##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364585_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2872682 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.668723513427523 31.0 28.0 33.0 12.0 34.0 2 27.580042622190692 31.0 27.0 33.0 10.0 34.0 3 27.831222181919195 31.0 28.0 34.0 15.0 34.0 4 31.116471993767497 35.0 32.0 37.0 16.0 37.0 5 31.236101663880653 35.0 32.0 37.0 16.0 37.0 6 31.155936856220077 35.0 32.0 37.0 13.0 37.0 7 30.972067218021348 35.0 32.0 37.0 10.0 37.0 8 30.940659286339386 35.0 32.0 37.0 10.0 37.0 9 32.0092453672213 37.0 32.0 39.0 10.0 39.0 10-11 31.91256428661439 37.0 31.5 39.0 9.0 39.0 12-13 31.823909155277192 37.0 31.0 39.0 8.0 39.0 14-15 32.47603841984598 38.0 31.0 40.0 8.0 41.0 16-17 32.55321681968279 38.0 31.0 40.0 4.5 41.0 18-19 32.33215197505328 38.0 31.0 40.0 2.0 41.0 20-21 32.03823273860455 38.0 30.0 40.0 2.0 41.0 22-23 31.907995907657025 38.0 30.0 40.0 2.0 41.0 24-25 31.721142820541917 38.0 30.0 40.0 2.0 41.0 26-27 31.601687725964794 37.5 29.5 40.0 2.0 41.0 28-29 31.636208254168054 37.5 30.0 40.0 2.0 41.0 30-31 31.578141959325816 37.5 30.0 40.0 2.0 41.0 32-33 31.5669630331516 37.0 30.0 40.0 2.0 41.0 34-35 31.097860988442157 37.0 29.0 40.0 2.0 41.0 36-37 30.947675726028848 37.0 28.5 40.0 2.0 41.0 38-39 30.74458572163574 37.0 28.0 40.0 2.0 41.0 40-41 30.940188471957562 37.0 29.0 40.0 2.0 41.0 42-43 30.810987954810173 37.0 29.0 40.0 2.0 41.0 44-45 30.65110287181108 37.0 28.5 40.0 2.0 41.0 46-47 30.60433264106504 37.0 28.5 40.0 2.0 41.0 48-49 30.460308694105372 36.5 28.0 40.0 2.0 41.0 50-51 30.29217452540866 36.0 28.0 40.0 2.0 41.0 52-53 30.22795648804845 36.0 28.0 40.0 2.0 41.0 54-55 30.24403571296788 36.0 28.0 40.0 2.0 41.0 56-57 30.09995954999544 36.0 28.0 40.0 2.0 41.0 58-59 29.882391611741223 35.5 28.0 40.0 2.0 41.0 60-61 29.66234759016139 35.0 28.0 39.0 2.0 41.0 62-63 29.368756792432997 35.0 27.0 39.0 2.0 41.0 64-65 29.072862398274502 35.0 26.5 39.0 2.0 40.5 66-67 28.823443388443273 34.5 26.0 38.5 2.0 40.0 68-69 28.795745230415342 35.0 27.0 38.0 2.0 40.0 70-71 28.565315095788534 34.0 26.0 37.0 2.0 40.0 72-73 28.29345503609519 34.0 26.0 37.0 2.0 39.5 74-75 28.00015856262545 34.0 26.0 36.5 2.0 39.0 76-77 27.70818123969169 34.0 26.0 36.0 2.0 39.0 78-79 27.420262145270517 34.0 26.0 35.5 2.0 37.5 80-81 27.14006109969708 34.0 26.0 35.0 2.0 37.0 82-83 26.915795587538057 34.0 25.0 35.0 2.0 37.0 84-85 26.682069056024996 33.5 25.0 35.0 2.0 36.0 86-87 26.50448535549706 33.0 25.0 35.0 2.0 36.0 88-89 26.3282648410092 33.0 24.0 35.0 2.0 36.0 90-91 26.203074165535902 33.0 24.0 35.0 2.0 35.0 92-93 26.082751414879894 33.0 24.0 35.0 2.0 35.0 94-95 25.960480136680637 33.0 23.0 35.0 2.0 35.0 96-97 25.85380473717592 33.0 23.0 35.0 2.0 35.0 98-99 25.767001185651594 33.0 23.0 35.0 2.0 35.0 100-101 25.67581357769499 33.0 21.5 35.0 2.0 35.0 102-103 24.546228924746977 31.0 19.0 34.0 2.0 35.0 104-105 25.39956789508898 33.0 20.0 35.0 2.0 35.0 106-107 25.541293119113078 33.0 20.0 35.0 2.0 35.0 108-109 25.49409523922244 33.0 19.5 35.0 2.0 35.0 110-111 25.41274826103272 33.0 19.0 35.0 2.0 35.0 112-113 25.321233084622662 33.0 18.5 35.0 2.0 35.0 114-115 25.191530075379035 33.0 18.0 35.0 2.0 35.0 116-117 25.080039837336678 33.0 16.0 35.0 2.0 35.0 118-119 24.92699244120999 33.0 8.5 35.0 2.0 35.0 120-121 24.740336904676536 33.0 2.0 35.0 2.0 35.0 122-123 24.49123850116372 33.0 2.0 35.0 2.0 35.0 124-125 23.045226899461895 31.