##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364589_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1655078 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.892339213015944 31.0 30.0 34.0 26.0 34.0 2 29.785037321503882 31.0 30.0 34.0 26.0 34.0 3 30.050756520236508 31.0 30.0 34.0 26.0 34.0 4 33.580026439841504 35.0 35.0 37.0 28.0 37.0 5 32.99938794425399 35.0 33.0 37.0 26.0 37.0 6 33.0053767858675 35.0 33.0 37.0 26.0 37.0 7 32.781671317001376 35.0 33.0 37.0 25.0 37.0 8 32.70122435317248 35.0 33.0 37.0 25.0 37.0 9 33.8913483231606 38.0 33.0 39.0 23.0 39.0 10-11 33.82397536551147 38.0 33.0 39.0 23.0 39.0 12-13 33.70109596043208 38.0 33.0 39.0 22.5 39.0 14-15 34.607568646311535 39.0 33.0 41.0 21.5 41.0 16-17 34.64047827353152 39.0 33.0 41.0 22.0 41.0 18-19 34.69032456476371 39.0 33.5 41.0 22.5 41.0 20-21 34.72237985158404 39.0 34.0 41.0 23.0 41.0 22-23 34.66833828979661 39.0 34.0 41.0 23.0 41.0 24-25 34.61815485433315 39.0 34.0 41.0 23.0 41.0 26-27 34.47525524476792 39.0 33.0 41.0 22.0 41.0 28-29 34.30031726601405 38.5 33.0 41.0 21.5 41.0 30-31 34.08002583564037 38.0 33.0 40.0 20.0 41.0 32-33 33.80807128123267 38.0 33.0 40.0 18.5 41.0 34-35 33.5752786273517 38.0 33.0 40.0 16.5 41.0 36-37 33.32985242991569 38.0 32.0 40.0 15.5 41.0 38-39 33.14244404191223 38.0 32.0 40.0 14.5 41.0 40-41 33.12554755727525 38.0 32.0 40.0 11.0 41.0 42-43 32.95130743082804 38.0 32.0 40.0 9.0 41.0 44-45 32.72147173728368 38.0 31.5 40.0 7.0 41.0 46-47 32.378859183675935 38.0 31.0 40.0 2.0 41.0 48-49 32.02114794589741 38.0 30.0 40.0 2.0 41.0 50-51 31.83163452115248 37.0 30.0 40.0 2.0 41.0 52-53 31.658301602703922 37.0 30.0 40.0 2.0 41.0 54-55 31.479712738614133 37.0 30.0 40.0 2.0 41.0 56-57 31.2696915190704 36.5 30.0 40.0 2.0 41.0 58-59 31.065139830267817 36.0 29.5 40.0 2.0 41.0 60-61 30.829814667344984 36.0 29.0 40.0 2.0 41.0 62-63 30.658364137521012 35.0 29.0 39.5 2.0 41.0 64-65 30.53217763754941 35.0 29.0 39.0 2.0 41.0 66-67 30.23463879043767 35.0 29.0 39.0 2.0 41.0 68-69 29.96574240005607 35.0 29.0 39.0 2.0 41.0 70-71 29.666767668955785 35.0 28.5 38.0 2.0 40.0 72-73 29.38599902844458 35.0 28.0 37.0 2.0 40.0 74-75 29.044559229232704 35.0 28.0 37.0 2.0 39.0 76-77 28.738577577612656 34.5 28.0 36.0 2.0 39.0 78-79 28.44902868626131 34.0 28.0 36.0 2.0 39.0 80-81 28.172868589879148 34.0 27.0 35.5 2.0 37.5 82-83 27.8787610009921 34.0 27.0 35.0 2.0 37.0 84-85 27.645497976530415 34.0 26.0 35.0 2.0 36.5 86-87 27.41803347032587 34.0 26.0 35.0 2.0 36.0 88-89 27.231633494010552 34.0 26.0 35.0 2.0 36.0 90-91 27.078315342237648 34.0 26.0 35.0 2.0 36.0 92-93 26.954475559460036 34.0 26.0 35.0 2.0 35.0 94-95 26.8300185852268 34.0 25.0 35.0 2.0 35.0 96-97 26.705860690553557 34.0 25.0 35.0 2.0 35.0 98-99 26.60493342307734 34.0 25.0 35.0 2.0 35.0 100-101 26.492621797885054 34.0 25.0 35.0 2.0 35.0 102-103 25.231837713992935 31.5 21.5 34.0 2.0 35.0 104-105 26.145134851650496 33.0 24.0 35.0 2.0 35.0 106-107 26.298785314045624 33.5 24.0 35.0 2.0 35.0 108-109 26.28339782173408 34.0 24.0 35.0 2.0 35.0 110-111 26.191579792614004 34.0 24.0 35.0 2.0 35.0 112-113 26.09799598568768 34.0 24.0 35.0 2.0 35.0 114-115 25.980038403023904 33.5 23.5 35.0 2.0 35.0 116-117 25.845630840359185 33.0 23.0 35.0 2.0 35.0 118-119 25.69715717325709 33.0 21.5 35.0 2.0 35.0 120-121 25.49010560227373 33.0 19.0 35.0 2.0 35.0 122-123 25.183880155497203 33.0 9.5 35.0 2.0 35.0 124-125 23.4216015801068 31.