FastQCFastQC Report
Thu 26 May 2016
SRR1364583_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364583_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1358156
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31560.2323738951931884No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22490.16559217056067196No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15990.11773316172810783No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14770.10875039391645733No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA58900.042.7297631
GGTATCA36400.039.7200971
TACCTGG11450.038.9713252
GTACATG82800.038.012881
TACATGG84150.036.5532382
CAACGCA69150.035.3621835
TCAACGC69000.035.2666054
ATCAACG69400.035.1490673
AACGCAG71300.034.542386
ACATGGG86800.034.0663873
ATCGCGT350.002790016633.99909618-19
TATCAAC72700.033.9627722
ACGCAGA81400.029.9640777
CGCAGAG80750.029.4728988
CATGGGG32300.027.6298274
GTACCTG16800.027.6242641
CATGGGA54300.027.501944
GAGTACA63250.026.9036311
AGTACAT60300.026.4427682
GCAGAGT85350.026.281089
Produced by FastQC (version 0.11.5)