##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364591_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3108967 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.089257943233235 31.0 30.0 34.0 26.0 34.0 2 30.012188936067833 31.0 30.0 34.0 26.0 34.0 3 30.286284801350416 31.0 30.0 34.0 26.0 34.0 4 33.824168284835444 35.0 35.0 37.0 29.0 37.0 5 33.255361668361225 35.0 33.0 37.0 27.0 37.0 6 33.292360774495194 35.0 33.0 37.0 27.0 37.0 7 33.07676955078648 35.0 33.0 37.0 26.0 37.0 8 33.01154402732483 36.0 33.0 37.0 26.0 37.0 9 34.28389879982644 38.0 34.0 39.0 25.0 39.0 10-11 34.204369329105134 38.0 34.0 39.0 25.0 39.0 12-13 34.090780635497254 38.0 34.0 39.0 25.0 39.0 14-15 35.07333464781067 39.0 34.0 41.0 25.0 41.0 16-17 35.065303845296526 39.0 34.0 41.0 25.0 41.0 18-19 35.06477183578983 39.0 34.0 41.0 25.0 41.0 20-21 35.066754327080346 39.0 34.0 41.0 25.0 41.0 22-23 35.02733721522293 39.0 34.0 41.0 25.0 41.0 24-25 34.97875580538488 39.0 34.0 41.0 24.5 41.0 26-27 34.85403318851567 39.0 34.0 41.0 24.0 41.0 28-29 34.71050593332126 39.0 34.0 41.0 24.0 41.0 30-31 34.568647721252745 38.5 33.5 41.0 23.5 41.0 32-33 34.39282436899459 38.0 33.0 40.5 23.0 41.0 34-35 34.22343321752852 38.0 33.0 40.0 22.0 41.0 36-37 34.032340002322314 38.0 33.0 40.0 20.0 41.0 38-39 33.87101423077183 38.0 33.0 40.0 18.5 41.0 40-41 33.91389792815427 38.0 33.0 40.0 18.5 41.0 42-43 33.80113893135566 38.0 33.0 40.0 17.5 41.0 44-45 33.66558667235773 38.0 33.0 40.0 16.0 41.0 46-47 33.474725849454174 38.0 32.5 40.0 14.5 41.0 48-49 33.23543077170005 38.0 32.0 40.0 11.5 41.0 50-51 33.075107583965995 38.0 32.0 40.0 9.0 41.0 52-53 32.88455184632066 38.0 32.0 40.0 8.0 41.0 54-55 32.699289989247234 38.0 31.5 40.0 7.5 41.0 56-57 32.48002182075268 37.0 31.0 40.0 7.0 41.0 58-59 32.27289112428662 37.0 31.0 40.0 3.5 41.0 60-61 32.03275991671832 36.5 31.0 40.0 2.0 41.0 62-63 31.857818851084623 36.0 31.0 40.0 2.0 41.0 64-65 31.72301265983203 36.0 31.0 39.0 2.0 41.0 66-67 31.427128689368526 35.5 30.5 39.0 2.0 41.0 68-69 31.132820805109866 35.0 30.0 39.0 2.0 41.0 70-71 30.813995613333947 35.0 30.0 38.5 2.0 40.0 72-73 30.523574068171197 35.0 30.0 37.5 2.0 40.0 74-75 30.172646091129305 35.0 30.0 37.0 2.0 39.0 76-77 29.862217739847353 35.0 29.0 36.5 2.0 39.0 78-79 29.575300734938647 34.5 29.0 36.0 2.0 39.0 80-81 29.297123771336267 34.0 29.0 36.0 2.0 37.5 82-83 29.006662180717903 34.0 29.0 35.0 2.0 37.0 84-85 28.785197784344447 34.0 29.0 35.0 2.0 36.5 86-87 28.570773507727807 34.0 29.0 35.0 2.0 36.0 88-89 28.391031972999393 34.0 29.0 35.0 2.0 36.0 90-91 28.24913934435457 34.0 29.0 35.0 2.0 36.0 92-93 28.137266815633616 34.0 29.0 35.0 2.0 35.0 94-95 28.02162374190527 34.0 28.0 35.0 2.0 35.0 96-97 27.906254231711046 34.0 28.0 35.0 2.0 35.0 98-99 27.821968197153588 34.0 27.5 35.0 2.0 35.0 100-101 27.72024373369032 34.0 27.0 35.0 2.0 35.0 102-103 26.43446520982693 31.5 24.5 34.0 2.0 35.0 104-105 27.385548157957288 33.5 26.5 35.0 2.0 35.0 106-107 27.55480791529791 34.0 27.0 35.0 2.0 35.0 108-109 27.561002738208543 34.0 27.0 35.0 2.0 35.0 110-111 27.47569626824601 34.0 27.0 35.0 2.0 35.0 112-113 27.389708864712944 34.0 27.0 35.0 2.0 35.0 114-115 27.290931039152234 34.0 27.0 35.0 2.0 35.0 116-117 27.169660051071624 34.0 27.0 35.0 2.0 35.0 118-119 27.029021215085265 34.0 27.0 35.0 2.0 35.0 120-121 26.83337954375199 34.0 25.5 35.0 2.0 35.0 122-123 26.5408624472373 34.0 25.0 35.0 2.0 35.0 124-125 24.73608436499969 31.5 21.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6162163349904581 1101 2 0.7491793405248686 1101 3 0.5384022801269062 1101 4 0.526263551621355 1101 5 0.602219576395072 1101 6 0.6251062932757989 1101 7 0.7154484534826437 1101 8 0.8119261064310805 1101 9 0.886419930438386 1101 10-11 0.9627444108777397 1101 12-13 0.9040076536266 1101 14-15 1.0102665042280066 1101 16-17 1.100547088455059 1101 18-19 1.150768756254621 1101 20-21 1.1111379909440373 1101 22-23 1.0598261061689271 1101 24-25 1.0675651972664326 1101 26-27 0.9825993358251992 1101 28-29 1.14746869898989 1101 30-31 1.141135202649835 1101 32-33 1.1428871748780836 1101 34-35 1.162815530353818 1101 36-37 1.2631060414065018 1101 38-39 1.141361301323549 1101 40-41 0.9739069411808856 1101 42-43 1.00855722695092 1101 44-45 1.0085970981583046 1101 46-47 1.1333723360867793 1101 48-49 1.1137802058387365 1101 50-51 1.1691675950909683 1101 52-53 1.1916095785915246 