##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364591_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3108967 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.114001210048226 31.0 27.0 31.0 10.0 34.0 2 27.18125731151215 31.0 27.0 33.0 10.0 34.0 3 27.29568374318544 31.0 27.0 34.0 10.0 34.0 4 30.587214016745754 35.0 31.0 37.0 10.0 37.0 5 30.61603162722538 35.0 31.0 37.0 10.0 37.0 6 30.622262314138425 35.0 31.0 37.0 8.0 37.0 7 30.37890334635266 35.0 30.0 37.0 8.0 37.0 8 30.2589834501299 35.0 30.0 37.0 7.0 37.0 9 31.301993234408727 37.0 30.0 39.0 2.0 39.0 10-11 31.200663435797164 37.0 30.0 39.0 2.0 39.0 12-13 31.090761336482505 37.0 30.0 39.0 2.0 39.0 14-15 31.709175909554524 38.0 30.0 40.0 2.0 41.0 16-17 31.787569633257604 38.0 30.0 40.0 2.0 41.0 18-19 31.574873100936742 38.0 30.0 40.0 2.0 41.0 20-21 31.27069570053333 37.5 28.5 40.0 2.0 41.0 22-23 31.15144403269639 37.0 28.5 40.0 2.0 41.0 24-25 30.962611053768022 37.0 27.0 40.0 2.0 41.0 26-27 30.82201692716584 37.0 27.5 40.0 2.0 41.0 28-29 30.856969694435485 37.0 28.0 40.0 2.0 41.0 30-31 30.781475486873937 37.0 28.0 40.0 2.0 41.0 32-33 30.747278276031878 37.0 28.5 40.0 2.0 41.0 34-35 30.29419402007162 36.5 27.0 40.0 2.0 41.0 36-37 30.15307351284205 36.0 26.5 40.0 2.0 41.0 38-39 29.949761287270015 36.0 26.0 40.0 2.0 41.0 40-41 30.13465758883899 36.0 27.0 40.0 2.0 41.0 42-43 30.014570595313494 36.0 27.0 40.0 2.0 41.0 44-45 29.8687905017969 36.0 27.0 40.0 2.0 41.0 46-47 29.841647724147602 36.0 27.0 40.0 2.0 41.0 48-49 29.700470928125 36.0 27.0 40.0 2.0 41.0 50-51 29.54088303285303 36.0 26.5 40.0 2.0 41.0 52-53 29.46877998383386 35.5 26.5 40.0 2.0 41.0 54-55 29.489443278104915 36.0 26.5 40.0 2.0 41.0 56-57 29.355308853390852 35.5 26.0 40.0 2.0 41.0 58-59 29.15420604335781 35.0 26.0 39.5 2.0 41.0 60-61 28.95176018272307 35.0 26.0 39.0 2.0 41.0 62-63 28.662413271031824 35.0 26.0 39.0 2.0 41.0 64-65 28.392622533465296 34.5 25.0 39.0 2.0 40.5 66-67 28.152683672744033 34.0 25.0 38.5 2.0 40.0 68-69 28.110077237873544 34.0 25.5 38.0 2.0 40.0 70-71 27.88662198730318 34.0 25.0 37.5 2.0 40.0 72-73 27.61776194472312 34.0 25.0 37.0 2.0 39.5 74-75 27.333744616781072 34.0 24.0 36.5 2.0 39.0 76-77 27.0399595749971 34.0 24.0 36.0 2.0 39.0 78-79 26.742151814412953 34.0 23.0 35.5 2.0 38.5 80-81 26.45624543457682 33.0 22.0 35.0 2.0 37.0 82-83 26.212826157369957 33.0 22.0 35.0 2.0 37.0 84-85 25.97989010497699 33.0 20.5 35.0 2.0 36.0 86-87 25.79195710343661 33.0 20.0 35.0 2.0 36.0 88-89 25.59900571475992 33.0 20.0 35.0 2.0 36.0 90-91 25.461990429618584 33.0 19.5 35.0 2.0 35.5 92-93 25.340442339851148 33.0 18.5 35.0 2.0 35.0 94-95 25.214715209264043 33.0 18.0 35.0 2.0 35.0 96-97 25.101150317774362 33.0 17.5 35.0 2.0 35.0 98-99 25.005998294610396 33.0 17.0 35.0 2.0 35.0 100-101 24.910170645104948 33.0 14.0 35.0 2.0 35.0 102-103 23.826931421272725 30.5 10.0 34.0 2.0 35.0 104-105 24.648520553611537 32.0 10.0 35.0 2.0 35.0 106-107 24.7945709941598 33.0 9.0 35.0 2.0 35.0 108-109 24.75153258300908 33.0 7.0 35.0 2.0 35.0 110-111 24.662123142510037 33.0 4.5 35.0 2.0 35.0 112-113 24.565098793264774 33.0 2.0 35.0 2.0 35.0 114-115 24.43841041091784 33.0 2.0 35.0 2.0 35.0 116-117 24.329958471736752 33.0 2.0 35.0 2.0 35.0 118-119 24.175244060165323 33.0 2.0 35.0 2.0 35.0 120-121 23.995490946028056 33.0 2.0 35.0 2.0 35.0 122-123 23.752022295508443 32.0 2.0 35.0 2.0 35.0 124-125 22.395074151639434 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5543125457832616 1101 2 0.4980247286212496 1101 3 0.5746171816013472 1101 4 0.5304832308582057 1101 5 0.5605995928396403 1101 6 0.5987321988218177 1101 7 0.7385289399704718 1101 8 0.6971891120949465 1101 9 0.8336482559941665 1101 10-11 0.9071259413845638 1101 12-13 0.7802133566929257 1101 14-15 0.9868043241984275 1101 