##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364590_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2191892 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.381452644564604 31.0 28.0 33.0 16.0 34.0 2 28.383147983568534 31.0 28.0 34.0 16.0 34.0 3 28.582928812186 31.0 28.0 34.0 16.0 34.0 4 31.904398574382313 35.0 32.0 37.0 19.0 37.0 5 32.054305139121816 35.0 32.0 37.0 20.0 37.0 6 32.033419073567494 35.0 32.0 37.0 19.0 37.0 7 31.836281623364656 35.0 32.0 37.0 17.0 37.0 8 31.708733368249895 35.0 32.0 37.0 17.0 37.0 9 32.834928910731 37.0 32.0 39.0 16.0 39.0 10-11 32.763470782319565 37.0 32.0 39.0 15.5 39.0 12-13 32.67448350557417 37.0 32.0 39.0 14.0 39.0 14-15 33.395251225881566 38.0 32.0 41.0 11.0 41.0 16-17 33.505285388148685 38.0 32.0 41.0 11.0 41.0 18-19 33.31441535440615 38.0 32.0 41.0 10.0 41.0 20-21 33.027688179892074 38.0 31.5 41.0 10.0 41.0 22-23 32.9193155958414 38.0 31.5 40.0 10.0 41.0 24-25 32.74492812602081 38.0 31.0 40.0 10.0 41.0 26-27 32.644125486109715 38.0 31.0 40.0 9.0 41.0 28-29 32.697328609256296 38.0 31.0 40.0 8.5 41.0 30-31 32.63180439547204 38.0 31.0 40.0 8.0 41.0 32-33 32.58906164172322 38.0 31.0 40.0 8.0 41.0 34-35 32.1222701665958 38.0 30.0 40.0 4.5 41.0 36-37 31.98100294175078 38.0 30.0 40.0 2.0 41.0 38-39 31.778910411644368 37.5 30.0 40.0 2.0 41.0 40-41 31.975313792832857 38.0 30.0 40.0 2.0 41.0 42-43 31.849856425407822 37.0 30.0 40.0 2.0 41.0 44-45 31.693657123617406 37.0 30.0 40.0 2.0 41.0 46-47 31.62516446978227 37.0 30.0 40.0 2.0 41.0 48-49 31.456991037879604 37.0 30.0 40.0 2.0 41.0 50-51 31.291243136066925 37.0 30.0 40.0 2.0 41.0 52-53 31.222578028479504 37.0 29.5 40.0 2.0 41.0 54-55 31.237075093115905 37.0 30.0 40.0 2.0 41.0 56-57 31.1030187618733 36.5 29.5 40.0 2.0 41.0 58-59 30.89585846382942 36.0 29.0 40.0 2.0 41.0 60-61 30.68023766681935 36.0 29.0 40.0 2.0 41.0 62-63 30.38708727437301 35.0 29.0 39.0 2.0 41.0 64-65 30.08876760351331 35.0 28.0 39.0 2.0 41.0 66-67 29.842791296286496 35.0 28.0 39.0 2.0 40.5 68-69 29.802738456091816 35.0 29.0 39.0 2.0 40.0 70-71 29.56969983010112 35.0 29.0 38.0 2.0 40.0 72-73 29.295667396021337 35.0 29.0 37.0 2.0 40.0 74-75 28.997031331835693 35.0 28.0 37.0 2.0 39.0 76-77 28.69618165493555 34.0 28.0 36.0 2.0 39.0 78-79 28.394552970675562 34.0 28.0 36.0 2.0 38.5 80-81 28.11123130154223 34.0 27.5 35.5 2.0 37.0 82-83 27.874090283645362 34.0 27.0 35.0 2.0 37.0 84-85 27.645630122287045 34.0 27.0 35.0 2.0 36.5 86-87 27.45050782611552 34.0 26.5 35.0 2.0 36.0 88-89 27.26897333445261 34.0 26.0 35.0 2.0 36.0 90-91 27.13923929646169 34.0 26.0 35.0 2.0 36.0 92-93 27.022422409498276 34.0 26.0 35.0 2.0 35.0 94-95 26.902021404339266 34.0 26.0 35.0 2.0 35.0 96-97 26.79939431322346 34.0 25.5 35.0 2.0 35.0 98-99 26.713024409961804 34.0 25.0 35.0 2.0 35.0 100-101 26.62255827385656 34.0 25.0 35.0 2.0 35.0 102-103 25.480502917114528 31.5 23.5 34.0 2.0 35.0 104-105 26.34233780678975 33.0 25.0 35.0 2.0 35.0 106-107 26.497140826281587 34.0 25.0 35.0 2.0 35.0 108-109 26.456226629779202 34.0 25.0 35.0 2.0 35.0 110-111 26.37940122049809 34.0 25.0 35.0 2.0 35.0 112-113 26.29450994848286 34.0 25.0 35.0 2.0 35.0 114-115 26.172568721451604 34.0 24.0 35.0 2.0 35.0 116-117 26.07202864009723 33.5 24.0 35.0 2.0 35.0 118-119 25.93229433749473 33.0 23.5 35.0 2.0 35.0 120-121 25.766139937551667 33.0 23.0 35.0 2.0 35.0 122-123 25.533275590220686 33.0 19.5 35.0 2.0 35.0 124-125 24.112678909362323 31.0 9.