1101 54-55 1.2936735452181694 1101 56-57 1.1528419035708382 1101 58-59 1.1794776707966435 1101 60-61 1.1360407098167542 1101 62-63 1.200619368087704 1101 64-65 1.1243067143964716 1101 66-67 1.241527664198216 1101 68-69 1.280309216395107 1101 70-71 1.3078620492666886 1101 72-73 1.1891074053457054 1101 74-75 1.2413187996620394 1101 76-77 1.2350162866901684 1101 78-79 1.1438871799606893 1101 80-81 1.0266960329605723 1101 82-83 1.2369582340118335 1101 84-85 1.233616260976941 1101 86-87 1.2540929166857673 1101 88-89 1.2116812442702773 1101 90-91 1.1884469025866586 1101 92-93 1.1930392458519385 1101 94-95 1.1822706250633885 1101 96-97 1.3132284429219148 1101 98-99 1.2928095000896676 1101 100-101 1.3041646249668162 1101 102-103 1.1562496781388418 1101 104-105 1.2931965149179412 1101 106-107 1.2181126049024478 1101 108-109 1.2406742841228144 1101 110-111 1.1305834320964898 1101 112-113 1.1547092819246068 1101 114-115 1.2662294864262122 1101 116-117 1.3124951186933984 1101 118-119 1.2211964400761097 1101 120-121 1.2727301405573428 1101 122-123 1.2328177374312865 1101 124-125 1.5038979506313304 1102 1 0.4465620521133502 1102 2 -0.28701452626089363 1102 3 0.3048824087112685 1102 4 0.3913522632818456 1102 5 0.6136368249325344 1102 6 0.5669925415710466 1102 7 0.4841307165186848 1102 8 0.5859268815824095 1102 9 1.00914213940937 1102 10-11 0.9637531959876071 1102 12-13 1.0185718191666098 1102 14-15 1.2078399780404467 1102 16-17 1.158330635287058 1102 18-19 1.1629992034682317 1102 20-21 0.9245073959314638 1102 22-23 1.1736183025238702 1102 24-25 1.051557086401715 1102 26-27 1.2116184594836028 1102 28-29 0.9159358215606446 1102 30-31 1.0485690920370985 1102 32-33 1.2128394169230532 1102 34-35 1.221792979943615 1102 36-37 0.979177352377036 1102 38-39 0.32217194321784604 1102 40-41 -0.08170283376881571 1102 42-43 0.31244237321045887 1102 44-45 0.04825202853211863 1102 46-47 0.2802758260571352 1102 48-49 -0.055026151508968724 1102 50-51 -0.13112067007350703 1102 52-53 -0.40758991440564074 1102 54-55 -0.2618497584834216 1102 56-57 -0.23646927246211646 1102 58-59 -0.19624087068658724 1102 60-61 -0.22477696783946044 1102 62-63 -0.17657301240718581 1102 64-65 -0.25489295200436857 1102 66-67 -0.35111324980887204 1102 68-69 -0.08550728340741642 1102 70-71 -0.6231232876077648 1102 72-73 -0.4892749855478691 1102 74-75 -0.30941173711272896 1102 76-77 -0.6023672676682885 1102 78-79 -0.459806650391851 1102 80-81 -0.5642716739252336 1102 82-83 -0.8185078833710158 1102 84-85 -0.6906435497511012 1102 86-87 -0.502764921999745 1102 88-89 -0.6511577852815797 1102 90-91 -0.5508765500576267 1102 92-93 -0.4470665433035812 1102 94-95 -0.2524698698786665 1102 96-97 -0.5329858781187546 1102 98-99 -0.6261853876379071 1102 100-101 -0.4535569116037408 1102 102-103 -0.5514902779605109 1102 104-105 -0.44067971027588015 1102 106-107 -0.3445322402028772 1102 108-109 -0.3095262184895837 1102 110-111 -0.225419710937377 1102 112-113 -0.023942807027051316 1102 114-115 -0.10064735840904504 1102 116-117 -0.3117958862491612 1102 118-119 0.0023425074006375723 1102 120-121 0.2378167861314786 1102 122-123 0.1754585684259773 1102 124-125 0.4027220246695329 1103 1 0.24623507388016463 1103 2 -0.09486637373253615 1103 3 0.223406179344515 1103 4 0.18549953142184705 1103 5 0.3747219895224916 1103 6 0.47411298351232745 1103 7 0.5239434404050485 1103 8 0.4431963455642247 1103 9 0.6777589790859011 1103 10-11 0.6939486428380235 1103 12-13 0.7042904442067695 1103 14-15 0.796619012606719 1103 16-17 0.7041766691511668 1103 18-19 0.7498738557377465 1103 20-21 0.7307740014570712 1103 22-23 0.7813374917428106 1103 24-25 0.6916524017467864 1103 26-27 0.7556780511922199 1103 28-29 0.7528822513540447 1103 30-31 0.7641888667821206 1103 32-33 0.8108408870606283 1103 34-35 0.7093494814996646 1103 36-37 0.785037327072672 1103 38-39 0.7781038906871061 1103 40-41 0.7490454394796657 1103 42-43 0.8285542423864456 1103 44-45 0.8095333261358704 1103 46-47 0.8334465609845978 1103 48-49 0.7778742852554004 1103 50-51 0.8264245981141016 1103 52-53 0.7940745245544534 1103 54-55 0.8266426773556503 1103 56-57 0.8798362100762347 1103 58-59 0.8475415128776262 1103 60-61 0.8451846404828629 1103 62-63 0.810544177231268 1103 64-65 0.8006109708418627 1103 66-67 0.7318353485583131 1103 68-69 0.8283223580322385 1103 70-71 0.7808711130015631 1103 72-73 