##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364588_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2473422 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.833284413254187 31.0 28.0 33.0 15.0 34.0 2 27.704855459359543 31.0 28.0 33.0 12.0 34.0 3 27.945194552324676 31.0 28.0 34.0 16.0 34.0 4 31.225554717310672 35.0 32.0 37.0 16.0 37.0 5 31.362309383518056 35.0 32.0 37.0 16.0 37.0 6 31.307027268294693 35.0 32.0 37.0 16.0 37.0 7 31.112084391583807 35.0 32.0 37.0 11.0 37.0 8 31.069941158443648 35.0 32.0 37.0 10.0 37.0 9 32.11683287364631 37.0 32.0 39.0 10.0 39.0 10-11 32.009662524227565 37.0 31.5 39.0 10.0 39.0 12-13 31.921185103067735 37.0 32.0 39.0 8.0 39.0 14-15 32.58263996196362 38.0 31.0 40.0 8.0 41.0 16-17 32.682830911991566 38.0 31.0 40.0 8.0 41.0 18-19 32.47820529614437 38.0 31.0 40.0 7.5 41.0 20-21 32.19138869145661 38.0 30.5 40.0 4.5 41.0 22-23 32.08413384371935 38.0 30.0 40.0 2.0 41.0 24-25 31.90235471342941 38.0 30.0 40.0 2.0 41.0 26-27 31.808953749097405 38.0 30.0 40.0 2.0 41.0 28-29 31.864006829404765 38.0 30.0 40.0 2.0 41.0 30-31 31.79399087579879 38.0 30.0 40.0 2.0 41.0 32-33 31.783987528209906 38.0 30.0 40.0 2.0 41.0 34-35 31.29560382336698 37.0 30.0 40.0 2.0 41.0 36-37 31.153926018285595 37.0 29.5 40.0 2.0 41.0 38-39 30.94220436302418 37.0 29.0 40.0 2.0 41.0 40-41 31.154865405094643 37.0 29.5 40.0 2.0 41.0 42-43 31.0279177997123 37.0 29.0 40.0 2.0 41.0 44-45 30.8643201604902 37.0 29.0 40.0 2.0 41.0 46-47 30.814744714003513 37.0 29.0 40.0 2.0 41.0 48-49 30.659630867680484 37.0 29.0 40.0 2.0 41.0 50-51 30.495062104242624 36.5 28.0 40.0 2.0 41.0 52-53 30.436105727207085 36.5 28.0 40.0 2.0 41.0 54-55 30.456414433121402 36.0 28.0 40.0 2.0 41.0 56-57 30.329676658491756 36.0 28.0 40.0 2.0 41.0 58-59 30.12995416875891 36.0 28.0 40.0 2.0 41.0 60-61 29.930359437249287 35.5 28.0 40.0 2.0 41.0 62-63 29.642578581414735 35.0 28.0 39.0 2.0 41.0 64-65 29.35984902697558 35.0 27.5 39.0 2.0 41.0 66-67 29.118340299390884 35.0 27.0 39.0 2.0 40.0 68-69 29.084987923613518 35.0 28.0 38.5 2.0 40.0 70-71 28.859234493749955 35.0 27.5 38.0 2.0 40.0 72-73 28.581281722245535 34.5 27.0 37.0 2.0 40.0 74-75 28.28324200237566 34.0 26.0 37.0 2.0 39.0 76-77 27.98535551151401 34.0 26.0 36.0 2.0 39.0 78-79 27.680488206217944 34.0 26.0 36.0 2.0 38.5 80-81 27.381100758382516 34.0 26.0 35.0 2.0 37.0 82-83 27.13863808925448 34.0 26.0 35.0 2.0 37.0 84-85 26.899897591272335 34.0 25.5 35.0 2.0 36.5 86-87 26.70701036863099 34.0 25.0 35.0 2.0 36.0 88-89 26.511825519462512 33.0 25.0 35.0 2.0 36.0 90-91 26.37568862086615 33.0 24.5 35.0 2.0 35.5 92-93 26.252162793085855 33.0 24.0 35.0 2.0 35.0 94-95 26.1294146328447 33.0 24.0 35.0 2.0 35.0 96-97 26.011417380455093 33.0 23.5 35.0 2.0 35.0 98-99 25.92650849713474 33.0 23.0 35.0 2.0 35.0 100-101 25.833517895450107 33.0 23.0 35.0 2.0 35.0 102-103 24.711235082408095 31.5 19.5 34.0 2.0 35.0 104-105 25.557477656461373 33.0 21.5 35.0 2.0 35.0 106-107 25.697820671118798 33.0 23.0 35.0 2.0 35.0 108-109 25.661908077149796 33.0 21.0 35.0 2.0 35.0 110-111 25.581312448906818 33.0 20.0 35.0 2.0 35.0 112-113 25.490715696714915 33.0 19.5 35.0 2.0 35.0 114-115 25.36875996089628 33.0 19.0 35.0 2.0 35.0 116-117 25.262912677254427 33.0 18.0 35.0 2.0 35.0 118-119 25.11623754458398 33.0 16.0 35.0 2.0 35.0 120-121 24.939724195871143 33.0 4.5 35.0 2.0 35.0 122-123 24.701546076650082 33.0 2.0 35.0 2.0 35.0 124-125 23.294590854290128 31.