##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364585_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2872682 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.890920053107166 31.0 30.0 34.0 26.0 34.0 2 29.83310683187349 31.0 30.0 34.0 26.0 34.0 3 30.09494646466264 31.0 30.0 34.0 26.0 34.0 4 33.64037265523994 35.0 35.0 37.0 28.0 37.0 5 33.07162122365093 35.0 33.0 37.0 26.0 37.0 6 33.07609265487792 35.0 33.0 37.0 26.0 37.0 7 32.86068628549906 35.0 33.0 37.0 25.0 37.0 8 32.78952595518752 35.0 33.0 37.0 25.0 37.0 9 34.03055855120755 38.0 34.0 39.0 25.0 39.0 10-11 33.959114339839914 38.0 34.0 39.0 25.0 39.0 12-13 33.846321486332286 38.0 33.0 39.0 24.0 39.0 14-15 34.79445949812754 39.0 34.0 41.0 23.5 41.0 16-17 34.78472643334696 39.0 34.0 41.0 24.0 41.0 18-19 34.76782776513377 39.0 33.5 41.0 23.5 41.0 20-21 34.766183830998344 39.0 34.0 41.0 23.0 41.0 22-23 34.7165495519518 39.0 34.0 41.0 23.0 41.0 24-25 34.66557575812429 39.0 34.0 41.0 23.0 41.0 26-27 34.54715819572093 39.0 34.0 41.0 23.0 41.0 28-29 34.391375550791906 39.0 33.0 41.0 21.5 41.0 30-31 34.24962682956206 38.0 33.0 40.5 21.0 41.0 32-33 34.073707079307766 38.0 33.0 40.0 19.5 41.0 34-35 33.90353300504546 38.0 33.0 40.0 18.0 41.0 36-37 33.71480971440626 38.0 33.0 40.0 17.0 41.0 38-39 33.54904998186363 38.0 32.5 40.0 16.0 41.0 40-41 33.59286704898071 38.0 33.0 40.0 15.5 41.0 42-43 33.47780993510594 38.0 33.0 40.0 15.0 41.0 44-45 33.33375344016497 38.0 32.0 40.0 13.5 41.0 46-47 33.1270225872547 38.0 32.0 40.0 9.0 41.0 48-49 32.87997540277692 38.0 32.0 40.0 7.5 41.0 50-51 32.71370099440175 38.0 31.5 40.0 6.5 41.0 52-53 32.52177912487355 38.0 31.0 40.0 2.0 41.0 54-55 32.32350343685796 37.0 31.0 40.0 2.0 41.0 56-57 32.09315632569147 37.0 31.0 40.0 2.0 41.0 58-59 31.86731911154802 37.0 31.0 40.0 2.0 41.0 60-61 31.612268604739405 36.0 30.0 40.0 2.0 41.0 62-63 31.426113297608296 36.0 30.0 39.5 2.0 41.0 64-65 31.272943890065104 35.5 30.0 39.0 2.0 41.0 66-67 30.95967513981708 35.0 30.0 39.0 2.0 41.0 68-69 30.66858688152744 35.0 30.0 38.5 2.0 40.5 70-71 30.349967730504105 35.0 29.0 37.5 2.0 40.0 72-73 30.059737207250926 35.0 29.0 37.0 2.0 39.5 74-75 29.71208090557883 35.0 29.0 36.5 2.0 39.0 76-77 29.4060811464687 34.5 29.0 36.0 2.0 39.0 78-79 29.13383103315995 34.0 29.0 36.0 2.0 38.0 80-81 28.8688203219152 34.0 29.0 35.0 2.0 37.0 82-83 28.599631285328485 34.0 29.0 35.0 2.0 37.0 84-85 28.392310217420516 34.0 28.5 35.0 2.0 36.0 86-87 28.194289169493874 34.0 28.0 35.0 2.0 36.0 88-89 28.033896198743893 34.0 28.0 35.0 2.0 36.0 90-91 27.901420693275483 34.0 27.5 35.0 2.0 35.0 92-93 27.795267453898482 34.0 27.0 35.0 2.0 35.0 94-95 27.69328383719465 34.0 27.0 35.0 2.0 35.0 96-97 27.58319229208106 34.0 27.0 35.0 2.0 35.0 98-99 27.506368613024346 34.0 27.0 35.0 2.0 35.0 100-101 27.404729447951425 34.0 27.0 35.0 2.0 35.0 102-103 26.131056274241285 31.5 24.5 34.0 2.0 35.0 104-105 27.07398452038896 33.5 25.5 35.0 2.0 35.0 106-107 27.23707462225196 34.0 27.0 35.0 2.0 35.0 108-109 27.247195652007427 34.0 27.0 35.0 2.0 35.0 110-111 27.17223434407289 34.0 27.0 35.0 2.0 35.0 112-113 27.089497027516444 34.0 26.0 35.0 2.0 35.0 114-115 26.983730360687332 34.0 25.5 35.0 2.0 35.0 116-117 26.855691649824102 34.0 25.0 35.0 2.0 35.0 118-119 26.71872504509723 34.0 25.0 35.0 2.0 35.0 120-121 26.514676354709643 34.0 25.0 35.0 2.0 35.0 122-123 26.215175922709165 34.0 25.0 35.0 2.0 35.0 124-125 24.387517309608235 31.5 13.