##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364587_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1232253 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.76465466101523 31.0 30.0 34.0 26.0 34.0 2 29.648592456256953 31.0 30.0 34.0 25.0 34.0 3 29.90598602722006 31.0 30.0 34.0 26.0 34.0 4 33.38344804191996 35.0 33.0 37.0 28.0 37.0 5 32.781940072371505 35.0 33.0 37.0 26.0 37.0 6 32.790314164380206 35.0 33.0 37.0 25.0 37.0 7 32.55833907484908 35.0 33.0 37.0 24.0 37.0 8 32.46338049085699 35.0 33.0 37.0 23.0 37.0 9 33.61134523511 38.0 33.0 39.0 19.0 39.0 10-11 33.55324758795474 38.0 33.0 39.0 20.0 39.0 12-13 33.425645139431595 37.5 33.0 39.0 18.5 39.0 14-15 34.32439077040186 38.0 33.0 41.0 17.5 41.0 16-17 34.36030912483069 38.5 33.0 41.0 18.5 41.0 18-19 34.432448937028354 39.0 33.0 41.0 19.0 41.0 20-21 34.4863303233995 39.0 33.0 41.0 19.0 41.0 22-23 34.4486030871907 39.0 33.0 41.0 21.0 41.0 24-25 34.41356807408868 39.0 33.0 41.0 21.0 41.0 26-27 34.28090335345095 39.0 33.0 41.0 20.0 41.0 28-29 34.12093620384775 38.0 33.0 41.0 19.0 41.0 30-31 33.913341253784736 38.0 33.0 40.0 18.5 41.0 32-33 33.629506684098146 38.0 33.0 40.0 16.5 41.0 34-35 33.404217721523096 38.0 32.0 40.0 16.0 41.0 36-37 33.15560481492031 38.0 32.0 40.0 14.0 41.0 38-39 32.96421514088422 38.0 32.0 40.0 9.0 41.0 40-41 32.94661161303726 38.0 32.0 40.0 9.0 41.0 42-43 32.764518325376365 38.0 31.5 40.0 7.5 41.0 44-45 32.51818498311629 38.0 31.0 40.0 2.0 41.0 46-47 32.15273446280918 38.0 30.5 40.0 2.0 41.0 48-49 31.75855201813264 38.0 30.0 40.0 2.0 41.0 50-51 31.544806139648273 37.0 30.0 40.0 2.0 41.0 52-53 31.361603907639097 37.0 30.0 40.0 2.0 41.0 54-55 31.16753377756029 37.0 29.5 40.0 2.0 41.0 56-57 30.947497794689887 36.5 29.0 40.0 2.0 41.0 58-59 30.73733478433406 36.0 29.0 40.0 2.0 41.0 60-61 30.50021221291407 36.0 28.5 40.0 2.0 41.0 62-63 30.31667888006765 35.5 28.5 39.5 2.0 41.0 64-65 30.179694429634175 35.0 28.0 39.0 2.0 41.0 66-67 29.888277407318142 35.0 28.0 39.0 2.0 41.0 68-69 29.603779012913744 35.0 28.0 39.0 2.0 40.5 70-71 29.289129342756723 35.0 28.0 38.0 2.0 40.0 72-73 29.00641954209079 35.0 28.0 37.0 2.0 40.0 74-75 28.65924773565169 34.5 27.5 37.0 2.0 39.0 76-77 28.358316027633936 34.0 27.0 36.0 2.0 39.0 78-79 28.078476984839963 34.0 26.5 36.0 2.0 38.5 80-81 27.79697432264316 34.0 26.0 35.5 2.0 37.0 82-83 27.519745539268314 34.0 26.0 35.0 2.0 37.0 84-85 27.30265538002342 34.0 26.0 35.0 2.0 36.5 86-87 27.088263530297755 34.0 26.0 35.0 2.0 36.0 88-89 26.90759973804081 34.0 25.5 35.0 2.0 36.0 90-91 26.761691795434867 34.0 25.0 35.0 2.0 35.5 92-93 26.647133340312422 34.0 25.0 35.0 2.0 35.0 94-95 26.53148460583987 34.0 25.0 35.0 2.0 35.0 96-97 26.412873005786963 34.0 25.0 35.0 2.0 35.0 98-99 26.323701382751757 34.0 24.0 35.0 2.0 35.0 100-101 26.22463284731301 34.0 24.0 35.0 2.0 35.0 102-103 24.993643756598686 31.5 20.0 34.0 2.0 35.0 104-105 25.8789818324646 33.0 23.5 35.0 2.0 35.0 106-107 26.026842701945135 33.0 24.0 35.0 2.0 35.0 108-109 26.029556024615076 34.0 24.0 35.0 2.0 35.0 110-111 25.951031565758008 33.5 23.0 35.0 2.0 35.0 112-113 25.859308924384848 33.0 23.0 35.0 2.0 35.0 114-115 25.755256022910878 33.0 21.5 35.0 2.0 35.0 116-117 25.63262536183722 33.0 20.0 35.0 2.0 35.0 118-119 25.49610388451073 33.0 19.0 35.0 2.0 35.0 120-121 25.29477307014063 33.0 17.5 35.0 2.0 35.0 122-123 25.00659969989929 33.0 2.0 35.0 2.0 35.0 124-125 23.286439148454093 31.