##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364584_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2139989 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.02101973421359 31.0 30.0 34.0 26.0 34.0 2 29.946051591853976 31.0 30.0 34.0 26.0 34.0 3 30.209099205650123 31.0 30.0 34.0 26.0 34.0 4 33.73774257718147 35.0 35.0 37.0 28.0 37.0 5 33.16842376292588 35.0 33.0 37.0 26.0 37.0 6 33.1894874225989 35.0 33.0 37.0 26.0 37.0 7 32.9707031204366 35.0 33.0 37.0 26.0 37.0 8 32.90197566436089 35.0 33.0 37.0 26.0 37.0 9 34.14259886382594 38.0 34.0 39.0 25.0 39.0 10-11 34.07416486720259 38.0 34.0 39.0 25.0 39.0 12-13 33.95078899938271 38.0 33.5 39.0 24.5 39.0 14-15 34.911158188196296 39.0 34.0 41.0 24.5 41.0 16-17 34.91947108139341 39.0 34.0 41.0 25.0 41.0 18-19 34.93505130166557 39.0 34.0 41.0 24.5 41.0 20-21 34.95663272100931 39.0 34.0 41.0 24.0 41.0 22-23 34.91534746206639 39.0 34.0 41.0 24.0 41.0 24-25 34.874506597931116 39.0 34.0 41.0 24.0 41.0 26-27 34.75860927322523 39.0 34.0 41.0 24.0 41.0 28-29 34.6137015190265 39.0 33.5 41.0 23.5 41.0 30-31 34.458250719980335 38.5 33.0 41.0 23.0 41.0 32-33 34.27158924648678 38.0 33.0 40.0 22.5 41.0 34-35 34.09286215957185 38.0 33.0 40.0 21.0 41.0 36-37 33.89507282514069 38.0 33.0 40.0 18.5 41.0 38-39 33.73197245406402 38.0 33.0 40.0 18.0 41.0 40-41 33.773659116939385 38.0 33.0 40.0 18.0 41.0 42-43 33.652702654079064 38.0 33.0 40.0 15.5 41.0 44-45 33.5001656550571 38.0 33.0 40.0 15.0 41.0 46-47 33.26921026229574 38.0 32.0 40.0 11.0 41.0 48-49 33.00378670170735 38.0 32.0 40.0 8.5 41.0 50-51 32.837872063828364 38.0 32.0 40.0 7.5 41.0 52-53 32.66040269365871 38.0 31.0 40.0 4.5 41.0 54-55 32.47410524072787 38.0 31.0 40.0 2.0 41.0 56-57 32.26468967831143 37.0 31.0 40.0 2.0 41.0 58-59 32.06657020199637 37.0 31.0 40.0 2.0 41.0 60-61 31.83343746159443 36.5 31.0 40.0 2.0 41.0 62-63 31.663338456412625 36.0 30.5 40.0 2.0 41.0 64-65 31.526942895500866 36.0 30.5 39.5 2.0 41.0 66-67 31.23597434379336 35.5 30.0 39.0 2.0 41.0 68-69 30.949847873049816 35.0 30.0 39.0 2.0 41.0 70-71 30.635573360423816 35.0 30.0 38.5 2.0 40.0 72-73 30.34219731970585 35.0 30.0 37.5 2.0 40.0 74-75 29.98647609870892 35.0 29.0 37.0 2.0 39.0 76-77 29.674760477740776 35.0 29.0 36.5 2.0 39.0 78-79 29.381969019466922 34.5 29.0 36.0 2.0 39.0 80-81 29.094721514923677 34.0 29.0 36.0 2.0 37.5 82-83 28.801379820176646 34.0 29.0 35.0 2.0 37.0 84-85 28.57369687414281 34.0 29.0 35.0 2.0 37.0 86-87 28.352465596785777 34.0 29.0 35.0 2.0 36.0 88-89 28.172439905064934 34.0 28.0 35.0 2.0 36.0 90-91 28.028753886118107 34.0 28.0 35.0 2.0 36.0 92-93 27.913858435720933 34.0 28.0 35.0 2.0 35.0 94-95 27.79709451777556 34.0 27.0 35.0 2.0 35.0 96-97 27.678900218645985 34.0 27.0 35.0 2.0 35.0 98-99 27.593049543712606 34.0 27.0 35.0 2.0 35.0 100-101 27.490917476678618 34.0 27.0 35.0 2.0 35.0 102-103 26.223330587213297 31.5 24.5 34.0 2.0 35.0 104-105 27.159801522344274 33.5 26.5 35.0 2.0 35.0 106-107 27.322136702571836 34.0 27.0 35.0 2.0 35.0 108-109 27.325068493342723 34.0 27.0 35.0 2.0 35.0 110-111 27.246852437091967 34.0 27.0 35.0 2.0 35.0 112-113 27.163060417600278 34.0 27.0 35.0 2.0 35.0 114-115 27.06202087954658 34.0 26.0 35.0 2.0 35.0 116-117 26.939184500481076 34.0 25.5 35.0 2.0 35.0 118-119 26.802534265363047 34.0 25.0 35.0 2.0 35.0 120-121 26.61083070987748 34.0 25.0 35.0 2.0 35.0 122-123 26.319292061781624 34.0 25.0 35.0 2.0 35.0 124-125 24.52214333811996 31.5 19.