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7947885689063234 1101 2 0.9394942636772186 1101 3 0.780068292767826 1101 4 0.7311879400322638 1101 5 0.8608519549627331 1101 6 0.8973531891158331 1101 7 0.9274291848727003 1101 8 0.9471758600428544 1101 9 1.1692440678912064 1101 10-11 1.201064407762864 1101 12-13 1.1567403570001105 1101 14-15 1.38400887618171 1101 16-17 1.3562866350570673 1101 18-19 1.386147639569387 1101 20-21 1.3724418589421816 1101 22-23 1.4435927443959642 1101 24-25 1.4133370988559406 1101 26-27 1.323846798482741 1101 28-29 1.4544041013807316 1101 30-31 1.4619277308000562 1101 32-33 1.531676792082088 1101 34-35 1.5704641002952044 1101 36-37 1.6642507413047554 1101 38-39 1.5411248089827865 1101 40-41 1.343920797465067 1101 42-43 1.3407227083521889 1101 44-45 1.3304312331642478 1101 46-47 1.4196943998621236 1101 48-49 1.3460063019206174 1101 50-51 1.3936791795776458 1101 52-53 1.4409707701486028 1101 54-55 1.4646642963546483 1101 56-57 1.40798339254345 1101 58-59 1.4064249602895025 1101 60-61 1.31792484120286 1101 62-63 1.3584487304283854 1101 64-65 1.2625944623249268 1101 66-67 1.3728269841360294 1101 68-69 1.4361112036120716 1101 70-71 1.4543566587664678 1101 72-73 1.4236972910303116 1101 74-75 1.4671832939530525 1101 76-77 1.3816320974599918 1101 78-79 1.3879999189140797 1101 80-81 1.253403846812386 1101 82-83 1.406840042821326 1101 84-85 1.4007168017099438 1101 86-87 1.4650969482555531 1101 88-89 1.4386363567012346 1101 90-91 1.3467191724668446 1101 92-93 1.353801574205221 1101 94-95 1.295345513173821 1101 96-97 1.3172399480931745 1101 98-99 1.3129300324243118 1101 100-101 1.244961562665413 1101 102-103 1.2172592242661722 1101 104-105 1.3103555114853584 1101 106-107 1.1914125959068755 1101 108-109 1.2482846523645108 1101 110-111 1.2310111495874487 1101 112-113 1.328603039050055 1101 114-115 1.2591283062499983 1101 116-117 1.2656408815683378 1101 118-119 1.2288947393521426 1101 120-121 1.2816258064809496 1101 122-123 1.1789690006948739 1101 124-125 1.6152801395172212 1102 1 0.4239199640789586 1102 2 -0.3323421056233009 1102 3 0.3620301120902596 1102 4 0.3826532050189755 1102 5 0.5829644860020196 1102 6 0.46196402857503926 1102 7 0.39063670475157153 1102 8 0.5986505241471107 1102 9 0.8981414563672772 1102 10-11 0.9202697134185129 1102 12-13 0.963633972670408 1102 14-15 1.16408231879754 1102 16-17 1.111096032963168 1102 18-19 1.3056985298903498 1102 20-21 1.077415073023694 1102 22-23 1.3008373544458962 1102 24-25 1.2191619565350251 1102 26-27 1.305025587765698 1102 28-29 0.9082330773353817 1102 30-31 1.1605561750153015 1102 32-33 1.2534123662903696 1102 34-35 1.3518135775528322 1102 36-37 0.9464935524680271 1102 38-39 0.3633657998502926 1102 40-41 -0.02479235473055752 1102 42-43 0.29511341212364073 1102 44-45 -0.026624010775748275 1102 46-47 0.27495731041165783 1102 48-49 -0.10300137057903669 1102 50-51 -0.19603719891730265 1102 52-53 -0.5082748044596528 1102 54-55 -0.30284437981411116 1102 56-57 -0.2771322275680319 1102 58-59 -0.14671370792029137 1102 60-61 -0.27703671483985204 1102 62-63 -0.124251567116346 1102 64-65 -0.0821232388347255 1102 66-67 -0.32452928951907367 1102 68-69 -0.11869465918149302 1102 70-71 -0.708019049652858 1102 72-73 -0.4205340841222025 1102 74-75 -0.26600860354810507 1102 76-77 -0.6747842780167872 1102 78-79 -0.5657273638104385 1102 80-81 -0.528329271093174 1102 82-83 -0.7473002893455778 1102 84-85 -0.6525430106815797 1102 86-87 -0.3785611068351784 1102 88-89 -0.47440118013678045 1102 90-91 -0.4773124286195696 1102 92-93 -0.32393571252227105 1102 94-95 -0.09604324508474349 1102 96-97 -0.3302416314640375 1102 98-99 -0.5514837284268381 1102 100-101 -0.3393206899103909 1102 102-103 -0.3988968196744942 1102 104-105 -0.3472041413195903 1102 106-107 -0.22090817989210976 1102 108-109 -0.24066783373863032 1102 110-111 -0.11667014555246524 1102 112-113 -0.041585121884143206 1102 114-115 -0.02489785692473845 1102 116-117 -0.06031163805832662 1102 118-119 0.1025569707841072 1102 120-121 0.23680258978335544 1102 122-123 0.1692169723914141 1102 124-125 0.4218409219916701 1103 1 0.4007883979243587 1103 