##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364586_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2518457 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.96850809841105 31.0 30.0 34.0 26.0 34.0 2 29.878809127970023 31.0 30.0 34.0 26.0 34.0 3 30.146935206755565 31.0 30.0 34.0 26.0 34.0 4 33.684150652562266 35.0 35.0 37.0 28.0 37.0 5 33.11126018828195 35.0 33.0 37.0 26.0 37.0 6 33.13228218706931 35.0 33.0 37.0 26.0 37.0 7 32.90684335686494 35.0 33.0 37.0 26.0 37.0 8 32.83443473523669 35.0 33.0 37.0 25.0 37.0 9 34.066386680415825 38.0 34.0 39.0 25.0 39.0 10-11 33.99458875017521 38.0 34.0 39.0 25.0 39.0 12-13 33.87190152541814 38.0 33.0 39.0 24.0 39.0 14-15 34.817193424386446 39.0 34.0 41.0 23.5 41.0 16-17 34.82124253064475 39.0 34.0 41.0 24.0 41.0 18-19 34.83411926429556 39.0 34.0 41.0 24.0 41.0 20-21 34.859741500450475 39.0 34.0 41.0 24.0 41.0 22-23 34.814901346340235 39.0 34.0 41.0 24.0 41.0 24-25 34.762736270660966 39.0 34.0 41.0 24.0 41.0 26-27 34.63673233253536 39.0 34.0 41.0 23.0 41.0 28-29 34.475745267836615 39.0 33.0 41.0 23.0 41.0 30-31 34.29504494220072 38.0 33.0 40.5 22.0 41.0 32-33 34.08107126704962 38.0 33.0 40.0 20.5 41.0 34-35 33.88233251550453 38.0 33.0 40.0 19.0 41.0 36-37 33.65933029628856 38.0 33.0 40.0 17.0 41.0 38-39 33.48313788958875 38.0 32.5 40.0 16.0 41.0 40-41 33.49364432269441 38.0 33.0 40.0 15.5 41.0 42-43 33.343302863618476 38.0 32.5 40.0 14.5 41.0 44-45 33.157407094899774 38.0 32.0 40.0 10.5 41.0 46-47 32.88346535199926 38.0 32.0 40.0 7.5 41.0 48-49 32.58031604271981 38.0 31.0 40.0 4.0 41.0 50-51 32.40098302254118 38.0 31.0 40.0 2.0 41.0 52-53 32.213843833744235 37.0 31.0 40.0 2.0 41.0 54-55 32.0225626246547 37.0 31.0 40.0 2.0 41.0 56-57 31.802519161534224 37.0 30.0 40.0 2.0 41.0 58-59 31.597451534808812 36.5 30.0 40.0 2.0 41.0 60-61 31.36269370491535 36.0 30.0 40.0 2.0 41.0 62-63 31.19978740951305 36.0 30.0 40.0 2.0 41.0 64-65 31.06840100903053 35.5 30.0 39.0 2.0 41.0 66-67 30.775276687273198 35.0 29.5 39.0 2.0 41.0 68-69 30.497597933973065 35.0 29.0 39.0 2.0 41.0 70-71 30.188425889344153 35.0 29.0 38.0 2.0 40.0 72-73 29.909577570710958 35.0 29.0 37.0 2.0 40.0 74-75 29.564793442969247 35.0 29.0 37.0 2.0 39.0 76-77 29.25580960882 35.0 29.0 36.0 2.0 39.0 78-79 28.967631371113345 34.0 29.0 36.0 2.0 38.5 80-81 28.69037291484429 34.0 28.5 35.5 2.0 37.0 82-83 28.40834705535969 34.0 28.0 35.0 2.0 37.0 84-85 28.18872785995552 34.0 28.0 35.0 2.0 36.5 86-87 27.971258790600753 34.0 27.5 35.0 2.0 36.0 88-89 27.793156682841914 34.0 27.0 35.0 2.0 36.0 90-91 27.655045728396395 34.0 27.0 35.0 2.0 35.5 92-93 27.541149402193483 34.0 27.0 35.0 2.0 35.0 94-95 27.429870750225234 34.0 27.0 35.0 2.0 35.0 96-97 27.31143493813871 34.0 26.5 35.0 2.0 35.0 98-99 27.229838746502324 34.0 26.0 35.0 2.0 35.0 100-101 27.130050066370003 34.0 26.0 35.0 2.0 35.0 102-103 25.860271785462288 31.5 24.0 34.0 2.0 35.0 104-105 26.796504566089475 33.5 25.0 35.0 2.0 35.0 106-107 26.95709734174536 34.0 25.0 35.0 2.0 35.0 108-109 26.95738005453339 34.0 25.0 35.0 2.0 35.0 110-111 26.878564335225896 34.0 25.0 35.0 2.0 35.0 112-113 26.79699851933148 34.0 25.0 35.0 2.0 35.0 114-115 26.695550092775058 34.0 25.0 35.0 2.0 35.0 116-117 26.567577091846317 34.0 25.0 35.0 2.0 35.0 118-119 26.42387858915201 34.0 25.0 35.0 2.0 35.0 120-121 26.224089789899132 34.0 24.5 35.0 2.0 35.0 122-123 25.927094844184353 34.0 23.5 35.0 2.0 35.0 124-125 24.122710651799892 31.5 11.