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6253103839044449 1101 2 0.6929085874490468 1101 3 0.685225482847116 1101 4 0.7909666854488293 1101 5 0.6576211449957796 1101 6 0.6537208573685334 1101 7 0.8602983264485289 1101 8 0.8523550875180632 1101 9 1.0702118643175567 1101 10-11 1.0597879572052378 1101 12-13 0.837694438093866 1101 14-15 1.161029618626145 1101 16-17 1.0360091893681513 1101 18-19 1.4158478463178028 1101 20-21 1.7471511424707415 1101 22-23 1.644372563298596 1101 24-25 1.7850293397491548 1101 26-27 1.7685823713044968 1101 28-29 1.5792195063349581 1101 30-31 1.71745857527862 1101 32-33 1.759515173657924 1101 34-35 2.0010222039807495 1101 36-37 1.8782686230880756 1101 38-39 1.703866040215221 1101 40-41 1.6139599561406612 1101 42-43 1.5545154146650297 1101 44-45 1.579518544133915 1101 46-47 1.3527829761038106 1101 48-49 1.3708130855466045 1101 50-51 1.4674986724376637 1101 52-53 1.2957538290481807 1101 54-55 1.188028488575263 1101 56-57 1.1994634447684192 1101 58-59 1.213502110039105 1101 60-61 1.2070057095661717 1101 62-63 1.174301439082889 1101 64-65 1.2182716819101316 1101 66-67 1.2076895385756146 1101 68-69 1.0357929780150066 1101 70-71 0.9614414897082355 1101 72-73 0.8469328897946085 1101 74-75 0.8312535692390135 1101 76-77 1.0167778941863759 1101 78-79 0.9626378268222986 1101 80-81 1.1049490991117779 1101 82-83 0.9383737744161174 1101 84-85 1.0840692134908387 1101 86-87 1.0885642445239654 1101 88-89 1.034449341468438 1101 90-91 1.06383253211823 1101 92-93 0.9333944633834577 1101 94-95 0.9432575424391594 1101 96-97 0.8981287471091655 1101 98-99 0.9850812421988167 1101 100-101 1.109042999475399 1101 102-103 1.0203529067336312 1101 104-105 1.0901221110569566 1101 106-107 0.9850833036271602 1101 108-109 1.0684166030742297 1101 110-111 0.8479005223189766 1101 112-113 0.9256102151576897 1101 114-115 1.1839249960434124 1101 116-117 1.1688446519147035 1101 118-119 1.198420085164969 1101 120-121 1.2478723138872247 1101 122-123 1.3413479916066535 1101 124-125 1.4464688880682672 1102 1 -0.4000719852517456 1102 2 -0.15246605980990324 1102 3 -0.0017791510222693319 1102 4 0.16701765038834537 1102 5 0.23301272006543527 1102 6 -0.714402526309609 1102 7 -1.3429383223399718 1102 8 -0.40143685591345246 1102 9 0.05535499251305964 1102 10-11 -0.04076380232523036 1102 12-13 0.13887524400971074 1102 14-15 0.4035204547476283 1102 16-17 0.12220894024975593 1102 18-19 0.3770726223498855 1102 20-21 0.6037611815428718 1102 22-23 0.517130442970462 1102 24-25 0.6200703883063561 1102 26-27 0.15477435747066437 1102 28-29 0.6074393124269832 1102 30-31 0.5124523076337368 1102 32-33 0.38693666047926456 1102 34-35 0.7175706663007837 1102 36-37 0.801287030633187 1102 38-39 0.8394484413910739 1102 40-41 0.5824528086861598 1102 42-43 0.4327768953999147 1102 44-45 0.42665179239479656 1102 46-47 0.14186683053988958 1102 48-49 -0.1901265108156025 1102 50-51 0.209732468802855 1102 52-53 0.06435909503954917 1102 54-55 -0.1657740340852243 1102 56-57 -0.024347271833875084 1102 58-59 0.14100494100109273 1102 60-61 0.0553328285162884 1102 62-63 -0.06808055003564917 1102 64-65 -0.0050154255187564445 1102 66-67 0.01719704883605644 1102 68-69 -0.054093106769002475 1102 70-71 -0.13200469360776523 1102 72-73 -0.022811136726776482 1102 74-75 -0.29525064203560447 1102 76-77 -0.013411366444572081 1102 78-79 -0.15687174393357495 1102 80-81 -0.46809992866961636 1102 82-83 -0.35956146399302114 1102 84-85 -0.5750262029041693 1102 86-87 -0.12770561228674637 1102 88-89 0.07906413273440194 1102 90-91 -0.3481324087555713 1102 92-93 0.0329038607778358 1102 94-95 -0.08920685292030583 1102 96-97 0.03892230624898829 1102 98-99 0.17890710286818035 1102 100-101 0.17090049799216445 1102 102-103 0.1002337614308999 1102 104-105 0.30121451618356687 1102 106-107 0.145320843347573 1102 108-109 -0.3686796721912522 1102 110-111 -0.09921844972669192 1102 112-113 -0.34822250060140547 1102 114-115 -0.3499596059775847 1102 116-117 -0.4651899495788996 1102 118-119 -0.09947596414572502 1102 120-121 0.2506588650450041 1102 