16-17 0.8507241293675278 1101 18-19 1.2194379863382743 1101 20-21 1.620914473338555 1101 22-23 1.4174743474932612 1101 24-25 1.4944873280429434 1101 26-27 1.647101378493808 1101 28-29 1.4544533008228449 1101 30-31 1.524275140786738 1101 32-33 1.53014957914624 1101 34-35 1.8640929289815915 1101 36-37 1.78501274318306 1101 38-39 1.598976283271508 1101 40-41 1.3116309236689787 1101 42-43 1.3037766438324354 1101 44-45 1.31149238645801 1101 46-47 1.2549836843506128 1101 48-49 1.200821739363498 1101 50-51 1.1245964645900095 1101 52-53 0.9835079008217704 1101 54-55 0.9341429678276398 1101 56-57 0.983596897603455 1101 58-59 1.018997417523586 1101 60-61 1.1276986037967909 1101 62-63 1.0545614853158014 1101 64-65 1.1955360780803126 1101 66-67 1.078610250485891 1101 68-69 0.9376770526311766 1101 70-71 0.8526444750724025 1101 72-73 0.7899048107939635 1101 74-75 0.7846281915256164 1101 76-77 0.9934867633820552 1101 78-79 1.0190059554001607 1101 80-81 1.0940122659127063 1101 82-83 0.9381841630568424 1101 84-85 1.0336776081197208 1101 86-87 1.0105591712706037 1101 88-89 0.9173568550009286 1101 90-91 0.9187971268615378 1101 92-93 0.8653840533241066 1101 94-95 0.9274249738819584 1101 96-97 0.8295874280312461 1101 98-99 0.8784883088709883 1101 100-101 0.9201366937663522 1101 102-103 0.8854787613879935 1101 104-105 0.9317935247908373 1101 106-107 0.9138542418445219 1101 108-109 1.0160974073221034 1101 110-111 0.845602809342445 1101 112-113 0.8788495277764028 1101 114-115 1.0579678118631328 1101 116-117 1.1454426013995302 1101 118-119 1.083357822630127 1101 120-121 1.1698854045684435 1101 122-123 1.206228592937837 1101 124-125 1.4609220727686711 1102 1 -0.1504486948794579 1102 2 0.11355299093009918 1102 3 0.20852521899086796 1102 4 0.25893233604911714 1102 5 0.30036974442212383 1102 6 -0.4620281726088571 1102 7 -1.1648774645651798 1102 8 -0.21159981633859104 1102 9 0.11941862119522284 1102 10-11 0.06478530166888063 1102 12-13 0.27749746773252326 1102 14-15 0.4579583459554222 1102 16-17 0.36064600178779926 1102 18-19 0.5328497737667668 1102 20-21 0.9226053713242486 1102 22-23 0.7138877322647659 1102 24-25 0.8151520009362798 1102 26-27 0.29274289553984545 1102 28-29 0.87926763437072 1102 30-31 0.7851796494114538 1102 32-33 0.6681501738839515 1102 34-35 1.0315187440295972 1102 36-37 1.0510998080620482 1102 38-39 1.1275223561670913 1102 40-41 0.9224037285121796 1102 42-43 0.7877548820400158 1102 44-45 0.8534977206502461 1102 46-47 0.5067944153448813 1102 48-49 0.2354710682451504 1102 50-51 0.6531481638814718 1102 52-53 0.3825050557101761 1102 54-55 0.24117008724023492 1102 56-57 0.3061679963389565 1102 58-59 0.45846528406990217 1102 60-61 0.24737647141857977 1102 62-63 0.1427862780937943 1102 64-65 0.24019942314421527 1102 66-67 0.23950298979725915 1102 68-69 0.1397705976424355 1102 70-71 0.16461705810647587 1102 72-73 0.24027428053105737 1102 74-75 -0.18065247247026406 1102 76-77 0.03664421742501034 1102 78-79 -0.019462681685684657 1102 80-81 -0.2886197325628146 1102 82-83 -0.12563337052674584 1102 84-85 -0.46477716220618603 1102 86-87 0.058007997355385754 1102 88-89 0.29489641179219817 1102 90-91 -0.29460699575552596 1102 92-93 0.09583693945304717 1102 94-95 -0.023071181998354007 1102 96-97 0.12533334700339793 1102 98-99 0.2245622928407407 1102 100-101 0.25532175699504833 1102 102-103 0.04183773245485156 1102 104-105 0.314136132822707 1102 106-107 0.23771383135374435 1102 108-109 -0.23377692113065862 1102 110-111 -0.028094841425136963 1102 112-113 -0.36788697814321125 1102 114-115 -0.23830643490542514 1102 116-117 -0.32232345379780725 1102 118-119 -0.008186589833023561 1102 120-121 0.32704812012166684 1102 122-123 0.14071608453744489 1102 124-125 0.45035279587860444 1103 1 0.45663174875770807 1103 2 