933 72-73 21.901349811486625 26.89762089320851 28.500741139362066 22.700288155942793 74-75 21.843122449346495 27.05718258034746 28.623710815070503 22.475984155235544 76-77 22.122149865437155 26.80395412818197 28.937873373695194 22.136022632685677 78-79 21.947349181713037 26.789564519125324 29.127150587572586 22.135935711589052 80-81 21.438131750091014 27.597855251980924 28.836913156881515 22.127099841046547 82-83 21.6534646256512 27.4904276838066 28.34466270900069 22.511444981541512 84-85 21.75720571015601 26.87167489839676 28.44713934894825 22.92398004249898 86-87 21.963388011215628 26.78024716807801 28.762218028779746 22.494146791926617 88-89 22.344372940448203 26.298262950269937 28.96331323644239 22.394050872839475 90-91 21.735803287372757 26.91710976306203 29.20202498997752 22.145061959587693 92-93 21.391554176241677 27.213838232531185 28.997883584882988 22.39672400634415 94-95 21.327476744841075 27.384515258332726 28.576877761943404 22.7111302348828 96-97 21.666936846530547 26.998429560813392 28.229910379191697 23.104723213464364 98-99 21.754767177524684 26.76807521415257 28.24830402309303 23.22885358522971 100-101 21.73532257334781 27.10624664231103 28.51023628841923 22.648194495921935 102-103 21.746748756251698 26.840004410497464 28.74829362886862 22.66495320438221 104-105 21.404089733598326 26.626774280826936 29.013515890287252 22.95562009528749 106-107 21.495129956714056 27.17926421685281 28.470261663323686 22.855344163109446 108-109 21.469226364500148 27.06125661870704 28.23021818997933 23.239298826813485 110-111 21.158255300125724 26.773500150413426 28.597128122277127 23.471116427183723 112-113 21.846818300446788 26.557814910665034 28.344244577263083 23.2511222116251 114-115 21.491484593166103 26.648878794779296 28.46848520440572 23.391151407648884 116-117 20.937269003407103 26.76179144440306 28.79571504035577 23.505224511834065 118-119 20.952199174749527 27.145053456107142 28.543083246264843 23.35966412287848 120-121 20.933167160460606 27.142354994041213 28.25074683399024 23.67373101150794 122-123 20.87725001685406 27.02755825886336 27.943490213409838 24.15170151087274 124-125 20.826691531972934 26.85623865899933 28.269863680273073 24.047206128754663 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 535.0 1 402.5 2 579.5 3 989.0 4 768.0 5 592.0 6 1319.5 7 3027.0 8 4706.0 9 5191.0 10 4098.0 11 2685.5 12 2296.0 13 2397.5 14 2634.0 15 2837.0 16 3086.5 17 3391.0 18 3647.5 19 3987.5 20 4452.0 21 4965.0 22 5570.0 23 6276.0 24 7152.0 25 8395.0 26 10392.0 27 12726.5 28 14749.5 29 17160.0 30 20134.0 31 22950.5 32 25822.0 33 29308.0 34 33470.5 35 36703.0 36 39697.5 37 43310.5 38 46482.0 39 48325.0 40 49250.5 41 51484.0 42 54200.5 43 56043.5 44 57470.5 45 58909.0 46 60083.0 47 63066.0 48 77491.0 49 78471.5 50 66980.0 51 61572.0 52 54004.0 53 50565.0 54 48420.5 55 44299.5 56 38924.5 57 34539.5 58 31438.5 59 28550.0 60 24866.0 61 20318.5 62 16382.0 63 12944.5 64 9877.0 65 7727.0 66 5850.0 67 4392.5 68 3312.0 69 2296.0 70 1643.5 71 1314.5 72 1151.0 73 1056.0 74 859.5 75 667.5 76 490.0 77 315.5 78 202.5 79 113.0 80 80.5 81 55.5 82 36.0 83 30.0 84 20.5 85 13.5 86 8.5 87 5.5 88 3.0 89 2.5 90 2.5 91 0.5 92 1.0 93 1.5 94 1.