2 0.06384149608645018 1103 3 0.44030635718807787 1103 4 0.45119718424404454 1103 5 0.5744154188336097 1103 6 0.6185958604613333 1103 7 0.6833387489965261 1103 8 0.7772472530025496 1103 9 0.8840589359714315 1103 10-11 0.9078810751486159 1103 12-13 0.8431345671033839 1103 14-15 0.9984729116436881 1103 16-17 0.9431078706174745 1103 18-19 1.003946023070739 1103 20-21 0.993028337065283 1103 22-23 0.9859115294812213 1103 24-25 0.8820055029070986 1103 26-27 0.8908455117236187 1103 28-29 1.0246322963864571 1103 30-31 0.9698944716121787 1103 32-33 0.9485885961495413 1103 34-35 0.9504835494935833 1103 36-37 1.0616420379233062 1103 38-39 1.0830590843947832 1103 40-41 0.9802734705363676 1103 42-43 0.8929236916582752 1103 44-45 0.874376843721663 1103 46-47 0.8763988103978022 1103 48-49 0.8449400116539678 1103 50-51 0.7746119640908304 1103 52-53 0.8976376785070741 1103 54-55 0.9592627843627355 1103 56-57 1.0531842324324217 1103 58-59 1.111790178563158 1103 60-61 1.1072485456149934 1103 62-63 0.9333415271340257 1103 64-65 0.8913028833464409 1103 66-67 0.8897402663185865 1103 68-69 0.9395238018667769 1103 70-71 0.9241209153864212 1103 72-73 0.9269108930450223 1103 74-75 1.008000443923411 1103 76-77 1.0178224774873819 1103 78-79 0.8723667543255615 1103 80-81 0.9255745447383887 1103 82-83 0.995953305588646 1103 84-85 1.045005205137894 1103 86-87 1.0326365717277 1103 88-89 1.0453165680956644 1103 90-91 1.05700112092617 1103 92-93 1.0690956312237105 1103 94-95 1.0277526975323248 1103 96-97 1.0704797167401985 1103 98-99 1.02914411622465 1103 100-101 1.0093251961599776 1103 102-103 0.8092107676580369 1103 104-105 0.7784683739631255 1103 106-107 0.9050238488371392 1103 108-109 0.9128934432343918 1103 110-111 0.8381446829057602 1103 112-113 0.8011929424052973 1103 114-115 0.9201660509179987 1103 116-117 0.9540448787910769 1103 118-119 0.8638753620620605 1103 120-121 0.9392661335358916 1103 122-123 0.9628086983124753 1103 124-125 1.275356010766206 1104 1 0.41512265743957855 1104 2 0.5476064372668112 1104 3 0.2645704324356295 1104 4 -0.0187559514250637 1104 5 0.33940237668051765 1104 6 0.41176786803145404 1104 7 0.580395374859215 1104 8 0.4315597578756325 1104 9 0.5279396380740025 1104 10-11 0.5235167737898294 1104 12-13 0.5468330039351841 1104 14-15 0.6715891829110205 1104 16-17 0.6265414271086627 1104 18-19 0.5688759214978916 1104 20-21 0.5086219942551224 1104 22-23 0.5789354395045407 1104 24-25 0.6372856404403819 1104 26-27 0.55987038336945 1104 28-29 0.6859724573578063 1104 30-31 0.6258564426311892 1104 32-33 0.7133588492901666 1104 34-35 0.8275010813842485 1104 36-37 0.7639035785748973 1104 38-39 0.9556525915525995 1104 40-41 0.7790789882885818 1104 42-43 0.9387767093100763 1104 44-45 0.8115666643448094 1104 46-47 0.8349592591234796 1104 48-49 0.8833646725461719 1104 50-51 0.7058437712323595 1104 52-53 0.784037527411833 1104 54-55 0.4504097141406618 1104 56-57 0.6341926602150423 1104 58-59 0.7747029438067621 1104 60-61 0.8440313305635954 1104 62-63 0.8361070483594233 1104 64-65 0.8084931115368619 1104 66-67 0.8252194857031938 1104 68-69 0.6046236233641196 1104 70-71 0.23324844378027976 1104 72-73 0.1984624898818268 1104 74-75 0.6647372357900245 1104 76-77 0.8061391921170404 1104 78-79 0.8612787091600183 1104 80-81 0.7505318535420784 1104 82-83 0.7506345702522097 1104 84-85 0.7119648477072964 1104 86-87 0.7477141837614489 1104 88-89 0.7545726130863635 1104 90-91 0.8146741657254921 1104 92-93 0.7337058551376359 1104 94-95 0.7057171736134435 1104 96-97 0.7335265657134791 1104 98-99 0.7870648024498195 1104 100-101 0.7475558786520686 1104 102-103 0.617599211176298 1104 104-105 0.6700404141386294 1104 106-107 0.6547728848519085 1104 108-109 0.6679837518750595 1104 110-111 0.6562287238704059 1104 112-113 0.6245800834802786 