122-123 0.18544775300483707 1102 124-125 0.40729681247054117 1103 1 0.5926828904840562 1103 2 0.5410021984762636 1103 3 0.38170143671074896 1103 4 0.4029077382183388 1103 5 0.550384992537392 1103 6 0.5803304448226108 1103 7 0.6651781437055604 1103 8 0.6494635148515293 1103 9 0.9207000218191084 1103 10-11 0.967631225469546 1103 12-13 1.013347794933221 1103 14-15 1.3107401078103393 1103 16-17 1.1059627845435784 1103 18-19 1.2438536732872478 1103 20-21 1.5580661136994323 1103 22-23 1.4388493964409719 1103 24-25 1.5148746410209775 1103 26-27 1.4694394883088933 1103 28-29 1.4624206482387976 1103 30-31 1.4791787796739797 1103 32-33 1.4162908033749133 1103 34-35 1.8143339188819105 1103 36-37 1.796466786789722 1103 38-39 1.729183405510998 1103 40-41 1.3631463935956276 1103 42-43 1.3158937550054226 1103 44-45 1.0484508117063136 1103 46-47 1.0801014495864294 1103 48-49 1.0232632810572895 1103 50-51 1.0296039258185523 1103 52-53 0.9960281891841376 1103 54-55 1.0110778859836884 1103 56-57 0.8227834870505042 1103 58-59 0.8605073489584356 1103 60-61 0.762586233282331 1103 62-63 0.6642186965961088 1103 64-65 0.841595519352623 1103 66-67 0.9517400753311449 1103 68-69 0.909455683333185 1103 70-71 0.9570326618455276 1103 72-73 0.9916129937260756 1103 74-75 0.9351063861269182 1103 76-77 0.9781650333065706 1103 78-79 0.9765374520202492 1103 80-81 0.9157327378157021 1103 82-83 1.0351965060687114 1103 84-85 0.9703396913774363 1103 86-87 0.8666897788425985 1103 88-89 0.9254561795485152 1103 90-91 0.9287096408292044 1103 92-93 1.0374008344590635 1103 94-95 0.9553233561135634 1103 96-97 0.988906737016432 1103 98-99 0.9518876207049516 1103 100-101 0.9843780222543472 1103 102-103 0.8425131380067121 1103 104-105 0.8748251077955125 1103 106-107 0.9471837830957419 1103 108-109 1.0530657285094769 1103 110-111 1.087887990299805 1103 112-113 1.1451387723415678 1103 114-115 1.2229702055921976 1103 116-117 1.2195965331157943 1103 118-119 1.23099579462027 1103 120-121 1.2491686108493383 1103 122-123 1.2885922657083206 1103 124-125 1.2890823373976872 1104 1 0.41332094592738144 1104 2 0.4656174608669019 1104 3 0.32119497233921734 1104 4 0.47296648697496124 1104 5 0.4668902566528068 1104 6 0.44203873060642707 1104 7 0.5636309828547041 1104 8 0.5712981642137152 1104 9 0.9039482680984321 1104 10-11 0.8389843104585566 1104 12-13 0.7619510648041974 1104 14-15 0.7966492073110985 1104 16-17 0.7951307093527333 1104 18-19 1.0418823766562326 1104 20-21 1.397781492926022 1104 22-23 1.1552303377678257 1104 24-25 1.5923053368623705 1104 26-27 1.533027737258081 1104 28-29 1.3942886885844175 1104 30-31 1.2723542574727844 1104 32-33 1.2479341353378715 1104 34-35 1.588744910321818 1104 36-37 1.5958743523017347 1104 38-39 1.5618983736262386 1104 40-41 1.2208501831444138 1104 42-43 1.2646886110337583 1104 44-45 1.1418785972810284 1104 46-47 1.077506530710039 1104 48-49 0.9905075709805757 1104 50-51 0.7835934336190356 1104 52-53 0.7976104805628452 1104 54-55 0.6939960765975925 1104 56-57 0.7351294974984022 1104 58-59 0.6492101449881389 1104 60-61 0.6280196249745735 1104 62-63 0.722109199215172 1104 64-65 0.7688635131528727 1104 66-67 0.6652193147987795 1104 68-69 0.6703241442409862 1104 70-71 0.6466931376242187 1104 72-73 0.6930318594840479 1104 74-75 0.6915147171566112 1104 76-77 0.7309806439604998 1104 78-79 0.7729530897271921 1104 80-81 0.7594707766019297 1104 82-83 0.7056119057327344 1104 84-85 0.6362181731243304 1104 86-87 0.783452430558043 1104 88-89 0.7323175009407201 1104 90-91 0.8267044097917129 1104 92-93 0.796976007586558 1104 94-95 0.7688601423096699 1104 96-97 0.6540201440899125 1104 98-99 0.7119091243092619 1104 100-101 0.687884710492547 1104 102-103 0.7289233237628885 1104 104-105 