##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364588_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2473422 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.94221608767125 31.0 30.0 34.0 26.0 34.0 2 29.864234247128067 31.0 30.0 34.0 26.0 34.0 3 30.127791375673056 31.0 30.0 34.0 26.0 34.0 4 33.64406154711974 35.0 35.0 37.0 28.0 37.0 5 33.065494687117685 35.0 33.0 37.0 26.0 37.0 6 33.07385153038988 35.0 33.0 37.0 26.0 37.0 7 32.84736733157545 35.0 33.0 37.0 25.0 37.0 8 32.77518191396373 35.0 33.0 37.0 25.0 37.0 9 34.00644046992385 38.0 34.0 39.0 25.0 39.0 10-11 33.9355785223872 38.0 33.5 39.0 25.0 39.0 12-13 33.8172064855896 38.0 33.0 39.0 23.0 39.0 14-15 34.77636994415025 39.0 34.0 41.0 23.5 41.0 16-17 34.77922166132589 39.0 34.0 41.0 24.0 41.0 18-19 34.788414997521656 39.0 34.0 41.0 23.0 41.0 20-21 34.80324809110617 39.0 34.0 41.0 23.0 41.0 22-23 34.76121826360403 39.0 34.0 41.0 23.0 41.0 24-25 34.72008719094437 39.0 34.0 41.0 23.5 41.0 26-27 34.60421189752497 39.0 34.0 41.0 23.0 41.0 28-29 34.45995184000142 39.0 33.0 41.0 22.5 41.0 30-31 34.30850295663255 38.0 33.0 41.0 21.5 41.0 32-33 34.11790426380941 38.0 33.0 40.0 20.0 41.0 34-35 33.938987564596744 38.0 33.0 40.0 18.5 41.0 36-37 33.744663264093234 38.0 33.0 40.0 17.0 41.0 38-39 33.58004093114721 38.0 33.0 40.0 16.0 41.0 40-41 33.613487710548384 38.0 33.0 40.0 16.0 41.0 42-43 33.484694079700105 38.0 33.0 40.0 15.0 41.0 44-45 33.32905080491723 38.0 32.0 40.0 12.5 41.0 46-47 33.09762406091642 38.0 32.0 40.0 8.5 41.0 48-49 32.83127080619482 38.0 32.0 40.0 7.0 41.0 50-51 32.66238049956699 38.0 31.0 40.0 2.0 41.0 52-53 32.480268025431975 38.0 31.0 40.0 2.0 41.0 54-55 32.28982397666068 37.5 31.0 40.0 2.0 41.0 56-57 32.07292467682426 37.0 31.0 40.0 2.0 41.0 58-59 31.867533926681332 37.0 31.0 40.0 2.0 41.0 60-61 31.628953530776393 36.5 30.0 40.0 2.0 41.0 62-63 31.45573784012595 36.0 30.0 40.0 2.0 41.0 64-65 31.31374286312647 36.0 30.0 39.0 2.0 41.0 66-67 31.016919272166255 35.0 30.0 39.0 2.0 41.0 68-69 30.72959527326918 35.0 30.0 39.0 2.0 41.0 70-71 30.418603052774657 35.0 29.0 38.5 2.0 40.0 72-73 30.13289523583117 35.0 29.0 37.0 2.0 40.0 74-75 29.7828585255569 35.0 29.0 37.0 2.0 39.0 76-77 29.472924151236626 35.0 29.0 36.5 2.0 39.0 78-79 29.183763425731637 34.0 29.0 36.0 2.0 39.0 80-81 28.905621846979606 34.0 29.0 35.5 2.0 37.0 82-83 28.61950366738874 34.0 29.0 35.0 2.0 37.0 84-85 28.394777357038144 34.0 28.5 35.0 2.0 36.5 86-87 28.184227964334433 34.0 28.0 35.0 2.0 36.0 88-89 28.010031446312034 34.0 28.0 35.0 2.0 36.0 90-91 27.866532480102464 34.0 27.5 35.0 2.0 36.0 92-93 27.746650793920324 34.0 27.0 35.0 2.0 35.0 94-95 27.63503660111376 34.0 27.0 35.0 2.0 35.0 96-97 27.522305332450347 34.0 27.0 35.0 2.0 35.0 98-99 27.440669242854636 34.0 27.0 35.0 2.0 35.0 100-101 27.33854413035867 34.0 27.0 35.0 2.0 35.0 102-103 26.07096706506209 31.5 24.5 34.0 2.0 35.0 104-105 26.999860517129708 33.5 25.5 35.0 2.0 35.0 106-107 27.1587001732822 34.0 26.0 35.0 2.0 35.0 108-109 27.168044514846233 34.0 27.0 35.0 2.0 35.0 110-111 27.0861749430546 34.0 26.0 35.0 2.0 35.0 112-113 27.00105137740345 34.0 25.5 35.0 2.0 35.0 114-115 26.897618562461236 34.0 25.0 35.0 2.0 35.0 116-117 26.76964606120589 34.0 25.0 35.0 2.0 35.0 118-119 26.636328131633014 34.0 25.0 35.0 2.0 35.0 120-121 26.435709919294 34.0 25.0 35.0 2.0 35.0 122-123 26.141283614360994 34.0 24.5 35.0 2.0 35.0 124-125 24.3357249591861 31.5 12.