##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364589_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1655078 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.775896362588348 31.0 28.0 33.0 15.0 34.0 2 27.670349675362733 31.0 27.0 33.0 12.0 34.0 3 27.896586142767895 31.0 28.0 34.0 16.0 34.0 4 31.11455532609339 35.0 32.0 37.0 16.0 37.0 5 31.323784135853415 35.0 32.0 37.0 16.0 37.0 6 31.329709536348137 35.0 32.0 37.0 16.0 37.0 7 31.07628703903985 35.0 32.0 37.0 11.0 37.0 8 30.994391200898086 35.0 32.0 37.0 10.0 37.0 9 31.97761495228624 37.0 31.0 39.0 10.0 39.0 10-11 31.935048982585712 37.0 31.0 39.0 10.0 39.0 12-13 31.8582646860148 37.0 31.0 39.0 8.0 39.0 14-15 32.52684435416337 38.0 31.0 40.0 8.0 41.0 16-17 32.667219611402004 38.0 31.0 40.0 8.0 41.0 18-19 32.47055969567597 38.0 31.0 40.0 8.0 41.0 20-21 32.19759370857446 38.0 30.0 40.0 7.5 41.0 22-23 32.09096006351362 38.0 30.0 40.0 7.0 41.0 24-25 31.914240899824662 38.0 30.0 40.0 2.0 41.0 26-27 31.824033066719515 38.0 30.0 40.0 2.0 41.0 28-29 31.869004361123764 38.0 30.0 40.0 2.0 41.0 30-31 31.80157732747339 37.0 30.0 40.0 2.0 41.0 32-33 31.769528384764946 37.0 30.0 40.0 2.0 41.0 34-35 31.281775843797092 37.0 29.5 40.0 2.0 41.0 36-37 31.11786423358899 37.0 29.5 40.0 2.0 41.0 38-39 30.908126686476407 37.0 28.5 40.0 2.0 41.0 40-41 31.088473775858297 37.0 29.5 40.0 2.0 41.0 42-43 30.940555067495307 37.0 29.0 40.0 2.0 41.0 44-45 30.757576077985448 36.5 28.5 40.0 2.0 41.0 46-47 30.678832357145705 36.5 29.0 40.0 2.0 41.0 48-49 30.48535053937035 36.0 28.0 40.0 2.0 41.0 50-51 30.313590054366017 36.0 28.0 40.0 2.0 41.0 52-53 30.237387301383983 36.0 28.0 40.0 2.0 41.0 54-55 30.260332443546467 36.0 28.0 40.0 2.0 41.0 56-57 30.131180524422412 36.0 28.0 40.0 2.0 41.0 58-59 29.94001007807487 35.5 28.0 40.0 2.0 41.0 60-61 29.738464894101668 35.0 28.0 39.5 2.0 41.0 62-63 29.465945713736755 35.0 27.0 39.0 2.0 41.0 64-65 29.196048464181146 35.0 27.0 39.0 2.0 41.0 66-67 28.952262370716063 35.0 26.0 39.0 2.0 40.0 68-69 28.919065143757575 35.0 27.0 38.5 2.0 40.0 70-71 28.6941745343724 35.0 26.5 38.0 2.0 40.0 72-73 28.417778799549026 34.0 26.0 37.0 2.0 40.0 74-75 28.121142931028025 34.0 26.0 37.0 2.0 39.0 76-77 27.819150215276863 34.0 26.0 36.0 2.0 39.0 78-79 27.50226212903561 34.0 26.0 36.0 2.0 39.0 80-81 27.206909583717504 34.0 26.0 35.5 2.0 37.0 82-83 26.959352368891373 34.0 25.0 35.0 2.0 37.0 84-85 26.70869258125599 34.0 25.0 35.0 2.0 36.5 86-87 26.5013914752054 33.0 24.5 35.0 2.0 36.0 88-89 26.291136127723284 33.0 24.0 35.0 2.0 36.0 90-91 26.151181092371477 33.0 24.0 35.0 2.0 36.0 92-93 26.022717660436548 33.0 24.0 35.0 2.0 35.0 94-95 25.89451252448525 33.0 23.0 35.0 2.0 35.0 96-97 25.781060469657625 33.0 23.0 35.0 2.0 35.0 98-99 25.675265153666473 33.0 21.0 35.0 2.0 35.0 100-101 25.578920751771214 33.0 20.0 35.0 2.0 35.0 102-103 24.452323999231456 31.0 18.5 34.0 2.0 35.0 104-105 25.29635642549777 32.5 19.5 35.0 2.0 35.0 106-107 25.43828387544273 33.0 20.0 35.0 2.0 35.0 108-109 25.380965126719104 33.0 19.0 35.0 2.0 35.0 110-111 25.296038011501572 33.0 18.5 35.0 2.0 35.0 112-113 25.200969682395634 33.0 18.0 35.0 2.0 35.0 114-115 25.07122836506799 33.0 16.0 35.0 2.0 35.0 116-117 24.96000732291771 33.0 10.0 35.0 2.0 35.0 118-119 24.807704531145966 33.0 3.5 35.0 2.0 35.0 120-121 24.630357602481574 33.0 2.0 35.0 2.0 35.0 122-123 24.38603044690341 33.0 2.0 35.0 2.0 35.0 124-125 22.985156892907767 31.