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7935605971359401 1101 2 0.9180431801416802 1101 3 0.7941575871153788 1101 4 0.6760085507307707 1101 5 0.8385270594272711 1101 6 0.8194694237841702 1101 7 1.0142703908285498 1101 8 0.9677516253902851 1101 9 1.1413866731693147 1101 10-11 1.1570989180536841 1101 12-13 1.127139104764197 1101 14-15 1.2206183687167425 1101 16-17 1.246308330019538 1101 18-19 1.2983761013432797 1101 20-21 1.288226796257952 1101 22-23 1.2354028274484747 1101 24-25 1.1979921520077141 1101 26-27 1.291709063115455 1101 28-29 1.3126873229798832 1101 30-31 1.3805366643032428 1101 32-33 1.4358840137332507 1101 34-35 1.5461920156965192 1101 36-37 1.5691851601301892 1101 38-39 1.4628627141235313 1101 40-41 1.3325225152703837 1101 42-43 1.3428676196787563 1101 44-45 1.4419454064631694 1101 46-47 1.3868282117433708 1101 48-49 1.5821524552731177 1101 50-51 1.529745997074862 1101 52-53 1.5229487341446664 1101 54-55 1.568056180736761 1101 56-57 1.6689671986898773 1101 58-59 1.6597621550982353 1101 60-61 1.5778216987012534 1101 62-63 1.6343295876696828 1101 64-65 1.4121210435829745 1101 66-67 1.5716491734550253 1101 68-69 1.5669084438997132 1101 70-71 1.614008341208752 1101 72-73 1.554366506424934 1101 74-75 1.6230650683765866 1101 76-77 1.5984266008204173 1101 78-79 1.6144610283377965 1101 80-81 1.3643385156730758 1101 82-83 1.4825156093112497 1101 84-85 1.670010866322798 1101 86-87 1.6436875990995325 1101 88-89 1.500399540206299 1101 90-91 1.5689405100925171 1101 92-93 1.4952797215778517 1101 94-95 1.4652217141787602 1101 96-97 1.591037453444816 1101 98-99 1.5956693841372669 1101 100-101 1.4931700038512332 1101 102-103 1.3947426618151049 1101 104-105 1.5200094112422136 1101 106-107 1.4040570118714655 1101 108-109 1.3866341299269003 1101 110-111 1.4447212803550826 1101 112-113 1.4971475413106532 1101 114-115 1.4955272564129807 1101 116-117 1.536515572302715 1101 118-119 1.5835165224432792 1101 120-121 1.437553708064911 1101 122-123 1.473455546504308 1101 124-125 1.704156847241439 1102 1 0.336004111067421 1102 2 -0.2816180345053816 1102 3 0.21892921826528422 1102 4 0.3778079793171756 1102 5 0.6963499212133328 1102 6 0.34277811818274273 1102 7 0.31142347986946817 1102 8 0.3281543671270697 1102 9 0.7798440530545108 1102 10-11 0.7853889694284817 1102 12-13 0.7811288429099932 1102 14-15 0.9637858151794845 1102 16-17 0.9407427812790061 1102 18-19 1.031638663268268 1102 20-21 1.0036004103178726 1102 22-23 1.1177750129744126 1102 24-25 0.9283498097362113 1102 26-27 0.9488613985719283 1102 28-29 0.5127505585438286 1102 30-31 0.7771302988082383 1102 32-33 0.8925722394678317 1102 34-35 0.8406759414127478 1102 36-37 0.3289339216305365 1102 38-39 -0.20215753436274042 1102 40-41 -0.4374797454829391 1102 42-43 -0.275346680077476 1102 44-45 -0.6031946291846495 1102 46-47 -0.5092560979732816 1102 48-49 -0.636173407744888 1102 50-51 -0.7642346652930954 1102 52-53 -0.9211929659418381 1102 54-55 -0.7846740884567112 1102 56-57 -0.898121475263622 1102 58-59 -0.7931343941403171 1102 60-61 -1.009175201773175 1102 62-63 -0.9487129603631281 1102 64-65 -0.9263912169082325 1102 66-67 -0.8836021633382423 1102 68-69 -0.8816926740264215 1102 70-71 -1.4227544566209218 1102 72-73 -1.1109759813211255 1102 74-75 -1.0077108287165437 1102 76-77 -1.2871045779167076 1102 78-79 -1.0887998625956001 1102 80-81 -1.1945842517511025 1102 82-83 -1.405083503424983 1102 84-85 -1.319336398493327 1102 86-87 -1.0178465022346366 1102 88-89 -1.1177172850407473 1102 90-91 -0.9903849335393637 1102 92-93 -0.8883932162219423 1102 94-95 -0.7535769411644822 1102 96-97 -0.9053466518211017 1102 98-99 -1.0335683815831587 1102 100-101 -0.8174044240798821 1102 102-103 -0.8126460698039857 1102 104-105 -0.8424103156325735 1102 106-107 -0.8413412083586707 1102 108-109 -0.7660787740598884 1102 110-111 -0.6389664014881227 1102 112-113 -0.4822589617954236 1102 114-115 -0.548067275840431 1102 116-117 -0.6537009357835721 1102 118-119 -0.4813482582964177 1102 120-121 -0.26254229203993873 1102 122-123 -0.34067006619260454 1102 124-125 -0.025822871507738654 1103 1 0.3679969155818519 1103 2 -0.14661889697796582 1103 3 0.27362969385730906 1103 4 0.29117630439541387 1103 5 0.3802512543495311 1103 6 0.38554936581094523 1103 7 0.4828238889278893 1103 8 0.5976647291191384 1103 9 0.4749057814495714 1103 10-11 0.6704805147329296 1103 12-13 0.6934375588115245 1103 14-15 0.7671802608560299 1103 16-17 0.6975489220838185 1103 18-19 0.8108136466348626 1103 20-21 0.840870314953051 1103 22-23 0.7979467682293375 1103 24-25 0.6290952324709949 1103 26-27 0.6354881450789094 1103 28-29 0.6066972330959644 1103 30-31 0.7062757116861818 1103 32-33 0.7492342241404657 1103 34-35 0.794759133054157 1103 36-37 0.8442366495081188 1103 38-39 0.8299042679585398 1103 40-41 0.6504148357250159 1103 42-43 0.7796729103712607 1103 44-45 0.6342835009747816 1103 46-47 0.7459470759258338 1103 48-49 0.6020871082601431 1103 50-51 0.5222750313629732 1103 52-53 0.5564702261924133 1103 54-55 0.6151792912037237 1103 56-57 0.5898051406403724 1103 58-59 0.6410811254378146 1103 60-61 0.6431066542678288 1103 62-63 0.6511736860970387 1103 64-65 0.5473452607537261 1103 66-67 0.6401909910936325 1103 68-69 0.588347492554 1103 70-71 0.49565317256514874 1103 72-73 0.6138841749096215 1103 74-75 0.6961530484673588 1103 76-77 0.7394718987595681 1103 78-79 0.5809014754690089 1103 80-81 0.6871719888541037 1103 82-83 0.6463826999706974 1103 84-85 0.6800746559410129 1103 86-87 0.765995689924253 1103 88-89 0.878474282441573 1103 90-91 0.8018959368186884 1103 92-93 0.6871482933515018 1103 94-95 0.6878069577044492 1103 96-97 0.614619588537689 1103 98-99 0.6477270522405476 1103 100-101 0.6247002554499446 1103 102-103 0.45543258769582806 1103 104-105 0.4636282245709715 1103 106-107 0.6747365127423386 1103 108-109 0.6709915297768809 1103 110-111 0.6663267159806487 1103 112-113 0.6465574793962681 1103 114-115 0.7228747906438855 1103 116-117 0.6348380356562622 1103 118-119 0.43642606959101826 1103 120-121 0.4743185541688817 1103 122-123 0.4444658348448236 1103 124-125 0.7692585705464232 1104 1 0.36019731011442246 1104 2 0.6240783051090872 1104 3 0.349316915904442 1104 4 0.08220477377245317 1104 5 0.3829429469876473 1104 6 0.58802394880869 1104 7 0.6558462652279218 1104 8 0.4787283329057175 1104 9 0.6971280036717928 1104 10-11 0.6509943408818089 1104 12-13 0.5758656850152519 1104 14-15 0.8058152715364244 1104 16-17 0.8269567730485505 1104 18-19 0.772769267556761 1104 20-21 0.6806959268870685 1104 22-23 0.590365439658413 1104 24-25 0.805098780929633 1104 26-27 0.7876528215738787 1104 28-29 0.856144537317924 1104 30-31 0.7860646717777016 1104 32-33 0.743406114278315 1104 34-35 1.0197640938927108 1104 36-37 1.0827350912211813 1104 38-39 1.1969327840258188 1104 40-41 1.1919224637438361 1104 42-43 1.1299489664462428 1104 44-45 0.9283410000117911 1104 46-47 0.9905922884248142 1104 48-49 0.9111625246787654 1104 50-51 0.9115829652028964 1104 52-53 0.9225042400360657 1104 54-55 0.641695848731942 1104 56-57 0.7751187239999737 1104 58-59 0.8095080143500439 1104 60-61 0.8682668601718113 1104 62-63 0.9841871912504736 1104 64-65 0.9941849147790087 1104 66-67 1.1105823720743615 1104 68-69 0.9033408975568591 1104 70-71 0.46925334065003454 1104 72-73 0.409372729963259 1104 74-75 0.7444225912531337 1104 76-77 0.7054230374179262 1104 78-79 0.8381332108287012 1104 80-81 0.8005966016168813 1104 82-83 0.7962268712772058 1104 84-85 0.7547160164947613 1104 86-87 0.814490513143312 1104 88-89 0.8713773651650136 1104 90-91 0.9319130954838144 1104 