FastQCFastQC Report
Thu 26 May 2016
SRR1364590_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364590_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2191892
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85670.390849549156619No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70220.32036249961220714No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52950.2415721212541494No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47250.061.83971
GTATCAA83350.050.366311
ATCAACG113000.037.0555953
TCAACGC114400.036.4979824
CAACGCA115450.035.918665
TATCAAC117250.035.5092282
AACGCAG117800.035.1516086
GTACATG112900.033.222311
TACATGG114400.031.7600142
ACATGGG116550.030.4586643
ACGCAGA141800.028.9923257
CGCAGAG142000.028.78398
CATGGGG52300.028.2440454
GCAGAGT153400.025.636429
GAGTACT89400.025.5882212-13
AGTACTT94750.025.17945912-13
TGGTATC24600.024.4547442
AGAGTAC146150.023.63113810-11
GTACTTT98200.023.2648914-15
GTACCGT2052.2662934E-623.2176046
Produced by FastQC (version 0.11.5)