FastQCFastQC Report
Thu 26 May 2016
SRR1364587_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364587_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1232253
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39240.31844109935216225No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28500.23128367307687628No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18220.14785924643721704No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16970.13771522568823122No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA63650.046.837541
GGTATCA37250.042.3325421
GTACATG96800.038.2972371
ATCAACG77600.037.1892283
TACATGG96450.036.9540182
TATCAAC79350.036.7439842
CAACGCA79100.036.634445
TCAACGC78750.036.4950264
AACGCAG80750.035.880056
ACATGGG98800.034.750093
CATGGGG34150.031.1888584
ACGCAGA94950.030.3261327
CATGGGA61750.030.064554
CGCAGAG94150.029.8279578
TACCTGG12500.029.0373442
GCAGAGT96500.027.8684629
ATGGGAT23400.027.4628125
CGCGATA557.7627E-427.040125104-105
ATGGGAG24550.026.1763655
GAGTACT40100.026.11804412-13
Produced by FastQC (version 0.11.5)