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7474268133507351 1101 2 0.9625303370992846 1101 3 0.6405299927574788 1101 4 0.6030061679701433 1101 5 0.6954088872928139 1101 6 0.7421863629615117 1101 7 0.8166429210523631 1101 8 0.8921378855219189 1101 9 1.0937479481560217 1101 10-11 1.0072700301454987 1101 12-13 0.9684396757472413 1101 14-15 1.1947529150654361 1101 16-17 1.1452142529147054 1101 18-19 1.1964031149042782 1101 20-21 1.232199320011837 1101 22-23 1.2073559940493084 1101 24-25 1.2205037441551525 1101 26-27 1.0690857610470417 1101 28-29 1.2110535844325128 1101 30-31 1.256089128979312 1101 32-33 1.2978855983319804 1101 34-35 1.4633729767729804 1101 36-37 1.4234195165740076 1101 38-39 1.4255928588884572 1101 40-41 1.117020975781628 1101 42-43 1.1623765325640605 1101 44-45 1.1606588413198224 1101 46-47 1.218606571931197 1101 48-49 1.2794795584608423 1101 50-51 1.2519361889595189 1101 52-53 1.1629045576280888 1101 54-55 1.2895316058707422 1101 56-57 1.2403277570333664 1101 58-59 1.3549730922727719 1101 60-61 1.1919981797072907 1101 62-63 1.1737219249171815 1101 64-65 1.1435666004087501 1101 66-67 1.2231765203831841 1101 68-69 1.2817352224890612 1101 70-71 1.2326983254647104 1101 72-73 1.194372824377659 1101 74-75 1.2598498659858777 1101 76-77 1.226424247917766 1101 78-79 1.2436359008778979 1101 80-81 1.1409870768329284 1101 82-83 1.2766299296643169 1101 84-85 1.2329736541621585 1101 86-87 1.2675012697748897 1101 88-89 1.2859908854747495 1101 90-91 1.2033118499759752 1101 92-93 1.1897432817254412 1101 94-95 1.1492869720415584 1101 96-97 1.213985894100869 1101 98-99 1.1739987516903483 1101 100-101 1.1670826476717906 1101 102-103 1.101647605384379 1101 104-105 1.1921526888868677 1101 106-107 1.1873682020788117 1101 108-109 1.1781031850408894 1101 110-111 1.026599288901295 1101 112-113 1.0729238237875087 1101 114-115 1.1688620562547811 1101 116-117 1.129541770158248 1101 118-119 1.1489314883148651 1101 120-121 1.1701421745290084 1101 122-123 1.047838681777712 1101 124-125 1.4505879794361007 1102 1 0.5614017137810912 1102 2 -0.15668139207128107 1102 3 0.4512188275921538 1102 4 0.5017096241728609 1102 5 0.6946939906046765 1102 6 0.48223848666844304 1102 7 0.6100723936673731 1102 8 0.7364681627131233 1102 9 1.1426744670943023 1102 10-11 1.1508605516498704 1102 12-13 1.1995237152008826 1102 14-15 1.437229860348907 1102 16-17 1.4433241002857997 1102 18-19 1.5622890842715762 1102 20-21 1.3924984967245635 1102 22-23 1.5349159363122808 1102 24-25 1.4480373572636012 1102 26-27 1.4788000830378962 1102 28-29 1.090521722146022 1102 30-31 1.319647507531073 1102 32-33 1.4349237689235181 1102 34-35 1.510453648233245 1102 36-37 1.196951365495444 1102 38-39 0.7383386422142166 1102 40-41 0.3272340147273951 1102 42-43 0.7030099024285406 1102 44-45 0.2880135631930614 1102 46-47 0.5637618070224804 1102 48-49 0.31980326004593707 1102 50-51 0.19713305619108468 1102 52-53 -0.07824991565517081 1102 54-55 0.14798473462966655 1102 56-57 0.12937242304068874 1102 58-59 0.17736443439140004 1102 60-61 0.020474697309069256 1102 62-63 0.05329417639401868 1102 64-65 0.1276641920261845 1102 66-67 0.06184295538893281 1102 68-69 0.280889415106202 1102 70-71 -0.20981858132741138 1102 72-73 0.022609372451753984 1102 74-75 0.13291467312816252 1102 76-77 -0.11420217211912131 1102 78-79 0.05391061954352949 1102 80-81 0.03070213390170551 1102 82-83 -0.3551464195377889 1102 84-85 -0.2868701751928455 1102 86-87 -0.057867271563218026 1102 88-89 -0.1629502681966315 1102 90-91 -0.11488266500624889 1102 92-93 -0.05937121668411649 1102 94-95 0.20850371310702087 1102 96-97 -0.045513651487326 1102 98-99 -0.12683429206683527 1102 100-101 0.041663185481525034 1102 102-103 -0.048580937022936865 1102 104-105 0.0713663559901292 1102 106-107 0.21575343636819255 1102 108-109 0.22966328768449884 1102 110-111 0.3362117023625757 1102 112-113 0.5123900235683188 1102 114-115 0.43825020412572613 1102 116-117 0.35620458323461435 1102 118-119 0.5572342602268705 