FastQCFastQC Report
Thu 26 May 2016
SRR1364585_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364585_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2872682
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32050.11156821395476424No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA72350.048.1897431
GGTATCA42600.046.6479341
ATCAACG82700.040.5745773
TCAACGC84450.039.663334
CAACGCA84850.039.406235
TATCAAC88400.038.6986662
AACGCAG86500.038.5109566
GTACATG106650.034.1975631
ACGCAGA100450.032.689567
TACATGG110200.031.6370852
CGCAGAG103300.030.6967378
ACATGGG115000.028.919743
GCAGAGT112000.027.1986569
CATGGGG61600.024.5320264
GAGTACT49600.023.45708512-13
AGAGTAC98850.022.93589410-11
CAGAGTA109950.022.16287210-11
GAGTACA86250.021.7293621
GTACTTT54550.021.27398314-15
AGTACAT80400.021.237642
Produced by FastQC (version 0.11.5)