0.7356403634524291 1104 106-107 0.7466933880980697 1104 108-109 0.8757437615779509 1104 110-111 0.895000423346545 1104 112-113 0.9092011726647868 1104 114-115 0.9110143669841158 1104 116-117 0.9475929205338964 1104 118-119 1.001074168627639 1104 120-121 0.911601229345095 1104 122-123 0.8666930848594916 1104 124-125 1.1969011761026351 1105 1 -0.16325421239282178 1105 2 0.19927981989771837 1105 3 0.3657236253482701 1105 4 0.3239580658087675 1105 5 0.31572481425927634 1105 6 0.29727045584732537 1105 7 0.34110802507418825 1105 8 0.12155116892433071 1105 9 0.11550399606179695 1105 10-11 0.22822384285844066 1105 12-13 0.2636922923872511 1105 14-15 0.4766554065586135 1105 16-17 0.3796405963846965 1105 18-19 0.45932430382603684 1105 20-21 0.840063642511673 1105 22-23 1.1335040194826291 1105 24-25 1.1622807614137507 1105 26-27 0.8762864443863307 1105 28-29 0.7145485102176465 1105 30-31 0.6514646762139549 1105 32-33 0.6792041355036602 1105 34-35 1.3643260861081892 1105 36-37 1.3908806031444385 1105 38-39 1.4282846607890214 1105 40-41 0.8892915042296856 1105 42-43 0.6338302035390626 1105 44-45 0.8664781024660506 1105 46-47 0.7366553871332435 1105 48-49 0.5995247814238525 1105 50-51 0.614210072416963 1105 52-53 0.5540073425385756 1105 54-55 0.35650118782239204 1105 56-57 0.4140800738557857 1105 58-59 0.3334197626932216 1105 60-61 0.3990642632245631 1105 62-63 0.3263568134756518 1105 64-65 0.5100424842450444 1105 66-67 0.2988759176649367 1105 68-69 0.16134472299424374 1105 70-71 0.3078112674741966 1105 72-73 0.3711780399509088 1105 74-75 0.35519795927894293 1105 76-77 0.41547965932378617 1105 78-79 0.4885181306465789 1105 80-81 0.44532334650448746 1105 82-83 0.45596662876853955 1105 84-85 0.4891773626015734 1105 86-87 0.46734166903898 1105 88-89 0.5982083031226608 1105 90-91 0.45525272218155877 1105 92-93 0.42536185626359213 1105 94-95 0.5386682566238754 1105 96-97 0.502663775045324 1105 98-99 0.325532391303895 1105 100-101 0.32436310558189874 1105 102-103 0.27407725666361316 1105 104-105 0.4160796688530546 1105 106-107 0.3188781381937176 1105 108-109 0.2828682159710567 1105 110-111 0.41225691105028517 1105 112-113 0.4732972398740962 1105 114-115 0.46522029673357324 1105 116-117 0.47893413673177676 1105 118-119 0.47864967696373384 1105 120-121 0.33146187049576525 1105 122-123 0.414041989571885 1105 124-125 0.5421785266510604 1106 1 0.1949451300902787 1106 2 0.39135390708915807 1106 3 0.14963418113663707 1106 4 0.19660823532263194 1106 5 0.16782283008488363 1106 6 0.2083982814911316 1106 7 -0.13333713565923944 1106 8 0.2084977017588372 1106 9 0.22095797402287332 1106 10-11 0.3728423360923472 1106 12-13 0.31558189540884385 1106 14-15 0.7073431470806 1106 16-17 0.47211452343198346 1106 18-19 0.47986895265205476 1106 20-21 0.9012032788376771 1106 22-23 0.8601748898829058 1106 24-25 0.9746546064483006 1106 26-27 1.2089294314690662 1106 28-29 1.1009457427276814 1106 30-31 0.8180608931973623 1106 32-33 0.7091932112543518 1106 34-35 1.1195314787736983 1106 36-37 0.9692871333954471 1106 38-39 1.1456996102320254 1106 40-41 0.876584812237521 1106 42-43 0.7826966004147771 1106 44-45 0.5991155910369308 1106 46-47 0.42821515120665765 1106 48-49 0.5372742486039002 1106 50-51 0.4745119639656892 1106 52-53 0.347287857813825 1106 54-55 0.2304340065192747 1106 56-57 0.2282085405209564 1106 58-59 0.2373025156202182 1106 60-61 0.32081897380169977 1106 62-63 0.2717565653957337 1106 64-65 0.22185771989367709 1106 66-67 0.2258570193605216 1106 68-69 0.25015890523312123 1106 70-71 0.2947867685715764 1106 72-73 0.32740377043932867 1106 74-75 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-2.212659689552247 2216 122-123 -2.1997789962732845 2216 124-125 -2.2901340543798483 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 231941.0 3 37318.0 4 23211.0 5 18596.0 6 17346.0 7 17932.0 8 18740.0 9 19510.0 10 19860.0 11 20738.0 12 22165.0 13 22995.0 14 24273.0 15 21367.0 16 17579.0 17 15694.0 18 14979.0 19 14149.0 20 14175.0 21 15332.0 22 18419.0 23 24099.0 24 31502.0 25 40031.0 26 48503.0 27 56305.0 28 64654.0 29 75452.0 30 85784.0 31 98420.0 32 119774.0 33 149328.0 34 191774.0 35 261653.0 36 395271.0 37 470226.0 38 133361.0 39 226.