##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364592_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2428495 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.612824403591524 31.0 30.0 33.0 26.0 34.0 2 29.731342662842625 31.0 30.0 34.0 26.0 34.0 3 29.694131962388226 31.0 30.0 34.0 25.0 34.0 4 33.37208518032774 35.0 33.0 37.0 28.0 37.0 5 32.785326302916005 35.0 33.0 37.0 26.0 37.0 6 32.76427046380577 35.0 33.0 37.0 25.0 37.0 7 32.57836726038143 35.0 33.0 37.0 25.0 37.0 8 32.504710118818444 35.0 33.0 37.0 24.0 37.0 9 33.77407324289323 38.0 33.0 39.0 23.0 39.0 10-11 33.566058814203856 37.0 33.0 39.0 20.5 39.0 12-13 33.43125268942288 37.0 33.0 39.0 19.0 39.0 14-15 34.391007805245636 38.0 33.0 41.0 19.0 41.0 16-17 34.32698399626106 38.0 33.0 41.0 18.5 41.0 18-19 34.401233685883646 38.5 33.0 41.0 19.0 41.0 20-21 34.43884916378251 39.0 33.0 41.0 19.0 41.0 22-23 34.423415119240516 39.0 33.0 41.0 19.0 41.0 24-25 34.41710915608226 39.0 33.0 41.0 20.0 41.0 26-27 34.30981327941791 39.0 33.0 41.0 19.0 41.0 28-29 34.06653750573915 38.5 33.0 41.0 18.0 41.0 30-31 33.97301209185113 38.0 33.0 41.0 18.0 41.0 32-33 33.757966765424676 38.0 33.0 40.5 16.5 41.0 34-35 33.60212909641568 38.0 33.0 40.0 16.0 41.0 36-37 33.41389214307627 38.0 32.5 40.0 15.0 41.0 38-39 33.27054822019399 38.0 32.0 40.0 12.0 41.0 40-41 33.0811924669394 38.0 32.0 40.0 9.0 41.0 42-43 32.87983586542283 38.0 32.0 40.0 8.0 41.0 44-45 32.63446661409638 38.0 31.0 40.0 4.0 41.0 46-47 32.30733684854199 38.0 31.0 40.0 2.0 41.0 48-49 32.00250340231295 38.0 30.0 40.0 2.0 41.0 50-51 31.79592154811931 38.0 30.0 40.0 2.0 41.0 52-53 31.641188884473717 37.5 30.0 40.0 2.0 41.0 54-55 31.438163965748334 37.0 30.0 40.0 2.0 41.0 56-57 31.229540929670435 37.0 29.5 40.0 2.0 41.0 58-59 31.0586987002238 37.0 29.0 40.0 2.0 41.0 60-61 30.859787440369445 36.0 29.0 40.0 2.0 41.0 62-63 30.610982316208187 36.0 28.5 40.0 2.0 41.0 64-65 30.357695609832426 35.5 28.0 39.0 2.0 41.0 66-67 30.09098927525072 35.0 28.0 39.0 2.0 41.0 68-69 29.792515735054018 35.0 28.0 39.0 2.0 40.5 70-71 29.54779688654908 35.0 28.0 38.0 2.0 40.0 72-73 29.39238231909063 35.0 28.0 37.5 2.0 40.0 74-75 29.109733600439778 35.0 28.0 37.0 2.0 39.5 76-77 28.808848690238193 34.5 28.0 37.0 2.0 39.0 78-79 28.49488242718227 34.0 27.5 36.0 2.0 39.0 80-81 28.21232306428467 34.0 27.0 36.0 2.0 38.0 82-83 27.942832906800305 34.0 26.5 35.5 2.0 37.0 84-85 27.89561333253723 34.0 27.0 35.0 2.0 37.0 86-87 27.728840907640325 34.0 27.0 35.0 2.0 36.0 88-89 27.569244737996165 34.0 27.0 35.0 2.0 36.0 90-91 27.398755813785904 34.0 26.0 35.0 2.0 36.0 92-93 27.25332397225442 34.0 26.0 35.0 2.0 36.0 94-95 27.164884836081605 34.0 26.0 35.0 2.0 35.0 96-97 27.07636952104081 34.0 26.0 35.0 2.0 35.0 98-99 26.977747123218286 34.0 26.0 35.0 2.0 35.0 100-101 26.87700098208973 34.0 25.0 35.0 2.0 35.0 102-103 25.722639330120096 31.5 23.5 34.0 2.0 35.0 104-105 26.622870749167696 33.5 25.0 35.0 2.0 35.0 106-107 26.78985338656246 34.0 25.0 35.0 2.0 35.0 108-109 26.799458924148496 34.0 25.0 35.0 2.0 35.0 110-111 26.73780345440283 34.0 25.0 35.0 2.0 35.0 112-113 26.683452920430142 34.0 25.0 35.0 2.0 35.0 114-115 26.602625082612896 34.0 25.0 35.0 2.0 35.0 116-117 26.485997706398408 34.0 25.0 35.0 2.0 35.0 118-119 26.38617332957243 34.0 25.0 35.0 2.0 35.0 120-121 26.24985618665058 34.0 24.5 35.0 2.0 35.0 122-123 26.06527623075197 34.0 24.0 35.0 2.0 35.0 124-125 25.286037237054224 33.0 20.