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7167278201442002 1101 2 0.8026756407093139 1101 3 0.6805436375413763 1101 4 0.6981409445791797 1101 5 0.750188601102117 1101 6 0.7272432085647225 1101 7 0.8586061269090592 1101 8 0.9037420579781852 1101 9 1.1329549950813842 1101 10-11 1.0770037604829525 1101 12-13 1.0125977666122736 1101 14-15 1.1457142628877008 1101 16-17 1.1582179053837578 1101 18-19 1.2978610436847902 1101 20-21 1.2923296362401828 1101 22-23 1.2531947377031258 1101 24-25 1.248818400304934 1101 26-27 1.0904402714791885 1101 28-29 1.163279513680962 1101 30-31 1.2504319264945423 1101 32-33 1.335740109876781 1101 34-35 1.4136572098235476 1101 36-37 1.457979980715706 1101 38-39 1.3105495271856284 1101 40-41 1.1849072577843458 1101 42-43 1.1500109158341516 1101 44-45 1.170133161367744 1101 46-47 1.17090684286174 1101 48-49 1.3126927420223709 1101 50-51 1.32873177349353 1101 52-53 1.2661597960574653 1101 54-55 1.246421160092467 1101 56-57 1.3068581055256772 1101 58-59 1.2253584850033334 1101 60-61 1.2014406598813743 1101 62-63 1.2443382138261079 1101 64-65 1.2280441892058533 1101 66-67 1.3038863427849172 1101 68-69 1.3414724845143162 1101 70-71 1.198000794347518 1101 72-73 1.209442073572152 1101 74-75 1.283006573238815 1101 76-77 1.221853383317871 1101 78-79 1.1920506113265148 1101 80-81 1.0115501091560581 1101 82-83 1.0796619869383726 1101 84-85 1.171308265982745 1101 86-87 1.1963377943289082 1101 88-89 1.1793779985809358 1101 90-91 1.2210198494488687 1101 92-93 1.167784320673153 1101 94-95 1.1659659159206832 1101 96-97 1.1597056097443463 1101 98-99 1.236600592589273 1101 100-101 1.1627856247559016 1101 102-103 1.1160964353838523 1101 104-105 1.1629449513221246 1101 106-107 1.0906998404904655 1101 108-109 1.1715848927951136 1101 110-111 1.0892310989934764 1101 112-113 1.1419094061095052 1101 114-115 1.136431111862695 1101 116-117 1.1144196966982172 1101 118-119 1.22923841262946 1101 120-121 1.12513417649199 1101 122-123 1.150600147351728 1101 124-125 1.4570548166973651 1102 1 0.24813627745869837 1102 2 -0.5022776610225108 1102 3 0.1787292103578224 1102 4 0.17524987591629326 1102 5 0.43099772486426247 1102 6 0.28796034377850077 1102 7 0.3018820456212197 1102 8 0.3050749532708181 1102 9 0.7515460684375626 1102 10-11 0.7876519042854611 1102 12-13 0.7957610462856266 1102 14-15 0.9591558548527814 1102 16-17 0.9524582958657604 1102 18-19 1.01742926070267 1102 20-21 0.949223614744902 1102 22-23 1.0894898519848084 1102 24-25 1.0295308422254266 1102 26-27 1.0369264027975333 1102 28-29 0.7398796920261077 1102 30-31 1.0481453540066283 1102 32-33 1.122364224017673 1102 34-35 1.1701438104712238 1102 36-37 0.8091199816543622 1102 38-39 0.20442196347416797 1102 40-41 -0.10707378131078826 1102 42-43 0.11382562461530199 1102 44-45 -0.04001937066398398 1102 46-47 0.1675595860916701 1102 48-49 -0.07829707353120341 1102 50-51 -0.13740705412180176 1102 52-53 -0.2827828049656773 1102 54-55 -0.18806797493358118 1102 56-57 -0.18589920427861273 1102 58-59 -0.23738427559177566 1102 60-61 -0.46959421128772405 1102 62-63 -0.4421178804152177 1102 64-65 -0.3856405153513407 1102 66-67 -0.42017711054027984 1102 68-69 -0.1948329550125969 1102 70-71 -0.7714055040520655 1102 72-73 -0.6187101768619776 1102 74-75 -0.44460031651572507 1102 76-77 -0.8032256986739625 1102 78-79 -0.5994488958776927 1102 80-81 -0.6990952186704824 1102 82-83 -0.9987648211528644 1102 84-85 -0.8003254694093798 1102 86-87 -0.5142803123965791 1102 88-89 -0.61329389328559 1102 90-91 -0.6412976987747214 1102 92-93 -0.49476507623866084 1102 94-95 -0.31455785560979876 1102 96-97 -0.5903061234888654 1102 98-99 -0.7048186592997787 1102 100-101 -0.5864629789893421 1102 102-103 -0.5841945487997506 1102 104-105 -0.40677955236211005 1102 106-107 -0.37483730692587613 1102 108-109 -0.4043206781440176 1102 110-111 -0.43572595737297704 1102 112-113 -0.24274352485214834 1102 114-115 -0.15998790536168883 1102 116-117 -0.25441120394476613 1102 118-119 -0.04151673825991864 1102 120-121 0.08589636732129691 1102 122-123 0.0679756577473718 1102 124-125 0.22810564506362851 1103 1 0.3701563274983073 1103 2 -0.15479090708098653 1103 3 0.27326045471791716 1103 4 0.2784601239676192 1103 5 0.3111109504053573 1103 6 0.349456022780295 1103 7 