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.8282132052282378 1101 2 1.0035754137609345 1101 3 0.8267027957200881 1101 4 0.7755108326789824 1101 5 0.8505542671051884 1101 6 0.8753071943951838 1101 7 1.0690190953754097 1101 8 1.1639354334692769 1101 9 1.4443911478799407 1101 10-11 1.289241657622327 1101 12-13 1.158622743712428 1101 14-15 1.2956874483387324 1101 16-17 1.3974971356235528 1101 18-19 1.4743566634636238 1101 20-21 1.4734684502855444 1101 22-23 1.3809989205959923 1101 24-25 1.4307178060022068 1101 26-27 1.427901459908938 1101 28-29 1.4268016575660596 1101 30-31 1.566233632713029 1101 32-33 1.6183287492543528 1101 34-35 1.6025255327843269 1101 36-37 1.558227559471554 1101 38-39 1.618542994837739 1101 40-41 1.430085779533428 1101 42-43 1.24280121441506 1101 44-45 1.289034897445724 1101 46-47 1.4059331122070873 1101 48-49 1.3562314531888013 1101 50-51 1.3275144371682934 1101 52-53 1.3834673988746147 1101 54-55 1.2930127568164167 1101 56-57 1.270239065218366 1101 58-59 1.3578367760125118 1101 60-61 1.2783025979091818 1101 62-63 1.3077602714159795 1101 64-65 1.2434562014180273 1101 66-67 1.4189885068203658 1101 68-69 1.5058327931330382 1101 70-71 1.459460247006696 1101 72-73 1.25251423211623 1101 74-75 1.2381348556786058 1101 76-77 1.1967288087857426 1101 78-79 1.3071731171854353 1101 80-81 1.268658553522382 1101 82-83 1.4463097172219186 1101 84-85 1.4126847855920097 1101 86-87 1.375301525217079 1101 88-89 1.2957777195392168 1101 90-91 1.3243159393806465 1101 92-93 1.2843436122644505 1101 94-95 1.243559009192026 1101 96-97 1.197310850959699 1101 98-99 1.2161040136704315 1101 100-101 1.3255142957329156 1101 102-103 1.2314777974990925 1101 104-105 1.2178109010790124 1101 106-107 1.1210627367802388 1101 108-109 1.089710687292353 1101 110-111 1.1703567040105227 1101 112-113 1.2108328353407067 1101 114-115 1.3005734393514068 1101 116-117 1.2524096977176704 1101 118-119 1.2303309932616528 1101 120-121 1.2086985266245733 1101 122-123 1.19129743722905 1101 124-125 1.345865248412398 1102 1 0.30633842654692245 1102 2 -0.3362529091573485 1102 3 0.15132540958081364 1102 4 0.17260592241272832 1102 5 0.30062218135186214 1102 6 0.09975096836598141 1102 7 0.17654528367319955 1102 8 0.18449424766752998 1102 9 0.37876393289378285 1102 10-11 0.5494988968664529 1102 12-13 0.46186057485555665 1102 14-15 0.6410708341935702 1102 16-17 0.6248811732709072 1102 18-19 0.6700294405891611 1102 20-21 0.5360913451310338 1102 22-23 0.604600028182638 1102 24-25 0.6341974107119199 1102 26-27 0.664722670112603 1102 28-29 0.32445209525164387 1102 30-31 0.5416450191597733 1102 32-33 0.744170767876497 1102 34-35 0.8264607416141629 1102 36-37 0.4669488254691103 1102 38-39 -0.07700699540264822 1102 40-41 -0.44710660693116466 1102 42-43 -0.06070320677648766 1102 44-45 -0.48939710395459457 1102 46-47 -0.39700761210022506 1102 48-49 -0.662130008369548 1102 50-51 -0.8305259589971818 1102 52-53 -1.0047569501821485 1102 54-55 -0.9470166026362143 1102 56-57 -0.8967762893320064 1102 58-59 -0.6947741654649278 1102 60-61 -0.8029934039886157 1102 62-63 -0.6508994140077604 1102 64-65 -0.7057296049362591 1102 66-67 -0.784150087184873 1102 68-69 -0.5671175871464378 1102 70-71 -0.9992915645676881 1102 72-73 -0.7450100215323978 1102 74-75 -0.7335563616475618 1102 76-77 -1.0760456393354296 1102 78-79 -0.894461085569251 1102 80-81 -0.8208984290928356 1102 82-83 -1.1101274823964502 1102 84-85 -0.9106378419149017 1102 86-87 -0.6350724551335638 1102 88-89 -0.8560961263648252 1102 90-91 -0.8156531926010047 1102 92-93 -0.7718209515268661 1102 94-95 -0.4402328052843316 1102 96-97 -0.7577334588398195 1102 98-99 -0.9758421167145599 1102 100-101 -0.8102730287451756 1102 102-103 -0.9309102332411001 1102 104-105 -0.8529781133381533 1102 106-107 -0.6890652667481909 1102 108-109 -0.6019956272067475 1102 110-111 -0.5435304207265617 1102 112-113 -0.38416434041699077 1102 114-115 -0.5761296708850203 1102 116-117 -0.7375793565440567 1102 118-119 -0.4932852287715832 1102 120-121 -0.31652431789699875 1102 122-123 -0.4208432635860895 1102 124-125 -0.03359689608861771 1103 1 0.44829651393327197 1103 2 0.027456684915712515 1103 3 0.2514556298331634 1103 4 0.48301586521621687 1103 5 0.5339142733505469 1103 6 0.49322637268362257 1103 7 0.5913892113049926 1103 8 0.6281372256659168 1103 9 0.9145003860012011 1103 10-11 0.7995052454728651 1103 12-13 0.6822489028688565 1103 14-15 0.703091551320334 1103 16-17 0.7587747588161591 1103 18-19 0.8579112797361432 1103 20-21 0.9303062882740889 1103 22-23 1.022813589396499 1103 24-25 0.894444504378967 1103 26-27 1.029445236012613 1103 28-29 1.0054935324580185 1103 30-31 1.0185129120425387 1103 32-33 1.0737772723928742 1103 34-35 0.9982686475484854 1103 36-37 1.0138291498090766 1103 38-39 1.0269753525187042 1103 40-41 0.9954309753423445 1103 42-43 0.8244468122693291 1103 44-45 0.9117844750471633 1103 46-47 1.197339322999298 1103 48-49 0.9213215369993684 1103 50-51 0.9006133648614671 1103 52-53 0.893882381058603 1103 54-55 0.9286478602273931 1103 56-57 1.011041650353281 1103 58-59 1.0377464970342984 1103 60-61 1.162515207969797 1103 62-63 0.9305781576945797 1103 64-65 0.9039623547751923 1103 66-67 0.8687364617422126 1103 68-69 0.8388707727575344 1103 70-71 0.7787921602630412 1103 72-73 0.7689453964864228 1103 74-75 0.8680055431705895 1103 76-77 0.753996852034895 1103 78-79 0.6827183319534917 1103 80-81 0.780278277722612 1103 82-83 0.940727364838363 1103 84-85 0.9201574760616928 1103 86-87 0.9707421301252666 1103 88-89 0.9889853277317968 1103 90-91 0.9466247169062534 1103 92-93 0.8709132899032568 1103 94-95 0.9192416777471699 1103 96-97 0.9078956289983182 1103 98-99 1.0076228640772165 1103 100-101 0.9442997395720312 1103 102-103 0.9466381416632998 1103 104-105 0.8279810872689524 1103 106-107 1.0171504618503633 1103 108-109 0.95898628074141 1103 110-111 0.8830545900660702 1103 112-113 1.0093314384979628 1103 114-115 0.9807296292328012 1103 116-117 1.0989449143258803 1103 118-119 1.0000370707822448 1103 120-121 0.9967810323541393 1103 122-123 1.0483159446983983 1103 124-125 1.2435968427090422 1104 1 0.5105945880205383 1104 2 0.70303526560539 1104 3 0.40733547869826126 1104 4 0.23661756131041045 1104 5 0.5489637705877186 1104 6 0.706884160081998 1104 7 0.7227291145920134 1104 8 0.5049405777890996 1104 9 0.47751184354041953 1104 10-11 0.616019627216474 1104 12-13 0.5709674836698255 1104 14-15 0.6361381215707951 1104 16-17 0.6120484209439638 1104 18-19 0.5694291950093344 1104 20-21 0.5676721115308396 1104 22-23 0.625028862034732 1104 24-25 0.7108873808389831 1104 26-27 0.5988437569383436 1104 28-29 0.7289325192531919 1104 30-31 0.6375238576373121 1104 32-33 0.7655196641750521 1104 34-35 0.9250177446899457 1104 36-37 0.9539102262279116 1104 38-39 1.03114408272954 1104 40-41 0.8634149715138904 1104 42-43 0.9003550963094682 1104 44-45 0.7680480040137638 1104 46-47 0.8191125427403207 1104 48-49 0.7781999557587902 1104 50-51 0.6955687644841895 1104 52-53 0.7793668084876941 1104 54-55 0.4376477878874141 1104 56-57 0.6195799933226702 1104 58-59 0.7282872105290394 1104 60-61 0.803689452712625 1104 62-63 0.8379081448403234 