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.782721362370129 1101 2 0.963571198050829 1101 3 0.8169374706829196 1101 4 0.7853312113794075 1101 5 0.8832716080453835 1101 6 0.8549225761810746 1101 7 0.8958466841500083 1101 8 1.0760787126216087 1101 9 1.1619979166986099 1101 10-11 1.2327976311565436 1101 12-13 1.105848577805638 1101 14-15 1.2694298132227644 1101 16-17 1.301219912438846 1101 18-19 1.4657150748598724 1101 20-21 1.3865728840768128 1101 22-23 1.3454638209076109 1101 24-25 1.2936593934609633 1101 26-27 1.250334225025199 1101 28-29 1.4166139550912291 1101 30-31 1.463424926019492 1101 32-33 1.5571243217251904 1101 34-35 1.5142139522064682 1101 36-37 1.5962035288568899 1101 38-39 1.5278159056117957 1101 40-41 1.2173594010749795 1101 42-43 1.2688184659311688 1101 44-45 1.3390934224966955 1101 46-47 1.4030680848433903 1101 48-49 1.39226240978374 1101 50-51 1.467028273080878 1101 52-53 1.542249873639392 1101 54-55 1.5278932679237514 1101 56-57 1.5555044968879912 1101 58-59 1.4844858891045405 1101 60-61 1.4770454229794012 1101 62-63 1.4334939965802391 1101 64-65 1.376920906089488 1101 66-67 1.474567374435967 1101 68-69 1.5226005109601886 1101 70-71 1.4783749742151215 1101 72-73 1.423022770922124 1101 74-75 1.4673476301939985 1101 76-77 1.4188096823834186 1101 78-79 1.4170228173705617 1101 80-81 1.3387586299433636 1101 82-83 1.4463949795094386 1101 84-85 1.4084611089262324 1101 86-87 1.4002240592317712 1101 88-89 1.43448520175939 1101 90-91 1.4311612212248086 1101 92-93 1.4762342164812345 1101 94-95 1.5120499245692294 1101 96-97 1.4657308483244265 1101 98-99 1.378400246797085 1101 100-101 1.4546683792794894 1101 102-103 1.3663670732898687 1101 104-105 1.4096009104621245 1101 106-107 1.3982446444273293 1101 108-109 1.3559889686204585 1101 110-111 1.231137094377182 1101 112-113 1.281981635025062 1101 114-115 1.332192321126957 1101 116-117 1.380299030367091 1101 118-119 1.4073431491433048 1101 120-121 1.307628731728407 1101 122-123 1.2875239212829683 1101 124-125 1.5686192250652482 1102 1 0.23635505806820234 1102 2 -0.5163190837866765 1102 3 0.03130878271852566 1102 4 -0.044029325114337325 1102 5 0.17668734534110797 1102 6 0.14918499112232553 1102 7 0.11490179893533536 1102 8 0.22102910446660928 1102 9 0.560366102419529 1102 10-11 0.5717497097100903 1102 12-13 0.5798757489052875 1102 14-15 0.7752719085261006 1102 16-17 0.8862807144894802 1102 18-19 0.8478244497376934 1102 20-21 0.6128068712931736 1102 22-23 0.8289128466516189 1102 24-25 0.7594900563679232 1102 26-27 0.9239316842493892 1102 28-29 0.5805785256100364 1102 30-31 0.7140269449087526 1102 32-33 0.7545804571373083 1102 34-35 0.7955468898631537 1102 36-37 0.5095332377608628 1102 38-39 -0.07800775475688937 1102 40-41 -0.509940019798286 1102 42-43 -0.07528170985560934 1102 44-45 -0.29972701146517977 1102 46-47 -0.03646269035440497 1102 48-49 -0.13710504714146055 1102 50-51 -0.3510399373502793 1102 52-53 -0.5682834922238769 1102 54-55 -0.41388977537703653 1102 56-57 -0.28609386472918885 1102 58-59 -0.2603317047435496 1102 60-61 -0.3854145206249484 1102 62-63 -0.371520892122021 1102 64-65 -0.31941065921045464 1102 66-67 -0.4036764311913963 1102 68-69 -0.2985696753519065 1102 70-71 -0.776759047564294 1102 72-73 -0.706134375263975 1102 74-75 -0.37171177369489783 1102 76-77 -0.742857265792324 1102 78-79 -0.44957198628467765 1102 80-81 -0.5078165775522834 1102 82-83 -0.8491981522946794 1102 84-85 -0.7266067504005527 1102 86-87 -0.45509024565194167 1102 88-89 -0.6259560676275946 1102 90-91 -0.6125276189734343 1102 92-93 -0.5084449452785726 1102 94-95 -0.25706923353095945 1102 96-97 -0.473842055488614 1102 98-99 -0.5938652444127968 1102 100-101 -0.35155406606474315 1102 102-103 -0.4423140801466552 1102 104-105 -0.37233316636795877 1102 106-107 -0.2347732814450758 1102 108-109 -0.27521381018691216 1102 110-111 -0.20451628427911217 1102 112-113 -0.06207455496854308 1102 114-115 -0.037693206335035256 1102 116-117 -0.05988508047867214 1102 118-119 0.16328514107025072 1102 120-121 0.372839820527318 1102 122-123 0.3256961610473752 1102 124-125 0.5705968263264083 1103 1 0.21157187841435743 1103 2 -0.3193975548017143 1103 3 0.188904265549251 1103 4 0.24365364165562653 1103 5 0.3290945707964781 1103 6 0.3001058083948891 1103 7 0.4288732485612101 1103 8 0.3097868829162067 1103 9 0.6829428161977802 1103 10-11 0.7015888028751291 1103 12-13 0.6936481709948268 1103 14-15 0.7082520624575182 1103 16-17 0.5280720882833521 1103 18-19 0.728886601754418 1103 20-21 0.8123327637679196 1103 22-23 0.7843229630449358 1103 24-25 0.6583296659968241 1103 26-27 0.7172457604089146 1103 28-29 0.75804369579118 1103 30-31 0.610912100981615 1103 32-33 0.6503051441098222 1103 34-35 0.8234658554238266 1103 36-37 0.6709565365792969 1103 38-39 0.7718004607974933 1103 40-41 0.7132672365189521 1103 42-43 0.7168474911370026 1103 44-45 0.7153649915328231 1103 46-47 0.5910393251143162 1103 48-49 0.552947355045319 1103 50-51 0.5071887648138258 1103 52-53 0.49506442176599563 1103 54-55 0.6775550322716626 1103 56-57 0.6087541720783065 1103 58-59 0.5174557829746931 1103 60-61 0.62626732504053 1103 62-63 0.5769366444713384 1103 64-65 0.5495838738306347 1103 66-67 0.4720179607668875 1103 68-69 0.5616933905539199 1103 70-71 0.5015716719580396 1103 72-73 0.4562486330750275 1103 74-75 0.5743090927047234 1103 76-77 0.6439470038521975 1103 78-79 0.5004959570554561 1103 80-81 0.5108729583860452 1103 82-83 0.5512931969356671 1103 84-85 0.6255617679780023 1103 86-87 0.5863559791232049 1103 88-89 0.6556159349925146 1103 90-91 0.4906069072654482 1103 92-93 0.5389921815447956 1103 94-95 0.45000718737448864 1103 96-97 0.4882545358499115 1103 98-99 0.5570634457844683 1103 100-101 0.46319168570509106 1103 102-103 0.26386128452721636 1103 104-105 0.25679145888082644 1103 106-107 0.4344013053838367 1103 108-109 0.3954381309614021 1103 110-111 0.3604876994207835 1103 112-113 0.2815353069718505 1103 114-115 0.432135384348463 1103 116-117 0.5422149434550185 1103 118-119 0.3453803782657232 1103 120-121 0.24061286930166403 1103 122-123 0.34779703525693506 1103 124-125 0.6644247682809095 1104 1 0.4439590628014898 1104 2 0.6056820067258677 1104 3 0.2981051894410349 1104 4 0.13210198497019832 1104 5 0.3947886934002298 1104 6 0.5445887901277118 1104 7 0.5820762113346944 1104 8 0.44186466136274305 1104 9 0.5733687242901127 1104 10-11 0.5794570617754857 1104 12-13 0.6226535202379537 1104 14-15 0.7534330414216797 1104 16-17 0.6185077769889986 1104 18-19 0.621040668984854 1104 20-21 0.5142322717278347 1104 22-23 0.5698470910656397 1104 24-25 0.6896926132717951 1104 26-27 0.5150055297369178 1104 28-29 0.7321288322683515 1104 30-31 0.6141374463124976 1104 32-33 0.7934910660454833 1104 34-35 0.883900641303633 1104 36-37 0.8870896582501331 1104 38-39 1.0344324990773117 1104 40-41 0.9008649486077829 1104 42-43 0.8638298897948076 1104 44-45 0.9119595512547534 1104 46-47 0.7783260325084527 1104 48-49 0.8899549387824237 1104 50-51 0.667708660838386 1104 52-53 0.6769020109200525 1104 54-55 0.26103162651163814 1104 56-57 0.5397721646450435 1104 58-59 0.5497233429373694 1104 60-61 0.40812767292942453 1104 62-63 0.49380293596370706 1104 64-65 0.542744591878126 1104 66-67 0.6016415576250544 1104 68-69 0.3016066628461971 1104 70-71 -0.12031226225604996 1104 72-73 -0.07310731353787148 1104 74-75 0.3370052692372667 1104 76-77 0.5129907043848618 1104 78-79 