FastQCFastQC Report
Thu 26 May 2016
SRR1364588_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364588_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2473422
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37870.1531077187798928No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27520.11126285769270267No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42500.053.0572661
GTATCAA67150.049.883511
ATCAACG80100.041.2236373
GTACATG111550.040.0024221
CAACGCA82850.039.71175
TCAACGC83400.039.3071334
AACGCAG84500.039.002736
TATCAAC88100.037.615352
TACATGG116650.037.1306422
ACATGGG119300.035.308453
ACGCAGA99300.032.7702677
CGCAGAG99950.031.727738
CATGGGA67750.029.506374
CATGGGG57750.028.1252564
GCAGAGT108850.027.7676229
ATGGGAG33750.025.2085635
GAGTACT54650.024.93529912-13
CAGAGTA106300.024.48993910-11
GAGTACA82350.024.2756181
AGAGTAC98750.023.4398810-11
Produced by FastQC (version 0.11.5)