92-93 0.859917375071106 1104 94-95 0.8752729613696317 1104 96-97 0.8332606689499613 1104 98-99 0.9203735720663957 1104 100-101 0.9366891431715807 1104 102-103 0.7517463928340895 1104 104-105 0.6944426191735786 1104 106-107 0.7714051637444221 1104 108-109 0.8176444228212034 1104 110-111 0.9455040338595104 1104 112-113 0.7972082246893066 1104 114-115 0.872118992299054 1104 116-117 0.8483350182991636 1104 118-119 0.8539127336897216 1104 120-121 0.8658419401454651 1104 122-123 0.9300035313815762 1104 124-125 1.3249460260447812 1105 1 0.3365230294982169 1105 2 0.029840097259263842 1105 3 0.267226043477816 1105 4 0.3361177694277302 1105 5 0.05545695579929344 1105 6 0.5085530449729987 1105 7 0.638987111072943 1105 8 0.670657301967168 1105 9 0.824513931919789 1105 10-11 0.7436508668187329 1105 12-13 0.7203094420043783 1105 14-15 0.8473536132108848 1105 16-17 0.7544080095515184 1105 18-19 0.798222606145309 1105 20-21 0.8910380046520174 1105 22-23 0.8915043239642557 1105 24-25 0.7117892488269959 1105 26-27 0.7725552334726586 1105 28-29 0.8214315575504969 1105 30-31 0.8972433062775664 1105 32-33 0.9769895285786134 1105 34-35 1.064557154565989 1105 36-37 1.074410236503411 1105 38-39 1.1028054092496262 1105 40-41 0.790213373445809 1105 42-43 0.8328536179216712 1105 44-45 1.0175848449952056 1105 46-47 0.964430641709626 1105 48-49 1.0416546815774552 1105 50-51 0.8179843963320934 1105 52-53 0.9806651748530903 1105 54-55 1.0000291056813495 1105 56-57 0.8612555001095217 1105 58-59 0.5029293725173218 1105 60-61 0.6727023218668009 1105 62-63 0.8913672801002548 1105 64-65 0.7281495612436579 1105 66-67 0.8633593096289793 1105 68-69 0.8805172283175828 1105 70-71 0.9447118733454047 1105 72-73 0.9586897049154395 1105 74-75 0.8367397434160218 1105 76-77 0.8957430555056405 1105 78-79 0.7817901370173885 1105 80-81 0.859977005779502 1105 82-83 0.865073687900793 1105 84-85 0.8204311686209849 1105 86-87 0.8783343355170778 1105 88-89 0.8170089685552391 1105 90-91 0.6739486965030785 1105 92-93 0.8011613331027192 1105 94-95 0.7121405788633837 1105 96-97 0.8316929652951437 1105 98-99 0.7686609681078735 1105 100-101 0.8362717050272792 1105 102-103 0.42927408964490965 1105 104-105 0.621212663842897 1105 106-107 0.6433323148684913 1105 108-109 0.6370197398144875 1105 110-111 0.5866063760961531 1105 112-113 0.5058031729621142 1105 114-115 0.5609536486019486 1105 116-117 0.3282525389380986 1105 118-119 0.5058722868743217 1105 120-121 0.5274082994432234 1105 122-123 0.5914453084993987 1105 124-125 0.8980556003113449 1106 1 0.08296057543649127 1106 2 -0.28789066206502767 1106 3 0.1595924377082838 1106 4 0.008611974138091227 1106 5 0.3614199790244328 1106 6 0.5880645758717833 1106 7 0.819844437010083 1106 8 0.6819714663460843 1106 9 0.8103767233333414 1106 10-11 0.7408867963580619 1106 12-13 0.7633154383230405 1106 14-15 0.7486365866813358 1106 16-17 0.579350449289926 1106 18-19 0.8229768952878516 1106 20-21 0.7771660601360253 1106 22-23 0.6954047580612936 1106 24-25 0.7534473298793856 1106 26-27 0.8391085595605716 1106 28-29 1.0260805106290718 1106 30-31 0.906365312044592 1106 32-33 0.8431853999451704 1106 34-35 0.8597465930039974 1106 36-37 0.9600851129019787 1106 38-39 0.8766024547512856 1106 40-41 0.8135098881052301 1106 42-43 0.7393951492602113 1106 44-45 0.5211734485345687 1106 46-47 0.5467810792618479 1106 48-49 0.6127231508043067 1106 50-51 0.6484891362193892 1106 52-53 0.5331985331056366 1106 54-55 0.5709899144462156 1106 56-57 0.15464620000460982 1106 58-59 0.5902785222274005 1106 60-61 0.5581343143784672 1106 62-63 0.4857642244591922 1106 64-65 0.6537484863666272 1106 66-67 0.5699803102509087 1106 68-69 