0.5709014199041746 1104 80-81 0.6406949888413678 1104 82-83 0.5239976681806624 1104 84-85 0.5421707541045784 1104 86-87 0.6580463700467973 1104 88-89 0.6743856843078753 1104 90-91 0.6689392750766956 1104 92-93 0.5956592682762611 1104 94-95 0.608081930069126 1104 96-97 0.7414053980485917 1104 98-99 0.7217986921054518 1104 100-101 0.6797208281710105 1104 102-103 0.65687209586671 1104 104-105 0.7225835757064871 1104 106-107 0.700650007571685 1104 108-109 0.6468153165523027 1104 110-111 0.5857212115637935 1104 112-113 0.6833208331688461 1104 114-115 0.6029912256316763 1104 116-117 0.5069605346554766 1104 118-119 0.5330062096178594 1104 120-121 0.5435838246418783 1104 122-123 0.6009268315192386 1104 124-125 0.9508910326885065 1105 1 0.3384903460837947 1105 2 0.033339358514844264 1105 3 0.301848181055977 1105 4 0.3369301996587737 1105 5 0.03427162415081142 1105 6 0.4645106629571316 1105 7 0.6145773365659934 1105 8 0.6774075229509577 1105 9 0.776320812589745 1105 10-11 0.8043348607304566 1105 12-13 0.8069799280736376 1105 14-15 0.8766169736744231 1105 16-17 0.9373035850162097 1105 18-19 0.7748811806455791 1105 20-21 0.8614694815987534 1105 22-23 0.7725593152450543 1105 24-25 0.7897371594655453 1105 26-27 0.8925198891700532 1105 28-29 0.9522902913830933 1105 30-31 0.9291609048382341 1105 32-33 1.085495441018864 1105 34-35 1.087962494758692 1105 36-37 1.2263784750254274 1105 38-39 1.156595110280719 1105 40-41 0.8732139870212379 1105 42-43 0.9242830570199132 1105 44-45 0.9209576971699391 1105 46-47 0.924927741378248 1105 48-49 0.979399620930046 1105 50-51 0.8573617191522231 1105 52-53 0.8559318670571443 1105 54-55 0.8880285647711688 1105 56-57 0.8587303208586405 1105 58-59 0.55376676667818 1105 60-61 0.5311562028679901 1105 62-63 0.7509973777841523 1105 64-65 0.4693076446537887 1105 66-67 0.7238570500688688 1105 68-69 0.7755124101386954 1105 70-71 0.7127081363814476 1105 72-73 0.602558425160268 1105 74-75 0.6758321392049851 1105 76-77 0.8264875528111162 1105 78-79 0.7107740603913868 1105 80-81 0.6500896051421279 1105 82-83 0.823965902391766 1105 84-85 0.7802307997806324 1105 86-87 0.837039274143752 1105 88-89 0.7112780825601028 1105 90-91 0.6975647888397098 1105 92-93 0.7272958639468534 1105 94-95 0.8067928687991213 1105 96-97 0.8167459773158114 1105 98-99 0.7339580433795092 1105 100-101 0.698860873624799 1105 102-103 0.33100011106983374 1105 104-105 0.6194320019570689 1105 106-107 0.6208357309333401 1105 108-109 0.6028313679999115 1105 110-111 0.5677567781339548 1105 112-113 0.5581628840348607 1105 114-115 0.5609823126884592 1105 116-117 0.4025589817436739 1105 118-119 0.5259706893535068 1105 120-121 0.5585105040148619 1105 122-123 0.5307587043671731 1105 124-125 0.9356623467919611 1106 1 -0.24755542504701822 1106 2 -0.7720389767940077 1106 3 -0.09587784853507486 1106 4 -0.3807057868843913 1106 5 0.027021226509553742 1106 6 0.20686563150859882 1106 7 0.2927523859351595 1106 8 0.1621947139213944 1106 9 0.22158973687380978 1106 10-11 0.23269383445324365 1106 12-13 0.2792715409351487 1106 14-15 0.2988488322671401 1106 16-17 0.31274807670267535 1106 18-19 0.24193127836890227 1106 20-21 0.25352750207272123 1106 22-23 0.18607445643918652 1106 24-25 0.17475652556279897 1106 26-27 0.3448984248502498 1106 28-29 0.42087635012385505 1106 30-31 0.37645760216960866 1106 32-33 0.3609340493815978 1106 34-35 0.31831952416516884 1106 36-37 0.3598766354059677 1106 38-39 0.2220099635491266 1106 40-41 0.1880325193782042 1106 42-43 -0.018050502248080136 1106 44-45 0.047274631255284305 1106 46-47 -0.0511369992423667 1106 48-49 0.2344474375026735 1106 50-51 