1102 120-121 0.8023978613590863 1102 122-123 0.7703449633526525 1102 124-125 1.0125122129503055 1103 1 0.33327064451771804 1103 2 -0.13289148917949234 1103 3 0.3155854781635341 1103 4 0.2176758777272383 1103 5 0.3521329626525329 1103 6 0.45044171645544395 1103 7 0.5569708324248168 1103 8 0.5702435798492331 1103 9 0.794084950489335 1103 10-11 0.7588526649606706 1103 12-13 0.769019664554385 1103 14-15 0.8280330150245874 1103 16-17 0.6887081069385204 1103 18-19 0.8615760631867531 1103 20-21 0.8610162551880052 1103 22-23 0.8950530144817108 1103 24-25 0.7916319707085222 1103 26-27 0.7965651187157761 1103 28-29 0.9165535639830296 1103 30-31 0.9475665214266655 1103 32-33 1.0069694795303121 1103 34-35 0.960737193487823 1103 36-37 0.9442406514013513 1103 38-39 1.0900344145196001 1103 40-41 0.9817503514407377 1103 42-43 0.871152506645501 1103 44-45 0.85208446291945 1103 46-47 1.0005195461715672 1103 48-49 0.8036234757227447 1103 50-51 0.7649081514215155 1103 52-53 0.8034454542681146 1103 54-55 0.760931512890302 1103 56-57 0.8018964318394417 1103 58-59 0.9303679485284277 1103 60-61 0.8923500661593309 1103 62-63 0.9120273434094202 1103 64-65 0.8173041941722232 1103 66-67 0.7245246979716882 1103 68-69 0.7446003169786195 1103 70-71 0.7559722336304731 1103 72-73 0.7223670042989596 1103 74-75 0.7656928532880407 1103 76-77 0.7904037616756092 1103 78-79 0.6587210975734692 1103 80-81 0.737573747185678 1103 82-83 0.7654067217251566 1103 84-85 0.7500080103321309 1103 86-87 0.7736828384212231 1103 88-89 0.7887725978369566 1103 90-91 0.7690113207225977 1103 92-93 0.7811246521929718 1103 94-95 0.7022725580021323 1103 96-97 0.71351244969836 1103 98-99 0.7868968630380522 1103 100-101 0.6612206992966456 1103 102-103 0.5433660558414886 1103 104-105 0.6030210853210995 1103 106-107 0.6785153871166223 1103 108-109 0.6726277707576251 1103 110-111 0.5343800590340706 1103 112-113 0.5249038386871554 1103 114-115 0.5871694057996351 1103 116-117 0.6760679074472868 1103 118-119 0.5477254346461038 1103 120-121 0.6314025021659937 1103 122-123 0.7120831199061186 1103 124-125 0.9415399236644006 1104 1 0.47872744271920453 1104 2 0.7047838858900732 1104 3 0.3355795032642561 1104 4 0.10640694633310943 1104 5 0.4476627447351049 1104 6 0.5525017767369036 1104 7 0.5651172005615521 1104 8 0.49131603075087327 1104 9 0.6433033496975966 1104 10-11 0.6252787228941514 1104 12-13 0.560085321376043 1104 14-15 0.7334339630214544 1104 16-17 0.6765073062917679 1104 18-19 0.7099802735707925 1104 20-21 0.6386351247083866 1104 22-23 0.7114814019603273 1104 24-25 0.777694762957637 1104 26-27 0.7611343371815451 1104 28-29 0.9621871772201729 1104 30-31 0.7603274752806826 1104 32-33 0.9759263066591188 1104 34-35 1.04583483726811 1104 36-37 0.9980788074051006 1104 38-39 1.219400327693613 1104 40-41 0.998420934815158 1104 42-43 0.9894299769745203 1104 44-45 0.8286508388281604 1104 46-47 0.7762774035225206 1104 48-49 0.9506499649548701 1104 50-51 0.8770045833263538 1104 52-53 0.9486506103962782 1104 54-55 0.588645074580505 1104 56-57 0.7613293872744755 1104 58-59 0.7906950141053741 1104 60-61 0.9307878168734263 1104 62-63 0.8924407967117673 1104 64-65 0.8792192159496288 1104 66-67 0.8274354902167076 1104 68-69 0.5976849291298372 1104 70-71 0.33897335078816226 1104 72-73 0.3257790967373566 1104 74-75 0.7832260083471745 1104 76-77 0.8793365151313992 1104 78-79 0.9389442577575977 1104 80-81 0.8643266019024445 1104 82-83 0.9023878795449249 1104 84-85 0.9388091316881173 1104 86-87 1.0317567602642157 1104 88-89 0.9240177325435397 1104 90-91 1.0055216544312202 1104 92-93 0.922997534802839 1104 94-95 0.886510202837119 1104 96-97 0.8989565301193743 1104 98-99 0.9085984447224007 1104 100-101 