0.5093191025668347 1103 3 0.374896035811652 1103 4 0.47326504309566175 1103 5 0.47484900406485764 1103 6 0.6178111201184819 1103 7 0.7326965666985821 1103 8 0.6831283104837418 1103 9 0.8629058336388447 1103 10-11 0.9513828573652603 1103 12-13 0.905006984762764 1103 14-15 1.121450995639453 1103 16-17 0.9832521934732128 1103 18-19 1.0570561393546107 1103 20-21 1.3921036427132059 1103 22-23 1.4151496778298878 1103 24-25 1.4508145428126937 1103 26-27 1.3694064512272988 1103 28-29 1.3950006260727896 1103 30-31 1.3089090477744811 1103 32-33 1.3127405101746632 1103 34-35 1.5921389539026194 1103 36-37 1.5239679970072686 1103 38-39 1.6826529801034482 1103 40-41 1.411529666082842 1103 42-43 1.3119702198183525 1103 44-45 0.9508677438273203 1103 46-47 1.0670700559127937 1103 48-49 1.0583740674299378 1103 50-51 1.1075798030420998 1103 52-53 1.126855364985765 1103 54-55 1.0346921843042765 1103 56-57 1.0172473313294965 1103 58-59 0.8561593939244716 1103 60-61 0.8505732117520459 1103 62-63 0.7294037120733492 1103 64-65 0.9311585977250054 1103 66-67 0.9555564978958486 1103 68-69 1.067081629364381 1103 70-71 1.0163549574572777 1103 72-73 0.997705997047774 1103 74-75 1.0774435115586805 1103 76-77 1.1011141478491595 1103 78-79 1.0548534142566908 1103 80-81 0.9275499956197919 1103 82-83 1.0517979257381462 1103 84-85 1.054858639381397 1103 86-87 0.9184187042561405 1103 88-89 0.9031951673261354 1103 90-91 0.9677520339313617 1103 92-93 1.063347057108679 1103 94-95 1.000084832958148 1103 96-97 1.06269513807948 1103 98-99 0.9658428577930813 1103 100-101 0.9716238366093393 1103 102-103 0.8629840883136808 1103 104-105 0.8958161657287995 1103 106-107 0.9987731950850467 1103 108-109 1.1107253947172246 1103 110-111 1.0473055414131167 1103 112-113 1.0542442174100053 1103 114-115 1.1789134393611818 1103 116-117 1.1545897519277304 1103 118-119 1.2298256339164837 1103 120-121 1.2294827077393364 1103 122-123 1.1739936601232657 1103 124-125 1.2457604940738278 1104 1 0.5635646335326499 1104 2 0.5623756643012499 1104 3 0.4603327968570774 1104 4 0.4640649885763004 1104 5 0.4468232145852724 1104 6 0.4248393874960037 1104 7 0.5503076691669513 1104 8 0.6928267388825802 1104 9 0.8286945844307034 1104 10-11 0.8192416007150243 1104 12-13 0.6849090233523825 1104 14-15 0.7391118172420157 1104 16-17 0.908573085985978 1104 18-19 1.2056872908273384 1104 20-21 1.4062066730926794 1104 22-23 1.209256751611818 1104 24-25 1.522679298533724 1104 26-27 1.584708062246385 1104 28-29 1.4067218287432617 1104 30-31 1.2606137982818346 1104 32-33 1.363163692185239 1104 34-35 1.596968174457956 1104 36-37 1.669061543610809 1104 38-39 1.6765139021253432 1104 40-41 1.3678615546180808 1104 42-43 1.4234705025762189 1104 44-45 1.3429428414436053 1104 46-47 1.3210246366603116 1104 48-49 1.286566191764912 1104 50-51 1.1144231697247413 1104 52-53 1.2644389222128751 1104 54-55 1.0584377134363976 1104 56-57 1.0114884829704032 1104 58-59 0.9439034828134538 1104 60-61 0.942805183923344 1104 62-63 0.9951177089731758 1104 64-65 0.9732100270712785 1104 66-67 0.9556273625532867 1104 68-69 0.988720603629563 1104 70-71 0.9600820906849385 1104 72-73 0.896942651542183 1104 74-75 0.9259842777449059 1104 76-77 0.9201344627930688 1104 78-79 1.051193219661819 1104 80-81 1.0210484217061158 1104 82-83 0.8571569975705593 1104 84-85 0.8665702810168092 1104 86-87 0.891497996114115 1104 88-89 0.9146836728859888 1104 90-91 1.0217869483962332 1104 92-93 1.0694748133847156 1104 94-95 0.9974175319257839 1104 96-97 0.7687029675291868 1104 98-99 0.8564360825712178 1104 100-101 0.8832387685722622 1104 102-103 0.9093389250849597 1104 104-105 0.8899795983742642 1104 106-107 0.8679652057212266 1104 108-109 1.0043400029276448 1104 110-111 1.0308454728154288 1104 112-113 0.8779709441951304 1104 114-115 