0.27062291880140066 1106 52-53 0.18305744801186563 1106 54-55 0.17923758215836472 1106 56-57 -0.30309519742834823 1106 58-59 0.2335984509891773 1106 60-61 0.2533398294840623 1106 62-63 0.1358982315615691 1106 64-65 0.22500234488810378 1106 66-67 0.018873968853814205 1106 68-69 0.0740162790349359 1106 70-71 0.273530331812303 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-1.7381736500416949 2216 62-63 -1.90848082766637 2216 64-65 -1.7803703333897474 2216 66-67 -1.5604673866642926 2216 68-69 -1.402394245395584 2216 70-71 -1.581241782350041 2216 72-73 -1.389835131710619 2216 74-75 -1.305158995233107 2216 76-77 -1.4216012662761734 2216 78-79 -1.458779164535649 2216 80-81 -1.3176130152113181 2216 82-83 -1.2793045991554877 2216 84-85 -1.2891955821203354 2216 86-87 -1.4161949126382964 2216 88-89 -1.3896963636397253 2216 90-91 -1.3201502985485156 2216 92-93 -1.3489374672137977 2216 94-95 -1.25621169692905 2216 96-97 -1.3883108796050507 2216 98-99 -1.4187398539852367 2216 100-101 -1.4337306970043144 2216 102-103 -1.4077539510625527 2216 104-105 -1.3516734463957825 2216 106-107 -1.2905104605168596 2216 108-109 -1.322882693056485 2216 110-111 -1.462875916121586 2216 112-113 -1.3892779596264582 2216 114-115 -1.4342515037036705 2216 116-117 -1.6168510533399072 2216 118-119 -1.6620241769181447 2216 120-121 -1.69521796707145 2216 122-123 -1.6605616529039011 2216 124-125 -2.0205558583895673 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 67868.0 3 22851.0 4 10990.0 5 8448.0 6 9150.0 7 10824.0 8 12076.0 9 13167.0 10 14190.0 11 15608.0 12 17100.0 13 19187.0 14 22403.0 15 20223.0 16 13953.0 17 12336.0 18 11487.0 19 10648.0 20 10667.0 21 11134.0 22 12528.0 23 15476.0 24 19971.0 25 26533.0 26 33652.0 27 37575.0 28 42887.0 29 51370.0 30 59523.0 31 69682.0 32 84443.0 33 105104.0 34 134788.0 35 184965.0 36 294378.0 37 457817.0 38 174368.0 39 619.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.8307757359407 19.35710196338164 13.656578469782183 24.155543830895475 2 15.369198331953262 20.440441722103113 39.78489599007097 24.40546395587265 3 19.37832390727242 25.135736679020315 29.180664012758946 26.305275400948325 4 12.579543349605302 17.002490378084673 36.545456416512465 33.87250985579757 5 13.03464643977142 38.77800306450173 34.109287477645914 14.078063018080933 6 31.9571268824279 37.187293953380134 17.118499207238916 13.73707995695305 7 28.339200290100102 32.79430229944517 20.508787350738405 18.35771005971632 8 26.175151516001183 36.385710740777604 19.032905223814684 18.406232519406526 9 26.388300860126417 16.00005233845409 19.50345760912334 38.10818919229615 10-11 25.099609692157976 27.179468008425324 27.014162532082697 20.706759767334002 12-13 25.826534622374226 24.230428287248206 27.86140489507189 22.081632195305676 14-15 22.607585324680635 25.46007862195457 25.881506317209794 26.050829736155002 16-17 22.21050354138327 29.075067418759872 26.31256883816367 22.40186020169319 18-19 21.94768854628515 27.24843828272784 29.101937800474033 21.701935370512984 20-21 22.76780019093788 26.379194406632262 29.2445243212149 21.608481081214954 22-23 22.394788010592578 26.446818184579453 29.06916811254637 22.089225692281598 24-25 22.17787100774817 26.546211218842714 29.184355620519543 22.091562152889573 26-27 22.07485645954255 26.86004460770593 28.989050878298904 22.076048054452617 28-29 21.886187265448562 26.807474244026487 29.247369963116633 22.058968527408318 30-31 22.036888974662954 26.573197338864823 29.262650415492793 22.12726327097943 32-33 21.919622016748686 26.739483240334412 29.110430006883213 22.23046473603369 34-35 22.34301204351985 