1104 114-115 0.5887540429225915 1104 116-117 0.6301781078108135 1104 118-119 0.5964615330087781 1104 120-121 0.5861051686764576 1104 122-123 0.7414235395502509 1104 124-125 1.095897638152838 1105 1 0.20123614739047468 1105 2 -0.017202034790333443 1105 3 0.2563331994205562 1105 4 0.2828051135837484 1105 5 -0.03175301761113758 1105 6 0.41529634317159747 1105 7 0.44745364656319 1105 8 0.5838928654127713 1105 9 0.7144099319851378 1105 10-11 0.6213433545844893 1105 12-13 0.7483227551011282 1105 14-15 0.7226996458626758 1105 16-17 0.7622566291004063 1105 18-19 0.7553738714082954 1105 20-21 0.7093994872894029 1105 22-23 0.8156727739738514 1105 24-25 0.7810914815341476 1105 26-27 0.8112203311360489 1105 28-29 0.8100584461438984 1105 30-31 0.9642399668349384 1105 32-33 0.9002889761512449 1105 34-35 0.9254673604316608 1105 36-37 1.062605927789427 1105 38-39 1.0428612248410545 1105 40-41 0.8157482024371276 1105 42-43 0.8199714758658132 1105 44-45 0.9750631612271121 1105 46-47 0.8983521156986853 1105 48-49 0.6730333888867293 1105 50-51 0.6237503240805253 1105 52-53 0.7686225967031959 1105 54-55 0.7386388269044204 1105 56-57 0.6335440035459072 1105 58-59 0.4636275548242743 1105 60-61 0.5954597225792355 1105 62-63 0.7710880874943484 1105 64-65 0.488092906975389 1105 66-67 0.6738760276821374 1105 68-69 0.7665784266634574 1105 70-71 0.8572642777812938 1105 72-73 0.7986247677541449 1105 74-75 0.7792601174737435 1105 76-77 0.7454083049433926 1105 78-79 0.7260308470959167 1105 80-81 0.6503598190440201 1105 82-83 0.5849847200469789 1105 84-85 0.5502602932997256 1105 86-87 0.668909159632264 1105 88-89 0.6815896047269199 1105 90-91 0.5495205976589901 1105 92-93 0.5875792820162253 1105 94-95 0.5538611870823793 1105 96-97 0.4759642637894892 1105 98-99 0.45052530496348453 1105 100-101 0.5432808186854992 1105 102-103 0.2488558308267912 1105 104-105 0.4367178253199562 1105 106-107 0.5098986810133539 1105 108-109 0.5768088668284044 1105 110-111 0.497251476468346 1105 112-113 0.5292688292687338 1105 114-115 0.48398710230258857 1105 116-117 0.2681321497510005 1105 118-119 0.47278549367260325 1105 120-121 0.5243068968658662 1105 122-123 0.4725628449459869 1105 124-125 0.8422589669377736 1106 1 -0.08314238835986032 1106 2 -0.37756196899417205 1106 3 0.04368012297284807 1106 4 -0.02874162993270346 1106 5 0.07652860039875264 1106 6 0.3877280276993318 1106 7 0.3681151741427797 1106 8 0.4140858308350204 1106 9 0.47064932789275105 1106 10-11 0.4081264532506381 1106 12-13 0.4284791463226796 1106 14-15 0.46627222409669145 1106 16-17 0.4622000570950675 1106 18-19 0.5431625710769552 1106 20-21 0.5233938771900952 1106 22-23 0.4889100865114884 1106 24-25 0.451815878128059 1106 26-27 0.617440896662039 1106 28-29 0.6378312577412402 1106 30-31 0.5518171860555441 1106 32-33 0.5638451932802013 1106 34-35 0.7280827872344418 1106 36-37 0.5743354715564664 1106 38-39 0.6325598437240316 1106 40-41 0.6284516296878664 1106 42-43 0.5353604915371761 1106 44-45 0.5680994981023417 1106 46-47 0.5319966051689278 1106 48-49 0.6433567125026585 1106 50-51 0.7152637298365434 1106 52-53 0.5629880320468672 1106 54-55 0.525588119102423 1106 56-57 0.17243799001349913 1106 58-59 0.5666876080749397 1106 60-61 0.5926891396985781 1106 62-63 0.5127543043746599 1106 64-65 0.618959131820457 1106 66-67 0.5205240479247486 1106 68-69 0.5447229487811178 1106 70-71 0.6438875254085907 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-0.13024033390913559 2207 88-89 -0.3397981135090653 2207 90-91 -0.7693873639693258 2207 92-93 -0.5087728834703356 2207 94-95 -0.4571517863095522 2207 96-97 -0.30046317960156443 2207 98-99 -0.36711563213594545 2207 100-101 -1.0984276188659905 2207 102-103 -0.8262219577460357 2207 104-105 -0.6332485656414768 2207 106-107 -0.9916963509941539 