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.0197138704203859 1101 2 0.9550493753718712 1101 3 0.9138321223087331 1101 4 1.0859724176151708 1101 5 1.1605212676974013 1101 6 1.157026314020733 1101 7 1.133171535233899 1101 8 1.1498986131311355 1101 9 1.423438488271266 1101 10-11 1.4677395659061077 1101 12-13 1.2271731988697994 1101 14-15 1.487150509665554 1101 16-17 1.3302281163330463 1101 18-19 1.626398140810828 1101 20-21 2.0389545472405146 1101 22-23 1.9185926999623675 1101 24-25 2.091153717595848 1101 26-27 2.1171767418081906 1101 28-29 1.9637270783790406 1101 30-31 1.9464628031555016 1101 32-33 1.9550657323481637 1101 34-35 2.2758489791993526 1101 36-37 2.1701862731248376 1101 38-39 2.025993968930944 1101 40-41 1.8503039976958 1101 42-43 1.8451534105892726 1101 44-45 1.765546019241718 1101 46-47 1.5727790022578532 1101 48-49 1.6457465585762918 1101 50-51 1.6623016477308994 1101 52-53 1.4560353275647877 1101 54-55 1.3811068218805893 1101 56-57 1.2102777506699347 1101 58-59 1.3533791434288958 1101 60-61 1.3656173908687315 1101 62-63 1.2962129191546587 1101 64-65 1.4247582492772217 1101 66-67 1.2885958089261678 1101 68-69 1.113148728396613 1101 70-71 1.0805746868193857 1101 72-73 1.0255136466994372 1101 74-75 1.0979643508078674 1101 76-77 1.185553689085161 1101 78-79 1.230974239601224 1101 80-81 1.2551794794647506 1101 82-83 1.1563029274787482 1101 84-85 1.1431365501300554 1101 86-87 1.1871978704779877 1101 88-89 1.148173865522864 1101 90-91 1.200140989126382 1101 92-93 1.1663137373163863 1101 94-95 1.1595638931966548 1101 96-97 1.070490712181961 1101 98-99 1.216594132015313 1101 100-101 1.2122523472847178 1101 102-103 1.074856145876641 1101 104-105 1.1184092169785593 1101 106-107 1.1280412075452126 1101 108-109 1.158546904765231 1101 110-111 1.0184335270137872 1101 112-113 1.1776222143519952 1101 114-115 1.2813120380568144 1101 116-117 1.3390617890521348 1101 118-119 1.1454663971401722 1101 120-121 1.2931299015701647 1101 122-123 1.402642228932642 1101 124-125 1.6514274453802145 1102 1 -0.30811993472233823 1102 2 0.005975956588617493 1102 3 0.15706231286730343 1102 4 0.22747689980436903 1102 5 0.32038863683582264 1102 6 -0.5111252416396717 1102 7 -1.2432294765351202 1102 8 -0.4301684231195537 1102 9 -0.07116191538787575 1102 10-11 0.10111483785178876 1102 12-13 0.2574753207014595 1102 14-15 0.33062184724517607 1102 16-17 0.0922502455039762 1102 18-19 0.35283781473812326 1102 20-21 0.7710631420348832 1102 22-23 0.587262754482559 1102 24-25 0.7216303106078321 1102 26-27 0.4087056335803574 1102 28-29 0.8754392549930223 1102 30-31 0.6485723481215508 1102 32-33 0.5472712118899885 1102 34-35 0.909871842295999 1102 36-37 1.0682253153517784 1102 38-39 1.011354935878071 1102 40-41 0.7443221754630969 1102 42-43 0.5680461591410797 1102 44-45 0.6033964360066797 1102 46-47 0.25067161975150043 1102 48-49 0.02456084260554192 1102 50-51 0.45928911201755085 1102 52-53 0.27285271111960796 1102 54-55 0.22999659541208572 1102 56-57 0.2616622163458011 1102 58-59 0.3525293950605679 1102 60-61 0.2266171025407715 1102 62-63 0.150253769984257 1102 64-65 0.2961651258990621 1102 66-67 0.18789677744599587 1102 68-69 0.08644267875178002 1102 70-71 0.1475095312533412 1102 72-73 0.3129456602770624 1102 74-75 -0.11685963807840238 1102 76-77 0.026492327652960057 1102 78-79 -0.07510210833198983 1102 80-81 -0.35504633375115446 1102 82-83 -0.20734596114802528 1102 84-85 -0.6628283983640841 1102 86-87 -0.21206771233766375 1102 88-89 -0.053789221945354626 1102 90-91 -0.5023351910523814 1102 92-93 -0.09856529599860053 1102 94-95 -0.14228042281078856 1102 96-97 0.027224972096508537 1102 98-99 0.12036418391434367 1102 100-101 0.07911150562919289 1102 102-103 -0.08220919972373864 1102 104-105 0.10877251020809808 1102 106-107 0.020991261279061035 1102 108-109 -0.4145470180677222 1102 110-111 -0.1364184100258754 1102 112-113 -0.39471746874936287 1102 114-115 -0.45608671951179147 1102 116-117 -0.4648620923664417 1102 118-119 -0.12105561407840426 1102 120-121 0.18774275575485078 1102 122-123 0.24216445639719097 1102 124-125 0.5197149738853639 1103 1 0.3389414979678378 1103 2 0.27740593613569686 1103 3 0.22589748203514048 1103 4 0.35324758066486694 1103 5 0.35591265053681553 1103 6 0.39915543158178934 1103 7 0.4822997120013852 1103 8 0.44493322543518943 1103 9 0.7175470863617122 1103 10-11 0.7134297142717898 1103 12-13 0.6715792116849215 1103 14-15 0.9102625658080541 1103 16-17 0.6610250415908112 