0.7215514080518055 1103 74-75 0.8497400924331302 1103 76-77 0.8713017706319022 1103 78-79 0.717793629875203 1103 80-81 0.7447783096365974 1103 82-83 0.8793805185712138 1103 84-85 0.87372745413753 1103 86-87 0.948191643672601 1103 88-89 0.9663978999445959 1103 90-91 0.87947247466062 1103 92-93 0.7996613964509045 1103 94-95 0.8160701808639494 1103 96-97 0.8208678311056588 1103 98-99 0.8509428717311067 1103 100-101 0.742608690301001 1103 102-103 0.5917291262191 1103 104-105 0.5523295122061818 1103 106-107 0.729914859018205 1103 108-109 0.7778460881797002 1103 110-111 0.707409031981566 1103 112-113 0.6530322556760062 1103 114-115 0.7617221217877983 1103 116-117 0.7303954908528425 1103 118-119 0.6230994051091123 1103 120-121 0.7187471623052097 1103 122-123 0.7791794613383729 1103 124-125 1.0503856383740562 1104 1 0.3324352286086345 1104 2 0.4983241108170411 1104 3 0.2410442962261783 1104 4 0.06012497360329405 1104 5 0.3316770096484021 1104 6 0.5543407980568062 1104 7 0.6106682914228401 1104 8 0.4905814134261135 1104 9 0.530189286454565 1104 10-11 0.5527633390292337 1104 12-13 0.555496496731763 1104 14-15 0.6244474584685733 1104 16-17 0.6281559290193144 1104 18-19 0.4568583298081137 1104 20-21 0.38674952135313134 1104 22-23 0.5135856322682173 1104 24-25 0.6456210765899542 1104 26-27 0.6307115564227175 1104 28-29 0.7337594147845934 1104 30-31 0.7072534942985627 1104 32-33 0.804312776128441 1104 34-35 0.8536659476430017 1104 36-37 0.8387005962651699 1104 38-39 0.9262071705382127 1104 40-41 0.9035643140052514 1104 42-43 0.9086337798618445 1104 44-45 0.8159339857991128 1104 46-47 0.8373733520997675 1104 48-49 0.9004916910868701 1104 50-51 0.693132644835373 1104 52-53 0.7621486824475738 1104 54-55 0.5387353632470493 1104 56-57 0.725934313566313 1104 58-59 0.8331874998532385 1104 60-61 0.8170346845608591 1104 62-63 0.8766235650937695 1104 64-65 0.8830845555809255 1104 66-67 1.0022533922242616 1104 68-69 0.6513867323980485 1104 70-71 0.2415565573133307 1104 72-73 0.2972621043579444 1104 74-75 0.7194833407569057 1104 76-77 0.8131363620099918 1104 78-79 0.843837591540666 1104 80-81 0.7893469891398404 1104 82-83 0.8070132500892946 1104 84-85 0.908928596766625 1104 86-87 0.9184215456148337 1104 88-89 0.8387574687931583 1104 90-91 0.8782892363507493 1104 92-93 0.8671664866180784 1104 94-95 0.8517145560846906 1104 96-97 0.8861933950797116 1104 98-99 0.8960119179085808 1104 100-101 0.8817001799899771 1104 102-103 0.7452886343115068 1104 104-105 0.8559732257314643 1104 106-107 0.841145676507292 1104 108-109 0.8175711595874482 1104 110-111 0.8257970046812382 1104 112-113 0.7787406175654361 1104 114-115 0.7753223985191013 1104 116-117 0.8164401418938958 1104 118-119 0.8331770032229393 1104 120-121 0.7286012695822137 1104 122-123 0.7700602354502273 1104 124-125 1.1121044409312795 1105 1 0.06243200116794512 1105 2 -0.2389047308930934 1105 3 0.009227004206678657 1105 4 0.09103834018268486 1105 5 -0.19366051767214287 1105 6 0.24191027588179992 1105 7 0.39230653547553374 1105 8 0.3124777766619218 1105 9 0.4816560906040692 1105 10-11 0.4481027582083428 1105 12-13 0.4035718040269458 1105 14-15 0.522266608460022 1105 16-17 0.4171824491419329 1105 18-19 0.49188124800837585 1105 20-21 0.4192920820115731 1105 22-23 0.5583979136071662 1105 24-25 0.3513884443767239 1105 26-27 0.44191366721301506 1105 28-29 0.5367084276618925 1105 30-31 0.5937401651241174 1105 32-33 0.6229997009393671 1105 34-35 0.6998456143862981 1105 36-37 0.6855944752749963 1105 38-39 0.7462991580006744 1105 40-41 0.5997862460877457 1105 42-43 0.5169819720866045 1105 44-45 0.5344503494247803 1105 46-47 0.41662603900789463 1105 48-49 0.5052886395972394 1105 50-51 0.35022653742578314 1105 52-53 0.47837244803795187 1105 54-55 0.5037664086158244 1105 56-57 0.3864342863576127 1105 58-59 0.1118278374849524 1105 60-61 0.18231053910757566 1105 62-63 0.37064329082052794 1105 64-65 0.2489767445390818 1105 66-67 0.49970409444006236 1105 68-69 0.5687807402332048 1105 70-71 0.5688030723624635 1105 72-73 0.5852597291828978 1105 74-75 0.5243954216331908 1105 76-77 0.5594385824606576 1105 78-79 0.42423602637672886 1105 80-81 0.35778232528878817 1105 82-83 0.3440101500259658 1105 84-85 0.3419301351929853 1105 86-87 0.45905453547015895 1105 88-89 0.3960208981902902 1105 90-91 0.2348495098537633 1105 92-93 0.31941391474722636 1105 94-95 0.2912770660610349 