FastQCFastQC Report
Thu 26 May 2016
SRR1364587_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364587_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1232253
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73670.5978480068622272No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64900.5266775572873428No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43210.35065850925094116No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19690.15978861483802434No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14010.11369418455463286No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46650.059.549441
GTATCAA81650.048.041121
ATCAACG104850.037.2701453
TATCAAC105950.036.770752
CAACGCA106700.036.6923685
TCAACGC106600.036.6024174
GTACATG87800.036.188961
AACGCAG109250.035.672556
TACATGG89000.034.7378582
ACATGGG89850.031.9561613
CGCAGAG127700.030.285648
ACGCAGA128000.030.2611437
TACCTGG11500.030.0438692
CATGGGG32100.029.5065044
GCAGAGT131250.028.8759849
GAGTACT80250.028.54383912-13
GTTCGCG850.00721078327.999837
GTACTTT86600.026.65695414-15
CATGGGA55700.026.2021274
AGTACTT84750.026.11559312-13
Produced by FastQC (version 0.11.5)