1103 18-19 0.7175146339392242 1103 20-21 1.1211592461230069 1103 22-23 1.1370877957580419 1103 24-25 1.1623328561340607 1103 26-27 1.1340376855258505 1103 28-29 0.9828947144444875 1103 30-31 1.0132676488780668 1103 32-33 0.9170267625171284 1103 34-35 1.1906473283609103 1103 36-37 1.1896602233136342 1103 38-39 1.2341069626886458 1103 40-41 0.9032943545616909 1103 42-43 0.9471903399153341 1103 44-45 0.6293371471769333 1103 46-47 0.7400052980738678 1103 48-49 0.6643368461540007 1103 50-51 0.6415492830335481 1103 52-53 0.5815772373757397 1103 54-55 0.6236759034626509 1103 56-57 0.6696129814821958 1103 58-59 0.5628457992891498 1103 60-61 0.4510300051306544 1103 62-63 0.44075425050469974 1103 64-65 0.5505341655974441 1103 66-67 0.5480554724017637 1103 68-69 0.5762310033371776 1103 70-71 0.5325296145435559 1103 72-73 0.6074800482911549 1103 74-75 0.5910603244656443 1103 76-77 0.6269555986418425 1103 78-79 0.6261090495990622 1103 80-81 0.7391824925468669 1103 82-83 0.6746702533365898 1103 84-85 0.5663317997581885 1103 86-87 0.4985653425402319 1103 88-89 0.5819775161833256 1103 90-91 0.6187276215939335 1103 92-93 0.5712789660944999 1103 94-95 0.598326018627322 1103 96-97 0.7226403142517093 1103 98-99 0.6914109792126375 1103 100-101 0.6434766025957543 1103 102-103 0.6343022218775012 1103 104-105 0.6366389994518329 1103 106-107 0.6455261421346314 1103 108-109 0.7404011103667685 1103 110-111 0.7778949385855469 1103 112-113 0.8234646854607348 1103 114-115 0.8786142489217106 1103 116-117 0.8454210450624053 1103 118-119 0.9346317765723917 1103 120-121 0.9474985528027311 1103 122-123 0.896361001578434 1103 124-125 1.039527201282418 1104 1 0.5519190516324386 1104 2 0.5620296711218629 1104 3 0.481732011806006 1104 4 0.6268921044827316 1104 5 0.5666957626751135 1104 6 0.5130679574073298 1104 7 0.6575935687323664 1104 8 0.7459849232092317 1104 9 0.9584243960697805 1104 10-11 1.0268566615468373 1104 12-13 0.9210988857870177 1104 14-15 0.9167816912997608 1104 16-17 0.8843771677478571 1104 18-19 1.2163964025657137 1104 20-21 1.4991658061024609 1104 22-23 1.3657661116408875 1104 24-25 1.7191070270903985 1104 26-27 1.7569404180724568 1104 28-29 1.6266738228942579 1104 30-31 1.6322413972876753 1104 32-33 1.5158980258384425 1104 34-35 1.7883968585403096 1104 36-37 1.7722214167163024 1104 38-39 1.7924032356398207 1104 40-41 1.4890806766543427 1104 42-43 1.639726904213635 1104 44-45 1.364280126714931 1104 46-47 1.3309077037887533 1104 48-49 1.3698824800324445 1104 50-51 1.0893519233669124 1104 52-53 1.1198854163394465 1104 54-55 0.968398154866275 1104 56-57 1.0112723527980414 1104 58-59 1.0339783879130735 1104 60-61 1.02068251298045 1104 62-63 0.9996364860336406 1104 64-65 0.9921690245345438 1104 66-67 1.0655012825358803 1104 68-69 1.0505968914440018 1104 70-71 1.1028918141210085 1104 72-73 1.0207645903182119 1104 74-75 1.1258737385731017 1104 76-77 1.1311273720540846 1104 78-79 1.1987228862532397 1104 80-81 1.1557832092421307 1104 82-83 1.1147246474722898 1104 84-85 1.1416875210641138 1104 86-87 1.1286513693170903 1104 88-89 1.092203847147129 1104 90-91 1.2619814647847427 1104 92-93 1.2275663912441992 1104 94-95 1.2283606861344118 1104 96-97 1.0747991544660174 1104 98-99 1.065880753115124 1104 100-101 1.0544418066904875 1104 102-103 1.1079467456953616 1104 104-105 1.191910911767554 1104 106-107 1.1767525781514898 1104 108-109 1.2403695944512307 1104 110-111 1.316462248410339 1104 112-113 1.3162311992168476 1104 114-115 1.3469022343543529 1104 116-117 1.3480683819675399 1104 118-119 1.2973437794671874 1104 120-121 1.3018319911891147 1104 122-123 1.3064811122343158 1104 124-125 1.535634402085492 1105 1 0.12889388160182236 1105 2 0.38814793176825546 1105 3 0.4613607248285483 1105 4 0.582951182864857 1105 5 0.46626373520134123 1105 6 0.4065595887846243 1105 7 0.5779407183898613 1105 8 0.4594335351605636 1105 9 0.3185525988477096 1105 10-11 0.6445111251178091 1105 12-13 0.54488018028065 