0.4210353617427458 1103 8 0.4873183767856304 1103 9 0.6280095560501948 1103 10-11 0.6849073247682682 1103 12-13 0.6065515543377415 1103 14-15 0.7577985921752273 1103 16-17 0.6266431764565041 1103 18-19 0.6931994128542414 1103 20-21 0.763832711163289 1103 22-23 0.7978949486539619 1103 24-25 0.7110371218735168 1103 26-27 0.7893396076312698 1103 28-29 0.8814343774927735 1103 30-31 0.8673518253402364 1103 32-33 0.8999327625153057 1103 34-35 0.8304793456233881 1103 36-37 1.0078094150137176 1103 38-39 0.9926560819772376 1103 40-41 0.8480538348926245 1103 42-43 0.8190013236785347 1103 44-45 0.7890428900853124 1103 46-47 0.8689607711603315 1103 48-49 0.7954860083692381 1103 50-51 0.8287921962339269 1103 52-53 0.8824349547264063 1103 54-55 0.9199661371103076 1103 56-57 0.9566530437993066 1103 58-59 0.9893258195290784 1103 60-61 1.0406242418158094 1103 62-63 0.9065974846326661 1103 64-65 0.8946119624072466 1103 66-67 0.8644076119906536 1103 68-69 0.8809982553418401 1103 70-71 0.8930427019120302 1103 72-73 0.8429033630889684 1103 74-75 0.8803975652547713 1103 76-77 0.8252275758428809 1103 78-79 0.7658749842628154 1103 80-81 0.7816152905974896 1103 82-83 0.8986250641587787 1103 84-85 0.9508989812282742 1103 86-87 0.9270772162470138 1103 88-89 0.8738445591340387 1103 90-91 0.842532934053466 1103 92-93 0.8276212850230102 1103 94-95 0.8686691862969518 1103 96-97 0.7869521086348925 1103 98-99 0.858255490131338 1103 100-101 0.8174540928005882 1103 102-103 0.5860524766551016 1103 104-105 0.6312458397953122 1103 106-107 0.7309515958312431 1103 108-109 0.8219118939565284 1103 110-111 0.6602321947709946 1103 112-113 0.7713345513775174 1103 114-115 0.851684736042003 1103 116-117 0.9316267950027672 1103 118-119 0.7695249557157631 1103 120-121 0.8382155721588376 1103 122-123 0.6897437999556146 1103 124-125 1.0858228965706118 1104 1 0.5111401682765937 1104 2 0.7023487868214673 1104 3 0.45744684761100274 1104 4 0.1751972508289299 1104 5 0.574129141817167 1104 6 0.7267985552730494 1104 7 0.7776082755644467 1104 8 0.648090879087384 1104 9 0.7881039840654935 1104 10-11 0.8633583998209247 1104 12-13 0.8240813790794945 1104 14-15 0.9418466114505364 1104 16-17 0.8591426163555269 1104 18-19 0.7653547932139873 1104 20-21 0.8207979570247446 1104 22-23 0.8251895077539473 1104 24-25 0.8700694271039353 1104 26-27 0.8389612145918264 1104 28-29 0.9803196422415752 1104 30-31 0.9198296931842833 1104 32-33 0.9389361181844293 1104 34-35 1.0819861370382995 1104 36-37 1.1111511306229716 1104 38-39 1.1588399292603881 1104 40-41 1.072872958213722 1104 42-43 1.1659545486041907 1104 44-45 1.0860658535719594 1104 46-47 1.2037367547274158 1104 48-49 1.1447146661988654 1104 50-51 0.9737363828794585 1104 52-53 1.0862417247536627 1104 54-55 0.6961264463586474 1104 56-57 0.8558066633438699 1104 58-59 0.9794715996927437 1104 60-61 1.122174036498695 1104 62-63 1.1048697818423499 1104 64-65 1.2014188336374545 1104 66-67 1.2166806709086337 1104 68-69 0.9648939402193726 1104 70-71 0.566543762074641 1104 72-73 0.5868587010799615 1104 74-75 1.0076802036055206 1104 76-77 1.1216131930596376 1104 78-79 1.1148786579153906 1104 80-81 1.091937548669076 1104 82-83 1.037938112471501 1104 84-85 1.03198711912448 1104 86-87 1.018992908236342 1104 88-89 0.9989862904537112 1104 90-91 1.061139836965861 1104 92-93 1.0311193279728421 1104 94-95 1.033045609871806 1104 96-97 1.052849139206753 1104 98-99 1.0411636694350896 1104 100-101 0.9744007751079771 1104 102-103 0.825328013085187 1104 104-105 0.9413700479040052 1104 106-107 0.9846977384669984 1104 108-109 1.045518905041373 1104 110-111 0.950793124827559 1104 112-113 0.9341386629760571 1104 114-115 0.8561761070022165 1104 116-117 0.9361401634478632 1104 118-119 0.9036650014327776 1104 120-121 0.791862624474085 1104 122-123 0.7987677998650113 1104 124-125 1.2343893720261363 1105 1 0.24457417584349983 