0.8991107125110531 1104 102-103 0.7630463859303518 1104 104-105 0.8521962087485946 1104 106-107 0.874702126526536 1104 108-109 0.8593538689845595 1104 110-111 0.8893854579469149 1104 112-113 0.8815795342611743 1104 114-115 0.8923629218819471 1104 116-117 0.9148073086569113 1104 118-119 0.9023904895747279 1104 120-121 0.8100868697917676 1104 122-123 0.8360322006573639 1104 124-125 1.111404531013168 1105 1 0.32435117458907214 1105 2 0.16960596240052794 1105 3 0.40809312342347326 1105 4 0.4834760816008341 1105 5 0.14214315633245178 1105 6 0.4642031843453509 1105 7 0.6596721576032039 1105 8 0.8133628459042797 1105 9 1.0007618108359182 1105 10-11 0.8254938474275448 1105 12-13 0.9661399499774248 1105 14-15 0.9987771536586365 1105 16-17 0.9989782394563562 1105 18-19 1.042896144736197 1105 20-21 0.9588755824051347 1105 22-23 1.0566228411891458 1105 24-25 0.9568970063863986 1105 26-27 1.0186599199390258 1105 28-29 0.9765688545029079 1105 30-31 1.0404564296835446 1105 32-33 1.1351550045048242 1105 34-35 1.1386169080647832 1105 36-37 1.2016310693422199 1105 38-39 1.1505593890354007 1105 40-41 0.9439063147434865 1105 42-43 0.8353472059319458 1105 44-45 1.0201527605749021 1105 46-47 0.9113248468851296 1105 48-49 0.9183023748053145 1105 50-51 0.7793067243063234 1105 52-53 0.9360907142069479 1105 54-55 0.8415890052450479 1105 56-57 0.8612746173644119 1105 58-59 0.5274627497516633 1105 60-61 0.576855672683287 1105 62-63 0.797221023327868 1105 64-65 0.6028546528878209 1105 66-67 0.7854319354844321 1105 68-69 0.7152934872130743 1105 70-71 0.7813290124276193 1105 72-73 0.9062355058280076 1105 74-75 0.7445409674572581 1105 76-77 0.8300316923585846 1105 78-79 0.7324978559832545 1105 80-81 0.7580885280215597 1105 82-83 0.7390305749361659 1105 84-85 0.7677195291904368 1105 86-87 0.8455482818462983 1105 88-89 0.786224177857342 1105 90-91 0.6028543380518556 1105 92-93 0.7334691985742126 1105 94-95 0.6535977363915322 1105 96-97 0.7087978727360742 1105 98-99 0.683940695861704 1105 100-101 0.7806141753414977 1105 102-103 0.41377889987818506 1105 104-105 0.582761146483179 1105 106-107 0.5868996888495488 1105 108-109 0.5978316443559919 1105 110-111 0.5030344932848365 1105 112-113 0.6133340119797133 1105 114-115 0.6475669593164568 1105 116-117 0.3394216281404603 1105 118-119 0.6455847618632262 1105 120-121 0.6510253360090132 1105 122-123 0.6741116622098886 1105 124-125 1.1277020031751057 1106 1 0.16652308809515048 1106 2 -0.14652298372375583 1106 3 0.39587484359518044 1106 4 0.12135698989069965 1106 5 0.38352525597920817 1106 6 0.6264503645912924 1106 7 0.7582390738501061 1106 8 0.7044318315153291 1106 9 0.8283833849976858 1106 10-11 0.7850279617004148 1106 12-13 0.8097511906692105 1106 14-15 0.8521674161027022 1106 16-17 0.7667836061382616 1106 18-19 0.9026013055026212 1106 20-21 0.9029935228105828 1106 22-23 0.925530782364234 1106 24-25 0.9762216083381219 1106 26-27 1.0107299751796575 1106 28-29 1.155451641319921 1106 30-31 1.0600498133727143 1106 32-33 0.9548044220200467 1106 34-35 0.9631513207658955 1106 36-37 1.0973298154724702 1106 38-39 1.0298708915586872 1106 40-41 1.0169350392455527 1106 42-43 0.8700481258762167 1106 44-45 0.8433901885005639 1106 46-47 0.8435050692612762 1106 48-49 1.0939118751893417 1106 50-51 1.0061677384282603 1106 52-53 0.9337466782950443 1106 54-55 0.8985275400252917 1106 56-57 0.4664118629347165 1106 58-59 1.0435851383083588 1106 60-61 0.9996868455533345 1106 62-63 0.8993411988036684 1106 64-65 1.0272674953538434 1106 66-67 0.874393978555478 1106 68-69 0.9815010848815646 1106 70-71 1.0738161603804812 1106 72-73 1.0095346691295042 1106 74-75 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2216 96-97 -2.6190882128908335 2216 