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6133781379899972 1101 2 0.6475156060383576 1101 3 0.41409724042373597 1101 4 0.5032059950285444 1101 5 0.7386444649084964 1101 6 0.7544918177299209 1101 7 0.7259779491068201 1101 8 0.8292527199878705 1101 9 0.8861982936762871 1101 10-11 0.9556519568981514 1101 12-13 0.9288810568527595 1101 14-15 0.9739144007901999 1101 16-17 1.16054652244798 1101 18-19 1.1311084538999125 1101 20-21 1.0850274631443568 1101 22-23 0.9775377344230094 1101 24-25 0.8545397175224423 1101 26-27 0.9636815380785393 1101 28-29 1.0773650433033026 1101 30-31 1.0029126623876081 1101 32-33 1.1574153742545121 1101 34-35 1.049586245057185 1101 36-37 1.0879939058801824 1101 38-39 1.1885446917215248 1101 40-41 1.224487214116536 1101 42-43 1.2573655204918168 1101 44-45 1.1674805692755257 1101 46-47 1.2157374624355413 1101 48-49 1.3547843090790224 1101 50-51 1.2385052910753416 1101 52-53 1.279731703583149 1101 54-55 1.1802505874919937 1101 56-57 1.3324134772123841 1101 58-59 1.329472595149614 1101 60-61 1.3014109486786722 1101 62-63 1.2734250811494299 1101 64-65 1.2446577926887485 1101 66-67 1.201881600691145 1101 68-69 1.1656231003231987 1101 70-71 1.1721848253686709 1101 72-73 1.131355065417452 1101 74-75 1.1592600347561053 1101 76-77 1.1525536504368077 1101 78-79 1.0898533678616786 1101 80-81 1.0903900672703273 1101 82-83 1.0142328621171828 1101 84-85 1.0680760512537546 1101 86-87 1.1382796913381767 1101 88-89 1.1366545365984244 1101 90-91 1.0961231234301145 1101 92-93 1.1346928394125548 1101 94-95 1.0696422401562522 1101 96-97 1.1635044821686087 1101 98-99 1.1323824567158631 1101 100-101 1.1526685626465891 1101 102-103 1.1448460211869467 1101 104-105 1.0956236436777225 1101 106-107 1.1307160458666132 1101 108-109 1.125353830806496 1101 110-111 1.1054662644445763 1101 112-113 1.0353715295418446 1101 114-115 1.067803993222423 1101 116-117 1.1404746456809605 1101 118-119 1.1809038190596013 1101 120-121 1.1037754485764317 1101 122-123 1.1618555001034032 1101 124-125 1.0395647580978498 1102 1 0.46105521199173083 1102 2 -0.475734948350496 1102 3 0.06243052480441236 1102 4 0.3508910238371854 1102 5 0.6985571333668332 1102 6 0.6654469330379058 1102 7 0.5879339015052594 1102 8 0.5725419225001218 1102 9 0.8133622459180714 1102 10-11 1.0308784241316928 1102 12-13 1.095809008330889 1102 14-15 1.254675027188405 1102 16-17 1.3812951676831702 1102 18-19 1.3570421034674212 1102 20-21 1.250687234829158 1102 22-23 1.191862261173192 1102 24-25 1.1924384981117413 1102 26-27 1.1609059835673108 1102 28-29 1.2933132685246918 1102 30-31 1.2027517917156345 1102 32-33 1.1289481798781935 1102 34-35 0.9481141162529454 1102 36-37 0.9476600977060698 1102 38-39 0.8834585717581405 1102 40-41 0.8240459059057059 1102 42-43 0.6971962789153423 1102 44-45 0.6492233695633658 1102 46-47 0.6756758310392641 1102 48-49 0.81909231430717 1102 50-51 0.7489900325318786 1102 52-53 0.7867576338060296 1102 54-55 0.8259624570429445 1102 56-57 0.7215170439030416 1102 58-59 0.7179012270696852 1102 60-61 0.6321880354080989 1102 62-63 0.858338973424214 1102 64-65 0.8433839833397734 1102 66-67 0.7784007240469464 1102 68-69 0.5620203070845378 1102 70-71 0.43089472702278186 1102 72-73 0.5365995472290876 1102 74-75 0.6623936167689415 1102 76-77 0.6989809630827466 1102 78-79 0.6313306489331509 1102 80-81 0.6334427060633558 1102 82-83 0.6169508360645786 1102 84-85 0.6374961213539585 1102 86-87 0.5961485863298321 1102 88-89 0.7223489178123543 1102 90-91 0.6829241406663478 1102 92-93 0.7959000609086466 1102 94-95 0.5530913465254841 1102 96-97 0.5712868533374511 1102 98-99 0.6321988842487976 1102 100-101 0.48499591886039894 1102 102-103 0.4630306485837927 1102 104-105 0.486123584934532 1102 106-107 0.7515009160686432 1102 108-109 0.8034582859878903 1102 110-111 0.7296398823886641 1102 112-113 0.6896167652244181 1102 114-115 0.7194189414060332 1102 116-117 