1105 2 0.012198894869975163 1105 3 0.23075718238579412 1105 4 0.20514611537005578 1105 5 -0.13751600329548808 1105 6 0.27880796223674054 1105 7 0.509224084113697 1105 8 0.4723287024714651 1105 9 0.6326674249571482 1105 10-11 0.5292406989715985 1105 12-13 0.5507058654782284 1105 14-15 0.7718245430966917 1105 16-17 0.6746304599848187 1105 18-19 0.7065804045440771 1105 20-21 0.6850719551950064 1105 22-23 0.7898962302672956 1105 24-25 0.7236245361421325 1105 26-27 0.7857120124681032 1105 28-29 0.6978725546207372 1105 30-31 0.7832122929673702 1105 32-33 0.9721453573386256 1105 34-35 1.0269748850014153 1105 36-37 1.0667485477785021 1105 38-39 1.0592553896350339 1105 40-41 0.7778997112733279 1105 42-43 0.7076536255229513 1105 44-45 0.7967036113687271 1105 46-47 0.6641773635944048 1105 48-49 0.6606129250248074 1105 50-51 0.510422638309656 1105 52-53 0.6598308056125362 1105 54-55 0.5991523563575498 1105 56-57 0.6017118277571996 1105 58-59 0.21882093512670764 1105 60-61 0.3810273205727448 1105 62-63 0.5959612086373234 1105 64-65 0.32856745956460287 1105 66-67 0.45881993960721346 1105 68-69 0.5398441083370322 1105 70-71 0.634324223447571 1105 72-73 0.6530347743519975 1105 74-75 0.6111960405195696 1105 76-77 0.5887804073144416 1105 78-79 0.43220157754269195 1105 80-81 0.5184710498692375 1105 82-83 0.5487591592914711 1105 84-85 0.5869163153545287 1105 86-87 0.7737201826835616 1105 88-89 0.6211286163581597 1105 90-91 0.3644097501185648 1105 92-93 0.5662494254838073 1105 94-95 0.5846706445036567 1105 96-97 0.6447760804428988 1105 98-99 0.5470954442957421 1105 100-101 0.5866298279087729 1105 102-103 0.2395292789948762 1105 104-105 0.4120413317685063 1105 106-107 0.3596784428111164 1105 108-109 0.3380235263240827 1105 110-111 0.3444700118495412 1105 112-113 0.3428793478503671 1105 114-115 0.3409985418194488 1105 116-117 0.12084015236854029 1105 118-119 0.2838156874264861 1105 120-121 0.3457672339178295 1105 122-123 0.3287471576339165 1105 124-125 0.8019926430214213 1106 1 -0.1412392460756422 1106 2 -0.5672518254852328 1106 3 -0.005557512340743642 1106 4 -0.16688077943104673 1106 5 0.0694688634161551 1106 6 0.3140635541616561 1106 7 0.40372432178381956 1106 8 0.4374839171198843 1106 9 0.45958510880500114 1106 10-11 0.3615989531988575 1106 12-13 0.49254049832752145 1106 14-15 0.4470162582448083 1106 16-17 0.316493533844465 1106 18-19 0.4111756489039635 1106 20-21 0.35199578201235937 1106 22-23 0.3092782735228923 1106 24-25 0.4154051563366181 1106 26-27 0.47148446922638954 1106 28-29 0.5782879593980326 1106 30-31 0.45101201628109067 1106 32-33 0.3507097143281612 1106 34-35 0.4088378217089712 1106 36-37 0.461278778443031 1106 38-39 0.37325197599193416 1106 40-41 0.4249012961017087 1106 42-43 0.31511881325707947 1106 44-45 0.1906034116680786 1106 46-47 0.11673068417259458 1106 48-49 0.2571110714866336 1106 50-51 0.34244292093678297 1106 52-53 0.16817918055889436 1106 54-55 0.20615559422942198 1106 56-57 -0.3154228703837063 1106 58-59 0.2328810445524141 1106 60-61 0.3170001095999986 1106 62-63 0.15443098122112886 1106 64-65 0.3076866689045161 1106 66-67 0.19665391845763835 1106 68-69 0.3022209466711807 1106 70-71 0.435854046626055 1106 72-73 0.3705072275371215 1106 74-75 0.28285951840036816 1106 76-77 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-1.3536060171086106 2216 84-85 -1.3178352661142227 2216 86-87 -1.505916849234353 2216 88-89 -1.412040348069194 2216 90-91 -1.3869674469831708 2216 92-93 -1.395659228526 2216 94-95 -1.309095516091734 2216 96-97 -1.402846771695149 2216 98-99 -1.4156420155101301 2216 100-101 -1.4193097606906129 2216 102-103 -1.3007153654444075 2216 104-105 -1.2760696430763474 2216 106-107 -1.3124882627811658 2216 108-109 -1.3453274113488938 2216 110-111 -1.3257772682911586 2216 112-113 -1.310244877988179 2216 114-115 -1.357475618886557 2216 116-117 -1.3582255320315362 2216 118-119 -1.376867579369275 2216 120-121 -1.4463662926193805 2216 122-123 -1.4210814770682738 2216 124-125 -1.7243886717650554 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 86047.0 3 27901.0 4 13246.0 5 10205.0 6 10834.0 7 12681.0 8 14442.0 9 15536.0 10 16865.0 11 18420.0 12 20125.0 13 22813.0 14 25840.0 15 23061.0 16 15991.0 17 13950.0 18 13321.0 19 12647.0 20 12547.0 21 12477.0 22 14320.0 23 17722.0 24 23586.0 25 30626.0 26 38561.0 27 43174.0 28 49481.0 29 58676.0 30 67709.0 31 80328.0 32 97009.0 33 121491.0 34 155899.0 35 212678.0 36 344467.0 37 526089.0 38 191959.0 39 698.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.74333561313978 19.281860969578748 13.487173053497209 24.487630363784262 2 15.455644411381812 20.557082905450752 39.32085937689515 24.666413306272286 3 19.34336316245267 24.95708374874971 29.11662466008631 26.582928428711316 4 12.62765885547247 16.80023926107503 36.15792526005275 34.41417662339975 5 12.965963753860038 38.81808280188338 34.15603968914322 14.059913755113362 6 32.357276679838705 37.05748554027578 16.848479555854198 13.736758224031323 7 28.51178171294434 32.665956972822876 20.464168883954546 18.35809243027824 8 26.314559476513207 36.127147063271096 19.077517869113745 18.480775591101953 9 26.409765012210308 15.862605728332769 19.48300260378762 38.2446266556693 10-11 25.091225327316536 27.0197785768101 27.002373445389228 20.886622650484142 12-13 25.777889903138245 24.15172178463683 27.817998707863033 22.25238960436189 14-15 22.550876407792366 25.47126539947413 25.6383619364358 26.339496256297707 16-17 22.28082999550014 28.95386169140511 26.225463540026304 22.539844773068445 18-19 22.134427063307527 27.110833756844404 28.82899492231453 21.925744257533545 20-21 22.68089628567807 26.355737914692227 29.075026921601992 21.888338878027707 22-23 22.56056588806924 26.245056444068176 28.85795468787777 22.336422979984818 24-25 22.39031592667972 26.48640223948845 28.84473413756326 22.278547696268568 26-27 22.119476579411035 26.775192425716277 28.770221983955828 22.33510901091686 28-29 22.061480006242366 26.67237939987596 29.02222103628091 22.243919557600766 30-31 22.206137893169867 26.48456268279331 28.934003174549268 22.375296249487555 32-33 21.94035227308563 26.670357909002185 28.894119159609644 22.495170658302545 34-35 22.45866253312213 26.526933131507686 28.781279539035392 22.233124796334796 36-37 22.348956223402233 26.380718696607374 28.632841464173925 22.637483615816468 38-39 22.15992661179532 26.740341923052352 28.809438906907108 22.290292558245216 40-41 22.396441044027263 26.461052743931283 28.801676381951808 22.340829830089646 42-43 22.238663681328944 26.574478596858924 28.51278512118029 22.67407260063184 44-45 22.41598886077669 26.718752400520412 28.335601446093712 22.529657292609187 46-47 22.446533587879465 27.017185098216157 27.850868149470653 22.685413164433726 48-49 22.581306384434196 27.126588184304985 27.60036500039217 22.691740430868652 50-51 22.518754898328044 27.204816164497707 27.535875925666502 22.740553011507746 52-53 22.869995496118335 27.070431167831448 27.40864680592313 22.650926530127087 54-55 22.626607994915545 26.92739047360297 27.56268441050496 22.883317120976525 56-57 22.48129873942122 27.384544658015898 27.378904258360226 22.75525234420266 58-59 22.63221965850288 27.068981480171246 27.440413066532223 22.85838579479365 60-61 22.641486167746546 27.108414981349725 27.663112077114217 22.586986773789512 62-63 22.495655816112254 27.1102747529536 27.746822014197335 22.647247416736814 64-65 22.425874692176357 26.886108385457664 27.690199736154053 22.99781718621193 66-67 22.2566751650143 27.44505385655986 27.395729479239694 22.902541499186146 68-69 22.406916896291232 27.138763045441305 27.63509980001387 22.819220258253598 70-71 22.198961600567955 27.12523378541955 27.665396361801587 