0.8286856738701118 1104 116-117 0.8763535145624886 1104 118-119 0.9420349749469992 1104 120-121 0.9283195419093815 1104 122-123 0.9429682853201804 1104 124-125 1.1100762454389148 1105 1 -0.018452578706348532 1105 2 0.29080911421058886 1105 3 0.21041841026466201 1105 4 0.3501750165980262 1105 5 0.3625312985180926 1105 6 0.3425332961911991 1105 7 0.37222758878651874 1105 8 0.16187911829561585 1105 9 0.08272922026781515 1105 10-11 0.3409607378217778 1105 12-13 0.321252248767653 1105 14-15 0.5286076280741376 1105 16-17 0.44542136459135406 1105 18-19 0.6493466881668617 1105 20-21 0.868689241908978 1105 22-23 1.0434805378626635 1105 24-25 1.1458146756880723 1105 26-27 0.9024894494096358 1105 28-29 0.5243976789849967 1105 30-31 0.4653941535890347 1105 32-33 0.5869390076797778 1105 34-35 1.2477246650304963 1105 36-37 1.2081282932823925 1105 38-39 1.3171477175346666 1105 40-41 0.9382361862686359 1105 42-43 0.6182685917342354 1105 44-45 0.6744871862928115 1105 46-47 0.7083098905067118 1105 48-49 0.5141288118727552 1105 50-51 0.6329220627259851 1105 52-53 0.605143220915771 1105 54-55 0.41303096406132056 1105 56-57 0.5161463796602277 1105 58-59 0.49736748362361993 1105 60-61 0.4782247354365943 1105 62-63 0.44409538136819293 1105 64-65 0.48985339020711294 1105 66-67 0.28947416021851424 1105 68-69 0.14072921325010412 1105 70-71 0.39272781965970793 1105 72-73 0.3810440807490494 1105 74-75 0.3371455257733551 1105 76-77 0.46491127988820935 1105 78-79 0.5110693286434156 1105 80-81 0.3843366361361156 1105 82-83 0.3327818890004828 1105 84-85 0.37940295604646934 1105 86-87 0.3779324798245334 1105 88-89 0.5550376427785118 1105 90-91 0.4150257216506503 1105 92-93 0.38348640113728294 1105 94-95 0.4110623143661556 1105 96-97 0.5444899139125638 1105 98-99 0.4390202256498412 1105 100-101 0.4981843258793468 1105 102-103 0.3622303285565813 1105 104-105 0.44508697031646705 1105 106-107 0.5068511158441567 1105 108-109 0.4495185492947549 1105 110-111 0.5222015334342593 1105 112-113 0.6614509948988427 1105 114-115 0.6065828616225737 1105 116-117 0.6027499207832925 1105 118-119 0.6644476941148341 1105 120-121 0.5006625363424249 1105 122-123 0.6408894106808134 1105 124-125 0.8082785994434722 1106 1 0.1579294182492319 1106 2 0.48566114906248004 1106 3 0.21530917140487205 1106 4 0.3866540146943791 1106 5 0.2633081199604135 1106 6 0.2531552566278741 1106 7 0.04471987178332881 1106 8 0.30581999051122466 1106 9 0.4493064291496083 1106 10-11 0.5814983807276093 1106 12-13 0.49835687596753075 1106 14-15 0.7798411124511766 1106 16-17 0.6746104876784358 1106 18-19 0.6104327283376669 1106 20-21 0.9965556582880986 1106 22-23 0.9275294304638777 1106 24-25 1.1811451514934106 1106 26-27 1.3960988613280776 1106 28-29 1.242713420965135 1106 30-31 0.9625189739082423 1106 32-33 0.8607915007839182 1106 34-35 1.1780113199126916 1106 36-37 1.0614644182166124 1106 38-39 1.2561563298514997 1106 40-41 0.9306457763668554 1106 42-43 0.9110662172988846 1106 44-45 0.7315716203733587 1106 46-47 0.5918381502783454 1106 48-49 0.7632261918000687 1106 50-51 0.7666130285345396 1106 52-53 0.6911197087787109 1106 54-55 0.520147979082104 1106 56-57 0.5828547952552867 1106 58-59 0.5463008993069707 1106 60-61 0.5635083926956987 1106 62-63 0.4466491884819632 1106 64-65 0.5068822291954582 1106 66-67 0.38286839665901695 1106 68-69 0.48563336436209426 1106 70-71 0.6233568613739102 1106 72-73 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-2.475350033124311 2216 118-119 -2.486217016743886 2216 120-121 -2.626167124435071 2216 122-123 -2.689205590401528 2216 124-125 -2.5938604906852056 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 293814.0 3 46120.0 4 27574.0 5 21239.0 6 19372.0 7 20446.0 8 21644.0 9 21877.0 10 22681.0 11 23644.0 12 24976.0 13 26674.0 14 27685.0 15 25071.0 16 20394.0 17 18525.0 18 17175.0 19 16316.0 20 16425.0 21 17449.0 22 20915.0 23 27103.0 24 35993.0 25 45835.0 26 54624.0 27 62459.0 28 71076.0 29 81691.0 30 92622.0 31 106015.0 32 127064.0 33 156413.0 34 198555.0 35 267374.0 36 394027.0 37 482410.0 38 155182.0 39 508.