FastQCFastQC Report
Thu 26 May 2016
SRR1364586_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364586_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2518457
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34260.13603567581261067No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA74300.050.9301451
GGTATCA41750.047.741381
GTACATG132950.041.6646731
ATCAACG90200.041.4906963
TCAACGC91100.041.0154884
CAACGCA92900.040.156745
TACATGG136000.039.7240522
AACGCAG93050.039.511816
TATCAAC96550.038.7618942
ACATGGG140700.037.593633
ACGCAGA110800.032.859927
CATGGGA86950.032.6403924
CGCAGAG110200.032.1782658
CATGGGG55650.029.2948974
GCAGAGT119350.028.3160389
GTATCGA850.007212815327.9993321
ATGGGAG39950.025.4674785
GAGTACT47850.024.12655812-13
GAGTACA101500.023.8580511
AGTACAT93900.023.4445932
Produced by FastQC (version 0.11.5)