1105 14-15 0.721766047872137 1105 16-17 0.7768049238326 1105 18-19 0.7240597725022226 1105 20-21 1.0558090687348312 1105 22-23 1.250337628381189 1105 24-25 1.3095174837440489 1105 26-27 1.0725077673058614 1105 28-29 0.9426177448726598 1105 30-31 0.9897428333054457 1105 32-33 0.953190719868882 1105 34-35 1.5594475805211587 1105 36-37 1.6607656613403954 1105 38-39 1.6245484934202885 1105 40-41 1.2158921214104375 1105 42-43 0.8375067290805767 1105 44-45 1.0701322810471225 1105 46-47 1.080863706648568 1105 48-49 0.8578912733497646 1105 50-51 0.9369538500750139 1105 52-53 0.8878647407389195 1105 54-55 0.5979999043636717 1105 56-57 0.6028891407623824 1105 58-59 0.6121364812481147 1105 60-61 0.716919229816078 1105 62-63 0.7807885236511929 1105 64-65 0.7997164436745194 1105 66-67 0.671384754276886 1105 68-69 0.5187217272185691 1105 70-71 0.6917545033239847 1105 72-73 0.7339757246500405 1105 74-75 0.7201253136096142 1105 76-77 0.7293584731436269 1105 78-79 0.8331065749245852 1105 80-81 0.6708981364940847 1105 82-83 0.5611727186254463 1105 84-85 0.6572899305186084 1105 86-87 0.5551714094033713 1105 88-89 0.724325578679025 1105 90-91 0.5962515705612006 1105 92-93 0.4791207277123313 1105 94-95 0.7079872604070836 1105 96-97 0.8196851743747224 1105 98-99 0.689492246145857 1105 100-101 0.6485112824523398 1105 102-103 0.6334938073585796 1105 104-105 0.7190796041358816 1105 106-107 0.6325200227120362 1105 108-109 0.7482361456254054 1105 110-111 0.7737216237731666 1105 112-113 0.7451370147832783 1105 114-115 0.807107966815078 1105 116-117 0.8949452724696592 1105 118-119 1.004911593114855 1105 120-121 0.8712065443431598 1105 122-123 0.9259784907380464 1105 124-125 1.054131456721386 1106 1 0.3675128122530964 1106 2 0.6548234458057358 1106 3 0.3887421334137464 1106 4 0.471424053370761 1106 5 0.3983536203006963 1106 6 0.43100675898709895 1106 7 0.04772197369043241 1106 8 0.3040382618553359 1106 9 0.5579712480583581 1106 10-11 0.6561858852964733 1106 12-13 0.6493335227021717 1106 14-15 0.9815236508523952 1106 16-17 0.7694486752853464 1106 18-19 0.811493809855925 1106 20-21 1.0981407540490409 1106 22-23 1.146970235287796 1106 24-25 1.2569804347243796 1106 26-27 1.4842550863954465 1106 28-29 1.2553534042792442 1106 30-31 1.1196024742173627 1106 32-33 1.151522100498486 1106 34-35 1.4090690020476018 1106 36-37 1.4291211811574591 1106 38-39 1.5691083026940014 1106 40-41 1.2252020421924392 1106 42-43 1.2776608617974183 1106 44-45 1.1447928089995756 1106 46-47 0.9463737235475698 1106 48-49 1.124341886174161 1106 50-51 1.0591101532770253 1106 52-53 1.0835304451885683 1106 54-55 0.9822121376075863 1106 56-57 1.1078323343596104 1106 58-59 1.0591413844714879 1106 60-61 0.9563300263307397 1106 62-63 0.9054481092801332 1106 64-65 0.9883235167548499 1106 66-67 0.7480054722017613 1106 68-69 0.8801890191692436 1106 70-71 1.0924842543621125 1106 72-73 1.0984904123326125 1106 74-75 1.1459169438921215 1106 76-77 1.0242367877666005 1106 78-79 1.0106945879412166 1106 80-81 0.9647281947296769 1106 82-83 1.0236188491309726 1106 84-85 0.956893762006036 1106 86-87 1.0950469284665765 1106 88-89 0.9795239063688861 1106 90-91 0.9535341608200909 1106 92-93 1.0136051353991782 1106 94-95 1.1166600919636132 1106 96-97 1.0251595243285472 1106 98-99 0.8895729718606091 1106 100-101 0.9480774209990273 1106 102-103 0.9864800305887407 1106 104-105 0.9683843264331422 1106 106-107 0.9715050460502503 1106 108-109 0.9201994295600429 1106 110-111 0.788554581224119 1106 112-113 0.9228670830703223 1106 114-115 0.9857846776034229 1106 116-117 0.8921284318919511 1106 118-119 0.8493374353950252 1106 120-121 0.8491441593851583 1106 122-123 0.9611600607746702 1106 124-125 1.0922650122336641 1107 1 0.4522389298441567 1107 2 0.4367864280029252 1107 3 0.3486146901670466 1107 4 0.5434044278042514 1107 5 0.40342234997396176 1107 6 0.4092291889039501 1107 7 0.43834829120946495 1107 8 0.2620882194189065 1107 9 0.28417647014232017 