0.7001800608239748 1102 118-119 0.7563854520223643 1102 120-121 0.7803155603842988 1102 122-123 0.8398305366955121 1102 124-125 0.7190885344037845 1103 1 0.3997212680780713 1103 2 -0.8313030118906433 1103 3 0.19408442026957573 1103 4 0.34291088821623106 1103 5 0.5882608029421448 1103 6 0.7261822657420041 1103 7 0.6266608290924225 1103 8 0.7515919602416972 1103 9 0.9404201565395596 1103 10-11 1.05337142297644 1103 12-13 1.0419167452913882 1103 14-15 1.1795147343088175 1103 16-17 1.139326521791162 1103 18-19 1.2323745961647035 1103 20-21 1.2063732174717643 1103 22-23 1.0664017611190957 1103 24-25 1.03044615958909 1103 26-27 1.0561597474654718 1103 28-29 1.0841618383864002 1103 30-31 1.158239351296956 1103 32-33 1.0718945159610058 1103 34-35 1.053685288654222 1103 36-37 1.2442114069718428 1103 38-39 1.1703182396322873 1103 40-41 1.2100738912517812 1103 42-43 1.2160141375815385 1103 44-45 1.3007640278709331 1103 46-47 1.2248428583338224 1103 48-49 1.2117298161258532 1103 50-51 1.352289404666294 1103 52-53 1.200170955027236 1103 54-55 1.2913672058904915 1103 56-57 1.3762093101757529 1103 58-59 1.3365188906237613 1103 60-61 1.2672767560868792 1103 62-63 1.297312729040371 1103 64-65 1.3154457365136096 1103 66-67 1.2137308167034426 1103 68-69 1.139830826351396 1103 70-71 1.213007863453285 1103 72-73 1.2275611086483735 1103 74-75 1.1989554557473454 1103 76-77 1.0713536645462725 1103 78-79 1.1367326060234078 1103 80-81 1.1744904762016226 1103 82-83 1.2086621615213033 1103 84-85 1.1490560328384696 1103 86-87 1.078801063532552 1103 88-89 1.0449393483647498 1103 90-91 1.109329159363039 1103 92-93 1.1709131958469001 1103 94-95 1.082806540648992 1103 96-97 1.0482089222720958 1103 98-99 1.177066745215555 1103 100-101 1.1540490250479003 1103 102-103 1.1769438435852386 1103 104-105 1.1276477258629392 1103 106-107 1.0653142544507688 1103 108-109 1.0901408264994608 1103 110-111 1.1590224128275217 1103 112-113 1.085075169906112 1103 114-115 1.007910139598419 1103 116-117 1.0568114325902975 1103 118-119 1.0396187111160913 1103 120-121 1.076228309575061 1103 122-123 1.1106410976211087 1103 124-125 1.0151611567778858 1104 1 0.22544040324562786 1104 2 -0.7647539156182717 1104 3 0.09403963252046665 1104 4 0.12276952803189545 1104 5 0.48523955985541534 1104 6 0.5051205663287632 1104 7 0.5204722006774105 1104 8 0.6099397518181391 1104 9 0.6105663493281455 1104 10-11 0.7155057935760709 1104 12-13 0.6359912372437648 1104 14-15 0.7116176248188566 1104 16-17 0.8387782088448859 1104 18-19 0.778622733773993 1104 20-21 0.8043657184340702 1104 22-23 0.777559991610886 1104 24-25 0.7981529824144928 1104 26-27 0.6524453664776573 1104 28-29 0.8491022058879523 1104 30-31 0.7031619308323585 1104 32-33 0.7304500788858732 1104 34-35 0.7854007360316047 1104 36-37 0.7885260433601076 1104 38-39 0.8556700187582251 1104 40-41 0.9327938785200445 1104 42-43 0.9423546338402176 1104 44-45 0.9355080246812619 1104 46-47 0.8729673100706563 1104 48-49 0.9476227856593056 1104 50-51 0.9524341377173613 1104 52-53 0.8348480152905005 1104 54-55 0.960464738872826 1104 56-57 1.0110206264653385 1104 58-59 0.9043246937619962 1104 60-61 0.8310358084124161 1104 62-63 0.8748807374146388 1104 64-65 0.8370755081742054 1104 66-67 0.8096181479433113 1104 68-69 0.9176654780692068 1104 70-71 0.9116342550480141 1104 72-73 0.8500746341963037 1104 74-75 0.8231707319540362 1104 76-77 0.8837456106551542 1104 78-79 0.8090983886732417 1104 80-81 0.7134054240516186 1104 82-83 0.7001230034115125 1104 84-85 0.7336145832471246 1104 86-87 0.7717157534394232 1104 88-89 0.8110981295130273 1104 90-91 0.876909193339845 1104 92-93 0.8347819812212158 1104 94-95 0.7708511755315719 1104 