FastQCFastQC Report
Thu 26 May 2016
SRR1364586_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364586_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2518457
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88520.351485056127621No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77020.3058221760387412No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52420.20814331950078957No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60300.058.6226161
GTATCAA104300.047.380321
ATCAACG136050.036.594213
TCAACGC136250.036.453084
CAACGCA138250.035.806135
AACGCAG140700.035.224936
TATCAAC139500.035.0915262
GTACATG124050.034.1665081
TACATGG127850.032.6994932
ACATGGG131350.030.5586743
ACGCAGA162050.030.2169067
CGCAGAG164100.029.6943998
GCAGAGT178700.026.8016749
GTGGTAT32300.026.3913331
TGGTATC32900.025.8847982
GAGTACT105000.025.61177812-13
CATGGGG56350.025.3642484
AGTACTT107550.024.70026612-13
CATGGGA80700.024.2049944
GTACTTT114150.023.74122214-15
Produced by FastQC (version 0.11.5)