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.9564512009700543 1101 2 0.9915535454067168 1101 3 1.0311213592489352 1101 4 1.0866545903396627 1101 5 1.0100617727807517 1101 6 1.1203572687124108 1101 7 1.1288436744713763 1101 8 1.2019704300182603 1101 9 1.5517760795318054 1101 10-11 1.4640939781050903 1101 12-13 1.273906095017793 1101 14-15 1.5809790001106663 1101 16-17 1.3496798416678928 1101 18-19 1.7754517925267237 1101 20-21 2.168744481493249 1101 22-23 1.9768703900005846 1101 24-25 2.1154089576499615 1101 26-27 2.2646242454489993 1101 28-29 2.002350826295128 1101 30-31 2.104902719397103 1101 32-33 2.0400392489475863 1101 34-35 2.221374958140437 1101 36-37 2.1578636185956164 1101 38-39 1.998086105631593 1101 40-41 1.8432457310233943 1101 42-43 1.8757935253292217 1101 44-45 1.8084220382736724 1101 46-47 1.5622274856791734 1101 48-49 1.5419399495507804 1101 50-51 1.6153238769905478 1101 52-53 1.4548421832822527 1101 54-55 1.3643392825124998 1101 56-57 1.4370278699373138 1101 58-59 1.3647506520354256 1101 60-61 1.4519140329574185 1101 62-63 1.4441853040217012 1101 64-65 1.4305959579395306 1101 66-67 1.2999898710722455 1101 68-69 1.2611521215932164 1101 70-71 1.2318022923297818 1101 72-73 1.221131299669473 1101 74-75 1.1739663233135431 1101 76-77 1.3300772827302048 1101 78-79 1.2853621893050864 1101 80-81 1.3493521886851099 1101 82-83 1.1638221529067252 1101 84-85 1.2569213716859906 1101 86-87 1.3357021649414271 1101 88-89 1.1624248669262194 1101 90-91 1.1999905462108238 1101 92-93 1.0046525926314978 1101 94-95 1.1975921445182607 1101 96-97 1.1153155975604392 1101 98-99 1.2145561713048387 1101 100-101 1.1707615722565166 1101 102-103 1.1171067756596678 1101 104-105 1.2356565667466342 1101 106-107 1.2022627221051536 1101 108-109 1.2333416082351718 1101 110-111 0.9488002245380507 1101 112-113 1.0157358382750026 1101 114-115 1.2045487068720497 1101 116-117 1.2970361525959113 1101 118-119 1.160449528294759 1101 120-121 1.321438488920343 1101 122-123 1.3239593074103624 1101 124-125 1.5838603591438485 1102 1 0.34646331741895153 1102 2 0.4841919840221678 1102 3 0.655345251576275 1102 4 0.7756458141010043 1102 5 0.7502654601108745 1102 6 0.10851016519863776 1102 7 -0.3974801294386374 1102 8 0.39067102274184506 1102 9 0.7121184510810679 1102 10-11 0.7137939322590654 1102 12-13 0.9035304032340576 1102 14-15 1.1918846227979003 1102 16-17 0.965338305171187 1102 18-19 1.2259966233876511 1102 20-21 1.6236000665212487 1102 22-23 1.4223602875019807 1102 24-25 1.5287812601027255 1102 26-27 1.1652022328085962 1102 28-29 1.5884140446859334 1102 30-31 1.543155904058338 1102 32-33 1.3224712167243808 1102 34-35 1.7937052459879723 1102 36-37 1.8682366906066115 1102 38-39 1.8881334252766138 1102 40-41 1.5008234236585594 1102 42-43 1.389183020040555 1102 44-45 1.555710982832732 1102 46-47 1.0793513845559417 1102 48-49 0.8560347362030285 1102 50-51 1.2281059118990179 1102 52-53 1.1075540574021687 1102 54-55 0.9246873848146215 1102 56-57 0.9800875188877676 1102 58-59 1.0678796430954556 1102 60-61 0.9991116948102459 1102 62-63 0.9213646274653939 1102 64-65 0.8765418596325603 1102 66-67 0.8157862819836019 1102 68-69 0.7302801466120457 1102 70-71 0.6910185709426102 1102 72-73 0.9375392637785893 1102 74-75 0.6370676430240572 1102 76-77 0.7865292917684208 1102 78-79 0.6052728714284115 1102 80-81 0.2673713130260751 1102 82-83 0.4528088739336731 1102 84-85 0.14107010928300667 1102 86-87 0.6142933002854392 1102 88-89 0.8228437849600816 1102 90-91 0.3080197403032372 1102 92-93 0.7843546753508193 1102 94-95 0.734092128144681 1102 96-97 0.911118230432038 1102 98-99 0.9872835184574775 1102 100-101 0.909298429184183 1102 102-103 0.7808147364940687 1102 104-105 0.9525360899479978 1102 106-107 0.9402342811974052 1102 108-109 0.42852695715615496 1102 110-111 0.6327369242793459 1102 112-113 0.44661231599055995 1102 114-115 0.6336392363936163 1102 116-117 0.5434585254550655 1102 118-119 0.793183588615026 1102 120-121 1.046071311397995 1102 122-123 0.8693894413462466 1102 124-125 1.1760763594058297 1103 1 0.7823209011932022 1103 2 0.7578912785547942 1103 3 0.7814034526344749 1103 4 0.7475593943479169 1103 5 0.8774920915041733 