23.010408252210905 72-73 22.48443654176279 27.161014173885413 27.335104967935113 23.01944431641669 74-75 22.370431135785342 27.356350233313815 27.258609707221137 23.01460892367971 76-77 22.482758704337552 27.11591471249144 27.439110673390953 22.962215909780053 78-79 22.379298801417633 27.374483610156293 27.35374311379134 22.892474474634735 80-81 22.377046181226508 27.361079412966305 27.33734707652587 22.924527329281315 82-83 22.508350778799574 27.300860912533327 27.247008395655897 22.943779913011205 84-85 22.44590692570859 27.298738347115858 27.158770319015517 23.09658440816003 86-87 22.397815657821432 27.165380594172774 27.442911076233656 22.993892671772144 88-89 22.478157508520695 27.175576839883725 27.20139161232155 23.144874039274033 90-91 22.37341626297494 27.35406655233114 27.40393673218723 22.86858045250669 92-93 22.443840366204295 27.193689105183672 27.32618021499786 23.03629031361418 94-95 22.357648634159478 26.965030633672697 27.318912826036158 23.358407906131667 96-97 22.488970276809006 26.890569255623326 27.350842743025396 23.26961772454228 98-99 22.17475788606322 26.97383556265892 27.216970394841912 23.63443615643595 100-101 22.045299438335807 26.94532628436413 27.521788139554065 23.487586137746 102-103 22.374265289141924 26.87911727153717 27.257560578017014 23.489056861303894 104-105 22.370384026664272 26.825830772104396 27.415398585441547 23.388386615789784 106-107 22.164013444778828 26.956935185565158 27.394333212109036 23.48471815754698 108-109 22.211272479989262 26.972894233171697 26.997657496375467 23.818175790463577 110-111 22.087539133576005 26.893355534716214 27.3708523766389 23.648252955068884 112-113 22.22194595180281 26.98176857810758 27.32176312816818 23.474522341921435 114-115 22.06606879052584 27.21850537433564 26.98093976684933 23.734486068289197 116-117 21.87762541127232 27.024523110087966 27.18761699378432 23.910234484855394 118-119 21.98310680506602 27.088361791881855 26.958541647967877 23.96998975508425 120-121 21.830193256246464 27.067073663782647 27.129700008086033 23.973033071884856 122-123 21.969941398179436 27.05654222573709 26.99160878857495 23.981907587508523 124-125 21.85434416229482 27.003445547999945 26.973380460930564 24.168829828774676 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 195.0 1 169.5 2 235.5 3 362.0 4 303.0 5 241.0 6 436.5 7 1033.5 8 1682.0 9 1910.5 10 1607.0 11 1220.5 12 1199.0 13 1329.5 14 1528.0 15 1771.0 16 2045.0 17 2344.0 18 2718.5 19 3157.0 20 3652.5 21 4290.0 22 5220.5 23 6329.5 24 7690.5 25 9763.5 26 12742.5 27 16190.0 28 19328.0 29 22991.0 30 28138.5 31 33606.5 32 39397.0 33 44887.0 34 50511.0 35 56236.0 36 61947.0 37 67313.0 38 70169.5 39 71963.5 40 73057.0 41 74949.5 42 77358.0 43 80703.0 44 84956.0 45 87580.0 46 87290.5 47 86413.5 48 89728.0 49 92410.5 50 91721.5 51 89857.0 52 87408.0 53 87263.0 54 86038.0 55 79347.0 56 71931.5 57 65821.5 58 62249.0 59 57949.0 60 48851.0 61 38470.5 62 29275.5 63 22171.5 64 16644.0 65 12264.0 66 8909.0 67 6719.0 68 5078.5 69 3613.0 70 2523.5 71 1913.0 72 1472.5 73 1084.0 74 878.5 75 678.0 76 431.0 77 223.0 78 151.0 79 102.0 80 62.0 81 37.5 82 25.5 83 16.5 84 13.5 85 10.0 86 3.5 87 2.0 88 3.0 89 2.0 90 0.5 91 1.5 92 1.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09913391245003884 2 8.085963495109205E-5 3 0.0 4 0.10030637715682969 5 0.0 6 0.0 7 0.0022236399611550313 8 0.006468770796087364 9 0.004123841382505694 10-11 5.458025359198714E-4 12-13 0.0 14-15 6.064472621331904E-5 16-17 4.4472799223100625E-4 18-19 0.002061920691252847 20-21 0.00147568833785743 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 6.064472621331904E-5 52-53 0.0 54-55 0.0 56-57 0.007802954772780382 58-59 7.681665320353744E-4 60-61 0.0 62-63 0.0 64-65 3.6386835727991425E-4 66-67 0.0 68-69 2.0214908737773012E-5 70-71 0.0 72-73 0.0 74-75 0.00147568833785743 76-77 0.0 78-79 0.0 80-81 1.4150436116441107E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0012937541592174728 90-91 0.0 92-93 0.0019608461475639823 94-95 0.0 96-97 0.008833915118406806 98-99 0.0041642711999812405 100-101 0.0023449294135816693 102-103 0.0 104-105 0.0 106-107 0.0018395566951373442 108-109 0.0 110-111 1.0107454368886506E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 8.894559844620125E-4 122-123 0.004851578097065523 124-125 0.08558992359573094 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2473422.