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.780558963568076 18.831771991661086 13.290111533689188 24.09755751108165 2 15.082094820875799 20.04733106541546 40.05762163833162 24.812952475377124 3 18.886123525076442 25.115781477539805 28.61333425241354 27.38476074497021 4 12.360235883985297 16.45349632662809 37.107340164021466 34.078927625365154 5 14.004148655273069 37.7071194211101 33.902374997214736 14.386356926402103 6 32.18409210453487 36.39097571614214 17.336924907985626 14.08800727133736 7 28.866482201990213 31.900192727060052 21.079974767237513 18.153350303712223 8 26.21950497827466 35.42396965623066 19.66051237136585 18.696012994128832 9 26.564707823617447 16.40188303475914 19.643026849920126 37.39038229170329 10-11 25.06462601847333 26.692582053983006 27.234166538447347 21.008625389096323 12-13 25.78363176813241 23.896785126770606 27.463573130755194 22.856009974341795 14-15 22.902109680401306 25.311098866950953 25.040061905545297 26.746729547102444 16-17 23.01357021888792 28.681666841646074 25.39057405955533 22.914188879910675 18-19 22.850021662228333 27.39165709171225 27.413136553152178 22.345184692907235 20-21 23.483052148547447 26.55795843865941 27.567731003969115 22.39125840882403 22-23 23.368189319228254 26.331397766401825 27.292694724467598 23.007718189902317 24-25 23.200657862097888 26.537751783436086 27.23239580905358 23.02919454541245 26-27 22.958194128693414 26.90062969154672 27.118674164262956 23.02250201549691 28-29 22.93598708515184 26.748512921375205 27.31600040032548 22.999499593147473 30-31 23.05154563092804 26.605449815230266 27.04847596949067 23.294528584351028 32-33 22.92319970895596 26.788985236303564 26.929198140590632 23.35861691414984 34-35 23.322455078458862 26.469199765909796 27.200279070058908 23.00806608557243 36-37 23.274305458243415 26.387059904221065 27.008590720434377 23.33004391710114 38-39 23.038648732899905 26.571845852037544 27.28750124230619 23.10200417275636 40-41 23.291967151952253 26.45323223599006 27.063218163677206 23.191582448380483 42-43 23.099664808514728 26.541191510339136 26.77683168151378 23.58231199963236 44-45 23.17998998670697 26.669897659688697 26.694652358381187 23.455459995223144 46-47 23.243609053180624 26.95119108934036 26.31536439889235 23.489835458586672 48-49 23.398619370835476 26.948087389358772 26.1208101877765 23.532483052029256 50-51 23.276879427686204 26.92308227881151 26.24354667455745 23.55649161894484 52-53 23.612794594041386 26.793306742618917 26.05907998170351 23.534818681636185 54-55 23.396598561887494 26.66101472146036 26.246571622302667 23.695815094349477 56-57 23.321346264978033 27.01236536305848 26.15328246081865 23.51300591114484 58-59 23.46162281083452 26.571952225629815 26.182332116114974 23.784092847420695 60-61 23.556627772686486 26.615910414169974 26.32736912554253 23.50009268760101 62-63 23.495225447196482 26.850417773370307 26.269215555459606 23.385141223973605 64-65 23.40525871323964 26.546267425652374 26.179836719076 23.868637142031982 66-67 23.29938982446662 26.936214238171758 26.086622785642078 23.677773151719546 68-69 23.18435350570349 26.744103656771255 26.47314789007385 23.5983949474514 70-71 23.081024168577976 26.670219785709325 26.440392627776955 23.808363417935745 72-73 23.35646616529574 26.779651811625044 