96-97 0.7214619205162762 1104 98-99 0.7036564843789925 1104 100-101 0.7475014053620619 1104 102-103 0.7846188998877572 1104 104-105 0.8167281157369253 1104 106-107 0.8129856820878416 1104 108-109 0.7644497985547538 1104 110-111 0.6954728473866325 1104 112-113 0.7006897507998495 1104 114-115 0.7812571706715268 1104 116-117 0.7762461297085466 1104 118-119 0.7386793861091334 1104 120-121 0.7686204502134935 1104 122-123 0.8625696973100752 1104 124-125 0.8777945722680442 1105 1 0.4383133632473495 1105 2 0.14429508402560742 1105 3 0.3137902786346132 1105 4 0.3045249474014895 1105 5 0.4838373023087854 1105 6 0.31167959280784885 1105 7 0.3909567307137536 1105 8 0.42052883276878106 1105 9 0.47175441460809253 1105 10-11 0.6357501763776128 1105 12-13 0.6414639430518037 1105 14-15 0.5637221972168405 1105 16-17 0.7953797252643042 1105 18-19 0.6923236956012317 1105 20-21 0.700788917344731 1105 22-23 0.5792224552844445 1105 24-25 0.664886260914713 1105 26-27 0.7046688301448043 1105 28-29 0.8059730142396688 1105 30-31 0.7275785408184205 1105 32-33 0.6144414642295075 1105 34-35 0.69584654770658 1105 36-37 0.6524148605538613 1105 38-39 0.6305335339981326 1105 40-41 0.613121379283541 1105 42-43 0.6200119670921893 1105 44-45 0.7880813216932481 1105 46-47 0.871577659265462 1105 48-49 0.832735425029071 1105 50-51 0.6889934118555061 1105 52-53 0.6425608196966799 1105 54-55 0.6070829692951172 1105 56-57 0.6290177494428306 1105 58-59 0.5643203552526366 1105 60-61 0.6161522969411024 1105 62-63 0.6863826508792812 1105 64-65 0.646780476110564 1105 66-67 0.5942136572877423 1105 68-69 0.4959370430939565 1105 70-71 0.4656080416789905 1105 72-73 0.4404412113824563 1105 74-75 0.5035960655067804 1105 76-77 0.5304403766792589 1105 78-79 0.5933141665546913 1105 80-81 0.33366180190654404 1105 82-83 0.35079844128690496 1105 84-85 0.31971941046539243 1105 86-87 0.3429599552708176 1105 88-89 0.43117998978467753 1105 90-91 0.4424508960675091 1105 92-93 0.26166858753565947 1105 94-95 0.3101913327174941 1105 96-97 0.4685642721882637 1105 98-99 0.47674136743393447 1105 100-101 0.4456798654985761 1105 102-103 0.13390776777701419 1105 104-105 0.01725583094929206 1105 106-107 0.32323697696429576 1105 108-109 0.354623395622113 1105 110-111 0.2673344811636298 1105 112-113 0.07420112961482417 1105 114-115 0.21853270167269656 1105 116-117 0.008171419324188633 1105 118-119 -0.12365779484147055 1105 120-121 -0.08631213505022117 1105 122-123 -0.04537132641402053 1105 124-125 -0.17242132377363362 1106 1 0.3897795967792028 1106 2 0.35941538721997546 1106 3 0.4693660952742107 1106 4 0.3444185406157061 1106 5 0.433548184820971 1106 6 0.45479816346296076 1106 7 0.44448021023008977 1106 8 0.49145870102361044 1106 9 0.535602961801743 1106 10-11 0.7105122048923178 1106 12-13 0.5777682924472174 1106 14-15 0.7977144007901984 1106 16-17 0.8906654137681826 1106 18-19 0.7073409848991901 1106 20-21 0.7678523355002937 1106 22-23 0.6619980553566336 1106 24-25 0.6972308918477523 1106 26-27 0.7211654913972794 1106 28-29 0.8263960425739043 1106 30-31 0.7173202480914753 1106 32-33 0.5481656295426234 1106 34-35 0.6176904026064136 1106 36-37 0.6618484645964458 1106 38-39 0.7919442540847612 1106 40-41 0.7042470244739434 1106 42-43 0.6655472856267508 1106 44-45 0.6436273964090091 1106 46-47 0.42599238584910637 1106 48-49 0.49784980871432083 1106 50-51 0.5476540146347872 1106 52-53 0.583705955953679 1106 54-55 0.6344300185641991 1106 56-57 0.42686737947351006 1106 58-59 0.44399608165581483 1106 60-61 0.5625585052067592 1106 62-63 0.5167074298000536 1106 64-65 0.4238470705880957 1106 66-67 0.2890101200201016 1106 68-69 0.3600012914245845 1106 70-71 0.5067188880966071 