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.57173536741462 18.173392948076433 12.929244968920361 24.325626715588587 2 15.26691357595806 19.242133867552916 40.82800818570564 24.66294437078339 3 19.559720286378678 24.038882496539607 28.280039153988206 28.121358063093503 4 12.114883917525376 15.882747275582568 37.612754757718406 34.38961404917365 5 13.483743909555493 37.90213983461142 34.47524543534333 14.138870820489757 6 32.73119865008609 36.43365148045684 17.317837371527713 13.517312497929357 7 28.8502991430859 32.01849125345441 20.591415161193716 18.53979444226598 8 25.744145885112324 36.22399337143173 19.23848017685617 18.793380566599772 9 25.900978417432803 16.45123127230292 19.388735172398707 38.25905513786557 10-11 24.90999421994508 26.593013049028823 27.514058528122042 20.98293420290405 12-13 25.666218377002707 23.736985955913646 27.606660720207742 22.990134946875905 14-15 22.33230738742182 25.457083192985607 25.231577772265744 26.979031647326828 16-17 22.485242131362167 28.634753570057686 25.96102706464569 22.91897723393446 18-19 22.432086377195454 27.48678060083631 27.754650653543422 22.32648236842482 20-21 22.960143287684186 26.698831123253335 28.123794717489016 22.21723087157346 22-23 22.536979463778678 26.67174130964709 27.840056431145012 22.951222795429217 24-25 22.41628286494887 26.645205724905125 28.149777085646143 22.788734324499863 26-27 22.265737473864846 27.060820799128543 27.74187103750176 22.931570689504856 28-29 22.257016084714255 27.311173515264258 27.739775431894838 22.692034968126652 30-31 22.47400177779093 26.989758000742704 27.599279426951114 22.936960794515258 32-33 22.27989041666982 26.925199389002003 27.595708088099645 23.199202106228533 34-35 22.659382110138814 26.849396061411333 27.965899963231948 22.525321865217904 36-37 22.758406157856843 26.58331011827136 27.723770143221383 22.93451358065041 38-39 22.376290903055583 26.90866451782859 27.815380478467606 22.89966410064822 40-41 22.84430255371146 26.838791936894303 27.546472503862386 22.77043300553185 42-43 22.538449834189063 26.8073456706923 27.465201255460148 23.18900323965849 44-45 22.640668150895966 27.078139509297127 27.505409029778257 22.77578331002865 46-47 22.970186230713015 27.216829342981548 26.80173775874669 23.011246667558748 48-49 22.791782363150517 27.26642715798709 26.871467875695927 23.070322603166463 50-51 22.866250981273893 27.401992028220157 26.84147776591377 22.890279224592184 52-53 23.00186846627835 27.193099830265165 26.84972211027007 22.955309593186417 54-55 22.775317691117742 27.240212784404346 26.995859129659607 22.988610394818306 56-57 22.74370030977957 27.4854941105534 26.81610905218646 22.95469652748057 58-59 22.768223308259554 27.16193013865096 26.893238827928844 23.17660772516065 60-61 22.70227132400966 27.306325170068245 27.045229782115467 22.946173723806627 62-63 22.715135884524294 27.253623335286285 27.206617382807664 22.824623397381757 64-65 22.524248575647512 27.29204131989461 27.023883439549866 23.15982666490801 66-67 22.551458993231545 27.40454645241134 26.92733701469085 23.116657539666267 68-69 22.861619811993634 27.106159992973648 27.142739485487077 22.88948070954564 70-71 22.729959321239015 27.052139997468533 27.04234544346572 23.175555237826728 72-73 22.686402766275133 27.229644231964738 26.93066570974667 23.153287292013456 74-75 22.631234527615526 27.466297883878777 26.871051843112493 23.031415745393204 76-77 22.842042706635347 27.202951008719694 26.945694716636094 