26.063176357685215 23.800705665394002 74-75 23.278685785389015 26.93664438609014 26.051304330118047 23.7333654984028 76-77 23.461526611763244 26.603129644802255 26.31891713781574 23.616426605618766 78-79 23.292919366486398 26.772429803816728 26.2849697256414 23.649681104055478 80-81 23.172734879133852 26.988487868999922 26.254130217851312 23.58464703401491 82-83 23.39317050687715 26.840587489717606 26.126903265266765 23.63933873813848 84-85 23.34454353109742 26.77778118148824 26.10036544246805 23.77730984494629 86-87 23.4047726967324 26.66861270263665 26.383687275152777 23.54292732547817 88-89 23.344681271827923 26.553810501496944 26.257241448223496 23.844266778451637 90-91 23.27147342277771 26.836638374221412 26.31141698876786 23.580471214233015 92-93 23.359411335488918 26.743842374821412 26.227069042824873 23.669677246864794 94-95 23.26336127712263 26.454862121843092 26.32340119416871 23.958375406865564 96-97 23.44598585624256 26.447047256054308 26.24061656936153 23.866350318341606 98-99 23.14298644109098 26.504516735418516 26.185351504708198 24.16714531878231 100-101 23.052280252595107 26.408941162217403 26.493364661124076 24.045413924063414 102-103 23.329609751444817 26.311944726217522 26.38555189888752 23.97289362345014 104-105 23.31418832243684 26.41050714030349 26.44199388923835 23.833310648021314 106-107 23.13258938253151 26.413287796312922 26.613397032249665 23.840725788905903 108-109 23.13882984611593 26.390007665310677 26.21092414684257 24.260238341730826 110-111 23.00465405939143 26.487827369364247 26.478706881982113 24.028811689262213 112-113 23.192003152454742 26.45298017671291 26.494613744229262 23.860402926603083 114-115 23.123391311673203 26.591631095958412 26.3232756009889 23.961701991379485 116-117 22.79730231191618 26.482151522514503 26.614327656176357 24.106218509392967 118-119 22.982790994617574 26.558526143861382 26.247144654368288 24.211538207152756 120-121 22.8439913794814 26.4904979007415 26.53923286295972 24.126277856817378 122-123 23.039497584487247 26.472996314941927 26.413034926942835 24.07447117362799 124-125 23.026873918989143 26.512031155744776 26.19418893828304 24.26690598698304 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 957.0 1 919.5 2 1077.0 3 1086.5 4 785.5 5 583.5 6 513.0 7 612.0 8 860.0 9 1022.5 10 986.0 11 973.0 12 1041.0 13 1159.5 14 1302.5 15 1467.0 16 1590.0 17 1722.5 18 1907.0 19 2118.0 20 2510.5 21 3041.0 22 3728.0 23 4791.5 24 6077.5 25 7644.5 26 10062.5 27 13192.5 28 16876.5 29 21210.5 30 26793.5 31 32792.0 32 38496.0 33 44433.0 34 50228.0 35 55121.0 36 61439.5 37 68773.0 38 72793.5 39 74477.5 40 76594.5 41 80757.5 42 85155.0 43 89809.0 44 94466.0 45 97721.5 46 98768.5 47 100660.0 48 106782.5 49 111037.0 50 111045.5 51 109560.5 52 109487.5 53 110934.5 54 109640.0 55 103280.5 56 95884.5 57 88670.0 58 82387.0 59 75940.0 60 65881.5 61 53389.0 62 41620.0 63 32206.5 64 24339.5 65 18403.0 66 14135.0 67 11080.5 68 8600.5 69 6571.5 70 5028.0 71 3892.0 72 2976.0 73 2393.0 74 1942.5 75 1444.0 76 1016.5 77 679.0 78 491.0 79 348.5 80 228.0 81 165.0 82 119.5 83 87.5 84 74.0 85 59.0 86 38.5 87 35.5 88 27.5 89 18.5 90 21.5 91 21.5 92 14.5 93 12.0 94 11.0 95 7.5 96 5.5 97 5.5 98 3.0 99 5.0 100 10.