1106 72-73 0.4635433951038088 1106 74-75 0.4642555854490311 1106 76-77 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-2.5185329594019805 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 95546.0 3 30753.0 4 13934.0 5 10836.0 6 12167.0 7 14580.0 8 16099.0 9 16982.0 10 18638.0 11 20836.0 12 24230.0 13 28293.0 14 32353.0 15 26728.0 16 16394.0 17 13598.0 18 12353.0 19 11484.0 20 10980.0 21 11497.0 22 13382.0 23 17439.0 24 23783.0 25 31885.0 26 38160.0 27 41670.0 28 47451.0 29 55419.0 30 64693.0 31 75792.0 32 91343.0 33 114072.0 34 147122.0 35 200204.0 36 306906.0 37 497179.0 38 222778.0 39 936.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.30204592884675 20.022290756340606 13.818744424735735 23.856918890076905 2 15.133251136524722 21.109854716041895 39.806263330882814 23.950630816550568 3 18.94243142357715 26.343640814578578 29.47500406630444 25.238923695539828 4 12.255625257125491 17.543051918449574 37.400284727229746 32.80103809719518 5 12.412304838103774 39.60546651025156 33.91323080674606 14.068997844898604 6 30.45599997035191 38.28404269819028 17.323666958068955 13.936290373388863 7 27.12601837763718 34.191381905254076 20.888122067370944 17.7944776497378 8 25.887308806483027 36.46785354715575 19.104877712328005 18.539959934033217 9 26.490995594771704 16.479498681890465 20.136151120166623 36.893354603171204 10-11 24.187231128579096 28.09346599957232 26.97135979873451 20.747943073114072 12-13 25.49049929166804 25.396357032253814 28.128768161302016 20.984375514776136 14-15 22.484830502524286 26.275243631323097 26.929525692863415 24.310400173289207 16-17 20.97229692690953 29.615522241213405 27.541463762326963 21.870717069550103 18-19 20.843482813706704 27.47867450994764 30.993450458216742 20.684392218128917 20-21 21.86896359019839 26.522840575257696 31.11729164431674 20.490904190227173 22-23 21.349381901027563 26.56182369363607 30.907254884437712 21.181539520898653 24-25 20.978466087020976 26.712428891144512 31.239141937702158 21.069963084132354 26-27 21.077354493214933 26.82920903687263 31.1461007743479 20.947335695564536 28-29 20.75015861767784 26.67232471716614 31.373938085546428 21.203578579609594 30-31 20.99034587264952 26.65183580777395 31.389852563007132 20.9679657565694 32-33 20.84235762787697 26.65190659605497 31.23826258334454 21.267473192723518 34-35 21.133870977704298 26.661986127210476 31.189852151229463 21.014290743855764 36-37 21.277435509836256 26.450546131434322 31.061146831745894 21.21087152698353 38-39 21.18392255285681 26.708743481044845 31.017173187509133 21.09016077858921 40-41 21.30444987533431 26.62657324804045 30.952071138709364 21.11690573791587 42-43 21.173992322535852 26.65170074526253 30.76437794998036 21.40992898222126 44-45 21.31365310614187 26.909896870283863 30.351802247894273 21.42464777568 46-47 21.47142281241906 27.076462645764266 29.690552947856965 21.761561593959712 48-49 21.559611116372725 27.216274310373933 29.367981749976323 21.856132823277015 50-51 21.778407984250236 27.47575542077537 29.000206301799352 21.745630293175033 52-53 21.789267123232673 27.62677983431278 28.870556063301024 21.713396979153522 54-55 21.63411907374732 27.517989536729537 29.015666081256086 21.832225308267052 56-57 21.654104810497397 27.6501734954752 28.854686910310466 21.841034783716935 58-59 21.702499223284217 27.28309658514771 28.97855029113716 22.035853900430904 60-61 21.743796054758192 27.386529517252455 29.119022275112776 21.750652152876576 62-63 21.790038686511604 27.343807584532808 29.07996516360956 21.786188565346027 64-65 21.687684658067305 27.26801799483215 29.13095667033838 21.913340676762168 66-67 21.662984688047533 27.464376084776788 29.004012773343163 