2207 108-109 -1.120724261048906 2207 110-111 -0.6091888192968149 2207 112-113 -0.5518577935090718 2207 114-115 -0.858948742996894 2207 116-117 -0.963560567524663 2207 118-119 -0.7170365358665833 2207 120-121 -0.3654116453730687 2207 122-123 -0.34794897917798195 2207 124-125 -0.7332736921208927 2208 1 0.02667385801036204 2208 2 0.26863769896984735 2208 3 0.03604908393415229 2208 4 0.10338858020954689 2208 5 -0.024822638052491186 2208 6 -0.06971747937165418 2208 7 -0.3067366210834024 2208 8 -0.16403243699222259 2208 9 0.029309714281424704 2208 10-11 -0.0015335321168592486 2208 12-13 -0.006144406987480977 2208 14-15 0.05748081955584183 2208 16-17 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-1.7269263905045555 2216 102-103 -1.5422242778396154 2216 104-105 -1.6204158047020663 2216 106-107 -1.6315197776687995 2216 108-109 -1.6946997817677776 2216 110-111 -1.7746457891836762 2216 112-113 -1.7005533093237126 2216 114-115 -1.7639028119153508 2216 116-117 -1.8352764060448656 2216 118-119 -1.7266383997144956 2216 120-121 -1.9241223737173776 2216 122-123 -2.0043630482687327 2216 124-125 -2.350481815794037 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 100708.0 3 32341.0 4 15999.0 5 12371.0 6 12981.0 7 15191.0 8 16867.0 9 18188.0 10 19474.0 11 20710.0 12 22292.0 13 24417.0 14 26919.0 15 23896.0 16 17289.0 17 15889.0 18 14929.0 19 14363.0 20 14146.0 21 14735.0 22 17097.0 23 21118.0 24 27284.0 25 35914.0 26 44163.0 27 50876.0 28 59131.0 29 69568.0 30 80268.0 31 95185.0 32 116056.0 33 143013.0 34 184740.0 35 255506.0 36 418469.0 37 608159.0 38 192047.0 39 383.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.49943533181617 19.707633944485156 14.392435502209716 24.40049522148896 2 15.594312214160844 20.86823393609178 38.33804089697363 25.19941295277375 3 19.088538167468588 24.899797471491798 29.140921271480796 26.870743089558818 4 13.315480909114669 17.326457188156073 35.903234517784185 33.45482738494508 5 13.871810384859861 38.061748568062875 33.82675144690571 14.239689600171548 6 32.52914871886272 36.79933943262777 16.732516860550522 13.938994987958989 7 28.560612099896854 32.19264896680815 20.835249423606395 18.4114895096886 8 26.197015212189807 35.66528656754972 19.302252150479426 18.83544606978105 9 26.329067566584357 15.98693012049535 19.459687338014643 38.224314974905646 10-11 25.248145576649645 26.694697611985553 26.938687630290897 21.118469181073905 12-13 25.92161262541416 23.744239703524443 27.555921609144345 22.77822606191705 14-15 23.020742644240695 25.0897680598716 25.144055326713964 26.745433969173742 16-17 22.94614703930902 28.381849106442292 25.68645102089551 22.985552833353175 18-19 22.846240901589038 26.86170833076599 27.911813513601015 22.380237254043962 20-21 23.4616209374393 26.136500507896237 27.944753839159457 22.457124715505003 22-23 23.336606697156174 25.86603738248786 27.811014236869937 22.986341683486025 24-25 23.225995776768887 26.135924547165335 27.642269488930555 22.995810187135227 26-27 22.98421823229999 26.50617088838932 27.59651085640527 22.913100022905425 28-29 22.893484207440988 26.3145381215185 27.889999658855384 22.90197801218513 30-31 23.00559198686106 26.10231484027818 27.7387472751944 23.153345897666362 32-33 22.88920249439374 26.22782472964289 27.640772629897775 23.242200146065592 34-35 23.29587820719453 26.11679608115343 27.58375274395147 23.003572967700567 36-37 23.32080265062405 26.014992261586904 27.439514711339434 23.2246903764496 38-39 23.083654925954214 26.226850030737825 27.655271276110614 23.034223767197343 40-41 23.191533208339802 25.94502280447331 27.738416573780185 23.125027413406706 42-43 23.089833820798823 26.113158365597027 27.339242561480877 23.457765252123277 44-45 23.193151904735714 26.200393917600344 27.235820045518437 