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8835169562361962 1101 2 0.9156404163042531 1101 3 0.8815999315360408 1101 4 0.944144681039969 1101 5 0.8934682954810071 1101 6 1.0065231919945639 1101 7 0.9916605895156252 1101 8 0.9626191264317043 1101 9 1.3028887913103233 1101 10-11 1.3844712664267576 1101 12-13 1.2103681740073178 1101 14-15 1.53187243388453 1101 16-17 1.255561951147726 1101 18-19 1.5939466309435701 1101 20-21 1.9763880358858472 1101 22-23 1.8820249968188847 1101 24-25 1.9690152338617892 1101 26-27 2.1170921952526385 1101 28-29 1.9375097271406538 1101 30-31 2.0607731839246064 1101 32-33 2.0180403816646333 1101 34-35 2.296910707654689 1101 36-37 2.3323308120700474 1101 38-39 2.0764675709395064 1101 40-41 1.8945968994855278 1101 42-43 1.847945025337637 1101 44-45 1.7789164248397107 1101 46-47 1.6446006148044745 1101 48-49 1.7681684849550052 1101 50-51 1.7018019096252601 1101 52-53 1.498723374399379 1101 54-55 1.4192094331206135 1101 56-57 1.437171165366383 1101 58-59 1.2851184273026668 1101 60-61 1.3244905581879074 1101 62-63 1.2778350213466219 1101 64-65 1.3929058367383433 1101 66-67 1.3002907850954237 1101 68-69 1.1819629280173949 1101 70-71 1.23414128141048 1101 72-73 1.0289543885458414 1101 74-75 1.1434340755907506 1101 76-77 1.3039174199991947 1101 78-79 1.295114542755325 1101 80-81 1.3482243858276597 1101 82-83 1.230154393162671 1101 84-85 1.3101700269484198 1101 86-87 1.2252119322574302 1101 88-89 1.220200018389292 1101 90-91 1.2706099342640194 1101 92-93 1.0506755446088327 1101 94-95 1.1609329587224018 1101 96-97 1.0800927649876222 1101 98-99 1.262021085828266 1101 100-101 1.2535279795572443 1101 102-103 1.1297120890774472 1101 104-105 1.2202129300727727 1101 106-107 1.140731389780047 1101 108-109 1.231073753695135 1101 110-111 0.9763769922572898 1101 112-113 1.0371718397667458 1101 114-115 1.1737867469628114 1101 116-117 1.2196747683511475 1101 118-119 1.2657673905718525 1101 120-121 1.2369953856978526 1101 122-123 1.289828861899469 1101 124-125 1.42032637002929 1102 1 0.010496822008008166 1102 2 0.11466421130119997 1102 3 0.25231565876183737 1102 4 0.34804011446560423 1102 5 0.4414008997894534 1102 6 -0.41766979622527245 1102 7 -0.846296889247224 1102 8 0.05930847734924072 1102 9 0.3596438483338389 1102 10-11 0.31885339436593796 1102 12-13 0.3297918375920581 1102 14-15 0.5498787228975317 1102 16-17 0.44424923701622276 1102 18-19 0.5812043396777895 1102 20-21 0.8882827183845592 1102 22-23 0.7191977569446664 1102 24-25 0.8853281826579646 1102 26-27 0.4728719859317607 1102 28-29 0.9990378634338875 1102 30-31 0.8012753663059833 1102 32-33 0.674151518849591 1102 34-35 1.105750056225581 1102 36-37 1.159280640764841 1102 38-39 1.1707351825225558 1102 40-41 1.003994749957208 1102 42-43 0.7489371875378517 1102 44-45 0.8525316686562938 1102 46-47 0.523406640993052 1102 48-49 0.28411347955772115 1102 50-51 0.7915705302663625 1102 52-53 0.39732665031809233 1102 54-55 0.4163352603135593 1102 56-57 0.5137431839518278 1102 58-59 0.5987393030711949 1102 60-61 0.4532219610132664 1102 62-63 0.30682173932663304 1102 64-65 0.3079778787902505 1102 66-67 0.377530189985837 1102 68-69 0.3063755623629163 1102 70-71 0.18235548331595552 1102 72-73 0.31667861063247926 1102 74-75 0.014140416229974306 1102 76-77 0.2291354234722327 1102 78-79 0.16391540632129065 1102 80-81 -0.15412601544891658 1102 82-83 0.04902143216393995 1102 84-85 -0.2765892540390489 1102 86-87 0.14566248841268603 1102 88-89 0.313598432056164 1102 90-91 -0.08855935282473126 1102 92-93 0.24432458013706437 1102 94-95 0.10920817344056744 1102 96-97 0.23160822938899273 1102 98-99 0.34688896962232363 1102 100-101 0.3512742601223202 1102 102-103 0.10998899338119728 1102 104-105 0.42091880138267257 1102 106-107 0.38633142732639314 1102 108-109 0.039991949593193965 1102 110-111 0.23752591045318994 1102 112-113 -9.245604773049365E-4 1102 114-115 -0.020058468224686266 1102 116-117 -0.1658013662525022 1102 118-119 0.19053859252426264 1102 120-121 0.5187131310397604 1102 122-123 0.3751886028296809 1102 124-125 0.5927044617362434 1103 1 0.7649005166562013 1103 2 0.6947012565705215 1103 3 0.6334238632441256 1103 4 0.6301842566586444 1103 5 0.7957770859504834 1103 6 0.7869206255778671 1103 7 0.909750399514003 1103 8 1.0490290420331263 1103 9 1.3261027195359887 1103 10-11 1.333514658122553 1103 12-13 1.1980786553654248 1103 14-15 1.6768953248774636 1103 16-17 1.4821933195568846 1103 18-19 