1103 6 0.8951283309834679 1103 7 1.0193188123603036 1103 8 0.9450801938176774 1103 9 1.0978988743614906 1103 10-11 1.2031072038640005 1103 12-13 1.0850394420347556 1103 14-15 1.4502785822335298 1103 16-17 1.252904442737318 1103 18-19 1.2890367468444381 1103 20-21 1.798500860172041 1103 22-23 1.7076171217171492 1103 24-25 1.8854666657464634 1103 26-27 1.7996213245193573 1103 28-29 1.6694656319875207 1103 30-31 1.6468981880089544 1103 32-33 1.6519024336556 1103 34-35 2.023979716887439 1103 36-37 2.0214939657388875 1103 38-39 1.9889987251002452 1103 40-41 1.6589032296550315 1103 42-43 1.6276475262133872 1103 44-45 1.330319668899758 1103 46-47 1.3586227690356694 1103 48-49 1.2716046215645918 1103 50-51 1.2186592628690356 1103 52-53 1.1152976641041121 1103 54-55 1.0085112384301418 1103 56-57 0.9474045382881222 1103 58-59 0.9016263362171486 1103 60-61 0.7001202926938461 1103 62-63 0.8537334546258855 1103 64-65 0.9877301312374982 1103 66-67 0.9610629574686094 1103 68-69 0.8962082771235096 1103 70-71 0.9344048143288539 1103 72-73 0.9967048281524882 1103 74-75 1.066096523094604 1103 76-77 1.0191351118742418 1103 78-79 1.0149234446206492 1103 80-81 0.9665831736962716 1103 82-83 0.956755082046552 1103 84-85 0.9273961674840621 1103 86-87 0.8698378329133014 1103 88-89 0.9983949313445599 1103 90-91 0.9458890083808384 1103 92-93 1.1000458129169601 1103 94-95 1.0430978600670855 1103 96-97 1.1272723309611372 1103 98-99 1.102814823572114 1103 100-101 1.0574851575792437 1103 102-103 0.9677139869349851 1103 104-105 0.9839734797187205 1103 106-107 1.0052640431021658 1103 108-109 1.1626098495723838 1103 110-111 1.1679937584945144 1103 112-113 1.2832745734861497 1103 114-115 1.2278025961819736 1103 116-117 1.226687140975347 1103 118-119 1.2358092499906874 1103 120-121 1.2662763378530215 1103 122-123 1.2873237446972148 1103 124-125 1.3953279026157013 1104 1 0.8430452092180225 1104 2 0.9584847736395048 1104 3 0.8989695127334798 1104 4 0.9859927764855456 1104 5 0.9415276837791495 1104 6 0.8969837656902939 1104 7 0.902294828535446 1104 8 0.9715460656715429 1104 9 1.3558809910734766 1104 10-11 1.1900413790714168 1104 12-13 1.2012056468036363 1104 14-15 1.3581685372638574 1104 16-17 1.3583992834261522 1104 18-19 1.5351110513832396 1104 20-21 1.847078999334002 1104 22-23 1.669538224345601 1104 24-25 1.9636971178833988 1104 26-27 2.0030156196459004 1104 28-29 1.8982501435959662 1104 30-31 1.8120076926453663 1104 32-33 1.8787683264255932 1104 34-35 2.073595165189751 1104 36-37 2.0824516192981157 1104 38-39 2.1374289262436577 1104 40-41 1.7980316372406122 1104 42-43 1.943245073797577 1104 44-45 1.7774164669894184 1104 46-47 1.6753776756102994 1104 48-49 1.715014952387989 1104 50-51 1.5361229210995297 1104 52-53 1.6312930538412616 1104 54-55 1.4400507960540772 1104 56-57 1.4632132080224842 1104 58-59 1.4898551324940783 1104 60-61 1.4707577552191182 1104 62-63 1.4384592354946015 1104 64-65 1.4540277154249068 1104 66-67 1.4947276806785368 1104 68-69 1.5628039161111715 1104 70-71 1.4979331905402482 1104 72-73 1.3669727724458696 1104 74-75 1.447649589050119 1104 76-77 1.4139749292067378 1104 78-79 1.418485504510489 1104 80-81 1.4473714937827218 1104 82-83 1.4104293482391164 1104 84-85 1.4231399536515354 1104 86-87 1.4882261582287875 1104 88-89 1.4406855957801739 1104 90-91 1.4743743758074572 1104 92-93 1.4423741699552366 1104 94-95 1.3563721924940495 1104 96-97 1.319673894441582 1104 98-99 1.4251371236488062 1104 100-101 1.3698555211712389 1104 102-103 1.358289746887575 1104 104-105 1.4902988649240498 1104 106-107 1.4501720557215627 1104 108-109 1.5628503850252145 1104 110-111 1.604194997775 1104 112-113 1.605891006589406 1104 114-115 1.5711405920684705 1104 116-117 1.529466153385581 1104 118-119 1.6250744839027007 1104 120-121 1.5228235211480055 1104 