23.3706341321455 46-47 23.185128044106516 26.490488679220327 26.958152694938043 23.366230581735117 48-49 23.409273981596293 26.5486573174476 26.620663199059276 23.42140550189683 50-51 23.28919861112344 26.53284048370834 26.66329351165453 23.51466739351369 52-53 23.610444873466676 26.501401825889538 26.419231227125035 23.46892207351875 54-55 23.39474052470827 26.352151056051454 26.636101733502006 23.617006685738275 56-57 23.311426397080684 26.66733740557725 26.565542912088407 23.455693285253652 58-59 23.417931596740367 26.29154453083567 26.58120683110105 23.709317041322915 60-61 23.527073306408436 26.35423969656231 26.704330656856555 23.414356340172702 62-63 23.47000120444936 26.5222360149853 26.711693114657315 23.296069665908025 64-65 23.319405543406596 26.23623495043294 26.661744423488358 23.782615082672105 66-67 23.203943214041793 26.657214407999213 26.55650712470089 23.582335253258105 68-69 23.130461904669904 26.437650675858077 26.80986388501475 23.622023534457266 70-71 23.065396726821835 26.432441878356183 26.75487575721921 23.74728563760277 72-73 23.273947481830568 26.53823152023092 26.394759322472865 23.793061675465648 74-75 23.252522281275372 26.690326606379656 26.319936574188098 23.737214538156877 76-77 23.39326107101308 26.407987379041607 26.555219129719198 23.643532420226116 78-79 23.249649630554302 26.52390692739398 26.535133370139818 23.691310071911893 80-81 23.14975379249714 26.68864615375511 26.51758628750137 23.64401376624638 82-83 23.341897919783673 26.508015854173905 26.400938217317478 23.749148008724948 84-85 23.238736483885095 26.516631496281175 26.360244538031008 23.88438748180272 86-87 23.334048112530382 26.40586392785557 26.579238495594016 23.680849464020035 88-89 23.270452074253043 26.35895631069228 26.456010010316305 23.914581604738363 90-91 23.098884596345854 26.586270251980554 26.607574385191263 23.70727076648233 92-93 23.262582368444594 26.471666369028036 26.45641891644918 23.809332346078186 94-95 23.097474764001028 26.234873891367023 26.5448281431777 24.122823201454253 96-97 23.269635044856752 26.222753171844733 26.497730225177605 24.00988155812091 98-99 22.971993999783468 26.2467698706695 26.416583378676116 24.364652750870913 100-101 22.883333988948763 26.2132221502399 26.71210487711736 24.191338983693974 102-103 23.208921140592658 26.13042446048675 26.52883263793208 24.13182176098851 104-105 23.244654298665846 26.15792489387966 26.545872463433128 24.05154834402137 106-107 23.034522995604036 26.200395734092314 26.74073568666549 24.024345583638162 108-109 23.032256964049623 26.170526358295138 26.338574892730904 24.458641784924332 110-111 22.87130684330092 26.200395589063586 26.603486363595223 24.324811204040273 112-113 23.030499024952988 26.194597940182728 26.587419004261527 24.18748403060276 114-115 22.94254985410846 26.35129819450952 26.36247242124259 24.343679530139433 116-117 22.66872908313555 26.241540135664167 26.614049170774905 24.47568161042538 118-119 22.776676986871504 26.330516221426524 26.272521636575156 24.620285155126812 120-121 22.63200208448391 26.276193769976086 26.60479239838707 24.487011747152938 122-123 22.792506296681168 26.25328758992461 26.43546054108813 24.51874557230609 124-125 22.685289063621454 26.239650417188702 26.293323308354505 24.781737210835338 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 313.0 1 248.5 2 306.5 3 430.0 4 341.0 5 272.5 6 453.0 7 1054.0 8 1770.0 9 2018.5 10 1630.0 11 1138.5 12 1056.5 13 1159.5 14 1310.5 15 1485.5 16 1702.0 17 1959.0 18 2223.5 19 2531.5 20 2924.5 21 3433.5 22 4201.0 23 5170.5 24 6308.0 25 7936.0 26 10416.0 27 13461.0 28 16862.0 29 21245.5 30 26892.0 31 32611.0 32 38259.5 33 43905.0 34 49442.5 35 54429.0 36 60485.0 37 67312.5 38 71807.5 39 74091.0 40 75753.5 41 79667.5 42 84556.0 43 89399.5 44 93867.5 45 97378.0 46 98899.5 47 101217.0 48 108514.5 49 112629.5 50 112000.0 51 110857.0 52 110565.5 53 112652.0 54 111648.5 55 104565.0 56 96640.0 57 89357.5 58 83294.5 59 76687.0 60 66777.5 61 54030.5 62 41270.0 63 31277.5 64 23197.5 65 16995.0 66 12810.5 67 10295.5 68 7867.0 69 5830.0 70 4376.0 71 3355.0 72 2637.5 73 2132.0 74 1697.5 75 1228.0 76 840.0 77 521.5 78 334.0 79 200.5 80 144.5 81 110.5 82 64.5 83 40.5 84 23.0 85 14.0 86 9.0 87 3.5 88 2.0 89 3.0 90 3.0 91 1.