FastQCFastQC Report
Thu 26 May 2016
SRR1364592_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364592_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2428495
Sequences flagged as poor quality0
Sequence length125
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA85430.3517816590110336No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29620.12196854430418838No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG139450.035.4515571
ACGCATA4300.034.5959977
GTATCAA108700.034.0281071
TACATGG139650.033.566772
CGCATAG4300.033.2101028
ACATGGG144650.030.7182273
AACGCAT5000.029.7568576
ATCAACG125200.028.8775373
TCAACGC125150.028.5083274
CAACGCA126300.028.3908165
TATCAAC133300.027.7930362
CATGGGA101800.027.7336044
AACGCAG125350.027.4423036
CGCAGAT7100.025.1414538
ATGGGAT40150.024.3300515
ACGCAGA143300.023.7522167
CGCAGAG139800.022.8131398
GAGTACT44200.022.54513212-13
GGTATCA77450.022.3793431
CATGGGG42550.022.2573434
Produced by FastQC (version 0.11.5)