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.57642767400345 #Duplication Level Percentage of deduplicated Percentage of total 1 77.42449114609944 40.70703158939417 2 11.907295833712151 12.520861563882587 3 4.253387654473025 6.708837851547006 4 1.9950597293354997 4.195724542588992 5 1.0993102246555375 2.8898902258947174 6 0.6717428557274105 2.119070380180844 7 0.4633573409430855 1.7053171606318882 8 0.3331336738649251 1.401198280778743 9 0.24111860500192706 1.1409439407066382 >10 1.4146741055547978 13.887541291702254 >50 0.12628338128628944 4.592058831507079 >100 0.06636096510093786 6.24812764581582 >500 0.0033210780110074813 1.1422769893004914 >1k 3.0893748939584916E-4 0.3034160563117294 >5k 1.5446874469792458E-4 0.4377036497569769 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5763 0.23299703811157174 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5016 0.20279596445733888 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3259 0.13176077515280452 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 8.085963495109205E-5 0.0 0.0 4.0429817475546025E-5 0.0 3 8.085963495109205E-5 0.0 0.0 4.0429817475546025E-5 0.0 4 8.085963495109205E-5 0.0 0.0 4.0429817475546025E-5 0.0 5 8.085963495109205E-5 8.085963495109205E-5 0.0 8.085963495109205E-5 0.0 6 2.0214908737773012E-4 8.085963495109205E-5 0.0 8.085963495109205E-5 0.0 7 3.234385398043682E-4 8.085963495109205E-5 0.0 1.2128945242663807E-4 0.0 8 3.638683572799142E-4 8.085963495109205E-5 0.0 1.2128945242663807E-4 0.0 9 3.638683572799142E-4 8.085963495109205E-5 0.0 1.2128945242663807E-4 0.0 10-11 3.638683572799142E-4 8.085963495109205E-5 0.0 2.2236399611550312E-4 4.0429817475546025E-5 12-13 4.4472799223100625E-4 8.085963495109205E-5 0.0 2.4257890485327615E-4 1.8193417863995713E-4 14-15 4.4472799223100625E-4 1.2128945242663807E-4 0.0 2.627938135910491E-4 2.4257890485327615E-4 16-17 4.649429009687793E-4 1.617192699021841E-4 0.0 4.042981747554602E-4 3.234385398043682E-4 18-19 4.851578097065523E-4 1.617192699021841E-4 0.0 4.851578097065523E-4 3.234385398043682E-4 20-21 4.851578097065523E-4 1.617192699021841E-4 0.0 5.660174446576443E-4 3.840832660176872E-4 22-23 5.255876271820984E-4 1.617192699021841E-4 0.0 8.085963495109204E-4 4.4472799223100625E-4 24-25 5.458025359198714E-4 1.617192699021841E-4 0.0 0.0010511752543641967 4.4472799223100625E-4 26-27 5.862323533954173E-4 1.617192699021841E-4 0.0 0.0012331094330041538 4.4472799223100625E-4 28-29 7.075218058220554E-4 1.617192699021841E-4 0.0 0.0023449294135816693 4.4472799223100625E-4 30-31 7.681665320353744E-4 1.617192699021841E-4 0.0 0.00462921410095002 4.4472799223100625E-4 32-33 7.883814407731475E-4 1.617192699021841E-4 0.0 0.009965950007722095 4.4472799223100625E-4 34-35 8.692410757242396E-4 1.8193417863995713E-4 0.0 0.015949562994102905 4.4472799223100625E-4 36-37 0.0010107454368886507 2.2236399611550312E-4 0.0 0.027007118073664745 4.4472799223100625E-4 38-39 0.0010107454368886507 2.4257890485327615E-4 0.0 0.04788911879978426 4.4472799223100625E-4 40-41 0.0010107454368886507 2.4257890485327615E-4 0.0 0.07158099184045424 4.4472799223100625E-4 42-43 0.0010309603456264238 2.4257890485327615E-4 0.0 0.09575802269083075 4.4472799223100625E-4 44-45 0.0011320348893152886 2.4257890485327615E-4 0.0 0.1215724611489669 4.851578097065523E-4 46-47 