0.5362460841483667 1106 70-71 0.4905239520482674 1106 72-73 0.6072757484759137 1106 74-75 0.49205743208489494 1106 76-77 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2216 90-91 -1.669152723716067 2216 92-93 -1.6882026683111242 2216 94-95 -1.5890256986095395 2216 96-97 -1.6879505023341714 2216 98-99 -1.8369453773918707 2216 100-101 -1.7708000563110033 2216 102-103 -1.7027991927359132 2216 104-105 -1.624493867658618 2216 106-107 -1.610839470227031 2216 108-109 -1.7140181182099532 2216 110-111 -1.73269999953472 2216 112-113 -1.6153412739288768 2216 114-115 -1.7716275171250508 2216 116-117 -1.6840532022007402 2216 118-119 -1.6389241541758643 2216 120-121 -1.8143351919433321 2216 122-123 -1.8103041077099924 2216 124-125 -2.046960377878353 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 57977.0 3 19303.0 4 9432.0 5 7655.0 6 8857.0 7 10417.0 8 11538.0 9 12078.0 10 13375.0 11 14738.0 12 16399.0 13 18775.0 14 22704.0 15 19360.0 16 12553.0 17 10839.0 18 10434.0 19 9590.0 20 9397.0 21 9615.0 22 10714.0 23 12977.0 24 16791.0 25 21897.0 26 27103.0 27 29482.0 28 33669.0 29 39597.0 30 45697.0 31 53230.0 32 64208.0 33 79171.0 34 102704.0 35 143667.0 36 217917.0 37 322944.0 38 127648.0 39 626.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.44825542435656 19.295081610288197 13.942029721772661 23.31463324358258 2 15.382477442150764 21.290114423610245 39.40376223960442 23.923645894634575 3 18.574109498162624 26.596812959872583 29.825120024554735 25.003957517410058 4 12.227260960970405 17.555273579724837 36.61585860027628 33.60160685902848 5 12.513972151161457 39.291259988955204 33.543977987744384 14.650789872138956 6 30.596866129572142 37.76112062392226 17.254413387163623 14.387599859341977 7 27.174640732022098 34.23782742569328 20.773374774626713 17.814157067657906 8 26.36527989597407 35.303135037934176 19.38975402604999 18.941831040041766 9 27.040929156752302 15.642541477191019 19.673100883017945 37.64342848303873 10-11 24.74784230593895 27.38112177433461 26.856822282864336 21.014213636862106 12-13 25.94729674371842 24.885322625278082 27.92505851687957 21.242322114123926 14-15 22.67516858732252 25.60095391332246 26.950105644631638 24.773771854723375 16-17 21.313669358590612 29.088669147119706 27.39511949314653 22.20254200114315 18-19 20.978664576889635 26.28871429771226 31.460821816498807 21.271799308899297 20-21 22.238559415993826 25.55749037340843 31.38317187808714 20.8207783325106 22-23 21.576566179962516 25.635589380077555 31.31230068915181 21.475543750808118 24-25 21.281716027885093 25.67906165147504 31.728776528961173 21.3104457916787 26-27 21.27244154051954 26.138254511267746 31.448276153752268 21.14102779446044 28-29 21.02716609126579 25.925062142086354 31.838469244349817 21.209302522298042 30-31 21.226461834427138 25.81603404794215 31.72249283719559 21.23501128043512 32-33 21.16262798490464 25.84310225862467 31.498273797367858 21.495995959102835 34-35 21.42515337645718 25.86926416761023 31.279281097325928 21.426301358606665 36-37 21.69163628541978 25.673170690444802 31.186083072821948 21.44910995131347 38-39 21.36431032253465 25.78787827522328 31.311515227681113 21.536296174560956 40-41 21.351229368041867 25.8023186822615 31.317647869163874 21.52880408053276 42-43 21.683691040543103 25.75760779854484 30.80021002031324 21.758491140598814 44-45 21.52333606029444 26.081187714415876 30.411980583392445 21.983495641897242 46-47 21.704566189629734 26.353350114012752 29.762162266672625 22.179921429684885 48-49 22.034308957040093 26.19595571930749 29.555555689822473 22.214179633829946 50-51 22.040968486662553 26.62078561903766 29.026685767489973 22.31156012680981 52-53 22.154665822396286 