122-123 1.470537246056761 1104 124-125 1.702790384314092 1105 1 0.35065975735422583 1105 2 0.5769082152263714 1105 3 0.6228123873423783 1105 4 0.6910792765275957 1105 5 0.7237304322012719 1105 6 0.5588678989756133 1105 7 0.7877896027383144 1105 8 0.4288308074652001 1105 9 0.49441949291819753 1105 10-11 0.648365242646527 1105 12-13 0.6260878588241567 1105 14-15 0.801774348065635 1105 16-17 0.6692213303170931 1105 18-19 0.7873338749740455 1105 20-21 1.0159131085765623 1105 22-23 1.2789981354628495 1105 24-25 1.3490789018780305 1105 26-27 1.2738251168610333 1105 28-29 1.0006235848593903 1105 30-31 1.0507258144203462 1105 32-33 1.0641324287464116 1105 34-35 1.6387087915069785 1105 36-37 1.6608886024595542 1105 38-39 1.699996822792933 1105 40-41 1.3177428221930754 1105 42-43 0.8932080932236985 1105 44-45 1.0421646001712297 1105 46-47 1.0388617847450448 1105 48-49 0.7993921154281125 1105 50-51 0.8335064646363399 1105 52-53 0.8553396989592912 1105 54-55 0.7414595079441355 1105 56-57 0.7498253777579329 1105 58-59 0.724108847262805 1105 60-61 0.775112622099833 1105 62-63 0.8059993480966767 1105 64-65 0.9089469942713748 1105 66-67 0.7226041332172599 1105 68-69 0.5808640208643041 1105 70-71 0.6908419768227176 1105 72-73 0.7729735444018715 1105 74-75 0.8806507081706947 1105 76-77 0.8299168988158208 1105 78-79 0.9206042865450463 1105 80-81 0.8945854441920957 1105 82-83 0.8820619058069816 1105 84-85 0.840925561200315 1105 86-87 0.7887044259422638 1105 88-89 0.918289383970972 1105 90-91 0.8195251767657155 1105 92-93 0.7750320058477413 1105 94-95 0.8748510510341916 1105 96-97 0.9359845916386043 1105 98-99 0.7755719418833564 1105 100-101 0.8502609921398694 1105 102-103 0.7903867287121074 1105 104-105 0.9160181532582392 1105 106-107 0.8516297156598469 1105 108-109 0.7831547685709097 1105 110-111 0.852727742294217 1105 112-113 0.8155585695588066 1105 114-115 0.842329167119626 1105 116-117 0.8707608400426032 1105 118-119 0.9829343113554394 1105 120-121 0.8758829897921458 1105 122-123 0.955705556184693 1105 124-125 1.0419175565185022 1106 1 0.14639049908953794 1106 2 0.46408780922845594 1106 3 0.21358106036128532 1106 4 0.37411672206383884 1106 5 0.14828673346568166 1106 6 0.210930352965903 1106 7 -0.10656105809524874 1106 8 0.17949545254831634 1106 9 0.4874957317622943 1106 10-11 0.5671535600682347 1106 12-13 0.5819114864438575 1106 14-15 0.8224754729519006 1106 16-17 0.7083866160460062 1106 18-19 0.7724760336334882 1106 20-21 1.10470231683054 1106 22-23 1.0538100967253854 1106 24-25 1.2919569194615548 1106 26-27 1.337662600689768 1106 28-29 1.2421159372194523 1106 30-31 0.9434735090040363 1106 32-33 0.7981276962553707 1106 34-35 1.191223664195526 1106 36-37 1.1736795356484642 1106 38-39 1.2904300819493315 1106 40-41 1.060634578980796 1106 42-43 0.9467276119792629 1106 44-45 0.7377148708113985 1106 46-47 0.47719270189896434 1106 48-49 0.6319930309909481 1106 50-51 0.4976452802371618 1106 52-53 0.49453002549585534 1106 54-55 0.38185033068757335 1106 56-57 0.5418536698817782 1106 58-59 0.39733038483930727 1106 60-61 0.423604567158975 1106 62-63 0.45695876558987436 1106 64-65 0.4939237063202562 1106 66-67 0.32311966361480415 1106 68-69 0.3509004120274284 1106 70-71 0.365298979096778 1106 72-73 0.38464176300768216 1106 74-75 0.44818479136911193 1106 76-77 0.2644912516217417 1106 78-79 0.29193991890521787 1106 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-3.0675285478737173 2216 90-91 -3.0659856475508995 2216 92-93 -2.973836825398486 2216 94-95 -3.144286877064765 2216 96-97 -3.0948762033080577 2216 98-99 -3.1480229826486408 2216 100-101 -3.065678378066231 2216 102-103 -2.881791414936746 2216 104-105 -3.098960734429369 2216 106-107 -3.062294620536697 2216 108-109 -3.1627204559602333 2216 110-111 -3.291030291877078 2216 112-113 -3.1702934144026464 