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0545318678842654 2 0.07658431555614416 3 0.0 4 0.00838951813604083 5 0.030861441714721626 6 3.5955077725889276E-4 7 5.9925129543148784E-5 8 0.0 9 8.389518136040831E-4 10-11 0.05833711361025534 12-13 0.008599256089441851 14-15 0.006771539638375813 16-17 0.05177531192528056 18-19 2.0973795340102077E-4 20-21 0.009378282773502786 22-23 0.011685400260914013 24-25 0.0 26-27 0.0 28-29 0.052584301174113064 30-31 0.0 32-33 0.003415732383959481 34-35 0.0 36-37 2.0973795340102077E-4 38-39 0.0 40-41 0.0 42-43 0.0031460693010153117 44-45 0.0 46-47 8.988769431472319E-5 48-49 2.3970051817259514E-4 50-51 5.99251295431488E-4 52-53 2.9962564771574392E-5 54-55 0.0 56-57 0.0017677913215228894 58-59 0.0 60-61 0.0 62-63 0.0 64-65 6.591764249746367E-4 66-67 0.0 68-69 6.292138602030623E-4 70-71 0.0 72-73 0.0020674169692386332 74-75 0.0035355826430457787 76-77 7.191015545177855E-4 78-79 0.001138577461319827 80-81 0.001288390285177699 82-83 2.6966308294416955E-4 84-85 1.7977538862944638E-4 86-87 3.5955077725889276E-4 88-89 0.0 90-91 0.0 92-93 0.0075206037576651735 94-95 0.005213486270253944 96-97 0.006441951425888496 98-99 0.0012284651556345502 100-101 2.6966308294416955E-4 102-103 1.7977538862944638E-4 104-105 5.692887306599135E-4 106-107 0.004674160104365606 108-109 0.005303373964568668 110-111 8.988769431472318E-4 112-113 0.0034456949487310555 114-115 5.393261658883391E-4 116-117 0.0017078661919797404 118-119 0.002606743135126972 120-121 0.012314614121117076 122-123 6.89138989746211E-4 124-125 0.0014981282385787198 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1668749.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.15371710455336 #Duplication Level Percentage of deduplicated Percentage of total 1 77.6595224362952 42.8321133092614 2 11.86799052350315 13.091275838656259 3 4.291267731894357 7.10038099514399 4 2.0815059892280665 4.59211169925273 5 1.121290478120751 3.092166891115064 6 0.6829986182338478 2.2601947543722285 7 0.4832662864133436 1.8657752432906731 8 0.31455190277389217 1.3878965328232178 9 0.22885135472426088 1.1359802589710108 >10 1.1485816963374804 11.200119767998805 >50 0.07818961132589963 2.9659253056999035 >100 0.037270092741466594 3.795421661392363 >500 0.0028498892701240178 1.0985811769997684 >1k 0.0013153335092880082 1.3599869116105434 >5k 4.384445030960027E-4 1.570489864412282 >10k+ 1.0961112577400068E-4 0.6515797889997527 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10778 0.6458730462160577 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8840 0.5297381451614352 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6164 0.36937849850396914 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 5503 0.3297679878759478 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5471 0.327850383730567 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3167 0.1897828852631522 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2410 0.1444195621989886 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2369 0.1419626318877195 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2194 0.13147573421766845 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2046 0.12260681504528244 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1910 0.11445699742741418 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1870 0.11205999224568823 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 2.9962564771574395E-5 0.0 0.0 2.9962564771574395E-5 0.0 18-19 1.1985025908629758E-4 0.0 0.0 5.992512954314879E-5 0.0 20-21 1.1985025908629758E-4 0.0 0.0 1.1985025908629758E-4 0.0 22-23 1.49812823857872E-4 0.0 0.0 3.2958821248731834E-4 0.0 24-25 3.2958821248731834E-4 0.0 0.0 6.591764249746367E-4 0.0 26-27 3.5955077725889276E-4 0.0 0.0 9.288395079188063E-4 0.0 