23.009311568008865 78-79 22.839545188230886 27.217861939516574 26.955746414084953 22.98684645816758 80-81 22.865639773305897 27.299388618139325 26.816703491538213 23.018268117016564 82-83 22.86794726266414 27.431819504991612 26.891920884346543 22.80831234799771 84-85 22.832101466499964 27.4810089653573 26.817508838144633 22.8693807299981 86-87 22.879715823802652 27.348726210689705 26.85132779771328 22.92023016779436 88-89 22.833224972196604 27.44776869889105 26.688157666579915 23.030848662332435 90-91 22.793579708326497 27.55486170763801 26.75406254926374 22.897496034771756 92-93 22.810296283691738 27.36708664502281 26.912169958225185 22.91044711306027 94-95 22.706646714928766 27.24253555230462 27.03952200766561 23.011295725101 96-97 22.932948182735494 27.080442974661032 26.992712797555136 22.993896045048338 98-99 22.64168088104599 27.240240326207545 26.78256036380234 23.335518428944134 100-101 22.67226559886357 27.165559876063472 27.0441099267601 23.118064598312856 102-103 22.516691235384613 26.826466797492543 27.29255408629297 23.36428788082987 104-105 22.43518783734514 27.03251427783835 27.14118522904932 23.39111265576719 106-107 22.51539057457877 27.108286376380303 26.92584621020922 23.450476838831708 108-109 22.54469088928895 27.02407262605232 26.851603764208498 23.579632720450235 110-111 22.55749656676305 27.043228309010786 27.04253675642215 23.356738367804013 112-113 22.56725465403782 27.102056728167266 27.13677887221061 23.193909745584303 114-115 22.486970752664792 27.255323070331723 26.96402052514549 23.293685651857995 116-117 22.40310045105014 27.208169144284906 27.07368717648016 23.315043228184795 118-119 22.331581518877492 27.26476350504846 26.906493378668863 23.497161597405185 120-121 22.2857928603249 27.35543822461816 27.046766367585356 23.312002547471586 122-123 22.34367556812279 27.305918654009513 27.022416127286007 23.32798965058169 124-125 22.307143271938052 27.21201487719845 26.952849460298594 23.527992390564904 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 373.0 1 426.5 2 536.0 3 567.5 4 498.0 5 446.0 6 475.0 7 582.0 8 759.0 9 886.0 10 895.0 11 926.5 12 1044.5 13 1141.5 14 1256.5 15 1422.5 16 1597.0 17 1837.0 18 2105.5 19 2467.5 20 3033.5 21 3851.0 22 4949.0 23 6545.5 24 8690.5 25 11455.0 26 15365.5 27 20190.5 28 24859.0 29 29592.0 30 35302.5 31 42315.0 32 51177.0 33 57012.5 34 60525.0 35 65794.0 36 72552.0 37 78898.0 38 83126.0 39 86971.0 40 91235.0 41 95901.5 42 99198.0 43 102157.5 44 106336.5 45 110651.0 46 112767.0 47 113402.5 48 113534.5 49 112780.0 50 111573.5 51 110688.5 52 110245.0 53 109677.0 54 108282.5 55 102273.0 56 94508.0 57 87970.5 58 81624.0 59 72743.0 60 60506.5 61 49492.5 62 40246.0 63 30932.0 64 22905.0 65 17442.5 66 13345.5 67 10394.0 68 8241.0 69 6469.0 70 4857.0 71 3544.0 72 2663.0 73 2119.0 74 1687.0 75 1252.0 76 905.5 77 636.0 78 421.5 79 278.5 80 202.0 81 143.5 82 96.0 83 73.0 84 60.0 85 47.0 86 37.0 87 25.0 88 22.5 89 17.5 90 8.0 91 12.5 92 15.0 93 9.5 94 8.0 95 8.0 96 7.0 97 5.0 98 4.5 99 4.5 100 8.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.010936108360108037 2 0.004277948270277556 3 0.0069476453111274586 4 0.007365790630778647 5 0.013155495056718198 6 9.006206884794853E-4 7 0.008169916245492473 8 0.0 9 0.0022193866966101605 10-11 0.0 12-13 0.0036668128030950473 14-15 0.019813655146548675 16-17 0.031007083703365138 18-19 0.005902282011999484 20-21 0.00823424629466958 22-23 0.0014635086187791636 24-25 0.01202971919611884 26-27 0.01949200490066315 28-29 6.915480286538905E-4 30-31 0.001688663790899035 32-33 0.009086619446266236 34-35 0.009327857130680383 36-37 0.011354253679759225 38-39 0.0 40-41 0.025249544302014138 42-43 0.009842497524097231 44-45 0.016323749978690672 46-47 0.004342278319454661 48-49 0.025683772133959608 50-51 