FastQCFastQC Report
Thu 26 May 2016
SRR1364584_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364584_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2139989
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38910.18182336451262132No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26690.12472026725371017No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29900.056.3165861
GTATCAA58300.053.4790651
ATCAACG69900.043.49453
TCAACGC70000.043.4323654
CAACGCA71900.042.6156355
AACGCAG72800.042.0848586
TATCAAC74300.041.0789342
GTACATG90100.038.896591
TACATGG91900.036.8372652
ACGCAGA86250.035.108217
CGCAGAG85150.034.5153778
ACATGGG96850.033.4801563
GCAGAGT93400.029.4927049
CATGGGA60900.027.0615774
CATGGGG42200.026.3645134
GTATAGG8600.026.2909471
GAGTACT45600.024.92675212-13
CAGAGTA92850.023.67957510-11
AGAGTAC86100.023.63547510-11
GTGTAGC12150.022.5269621
Produced by FastQC (version 0.11.5)