FastQCFastQC Report
Thu 26 May 2016
SRR1364585_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364585_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2872682
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55210.19218973767371397No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47040.16374941605092386No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30240.10526748174702247No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57850.046.2761341
GTATCAA91550.043.5694271
ATCAACG114400.034.623473
TCAACGC116500.033.9993554
CAACGCA117000.033.6649635
AACGCAG118850.033.1916
TATCAAC118700.033.0681272
GTACATG108350.031.5311491
TACATGG110550.029.9026152
ACGCAGA138400.028.2015977
CGCAGAG141250.027.5483288
ACATGGG116850.027.52583
GAGTACT79200.024.67792312-13
GCAGAGT151800.024.6138049
CATGGGG64750.023.3651684
AGAGTAC133700.023.25061210-11
GTACTTT86950.022.64940614-15
AGTACTT86700.022.4745312-13
CAGAGTA149650.021.01105110-11
CATGGGA66300.020.2136124
Produced by FastQC (version 0.11.5)