FastQCFastQC Report
Thu 26 May 2016
SRR1364591_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364591_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3108967
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50210.16150058845912485No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43220.13901723627172627No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42150.054.5975721
GTATCAA71550.052.577911
ATCAACG91600.040.378763
TCAACGC93400.039.5368164
CAACGCA93300.039.3471345
AACGCAG96150.038.2427186
TATCAAC96700.038.0027922
GTACATG97850.032.414131
ACGCAGA115250.031.8016287
CGCAGAG116300.031.3098728
TACATGG97700.031.090842
ACATGGG98000.029.9624753
GCAGAGT136600.026.0027459
GTGGTAT27450.024.542551
AGAGTAC123650.023.98532910-11
TGGTATC26400.022.560722
CATGGGG59950.022.5524394
GAGTACT91800.022.32760412-13
GAGTACA73650.021.9371471
CAGAGTA134000.021.79974210-11
Produced by FastQC (version 0.11.5)