FastQCFastQC Report
Thu 26 May 2016
SRR1364592_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364592_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2428495
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126050.5190457464396674No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100910.41552484151707125No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71790.2956151855367213No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28800.11859196745309339No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28510.11739781222526709No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA152500.041.062971
GGTATCA103050.038.2182661
ATCAACG192050.032.0362663
TCAACGC192650.031.9989264
TATCAAC194050.031.822982
CAACGCA194900.030.9553725
AACGCAG197250.030.667046
GTACATG138300.030.6312621
TACATGG140250.028.7874072
ACATGGG142400.026.8262943
ACGCAGA230250.026.0659737
CGCAGAG230150.025.9220778
GAGTACT129750.025.43019712-13
AACGCCG3750.025.4029356
AGTACTT135700.024.2493312-13
GTACTTT137200.024.02762614-15
GCAGAGT243100.023.9048969
CATGGGA106550.023.0085054
AGAGTAC230650.021.05119910-11
TACTTTT160300.020.60228514-15
Produced by FastQC (version 0.11.5)