1104 64-65 0.734236356344411 1104 66-67 0.7572850436004579 1104 68-69 0.4865915070639346 1104 70-71 0.24663286763671266 1104 72-73 0.2170812509606499 1104 74-75 0.6064205743046038 1104 76-77 0.7761776797155093 1104 78-79 0.6338827273304126 1104 80-81 0.7609526532338649 1104 82-83 0.6760807456193021 1104 84-85 0.7009706886800124 1104 86-87 0.7408506957348422 1104 88-89 0.6938743971614798 1104 90-91 0.6958595825486604 1104 92-93 0.6486392549463531 1104 94-95 0.584543808437683 1104 96-97 0.6666226123656891 1104 98-99 0.8166659174919388 1104 100-101 0.8048054516477947 1104 102-103 0.6677486159353094 1104 104-105 0.7519108729756852 1104 106-107 0.6467007854326354 1104 108-109 0.6601431638900941 1104 110-111 0.7127064678448995 1104 112-113 0.717653318001819 1104 114-115 0.6406535609661184 1104 116-117 0.5142887796177114 1104 118-119 0.5019078938442298 1104 120-121 0.5553983928353645 1104 122-123 0.6338215176588271 1104 124-125 1.0354352240742628 1105 1 0.2954022461783232 1105 2 0.08140796239640125 1105 3 0.37958522297823905 1105 4 0.39962780504797024 1105 5 -0.03749642809111009 1105 6 0.3054288932452778 1105 7 0.5202505804140429 1105 8 0.592930670954722 1105 9 0.7283697295919112 1105 10-11 0.7295674777022754 1105 12-13 0.6822160667679142 1105 14-15 0.7545113217803561 1105 16-17 0.6577177490904234 1105 18-19 0.6720589483729782 1105 20-21 0.6387795659424143 1105 22-23 0.8515670684595733 1105 24-25 0.7858508276738334 1105 26-27 0.7417118651523111 1105 28-29 0.6351038773934405 1105 30-31 0.8327497072390884 1105 32-33 0.839310311764919 1105 34-35 0.8851263890501926 1105 36-37 0.9786053952354763 1105 38-39 0.8588122978231354 1105 40-41 0.6431011757989253 1105 42-43 0.6288609224109933 1105 44-45 0.7405848628258838 1105 46-47 0.718221519578087 1105 48-49 0.6243909306260313 1105 50-51 0.23144646365122767 1105 52-53 0.4672007036029342 1105 54-55 0.3278910662817438 1105 56-57 0.3943046206131555 1105 58-59 0.0011498807163192737 1105 60-61 0.23133811193201836 1105 62-63 0.4091111657941511 1105 64-65 0.1580840672560342 1105 66-67 0.3700624147709526 1105 68-69 0.4252672452221198 1105 70-71 0.4319491168255709 1105 72-73 0.26750373529243987 1105 74-75 0.28056300634724707 1105 76-77 0.39330948298839274 1105 78-79 0.2920046940262324 1105 80-81 0.25450350210264006 1105 82-83 0.22642177019221066 1105 84-85 0.3145769025244647 1105 86-87 0.3817922599060459 1105 88-89 0.39675497466553367 1105 90-91 0.3610710376993218 1105 92-93 0.4594472361771409 1105 94-95 0.36313729021863494 1105 96-97 0.4498179481652187 1105 98-99 0.43010464785930935 1105 100-101 0.47213693505628385 1105 102-103 0.24989047928096397 1105 104-105 0.40614351651497316 1105 106-107 0.4220696224970766 1105 108-109 0.38945349154917963 1105 110-111 0.4340912726816981 1105 112-113 0.37942244140630166 1105 114-115 0.3674982441747865 1105 116-117 0.04158180959435143 1105 118-119 0.32550381250285554 1105 120-121 0.4342814232601029 1105 122-123 0.496699157632694 1105 124-125 1.165897246123997 1106 1 -0.17807433969269226 1106 2 -0.43386163045023807 1106 3 -0.006870078964507087 1106 4 -0.14703124588984195 1106 5 0.14168732170620757 1106 6 0.26339521115901476 1106 7 0.2890637752857401 1106 8 0.2820507435414257 1106 9 0.18619678362121306 1106 10-11 0.20788920382869236 1106 12-13 0.2129589223611461 1106 14-15 0.18085442731413082 1106 16-17 0.15663202750858574 1106 18-19 0.13664962272090975 1106 20-21 0.19661673284608838 1106 22-23 0.24644529306819862 1106 24-25 0.271380236646948 1106 26-27 0.49301312090012317 1106 