FastQCFastQC Report
Thu 26 May 2016
SRR1364588_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364588_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2473422
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57630.23299703811157174No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50160.20279596445733888No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32590.13176077515280452No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50050.060.7432061
GTATCAA81350.050.7817081
ATCAACG103000.039.611793
TCAACGC105050.038.6119844
CAACGCA105500.038.2933045
TATCAAC108650.037.5519032
AACGCAG107950.037.313986
GTACATG108550.034.762061
TACATGG110700.033.090142
ACATGGG110350.032.0076263
ACGCAGA130150.030.6749527
CGCAGAG132100.029.996948
TGGTATC23850.027.2213212
GCAGAGT141400.026.7190539
CATGGGG52900.026.4596024
GAGTACT85700.026.31154612-13
GTGGTAT27300.025.5478781
GTACTTT92150.024.27618814-15
CATGGGA62550.024.186754
CAGAGTA138150.024.11710210-11
Produced by FastQC (version 0.11.5)