1105 96-97 0.3458997035959861 1105 98-99 0.2549535256961768 1105 100-101 0.28308963491643624 1105 102-103 0.03325384209458804 1105 104-105 0.1825602700921145 1105 106-107 0.1763665893547639 1105 108-109 0.25888728024955654 1105 110-111 0.23219642698362009 1105 112-113 0.20006171229844583 1105 114-115 0.11361091442759275 1105 116-117 -0.15186370641094626 1105 118-119 -0.011808851925007247 1105 120-121 0.10425252255058837 1105 122-123 0.10234796790365053 1105 124-125 0.6144355504123205 1106 1 -0.1289023516809884 1106 2 -0.5413627376812329 1106 3 0.030204450072872646 1106 4 -0.0831273581796168 1106 5 0.1791868887437431 1106 6 0.3909089638146668 1106 7 0.4708885847018891 1106 8 0.467231503603621 1106 9 0.5479956087240154 1106 10-11 0.4442514085274425 1106 12-13 0.4218462017651845 1106 14-15 0.3864760041321418 1106 16-17 0.42049380726385266 1106 18-19 0.5048752156781973 1106 20-21 0.49304138006448994 1106 22-23 0.44024653319952733 1106 24-25 0.4886655430660696 1106 26-27 0.5249512703859907 1106 28-29 0.6125159979184076 1106 30-31 0.5000905788755716 1106 32-33 0.47975801658422057 1106 34-35 0.5544791052971831 1106 36-37 0.541210383073583 1106 38-39 0.42946955720108093 1106 40-41 0.43490170093179614 1106 42-43 0.2563009642142049 1106 44-45 0.37640839771167833 1106 46-47 0.3696556911953195 1106 48-49 0.540302709442301 1106 50-51 0.5517252165291211 1106 52-53 0.48545321786640727 1106 54-55 0.4811716369296306 1106 56-57 0.04605349992964136 1106 58-59 0.4693266852321827 1106 60-61 0.5464293438726955 1106 62-63 0.39347513520194255 1106 64-65 0.46619875141904643 1106 66-67 0.2367955524062353 1106 68-69 0.3992436103226673 1106 70-71 0.4706644020533268 1106 72-73 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0.04229292939026763 2211 66-67 -0.018039296538578498 2211 68-69 -0.05164413948887514 2211 70-71 0.011745439229581933 2211 72-73 -0.006273352082647676 2211 74-75 0.05536201337676516 2211 76-77 -0.011765709739645303 2211 78-79 0.0030689245399635467 2211 80-81 0.02673393026149995 2211 82-83 0.06382253882652833 2211 84-85 0.06658599380644858 2211 86-87 0.02971463265700436 2211 88-89 -0.1893574368598081 2211 90-91 0.0328324279929042 2211 92-93 0.010202031326318917 2211 94-95 0.019873945794355308 2211 96-97 -0.0835400892165552 2211 98-99 -0.03517045658010076 2211 100-101 -0.004244158000357601 2211 102-103 0.07623262561807564 2211 104-105 0.1537000279850922 2211 106-107 -0.05011947462742228 2211 108-109 -0.009928953225664117 2211 110-111 -0.1261688425635903 2211 112-113 0.06232474665385368 2211 114-115 0.042935280194242864 2211 116-117 0.14651153787818316 2211 118-119 0.06466581193277321 2211 120-121 0.0809032351936061 2211 122-123 0.0384787342289421 2211 124-125 -0.1364792796922778 2212 1 -0.08300978469682363 2212 2 0.18147876088764292 2212 3 -0.04020642920265871 2212 4 -0.02494308628610753 2212 5 -0.2081924343779491 2212 6 -0.21036927627453395 2212 7 -0.23933461653275145 2212 8 -0.2766879114364542 2212 9 -0.43464604213568236 2212 10-11 -0.38045046859290466 2212 12-13 -0.3512625281021755 2212 14-15 -0.37750917130824035 2212 16-17 -0.3819798562822072 2212 18-19 -0.4757153531688161 2212 20-21 -0.3627065786266286 2212 22-23 -0.5064516794229377 2212 24-25 -0.5674418967484698 2212 26-27 -0.6087974633566233 2212 28-29 -0.6008559775110953 2212 30-31 -0.6243492934448938 2212 32-33 -0.5810340231609672 2212 34-35 -0.6166753390112873 2212 36-37 -0.6295761667234743 2212 38-39 -0.5016229254792393 2212 40-41 -0.2299631409645002 2212 42-43 -0.1740923149335174 2212 44-45 -0.14865691227493016 2212 46-47 -0.2976551945389758 2212 48-49 -0.27889141546337015 2212 50-51 -0.20712847210413088 2212 52-53 -0.24079634021826024 2212 54-55 -0.2564671839722834 2212 56-57 -0.17013373109688246 2212 58-59 -0.20410940191352012 2212 60-61 -0.2576627070903399 2212 62-63 -0.25574807113171616 2212 64-65 -0.3275802280284914 2212 66-67 -0.42464079139029565 2212 68-69 -0.36426786587849236 2212 70-71 -0.24463882834772832 2212 72-73 -0.304373377293107 2212 74-75 -0.23424671759440585 2212 76-77 -0.1486632107077419 2212 78-79 -0.31841484845872614 2212 80-81 -0.22597768722217637 2212 82-83 -0.266364391501309 2212 84-85 -0.3535178275837225 2212 86-87 -0.3458148938893295 2212 88-89 -0.5845886969862697 2212 90-91 -0.5337636405159252 2212 92-93 -0.3728085548858111 2212 94-95 -0.2684125278149452 2212 96-97 -0.18685700790302562 2212 98-99 -0.2501886072207711 2212 100-101 -0.20183571179513748 2212 102-103 -0.17300012243388707 2212 104-105 -0.33620170289881557 2212 106-107 -0.5263711116320451 2212 108-109 -0.38345581051502364 2212 110-111 -0.49642144843116043 2212 112-113 -0.4936018417562593 2212 114-115 -0.3255298695988955 2212 116-117 -0.34031351632672013 2212 118-119 -0.2754966045964373 2212 120-121 -0.4121684300696522 2212 122-123 -0.412825085514438 2212 124-125 -0.8278579453495567 2213 1 -0.14759876294530372 2213 2 0.1384219196041343 2213 3 -0.12468181263730216 2213 4 -0.16983701402975981 2213 5 -0.11644948118611609 2213 6 -0.435859105656931 2213 7 -0.3051988930521361 2213 8 -0.31426533383783095 2213 9 -0.37353710087780456 2213 10-11 -0.43221471511795784 2213 12-13 -0.3960318151603346 2213 14-15 -0.4545935670904342 2213 16-17 -0.6425841448232958 2213 18-19 -0.43717929919917253 2213 20-21 -0.5239987825717307 2213 22-23 -0.5367206525117254 2213 24-25 -0.6490189676575895 2213 26-27 -0.4903544661203654 2213 28-29 -0.528976352337061 2213 30-31 -0.5502172766969835 2213 32-33 -0.7030406746602367 2213 34-35 -0.6517825453456254 2213 36-37 -0.6581500768397603 2213 38-39 -0.7206533655230771 2213 40-41 -0.5834891764460366 2213 42-43 -0.49914999586630415 2213 44-45 -0.5170885807194381 2213 46-47 -0.6578453767802728 2213 48-49 -0.6335360042929636 2213 50-51 -0.3924506018018974 2213 52-53 -0.3363611122553962 2213 54-55 -0.41815961195173657 2213 56-57 -0.404500897597039 2213 58-59 -0.5365584301429536 2213 60-61 -0.45107239528123344 2213 62-63 -0.36499946217547574 2213 64-65 -0.2273457160452672 2213 66-67 -0.20334399359016686 2213 68-69 -0.28897281978529676 2213 70-71 -0.30409082891644346 2213 72-73 -0.2770366431643829 2213 74-75 -0.2793127710437666 2213 76-77 -0.3234524412346289 2213 78-79 -0.423743360641911 2213 80-81 -0.4312727764726745 2213 82-83 -0.4346550314737492 2213 84-85 -0.5015654152224158 2213 86-87 -0.3428813961746293 2213 88-89 -0.34161009030058054 2213 90-91 -0.3964058767930432 2213 92-93 -0.3661506419585443 2213 94-95 -0.44797549539733694 2213 96-97 -0.5658239731462906 2213 98-99 -0.6093770005919055 2213 100-101 -0.3840722742297267 2213 102-103 -0.4390120808427156 2213 104-105 -0.46074744232605624 2213 106-107 -0.34943540474301216 2213 108-109 -0.4277734444207404 2213 110-111 -0.5200482729960108 2213 112-113 -0.3525164859638643 2213 114-115 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-2.036478895983059 2216 40-41 -2.149445701843696 2216 42-43 -2.041801512336729 2216 44-45 -1.9092074489231763 2216 46-47 -2.1422395696241665 2216 48-49 -2.0251411087794153 2216 50-51 -1.958859776065907 2216 52-53 -1.8926157306279094 2216 54-55 -1.940933568489708 2216 56-57 -1.8252796688488075 2216 58-59 -1.8504233284807228 2216 60-61 -1.741966451750148 2216 62-63 -2.0140196538935484 2216 64-65 -1.7917163382633632 2216 66-67 -1.631505692725682 2216 68-69 -1.628034446284211 2216 70-71 -1.6387721346149853 2216 72-73 -1.515075844200446 2216 74-75 -1.5364934940052528 2216 76-77 -1.567099881930858 2216 78-79 -1.5796403332140976 2216 80-81 -1.4830521072487315 2216 82-83 -1.3361715018958513 2216 84-85 -1.320746942892999 2216 86-87 -1.6156575893589746 2216 88-89 -1.5774383361701574 2216 90-91 -1.5694281857084853 2216 92-93 -1.5861861091250624 2216 94-95 -1.510199239345095 2216 96-97 -1.5200709535527857 2216 98-99 -1.541244218271519 2216 100-101 -1.58850211558984 2216 102-103 -1.4879347305988837 2216 104-105 -1.4728258767993303 2216 106-107 -1.5149994651967837 2216 108-109 -1.551528720488193 2216 110-111 -1.5688386583665554 2216 112-113 -1.5342480540633439 2216 114-115 -1.719436448797353 2216 116-117 -1.7323702557797098 2216 118-119 -1.7460584739471265 2216 120-121 -1.897509301986748 2216 122-123 -1.8418275058837033 2216 124-125 -2.1732874051184616 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 94685.0 3 31741.0 4 15384.0 5 12135.0 6 12853.0 7 15166.0 8 16793.0 9 18485.0 10 20333.0 11 21839.0 12 23352.0 13 25672.0 14 28161.0 15 25380.0 16 19385.0 17 16947.0 18 16163.0 19 15539.0 20 15289.0 21 15988.0 22 18239.0 23 22854.0 24 29438.0 25 38342.0 26 47504.0 27 54627.0 28 63062.0 29 74991.0 30 87400.0 31 102623.0 32 124594.0 33 155087.0 34 199565.0 35 273269.0 36 442518.0 37 667001.0 38 245734.0 39 829.