FastQCFastQC Report
Thu 26 May 2016
SRR1364590_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364590_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2191892
Sequences flagged as poor quality0
Sequence length125
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37130.1693970323355348No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26800.12226879791522574No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24170.1102700315526495No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36950.055.7162481
GTATCAA67250.053.793711
ATCAACG82600.042.4273153
TCAACGC82950.042.2482954
CAACGCA83900.041.5571675
AACGCAG87050.039.704386
TATCAAC89700.039.5333942
GTACATG122050.037.099421
TACATGG122800.035.8060462
ACATGGG125800.033.911643
ACGCAGA103150.033.045817
CGCAGAG102850.032.626118
CATGGGG58200.030.1584724
GCAGAGT110150.029.2215529
CGTATAG1556.8346308E-626.871171
GTATAGG7700.026.2729241
CATGGGA80000.026.1795984
GAGTACT46350.025.67909612-13
AGTACAT86150.024.448842
GAGTACA90850.024.0359521
Produced by FastQC (version 0.11.5)