1107 10-11 0.5514669704711537 1107 12-13 0.5843245428864705 1107 14-15 0.7322341162188337 1107 16-17 0.4355572983513767 1107 18-19 0.7277322639564261 1107 20-21 1.2304546937648837 1107 22-23 1.2632791409481072 1107 24-25 1.145087990772378 1107 26-27 1.4640765460666607 1107 28-29 1.316799221592202 1107 30-31 1.123153421964222 1107 32-33 0.7839751308218048 1107 34-35 1.4130791458113379 1107 36-37 1.3958618193351136 1107 38-39 1.2581149435809742 1107 40-41 0.9993986461750843 1107 42-43 0.8414919451201825 1107 44-45 0.5892525503462096 1107 46-47 0.631794900151661 1107 48-49 0.5384981578208752 1107 50-51 0.7782796759637662 1107 52-53 0.6907717465092595 1107 54-55 0.6117285830032948 1107 56-57 0.5474117684933084 1107 58-59 0.22486764627547728 1107 60-61 0.5098386952612053 1107 62-63 0.4748625969262861 1107 64-65 0.44472180290638974 1107 66-67 0.4607861259197392 1107 68-69 0.3300941775000581 1107 70-71 0.536922087953009 1107 72-73 0.5899144716190996 1107 74-75 0.3785178121642545 1107 76-77 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78-79 -2.554600141999259 2216 80-81 -2.4832687233208404 2216 82-83 -2.192255783683642 2216 84-85 -2.339783511209525 2216 86-87 -2.352425835916165 2216 88-89 -2.4293218813247996 2216 90-91 -2.4202174088627153 2216 92-93 -2.4098950998217283 2216 94-95 -2.4341583656540244 2216 96-97 -2.4731854095317765 2216 98-99 -2.5671041531843066 2216 100-101 -2.496109659261311 2216 102-103 -2.424356800189269 2216 104-105 -2.7316019677819092 2216 106-107 -2.6231789615212726 2216 108-109 -2.6632721346404438 2216 110-111 -2.7006322953015776 2216 112-113 -2.672350338834484 2216 114-115 -2.8192801655764654 2216 116-117 -2.9169444273200007 2216 118-119 -2.8919162364588473 2216 120-121 -2.983967535353674 2216 122-123 -3.0198952105813497 2216 124-125 -3.16180986580337 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 120848.0 3 20315.0 4 13918.0 5 12094.0 6 11542.0 7 10437.0 8 10719.0 9 11136.0 10 12159.0 11 13072.0 12 13480.0 13 15268.0 14 17351.0 15 15437.0 16 11592.0 17 10221.0 18 9450.0 19 8780.0 20 8626.0 21 8904.0 22 10868.0 23 14808.0 24 20917.0 25 24176.0 26 29991.0 27 32568.0 28 36488.0 29 42609.0 30 47630.0 31 53970.0 32 65853.0 33 82551.0 34 106601.0 35 145376.0 36 213356.0 37 275869.0 38 95708.0 39 390.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.34173838901622 18.95512476153817 12.596102308509938 23.10703454093567 2 14.88969466409909 21.66420845475853 40.02164354376473 23.424453337377653 3 18.861703040461745 27.29660061415901 28.214153211041328 25.627543134337916 4 11.617964913468006 17.314677235447633 37.09555106931563 33.971806781768734 5 12.862520160571512 40.21551341248303 32.7781131578359 14.143853269109563 6 30.461480643648827 37.55449940304423 17.988972027667813 13.99504792563913 7 28.06842097948494 34.33816878165917 20.28169150254603 17.311718736309853 8 25.951707411976958 36.27206693581813 19.58276286676519 18.19346278543972 9 27.129055062446 17.404699612695843 19.178081364084036 36.28816396077412 10-11 24.424498422430847 28.498143289923494 26.771759397442295 20.30559889020336 12-13 25.134085333044915 26.089185590777575 27.12803159135945 21.648697484818065 14-15 22.132554322470853 27.312527345553523 25.44215191678733 25.112766415188293 16-17 21.694078952338675 30.763084661036437 25.732171991631713 21.810664394993175 18-19 21.394941186351684 28.94186189140246 28.31816591569772 21.345031006548144 20-21 22.820722913989076 28.147559338973416 28.007524135301498 21.02419361173601 22-23 22.17137415753521 28.230172149969835 27.767131841868427 21.831321850626527 24-25 21.785104730241112 28.172188619878792 28.287996606430198 21.754710043449897 26-27 21.78961849766175 28.579392606966387 28.023874149763667 21.607114745608204 28-29 21.575292080761805 28.419798745003593 28.386929935359014 21.617979238875584 30-31 21.716343668723713 28.344243935877138 28.164822453092565 21.774589942306584 32-33 21.658335410482426 