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.23060738599138 19.04791187421825 13.712200359624793 25.00928038016558 2 15.576009652080577 19.981234924655038 39.42701868498443 25.015736738279948 3 19.59882494732173 23.96644930615217 28.959200917861143 27.475524828664955 4 12.954258029120938 16.708736538714156 36.26269501317474 34.074310418990166 5 13.2125236453137 38.20764260283239 34.58045711003044 13.999376641823474 6 32.91733234865472 36.964367907411045 16.63314535020796 13.485154393726276 7 28.64486266890968 31.93302475787334 20.627197313527894 18.794915259689088 8 25.970655956335197 36.38595150371175 18.94885250584314 18.69454003410991 9 25.784332678322198 16.04016014909679 19.341313339678653 38.83419383290236 10-11 25.133497536549694 26.55344525205837 27.35215478525323 20.96090242613871 12-13 25.88697467679779 23.549944402755 27.794505377509637 22.768575542937576 14-15 22.4912667867388 25.218863065178134 25.470570667500386 26.819299480582682 16-17 22.479385788074758 28.372820552985655 26.255890435369132 22.89190322357046 18-19 22.408733379769917 27.00108189409146 28.308859774978252 22.281324951160368 20-21 22.934573760702442 26.290713987150234 28.576694510665007 22.198017741482314 22-23 22.526469402859536 26.213851739178963 28.425727902547692 22.833950955413805 24-25 22.493628912754623 26.29722348291249 28.517591212772604 22.691556391560283 26-27 22.270660962306774 26.70639476070347 28.289203455681584 22.73374082130817 28-29 22.258792068233596 26.889928391005757 28.30481957511932 22.546459965641322 30-31 22.407104996611416 26.544299119289462 28.32463323026587 22.72396265383325 32-33 22.238045627373978 26.492609924775657 28.33732233246606 22.932022115384306 34-35 22.641362870689846 26.627719110559873 28.282657873177815 22.44826014557247 36-37 22.749324775721323 26.289729032183356 28.21424286587796 22.746703326217357 38-39 22.453776447289407 26.647098537874474 28.11467603226409 22.784448982572027 40-41 22.74990374616392 26.496421480189404 28.158758198462703 22.594916575183976 42-43 22.563153613402783 26.4129532413821 28.03302511734605 22.990868027869062 44-45 22.6532960948122 26.67881325211879 28.03307336488293 22.634817288186078 46-47 22.89028799598066 26.863874721089033 27.430445546704096 22.81539173622621 48-49 22.798022622948395 26.949787501765055 27.345883697060792 22.90630617822576 50-51 22.866995468428325 27.127753588774144 27.241698243354584 22.76355269944295 52-53 23.038424016723237 26.9516209081666 27.254679769839953 22.755275305270207 54-55 22.848746866724543 26.949642759154408 27.35940908990028 22.842201284220774 56-57 22.781975917086093 27.149997659807408 27.210874834759082 22.857151588347417 58-59 22.696623647813873 26.984876229797422 27.288548180461103 23.029951941927603 60-61 22.75513056265956 27.064262824275716 27.414893757315532 22.765712855749193 62-63 22.702926727752338 26.98972037979174 27.610135456568052 22.697217435887868 64-65 22.50379186226393 26.97864768626041 27.504579102886638 23.012981348589022 66-67 22.508537401651417 27.128367718280703 27.319910439705534 23.043184440362346 68-69 22.811853583521472 26.853774903368226 27.49194507371741 22.84242643939289 70-71 22.700691258543433 26.84968994524548 27.338485741405428 23.111133054805663 72-73 22.69779640633046 26.969327754202606 27.261852570323196 23.07102326914374 74-75 22.61402923869108 27.236618661381247 27.169955822399906 22.979396277527773 76-77 22.731553599636147 27.07067974668113 27.20815306177261 22.98961359191011 78-79 22.790801574928263 27.038916784899936 27.197908501441155 22.972373138730646 80-81 22.830856650456013 27.086149451416848 27.081051289280172 23.001942608846967 82-83 22.817096482529404 27.235814982918765 27.13562093132542 22.81146760322641 84-85 22.73922495800052 27.28131241019927 27.043918446223458 22.935544185576752 86-87 22.804729030575107 27.17032699285647 27.07116222204996 22.953781754518463 88-89 22.725678347793014 27.236068972227606 26.931575388015016 23.106677291964363 90-91 22.69955305082895 27.377554566766804 26.98777873611697 22.935113646287277 92-93 22.69213461605886 27.15480486512758 27.14763191237032 23.005428606443243 94-95 22.524459088822752 27.068058297177167 27.26032473165524 23.147157882344843 96-97 22.784605311134527 26.93163418546461 27.16626636478227 23.117494138618593 98-99 22.561217249101436 27.00109622670277 27.01637520803539 23.421311316160402 100-101 22.545522210547833 26.9113023938252 27.271559070274016 