1.563329294321079 1103 20-21 1.9102249163658769 1103 22-23 1.7266800274587197 1103 24-25 1.7936680036628232 1103 26-27 1.7906648857784475 1103 28-29 1.715481973521026 1103 30-31 1.7529473997873062 1103 32-33 1.7065657495523325 1103 34-35 2.0454001209123263 1103 36-37 1.9641232743605315 1103 38-39 2.068300035490836 1103 40-41 1.7933749161099897 1103 42-43 1.5533313644014655 1103 44-45 1.2463975039891189 1103 46-47 1.4390499273453798 1103 48-49 1.3908600500206703 1103 50-51 1.4536476110231504 1103 52-53 1.4056169636450768 1103 54-55 1.2134046565516599 1103 56-57 1.1860596531387095 1103 58-59 1.055899127761581 1103 60-61 0.9738276036726994 1103 62-63 0.9731435620298718 1103 64-65 1.1093340396085125 1103 66-67 1.1409803773969038 1103 68-69 1.1985962837767907 1103 70-71 1.166706877642902 1103 72-73 1.1940291329707762 1103 74-75 1.1280217915783446 1103 76-77 1.1685118091077875 1103 78-79 1.146344056182702 1103 80-81 1.17260957441626 1103 82-83 1.165331039427091 1103 84-85 1.0966696467993167 1103 86-87 0.9747236093075458 1103 88-89 1.0297709148301166 1103 90-91 1.1511019584258229 1103 92-93 1.1518364488714283 1103 94-95 1.0662552285316345 1103 96-97 1.2653311649701173 1103 98-99 1.1760493718389604 1103 100-101 1.2306129925661828 1103 102-103 1.1946447288437803 1103 104-105 1.1987767869179216 1103 106-107 1.2241559771313852 1103 108-109 1.3271976914199648 1103 110-111 1.40834787848787 1103 112-113 1.458963809777817 1103 114-115 1.515652449491121 1103 116-117 1.5836367425010067 1103 118-119 1.5735886530315355 1103 120-121 1.5474983311697024 1103 122-123 1.615840067637624 1103 124-125 1.7248126035265336 1104 1 0.7332974633762603 1104 2 0.8510463112963436 1104 3 0.6840883542651568 1104 4 0.8487879289173215 1104 5 0.8276200787932382 1104 6 0.7242487021656991 1104 7 0.7576322014376125 1104 8 0.9301724930769666 1104 9 1.2152007660286515 1104 10-11 1.1091549047208886 1104 12-13 1.1052016442471917 1104 14-15 1.1989616335731768 1104 16-17 1.2022669755577482 1104 18-19 1.4271996378056357 1104 20-21 1.5939830842933702 1104 22-23 1.4530098988380473 1104 24-25 1.9005279374729014 1104 26-27 2.081096262990883 1104 28-29 1.8902108272952525 1104 30-31 1.683910507754593 1104 32-33 1.6591696274112593 1104 34-35 1.9252133775114615 1104 36-37 1.9596862800213657 1104 38-39 1.9180939488230635 1104 40-41 1.4494300468890273 1104 42-43 1.5285760628261116 1104 44-45 1.459000218812708 1104 46-47 1.4163333134654152 1104 48-49 1.334256927771719 1104 50-51 1.1819975484646257 1104 52-53 1.278051516045629 1104 54-55 1.045333925998488 1104 56-57 1.0653100137014455 1104 58-59 1.074121974915652 1104 60-61 1.085759663823552 1104 62-63 1.0846421337905348 1104 64-65 1.167993128105337 1104 66-67 1.1812408566574852 1104 68-69 1.1864156665665675 1104 70-71 1.1420894051931185 1104 72-73 1.0841121658533126 1104 74-75 1.1383411166519473 1104 76-77 1.1508804982798395 1104 78-79 1.1258541250050662 1104 80-81 1.0964354060264583 1104 82-83 1.0614092581273908 1104 84-85 1.066496938229399 1104 86-87 1.1421487173093894 1104 88-89 1.188380512615904 1104 90-91 1.1957711910301292 1104 92-93 1.2286926118915211 1104 94-95 1.1546993471519436 1104 96-97 0.9764069421673973 1104 98-99 1.0916294135717486 1104 100-101 1.1717775248960258 1104 102-103 1.12367017056852 1104 104-105 1.1799993200088892 1104 106-107 1.186978775702233 1104 108-109 1.19432780080772 1104 110-111 1.2574284344390882 1104 112-113 1.2437642301548344 1104 114-115 1.2132744415165604 1104 116-117 1.323307072075604 1104 118-119 1.3276568083543978 1104 120-121 1.292025857863976 1104 122-123 1.3027849669145546 1104 124-125 1.6638863584071828 1105 1 0.133757749870707 1105 2 0.5245113553076379 1105 3 0.4626364294365892 1105 4 0.6168009701291126 1105 5 0.5349174127749912 1105 6 0.5698805884932554 1105 7 0.6391778719797507 1105 8 0.4469050695476007 1105 9 0.4528814661732241 1105 10-11 0.5583558388241343 1105 12-13 0.6031066054200984 1105 14-15 0.6784184427646878 