28.39987008376576 27.956372315756877 21.98542218999494 34-35 21.922889833555402 28.20950447306643 28.069103664560807 21.798502028817364 36-37 22.107742276606995 28.046801117909208 27.927063977641815 21.918392627841982 38-39 21.810452437891144 28.151996461798173 28.09722562924527 21.94032547106541 40-41 21.834699266137214 28.300403283073532 28.02566555607194 21.83923189471731 42-43 22.061871056997376 28.213989949299346 27.602234442427214 22.121904551276067 44-45 21.80895728214656 28.434232659214096 27.551847636221567 22.204962422417772 46-47 21.91857343849745 28.667393151844966 27.050958594876555 22.363074814781022 48-49 22.082569708324204 28.349769212754865 27.212377146396925 22.355283932524003 50-51 22.229311793925486 28.673400148518468 26.790121890281227 22.30716616727482 52-53 22.364142354620146 28.69529411906871 26.629681501415643 22.310882024895502 54-55 22.029805955812883 28.52139241909991 26.86228304448232 22.586518580604885 56-57 22.017873829411002 28.967978346690415 26.7338856228393 22.28026220105928 58-59 22.153396812890787 28.4091310748435 26.905934993219333 22.531537119046373 60-61 22.25590477793129 28.49837265320217 27.13776390193442 22.107958666932127 62-63 22.008683507078015 28.61393265326227 26.94268389749739 22.434699942162332 64-65 21.954356746173232 28.42010164431741 26.938372844892704 22.687168764616658 66-67 22.05839880026634 28.846476869961585 26.603562222890893 22.491562106881187 68-69 22.05197811950151 28.578356823272642 26.959921509165774 22.409743548060074 70-71 21.96108085637584 28.522689440194416 27.09956550400267 22.41666419942708 72-73 22.049255094570615 28.550261666368055 26.86729416641564 22.53318907264569 74-75 21.918000239263648 28.851903052303275 26.642218674890245 22.58787803354283 76-77 22.047081614254395 28.581122398420263 26.827782732592286 22.544013254733056 78-79 22.074221500798007 28.631780307596195 26.89371590568194 22.400282285923858 80-81 21.869800826037057 29.16721676388156 26.81460329103652 22.148379119044865 82-83 22.12914967061239 28.972665933955717 26.516352162876935 22.381832232554963 84-85 22.284901376249337 28.53690279249679 26.507814133231182 22.67038169802269 86-87 22.16091873457678 28.297880568372154 26.813988393761345 22.727212303289722 88-89 22.18088414009571 28.30244207246509 26.91029579104934 22.60637799638986 90-91 22.061843894824342 28.69232672900834 26.808630091826398 22.437199284340924 92-93 22.06176472365392 28.355760457767115 26.899256188859148 22.683218629719818 94-95 22.017603446233583 28.46675119479584 26.79249593273849 22.723149426232084 96-97 22.126992385944018 28.447513957390413 26.79072631609799 22.634767340567578 98-99 22.14677003864214 28.44811829914169 26.620267501118537 22.784844161097627 100-101 21.882565497780107 28.46979239209087 26.860532743980457 22.787109366148567 102-103 22.085605633087987 28.10924923175826 26.987972772280216 22.817172362873535 104-105 21.88540378938108 28.33709757916094 26.90179004088104 22.875708590576938 106-107 21.913445140786457 28.465525028284173 26.860796548802096 22.76023328212727 108-109 22.01724027508069 28.534063047179647 26.468510849639713 22.980185828099945 110-111 21.794088263078727 28.724456795766447 26.776797385719643 22.704657555435183 112-113 21.88649115026603 28.520710202177785 26.653577656158806 22.939220991397384 114-115 21.44675356690138 28.739098701088405 26.69022245477252 23.12392527723769 116-117 21.496962680912922 28.735382864130877 26.864051120249314 22.903603334706883 118-119 21.648647374927346 28.79909587342711 26.54291821896007 23.00933853268547 120-121 21.636079991396176 28.76589502126184 26.72348372705093 22.874541260291057 122-123 21.72474650741536 28.777918623774827 26.5608327825033 22.936502086306508 124-125 21.778703507010306 28.87358608642259 26.321901356251075 23.025809050316028 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4040.0 1 4259.5 2 5266.0 3 5032.5 4 3202.5 5 1993.0 6 1499.5 7 1584.0 8 1983.5 9 2124.5 