26.663221166090107 28.963676916096297 22.030089874293747 36-37 22.286422967594692 26.524201759915588 28.88376996330355 22.30560530918617 38-39 22.116468822970585 26.751399189435087 29.0329062439106 22.09922574368373 40-41 22.249506889988687 26.568617876073198 29.04615397555782 22.1357212583803 42-43 22.172847617441025 26.609038644591166 28.811152767607684 22.406960970360128 44-45 22.27361916346299 26.785394691281123 28.57495996474748 22.366026180508406 46-47 22.31235768034322 27.018877199836073 28.136756777721754 22.532008342098955 48-49 22.58198523450354 27.070489614666243 27.848414174091552 22.499110976738663 50-51 22.488853914669654 27.215934287512695 27.708133079188723 22.587078718628927 52-53 22.724462602377862 27.158807825647703 27.64074955525472 22.475980016719713 54-55 22.535606491435235 27.032872598877844 27.75981558783713 22.671705321849785 56-57 22.492060736330927 27.4543721620243 27.590994558236726 22.462572543408047 58-59 22.5176152882267 27.134471524997856 27.708216947546315 22.63969623922913 60-61 22.630396698300785 27.093036459533202 27.891802247581648 22.384764594584365 62-63 22.459554698645647 27.13184507023167 27.943695037684773 22.464905193437907 64-65 22.319676221878296 26.95073888402904 27.86686722891386 22.862717665178806 66-67 22.318222196469232 27.4177343902235 27.60815592977347 22.655887483533792 68-69 22.33399852382384 27.125542964366396 27.830803763665084 22.709654748144676 70-71 22.186796287270635 27.15341060164328 27.826474808982667 22.83331830210342 72-73 22.420325525037747 27.134088072415324 27.690375978568117 22.755210423978813 74-75 22.379922101529697 27.231099574058454 27.57404910827981 22.81492921613204 76-77 22.44983502251647 27.138620805994794 27.656637487388952 22.75490668409978 78-79 22.36168036377757 27.28532716756955 27.51366946278696 22.839323005865918 80-81 22.219042036012844 27.484051885374043 27.529566191188586 22.767339887424523 82-83 22.39544221956281 27.446963512429267 27.424533490592708 22.73306077741521 84-85 22.396516991442482 27.239065247531645 27.36920610339586 22.995211657630016 86-87 22.32429699404997 27.100466404266566 27.66640389273029 22.908832708953177 88-89 22.40969699461953 27.18111349640788 27.45062762796723 22.958561881005362 90-91 22.285838852442698 27.412734364522436 27.5355387340776 22.76588804895726 92-93 22.342090672231933 27.300504196732756 27.4802523530466 22.877152777988712 94-95 22.285114549654228 27.08481211819313 27.516800320001643 23.113273012150998 96-97 22.406642591517308 27.03108077637099 27.46261088802719 23.09966574408451 98-99 22.08849933023025 27.03315950240068 27.47217236950355 23.406168797865522 100-101 21.982356580627194 27.110939144315655 27.670602276317 23.236101998740153 102-103 22.38413375022021 26.979320921743057 27.41497736670609 23.221567961330642 104-105 22.189553310788046 26.958736703786794 27.596800731218714 23.254909254206446 106-107 22.101142568352344 26.998384782515743 27.632836041990515 23.267636607141398 108-109 22.10948280575274 27.139368473389347 27.224322181095324 23.526826539762588 110-111 21.948214590289183 27.088507629726376 27.528253702712867 23.435024077271567 112-113 22.089319150705915 27.135489948780112 27.463295372079017 23.31189552843496 114-115 21.918850984748055 27.205887506898403 27.289953359573342 23.585308148780204 116-117 21.807378449141563 27.11310665615571 27.457617772801633 23.621897121901096 118-119 21.8749488899242 27.16656487486618 27.182382713182168 23.77610352202745 120-121 21.668024162966848 27.247240497409074 27.361728137183295 23.723007202440783 122-123 21.827786320470693 27.21372509206598 27.220734747398893 