FastQCFastQC Report
Thu 26 May 2016
SRR1364589_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364589_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1655078
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115920.700389951410145No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91660.5538107569552613No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72850.4401605241565654No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25840.15612557232952162No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21450.12960114266517953No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21180.1279697996106528No Hit
TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC19280.11648997811583503No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG17860.107910322051287No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG17560.1060977186573684No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17430.10531225718667037No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54800.059.2809681
GTATCAA99650.051.1432271
ATCAACG129750.039.244343
TATCAAC131600.038.4211272
TCAACGC132350.038.20344
CAACGCA133750.037.633525
AACGCAG136800.036.8379556
GTACATG99800.036.2541281
TACATGG98800.035.8656162
ACATGGG103000.033.535833
ACGCAGA156550.031.9625287
CGCAGAG156850.031.939338
GCAGAGT164750.029.613299
CATGGGA70550.029.545364
GAGTACT107300.028.72289812-13
GACGGGT1055.908251E-428.332118
AGTACTT110650.027.36935412-13
CATGGGG36650.027.299434
ATGGGAG26900.026.9839655
CGCGATT450.00953490826.44250536-37
Produced by FastQC (version 0.11.5)