23.27161632535295 102-103 22.415548315565907 26.68156336172111 27.40801044205358 23.494877880659395 104-105 22.3484199092496 26.881597649637325 27.218285044518005 23.551697396595074 106-107 22.432891920879957 26.96841975585424 26.997384784401923 23.60130353886388 108-109 22.47552723317013 26.82891996888998 26.97991872538973 23.715634072550166 110-111 22.42454233921161 26.84164360966779 27.155478259303266 23.578335791817334 112-113 22.3871948463911 26.933158184052775 27.198197986662453 23.481448982893674 114-115 22.32360459277953 27.026066857576808 27.059566730685784 23.590761818957873 116-117 22.21480639710875 27.05947023561202 27.117833672727954 23.607889694551275 118-119 22.10755212261822 27.093693821774238 26.940186241925375 23.858567813682168 120-121 22.020195619634528 27.163200079512595 27.10554380003612 23.711060500816757 122-123 22.070088464604186 27.13739065765916 27.05113589941373 23.741384978322923 124-125 21.99025474767568 27.0628982620575 27.036354835869393 23.910492154397428 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 102.0 1 95.0 2 167.5 3 266.5 4 207.0 5 161.5 6 347.0 7 798.0 8 1341.5 9 1548.5 10 1282.5 11 943.5 12 875.5 13 1034.5 14 1203.5 15 1382.0 16 1587.0 17 1864.0 18 2259.5 19 2669.0 20 3213.5 21 4025.0 22 5181.0 23 6829.5 24 8822.0 25 11505.0 26 15562.0 27 20399.0 28 25056.0 29 30251.0 30 36154.5 31 43177.5 32 52021.0 33 57495.5 34 60417.5 35 65700.5 36 72908.5 37 79037.5 38 82607.0 39 86463.0 40 91218.0 41 96051.0 42 99006.0 43 101575.0 44 105704.0 45 110531.5 46 112747.0 47 113641.0 48 114348.5 49 113419.0 50 112509.0 51 111451.5 52 111367.0 53 111157.5 54 109698.0 55 103259.0 56 94737.5 57 88275.5 58 82175.5 59 72934.0 60 60140.0 61 48877.5 62 39465.5 63 29671.5 64 21312.0 65 16124.5 66 12269.5 67 9572.0 68 7421.5 69 5679.0 70 4250.0 71 3002.5 72 2217.0 73 1822.5 74 1396.5 75 1029.0 76 746.0 77 447.5 78 277.5 79 178.5 80 128.0 81 80.5 82 46.5 83 34.0 84 24.0 85 14.0 86 7.0 87 5.5 88 4.5 89 2.0 90 1.0 91 0.5 92 0.5 93 0.5 94 0.5 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0949511525854086 2 0.0 3 0.0 4 0.09723486933119585 5 0.0 6 0.0 7 0.0018334064015475236 8 0.006207849745590738 9 0.0036668128030950473 10-11 4.663928565340192E-4 12-13 0.0 14-15 8.041256147138261E-5 16-17 2.4123768441414785E-4 18-19 0.0021711391597273306 20-21 0.0012383534466592922 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 4.824753688282957E-5 52-53 0.0 54-55 0.0 56-57 0.007993008610255432 58-59 6.433004917710609E-4 60-61 0.0 62-63 0.0 64-65 2.0907265982559483E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0012383534466592922 76-77 0.0 78-79 0.0 80-81 1.1257758605993566E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0011096933483050802 90-91 1.6082512294276522E-5 92-93 0.002122891622844501 94-95 0.0 96-97 0.008732804175792152 98-99 0.0038919679752149185 100-101 0.002331964282670096 102-103 0.0 104-105 0.0 106-107 0.0014635086187791636 108-109 3.2165024588553044E-5 110-111 1.6082512294276523E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 8.202081270081026E-4 122-123 0.004921248762048616 124-125 0.08801958978657541 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3108967.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.756655469458764 #Duplication Level Percentage of deduplicated Percentage of total 1 76.73693390728816 44.320686534658705 2 12.790895796675569 14.775187233486783 3 4.46601193566952 7.738257380728653 4 2.0797391305597586 4.804751057203668 5 1.09368205941369 3.158370894934231 6 0.6789798861626003 2.352936441347138 7 0.431854751076973 1.7459740249561122 8 0.2994222747694498 1.3834903330992583 9 0.22846859435965575 1.187602370101995 >10 1.0952421199114473 11.274393248284758 >50 0.06879859288586129 2.758868648189341 >100 0.027514903977589083 2.7580419039410797 >500 0.0014513006479002313 0.6067765275849092 >1k 9.489273461557945E-4 0.972789699569883 >5k 5.581925565622321E-5 0.16187370191358066 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5021 0.16150058845912485 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4322 0.13901723627172627 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.9299014753131828E-4 2 0.0 0.0 0.0 0.0 1.9299014753131828E-4 3 0.0 0.0 0.0 0.0 1.9299014753131828E-4 4 0.0 0.0 0.0 0.0 1.9299014753131828E-4 5 0.0 0.0 0.0 3.2165024588553044E-5 1.9299014753131828E-4 6 0.0 0.0 0.0 