FastQCFastQC Report
Thu 26 May 2016
SRR1364583_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364583_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1358156
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60110.44258538783468176No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52210.38441828479202683No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35240.2594694571168555No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15620.11500887968686954No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46200.049.1741031
GTATCAA75550.044.9877051
ATCAACG98200.034.3366553
TCAACGC98550.033.852014
TATCAAC100200.033.4729272
CAACGCA99050.033.458755
GTACATG85150.032.772881
AACGCAG102000.032.3744056
TACATGG85600.031.457032
ACATGGG86800.029.9926413
ACGCAGA117600.027.776287
GAGTACT67300.027.5382312-13
CGCAGAG118500.027.4146888
TACCTGG11600.027.2189222
GTGTACG1556.718992E-626.9289741
CATGGGG31050.026.6689664
AGTACTT70250.026.12774312-13
TATAACG1608.6594955E-626.06342
GCAGAGT123650.025.2623799
GTACTTT75150.024.66164814-15
Produced by FastQC (version 0.11.5)