26.734329137357875 28.814140481596638 22.296864558649197 54-55 21.828940992509114 26.575484659937477 28.995521661214756 22.60005268633865 56-57 21.92050375057555 26.991273431278117 28.786981569187155 22.30124124895918 58-59 22.01902101096362 26.42241435303579 29.036360818566287 22.522203817434306 60-61 22.16795824728502 26.492255954100045 29.24375769601191 22.096028102603018 62-63 21.868878687288454 26.65934777696278 29.089142626510657 22.382630909238113 64-65 21.80852831442684 26.34448589943054 29.208857604640272 22.63812818150235 66-67 21.95848171506116 26.81284507437112 28.81018296418658 22.418490246381136 68-69 21.950784781981508 26.47422854820998 29.098395694461438 22.476590975347076 70-71 21.848758789615957 26.56303811663257 29.065548572333142 22.522654521418325 72-73 21.95038541990166 26.60478841480583 28.78435336582324 22.660472799469268 74-75 21.78771152249065 26.85857172679208 28.58195908352263 22.771757667194645 76-77 21.904345293696128 26.774025151684693 28.6664737251054 22.655155829513777 78-79 21.87063087056924 26.74281816325273 28.74622827443782 22.64032269174021 80-81 21.611031759266645 27.284759128885927 28.59391351212875 22.51029559971868 82-83 21.88422539602363 27.02056942331419 28.311022199557968 22.784182981104216 84-85 21.96379868501666 26.707744287580404 28.21773354488429 23.11072348251865 86-87 21.916459526378816 26.408332416961617 28.5011038754669 23.17410418119267 88-89 21.857242853789497 26.474912774828397 28.613813856997545 23.054030514384564 90-91 21.756134756186718 26.81347948555899 28.55113172913905 22.879254029115245 92-93 21.695549273035645 26.446572364418113 28.609429540075638 23.24844882247061 94-95 21.717767984348775 26.561044252899258 28.46542579866326 23.255761964088702 96-97 21.751563750535905 26.585315081041905 28.459126765082566 23.203994403339625 98-99 21.778628804696574 26.500156496035636 28.313214428571946 23.408000270695844 100-101 21.554136848440024 26.533931752212048 28.504502642855588 23.407428756492337 102-103 21.791238841915607 26.153268909380706 28.610585120459582 23.444907128244107 104-105 21.660157406478728 26.292174749468 28.467691552905666 23.57997629114761 106-107 21.692311921788445 26.46274325934058 28.391930271571937 23.453014547299038 108-109 21.85852324135879 26.42867176914676 28.008325894104658 23.704479095389793 110-111 21.51799117321234 26.55162861150443 28.265991491022614 23.664388724260615 112-113 21.531402145397376 26.371808458574158 28.17181425890502 23.924975137123447 114-115 21.183321873651874 26.516514629521993 28.227944544003364 24.072218952822766 116-117 21.238455226883566 26.487512975219296 28.291838813638993 23.982192984258145 118-119 21.300687943408107 26.51264774228163 27.998529374446402 24.18813493986386 120-121 21.23329593289915 26.50588105211832 28.091049212083423 24.16977380289911 122-123 21.264839568759374 26.546615770162102 27.80767888676233 24.3808657743162 124-125 21.18798314612177 26.52683849495448 27.73594341083021 24.549234948093538 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1236.0 1 931.0 2 1267.0 3 1870.0 4 1255.5 5 824.0 6 1703.5 7 4144.5 8 6546.5 9 6890.5 10 4851.5 11 2592.5 12 1965.0 13 1918.5 14 1994.0 15 2176.5 16 2439.0 17 2720.0 18 3015.0 19 3295.0 20 3708.0 21 4261.0 22 4918.5 23 5686.0 24 6602.5 25 8070.0 26 10106.5 27 12507.5 28 14952.0 29 17432.0 30 20365.0 31 23785.0 32 27390.0 33 30883.5 34 34402.0 35 37015.0 36 39882.5 37 43429.5 38 45009.0 39 45560.0 40 46385.5 41 49261.5 42 52620.0 43 54377.0 44 55636.5 45 56916.0 46 57043.5 47 56381.0 48 59562.5 49 60524.0 50 57648.0 51 55903.5 52 55173.5 53 54615.0 54 52950.0 55 48734.5 56 42758.0 57 38034.5 58 35362.0 59 32738.0 60 28858.0 61 24825.5 62 20701.0 63 15838.0 64 11844.5 65 9354.0 66 7175.5 67 6057.0 68 4396.5 69 2644.0 70 2099.5 71 1937.5 72 1775.5 73 1546.0 74 1307.0 75 1131.5 76 782.5 77 412.5 78 236.0 79 148.5 80 125.0 81 95.5 82 55.0 83 40.5 84 28.0 85 16.0 86 14.5 87 7.5 88 5.0 89 5.0 90 2.5 91 2.0 92 2.