23.737753840064432 124-125 21.69687386588546 27.153718586196447 27.134029422438726 24.015378125479366 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 225.0 1 194.0 2 287.5 3 456.0 4 356.0 5 262.5 6 531.0 7 1324.5 8 2177.0 9 2406.0 10 1899.0 11 1264.0 12 1107.5 13 1190.5 14 1357.0 15 1578.5 16 1803.5 17 2013.5 18 2326.5 19 2699.5 20 3112.5 21 3659.0 22 4366.5 23 5305.5 24 6543.0 25 8407.5 26 11186.5 27 14265.0 28 17102.0 29 20394.5 30 24409.0 31 28868.0 32 33718.5 33 38526.0 34 43296.5 35 47284.5 36 51612.0 37 57087.5 38 60846.0 39 62875.0 40 64822.0 41 67317.5 42 70196.5 43 73501.5 44 76773.5 45 79170.5 46 79114.0 47 78821.5 48 82468.5 49 83910.0 50 81818.5 51 79428.0 52 75995.5 53 74687.5 54 72138.0 55 65992.0 56 59679.0 57 53518.0 58 48541.5 59 43402.0 60 36746.0 61 29351.5 62 23082.5 63 17927.5 64 13594.5 65 10559.5 66 7807.0 67 5969.0 68 4453.5 69 2926.0 70 2032.5 71 1487.0 72 1141.0 73 980.0 74 799.0 75 620.0 76 427.5 77 234.0 78 124.5 79 64.0 80 39.5 81 35.0 82 30.0 83 13.5 84 10.0 85 10.5 86 6.0 87 4.0 88 3.5 89 2.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09556123886618109 2 4.672921215950175E-5 3 0.0 4 0.1009818274766833 5 0.0 6 0.0 7 0.0017757100620610666 8 0.005420588610502203 9 0.0033177740633246245 10-11 5.373859398342701E-4 12-13 0.0 14-15 7.009381823925263E-5 16-17 2.8037527295701054E-4 18-19 0.0017289808499015647 20-21 0.0012149595161470455 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.006986017217845513 58-59 7.243027884722772E-4 60-61 0.0 62-63 0.0 64-65 2.3364606079750877E-4 66-67 0.0 68-69 2.3364606079750876E-5 70-71 0.0 72-73 0.0 74-75 0.0011215010918280421 76-77 0.0 78-79 0.0 80-81 1.1682303039875439E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0012616887283065474 90-91 0.0 92-93 0.002359825214054839 94-95 0.0 96-97 0.007546767763759533 98-99 0.003481326305882881 100-101 0.001915897698539572 102-103 0.0 104-105 0.0 106-107 0.0013785117587053017 108-109 0.0 110-111 1.86916848638007E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 9.345842431900351E-4 122-123 0.0039018892153183963 124-125 0.08210322576424459 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2139989.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.21518666891904 #Duplication Level Percentage of deduplicated Percentage of total 1 79.26079003282894 47.727032673527916 2 12.000347562249598 14.45206337105534 3 3.819701748190902 6.900121613607347 4 1.7125330328467803 4.124819849982357 5 0.9098941185150758 2.739472209766842 6 0.5095458061053454 1.8409437498598917 7 0.33842588597763 1.4264864528416112 8 0.23593503257353224 1.1365497622522192 9 0.1745081813205199 0.9457238442121809 >10 0.9272025925684447 10.207969577504578 >50 0.07028321239397134 2.952899623724776 >100 0.03865259533093041 4.05554863290732 >500 0.0017130135772998405 0.6382605555544654 >1k 3.114570140544914E-4 0.3348136801009424 >5k 1.557285070272457E-4 0.5172944031023243 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5893 0.27537524725594387 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5140 0.24018815049983902 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3454 0.16140269879891905 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 4.672921215950175E-5 0.0 6 0.0 0.0 0.0 4.672921215950175E-5 0.0 7 0.0 0.0 0.0 4.672921215950175E-5 0.0 8 0.0 0.0 0.0 4.672921215950175E-5 0.0 9 0.0 0.0 0.0 4.672921215950175E-5 0.0 10-11 0.0 0.0 0.0 1.1682303039875439E-4 0.0 12-13 0.0 0.0 0.0 1.4018763647850527E-4 0.0 14-15 0.0 0.0 0.0 1.4018763647850527E-4 0.0 16-17 0.0 0.0 0.0 1.6355224255825614E-4 2.3364606079750876E-5 18-19 0.0 