FastQCFastQC Report
Thu 26 May 2016
SRR1364591_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364591_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3108967
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31950.054.1908951
GTATCAA57250.052.2753141
ATCAACG65300.045.1005863
TCAACGC66850.044.1438754
CAACGCA68450.043.2858665
AACGCAG72100.040.5948686
TATCAAC73000.040.587912
GTACATG95650.033.901251
ACGCAGA84750.033.763447
CGCAGAG82800.033.2690858
TACATGG98050.032.220862
ACATGGG98100.030.6275733
GCAGAGT101250.025.913969
GTATAGG9800.023.6778741
CATGGGG61550.023.0058944
AGAGTAC88350.022.86616310-11
GAGTACA69950.022.5404361
CAGAGTA94850.022.24021310-11
GAGTACT54750.020.70712512-13
AGTACAT71100.020.417872
Produced by FastQC (version 0.11.5)