2216 114-115 -3.29288880899281 2216 116-117 -3.355884941325783 2216 118-119 -3.500532960303836 2216 120-121 -3.54153573206165 2216 122-123 -3.4997891785067488 2216 124-125 -3.539047097384298 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 138944.0 3 24202.0 4 15642.0 5 12916.0 6 12056.0 7 12014.0 8 12196.0 9 13057.0 10 13991.0 11 15038.0 12 16057.0 13 17628.0 14 19828.0 15 17157.0 16 13216.0 17 11950.0 18 10687.0 19 10150.0 20 10155.0 21 10861.0 22 13189.0 23 17536.0 24 23456.0 25 28879.0 26 35923.0 27 40505.0 28 46396.0 29 54719.0 30 62241.0 31 71319.0 32 87770.0 33 111202.0 34 144051.0 35 198608.0 36 303572.0 37 403648.0 38 140659.0 39 474.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.40299324694288 18.433883920423437 12.568215002737727 23.594907829895966 2 15.392754241444681 20.042112178997293 40.48504287640326 24.080090703154763 3 19.390181694409186 25.656316415279086 28.095562261657836 26.85793962865389 4 12.014562165471936 16.063506663983826 36.84397595116501 35.077955219379234 5 13.332843575209965 38.92650799111865 33.546591055313165 14.194057378358227 6 32.327510299424695 36.347274245279145 17.37224379183072 13.952971663465444 7 29.19126255496719 32.57534349076223 20.400481439134357 17.832912515136222 8 26.45613926233592 35.832559268431105 19.24273641219549 18.46856505703748 9 27.070887538221346 16.286331380330115 19.14169751596605 37.50108356548248 10-11 25.044938345502427 27.216715057128727 26.84685194343517 20.89149465393368 12-13 25.798178967101965 24.596216956310084 27.215394603512223 22.390209473075732 14-15 22.65643929628269 25.815273065769873 25.16437334160771 26.36391429633973 16-17 22.573521509332704 29.29462750995837 25.416697756277078 22.715153224431848 18-19 22.3557264571986 27.76479235541621 27.572984258413502 22.306496928971693 20-21 23.241234690022576 26.980989255868025 27.780160495832472 21.997615558276923 22-23 22.909056567016513 27.001601157742666 27.53845322094129 22.550889054299528 24-25 22.499306459237143 27.14714744859129 27.79563087570472 22.55791521646685 26-27 22.562148514867296 27.46469377544057 27.397434045880352 22.57572366381178 28-29 22.37266607797993 27.29249647962746 27.73699982823 22.597837614162607 30-31 22.550902462676163 27.140376214120998 27.722278326182266 22.586442997020573 32-33 22.439507472908225 27.223382398366212 27.520158852625592 22.81695127609997 34-35 22.64433137741001 27.027010996165796 27.77918332121575 22.54947430520845 36-37 22.828224987737688 26.845700206463093 27.486123626906362 22.839951178892857 38-39 22.565505052256224 27.03100791462353 27.652525763130665 22.75096126998958 40-41 22.785124651445294 27.025484736606614 27.534047756871782 22.65534285507631 42-43 22.602922858615955 27.176402684686245 27.326218580182417 22.894455876515384 44-45 22.613193961528562 27.15391129922776 27.26246470531332 22.970430033930352 46-47 22.84492797891801 27.371501400546165 26.866533733213465 22.917036887322357 48-49 22.89066788842956 27.398717422761752 26.655939734476487 23.0546749543322 50-51 22.824871697012185 27.442045511317374 26.663273693193833 23.069809098476608 52-53 22.99937679411212 27.472202097548603 26.564173782284893 22.964247326054384 54-55 22.855361974365394 27.27389706595325 26.688666522627237 23.18207443705412 56-57 22.795175225983062 27.6094100202458 26.515748952065927 23.07966580170521 58-59 23.044581738814646 27.234903854401054 26.518191656127787 23.202322750656514 60-61 22.996545565661055 27.18087544121428 26.842480942212195 22.980098050912474 62-63 22.89422597762457 27.29825968862774 26.808260911628178 22.999253422119516 64-65 22.795413908807028 27.29453427601921 26.746814282836333 23.163237532337426 66-67 22.830100574466826 27.618183381819584 26.418288406847427 23.13342763686616 68-69 22.679845035524103 27.298616910110564 