FastQCFastQC Report
Thu 26 May 2016
SRR1364589_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364589_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1655078
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47990.28995612291384454No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39510.23871986697907893No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27650.1670616128061638No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19480.11769838037844743No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19320.1167316585683575No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG18600.11238141042295288No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17650.106641499675544No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA63750.045.1722561
GGTATCA38400.040.1305541
GTACATG100600.039.8037221
TACATGG100700.038.7597542
ATCAACG76250.037.454843
TCAACGC77700.036.6793024
CAACGCA78150.036.1635635
AACGCAG79200.035.6787346
TATCAAC80500.035.6252372
ACATGGG105550.035.5694963
GTATACG750.00391143631.7325781
CATGGGA72950.030.748414
ACGCAGA91500.030.49347
CGCAGAG90250.029.928788
CATGGGG38250.029.0881964
GCAGAGT95500.026.5391249
GAGTACT38200.025.31791112-13
ATGGGAG31050.024.3359725
ATGGGGA23050.024.0059345
AGTACAT70500.023.7994332
Produced by FastQC (version 0.11.5)