98-99 -2.7039011068310153 2216 100-101 -2.733322289569738 2216 102-103 -2.570036564728568 2216 104-105 -2.618957464925799 2216 106-107 -2.606341289081797 2216 108-109 -2.6563993119965232 2216 110-111 -2.6486928886604346 2216 112-113 -2.608047148908323 2216 114-115 -2.713211119638231 2216 116-117 -2.72465210393786 2216 118-119 -2.725528897667864 2216 120-121 -2.82381853189678 2216 122-123 -2.8264108466268425 2216 124-125 -3.0231115054365674 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 82306.0 3 27844.0 4 13697.0 5 10892.0 6 11716.0 7 14000.0 8 15576.0 9 16575.0 10 18326.0 11 20041.0 12 22215.0 13 25429.0 14 29289.0 15 25339.0 16 17357.0 17 15137.0 18 14053.0 19 13524.0 20 13124.0 21 13316.0 22 15124.0 23 18886.0 24 24705.0 25 32110.0 26 40331.0 27 44840.0 28 50603.0 29 60331.0 30 70407.0 31 81896.0 32 99928.0 33 123592.0 34 159905.0 35 220837.0 36 349891.0 37 515815.0 38 188812.0 39 688.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.93722414214541 19.254972645234627 13.708702054637989 24.099101157981966 2 15.581326185041078 20.88485131967709 39.02238553209366 24.511436963188174 3 18.954105628962495 25.558228709086556 29.447356059682576 26.040309602268373 4 12.591315435110594 17.33252411363416 36.27780400985054 33.79835644140471 5 13.096590491717746 38.74304782650647 33.722116359342245 14.438245322433538 6 31.88349056585044 36.98026212081445 17.13739801791335 13.998849295421762 7 28.379486975857688 33.090891041931386 20.640168360864042 17.889453621346888 8 26.600939839622097 35.213886182038536 19.36670026073124 18.818473717608132 9 26.929459279269334 15.69913924175287 19.636541385448712 37.73486009352908 10-11 25.26161015421439 26.85604830287932 26.976062978619318 20.906278564286968 12-13 26.021488554301303 24.105632933180914 27.844390434301637 22.028488078216146 14-15 22.707936388815927 25.167915348150743 26.182524891734282 25.94162337129905 16-17 22.248689124002684 28.873856712235046 26.52495116538966 22.352502998372607 18-19 21.910441621313833 26.525828680250413 29.723967817687562 21.83976188074819 20-21 22.779972605845607 25.783601269128802 29.657716016844688 21.778710108180903 22-23 22.31679953241211 25.807647301502467 29.61333070209259 22.262222463992835 24-25 22.264128392900894 25.928912028277633 29.800806604996634 22.006152973824847 26-27 22.08004742586433 26.447284984417045 29.455198163002187 22.01746942671644 28-29 21.77331199222381 26.335788143295673 29.859413918919405 22.031485945561112 30-31 21.884570592231672 25.978843394983514 29.884667476951165 22.25191853583365 32-33 21.825625770064764 26.127386729255253 29.658358272545453 22.38862922813453 34-35 22.401533955116168 26.129054417049808 29.359623769633547 22.109787858200477 36-37 22.525081825895775 25.829902992189265 29.242746650032142 22.402268531882815 38-39 22.128013303383778 26.111781936320533 29.397662934090196 22.36254182620549 40-41 22.274948510139346 25.896392116283902 29.567906063117217 22.26075331045954 42-43 22.42041456336161 25.94336532249707 29.117828892849868 22.518391221291452 44-45 22.36502350447119 26.27674405399814 28.760923851389958 22.597308590140706 46-47 22.455197765933665 26.545023401233376 28.213624453385545 22.786154379447414 48-49 22.81988535043481 26.55393758956377 27.972564153368513 22.65361290663291 50-51 22.69162587943285 26.807223713105117 27.640293822940293 22.860856584521745 52-53 23.02505065601676 26.645144229184776 27.644903208591607 22.684901906206857 54-55 22.470385636919747 26.63088946922659 27.785743413526614 23.11298148032704 56-57 22.6455785776063 26.988812125187927 27.675754151077207 22.68985514612856 58-59 22.836664169085825 26.387973969569224 27.7799087291046 22.995453132240353 60-61 22.719149066273516 