26.878214166746524 23.143323887618813 70-71 22.528450929901943 27.279998029056813 26.983352829854677 23.208198211186563 72-73 22.814853547894316 27.42389482204634 26.530613176081086 23.23063845397826 74-75 22.633574099453803 27.579894447354157 26.5556423400423 23.230889113149736 76-77 22.903527666212394 27.406424614778157 26.575550839975932 23.11449687903352 78-79 22.91098277816 27.378701637142385 26.5722434015187 23.138072183178913 80-81 22.731694097271966 27.66847306867217 26.61460676310341 22.985226070952454 82-83 22.84085079164337 27.630061073574574 26.58403382143376 22.945054313348294 84-85 22.94672821471131 27.43841849872165 26.413459239780064 23.201394046786977 86-87 22.874430800304864 27.24447921065196 26.742057324548707 23.139032664494465 88-89 22.923822934614375 27.35502055164559 26.588143927226003 23.13301258651403 90-91 22.9202364291226 27.487326820413436 26.65435816374537 22.93807858671859 92-93 22.879053054013955 27.379592335811047 26.563643464470367 23.177711145704635 94-95 22.729162350540342 27.22830764344522 26.68520585526838 23.357324150746056 96-97 22.877413752626303 27.16799971526748 26.57115655018262 23.38342998192359 98-99 22.837786296110085 27.13007225780314 26.474970732806725 23.55717071328005 100-101 22.641499533927902 27.32266105038004 26.725276620518763 23.31056279517329 102-103 22.832648688895254 27.024461059212772 26.742375992977756 23.400514258914217 104-105 22.722946819790245 27.045378645064392 26.828600079931817 23.40307445521354 106-107 22.597103916868146 27.195415466504613 26.806983831088356 23.400496785538884 108-109 22.784471132701796 27.073345767035967 26.4549530725054 23.687230027756843 110-111 22.593540283861795 27.205684261864207 26.660899918403246 23.53987553587075 112-113 22.5044162759844 27.24776129480832 26.800681785416437 23.447140643790842 114-115 22.695620952127204 27.25661209585144 26.59376009401923 23.454006858002128 116-117 22.430872506492108 27.292699640310747 26.791785361687527 23.484642491509618 118-119 22.455759681590152 27.259417891027475 26.60256527237656 23.68225715500581 120-121 22.425641244396104 27.31616839436094 26.820728498982955 23.437461862259998 122-123 22.56338359736702 27.301276705239125 26.68032914030436 23.45501055708949 124-125 22.552561357234698 27.402957285804412 26.385305066356967 23.659176290603927 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2114.0 1 2155.5 2 2821.0 3 2924.0 4 1925.5 5 1228.0 6 977.0 7 1114.5 8 1433.5 9 1550.0 10 1357.0 11 1205.0 12 1281.0 13 1414.5 14 1544.0 15 1681.0 16 1832.0 17 2032.0 18 2300.0 19 2497.0 20 2718.5 21 3300.5 22 4105.0 23 4969.0 24 6277.5 25 7937.0 26 10306.0 27 13009.5 28 15523.5 29 18784.0 30 22967.0 31 27438.5 32 32272.5 33 36253.0 34 40886.0 35 45324.5 36 49798.0 37 55224.0 38 58048.5 39 60106.0 40 61685.5 41 63756.0 42 66108.0 43 68367.0 44 71574.0 45 74762.5 46 76115.5 47 75925.0 48 78266.5 49 80322.5 50 78421.0 51 75563.5 52 74951.0 53 75756.0 54 75744.5 55 72375.5 56 67011.5 57 62876.5 58 60351.0 59 56858.0 60 50167.0 61 40986.5 62 31919.5 63 24498.5 64 18553.0 65 14179.0 66 10566.5 67 8283.0 68 6404.0 69 4539.0 70 3423.5 71 2674.0 72 2131.5 73 1835.0 74 1532.5 75 1127.5 76 806.5 77 548.5 78 361.5 79 257.0 80 184.5 81 128.5 82 98.5 83 70.5 84 49.5 85 34.5 86 29.0 87 25.5 88 16.0 89 13.0 90 13.0 91 9.5 92 8.0 93 9.5 94 10.0 95 7.5 96 5.5 97 3.5 98 2.5 99 4.