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09727638214029792 2 0.0 3 0.0 4 0.10065990847561264 5 0.0 6 0.0 7 0.002779325204008512 8 0.006404531991845702 9 0.004350248145404627 10-11 7.552514141327478E-4 12-13 0.0 14-15 6.042011313061982E-5 16-17 2.416804525224793E-4 18-19 0.0022355441858329336 20-21 0.0016313430545267353 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 6.042011313061982E-5 52-53 0.0 54-55 0.0 56-57 0.007975454933241817 58-59 8.156715272633676E-4 60-61 0.0 62-63 0.0 64-65 1.8126033939185946E-4 66-67 0.0 68-69 3.021005656530991E-5 70-71 0.0 72-73 0.0 74-75 0.0014198726585695658 76-77 0.0 78-79 0.0 80-81 1.2084022626123965E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.001359452545438946 90-91 0.0 92-93 0.002718905090877892 94-95 0.0 96-97 0.009818268383725723 98-99 0.005075289502972065 100-101 0.0030210056565309912 102-103 0.0 104-105 0.0 106-107 0.001721973224222665 108-109 3.021005656530991E-5 110-111 1.5105028282654955E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 0.001027141923220537 122-123 0.005165919672667995 124-125 0.08060043091624684 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1655078.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.11399663634764 #Duplication Level Percentage of deduplicated Percentage of total 1 74.05126242520498 36.369534536688136 2 12.432357356780384 12.212055148055672 3 5.168725167004527 7.615702513993973 4 2.633877365620984 5.174409763026448 5 1.5089882839126858 3.705622275018783 6 0.989372617551095 2.9155226046299365 7 0.6448202001536373 2.216878799897633 8 0.45054672611304575 1.7702520312666856 9 0.3387095602991598 1.497184218470855 >10 1.6152421130896346 13.551261129849735 >50 0.09934732829170598 3.392146463318471 >100 0.061559331604782205 5.621284440686738 >500 0.003090193079399371 0.9418953719402136 >1k 0.0017305081244636477 1.313796825276066 >5k 2.4721544635194966E-4 0.9987187085909413 >10k+ 1.2360772317597483E-4 0.7037351692897812 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11592 0.700389951410145 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9166 0.5538107569552613 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7285 0.4401605241565654 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2584 0.15612557232952162 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2145 0.12960114266517953 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2118 0.1279697996106528 No Hit TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC 1928 0.11648997811583503 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 1786 0.107910322051287 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 1756 0.1060977186573684 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1743 0.10531225718667037 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 6.042011313061982E-5 0.0 0.0 0.0 0.0 6 6.042011313061982E-5 0.0 0.0 0.0 0.0 7 6.042011313061982E-5 0.0 0.0 0.0 0.0 8 6.042011313061982E-5 0.0 0.0 0.0 0.0 9 6.042011313061982E-5 0.0 0.0 0.0 0.0 10-11 1.2084022626123965E-4 0.0 0.0 9.063016969592973E-5 0.0 12-13 1.2084022626123965E-4 0.0 0.0 1.2084022626123965E-4 0.0 14-15 1.812603393918595E-4 0.0 0.0 1.2084022626123965E-4 0.0 16-17 1.812603393918595E-4 0.0 0.0 1.5105028282654957E-4 0.0 18-19 2.4168045252247932E-4 0.0 0.0 3.0210056565309915E-4 0.0 20-21 3.0210056565309915E-4 0.0 0.0 