21.86862645383252 68-69 21.723153349546507 27.372329500205478 28.984635644218315 21.919881506029704 70-71 21.554954817695734 27.33462494260849 29.10104818004567 22.00937205965011 72-73 21.772965155662362 27.402407070579375 28.85785419297826 21.966773580780004 74-75 21.613537121500375 27.54632260529961 28.83478202144289 22.00535825175712 76-77 21.685663460271446 27.411302543154566 28.975779088153907 21.927254908420082 78-79 21.723514932771387 27.434091664805067 28.861555693045098 21.980837709378445 80-81 21.47258084607204 27.70616739226443 28.816769492568845 22.004482269094684 82-83 21.663747154608135 27.728549616057396 28.655813823251343 21.95188940608313 84-85 21.577823430270072 27.433393823571922 28.698685727062607 22.2900970190954 86-87 21.51015675217821 27.40717147289069 28.807943065691628 22.274728709239472 88-89 21.705480143051563 27.242922056664725 28.85735815803615 22.194239642247567 90-91 21.610483035789656 27.534151810071673 28.883444273099183 21.97192088103949 92-93 21.555400257216878 27.516337554528864 28.868744031320663 22.059518156933592 94-95 21.379147468829963 27.534274019610567 28.805022044953876 22.281556466605597 96-97 21.567579765953706 27.3864514728889 28.627040142518105 22.418928618639285 98-99 21.45192389850801 27.404155089578097 28.483313035896096 22.660607976017797 100-101 21.367355951710586 27.454922981738594 28.740355345364854 22.437365721185966 102-103 21.536705780764905 27.274707550174625 28.77203500853513 22.416551660525343 104-105 21.414251762986577 27.15257722067542 28.907770853231767 22.52540016310624 106-107 21.281523284934195 27.425816391850795 28.66587999794925 22.62678032526576 108-109 21.343828024011476 27.41073219183977 28.415122435541328 22.830317348607423 110-111 21.27509186831577 27.4462873011716 28.541439157726906 22.73718167278573 112-113 21.331252954615458 27.314544861563395 28.517021879390743 22.837180304430397 114-115 21.15071860719606 27.39793265959298 28.483260637043916 22.96808809616705 116-117 21.019605178633196 27.407039086821168 28.55162161716987 23.021734117375765 118-119 21.044853099382212 27.419369118493346 28.50494816089836 23.030829621226083 120-121 20.932330988084864 27.473074395059328 28.469770675223995 23.12482394163181 122-123 21.00781562053474 27.460952780969087 28.293205186804858 23.238026411691312 124-125 20.85937292497406 27.38751133189358 28.3906888947134 23.36242684841895 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 315.0 1 292.0 2 522.0 3 892.0 4 696.5 5 542.0 6 1427.5 7 3306.0 8 5071.5 9 5538.5 10 4344.5 11 2898.5 12 2553.0 13 2703.5 14 2991.0 15 3288.0 16 3577.0 17 3866.5 18 4225.0 19 4802.0 20 5375.5 21 6070.5 22 7196.5 23 8660.0 24 10170.5 25 12303.0 26 15850.5 27 19726.5 28 22822.5 29 26807.5 30 32411.0 31 37492.0 32 42884.0 33 49423.5 34 56941.5 35 63060.5 36 68825.5 37 75143.0 38 78049.5 39 79532.0 40 81165.5 41 82965.0 42 84894.5 43 87141.5 44 89750.0 45 91488.0 46 91534.5 47 90915.0 48 93564.0 49 92366.5 50 86880.5 51 80479.0 52 73764.5 53 70239.0 54 66334.5 55 60581.0 56 53737.5 57 47848.0 58 43412.0 59 38456.0 60 32499.0 61 26357.0 62 20879.5 63 16110.0 64 12238.5 65 9739.0 66 7297.5 67 5304.5 68 4111.5 69 3082.0 70 2283.0 71 1836.5 72 1552.0 73 1441.0 74 1175.5 75 842.5 76 599.0 77 371.0 78 251.5 79 167.0 80 116.5 81 93.0 82 59.5 83 43.5 84 33.0 85 24.0 86 17.5 87 6.5 88 1.0 89 1.0 90 1.0 91 1.0 92 0.5 93 0.0 94 1.0 95 1.