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013321824250464895 2 0.005794081095236444 3 0.008668310299960034 4 0.008440196871013718 5 0.015237977053613955 6 0.0010036990873637935 7 0.0059309491526042335 8 0.0 9 0.0024636250326202204 10-11 0.0 12-13 0.002691738461566537 14-15 0.017655979400444912 16-17 0.029449443676969486 18-19 0.005383476923133074 20-21 0.008394574185224456 22-23 0.0018477187744651653 24-25 0.0109494445894232 26-27 0.017678790743339542 28-29 3.42170143419475E-4 30-31 0.0013002465449940051 32-33 0.007983970013121084 34-35 0.00791553598443719 36-37 0.009900122816270144 38-39 0.0 40-41 0.023792230639100832 42-43 0.009215782529431194 44-45 0.01569420391150659 46-47 0.003125153976564539 48-49 0.023860664667784727 50-51 0.004904438722345809 52-53 0.0 54-55 0.01653822359860796 56-57 0.0036270035202464355 58-59 3.649814863141067E-4 60-61 0.0032620220339323286 62-63 0.025548704041987468 64-65 0.016081996740715327 66-67 0.001528359973940322 68-69 0.01925277340306913 70-71 0.0021898889178846403 72-73 0.011588162190472888 74-75 0.0 76-77 0.004699136636294124 78-79 0.0010949444589423202 80-81 0.0026461157757772737 82-83 0.001254623859204742 84-85 0.0 86-87 0.004950061408135072 88-89 0.015511713168349536 90-91 0.020667076662536293 92-93 0.004014796349455174 94-95 0.022720097523053144 96-97 0.0164926009128187 98-99 8.212083442067401E-4 100-101 0.00780147926996403 102-103 0.0 104-105 0.0010949444589423202 106-107 2.281134289463167E-5 108-109 0.0 110-111 7.071516297335817E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 6.843402868389501E-5 122-123 0.0 124-125 0.02392909869646862 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2191892.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.73109646231883 #Duplication Level Percentage of deduplicated Percentage of total 1 77.17579668436859 42.239159728884836 2 11.857371741116939 12.979339131052892 3 4.427743157446361 7.270057135817069 4 2.0597604170678627 4.50931784303229 5 1.1470172784187351 3.1388756654541097 6 0.7440151265447604 2.443245819620738 7 0.49028707557070594 1.878376445910196 8 0.3504531084713825 1.5344546308213376 9 0.25714452607045224 1.2666421675007264 >10 1.3393800905737565 13.314623193200328 >50 0.09995550343786404 3.7205314385274866 >100 0.049285952808542685 4.6464473559738035 >500 0.0013633052221506171 0.5195548922304796 >1k 4.260328819220642E-4 0.539374551973583 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3713 0.1693970323355348 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2680 0.12226879791522574 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2417 0.1102700315526495 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 9.124537157852668E-5 9.124537157852668E-5 0.0 0.0 0.0 6 9.124537157852668E-5 9.124537157852668E-5 0.0 0.0 0.0 7 1.3686805736779002E-4 1.3686805736779002E-4 0.0 0.0 0.0 8 1.3686805736779002E-4 1.3686805736779002E-4 0.0 0.0 0.0 9 1.3686805736779002E-4 1.3686805736779002E-4 0.0 4.562268578926334E-5 0.0 10-11 1.3686805736779002E-4 1.3686805736779002E-4 0.0 4.562268578926334E-5 0.0 12-13 1.3686805736779002E-4 1.3686805736779002E-4 0.0 1.3686805736779002E-4 0.0 14-15 1.3686805736779002E-4 1.3686805736779002E-4 0.0 1.3686805736779002E-4 0.0 16-17 1.3686805736779002E-4 1.3686805736779002E-4 0.0 2.509247718409484E-4 0.0 18-19 1.8249074315705336E-4 1.3686805736779002E-4 0.0 3.1935880052484335E-4 0.0 20-21 2.281134289463167E-4 1.3686805736779002E-4 0.0 4.3341551499800175E-4 0.0 22-23 2.281134289463167E-4 1.3686805736779002E-4 0.0 8.896423728906352E-4 0.0 24-25 3.877928292087384E-4 1.3686805736779002E-4 0.0 0.0013686805736779 0.0 26-27 4.1060417210337004E-4 1.3686805736779002E-4 0.0 0.0016880393742027436 0.0 28-29 5.702835723657917E-4 1.3686805736779002E-4 0.0 0.0022811342894631667 0.0 30-31 6.387176010496867E-4 1.3686805736779002E-4 0.0 0.00465351395050486 0.0 32-33 6.387176010496867E-4 1.3686805736779002E-4 0.0 0.008942046414695615 0.0 34-35 7.071516297335817E-4 1.596794002624217E-4 0.0 0.014416768709407217 0.0 36-37 7.755856584174768E-4 2.0530208605168502E-4 0.0 0.022765720208842405 0.0 38-39 7.755856584174768E-4 2.965474576302117E-4 0.0 0.03893896232113626 0.0 40-41 7.755856584174768E-4 3.649814863141067E-4 0.0 0.05960603898367256 0.0 42-43 