0.004036710585863408 52-53 0.0 54-55 0.018092826331061088 56-57 0.004615681028457362 58-59 2.894852212969774E-4 60-61 0.004551350979280256 62-63 0.02734027090027009 64-65 0.017272618204052986 66-67 0.0019138189630189062 68-69 0.021486236425153436 70-71 0.003103924872795369 72-73 0.01230312190512154 74-75 1.6082512294276522E-5 76-77 0.006416922405416333 78-79 0.001045363299127974 80-81 0.0034738226555637293 82-83 0.0016082512294276522 84-85 0.0 86-87 0.006159602208707909 88-89 0.017176123130287328 90-91 0.022419022138221473 92-93 0.005017743835814275 94-95 0.025732019670842435 96-97 0.018414476576946618 98-99 8.362906393023793E-4 100-101 0.0089740418602063 102-103 0.0 104-105 0.0013026834958363982 106-107 8.041256147138261E-5 108-109 0.0 110-111 6.593830040653375E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 6.433004917710609E-5 122-123 0.0 124-125 0.02317490021605247 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3108967.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.58079078741706 #Duplication Level Percentage of deduplicated Percentage of total 1 81.48037242236934 52.620668846898596 2 10.47991863059578 13.536028651033206 3 3.5176862505433752 6.815248794063457 4 1.578144388064859 4.076712502318118 5 0.8200029577450543 2.6478219729598274 6 0.5120864854110102 1.9842570107635287 7 0.3350123280338695 1.51447527475726 8 0.21775228676000175 1.1250091899783452 9 0.1725825987089863 1.0030968630696417 >10 0.8206265289632524 9.377762881580392 >50 0.04603740705135101 2.0283827093464453 >100 0.018016337314104014 2.0319471011817782 >500 0.001056827063506877 0.49686529609296193 >1k 7.045513755826428E-4 0.7417229059563161 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.649507376565914E-5 2 0.0 0.0 0.0 0.0 9.649507376565914E-5 3 0.0 0.0 0.0 0.0 9.649507376565914E-5 4 0.0 0.0 0.0 0.0 9.649507376565914E-5 5 3.2165024588553044E-5 0.0 0.0 0.0 9.649507376565914E-5 6 3.2165024588553044E-5 0.0 0.0 0.0 9.649507376565914E-5 7 3.2165024588553044E-5 0.0 0.0 0.0 9.649507376565914E-5 8 3.2165024588553044E-5 0.0 0.0 0.0 9.649507376565914E-5 9 3.2165024588553044E-5 0.0 0.0 0.0 9.649507376565914E-5 10-11 3.2165024588553044E-5 0.0 0.0 0.0 9.649507376565914E-5 12-13 3.2165024588553044E-5 0.0 0.0 1.6082512294276522E-5 2.5732019670842435E-4 14-15 3.2165024588553044E-5 0.0 0.0 6.433004917710609E-5 2.894852212969774E-4 16-17 3.2165024588553044E-5 0.0 0.0 1.1257758605993565E-4 3.8598029506263656E-4 18-19 3.2165024588553044E-5 0.0 0.0 1.7690763523704175E-4 4.663928565340192E-4 20-21 3.2165024588553044E-5 0.0 0.0 2.734027090027009E-4 5.789704425939548E-4 22-23 3.2165024588553044E-5 0.0 0.0 3.37732758179807E-4 6.915480286538905E-4 24-25 3.2165024588553044E-5 0.0 0.0 5.146403934168487E-4 7.719605901252731E-4 26-27 3.2165024588553044E-5 0.0 0.0 6.433004917710609E-4 8.202081270081027E-4 28-29 3.2165024588553044E-5 0.0 0.0 0.0011257758605993566 8.362906393023792E-4 30-31 3.2165024588553044E-5 1.6082512294276522E-5 0.0 0.0028144396514983914 8.362906393023792E-4 32-33 3.2165024588553044E-5 3.2165024588553044E-5 0.0 0.00601485959805942 9.006206884794852E-4 34-35 3.2165024588553044E-5 3.2165024588553044E-5 0.0 0.009729919938037297 9.649507376565914E-4 36-37 3.2165024588553044E-5 3.2165024588553044E-5 0.0 0.016918802933578902 9.649507376565914E-4 38-39 3.2165024588553044E-5 3.2165024588553044E-5 0.0 0.02960790513376308 9.649507376565914E-4 40-41 3.2165024588553044E-5 3.2165024588553044E-5 0.0 0.045368767182154074 9.649507376565914E-4 42-43 3.2165024588553044E-5 3.2165024588553044E-5 0.0 0.06177292972231613 9.810332499508678E-4 44-45 3.2165024588553044E-5 3.2165024588553044E-5 0.0 0.08068596418038532 0.001013198274539421 46-47 3.2165024588553044E-5 3.2165024588553044E-5 0.0 0.1025581809006014 0.0010936108360108036 