1105 16-17 0.6046506644972993 1105 18-19 0.8065115987903582 1105 20-21 1.2680710716242416 1105 22-23 1.3899438839739773 1105 24-25 1.4205853149062477 1105 26-27 1.2941340277247733 1105 28-29 1.0939075954717588 1105 30-31 0.9636744421563854 1105 32-33 1.0349968681905182 1105 34-35 1.5977788533871191 1105 36-37 1.601812563119772 1105 38-39 1.6840656278884012 1105 40-41 1.3222425788245076 1105 42-43 0.9761166747173391 1105 44-45 1.0691770881335714 1105 46-47 1.0810966795680415 1105 48-49 0.9134638931559245 1105 50-51 0.9306216266625924 1105 52-53 0.8730310049604988 1105 54-55 0.6707408547191527 1105 56-57 0.6306386189003597 1105 58-59 0.665204637147145 1105 60-61 0.6623776430893038 1105 62-63 0.7369044589668725 1105 64-65 0.7010393306127654 1105 66-67 0.5881310935070942 1105 68-69 0.3948831020208985 1105 70-71 0.6030490080802764 1105 72-73 0.6156059369934326 1105 74-75 0.5965956011630382 1105 76-77 0.5994128746346412 1105 78-79 0.7235531726847206 1105 80-81 0.6573235379475406 1105 82-83 0.5428659357429559 1105 84-85 0.5533230755192982 1105 86-87 0.5725643181463731 1105 88-89 0.7833651255396745 1105 90-91 0.6304653392973059 1105 92-93 0.5369289079626327 1105 94-95 0.6643827923320025 1105 96-97 0.7451903404932274 1105 98-99 0.6297521831050084 1105 100-101 0.6223046456659773 1105 102-103 0.5790766019378317 1105 104-105 0.6755484195521149 1105 106-107 0.6627800524475838 1105 108-109 0.552465493748155 1105 110-111 0.6684688318385881 1105 112-113 0.7101132512684742 1105 114-115 0.6968027236807544 1105 116-117 0.7722542064933364 1105 118-119 0.8495678228809247 1105 120-121 0.7378739702005959 1105 122-123 1.0161618478737218 1105 124-125 1.2018102232313872 1106 1 0.4316004559245634 1106 2 0.5867846077284611 1106 3 0.4073838476389078 1106 4 0.6258840471143827 1106 5 0.40183723330788723 1106 6 0.3131451761509396 1106 7 0.2436175570063881 1106 8 0.4727790982492017 1106 9 0.6423755446321593 1106 10-11 0.786345041346145 1106 12-13 0.7094787993517038 1106 14-15 0.8646651208871674 1106 16-17 0.7354732485363371 1106 18-19 0.8114064121678837 1106 20-21 1.1770703775625293 1106 22-23 1.1301881662661657 1106 24-25 1.3836822377549538 1106 26-27 1.6072745685442982 1106 28-29 1.3725651436906396 1106 30-31 1.2135196227225116 1106 32-33 1.1105499790703988 1106 34-35 1.2944629852884404 1106 36-37 1.3299772120266908 1106 38-39 1.4751666595246888 1106 40-41 1.2486408172143157 1106 42-43 1.0667525081526392 1106 44-45 0.8373403727382147 1106 46-47 0.6707151333082706 1106 48-49 0.8931304114817848 1106 50-51 0.8444205335605091 1106 52-53 0.712523405111078 1106 54-55 0.634108039351478 1106 56-57 0.7170230265856397 1106 58-59 0.6353596947925233 1106 60-61 0.6686487415006148 1106 62-63 0.6438041109254229 1106 64-65 0.6110553665783698 1106 66-67 0.46478842300450296 1106 68-69 0.6438880095388875 1106 70-71 0.6814784868265775 1106 72-73 0.6955602942247943 1106 74-75 0.7277279323477757 1106 76-77 0.58217949918118 1106 78-79 0.614261480166693 1106 80-81 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2216 76-77 -1.8606569513356241 2216 78-79 -2.0179369827756837 2216 80-81 -1.9724489927811817 2216 82-83 -1.8371809103851504 2216 84-85 -1.8648002999590751 2216 86-87 -1.8942617359697458 2216 88-89 -1.9797146944458177 2216 90-91 -2.0304152059499323 2216 92-93 -2.011113727685146 2216 94-95 -2.0703280797798946 2216 96-97 -2.020790826663312 2216 98-99 -2.067365073548128 2216 100-101 -2.0922257074024166 2216 102-103 -1.8840740952329753 2216 104-105 -2.1512789080286616 2216 106-107 -2.071639085133281 2216 108-109 -2.101583750248757 2216 110-111 -2.0839848388594184 2216 112-113 -2.1259681855828454 2216 114-115 -2.3045534778590167 2216 116-117 -2.293270244123857 2216 118-119 -2.369793521182462 2216 120-121 -2.417985650055307 2216 122-123 -2.4632097171166265 2216 124-125 -2.511761103658113 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 191597.0 3 30778.0 4 19029.0 5 14969.0 6 13861.0 7 14679.0 8 15429.0 9 15992.0 10 16886.0 11 17954.0 12 19352.0 13 21246.0 14 23187.0 15 20536.0 16 16179.0 17 14337.0 18 12739.0 19 12500.0 20 12244.0 21 13045.0 22 15453.0 23 20446.0 24 26994.0 25 33984.0 26 41677.0 27 47098.0 28 54205.0 29 63748.0 30 72451.0 31 82581.0 32 99987.0 33 125630.0 34 160876.0 35 217440.0 36 330861.0 37 423895.0 38 139112.0 39 445.