7.755856584174768E-4 3.649814863141067E-4 0.0 0.07915535984437189 0.0 44-45 8.212083442067401E-4 3.649814863141067E-4 0.0 0.1022860615395284 0.0 46-47 9.580764015745301E-4 3.649814863141067E-4 0.0 0.13171269387360326 0.0 48-49 0.0010493217731530567 3.649814863141067E-4 0.0 0.1645154049560836 0.0 50-51 0.0010493217731530567 3.649814863141067E-4 0.0 0.20379653742063936 0.0 52-53 0.0010493217731530567 3.649814863141067E-4 0.0 0.2418686687117796 0.0 54-55 0.0010493217731530567 3.649814863141067E-4 0.0 0.2821535002636991 0.0 56-57 0.0011405671447315836 3.649814863141067E-4 0.0 0.3288255078261155 0.0 58-59 0.0011405671447315836 3.649814863141067E-4 0.0 0.38633290326348196 0.0 60-61 0.001163378487626215 3.649814863141067E-4 0.0 0.4513908531989715 0.0 62-63 0.0012318125163101102 4.1060417210337004E-4 0.0 0.519482711739447 0.0 64-65 0.0012774352020993734 4.3341551499800175E-4 0.0 0.5869586640217674 4.562268578926334E-5 66-67 0.0013230578878886368 4.562268578926334E-4 0.0 0.6626238884032607 4.562268578926334E-5 68-69 0.0013230578878886368 4.562268578926334E-4 0.0 0.752591824779688 4.562268578926334E-5 70-71 0.0013914919165725318 4.7903820078726506E-4 0.0 0.8578205495526239 4.562268578926334E-5 72-73 0.0014371146023617952 5.018495436818968E-4 0.0 0.9680221470765895 4.562268578926334E-5 74-75 0.0014599259452564269 5.018495436818968E-4 0.0 1.0733649285639986 4.562268578926334E-5 76-77 0.0014599259452564269 5.246608865765285E-4 0.0 1.1931016674179202 4.562268578926334E-5 78-79 0.0014599259452564269 5.474722294711601E-4 0.0 1.3351478996227917 4.562268578926334E-5 80-81 0.0018249074315705336 5.474722294711601E-4 0.0 1.5018988161825493 4.562268578926334E-5 82-83 0.0019161528031490602 5.474722294711601E-4 0.0 1.6741244550370182 6.843402868389501E-5 84-85 0.0019617754889383237 5.474722294711601E-4 0.0 1.8464869619488553 9.124537157852668E-5 86-87 0.002007398174727587 5.474722294711601E-4 0.0 2.032057236396684 9.124537157852668E-5 88-89 0.002007398174727587 5.474722294711601E-4 0.0 2.2468488410925356 9.124537157852668E-5 90-91 0.0020302095176222184 5.474722294711601E-4 0.0 2.49460283627113 9.124537157852668E-5 92-93 0.002144266232095377 5.474722294711601E-4 0.0 2.76306953079805 9.124537157852668E-5 94-95 0.002144266232095377 5.474722294711601E-4 0.0 3.031490602639181 9.124537157852668E-5 96-97 0.002144266232095377 5.930949152604234E-4 0.0 3.2930454602690276 9.124537157852668E-5 98-99 0.0022583229465685355 5.930949152604234E-4 0.0 3.5835251006892674 9.124537157852668E-5 100-101 0.0023951910039363257 5.930949152604234E-4 0.0 3.923254430419017 9.124537157852668E-5 102-103 0.002509247718409484 6.843402868389501E-4 0.0 4.29925379535123 9.124537157852668E-5 104-105 0.002828606518934327 6.843402868389501E-4 0.0 4.680134787662896 9.124537157852668E-5 106-107 0.002897040547618222 6.843402868389501E-4 0.0 5.056886926910632 9.124537157852668E-5 108-109 0.0029426632334074855 6.843402868389501E-4 0.0 5.452093442560126 9.124537157852668E-5 110-111 0.002965474576302117 6.843402868389501E-4 0.0 5.893561361599932 9.124537157852668E-5 112-113 0.002965474576302117 7.299629726282134E-4 0.0 6.385396725751086 9.124537157852668E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3695 0.0 55.716248 1 GTATCAA 6725 0.0 53.79371 1 ATCAACG 8260 0.0 42.427315 3 TCAACGC 8295 0.0 42.248295 4 CAACGCA 8390 0.0 41.557167 5 AACGCAG 8705 0.0 39.70438 6 TATCAAC 8970 0.0 39.533394 2 GTACATG 12205 0.0 37.09942 1 TACATGG 12280 0.0 35.806046 2 ACATGGG 12580 0.0 33.91164 3 ACGCAGA 10315 0.0 33.04581 7 CGCAGAG 10285 0.0 32.62611 8 CATGGGG 5820 0.0 30.158472 4 GCAGAGT 11015 0.0 29.221552 9 CGTATAG 155 6.8346308E-6 26.87117 1 GTATAGG 770 0.0 26.272924 1 CATGGGA 8000 0.0 26.179598 4 GAGTACT 4635 0.0 25.679096 12-13 AGTACAT 8615 0.0 24.44884 2 GAGTACA 9085 0.0 24.035952 1 >>END_MODULE