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06094309438561743 2 0.07407880189170658 3 0.0 4 0.00848261989421432 5 0.03158334688768147 6 3.2942213181414825E-4 7 0.0 8 0.0 9 7.000220301050652E-4 10-11 0.05979011692426791 12-13 0.010500330451575977 14-15 0.007597297914963794 16-17 0.055404684794492065 18-19 1.852999491454584E-4 20-21 0.010767985933674971 22-23 0.013341596338473004 24-25 0.0 26-27 0.0 28-29 0.05250165225787988 30-31 0.0 32-33 0.003973654465008163 34-35 0.0 36-37 1.852999491454584E-4 38-39 0.0 40-41 0.0 42-43 0.0036648212164323997 44-45 0.0 46-47 8.235553295353706E-5 48-49 2.0588883238384267E-4 50-51 5.764887306747594E-4 52-53 1.4412218266868985E-4 54-55 0.0 56-57 0.0018735883746929683 58-59 2.0588883238384266E-5 60-61 0.0 62-63 0.0 64-65 8.235553295353707E-4 66-67 0.0 68-69 7.000220301050652E-4 70-71 0.0 72-73 0.0027177325874667233 74-75 0.0037471767493859366 76-77 6.176664971515281E-4 78-79 0.0011323885781111347 80-81 0.0015441662428788202 82-83 2.882443653373797E-4 84-85 1.6471106590707413E-4 86-87 4.941331977212224E-4 88-89 0.0 90-91 0.0 92-93 0.007988486696493095 94-95 0.006650209285998119 96-97 0.007082575834004188 98-99 0.0011323885781111347 100-101 5.147220809596066E-4 102-103 6.17666497151528E-5 104-105 6.794331468666808E-4 106-107 0.004591320962159692 108-109 0.006423731570375892 110-111 7.823775630586021E-4 112-113 0.0037471767493859366 114-115 5.147220809596066E-4 116-117 0.0022647771562222694 118-119 0.002779499237181876 120-121 0.013300418571996238 122-123 0.0010294441619192133 124-125 0.002532432638321265 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2428495.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.889189429584825 #Duplication Level Percentage of deduplicated Percentage of total 1 74.61789124330296 39.46479784803209 2 12.51817935421085 13.241527183567511 3 5.05301767679902 8.017500272977921 4 2.5198209559083895 5.330851514627052 5 1.478169835236612 3.908960221246368 6 0.9595044542835727 3.0448447704680572 7 0.612530259240207 2.267736023861561 8 0.42400048939396984 1.7940033761435474 9 0.30730963328128713 1.4628021667335216 >10 1.3882258394270832 12.65466279862338 >50 0.07995766344365117 2.890370267965828 >100 0.038961534813955534 3.5837532061807926 >500 0.0016468475428375446 0.5991680169926603 >1k 5.489491809451525E-4 0.49991500130178457 >5k 7.842131156359322E-5 0.2977590470708961 >10k+ 1.5684262312718643E-4 0.9413482842068616 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12605 0.5190457464396674 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10091 0.41552484151707125 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7179 0.2956151855367213 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2880 0.11859196745309339 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2851 0.11739781222526709 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.117776647676853E-5 0.0 5 0.0 0.0 0.0 4.117776647676853E-5 0.0 6 4.117776647676853E-5 0.0 0.0 4.117776647676853E-5 0.0 7 4.117776647676853E-5 0.0 0.0 8.235553295353706E-5 0.0 8 8.235553295353706E-5 0.0 0.0 8.235553295353706E-5 0.0 9 8.235553295353706E-5 0.0 0.0 8.235553295353706E-5 0.0 10-11 8.235553295353706E-5 0.0 0.0 1.0294441619192134E-4 0.0 12-13 1.0294441619192134E-4 0.0 0.0 1.4412218266868985E-4 0.0 14-15 2.470665988606112E-4 0.0 0.0 1.6471106590707413E-4 0.0 16-17 3.0883324857576403E-4 0.0 0.0 1.852999491454584E-4 0.0 18-19 4.529554312444539E-4 0.0 0.0 2.470665988606112E-4 0.0 20-21 5.35310964197991E-4 0.0 0.0 3.2942213181414825E-4 0.0 22-23 6.588442636282965E-4 0.0 0.0 5.147220809596066E-4 0.0 24-25 7.00022030105065E-4 0.0 0.0 7.823775630586021E-4 0.0 26-27 7.206109133434493E-4 0.0 0.0 0.0014823995931636672 0.0 28-29 0.0011117996948727504 0.0 0.0 0.0022235993897455008 0.0 30-31 0.001317688527256593 0.0 0.0 0.004076598881200085 0.0 32-33 0.001317688527256593 0.0 0.0 0.0076796534479173315 0.0 34-35 0.0015029884764020513 0.0 0.0 0.012579807658652787 