0.0012533243417419267 2.4257890485327615E-4 0.0 0.15448233257406135 5.255876271820984E-4 48-49 0.0013341839766930188 2.4257890485327615E-4 0.0 0.19177883919525257 5.660174446576443E-4 50-51 0.0013341839766930188 2.4257890485327615E-4 0.0 0.2334619810125405 6.064472621331903E-4 52-53 0.0013341839766930188 2.4257890485327615E-4 0.0 0.27330556613469115 6.468770796087364E-4 54-55 0.0013341839766930188 2.627938135910491E-4 0.0 0.31682826464711644 6.873068970842825E-4 56-57 0.0014959032465952028 2.8300872232882215E-4 0.0 0.3698115404488195 7.075218058220554E-4 58-59 0.0014959032465952028 3.638683572799142E-4 0.0 0.4310020691980584 7.681665320353744E-4 60-61 0.0014959032465952028 3.638683572799142E-4 0.0 0.49949018000163337 7.681665320353744E-4 62-63 0.0014959032465952028 3.638683572799142E-4 0.0 0.5680187206226839 8.085963495109205E-4 64-65 0.0014959032465952028 3.638683572799142E-4 0.0 0.6360418885252901 8.288112582486935E-4 66-67 0.001617192699021841 4.0429817475546025E-4 0.0 0.7192464528899638 8.490261669864666E-4 68-69 0.001617192699021841 4.0429817475546025E-4 0.0 0.8207859394797976 8.490261669864666E-4 70-71 0.001657622516497387 4.245130834932332E-4 0.0 0.9354044720229706 8.692410757242396E-4 72-73 0.001657622516497387 4.4472799223100625E-4 0.0 1.0527722321544806 9.096708931997855E-4 74-75 0.001657622516497387 4.4472799223100625E-4 0.0 1.1664002341695028 9.501007106753316E-4 76-77 0.001657622516497387 4.4472799223100625E-4 0.0 1.290620848363118 9.703156194131046E-4 78-79 0.001657622516497387 4.4472799223100625E-4 0.0 1.4403931072012783 9.703156194131046E-4 80-81 0.002001275965039528 4.4472799223100625E-4 0.0 1.6116942438451667 9.703156194131046E-4 82-83 0.0021023505087283934 4.4472799223100625E-4 0.0 1.7965393693433631 0.0010916050718397428 84-85 0.0021832101436794855 4.4472799223100625E-4 0.0 1.9756838905775074 0.0011118199805775157 86-87 0.0022842846873683503 4.4472799223100625E-4 0.0 2.1656838178038362 0.0011926796155286076 88-89 0.002324714504843896 4.4472799223100625E-4 0.0 2.3863295466766283 0.0012533243417419267 90-91 0.0024257890485327614 4.4472799223100625E-4 0.0 2.633780244535708 0.0012533243417419267 92-93 0.0024662188660083077 4.4472799223100625E-4 0.0 2.8954824530549175 0.0012533243417419267 94-95 0.0024662188660083077 4.4472799223100625E-4 0.0 3.1683028613799022 0.0012533243417419267 96-97 0.0025875083184349456 4.4472799223100625E-4 0.0 3.4412243442485755 0.0013341839766930188 98-99 0.0026683679533860377 4.4472799223100625E-4 0.0 3.733390420235609 0.0013341839766930188 100-101 0.00274922758833713 4.4472799223100625E-4 0.0 4.07777160549231 0.0013341839766930188 102-103 0.0028705170407637677 4.851578097065523E-4 0.0 4.455183951626532 0.0013948287029063378 104-105 0.003274815215519228 4.851578097065523E-4 0.0 4.826693544409324 0.0014554734291196568 106-107 0.003335459941732547 4.851578097065523E-4 0.0 5.198546790640659 0.0014554734291196568 108-109 0.00335567485047032 4.851578097065523E-4 0.0 5.595769747337899 0.0014554734291196568 110-111 0.0033961046679458658 4.851578097065523E-4 0.0 6.043489546062095 0.0014554734291196568 112-113 0.003436534485421412 4.851578097065523E-4 0.0 6.52345212422304 0.0014554734291196568 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5005 0.0 60.743206 1 GTATCAA 8135 0.0 50.781708 1 ATCAACG 10300 0.0 39.61179 3 TCAACGC 10505 0.0 38.611984 4 CAACGCA 10550 0.0 38.293304 5 TATCAAC 10865 0.0 37.551903 2 AACGCAG 10795 0.0 37.31398 6 GTACATG 10855 0.0 34.76206 1 TACATGG 11070 0.0 33.09014 2 ACATGGG 11035 0.0 32.007626 3 ACGCAGA 13015 0.0 30.674952 7 CGCAGAG 13210 0.0 29.99694 8 TGGTATC 2385 0.0 27.221321 2 GCAGAGT 14140 0.0 26.719053 9 CATGGGG 5290 0.0 26.459602 4 GAGTACT 8570 0.0 26.311546 12-13 GTGGTAT 2730 0.0 25.547878 1 GTACTTT 9215 0.0 24.276188 14-15 CATGGGA 6255 0.0 24.18675 4 CAGAGTA 13815 0.0 24.117102 10-11 >>END_MODULE