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012740707116207084 2 0.005221601277134051 3 0.008528615419318951 4 0.0088071008207661 5 0.014829347627060705 6 0.0011487522809694912 7 0.00786721259088197 8 0.0 9 0.0029589073903759622 10-11 0.0 12-13 0.003881390282669645 14-15 0.01965062613961448 16-17 0.031869173128108164 18-19 0.005935220118342371 20-21 0.0088071008207661 22-23 0.001827560446996918 24-25 0.012096709625360552 26-27 0.019546194114071797 28-29 6.788081660274267E-4 30-31 0.001827560446996918 32-33 0.009207423585346377 34-35 0.009921042426554696 36-37 0.011783413548732509 38-39 1.7405337590446836E-5 40-41 0.02542919821964283 42-43 0.010234338503182741 44-45 0.017405337590446837 46-47 0.0042643077096594754 48-49 0.02542919821964283 50-51 0.004595009123877965 52-53 0.0 54-55 0.017910092380569793 56-57 0.004943115875686901 58-59 4.1772810217072405E-4 60-61 0.004507982435925731 62-63 0.027500433392906004 64-65 0.01794490305575069 66-67 0.0022975045619389825 68-69 0.021808888000829887 70-71 0.00308074475350909 72-73 0.01244481637716949 74-75 1.7405337590446836E-5 76-77 0.00638775889569399 78-79 0.001270589644102619 80-81 0.003602904881222495 82-83 0.0018449657845873648 84-85 0.0 86-87 0.0061266788318372865 88-89 0.01744014826562773 90-91 0.022800992243485356 92-93 0.005082358576410476 94-95 0.025272550181328804 96-97 0.018501873858644987 98-99 0.0011139416057885975 100-101 0.009015964871851462 102-103 0.0 104-105 0.0015664803831402154 106-107 3.481067518089367E-5 108-109 0.0 110-111 9.921042426554697E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 3.481067518089367E-5 122-123 0.0 124-125 0.022957640281799377 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2872682.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.53819287221675 #Duplication Level Percentage of deduplicated Percentage of total 1 76.61334293814288 38.7189990199315 2 10.97340659714167 11.091522781432028 3 4.388672726561276 6.653867661239733 4 2.401200779837717 4.854093925454232 5 1.3910911044641445 3.5151615270116974 6 0.8871684621141296 2.690153450908308 7 0.642845847104078 2.2741787185634617 8 0.4412537645235199 1.784013428566509 9 0.33261661183484986 1.5128858233271605 >10 1.7423329196234034 15.907524780486959 >50 0.12069698331808663 4.222351711928482 >100 0.062409053290501525 5.528597908117985 >500 0.0024685100364902042 0.8217013712919792 >1k 4.937020072814043E-4 0.4249478917399395 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3205 0.11156821395476424 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.481067518089367E-5 2 0.0 0.0 0.0 0.0 3.481067518089367E-5 3 0.0 0.0 0.0 0.0 3.481067518089367E-5 4 0.0 0.0 0.0 0.0 6.962135036178734E-5 5 0.0 0.0 0.0 0.0 6.962135036178734E-5 6 0.0 0.0 0.0 0.0 6.962135036178734E-5 7 0.0 0.0 0.0 3.481067518089367E-5 6.962135036178734E-5 8 0.0 0.0 0.0 3.481067518089367E-5 6.962135036178734E-5 9 0.0 0.0 0.0 3.481067518089367E-5 6.962135036178734E-5 10-11 3.481067518089367E-5 0.0 0.0 3.481067518089367E-5 1.0443202554268103E-4 12-13 3.481067518089367E-5 0.0 0.0 3.481067518089367E-5 1.2183736313312786E-4 14-15 1.3924270072357468E-4 0.0 0.0 3.481067518089367E-5 2.0886405108536203E-4 16-17 1.566480383140215E-4 0.0 0.0 3.481067518089367E-5 3.1329607662804307E-4 18-19 1.7405337590446836E-4 0.0 0.0 3.481067518089367E-5 3.1329607662804307E-4 20-21 1.7405337590446836E-4 0.0 0.0 3.481067518089367E-5 3.1329607662804307E-4 22-23 1.7405337590446836E-4 0.0 0.0 1.2183736313312785E-4 3.4810675180893676E-4 24-25 2.4367472626625572E-4 0.0 0.0 3.307014142184899E-4 4.1772810217072405E-4 26-27 2.4367472626625572E-4 0.0 0.0 5.569708028942987E-4 4.8734945253251145E-4 28-29 2.4367472626625572E-4 0.0 0.0 0.001079130930607704 4.8734945253251145E-4 30-31 2.4367472626625572E-4 0.0 0.0 0.003011123403147303 4.8734945253251145E-4 32-33 2.4367472626625572E-4 0.0 0.0 0.006596622946779351 4.8734945253251145E-4 34-35 2.6108006385670255E-4 0.0 0.0 0.011957466924636978 5.221601277134051E-4 36-37 2.7848540144714937E-4 0.0 0.0 0.01982467951551895 5.221601277134051E-4 38-39 3.481067518089367E-4 0.0 0.0 0.03451478444185607 5.221601277134051E-4 40-41 3.481067518089367E-4 0.0 0.0 0.05230303945929274 5.221601277134051E-4 42-43 3.6551208939938353E-4 0.0 0.0 0.07035237454058611 