26.585464830251222 28.051660203052904 22.64372590042236 62-63 22.55859440919579 26.636507988820142 28.026327231316632 22.778570370667435 64-65 22.372221682870062 26.372930848236827 27.983065798117774 23.271781670775336 66-67 22.46369503231542 26.96589618166997 27.632951446064 22.937457339950612 68-69 22.555834782964332 26.6204267136584 27.881893556252894 22.94184494712437 70-71 22.342787667210516 26.746039340755072 27.88237003847991 23.028802953554496 72-73 22.535306340350463 26.68296500595404 27.81296246074481 22.968766192950685 74-75 22.519929567272328 26.873194684866352 27.501740666760245 23.105135081101075 76-77 22.606083804488225 26.778162184226296 27.656060834074196 22.959693177211285 78-79 22.600048362946044 26.867145240121232 27.55746871993447 22.975337676998254 80-81 22.36026756075945 27.21707268262777 27.568935093565262 22.85372466304752 82-83 22.6094787403557 26.997780784027682 27.401381083735 22.991359391881616 84-85 22.491886897413774 26.832044382731173 27.3053699149916 23.370698804863455 86-87 22.765169308032657 26.54956983581614 27.46654002827922 23.218720827871987 88-89 22.52694738697901 26.772277605139084 27.398303543562523 23.302471464319378 90-91 22.3081831454736 27.013921619467794 27.60334998771073 23.07454524734788 92-93 22.462126867449594 26.688149815280127 27.625388737646954 23.224334579623317 94-95 22.4307184915208 26.60378954256515 27.490562673891194 23.474929292022853 96-97 22.64067551887388 26.55086365805987 27.41362823747678 23.39483258558947 98-99 22.291389865936456 26.59018296842625 27.31444457062896 23.803982595008335 100-101 22.07008856219992 26.577058167300304 27.710833805359314 23.64201946514046 102-103 22.63737280406217 26.254905285259984 27.512897778282497 23.59482413239535 104-105 22.29192318947673 26.268921804104657 27.692908792963312 23.7462462134553 106-107 22.311947559319282 26.43658649557571 27.612856678564967 23.63860926654004 108-109 22.400978059184652 26.495588370180627 27.154066954488403 23.949366616146314 110-111 22.104592738243383 26.481285741172954 27.626968767346956 23.787152753236708 112-113 22.302524919027803 26.33721758997672 27.564516686209057 23.795740804786423 114-115 21.77682604864804 26.58332066022966 27.42077788105971 24.21907541006259 116-117 21.859118499938653 26.533945189455288 27.509205041023133 24.097731269582923 118-119 21.97198522746269 26.693566735505115 27.08499688499744 24.249451152034755 120-121 21.733453169636466 26.71829395670447 27.365739563312225 24.182513310346838 122-123 21.84485603963258 26.57231056111744 27.310715981848304 24.272117417401677 124-125 21.728600285251375 26.57145570794837 27.188394266836806 24.51154973996345 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 435.0 1 364.0 2 567.0 3 831.0 4 575.5 5 432.5 6 862.5 7 2092.5 8 3511.0 9 4023.0 10 3090.5 11 1931.0 12 1658.0 13 1671.5 14 1861.5 15 2102.0 16 2400.5 17 2778.0 18 3222.0 19 3659.0 20 4195.5 21 4946.5 22 5979.0 23 6977.0 24 8139.0 25 10184.0 26 13452.5 27 17137.0 28 20434.5 29 24432.0 30 28584.5 31 33214.5 32 38957.0 33 43542.5 34 47865.5 35 51766.5 36 56199.5 37 62391.0 38 65543.0 39 66101.0 40 67994.5 41 73873.0 42 81378.0 43 86343.5 44 89076.0 45 91954.0 46 92065.0 47 91312.0 48 97746.0 49 100542.5 50 96787.5 51 93520.5 52 89619.5 53 88666.0 54 87228.0 55 80028.5 56 70289.0 57 61789.5 58 56198.5 59 50578.0 60 44170.5 61 37046.0 62 30815.5 63 24925.5 64 19505.0 65 15667.5 66 11799.5 67 9689.0 68 7224.5 69 4591.0 70 3622.5 71 3092.5 72 2672.0 73 2449.5 74 2024.5 75 1646.0 76 1122.5 77 587.0 78 347.5 79 188.5 80 116.5 81 87.0 82 65.5 83 48.5 84 25.0 85 12.0 86 10.0 87 7.0 88 4.0 89 4.0 90 3.0 91 2.0 92 1.5 93 1.0 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09696413319743002 