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.12204343140882 18.416073786085857 12.552570766590044 23.90931201591528 2 15.113422027788953 19.967735218545375 40.70117273181214 24.217670021853525 3 19.441606242274688 25.100386098824963 28.607056199637636 26.850951459262713 4 11.922099210050796 15.990679270595912 37.20589180724382 34.88132971210946 5 13.200047391276042 38.428218997836254 34.288356505322014 14.083377105565683 6 32.15806756534937 36.52360032635265 17.484610240648696 13.833721867649281 7 28.862905625711626 32.417427349653245 20.697787819583894 18.021879205051235 8 26.377019368308357 35.766480608646646 19.473547174723926 18.38295284832107 9 26.72064728221603 16.300356155621692 19.50599933612495 37.47299722603732 10-11 24.97347803973604 27.03717764295781 27.275066688983923 20.71427762832222 12-13 25.689132771013607 24.26133878024975 27.68357264671135 22.36595580202529 14-15 22.567035949639813 25.624523468066723 25.43121148444234 26.377229097851124 16-17 22.474125573495947 29.21357879873262 25.810972661666256 22.501322966105175 18-19 22.232853652111807 27.71222927337827 28.125406217546818 21.92951085696311 20-21 22.84204161269669 26.896911161863834 28.38742258799945 21.873624637440024 22-23 22.72863725816217 26.826232550429523 28.064640355141908 22.380489836266403 24-25 22.495742268558132 26.922630590648033 28.23993954263958 22.341687598154255 26-27 22.216044280609925 27.240193517393756 28.05650403808236 22.487258163913957 28-29 22.19792602032256 27.20209233491105 28.21528910099381 22.384692543772584 30-31 22.332078573402445 27.079425474800388 28.050383654927476 22.538112296869688 32-33 22.066809437253017 27.238452554455293 27.994740414083218 22.699997594208472 34-35 22.52983900542983 26.969997036215187 28.214988826167346 22.285175132187632 36-37 22.473682188800854 26.812507202396958 27.9601396607949 22.753670948007287 38-39 22.24281223398438 27.113110321067396 28.21644026301219 22.427637181936035 40-41 22.57354796652227 26.968288682340074 27.99885797846012 22.459305372677537 42-43 22.294488519450443 27.02456712665419 27.823396004258477 22.857548349636886 44-45 22.506591702249356 27.196652804261067 27.63723185100822 22.659523642481354 46-47 22.580428656335034 27.46020515018336 27.208679866252105 22.750686327229502 48-49 22.585345978904503 27.495018304130635 27.098342872105636 22.821292844859222 50-51 22.539748128429597 27.513378756685334 27.094387957259137 22.85248515762593 52-53 22.9042799813376 27.366114637938853 27.000224789785165 22.729380590938383 54-55 22.659792955672813 27.255168526286123 27.14546358123362 22.939574936807443 56-57 22.513315719877728 27.73053879262855 26.995002827196153 22.761142660297573 58-59 22.678586770302164 27.302461679966445 27.019835955385524 22.999115594345866 60-61 22.728589516005616 27.350949853101326 27.23204006301693 22.688420567876122 62-63 22.615542769144163 27.39483023855087 27.327981966738434 22.661645025566532 64-65 22.55261470726969 27.198689527685282 27.221293638519832 23.027402126525196 66-67 22.37403027122487 27.751082328092984 26.947058509597422 22.927828891084722 68-69 22.531653247819353 27.395051943272854 27.269389257104038 22.80390555180376 70-71 22.356770241080643 27.437586990075168 27.22376702781762 22.981875741026567 72-73 22.61233231956644 27.383654530240047 27.044063176081867 22.959949974111645 74-75 22.48494645979264 27.567298982401507 27.024225349379392 22.923529208426466 76-77 22.61959923340045 27.353816420432953 27.173184380129868 22.85339996603673 78-79 22.503010539972713 27.590999976348286 27.05376237141401 