5.221601277134051E-4 44-45 3.829174269898304E-4 0.0 0.0 0.08982894730429612 5.221601277134051E-4 46-47 4.8734945253251145E-4 0.0 0.0 0.11407458256778857 5.39565465303852E-4 48-49 4.8734945253251145E-4 0.0 0.0 0.1444468966631183 5.569708028942987E-4 50-51 4.8734945253251145E-4 0.0 0.0 0.17755184876014818 5.569708028942987E-4 52-53 4.8734945253251145E-4 0.0 0.0 0.20980393931524616 6.43997490846533E-4 54-55 4.8734945253251145E-4 0.0 0.0 0.24649439095590808 7.48429516389214E-4 56-57 5.221601277134051E-4 0.0 0.0 0.29046027370937677 7.658348539796608E-4 58-59 5.221601277134051E-4 0.0 0.0 0.34142310217420513 8.180508667510013E-4 60-61 5.221601277134051E-4 0.0 0.0 0.3964413742976076 8.702668795223419E-4 62-63 5.221601277134051E-4 0.0 0.0 0.45299131612896937 8.702668795223419E-4 64-65 5.221601277134051E-4 0.0 0.0 0.5137185389820383 8.702668795223419E-4 66-67 5.569708028942987E-4 0.0 0.0 0.5886311119713216 8.702668795223419E-4 68-69 5.569708028942987E-4 0.0 0.0 0.6696007424420802 8.702668795223419E-4 70-71 5.569708028942987E-4 3.481067518089367E-5 0.0 0.7616575729579536 8.702668795223419E-4 72-73 5.569708028942987E-4 3.481067518089367E-5 0.0 0.857108444303964 9.050775547032355E-4 74-75 5.569708028942987E-4 3.481067518089367E-5 0.0 0.9574154048377091 9.050775547032355E-4 76-77 5.743761404847456E-4 3.481067518089367E-5 0.0 1.0681655679257225 9.050775547032355E-4 78-79 5.917814780751925E-4 3.481067518089367E-5 0.0 1.1988274372172067 9.224828922936824E-4 80-81 6.091868156656393E-4 3.481067518089367E-5 0.0 1.3440227633967141 9.921042426554697E-4 82-83 6.265921532560861E-4 3.481067518089367E-5 0.0 1.5016629059533915 0.0011487522809694912 84-85 6.614028284369798E-4 5.2216012771340507E-5 0.0 1.6649075672141922 0.0011661576185599382 86-87 6.614028284369798E-4 6.962135036178734E-5 0.0 1.8353580382374381 0.001183562956150385 88-89 6.614028284369798E-4 6.962135036178734E-5 0.0 2.0263119969422303 0.0012009682937408317 90-91 7.310241787987672E-4 1.0443202554268103E-4 0.0 2.240728350718945 0.0012183736313312785 92-93 7.310241787987672E-4 1.0443202554268103E-4 0.0 2.4828365966020605 0.0012183736313312785 94-95 7.658348539796608E-4 1.0443202554268103E-4 0.0 2.7332994045285903 0.0012531843065121723 96-97 7.658348539796608E-4 1.0443202554268103E-4 0.0 2.986581877144773 0.0012531843065121723 98-99 8.354562043414481E-4 1.0443202554268103E-4 0.0 3.2643362544131236 0.0012531843065121723 100-101 9.57293567474576E-4 1.0443202554268103E-4 0.0 3.5804868064059994 0.0012531843065121723 102-103 0.001079130930607704 1.0443202554268103E-4 0.0 3.922658338096594 0.0013054003192835128 104-105 0.0013228056568739596 1.0443202554268103E-4 0.0 4.267753966502383 0.0013228056568739596 106-107 0.0013228056568739596 1.0443202554268103E-4 0.0 4.6241282536667825 0.001357616332054853 108-109 0.0013228056568739596 1.0443202554268103E-4 0.0 4.9979600944344 0.0014446430200070874 110-111 0.0013750216696453 1.0443202554268103E-4 0.0 5.41046659532799 0.0014794536951879811 112-113 0.0013924270072357468 1.0443202554268103E-4 0.0 5.855782157579572 0.0015142643703688749 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7235 0.0 48.189743 1 GGTATCA 4260 0.0 46.647934 1 ATCAACG 8270 0.0 40.574577 3 TCAACGC 8445 0.0 39.66333 4 CAACGCA 8485 0.0 39.40623 5 TATCAAC 8840 0.0 38.698666 2 AACGCAG 8650 0.0 38.510956 6 GTACATG 10665 0.0 34.197563 1 ACGCAGA 10045 0.0 32.68956 7 TACATGG 11020 0.0 31.637085 2 CGCAGAG 10330 0.0 30.696737 8 ACATGGG 11500 0.0 28.91974 3 GCAGAGT 11200 0.0 27.198656 9 CATGGGG 6160 0.0 24.532026 4 GAGTACT 4960 0.0 23.457085 12-13 AGAGTAC 9885 0.0 22.935894 10-11 CAGAGTA 10995 0.0 22.162872 10-11 GAGTACA 8625 0.0 21.729362 1 GTACTTT 5455 0.0 21.273983 14-15 AGTACAT 8040 0.0 21.23764 2 >>END_MODULE