3.2165024588553044E-5 1.9299014753131828E-4 7 0.0 0.0 0.0 3.2165024588553044E-5 1.9299014753131828E-4 8 0.0 0.0 0.0 3.2165024588553044E-5 1.9299014753131828E-4 9 0.0 0.0 0.0 3.2165024588553044E-5 2.2515517211987133E-4 10-11 0.0 0.0 0.0 3.2165024588553044E-5 2.2515517211987133E-4 12-13 0.0 0.0 0.0 6.433004917710609E-5 4.5031034423974266E-4 14-15 0.0 0.0 0.0 9.649507376565914E-5 4.824753688282957E-4 16-17 0.0 0.0 0.0 1.1257758605993565E-4 5.468054180054018E-4 18-19 0.0 0.0 0.0 1.7690763523704175E-4 6.111354671825079E-4 20-21 0.0 0.0 0.0 2.4123768441414785E-4 7.237130532424436E-4 22-23 0.0 0.0 0.0 3.055677335912539E-4 8.202081270081027E-4 24-25 0.0 0.0 0.0 4.5031034423974266E-4 8.684556638909322E-4 26-27 0.0 0.0 0.0 5.789704425939548E-4 9.327857130680383E-4 28-29 0.0 0.0 0.0 0.001141858372893633 9.327857130680383E-4 30-31 0.0 0.0 0.0 0.0027822746269098387 9.649507376565914E-4 32-33 0.0 0.0 0.0 0.006111354671825079 9.649507376565914E-4 34-35 0.0 0.0 0.0 0.009987240134745721 9.649507376565914E-4 36-37 0.0 0.0 0.0 0.017497773376172858 9.649507376565914E-4 38-39 0.0 0.0 0.0 0.030749763506656713 9.810332499508678E-4 40-41 0.0 0.0 0.0 0.046655368165696195 9.971157622451444E-4 42-43 0.0 0.0 0.0 0.06299520065668114 0.001045363299127974 44-45 0.0 0.0 0.0 0.08184390506557324 0.0011257758605993566 46-47 0.0 0.0 0.0 0.10410210208085194 0.0011740233974821862 48-49 0.0 0.0 0.0 0.12997886436234285 0.0011901059097764628 50-51 0.0 0.0 0.0 0.159474191910046 0.0012222709343650158 52-53 0.0 0.0 0.0 0.1879884862077983 0.001415261081896334 54-55 0.0 0.0 0.0 0.21724257607108727 0.0014956736433677166 56-57 0.0 0.0 0.0 0.2543449319339832 0.0015117561556619932 58-59 0.0 0.0 0.0 0.29760689000558704 0.0016082512294276524 60-61 0.0 0.0 0.0 0.3478004108760241 0.0016082512294276524 62-63 0.0 0.0 0.0 0.399730843074243 0.0017047463031933114 64-65 0.0 0.0 0.0 0.4510823048298679 0.0017047463031933114 66-67 0.0 0.0 0.0 0.5144152382447289 0.0017369113277818644 68-69 0.0 0.0 0.0 0.5880248970156325 0.0017369113277818644 70-71 0.0 3.2165024588553044E-5 0.0 0.6683409634132494 0.0017851588646646942 72-73 0.0 3.2165024588553044E-5 0.0 0.7541411665032147 0.0019459839876074594 74-75 0.0 3.2165024588553044E-5 0.0 0.8413727131873706 0.002026396549078842 76-77 0.0 3.2165024588553044E-5 0.0 0.938060777100561 0.002026396549078842 78-79 0.0 3.2165024588553044E-5 0.0 1.0524878520743384 0.002058561573667395 80-81 0.0 3.2165024588553044E-5 0.0 1.1840428026415206 0.002090726598255948 82-83 0.0 3.2165024588553044E-5 0.0 1.3237837519664892 0.0021872216720216072 84-85 0.0 3.2165024588553044E-5 0.0 1.4685424451272722 0.0022354692089044366 86-87 0.0 3.2165024588553044E-5 0.0 1.6229506456646212 0.0023158817703758192 88-89 0.0 3.2165024588553044E-5 0.0 1.795564250119091 0.0023802118195529257 90-91 0.0 3.2165024588553044E-5 0.0 1.994054616855052 0.0023802118195529257 92-93 0.0 3.2165024588553044E-5 0.0 2.207662545147633 0.0025088719179071376 94-95 0.0 3.2165024588553044E-5 0.0 2.4241492431408886 0.0025249544302014142 96-97 0.0 3.2165024588553044E-5 0.0 2.646602553195322 0.0029591822621468805 98-99 0.0 3.2165024588553044E-5 0.0 2.8893519937651315 0.003280832508032411 100-101 0.0 3.2165024588553044E-5 0.0 3.1744466892057717 0.003280832508032411 102-103 0.0 3.2165024588553044E-5 0.0 3.4830540176206437 0.003345162557209517 104-105 0.0 3.2165024588553044E-5 0.0 3.7987858989818806 0.003409492606386623 106-107 0.0 3.2165024588553044E-5 0.0 4.116045619011073 0.003409492606386623 108-109 0.0 3.2165024588553044E-5 0.0 4.451269505272974 0.0034577401432694527 110-111 0.0 3.2165024588553044E-5 0.0 4.836863820040547 0.003473822655563729 112-113 0.0 3.2165024588553044E-5 0.0 5.257920074417001 0.003505987680152282 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4215 0.0 54.597572 1 GTATCAA 7155 0.0 52.57791 1 ATCAACG 9160 0.0 40.37876 3 TCAACGC 9340 0.0 39.536816 4 CAACGCA 9330 0.0 39.347134 5 AACGCAG 9615 0.0 38.242718 6 TATCAAC 9670 0.0 38.002792 2 GTACATG 9785 0.0 32.41413 1 ACGCAGA 11525 0.0 31.801628 7 CGCAGAG 11630 0.0 31.309872 8 TACATGG 9770 0.0 31.09084 2 ACATGGG 9800 0.0 29.962475 3 GCAGAGT 13660 0.0 26.002745 9 GTGGTAT 2745 0.0 24.54255 1 AGAGTAC 12365 0.0 23.985329 10-11 TGGTATC 2640 0.0 22.56072 2 CATGGGG 5995 0.0 22.552439 4 GAGTACT 9180 0.0 22.327604 12-13 GAGTACA 7365 0.0 21.937147 1 CAGAGTA 13400 0.0 21.799742 10-11 >>END_MODULE