10 1740.0 11 1383.5 12 1393.5 13 1534.5 14 1644.5 15 1728.5 16 1906.5 17 2104.5 18 2304.5 19 2515.0 20 2845.0 21 3414.5 22 4120.0 23 4977.0 24 6045.0 25 7567.5 26 9635.5 27 12052.0 28 14493.5 29 16875.5 30 19926.5 31 23563.5 32 27403.0 33 31110.0 34 34614.0 35 37286.0 36 40392.0 37 44168.0 38 45751.0 39 46382.0 40 47232.5 41 49832.0 42 53162.0 43 55122.0 44 56403.0 45 57271.0 46 57011.0 47 56208.0 48 58946.5 49 60177.5 50 57644.5 51 55843.5 52 55189.5 53 54359.0 54 52556.0 55 48649.5 56 42894.5 57 38542.0 58 36018.0 59 33349.5 60 29722.0 61 25501.0 62 21031.0 63 16206.5 64 12243.0 65 9708.5 66 7452.5 67 6151.5 68 4740.5 69 3123.0 70 2389.5 71 2036.0 72 1826.0 73 1628.0 74 1314.0 75 1076.5 76 782.5 77 511.0 78 355.0 79 257.0 80 187.5 81 141.5 82 106.0 83 76.0 84 57.0 85 40.0 86 31.5 87 25.0 88 19.5 89 16.0 90 13.0 91 11.0 92 9.0 93 8.5 94 7.0 95 5.5 96 3.5 97 4.0 98 4.0 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012144442739254585 2 0.004894029163580206 3 0.007975454933241817 4 0.008519235951417396 5 0.01444040703821814 6 8.458815838286775E-4 7 0.008881756630201115 8 0.0 9 0.0029001654302697514 10-11 0.0 12-13 0.004138777749447458 14-15 0.019938637333104543 16-17 0.030059006282483366 18-19 0.006344111878715081 20-21 0.008277555498894916 22-23 0.002326174355528863 24-25 0.011087090759468739 26-27 0.019183385918971795 28-29 8.156715272633676E-4 30-31 0.0022053341292676237 32-33 0.00999952872311758 34-35 0.01045267957159723 36-37 0.012265282965515824 38-39 0.0 40-41 0.02471182627042351 42-43 0.010180789062509441 44-45 0.018216664108881876 46-47 0.004682558767623036 48-49 0.025104557005772538 50-51 0.003927307353490289 52-53 0.0 54-55 0.016585321054355142 56-57 0.005347180012059854 58-59 2.718905090877892E-4 60-61 0.005045079446406755 62-63 0.027007790569387062 64-65 0.017400992581618507 66-67 0.002658484977747272 68-69 0.021902291009849688 70-71 0.00344394644844533 72-73 0.012295493022081133 74-75 0.0 76-77 0.007371253801935619 78-79 0.0011177720929164668 80-81 0.004078357636316838 82-83 0.0022355441858329336 84-85 0.0 86-87 0.006404531991845702 88-89 0.017823933373532847 90-91 0.02292943293307022 92-93 0.005316969955494544 94-95 0.02428888547850917 96-97 0.018700025013926837 98-99 0.0011781922060470867 100-101 0.009395327591811383 102-103 0.0 104-105 0.0019334436201798343 106-107 3.021005656530991E-5 108-109 0.0 110-111 8.760916403939875E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 0.0 122-123 0.0 124-125 0.024560775987596963 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1655078.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.959043831913554 #Duplication Level Percentage of deduplicated Percentage of total 1 76.68766197532956 41.37992913893778 2 11.4194567266133 12.323659320959344 3 4.6990094712028245 7.606621740696304 4 2.2930658249674845 4.949265574355341 5 1.350695314619451 3.6441113842555612 6 0.8388448862165488 2.7157960790121174 7 0.5647653667139725 2.1331939424086177 8 0.41128084183981023 1.77538567776485 9 0.29171189143360665 1.416644526254075 >10 1.3130123028367973 12.091230600679859 >50 0.07950887458494676 3.00361504521596 >100 0.0478861476993408 4.780776952489578 >500 0.0018372598177109956 0.6539929655805926 >1k 0.0012631161246763095 1.525777051390061 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4799 0.28995612291384454 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3951 0.23871986697907893 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2765 0.1670616128061638 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1948 0.11769838037844743 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1932 0.1167316585683575 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 1860 0.11238141042295288 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1765 0.106641499675544 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 6.042011313061982E-5 0.0 0.0 0.0 0.0 8 6.042011313061982E-5 0.0 0.0 0.0 0.0 