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09938447764145143 2 0.0 3 0.0 4 0.09945409899181322 5 0.0 6 0.0 7 0.0027500433392906 8 0.006370353558103542 9 0.004281713047249922 10-11 8.180508667510013E-4 12-13 0.0 14-15 3.481067518089367E-5 16-17 2.7848540144714937E-4 18-19 0.002262693886758089 20-21 0.0016709124086828962 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 1.7405337590446836E-5 52-53 0.0 54-55 0.0 56-57 0.007936833941243757 58-59 9.224828922936824E-4 60-61 0.0 62-63 0.0 64-65 2.7848540144714937E-4 66-67 0.0 68-69 1.7405337590446836E-5 70-71 0.0 72-73 0.0 74-75 0.0012009682937408317 76-77 0.0 78-79 0.0 80-81 1.2183736313312786E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0015838857207306622 90-91 0.0 92-93 0.0024715579378434506 94-95 0.0 96-97 0.0090681808846228 98-99 0.004142470346526347 100-101 0.0021930725363963014 102-103 0.0 104-105 0.0 106-107 0.001601291058321109 108-109 0.0 110-111 1.3924270072357468E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 9.921042426554697E-4 122-123 0.0047690624997824334 124-125 0.08765328010549027 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2872682.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.41819937466076 #Duplication Level Percentage of deduplicated Percentage of total 1 72.127951204208 32.037937170743135 2 12.592643305157575 11.186850819649525 3 5.373790870646228 7.160823428702878 4 2.8405390444307517 5.046865184281339 5 1.691356820420973 3.7563512231575538 6 1.1026394291538062 2.9386354801509538 7 0.8142083662235652 2.5315968680404586 8 0.5478035789952547 1.946595887197116 9 0.42993959638369333 1.718742844010886 >10 2.2173556404965513 17.755804247680548 >50 0.17110312033787203 5.2093280955049694 >100 0.08632893170978931 6.865538053134087 >500 0.003550984229440572 1.0609858693882988 >1k 7.101968458752774E-4 0.5904296983678419 >5k 7.891076065280859E-5 0.1935151299904337 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5521 0.19218973767371397 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4704 0.16374941605092386 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3024 0.10526748174702247 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 3.481067518089367E-5 7 0.0 0.0 0.0 3.481067518089367E-5 3.481067518089367E-5 8 0.0 0.0 0.0 3.481067518089367E-5 3.481067518089367E-5 9 0.0 0.0 0.0 3.481067518089367E-5 3.481067518089367E-5 10-11 0.0 0.0 0.0 3.481067518089367E-5 3.481067518089367E-5 12-13 0.0 0.0 0.0 3.481067518089367E-5 3.481067518089367E-5 14-15 0.0 0.0 0.0 3.481067518089367E-5 3.481067518089367E-5 16-17 1.7405337590446836E-5 0.0 0.0 3.481067518089367E-5 3.481067518089367E-5 18-19 6.962135036178734E-5 0.0 0.0 3.481067518089367E-5 8.702668795223419E-5 20-21 1.0443202554268103E-4 0.0 0.0 3.481067518089367E-5 1.0443202554268103E-4 22-23 1.0443202554268103E-4 0.0 0.0 8.702668795223418E-5 1.2183736313312786E-4 24-25 1.3924270072357468E-4 0.0 0.0 2.9589073903759624E-4 1.3924270072357468E-4 26-27 1.3924270072357468E-4 0.0 0.0 4.6994411494206457E-4 1.7405337590446836E-4 28-29 1.3924270072357468E-4 0.0 0.0 0.0010443202554268102 1.7405337590446836E-4 30-31 1.3924270072357468E-4 0.0 0.0 0.0032722034670040053 1.7405337590446836E-4 32-33 1.3924270072357468E-4 0.0 0.0 0.007014351048950075 1.7405337590446836E-4 34-35 1.7405337590446836E-4 