22.85222711226499 80-81 22.49516492229206 27.654157964672343 27.067135966205907 22.78354114682969 82-83 22.650615464325792 27.573602320552492 26.98600475908136 22.789777456040355 84-85 22.595052522375884 27.567374269332124 26.926339298348605 22.91123390994339 86-87 22.543849239601492 27.46052754737594 27.228992483853904 22.766630729168664 88-89 22.62607931218401 27.422566313530965 26.989426147407976 22.961928226877053 90-91 22.543419220112032 27.596256654216884 27.14985191283967 22.710472212831416 92-93 22.67033087217808 27.49864401619458 27.05904852087083 22.771976590756505 94-95 22.5754277770757 27.23663844306837 27.079469227852897 23.10846455200303 96-97 22.72227599229027 27.17823871093097 27.08052253588133 23.018962760897434 98-99 22.390771240461252 27.239262092218457 27.004039179291166 23.365927488029122 100-101 22.358579252427518 27.20924872786526 27.282392684795642 23.14977933491158 102-103 22.604553529482637 27.178318135764947 27.07115890454601 23.14596943020641 104-105 22.59552678712213 27.12954689717352 27.27056792499363 23.004358390710724 106-107 22.354973608409892 27.21846123617072 27.28783884503262 23.13872631038677 108-109 22.438063541118336 27.272438750848018 26.84529772921887 23.444199978814776 110-111 22.345076078490354 27.193261743604296 27.242040762117075 23.219621415788268 112-113 22.492300141261783 27.281899328137293 27.247513768374343 22.97828676222658 114-115 22.42108301777861 27.504101604982896 26.92690531579326 23.147910061445238 116-117 22.163767444455495 27.368216988447585 27.10819261735361 23.35982294974331 118-119 22.304442994361658 27.367792475364087 26.93464762583983 23.393116904434425 120-121 22.17027883866849 27.372732365051704 27.147436979452937 23.30955181682687 122-123 22.36539094420604 27.37288663236601 27.038451182208295 23.223271241219653 124-125 22.402282881683185 27.28763836067808 26.859547495905012 23.45053126173373 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 505.0 1 541.5 2 683.5 3 774.0 4 637.0 5 494.0 6 497.0 7 643.5 8 922.5 9 1062.0 10 1056.5 11 1077.0 12 1163.5 13 1344.0 14 1514.0 15 1720.5 16 1938.5 17 2160.0 18 2388.0 19 2660.0 20 3157.0 21 3841.5 22 4746.5 23 5789.5 24 7177.5 25 9356.0 26 12276.0 27 15709.5 28 19018.0 29 22602.0 30 27755.0 31 33764.5 32 39815.0 33 45152.0 34 50844.5 35 56669.0 36 62289.5 37 68166.5 38 71200.0 39 72386.0 40 73184.5 41 75208.5 42 77581.0 43 80396.5 44 85083.5 45 87881.5 46 87570.5 47 86847.5 48 89628.5 49 91767.5 50 90663.5 51 88939.0 52 86826.5 53 86262.5 54 84753.5 55 78450.0 56 71647.5 57 65872.0 58 61796.5 59 57315.5 60 48752.0 61 38941.5 62 30115.0 63 23046.0 64 17421.0 65 13006.0 66 9640.5 67 7423.5 68 5652.5 69 4206.0 70 3088.0 71 2265.0 72 1739.5 73 1334.0 74 1059.0 75 805.0 76 566.0 77 361.5 78 239.0 79 178.0 80 139.0 81 92.5 82 66.0 83 59.5 84 54.0 85 44.5 86 39.5 87 33.0 88 22.5 89 18.0 90 15.0 91 15.0 92 12.0 93 9.5 94 8.5 95 7.0 96 4.0 97 3.0 98 3.0 99 2.5 100 7.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01322055031450355 2 0.005538884994149805 3 0.00893498966209567 4 0.009096708931997854 5 0.015888918267889587 6 9.703156194131046E-4 7 0.008328542399962481 8 0.0 9 0.0026279381359104914 10-11 0.0 12-13 0.0038812624776524184 14-15 0.01926480802709768 16-17 0.03066601655520166 18-19 0.005963398077643039 20-21 0.008510476578602438 22-23 0.0017991268776617981 24-25 0.01107776998829961 26-27 0.018840294943604445 28-29 5.255876271820984E-4 30-31 0.001637407607759614 32-33 0.009036064205784537 34-35 0.009420147471802223 36-37 0.011603357615481709 38-39 2.0214908737773012E-5 40-41 0.024823907929985258 42-43 0.009662726376655498 44-45 0.01724331715332038 46-47 0.004528139557261154 48-49 0.025046271926100764 50-51 0.00396212211260351 52-53 0.0 54-55 0.01718267242710706 56-57 0.004993082458229933 58-59 3.840832660176872E-4 60-61 0.004790933370852204 62-63 0.026602819898909287 64-65 0.017000738248467102 66-67 0.0020214908737773013 68-69 0.021508662896990487 