28-29 0.626982504822152 1106 30-31 0.5151293230741558 1106 32-33 0.5901095960671938 1106 34-35 0.6121056169802586 1106 36-37 0.4979094034930043 1106 38-39 0.6721170552243194 1106 40-41 0.669868796070098 1106 42-43 0.6860174476725547 1106 44-45 0.6928772367581715 1106 46-47 0.5360193027682314 1106 48-49 0.6281702643175571 1106 50-51 0.6749619643147717 1106 52-53 0.5105832397423313 1106 54-55 0.6015160684709819 1106 56-57 0.31120645398123514 1106 58-59 0.6261409118961758 1106 60-61 0.5816278825053622 1106 62-63 0.5776869906007498 1106 64-65 0.567305765755659 1106 66-67 0.27586762838141254 1106 68-69 0.3035669942081505 1106 70-71 0.42890127163051517 1106 72-73 0.5178807082210071 1106 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2216 120-121 -2.2614793218427494 2216 122-123 -2.361357624766196 2216 124-125 -2.49769464444158 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 47246.0 3 15393.0 4 7237.0 5 5688.0 6 6519.0 7 7903.0 8 8489.0 9 9274.0 10 10309.0 11 11613.0 12 13195.0 13 15549.0 14 19154.0 15 16682.0 16 9994.0 17 8290.0 18 7342.0 19 6915.0 20 6790.0 21 6903.0 22 7603.0 23 9478.0 24 12568.0 25 16927.0 26 20206.0 27 21267.0 28 24129.0 29 28874.0 30 32939.0 31 38525.0 32 46440.0 33 58088.0 34 74854.0 35 103094.0 36 160507.0 37 244796.0 38 90972.0 39 501.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.22991788530911 19.498279157457297 13.655862358589957 22.615940598643633 2 15.452533615310518 21.233579846962996 39.83888022813534 23.475006309591144 3 19.423852082324004 27.03900903467064 29.160326653698547 24.376812229306807 4 12.124161079277297 17.499264852816225 36.58092386474592 33.79565020316056 5 12.274102801940835 40.01118276847368 33.23578842981109 14.478925999774397 6 30.23315828811129 37.824781112320274 17.42117892997623 14.520881669592203 7 27.53154849338192 34.407781014907926 20.19030538130057 17.870365110409583 8 26.753645583098518 35.65261780784563 18.93933983456909 18.654396774486763 9 27.513595502061744 15.912235667812846 19.50212991464922 37.07203891547619 10-11 24.961117763369586 27.92911158603413 26.146229670767983 20.963540979828306 12-13 25.944631500187054 25.546133789083896 27.481612947990385 21.027621762738658 14-15 22.96210515381594 25.84414551568997 26.662403469420216 24.531345861073873 16-17 21.335330223302993 29.50230452517936 27.056208990143638 22.106156261374014 18-19 20.979460798276943 26.918900626425767 31.205928443495484 20.8957101318018 20-21 22.005813811942172 25.824394313436205 31.575109678487433 20.59468219613419 22-23 21.668683297991564 25.791010449964414 31.401262565398504 21.13904368664552 24-25 21.22031758088639 26.098049669994715 31.659488757584686 21.022143991534207 26-27 21.10163253812326 26.238037156330723 31.534757878455157 21.125572427090866 28-29 20.808429762394574 26.13753019874977 31.892436050064394 21.161603988791263 30-31 20.981202723791302 26.007118668000807 31.899618016754676 21.112060591453215 32-33 20.97207310511721 26.17550941243397 31.623700652382265 21.228716830066553 34-35 21.282804748700144 26.155870588263934 31.53849087809078 21.022833784945135 36-37 21.44157896146327 25.98508585493401 31.261477959477478 21.31185722412524 38-39 21.26564106559286 26.242297645045294 31.296211086522003 21.19585020283984 40-41 21.331212015714303 26.074515541857068 31.41846682458878 21.175805617839842 42-43 21.35417807869001 26.186343226593888 30.937275056339892 21.52220363837621 44-45 21.466654980754765 26.42140047538939 30.606011914761012 21.505932629094836 46-47 21.579253611068506 