9 6.042011313061982E-5 0.0 0.0 0.0 0.0 10-11 6.042011313061982E-5 0.0 0.0 6.042011313061982E-5 0.0 12-13 1.2084022626123965E-4 0.0 0.0 6.042011313061982E-5 0.0 14-15 1.2084022626123965E-4 0.0 0.0 1.2084022626123965E-4 0.0 16-17 1.2084022626123965E-4 0.0 0.0 1.5105028282654957E-4 0.0 18-19 1.5105028282654957E-4 0.0 0.0 3.0210056565309915E-4 0.0 20-21 1.812603393918595E-4 0.0 0.0 4.229407919143388E-4 0.0 22-23 1.812603393918595E-4 0.0 0.0 9.063016969592973E-4 0.0 24-25 2.416804525224793E-4 0.0 0.0 0.0015407128848308055 0.0 26-27 2.416804525224793E-4 0.0 0.0 0.0025980648646166523 0.0 28-29 3.927307353490289E-4 0.0 0.0 0.0035949967312718795 0.0 30-31 4.229407919143388E-4 0.0 0.0 0.0074618839716315485 0.0 32-33 4.229407919143388E-4 0.0 0.0 0.014772717660436547 0.0 34-35 4.5315084847964867E-4 0.0 0.0 0.02380552457346421 0.0 36-37 4.833609050449586E-4 0.0 0.0 0.03951475398742536 0.0 38-39 5.437810181755785E-4 0.0 0.0 0.06727779597094517 0.0 40-41 5.437810181755785E-4 0.0 0.0 0.10017654757056768 0.0 42-43 6.042011313061983E-4 0.0 0.0 0.13253151815201458 0.0 44-45 7.854614706980578E-4 0.0 0.0 0.16805854467281905 0.0 46-47 9.969318666552271E-4 0.0 0.0 0.21177249652282248 0.0 48-49 0.001027141923220537 0.0 0.0 0.2615284596858879 0.0 50-51 0.001027141923220537 0.0 0.0 0.31774937495392963 0.0 52-53 0.001027141923220537 0.0 0.0 0.371885796318965 0.0 54-55 0.001027141923220537 0.0 0.0 0.43185275860110517 0.0 56-57 0.001087562036351157 0.0 0.0 0.5028463915295835 0.0 58-59 0.001087562036351157 0.0 0.0 0.5893679935326311 0.0 60-61 0.001087562036351157 0.0 0.0 0.6860401745416229 0.0 62-63 0.001087562036351157 0.0 0.0 0.7813529030051756 0.0 64-65 0.001087562036351157 0.0 0.0 0.8721945430970625 0.0 66-67 0.0011479821494817766 0.0 0.0 0.9806788562230904 0.0 68-69 0.0011479821494817766 0.0 0.0 1.1075308837408266 0.0 70-71 0.0011479821494817766 0.0 0.0 1.2574936045310252 0.0 72-73 0.0012084022626123966 0.0 0.0 1.4095106091676646 0.0 74-75 0.0012688223757430163 0.0 0.0 1.5616484540305655 0.0 76-77 0.0012688223757430163 0.0 0.0 1.7291632176852088 0.0 78-79 0.0012688223757430163 0.0 0.0 1.9267369876223355 0.0 80-81 0.001419872658569566 0.0 0.0 2.146968299983445 0.0 82-83 0.001450082715134876 0.0 0.0 2.3813379188171195 0.0 84-85 0.001450082715134876 0.0 0.0 2.6149220761800955 0.0 86-87 0.001450082715134876 0.0 0.0 2.853037742027868 0.0 88-89 0.001450082715134876 0.0 0.0 3.1257439226429207 0.0 90-91 0.0015709229413961156 0.0 0.0 3.443734978049373 0.0 92-93 0.0015709229413961156 0.0 0.0 3.786981640744424 0.0 94-95 0.0015709229413961156 3.021005656530991E-5 0.0 4.1288990609506016 0.0 96-97 0.0015709229413961156 6.042011313061982E-5 0.0 4.472085303532522 0.0 98-99 0.0016313430545267353 6.042011313061982E-5 0.0 4.83693215667177 0.0 100-101 0.001721973224222665 6.042011313061982E-5 0.0 5.262108492771942 0.0 102-103 0.0018126033939185949 6.042011313061982E-5 0.0 5.7239598375424 0.0 104-105 0.0021449140161370036 6.042011313061982E-5 0.0 6.191128152268353 0.0 106-107 0.002326174355528863 6.042011313061982E-5 0.0 6.652828446755984 0.0 108-109 0.0023865944686594833 6.042011313061982E-5 0.0 7.1304796511100985 0.0 110-111 0.002416804525224793 6.042011313061982E-5 0.0 7.672810586570542 0.0 112-113 0.002416804525224793 6.042011313061982E-5 0.0 8.26921752328289 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 6375 0.0 45.172256 1 GGTATCA 3840 0.0 40.130554 1 GTACATG 10060 0.0 39.803722 1 TACATGG 10070 0.0 38.759754 2 ATCAACG 7625 0.0 37.45484 3 TCAACGC 7770 0.0 36.679302 4 CAACGCA 7815 0.0 36.163563 5 AACGCAG 7920 0.0 35.678734 6 TATCAAC 8050 0.0 35.625237 2 ACATGGG 10555 0.0 35.569496 3 GTATACG 75 0.003911436 31.732578 1 CATGGGA 7295 0.0 30.74841 4 ACGCAGA 9150 0.0 30.4934 7 CGCAGAG 9025 0.0 29.92878 8 CATGGGG 3825 0.0 29.088196 4 GCAGAGT 9550 0.0 26.539124 9 GAGTACT 3820 0.0 25.317911 12-13 ATGGGAG 3105 0.0 24.335972 5 ATGGGGA 2305 0.0 24.005934 5 AGTACAT 7050 0.0 23.799433 2 >>END_MODULE