28-29 4.494384715736159E-4 0.0 0.0 0.0015580533681218685 0.0 30-31 4.794010363451903E-4 0.0 0.0 0.004074908808934118 0.0 32-33 4.794010363451903E-4 0.0 0.0 0.009528095597360658 0.0 34-35 5.093636011167647E-4 0.0 0.0 0.016479410624365916 0.0 36-37 5.393261658883391E-4 0.0 0.0 0.02753559702507687 0.0 38-39 6.591764249746367E-4 0.0 0.0 0.0435356066130976 0.0 40-41 6.891389897462112E-4 0.0 0.0 0.06771539638375813 0.0 42-43 8.389518136040831E-4 0.0 0.0 0.088000052734114 0.0 44-45 8.389518136040831E-4 0.0 0.0 0.11080156452528211 0.0 46-47 9.288395079188063E-4 0.0 0.0 0.1374982097367549 0.0 48-49 9.588020726903807E-4 0.0 0.0 0.16752069963787244 0.0 50-51 9.588020726903807E-4 0.0 0.0 0.2039851409648785 0.0 52-53 9.588020726903807E-4 0.0 0.0 0.2395507053487373 0.0 54-55 9.588020726903807E-4 0.0 0.0 0.27805260108021035 0.0 56-57 9.588020726903807E-4 0.0 0.0 0.32326611132051614 0.0 58-59 9.588020726903807E-4 0.0 0.0 0.3772886156036648 0.0 60-61 9.588020726903807E-4 0.0 0.0 0.4448841617283366 0.0 62-63 9.588020726903807E-4 0.0 0.0 0.5087343872565617 0.0 64-65 9.588020726903807E-4 0.0 0.0 0.5736333025517918 0.0 66-67 0.0010187272022335295 0.0 0.0 0.6456033831331135 0.0 68-69 0.0010187272022335295 0.0 0.0 0.7315360188979889 0.0 70-71 0.0010786523317766782 0.0 0.0 0.8352664181371794 0.0 72-73 0.001138577461319827 0.0 0.0 0.9433713518330198 0.0 74-75 0.001138577461319827 0.0 0.0 1.0464126120824642 0.0 76-77 0.0011685400260914014 0.0 0.0 1.1564351499236853 0.0 78-79 0.001198502590862976 0.0 0.0 1.2865625687266329 0.0 80-81 0.001438203109035571 0.0 0.0 1.4491394451771957 0.0 82-83 0.001438203109035571 0.0 0.0 1.6219335562148651 0.0 84-85 0.0015580533681218687 2.9962564771574395E-5 0.0 1.799222051968271 0.0 86-87 0.001737828756751315 5.992512954314879E-5 0.0 1.9839412637850269 0.0 88-89 0.001737828756751315 5.992512954314879E-5 0.0 2.1867279021590424 0.0 90-91 0.0017977538862944636 5.992512954314879E-5 0.0 2.4203160571182365 0.0 92-93 0.0017977538862944636 5.992512954314879E-5 0.0 2.6778443013299187 0.0 94-95 0.0017977538862944636 5.992512954314879E-5 0.0 2.932915615230331 0.0 96-97 0.0017977538862944636 5.992512954314879E-5 0.0 3.182084303870744 0.0 98-99 0.0017977538862944636 5.992512954314879E-5 0.0 3.469665000548315 0.0 100-101 0.0018876415806091868 5.992512954314879E-5 0.0 3.8137550943850753 0.0 102-103 0.00197752927492391 5.992512954314879E-5 0.0 4.189066180713816 0.0 104-105 0.002277154922639654 5.992512954314879E-5 0.0 4.558204978699613 0.0 106-107 0.0025468180055838235 5.992512954314879E-5 0.0 4.923029167358303 0.0 108-109 0.0025767805703553978 5.992512954314879E-5 0.0 5.318145509001054 0.0 110-111 0.002666668264670121 5.992512954314879E-5 0.0 5.789276877469289 0.0 112-113 0.002816481088527993 5.992512954314879E-5 0.0 6.308258461877729 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCACGC 205 2.4283509E-9 29.023323 3 GTACATG 14405 0.0 28.581896 1 TACATGG 14220 0.0 27.975739 2 GAGTACT 10685 0.0 27.751171 12-13 AGTACTT 10920 0.0 26.336073 12-13 ACATGGG 14620 0.0 25.964163 3 TACCTGG 1775 0.0 25.1633 2 GTACTTT 11850 0.0 25.048014 14-15 GTATCAA 23070 0.0 24.820013 1 ATGGGAG 3390 0.0 24.069365 5 CATGGGG 3645 0.0 23.996439 4 GTACACG 300 2.2009772E-10 23.83341 1 TCACGCA 275 2.1173037E-9 23.80055 4 TATCACG 230 2.3447319E-7 23.303404 2 ATGGGAT 3255 0.0 23.23788 5 CATGGGA 10990 0.0 23.172512 4 ACTTTTT 13675 0.0 22.44812 16-17 TACTTTT 13360 0.0 21.949585 14-15 TATCAAC 26185 0.0 21.651552 2 ATCAACG 26670 0.0 21.260374 3 >>END_MODULE