26.754124355956122 29.820621252291534 21.846000780683834 48-49 21.94330222770811 26.957329379599805 29.2588453832127 21.840523009479384 50-51 21.868520506746584 27.14612989377993 28.914029829913172 22.07131976956031 52-53 22.00169121113927 27.335498473121994 28.76836169195774 21.894448623780992 54-55 21.740705845309364 27.20305813822324 28.895932896896987 22.16030311957041 56-57 21.72631450802958 27.483022734420175 28.716342203240234 22.074320554310003 58-59 21.85302661365225 27.04952389685967 28.902566741070252 22.194882748417825 60-61 21.953770857121064 27.179321129670615 29.050243740530558 21.81666427267777 62-63 21.74784723591665 27.101536778567386 29.210356964032545 21.940259021483413 64-65 21.630747968858604 26.86438349994583 29.157180297350255 22.347688233845307 66-67 21.626727628173757 27.372219828233323 28.704413785156135 22.29663875843678 68-69 21.665640091766868 27.147874665348752 28.915125384154067 22.27135985873031 70-71 21.466086915592822 27.283804543385166 28.90591461331399 22.344193927708027 72-73 21.76375306045106 27.204234844630122 28.70567164372901 22.326340451189814 74-75 21.601804850535817 27.443385393208274 28.569834487739758 22.384975268516154 76-77 21.664301081028004 27.269643490419583 28.758582855955716 22.307472572596698 78-79 21.63622243159481 27.324624082879083 28.620258989022545 22.418894496503558 80-81 21.449265247692843 27.638096752936896 28.48475798781418 22.427880011556077 82-83 21.59422618569401 27.605329425045017 28.35594638438697 22.444498004874 84-85 21.56870382948956 27.43799365876975 28.21255050707931 22.78075200466138 86-87 21.5123030741252 27.19668769319287 28.62289643441728 22.66811279826464 88-89 21.609586803821184 27.09478017797734 28.50636099998742 22.789272018214053 90-91 21.41508277926692 27.433327409225218 28.590679024518508 22.56091078698936 92-93 21.47954190221599 27.227919229102454 28.505201156281395 22.78733771240016 94-95 21.29043305230338 27.16601217444794 28.525392106978032 23.018162666270644 96-97 21.557995714842228 27.05415692767173 28.396028113232045 22.991819244253993 98-99 21.15243296498447 27.07958583375162 28.334606789992456 23.43337441127145 100-101 21.068518486207317 27.175213708160694 28.448109706836266 23.30815809879572 102-103 21.500657738305364 26.9013749611484 28.359638807939604 23.238328492606634 104-105 21.332875635117137 26.841728119144364 28.592829556917287 23.23256668882121 106-107 21.150327677875463 27.07725780423565 28.477359976595306 23.29505454129358 108-109 21.136568545582765 27.245297840622012 27.966294259376927 23.65183935441829 110-111 20.90777218050757 27.129979975613665 28.382893858579543 23.57935398529922 112-113 20.957668595653654 27.08802494292974 28.373596980490206 23.5807094809264 114-115 20.79443101376097 27.200461268911496 28.151240045672438 23.85386767165509 116-117 20.68852743714156 27.041240719235415 28.271223523091443 23.99900832053158 118-119 20.724396694509974 26.95383983646216 28.179359271188627 24.142404197839245 120-121 20.508990722199567 27.024307309640754 28.34855963822056 24.118142329939115 122-123 20.562020749125647 27.01419781666317 28.154153984368563 24.269627449842616 124-125 20.29208266971828 26.888084654675165 28.1660427805315 24.653789895075047 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 749.0 1 545.5 2 718.5 3 1152.5 4 821.0 5 487.0 6 899.0 7 2100.0 8 3302.5 9 3573.0 10 2687.0 11 1648.0 12 1415.0 13 1478.5 14 1616.0 15 1837.0 16 2057.5 17 2360.0 18 2665.0 19 2926.0 20 3293.0 21 3795.0 22 4400.5 23 5004.0 24 5797.0 25 7029.5 