0.0 0.0 0.012758112453797533 2.7848540144714937E-4 36-37 1.7405337590446836E-4 0.0 0.0 0.02095602645889799 2.7848540144714937E-4 38-39 1.7405337590446836E-4 0.0 0.0 0.035994238137044055 2.7848540144714937E-4 40-41 1.7405337590446836E-4 0.0 0.0 0.05420022125665145 3.1329607662804307E-4 42-43 1.914587134949152E-4 0.0 0.0 0.0723191776883066 3.1329607662804307E-4 44-45 2.4367472626625572E-4 0.0 0.0 0.09165650775129305 3.307014142184899E-4 46-47 2.4367472626625572E-4 0.0 0.0 0.11604138571550907 3.481067518089367E-4 48-49 3.1329607662804307E-4 0.0 0.0 0.14599597170866807 4.5253877735161775E-4 50-51 3.1329607662804307E-4 0.0 0.0 0.1785613583403941 4.5253877735161775E-4 52-53 3.1329607662804307E-4 0.0 0.0 0.21022166741741688 5.221601277134051E-4 54-55 3.1329607662804307E-4 0.0 0.0 0.24644217494313678 6.091868156656393E-4 56-57 3.1329607662804307E-4 0.0 0.0 0.2897466548681685 6.265921532560861E-4 58-59 3.1329607662804307E-4 0.0 0.0 0.33997845915419805 6.265921532560861E-4 60-61 3.1329607662804307E-4 0.0 0.0 0.39534483802940945 6.265921532560861E-4 62-63 3.1329607662804307E-4 0.0 0.0 0.45224288661258016 6.265921532560861E-4 64-65 3.1329607662804307E-4 0.0 0.0 0.5125697867010689 6.265921532560861E-4 66-67 3.829174269898304E-4 0.0 0.0 0.5862465807214303 6.43997490846533E-4 68-69 3.829174269898304E-4 0.0 0.0 0.6662241069495336 6.614028284369798E-4 70-71 4.1772810217072405E-4 0.0 0.0 0.7570799691716661 7.136188412083203E-4 72-73 4.5253877735161775E-4 0.0 0.0 0.8511210081728504 8.702668795223419E-4 74-75 4.5253877735161775E-4 0.0 0.0 0.949304517520561 8.702668795223419E-4 76-77 4.5253877735161775E-4 0.0 0.0 1.0588711176524237 8.702668795223419E-4 78-79 4.5253877735161775E-4 0.0 0.0 1.1884886666884813 8.702668795223419E-4 80-81 6.265921532560861E-4 0.0 0.0 1.331473514994002 9.398882298841291E-4 82-83 6.265921532560861E-4 0.0 0.0 1.4875645825051294 0.0011139416057885975 84-85 6.265921532560861E-4 0.0 0.0 1.649556059459418 0.0011139416057885975 86-87 6.43997490846533E-4 0.0 0.0 1.8181093486853053 0.0011139416057885975 88-89 6.614028284369798E-4 0.0 0.0 2.0069224508664725 0.0011139416057885975 90-91 6.962135036178734E-4 0.0 0.0 2.219963782973542 0.0011139416057885975 92-93 6.962135036178734E-4 0.0 0.0 2.460401116447974 0.0011139416057885975 94-95 6.962135036178734E-4 0.0 0.0 2.7088971212267836 0.0011487522809694912 96-97 6.962135036178734E-4 0.0 0.0 2.9614137589889866 0.0011487522809694912 98-99 7.310241787987672E-4 0.0 0.0 3.2375842505366066 0.0011661576185599382 100-101 8.180508667510013E-4 0.0 0.0 3.552812319637189 0.001183562956150385 102-103 8.702668795223419E-4 0.0 0.0 3.893365154931872 0.001287994981693066 104-105 9.921042426554697E-4 0.0 0.0 4.236615817553075 0.0014272376824166406 106-107 0.0010269149178363632 1.7405337590446836E-5 0.0 4.591371408321562 0.0015142643703688749 108-109 0.0010443202554268102 3.481067518089367E-5 0.0 4.964576656935923 0.001618696395911556 110-111 0.0010617255930172572 3.481067518089367E-5 0.0 5.376073648249267 0.0016709124086828962 112-113 0.001079130930607704 3.481067518089367E-5 0.0 5.822189856030009 0.00170572308386379 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5785 0.0 46.276134 1 GTATCAA 9155 0.0 43.569427 1 ATCAACG 11440 0.0 34.62347 3 TCAACGC 11650 0.0 33.999355 4 CAACGCA 11700 0.0 33.664963 5 AACGCAG 11885 0.0 33.191 6 TATCAAC 11870 0.0 33.068127 2 GTACATG 10835 0.0 31.531149 1 TACATGG 11055 0.0 29.902615 2 ACGCAGA 13840 0.0 28.201597 7 CGCAGAG 14125 0.0 27.548328 8 ACATGGG 11685 0.0 27.5258 3 GAGTACT 7920 0.0 24.677923 12-13 GCAGAGT 15180 0.0 24.613804 9 CATGGGG 6475 0.0 23.365168 4 AGAGTAC 13370 0.0 23.250612 10-11 GTACTTT 8695 0.0 22.649406 14-15 AGTACTT 8670 0.0 22.47453 12-13 CAGAGTA 14965 0.0 21.011051 10-11 CATGGGA 6630 0.0 20.213612 4 >>END_MODULE