0.0 0.0 2.3364606079750877E-4 4.672921215950175E-5 20-21 0.0 0.0 0.0 2.8037527295701054E-4 4.672921215950175E-5 22-23 0.0 0.0 0.0 4.6729212159501754E-4 4.672921215950175E-5 24-25 0.0 0.0 0.0 6.308443641532737E-4 4.672921215950175E-5 26-27 0.0 0.0 0.0 8.177612127912807E-4 4.672921215950175E-5 28-29 0.0 0.0 0.0 0.0011682303039875438 4.672921215950175E-5 30-31 0.0 0.0 0.0 0.0025467420626928455 4.672921215950175E-5 32-33 0.0 0.0 0.0 0.006331808247612487 4.672921215950175E-5 34-35 0.0 0.0 0.0 0.010887906433163909 4.672921215950175E-5 36-37 0.0 0.0 0.0 0.017710371408451164 4.672921215950175E-5 38-39 0.0 0.0 0.0 0.03135530135902568 4.672921215950175E-5 40-41 0.0 0.0 0.0 0.04796753628172855 4.672921215950175E-5 42-43 0.0 0.0 0.0 0.0637853745977199 4.672921215950175E-5 44-45 0.0 0.0 0.0 0.08243033024936108 4.672921215950175E-5 46-47 0.0 0.0 0.0 0.10558465487439421 7.009381823925263E-5 48-49 0.0 0.0 0.0 0.13191656592627346 9.34584243190035E-5 50-51 0.0 0.0 0.0 0.165912067772311 9.34584243190035E-5 52-53 0.0 0.0 0.0 0.19743092137389492 9.34584243190035E-5 54-55 0.0 0.0 0.0 0.23264138273607948 9.34584243190035E-5 56-57 0.0 0.0 0.0 0.2769406758632872 9.34584243190035E-5 58-59 0.0 0.0 0.0 0.33116992657438893 9.34584243190035E-5 60-61 0.0 0.0 0.0 0.38946461874336735 9.34584243190035E-5 62-63 0.0 0.0 0.0 0.4497219378230449 9.34584243190035E-5 64-65 0.0 0.0 0.0 0.5081801822345816 9.34584243190035E-5 66-67 0.0 0.0 0.0 0.5771291347759264 9.34584243190035E-5 68-69 0.0 0.0 0.0 0.6584379639334594 9.34584243190035E-5 70-71 0.0 0.0 0.0 0.7517094714038249 9.34584243190035E-5 72-73 0.0 0.0 0.0 0.8533455078507413 9.34584243190035E-5 74-75 0.0 0.0 0.0 0.9520843331437685 1.1682303039875439E-4 76-77 0.0 0.0 0.0 1.059444698080224 1.4018763647850527E-4 78-79 0.0 0.0 0.0 1.1890248033985222 1.86916848638007E-4 80-81 0.0 0.0 0.0 1.3417358687357739 1.86916848638007E-4 82-83 0.0 0.0 0.0 1.4969002177113995 1.86916848638007E-4 84-85 0.0 0.0 0.0 1.6586066563893551 1.86916848638007E-4 86-87 0.0 0.0 0.0 1.824915922465022 1.86916848638007E-4 88-89 0.0 0.0 0.0 2.018024391714163 1.86916848638007E-4 90-91 0.0 0.0 0.0 2.2425816207466487 1.86916848638007E-4 92-93 0.0 0.0 0.0 2.4847090335511073 1.86916848638007E-4 94-95 0.0 0.0 0.0 2.736065465757067 1.86916848638007E-4 96-97 2.3364606079750876E-5 0.0 0.0 2.986487313719837 2.3364606079750877E-4 98-99 4.672921215950175E-5 0.0 0.0 3.2623999469156146 2.8037527295701054E-4 100-101 4.672921215950175E-5 0.0 0.0 3.5811399030555764 3.037398790367614E-4 102-103 9.34584243190035E-5 0.0 0.0 3.934295923951011 3.2710448511651227E-4 104-105 9.34584243190035E-5 0.0 0.0 4.286470631391095 3.2710448511651227E-4 106-107 9.34584243190035E-5 0.0 0.0 4.6296499654904775 3.2710448511651227E-4 108-109 9.34584243190035E-5 0.0 0.0 4.999044387611338 3.2710448511651227E-4 110-111 9.34584243190035E-5 0.0 0.0 5.415051198861303 3.2710448511651227E-4 112-113 9.34584243190035E-5 0.0 0.0 5.868207733778071 3.2710448511651227E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4025 0.0 64.418884 1 GTATCAA 7365 0.0 50.986786 1 ATCAACG 9445 0.0 40.04203 3 TCAACGC 9665 0.0 39.06895 4 CAACGCA 9710 0.0 38.786633 5 TATCAAC 9690 0.0 38.47734 2 AACGCAG 10095 0.0 37.661026 6 GTACATG 9785 0.0 32.894463 1 TACATGG 9990 0.0 31.598383 2 ACGCAGA 12110 0.0 30.952375 7 CGCAGAG 12050 0.0 30.810244 8 TGGTATC 2090 0.0 30.492424 2 ACATGGG 10035 0.0 30.09088 3 GCAGAGT 13205 0.0 26.898209 9 GTGGTAT 2475 0.0 26.491547 1 GAGTACT 7795 0.0 25.951378 12-13 CATGGGG 4305 0.0 24.349152 4 CGCGGAA 375 0.0 23.798384 82-83 CATGGGA 6220 0.0 23.74683 4 AGAGTAC 12365 0.0 23.480814 10-11 >>END_MODULE