70-71 0.003214170489305909 72-73 0.012230019786352672 74-75 2.0214908737773012E-5 76-77 0.006549630431038456 78-79 0.0011522497980530617 80-81 0.0036588984815369148 82-83 0.0018395566951373442 84-85 0.0 86-87 0.005922968260167492 88-89 0.01758697060186252 90-91 0.022398118881452496 92-93 0.005073942093181026 94-95 0.024641973751345303 96-97 0.017930624050404662 98-99 9.501007106753316E-4 100-101 0.008773270392193487 102-103 0.0 104-105 0.0012937541592174728 106-107 6.064472621331904E-5 108-109 0.0 110-111 9.096708931997855E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 1.0107454368886506E-4 122-123 0.0 124-125 0.023550368679505558 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2473422.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.85988622163342 #Duplication Level Percentage of deduplicated Percentage of total 1 80.48590674629192 46.56905406785447 2 10.620904583742197 12.290486615722966 3 3.600787164021853 6.250234068558674 4 1.6605324226441205 3.8431286816608843 5 0.8922272375886238 2.5812083225360025 6 0.5767377469565672 2.002198825117691 7 0.3651724243645531 1.4790184447507757 8 0.28035866873457854 1.2977216539385064 9 0.2032499028344647 1.058401462730416 >10 1.1670728309515477 12.571879457961815 >50 0.09628581049210025 3.868950151892128 >100 0.04847667012239369 5.053047652480543 >500 0.002001817348210804 0.7439683065037851 >1k 2.859739068867196E-4 0.3907022882912247 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3787 0.1531077187798928 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2752 0.11126285769270267 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.0429817475546025E-5 2 4.0429817475546025E-5 0.0 0.0 0.0 4.0429817475546025E-5 3 4.0429817475546025E-5 0.0 0.0 0.0 4.0429817475546025E-5 4 4.0429817475546025E-5 0.0 0.0 0.0 4.0429817475546025E-5 5 4.0429817475546025E-5 4.0429817475546025E-5 0.0 4.0429817475546025E-5 4.0429817475546025E-5 6 4.0429817475546025E-5 4.0429817475546025E-5 0.0 4.0429817475546025E-5 8.085963495109205E-5 7 8.085963495109205E-5 4.0429817475546025E-5 0.0 4.0429817475546025E-5 8.085963495109205E-5 8 8.085963495109205E-5 4.0429817475546025E-5 0.0 4.0429817475546025E-5 8.085963495109205E-5 9 8.085963495109205E-5 4.0429817475546025E-5 0.0 4.0429817475546025E-5 8.085963495109205E-5 10-11 1.2128945242663807E-4 4.0429817475546025E-5 0.0 1.4150436116441107E-4 8.085963495109205E-5 12-13 1.2128945242663807E-4 4.0429817475546025E-5 0.0 1.617192699021841E-4 1.4150436116441107E-4 14-15 2.4257890485327615E-4 4.0429817475546025E-5 0.0 1.8193417863995713E-4 1.617192699021841E-4 16-17 2.627938135910491E-4 4.0429817475546025E-5 0.0 3.234385398043682E-4 1.617192699021841E-4 18-19 3.638683572799142E-4 4.0429817475546025E-5 0.0 4.4472799223100625E-4 1.617192699021841E-4 20-21 4.4472799223100625E-4 4.0429817475546025E-5 0.0 4.851578097065523E-4 2.0214908737773012E-4 22-23 4.4472799223100625E-4 4.0429817475546025E-5 0.0 7.277367145598285E-4 2.4257890485327615E-4 24-25 6.468770796087364E-4 4.0429817475546025E-5 0.0 0.0010107454368886507 2.4257890485327615E-4 26-27 6.468770796087364E-4 4.0429817475546025E-5 0.0 0.0011724647067908349 2.4257890485327615E-4 28-29 6.873068970842825E-4 4.0429817475546025E-5 0.0 0.0021427803262039392 2.4257890485327615E-4 30-31 7.277367145598284E-4 4.0429817475546025E-5 0.0 0.004123841382505694 2.4257890485327615E-4 32-33 7.277367145598284E-4 4.0429817475546025E-5 0.0 0.008914774753357899 2.4257890485327615E-4 34-35 7.883814407731475E-4 4.0429817475546025E-5 0.0 0.014433444838769932 2.4257890485327615E-4 36-37 8.894559844620125E-4 4.0429817475546025E-5 0.0 0.025167561378527402 2.4257890485327615E-4 38-39 0.0010511752543641967 4.0429817475546025E-5 0.0 0.04499838685028272 2.4257890485327615E-4 40-41 0.0010713901631019696 4.0429817475546025E-5 0.0 0.06854875552978829 2.4257890485327615E-4 42-43 0.0011522497980530617 4.0429817475546025E-5 0.0 0.09282686092385367 2.4257890485327615E-4 