2 0.0 3 0.0 4 0.09740090857219322 5 0.0 6 0.0 7 0.002263290578318391 8 0.0061545620989359754 9 0.003811857816115185 10-11 5.956027837679976E-4 12-13 0.0 14-15 1.9853426125599922E-5 16-17 2.978013918839988E-4 18-19 0.0021838768738159913 20-21 0.0014493001071687942 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 5.956027837679977E-5 52-53 0.0 54-55 0.0 56-57 0.007742836188983969 58-59 7.941370450239968E-4 60-61 0.0 62-63 0.0 64-65 1.3897398287919944E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0010125247324055961 76-77 0.0 78-79 0.0 80-81 3.9706852251199845E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.001230912419787195 90-91 0.0 92-93 0.0019456357603087923 94-95 0.0 96-97 0.008536973234007966 98-99 0.003930978372868784 100-101 0.0022831440044439907 102-103 0.0 104-105 0.0 106-107 0.0012110589936615952 108-109 0.0 110-111 1.1912055675359953E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 8.536973234007965E-4 122-123 0.004963356531399981 124-125 0.08259025268249567 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2518457.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.00644570814317 #Duplication Level Percentage of deduplicated Percentage of total 1 73.13550780806256 33.6470476930911 2 12.740905592038398 11.723275627853845 3 5.34953910357799 7.383398409970489 4 2.7655436932857955 5.089313431146028 5 1.5775305617591704 3.6288287071254928 6 1.0165075987614367 2.8059540992599814 7 0.678972450979736 2.186597644232689 8 0.46417899871578044 1.7084180722622249 9 0.3550403674902539 1.4700730852025607 >10 1.6809041097178825 13.727128333436406 >50 0.13270337873934498 4.277648926209064 >100 0.0939082711916076 8.251950470010353 >500 0.0071105087699024835 2.249891159101524 >1k 0.0013874163453465574 0.9809488751376718 >5k 2.601405647524795E-4 0.8695254659604169 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8852 0.351485056127621 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7702 0.3058221760387412 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5242 0.20814331950078957 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.9706852251199845E-5 3.9706852251199845E-5 2 0.0 0.0 0.0 3.9706852251199845E-5 3.9706852251199845E-5 3 3.9706852251199845E-5 0.0 0.0 3.9706852251199845E-5 3.9706852251199845E-5 4 3.9706852251199845E-5 0.0 0.0 3.9706852251199845E-5 3.9706852251199845E-5 5 3.9706852251199845E-5 0.0 0.0 3.9706852251199845E-5 3.9706852251199845E-5 6 3.9706852251199845E-5 0.0 0.0 3.9706852251199845E-5 7.941370450239969E-5 7 3.9706852251199845E-5 0.0 0.0 3.9706852251199845E-5 7.941370450239969E-5 8 3.9706852251199845E-5 0.0 0.0 3.9706852251199845E-5 7.941370450239969E-5 9 3.9706852251199845E-5 0.0 0.0 1.1912055675359952E-4 7.941370450239969E-5 10-11 3.9706852251199845E-5 0.0 0.0 1.5882740900479938E-4 7.941370450239969E-5 12-13 3.9706852251199845E-5 0.0 0.0 1.5882740900479938E-4 7.941370450239969E-5 14-15 3.9706852251199845E-5 0.0 0.0 1.5882740900479938E-4 1.1912055675359953E-4 16-17 3.9706852251199845E-5 0.0 0.0 2.1838768738159913E-4 2.1838768738159913E-4 18-19 3.9706852251199845E-5 0.0 0.0 3.5736167026079863E-4 2.779479657583989E-4 20-21 3.9706852251199845E-5 0.0 0.0 5.558959315167978E-4 2.779479657583989E-4 22-23 3.9706852251199845E-5 0.0 0.0 8.735507495263965E-4 3.1765481800959876E-4 24-25 3.9706852251199845E-5 0.0 0.0 0.0012110589936615954 3.9706852251199845E-4 26-27 3.9706852251199845E-5 0.0 0.0 0.0014890069594199942 4.3677537476319826E-4 28-29 3.9706852251199845E-5 0.0 0.0 0.002243437152192791 4.3677537476319826E-4 30-31 3.9706852251199845E-5 0.0 0.0 0.005281011349409579 4.3677537476319826E-4 32-33 3.9706852251199845E-5 0.0 0.0 0.010502462420442359 4.3677537476319826E-4 34-35 