FastQCFastQC Report
Thu 26 May 2016
SRR1364584_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364584_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2139989
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58930.27537524725594387No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51400.24018815049983902No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34540.16140269879891905No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40250.064.4188841
GTATCAA73650.050.9867861
ATCAACG94450.040.042033
TCAACGC96650.039.068954
CAACGCA97100.038.7866335
TATCAAC96900.038.477342
AACGCAG100950.037.6610266
GTACATG97850.032.8944631
TACATGG99900.031.5983832
ACGCAGA121100.030.9523757
CGCAGAG120500.030.8102448
TGGTATC20900.030.4924242
ACATGGG100350.030.090883
GCAGAGT132050.026.8982099
GTGGTAT24750.026.4915471
GAGTACT77950.025.95137812-13
CATGGGG43050.024.3491524
CGCGGAA3750.023.79838482-83
CATGGGA62200.023.746834
AGAGTAC123650.023.48081410-11
Produced by FastQC (version 0.11.5)