44-45 0.0012533243417419267 4.0429817475546025E-5 0.0 0.11890409319558086 2.627938135910491E-4 46-47 0.0013543988854307918 6.064472621331904E-5 0.0 0.15237998206533296 2.8300872232882215E-4 48-49 0.001374613794168565 8.085963495109205E-5 0.0 0.19016164649623074 2.8300872232882215E-4 50-51 0.001374613794168565 8.085963495109205E-5 0.0 0.23230973121448745 2.8300872232882215E-4 52-53 0.001415043611644111 1.0107454368886506E-4 0.0 0.27298212759488677 3.234385398043682E-4 54-55 0.001415043611644111 1.8193417863995713E-4 0.0 0.3169293391908053 3.234385398043682E-4 56-57 0.0014554734291196568 2.0214908737773012E-4 0.0 0.36975089572260617 3.436534485421412E-4 58-59 0.0014554734291196568 2.0214908737773012E-4 0.0 0.4314265822815516 3.638683572799142E-4 60-61 0.0014554734291196568 2.0214908737773012E-4 0.0 0.5006424297996864 3.638683572799142E-4 62-63 0.0014554734291196568 2.0214908737773012E-4 0.0 0.5700402114964611 4.0429817475546025E-4 64-65 0.0014756883378574297 2.0214908737773012E-4 0.0 0.6384272477563473 4.4472799223100625E-4 66-67 0.001536333064070749 2.0214908737773012E-4 0.0 0.7221169699307275 4.4472799223100625E-4 68-69 0.001576762881546295 2.0214908737773012E-4 0.0 0.8245054826875479 4.4472799223100625E-4 70-71 0.001657622516497387 2.2236399611550312E-4 0.0 0.9402560501200361 4.4472799223100625E-4 72-73 0.001698052333972933 2.4257890485327615E-4 0.0 1.058270687331155 4.649429009687793E-4 74-75 0.001698052333972933 2.4257890485327615E-4 0.0 1.1738393205850033 4.851578097065523E-4 76-77 0.001758697060186252 2.4257890485327615E-4 0.0 1.298727026766965 4.851578097065523E-4 78-79 0.001778911968924025 2.4257890485327615E-4 0.0 1.4492472372284229 4.851578097065523E-4 80-81 0.001859771603875117 2.4257890485327615E-4 0.0 1.6229539480121065 4.851578097065523E-4 82-83 0.0019002014213506631 2.4257890485327615E-4 0.0 1.8108313098209687 5.255876271820984E-4 84-85 0.0019406312388262092 2.4257890485327615E-4 0.0 1.9920377517463659 5.255876271820984E-4 86-87 0.001981061056301755 2.4257890485327615E-4 0.0 2.183937880394045 5.660174446576443E-4 88-89 0.001981061056301755 2.4257890485327615E-4 0.0 2.4074339113988636 6.064472621331903E-4 90-91 0.0021427803262039392 2.627938135910491E-4 0.0 2.6548643943492056 6.064472621331903E-4 92-93 0.002264069778630577 3.032236310665952E-4 0.0 2.918224225384912 6.873068970842825E-4 94-95 0.002264069778630577 3.234385398043682E-4 0.0 3.1915095766108657 6.873068970842825E-4 96-97 0.002264069778630577 3.638683572799142E-4 0.0 3.4669377081630226 7.277367145598285E-4 98-99 0.0023651443223194424 3.638683572799142E-4 0.0 3.76070076194034 7.681665320353744E-4 100-101 0.0025268635922216266 3.638683572799142E-4 0.0 4.104859583200925 7.681665320353744E-4 102-103 0.0026683679533860377 3.638683572799142E-4 0.0 4.484475354387564 7.883814407731475E-4 104-105 0.003072666128141498 3.638683572799142E-4 0.0 4.856914832972295 8.490261669864666E-4 106-107 0.003234385398043682 3.638683572799142E-4 0.0 5.229536245735664 8.490261669864666E-4 108-109 0.003295030124257001 3.638683572799142E-4 0.0 5.6266581278892165 8.490261669864666E-4 110-111 0.003335459941732547 3.638683572799142E-4 0.0 6.072720304096915 8.894559844620125E-4 112-113 0.00335567485047032 3.638683572799142E-4 0.0 6.553956421508339 8.894559844620125E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4250 0.0 53.057266 1 GTATCAA 6715 0.0 49.88351 1 ATCAACG 8010 0.0 41.223637 3 GTACATG 11155 0.0 40.002422 1 CAACGCA 8285 0.0 39.7117 5 TCAACGC 8340 0.0 39.307133 4 AACGCAG 8450 0.0 39.00273 6 TATCAAC 8810 0.0 37.61535 2 TACATGG 11665 0.0 37.130642 2 ACATGGG 11930 0.0 35.30845 3 ACGCAGA 9930 0.0 32.770267 7 CGCAGAG 9995 0.0 31.72773 8 CATGGGA 6775 0.0 29.50637 4 CATGGGG 5775 0.0 28.125256 4 GCAGAGT 10885 0.0 27.767622 9 ATGGGAG 3375 0.0 25.208563 5 GAGTACT 5465 0.0 24.935299 12-13 CAGAGTA 10630 0.0 24.489939 10-11 GAGTACA 8235 0.0 24.275618 1 AGAGTAC 9875 0.0 23.43988 10-11 >>END_MODULE