26 8920.5 27 10843.5 28 12462.0 29 14220.0 30 16469.0 31 19070.0 32 21831.0 33 24526.0 34 27481.5 35 29762.0 36 32467.0 37 35595.5 38 36442.0 39 36769.5 40 36497.5 41 36597.5 42 37599.0 43 38577.5 44 40283.0 45 41432.0 46 41296.0 47 40764.5 48 42294.0 49 43640.0 50 43305.0 51 41993.0 52 39696.0 53 38814.0 54 37946.5 55 34706.5 56 30949.0 57 28107.0 58 26001.0 59 23888.5 60 21023.5 61 17480.5 62 13860.0 63 10704.5 64 8128.5 65 6213.5 66 4752.5 67 3781.0 68 2844.5 69 1936.0 70 1380.5 71 1136.0 72 959.5 73 824.0 74 672.0 75 511.5 76 332.5 77 183.5 78 113.5 79 67.0 80 44.0 81 26.0 82 17.5 83 12.0 84 4.5 85 2.5 86 3.0 87 2.5 88 1.0 89 1.5 90 1.5 91 1.0 92 1.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0949480342105071 2 1.6230433198377283E-4 3 0.0 4 0.0979506643522069 5 0.0 6 0.0 7 0.0018664998178133875 8 0.006248716781375253 9 0.003570695303643002 10-11 5.274890789472616E-4 12-13 0.0 14-15 8.115216599188641E-5 16-17 2.840325809716024E-4 18-19 0.001947651983805274 20-21 0.001217282489878296 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.007344271022265719 58-59 7.709455769229209E-4 60-61 0.0 62-63 0.0 64-65 2.0288041497971602E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0014607389878539552 76-77 0.0 78-79 0.0 80-81 8.115216599188641E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0014201629048580123 90-91 0.0 92-93 0.002191108481780933 94-95 0.0 96-97 0.007547151437245436 98-99 0.003448967054655172 100-101 0.0019070759008093306 102-103 0.0 104-105 0.0 106-107 0.0017447715688255578 108-109 0.0 110-111 4.4633691295537523E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 8.520977429148073E-4 122-123 0.0045850973785415815 124-125 0.08050294866395132 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1232253.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.0956603508041 #Duplication Level Percentage of deduplicated Percentage of total 1 80.17007619157307 45.773634405321424 2 11.088741947897834 12.66238087949777 3 3.5927350597994088 6.153887421141986 4 1.6134982741273767 3.6849499774474124 5 0.8967621459896196 2.5600613451440766 6 0.5476108276823586 1.8759721093064792 7 0.36050256697958255 1.4408192483901474 8 0.25497749970928146 1.164648697639871 9 0.19736197364495933 1.0141660992057255 >10 1.1203179169433037 12.109072088425254 >50 0.10612772545169459 4.191177057346194 >100 0.049002064270944036 4.971035220272616 >500 0.0012857658359496686 0.4460165547195037 >1k 7.143143533053715E-4 0.8218841999549595 >5k 2.8572574132214854E-4 1.1302946961865696 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7367 0.5978480068622272 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6490 0.5266775572873428 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4321 0.35065850925094116 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1969 0.15978861483802434 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1401 0.11369418455463286 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 8.115216599188641E-5 0.0 0.0 0.0 0.0 3 8.115216599188641E-5 0.0 0.0 0.0 0.0 4 8.115216599188641E-5 0.0 0.0 0.0 0.0 5 8.115216599188641E-5 0.0 0.0 0.0 0.0 6 8.115216599188641E-5 0.0 0.0 0.0 0.0 7 1.6230433198377283E-4 0.0 0.0 0.0 0.0 8 1.6230433198377283E-4 0.0 0.0 0.0 0.0 9 1.6230433198377283E-4 0.0 0.0 0.0 0.0 10-11 1.6230433198377283E-4 0.0 0.0 1.2172824898782962E-4 0.0 12-13 1.6230433198377283E-4 0.0 0.0 1.6230433198377283E-4 0.0 14-15 1.6230433198377283E-4 0.0 0.0 2.434564979756592E-4 0.0 16-17 2.0288041497971602E-4 0.0 0.0 