48-49 3.2165024588553044E-5 3.2165024588553044E-5 0.0 0.1283706131329152 0.0011579408851879096 50-51 3.2165024588553044E-5 3.2165024588553044E-5 0.0 0.15821975595109244 0.0012222709343650158 52-53 3.2165024588553044E-5 3.2165024588553044E-5 0.0 0.18710394803161307 0.0013026834958363982 54-55 3.2165024588553044E-5 3.2165024588553044E-5 0.0 0.21672793567767043 0.0013991785696020574 56-57 3.2165024588553044E-5 3.2165024588553044E-5 0.0 0.25468266469216305 0.0014313435941906106 58-59 3.2165024588553044E-5 3.2165024588553044E-5 0.0 0.2981536954235925 0.001447426106484887 60-61 3.2165024588553044E-5 3.2165024588553044E-5 0.0 0.3487010315645036 0.0014635086187791636 62-63 3.2165024588553044E-5 3.2165024588553044E-5 0.0 0.4008244539102538 0.0015439211802505462 64-65 3.2165024588553044E-5 3.2165024588553044E-5 0.0 0.45269055605929553 0.0015760862048390992 66-67 3.2165024588553044E-5 3.2165024588553044E-5 0.0 0.5160556544987451 0.0015921687171333758 68-69 3.2165024588553044E-5 3.2165024588553044E-5 0.0 0.5893597455360575 0.0016082512294276524 70-71 3.2165024588553044E-5 3.2165024588553044E-5 0.0 0.6702226173516799 0.001624333741721929 72-73 3.2165024588553044E-5 3.2165024588553044E-5 0.0 0.7579044743800754 0.0018816539384303532 74-75 3.2165024588553044E-5 3.2165024588553044E-5 0.0 0.8461974668756536 0.002138974135138778 76-77 3.2165024588553044E-5 3.2165024588553044E-5 0.0 0.942708623153607 0.0021872216720216072 78-79 3.2165024588553044E-5 3.2165024588553044E-5 0.0 1.0573608532995042 0.0022033041843158834 80-81 3.2165024588553044E-5 3.2165024588553044E-5 0.0 1.1898485895797544 0.0022354692089044366 82-83 4.824753688282956E-5 3.2165024588553044E-5 0.0 1.3297503640276658 0.0024606243810243083 84-85 6.433004917710609E-5 3.2165024588553044E-5 0.0 1.4753775128523396 0.0024767068933185844 86-87 6.433004917710609E-5 3.2165024588553044E-5 0.0 1.6300108685618084 0.0025732019670842436 88-89 6.433004917710609E-5 3.2165024588553044E-5 0.0 1.802608390503984 0.002669697040849903 90-91 6.433004917710609E-5 3.2165024588553044E-5 0.0 1.9992814333506916 0.002669697040849903 92-93 6.433004917710609E-5 3.2165024588553044E-5 0.0 2.212439051299033 0.0027501096023212854 94-95 6.433004917710609E-5 3.2165024588553044E-5 0.0 2.429440389685706 0.0028466046760869446 96-97 6.433004917710609E-5 3.2165024588553044E-5 0.0 2.6513147292975447 0.003071759848206816 98-99 6.433004917710609E-5 3.2165024588553044E-5 0.0 2.8925845787362814 0.003216502458855305 100-101 6.433004917710609E-5 3.2165024588553044E-5 0.0 3.17504174216066 0.003216502458855305 102-103 6.433004917710609E-5 4.824753688282956E-5 0.0 3.481027621071565 0.0032647499957381343 104-105 6.433004917710609E-5 6.433004917710609E-5 0.0 3.7961162019410306 0.0033129975326209636 106-107 6.433004917710609E-5 6.433004917710609E-5 0.0 4.111928495863738 0.0033129975326209636 108-109 6.433004917710609E-5 6.433004917710609E-5 0.0 4.443517734347132 0.0033290800449152403 110-111 6.433004917710609E-5 6.433004917710609E-5 0.0 4.825332658725551 0.00337732758179807 112-113 6.433004917710609E-5 6.433004917710609E-5 0.0 5.242368285028435 0.00337732758179807 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3195 0.0 54.190895 1 GTATCAA 5725 0.0 52.275314 1 ATCAACG 6530 0.0 45.100586 3 TCAACGC 6685 0.0 44.143875 4 CAACGCA 6845 0.0 43.285866 5 AACGCAG 7210 0.0 40.594868 6 TATCAAC 7300 0.0 40.58791 2 GTACATG 9565 0.0 33.90125 1 ACGCAGA 8475 0.0 33.76344 7 CGCAGAG 8280 0.0 33.269085 8 TACATGG 9805 0.0 32.22086 2 ACATGGG 9810 0.0 30.627573 3 GCAGAGT 10125 0.0 25.91396 9 GTATAGG 980 0.0 23.677874 1 CATGGGG 6155 0.0 23.005894 4 AGAGTAC 8835 0.0 22.866163 10-11 GAGTACA 6995 0.0 22.540436 1 CAGAGTA 9485 0.0 22.240213 10-11 GAGTACT 5475 0.0 20.707125 12-13 AGTACAT 7110 0.0 20.41787 2 >>END_MODULE