0.0 36-37 0.0016471106590707414 0.0 0.0 0.021350671918204486 0.0 38-39 0.0018118217249778155 0.0 0.0 0.03662762328108561 0.0 40-41 0.0018118217249778155 0.0 0.0 0.056331184540219355 0.0 42-43 0.001935355024408121 0.0 0.0 0.07527295711953289 0.0 44-45 0.0023471326891758063 0.0 0.0 0.09674716233716768 0.0 46-47 0.0026559659377515706 0.0 0.0 0.1217008888220894 0.0 48-49 0.002841265886897029 0.0 0.0 0.15254303591318905 0.0 50-51 0.002841265886897029 0.0 0.0 0.18929419249370494 0.0 52-53 0.0028618547701354134 0.0 0.0 0.22425411623248143 0.0 54-55 0.0028824436533737975 0.0 0.0 0.2588228511897286 0.0 56-57 0.0029236214198505657 0.0 0.0 0.3005976952804103 0.0 58-59 0.003005976952804103 0.0 0.0 0.3496404151542416 0.0 60-61 0.003005976952804103 0.0 0.0 0.4088746322310731 0.0 62-63 0.003005976952804103 0.0 0.0 0.46689410519684005 2.0588883238384266E-5 64-65 0.003005976952804103 0.0 0.0 0.5259018445580493 4.117776647676853E-5 66-67 0.003253043551664714 0.0 0.0 0.5934333815799497 4.117776647676853E-5 68-69 0.0032942213181414827 0.0 0.0 0.67778603620761 4.117776647676853E-5 70-71 0.0032942213181414827 0.0 0.0 0.7719595881399797 4.117776647676853E-5 72-73 0.0033559879678566355 0.0 0.0 0.8730510048404465 4.117776647676853E-5 74-75 0.0034177546175717883 0.0 0.0 0.971712933318784 4.117776647676853E-5 76-77 0.0034177546175717883 0.0 0.0 1.0784868817930446 4.117776647676853E-5 78-79 0.003479521267286941 0.0 0.0 1.205808535739213 4.117776647676853E-5 80-81 0.004035421114723317 0.0 0.0 1.3546867504359696 4.117776647676853E-5 82-83 0.004158954414153622 0.0 0.0 1.5220126045143187 4.117776647676853E-5 84-85 0.004179543297392006 0.0 0.0 1.6843559488489785 4.117776647676853E-5 86-87 0.0042001321806303905 4.117776647676853E-5 0.0 1.8542554133321256 4.117776647676853E-5 88-89 0.0042001321806303905 4.117776647676853E-5 0.0 2.0509410149084104 4.117776647676853E-5 90-91 0.004611909845398076 4.117776647676853E-5 0.0 2.2738774426136352 4.117776647676853E-5 92-93 0.004756032028066766 4.117776647676853E-5 0.0 2.517670409039343 4.117776647676853E-5 94-95 0.00477662091130515 4.117776647676853E-5 0.0 2.765745863178635 4.117776647676853E-5 96-97 0.00477662091130515 4.117776647676853E-5 0.0 3.0169508275701618 4.117776647676853E-5 98-99 0.005106043043119297 4.117776647676853E-5 0.0 3.2926565630153655 4.117776647676853E-5 100-101 0.005579587357602136 4.117776647676853E-5 0.0 3.622943427925526 4.117776647676853E-5 102-103 0.0059913650223698215 4.117776647676853E-5 0.0 3.9785752081021375 4.117776647676853E-5 104-105 0.006959042534573882 4.117776647676853E-5 0.0 4.337233554114791 4.117776647676853E-5 106-107 0.007020809184289035 4.117776647676853E-5 0.0 4.686297480538357 4.117776647676853E-5 108-109 0.007103164717242572 6.176664971515279E-5 0.0 5.068015375778002 4.117776647676853E-5 110-111 0.007164931366957725 8.235553295353706E-5 0.0 5.502337867691718 4.117776647676853E-5 112-113 0.0074325868490567205 8.235553295353706E-5 0.0 5.980082314355187 4.117776647676853E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 15250 0.0 41.06297 1 GGTATCA 10305 0.0 38.218266 1 ATCAACG 19205 0.0 32.036266 3 TCAACGC 19265 0.0 31.998926 4 TATCAAC 19405 0.0 31.82298 2 CAACGCA 19490 0.0 30.955372 5 AACGCAG 19725 0.0 30.66704 6 GTACATG 13830 0.0 30.631262 1 TACATGG 14025 0.0 28.787407 2 ACATGGG 14240 0.0 26.826294 3 ACGCAGA 23025 0.0 26.065973 7 CGCAGAG 23015 0.0 25.922077 8 GAGTACT 12975 0.0 25.430197 12-13 AACGCCG 375 0.0 25.402935 6 AGTACTT 13570 0.0 24.24933 12-13 GTACTTT 13720 0.0 24.027626 14-15 GCAGAGT 24310 0.0 23.904896 9 CATGGGA 10655 0.0 23.008505 4 AGAGTAC 23065 0.0 21.051199 10-11 TACTTTT 16030 0.0 20.602285 14-15 >>END_MODULE