3.6518474696348884E-4 0.0 18-19 3.2460866396754565E-4 0.0 0.0 4.4633691295537523E-4 0.0 20-21 4.0576082995943204E-4 0.0 0.0 7.709455769229209E-4 0.0 22-23 4.0576082995943204E-4 0.0 0.0 0.0011361303238864098 0.0 24-25 5.680651619432048E-4 0.0 0.0 0.001785347651821501 0.0 26-27 6.08641244939148E-4 0.0 0.0 0.0021911084817809326 0.0 28-29 7.709455769229209E-4 0.0 0.0 0.00316493447368357 0.0 30-31 8.926738259107505E-4 0.0 0.0 0.006126988532387424 0.0 32-33 9.332499089066937E-4 0.0 0.0 0.011564183653843813 0.0 34-35 0.0010144020748985801 0.0 0.0 0.018664998178133874 0.0 36-37 0.0010549781578945232 0.0 0.0 0.03177107298582353 0.0 38-39 0.001217282489878296 0.0 0.0 0.05514289679148682 0.0 40-41 0.0012984346558701826 0.0 0.0 0.08334327447366734 0.0 42-43 0.0014201629048580123 0.0 0.0 0.11097558699390467 0.0 44-45 0.0014607389878539554 0.0 0.0 0.13714716052628803 0.0 46-47 0.001582467236841785 0.0 0.0 0.1765871131983448 0.0 48-49 0.0016230433198377281 0.0 0.0 0.22187002182181742 0.0 50-51 0.0016230433198377281 0.0 0.0 0.2717786039068276 0.0 52-53 0.0016636194028336712 0.0 0.0 0.3173455451112718 0.0 54-55 0.0017041954858296145 0.0 0.0 0.3646166818015456 0.0 56-57 0.0017041954858296145 0.0 0.0 0.42604887145740367 0.0 58-59 0.0017041954858296145 0.0 0.0 0.5045635920545537 0.0 60-61 0.0017041954858296145 0.0 0.0 0.5931817573176936 0.0 62-63 0.0017041954858296145 0.0 0.0 0.6796493901820486 0.0 64-65 0.0017041954858296145 0.0 0.0 0.7674154577022738 0.0 66-67 0.0022722606477728192 0.0 0.0 0.8639459591496227 0.0 68-69 0.0022722606477728192 0.0 0.0 0.9846598060625537 0.0 70-71 0.0023128367307687627 0.0 0.0 1.1249719010625254 0.0 72-73 0.002353412813764706 0.0 0.0 1.2672316480463022 0.0 74-75 0.002353412813764706 0.0 0.0 1.403851319493643 0.0 76-77 0.002353412813764706 0.0 0.0 1.5489919683701316 0.0 78-79 0.002353412813764706 0.0 0.0 1.7282976791292048 0.0 80-81 0.002840325809716024 0.0 0.0 1.9347082133295679 0.0 82-83 0.002840325809716024 0.0 0.0 2.160514115201992 0.0 84-85 0.002962054058703854 0.0 0.0 2.375891963744458 0.0 86-87 0.0030837823076916835 0.0 0.0 2.600561735292996 0.0 88-89 0.0030837823076916835 0.0 0.0 2.855460688673511 0.0 90-91 0.0033678148886632855 0.0 0.0 3.1460260190074605 0.0 92-93 0.003408390971659229 0.0 0.0 3.4647917270235897 0.0 94-95 0.003408390971659229 0.0 0.0 3.7805953809810164 0.0 96-97 0.003408390971659229 0.0 0.0 4.097454013096337 0.0 98-99 0.0036924235526308315 0.0 0.0 4.440524794827036 0.0 100-101 0.0039358800506064905 0.0 0.0 4.852615493733836 0.0 102-103 0.004138760465586207 0.0 0.0 5.294610765808645 0.0 104-105 0.004382216963561866 0.0 0.0 5.731006538430014 0.0 106-107 0.004666249544533469 0.0 0.0 6.165089474320615 0.0 108-109 0.004787977793521298 0.0 0.0 6.604487877083683 0.0 110-111 0.00519373862348073 0.0 0.0 7.121751783115967 0.0 112-113 0.005274890789472617 0.0 0.0 7.693387640362815 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4665 0.0 59.54944 1 GTATCAA 8165 0.0 48.04112 1 ATCAACG 10485 0.0 37.270145 3 TATCAAC 10595 0.0 36.77075 2 CAACGCA 10670 0.0 36.692368 5 TCAACGC 10660 0.0 36.602417 4 GTACATG 8780 0.0 36.18896 1 AACGCAG 10925 0.0 35.67255 6 TACATGG 8900 0.0 34.737858 2 ACATGGG 8985 0.0 31.956161 3 CGCAGAG 12770 0.0 30.28564 8 ACGCAGA 12800 0.0 30.261143 7 TACCTGG 1150 0.0 30.043869 2 CATGGGG 3210 0.0 29.506504 4 GCAGAGT 13125 0.0 28.875984 9 GAGTACT 8025 0.0 28.543839 12-13 GTTCGCG 85 0.007210783 27.99983 7 GTACTTT 8660 0.0 26.656954 14-15 CATGGGA 5570 0.0 26.202127 4 AGTACTT 8475 0.0 26.115593 12-13 >>END_MODULE