4.531508484796487E-4 0.0 22-23 3.0210056565309915E-4 0.0 0.0 0.001087562036351157 0.0 24-25 4.229407919143388E-4 0.0 0.0 0.0019032335636145247 0.0 26-27 4.833609050449586E-4 0.0 0.0 0.002869955373704442 0.0 28-29 4.833609050449586E-4 0.0 0.0 0.003806467127229049 0.0 30-31 4.833609050449586E-4 0.0 0.0 0.008005664989807128 0.0 32-33 4.833609050449586E-4 0.0 0.0 0.015830069640222395 0.0 34-35 5.135709616102685E-4 0.0 0.0 0.02525560728859909 0.0 36-37 6.042011313061983E-4 0.0 0.0 0.04123672721164803 0.0 38-39 7.854614706980578E-4 0.0 0.0 0.06936228987395156 0.0 40-41 7.854614706980578E-4 0.0 0.0 0.10274440237861901 0.0 42-43 8.156715272633677E-4 0.0 0.0 0.1349785327338047 0.0 44-45 8.458815838286776E-4 0.0 0.0 0.17011282851926013 0.0 46-47 0.0010573519797858469 0.0 0.0 0.21431014127430853 0.0 48-49 0.0011479821494817766 0.0 0.0 0.2633108530232412 0.0 50-51 0.0011479821494817766 0.0 0.0 0.31802126546301746 0.0 52-53 0.0011479821494817766 0.0 0.0 0.37161390580987724 0.0 54-55 0.0011479821494817766 0.0 0.0 0.4311881373566684 0.0 56-57 0.0012084022626123966 0.0 0.0 0.5018192496063629 0.0 58-59 0.0012084022626123966 0.0 0.0 0.5867397186114491 0.0 60-61 0.0012084022626123966 0.0 0.0 0.6828076984891347 0.0 62-63 0.0012084022626123966 0.0 0.0 0.7779089565567303 0.0 64-65 0.0012084022626123966 0.0 0.0 0.8670286234243945 0.0 66-67 0.0012084022626123966 0.0 0.0 0.9744253745140713 0.0 68-69 0.0012688223757430163 0.0 0.0 1.1001294198823257 0.0 70-71 0.0012990324323083262 0.0 0.0 1.2486118479008241 0.0 72-73 0.0013896626020042562 0.0 0.0 1.3986349888041532 0.0 74-75 0.001450082715134876 0.0 0.0 1.54944359117818 0.0 76-77 0.001450082715134876 0.0 0.0 1.7152061715520355 0.0 78-79 0.0014802927717001858 0.0 0.0 1.9099704062285885 0.0 80-81 0.0016313430545267353 0.0 0.0 2.1282984850260833 0.0 82-83 0.0016917631676573552 0.0 0.0 2.360341929504229 0.0 84-85 0.0017823933373532848 6.042011313061982E-5 0.0 2.5935937762449868 0.0 86-87 0.0018126033939185949 6.042011313061982E-5 0.0 2.831195871131149 0.0 88-89 0.0018126033939185949 6.042011313061982E-5 0.0 3.101968608126022 0.0 90-91 0.0019334436201798343 6.042011313061982E-5 0.0 3.4143708030678916 0.0 92-93 0.002054283846441074 6.042011313061982E-5 0.0 3.753508898070061 0.0 94-95 0.002054283846441074 6.042011313061982E-5 0.0 4.092858463468187 0.0 96-97 0.002054283846441074 6.042011313061982E-5 0.0 4.435440504918802 0.0 98-99 0.0022053341292676237 6.042011313061982E-5 0.0 4.799924837379265 0.0 100-101 0.0022959642989635533 6.042011313061982E-5 0.0 5.224708442744088 0.0 102-103 0.002356384412094173 6.042011313061982E-5 0.0 5.686650417684242 0.0 104-105 0.002356384412094173 6.042011313061982E-5 0.0 6.152126969242538 0.0 106-107 0.0025074346949207228 6.042011313061982E-5 0.0 6.609597855811025 0.0 108-109 0.0025980648646166523 6.042011313061982E-5 0.0 7.087309480278271 0.0 110-111 0.0026584849777472725 6.042011313061982E-5 0.0 7.632449950999288 0.0 112-113 0.0026584849777472725 6.042011313061982E-5 0.0 8.228222476523765 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5480 0.0 59.280968 1 GTATCAA 9965 0.0 51.143227 1 ATCAACG 12975 0.0 39.24434 3 TATCAAC 13160 0.0 38.421127 2 TCAACGC 13235 0.0 38.2034 4 CAACGCA 13375 0.0 37.63352 5 AACGCAG 13680 0.0 36.837955 6 GTACATG 9980 0.0 36.254128 1 TACATGG 9880 0.0 35.865616 2 ACATGGG 10300 0.0 33.53583 3 ACGCAGA 15655 0.0 31.962528 7 CGCAGAG 15685 0.0 31.93933 8 GCAGAGT 16475 0.0 29.61329 9 CATGGGA 7055 0.0 29.54536 4 GAGTACT 10730 0.0 28.722898 12-13 GACGGGT 105 5.908251E-4 28.33211 8 AGTACTT 11065 0.0 27.369354 12-13 CATGGGG 3665 0.0 27.29943 4 ATGGGAG 2690 0.0 26.983965 5 CGCGATT 45 0.009534908 26.442505 36-37 >>END_MODULE