3.9706852251199845E-5 0.0 0.0 0.016498197110373536 4.3677537476319826E-4 36-37 3.9706852251199845E-5 0.0 0.0 0.02717934036594629 4.3677537476319826E-4 38-39 3.9706852251199845E-5 0.0 0.0 0.04582170749788462 4.3677537476319826E-4 40-41 3.9706852251199845E-5 0.0 0.0 0.06831563929818932 4.3677537476319826E-4 42-43 3.9706852251199845E-5 0.0 0.0 0.09025367516697724 4.3677537476319826E-4 44-45 3.9706852251199845E-5 0.0 0.0 0.11681755932302994 4.3677537476319826E-4 46-47 3.9706852251199845E-5 0.0 0.0 0.1518390030085882 4.3677537476319826E-4 48-49 3.9706852251199845E-5 0.0 0.0 0.19069215793638725 4.3677537476319826E-4 50-51 3.9706852251199845E-5 0.0 0.0 0.23631533117301584 4.3677537476319826E-4 52-53 3.9706852251199845E-5 0.0 0.0 0.27727294927012847 4.96335653139998E-4 54-55 3.9706852251199845E-5 0.0 0.0 0.32176447721759793 5.558959315167978E-4 56-57 3.9706852251199845E-5 0.0 0.0 0.3758452099837321 5.558959315167978E-4 58-59 3.9706852251199845E-5 0.0 0.0 0.43999162979554546 5.558959315167978E-4 60-61 3.9706852251199845E-5 0.0 0.0 0.51063011995043 5.558959315167978E-4 62-63 3.9706852251199845E-5 0.0 0.0 0.5827179102124833 5.558959315167978E-4 64-65 3.9706852251199845E-5 0.0 0.0 0.6540711237078893 5.558959315167978E-4 66-67 3.9706852251199845E-5 0.0 0.0 0.7410688369902683 5.757493576423977E-4 68-69 3.9706852251199845E-5 0.0 0.0 0.8417257074470599 5.956027837679976E-4 70-71 3.9706852251199845E-5 0.0 0.0 0.9616602546718089 7.147233405215971E-4 72-73 3.9706852251199845E-5 0.0 0.0 1.08248820607221 8.735507495263965E-4 74-75 3.9706852251199845E-5 0.0 0.0 1.2053412069374225 8.735507495263965E-4 76-77 3.9706852251199845E-5 0.0 0.0 1.3439379747202354 8.735507495263965E-4 78-79 7.941370450239969E-5 0.0 0.0 1.503261719378175 8.735507495263965E-4 80-81 7.941370450239969E-5 0.0 0.0 1.6874221001192398 8.735507495263965E-4 82-83 7.941370450239969E-5 0.0 0.0 1.8840901393194325 8.735507495263965E-4 84-85 7.941370450239969E-5 0.0 0.0 2.0780184057142925 8.735507495263965E-4 86-87 7.941370450239969E-5 0.0 0.0 2.2838785812106384 8.735507495263965E-4 88-89 7.941370450239969E-5 0.0 0.0 2.5179306218053354 8.735507495263965E-4 90-91 7.941370450239969E-5 0.0 0.0 2.7900218268566825 8.735507495263965E-4 92-93 7.941370450239969E-5 0.0 0.0 3.0761096973265776 8.735507495263965E-4 94-95 7.941370450239969E-5 0.0 0.0 3.370198498525089 9.132576017775964E-4 96-97 7.941370450239969E-5 0.0 0.0 3.6761596485467094 9.132576017775964E-4 98-99 7.941370450239969E-5 0.0 0.0 4.00878394985501 9.132576017775964E-4 100-101 7.941370450239969E-5 0.0 0.0 4.392769064550238 9.132576017775964E-4 102-103 1.1912055675359952E-4 0.0 0.0 4.8051644320312 9.132576017775964E-4 104-105 1.1912055675359952E-4 0.0 0.0 5.216368593944626 9.132576017775964E-4 106-107 1.1912055675359952E-4 0.0 0.0 5.635950901683055 9.331110279031963E-4 108-109 1.1912055675359952E-4 0.0 0.0 6.076835935654252 0.0010323781585311958 110-111 1.5882740900479938E-4 0.0 0.0 6.565269925196261 0.001052231584656796 112-113 1.5882740900479938E-4 0.0 0.0 7.106514028232366 0.0010720850107823958 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6030 0.0 58.622616 1 GTATCAA 10430 0.0 47.38032 1 ATCAACG 13605 0.0 36.59421 3 TCAACGC 13625 0.0 36.45308 4 CAACGCA 13825 0.0 35.80613 5 AACGCAG 14070 0.0 35.22493 6 TATCAAC 13950 0.0 35.091526 2 GTACATG 12405 0.0 34.166508 1 TACATGG 12785 0.0 32.699493 2 ACATGGG 13135 0.0 30.558674 3 ACGCAGA 16205 0.0 30.216906 7 CGCAGAG 16410 0.0 29.694399 8 GCAGAGT 17870 0.0 26.801674 9 GTGGTAT 3230 0.0 26.391333 1 TGGTATC 3290 0.0 25.884798 2 GAGTACT 10500 0.0 25.611778 12-13 CATGGGG 5635 0.0 25.364248 4 AGTACTT 10755 0.0 24.700266 12-13 CATGGGA 8070 0.0 24.204994 4 GTACTTT 11415 0.0 23.741222 14-15 >>END_MODULE